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Brilliant Calcium Kits
High-throughput, no wash calcium (Ca²+) assay. An optimal solution for measuring intracellular Ca²+ dynamics, including effectors such as GPCRs and protein transporters.
ION Biosciences’ Brilliant Calcium (Ca²+) Assay is a total assay solution for multi-well plate-based, high-throughput measurements of dynamic changes in intracellular Ca²+. The ION Biosciences’ Brilliant Calcium Assay is useful for a wide range of effectors of intracellular Ca²+ including many G protein-coupled receptors (GPCRs), divalent cation channels, and other proteins capable of transporting Ca²+ or affecting intracellular Ca²+ dynamics. In multi-well, plate-based formats, the Brilliant Calcium Assay can be used to discover and characterize the effects of tens to many tens-of-thousands of compounds and environmental factors which can act to affect intracellular Ca²+ levels.
High-throughput, no wash calcium (Ca²+) assay. An optimal solution for measuring intracellular Ca²+ dynamics, including effectors such as GPCRs and protein transporters.
ION Biosciences’ Brilliant Calcium (Ca²+) Assay is a total assay solution for multi-well plate-based, high-throughput measurements of dynamic changes in intracellular Ca²+. The ION Biosciences’ Brilliant Calcium Assay is useful for a wide range of effectors of intracellular Ca²+ including many G protein-coupled receptors (GPCRs), divalent cation channels, and other proteins capable of transporting Ca²+ or affecting intracellular Ca²+ dynamics. In multi-well, plate-based formats, the Brilliant Calcium Assay can be used to discover and characterize the effects of tens to many tens-of-thousands of compounds and environmental factors which can act to affect intracellular Ca²+ levels.
Brilliant Calcium Flex Kit
Our Flex kit provides all necessary reagents in a format to maximize flexibility for your assay design and customization challenges.
Contents of Brilliant Calcium Flex Kit
Our Flex kit provides all necessary reagents in a format to maximize flexibility for your assay design and customization challenges.
Contents of Brilliant Calcium Flex Kit
|
Label |
Name |
Volume |
Containers |
Storage |
|
Reagent A |
Brilliant Calcium
Reagent |
Dry |
10 |
-20° C |
|
Reagent B |
DMSO |
225 μL |
1 |
4° C |
|
Reagent C |
DySolv |
4 mL |
1 |
4° C |
|
Reagent D |
10X Assay Buffer |
20 mL |
1 |
4° C |
|
Reagent E |
TRS |
4 mL |
1 |
4° C |
|
Reagent F |
Probenecid Solution |
4 mL |
1 |
4° C |
Brilliant Calcium Express Kit
Our Express kit is custom tailored to customer’s needs, providing only the reagents you need in the amounts you need them. Less waste, higher reproducibility, and more convenience.
*Research or further manufacturing use only, not for food or drug use.
Item#:
ASBRILLIANTCAL
ION Biosciences IPG-2 AM
Yellow-green fluorescent, potassium (K+) indicator, membrane permeable.
Lower K+ affinity than IPG-4 and higher affinity than IPG-1
ION Potassium Green – 2 (IPG-2) is a yellow-green fluorescent, intracellular potassium (K+) indicator with Ex/Em: 525 nm/545 nm and a high-sensitivity to detect small changes in K+ concentration. IPG-2 has a higher affinity (Kd = 18 mM) than IPG-1 (Kd = 50 mM) and lower affinity than IPG-4 (Kd = 7 mM).
ION Biosciences Fluo-3 K+ Salt
Green fluorescent, calcium (Ca²+) indicator, membrane impermeable
Fluo-3 is a green fluorescent, intracellular calcium (Ca²+) indicator with slightly red-shifted spectral properties compared to Fluo-4. Offers a large dynamic range that can be better suited for high contrast imaging applications.Is preferred over Fluo-4 for some cell types.
Fluo-3 K+ salt is a membrane impermeable form of Fluo-3 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.
Item#:
ASFLUO3AMST
ION Biosciences ICR-1 K+ Salt
ION Calcium Red – 1 (ICR-1) is a red fluorescent (Ex/Em 580nm/660nm) calcium (Ca²) indicator for intracellular Ca²+ measurements. ICR-1’s long-wavelength emission and a large Stokes shift reduces contributions of autofluorescence, making ICR-1 AM optimal for cellular and tissue imaging applications. ICR-1 can also be multiplexed with GFP-labeled cells or other green fluorophores commonly used in experiments. Unlike some other red fluorescent Ca²+ indicators, ICR-1 does not accumulate in the mitochondria. Data supporting fluorescence lifetime imaging and multiphoton imaging capabilities have also been demonstrated.
ION Calcium Red – 1 (ICR-1) is a red fluorescent (Ex/Em 580nm/660nm) calcium (Ca²) indicator for intracellular Ca²+ measurements. ICR-1’s long-wavelength emission and a large Stokes shift reduces contributions of autofluorescence, making ICR-1 AM optimal for cellular and tissue imaging applications. ICR-1 can also be multiplexed with GFP-labeled cells or other green fluorophores commonly used in experiments. Unlike some other red fluorescent Ca²+ indicators, ICR-1 does not accumulate in the mitochondria. Data supporting fluorescence lifetime imaging and multiphoton imaging capabilities have also been demonstrated.
ICR-1 K+ salt is a membrane impermeable form of ICR-1 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.
Item#:
ASICR1KST
ION Biosciences IPG-2 TMA+ Salt
Yellow-green fluorescent, potassium (K+) indicator, membrane
Yellow-green fluorescent, potassium (K+) indicator, membrane
impermeable. Lower K+ affinity than IPG-4 and higher affinity than IPG-1
ION Potassium Green – 2 (IPG-2) is a yellow-green fluorescent, intracellular potassium (K+) indicator with Ex/Em: 525 nm/545 nm and a high-sensitivity to detect small changes in K+ concentration. IPG-2 has a higher affinity (Kd = 18 mM) than IPG-1 (Kd = 50 mM) and lower affinity than IPG-4 (Kd = 7 mM).
IPG-2 TMA+ salt is a membrane impermeable form of IPG-2 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods
Item#:
ASIPG2TMAST
SBFI AM
Ratiometric sodium (Na+) indicator, membrane permeable
SBFI is a UV-excitable, ratiometric green indicator for intracellular sodium (Na+) measurements. Ex/Em: 340/505 nm can be used to measure Na+-bound SBFI, and Ex/Em: 380/505 nm can be used to detect Na+-free SBFI. It is ~18X more selective for Na+ over K+. Ratiometry is optimal for imaging applications where quantification of intracellular Na+ concentrations is desired, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.
Item#:
ASSBFIAM
ION Ethidium Homodimer I
Red fluorescent, dead cell stain.
Ethidium homodimer I (EthD-I) is a membrane-impermeable, high-affinity, nucleic acid stain that selectively labels dead cells with compromised plasma membranes.
ION Biosciences IPG-4 TMA+ Salt
Yellow-green fluorescent, potassium (K+) indicator, membrane
impermeable. Higher K+ affinity than IPG-1 and IPG-2.
ION Potassium Green – 4 (IPG-4) is a yellow-green fluorescent, intracellular potassium (K+) indicator with Ex/Em: 525 nm/545 nm and a high-sensitivity to detect small changes in K+ concentration. IPG-4 has the highest affinity (Kd = 7 mM) among IPG analogues – IPG-2 (Kd = 18 mM) and IPG-1 (Kd = 50 mM).
IPG-4 TMA+ salt is a membrane impermeable form of IPG-4 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods. IPG-4 has been successfully used as a sensor for extracellular K+.
Item#:
ASIPG4TMAST
Thallos AM
Best in class, green fluorescent, thallium (Tl+) indicator, membrane permeable. Works with potassium (K+), sodium (Na+), and monovalent cation channels, and transporters.
Thallos is a green fluorescent, intracellular thallium (Tl+) indicator, and has been the gold standard for fluorescence-based potassium (K+) channel HTS for nearly 2 decades. Thallos also delivers outstanding results for a wide variety of monovalent cation (sodium – Na+) channels, transporters, and GPCRs.
Item#:
ASTHALLOSAM
ION Biosciences Fluo-2 K+ Salt
Green fluorescent, calcium (Ca²+) indicator, membrane impermeable. Higher Ca²+ affinity than Fluo-4.
Fluo-2 is a green fluorescent, calcium (Ca²+) indicator. Fluo-2 has higher Ca²+ affinity (Kd = 290 nM), superior cell loading and comparable spectral properties compared to Fluo-4. Offers superior sensitivity versus other Fluo analogues at lower Ca²+ concentrations.
Fluo-2 K+ salt is a membrane impermeable form of Fluo-2 that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.
Item#:
ASFLUO2AMST
ION Biosciences Fura-2 LR K+ Salt
Leakage-resistant, ratiometric calcium (Ca²+) indicator, membrane impermeable.
UV-excitable, ratiometric green indicator for intracellular calcium (Ca²+) measurements with longer cellular retention compared to Fura-2 AM and similar spectral properties. Ex/Em: 340/505 nm can be used to measure Ca²+-bound Fura-2, and Ex/Em: 380/505 nm can be used to detect Ca²+-free Fura-2. Ratiometry is optimal for imaging applications where quantification of intracellular Ca²+ concentrations is desired, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology.
Fura-2 LR K+ salt is a membrane impermeable form of Fura-2 LR that can be used in lipid membrane-free systems, in liposomes, or can be introduced into cells by electroporation, microinjection or other methods.
Item#:
ASFURA2LRKST
ION Biosciences BCECF AM
BCECF AM is the most popular green fluorescent, intracellular pH indicator. BCECF has a pKa of ~7, and exhibits pH-dependent, dual-excitation properties (Ex/Em 430nm/535nm and 490nm/535nm) for ratiometric analysis. Ratiometry is optimal for imaging applications, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology. For HTS applications, BCECF can also be used in non-ratiometric mode using standard fluorescein excitation and emission settings.
BCECF AM is the most popular green fluorescent, intracellular pH indicator. BCECF has a pKa of ~7, and exhibits pH-dependent, dual-excitation properties (Ex/Em 430nm/535nm and 490nm/535nm) for ratiometric analysis. Ratiometry is optimal for imaging applications, and reduces effects of photobleaching, heterogenous dye loading, and variable cell morphology. For HTS applications, BCECF can also be used in non-ratiometric mode using standard fluorescein excitation and emission settings.
Item#:
ASBCECFAMPN
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