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The RotoCycler Discs are specially made to be used with the Qiagen Rotor-Gene Q Real-Time Rotary Analyzer. Two models are available: a 72-well format with 100 µL tubes and a 100-well format for reactions up to 25 µL. The discs a"one-piece "plate" equivalent, having vertically oriented wells compatible with automated reaction setup using a robotic liquid handling system.
Please know that the manufacturer has suspended manufacturing this product indefinitely.
We suggest purchasing PR1MA PCR Plastics.
Contact your rep or email tech@midsci.com for more information.
Reusable and versatile, Axygen AxyMats minimize evaporation in a wide range of temperatures in PCR and storage applications.
Sonication Sealing Films
All films on this page are designed for PCR. If you are looking for something specific please consult our selection guides by attribute or application on this page. All films are certified DNase- & RNase-Free.
Specific film descriptions:
X-Pierce (XP-100 non-sterile and XPS-25 sterile) film is designed for short-term processing and storage of samples that require multiple access to wells by pipet tips or robotic probes. A pre-cut "X" over each well creates bendable aps that provide sample access without coring or adhesive fouling of tips or probes. Ideal for COVID-19 sample collection in deep-well plates using swabs.
- Pierceable
· Printed circles aid in the alignment of the film to the plate.
· Printed alphanumerics identify each well of a 96-well plates.
· Reseals after piercing for continued sample protection.
ThermalSeal® 2™ and ThermalSeal® Advanced Sealing Film
- Certified DNase- & RNase- free
- Ideal for use with flat-top, semi-skirted and full-skirted PCR plates
- Compatible with hard-shell dual component (e.g. polycarbonate/polypropylene) PCR plates
- Large cut allows for easier positioning of the film to the plate via edge of plate alignment vs. centering
- Available in a smaller cut for raised-rim and non-skirted PCR plates: ThermalSeal® Advanced
- PCR
- -40°C to +120°C
- Selection of the proper film size will minimize evaporation
- The film should not overhang or ride up the wall of a raised-rim plate
- More complete coverage of skirted flat-top plates will reduce evaporation in the outer wells
- End-tab removal before thermal cycling is highly recommended
ThermalSeal® RT2™ Optically Transparent, Highly Conformable
Adhesive, Polyester Sealing Film
Designed for use with standard 96-well plates where rows must be selectively protected or accessed. Available in a smaller cut for raised-rim and non-skirted PCR plates: ThermalSeal® RT2RR
- Certified DNase- & RNase- free
- Ultra-high optical clarity
- Minimizes evaporation and cross-contamination
- Ideal for use with flat-top and hard-shell dual component (e.g. polycarbonate / polypropylene) PCR plates
- Not recommended for removal after thermal cycling
Application
- qPCR
- -40°C to +120°C
Helpful Tips
- Selection of the proper film size will minimize evaporation
- The film should not overhang or ride up the wall of a raised-rim plate
- More complete coverage of skirted flat-top plates will reduce evaporation in the outer wells
- End-tab removal before thermal cycling is highly recommended
ThermalSeal® RTS™ Optically Clear, Pressure Activated, Non-tacky
Silicone Adhesive
The silicone adhesive is non-tacky until pressure is applied to the sealing surface which then creates a secure bond around the wells and perimeter of the plate. Also ideal for dry samples (e.g. soil, seeds, insects, etc.) because the non-activated adhesive above the wells remains non-tacky. A roller or sealing paddle is highly recommended for use with this product to ensure consistent and adequate pressure is applied during the sealing process.
- Provides an extremely strong seal to minimize evaporation
- Certified DNase- & RNase- free
- Chemically resistant to DMSO, acetonitrile, and methanol
- Ideal for use with flat-top, raised-rim and non-skirted PCR plates
- High optical clarity and low auto-fluorescence
- Available in roll format for automation: ThermalSeal® RTS™ Roll-Seal
- A complimentary sealing paddle is included in each box
Applications
- qPCR
- Long-term storage
- Protein crystallography
- HPLC
- -80°C to +105°C
Helpful Tips
- Sufficient pressure must be applied to activate the silicone adhesive
- Multiple swipes of the sealing paddle in both directions is highly recommended
- A 10 minute or greater wait period before thermal cycling increases the film-to-plate bond
ThermalSeal® An Easier to Remove Sealing Film
ThermalSeal® features a moderate strength adhesive for ease of removal.
- Certified DNase- & RNase- free
- Only recommended for structurally rigid polypropylene PCR plates that don’t warp
- A less rigid polypropylene film that minimizes aerosols upon removal
- Features good chemical resistance
- PCR
- General storage
- -40°C to +120°C
Product Info
- Minimizes evaporation, prevents well-to-well contamination and spillage
- Eliminates the “edge effect” which can occur with plate lids (e.g. greater evaporation in the outside plate wells)
- Provides a secure seal and is easy to remove
- Rigid polyester film features good clarity and easy handling
- Can be used as a temporary economical seal while awaiting further processing
- Features two end-tabs with perforations
Permanently label mutliple microplates with clear Well Orienter Stickers. Each well is clearly marked with its corresponding row number and column letter for easy cross-referencing. The paper backing is scored in the middle allowing for easy aligning on the underside of the plate.
U of M: 50/pack
Layout: 96-well
Description: Made of clear polyester with acrylic adhesive
A convenient holder for a microplate, tilted at a 30° angle to comfortably view samples and pipette without strain. Each stand has a neoprene pad and rubber mounting to prevent slippage of the plate and stand.
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