PCR/qPCR

MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more.  If you are looking for any of these things, we can match almost all needs to all cyclers.  If you don’t find it easily contact us at tech@midsci.com and we will find it for you. 

96 or 384 we have it.  Non-Skirted, semi-skirted or full skirted, we have it.  0.1, 0.2, or 0.5 mL we have it.  A1, A12, H12, A24, P24 cuts, we have them.  Sybr© Green or probe alternative, we have it.  

Pro Tip: Colored plastics do not have any impact on DNA amplification.  But when setting up qPCR it is often recommended to use white plastics.  This will limit or prevent fluorescence refraction.  Light refraction can minimize sensitivity and consistency. 

Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips.  This will help optimize and reduce variance in the reactions.  Simple test, see the residual liquid left in tips?  This means some of the taq, dNTPs, sample, etc is left in the tip.  PR1MA tips help reduce that significantly and improve your consistency. 

Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate?  Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler.  Call us we have solutions.

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AbsorbMax Black Vinyl Sealing Film

AbsorbMax is a black vinyl sealing film for black microplaes that can be applied to either the top or bottom of the plate.

  • Minimizes background fluorescence and cross talk between plate wells
  • Ideal as a back seal for black plates with clear well bottoms for top reading measurements
  • Absorbs stray light, reducing the background caused by light scatter
  • Protects light sensitive samples from degradation
  • Features a single split liner for easy application

Applications

  • Fluorescence assays
  • Light sensitive sample protection

Temp. Range

  • -40°C to +80°C

Proudly made in the USA! Click here for more made in America products! 

BrightMax Sealing Film

White, light-reflecting, vinyl sealing film designed for use with white microplates.

  • The light reflective properties on clear-bottom plates maximize the sensitivity of bioluminescence and chemiluminescence assays in bottom-reading luminometers
  • Maximizes intensity and signal-to-noise ratios in luminescence assays
  • May be used as a backing matrix for microscopy of punch-out samples for filter plates (e.g. ELISPOT assays)
  • Features a single split liner for easy application

Applications

  • Luminescence
  • Fluorescence
  • Microscopy
Temp. Range
  • -40°C to +80°C

Proudly made in the USA! Click here for more made in America products! 

Plate-Sealing Roller

Application: Plate-sealing

Material:
Rubber, metal

The plate-sealing roller features a smooth spinning, thermoplastic elastomer sealing surface with a durable plated metal handle.

  • Reusable, autoclavable
  • Provides a large 4 inch (10.16 cm) wide sealing surface
  • A 10 minute or greater dwell me at room temperature before processing increases the film-to-plate bond

Proudly made in the USA! Click here for more made in America products! 


Accuris Fast Extraction PCR Kit

 

The Accuris Fast Extraction PCR Kit features an exclusive single-tube solution that extracts genomic DNA in just 8 minutes. This is swiftly followed by PCR amplification utilizing our High Fidelity Hot Start Red Dye Master Mix, designed for superior endpoint PCR performance. High Fidelity Hot Start Red Dye Master Mix is a 2X formula that incorporates a non-reactive red dye, enabling direct gel loading.

 

This kit offers versatility to suit different workflows: the PCR-prepared genomic DNA can be directly used in endpoint PCR or can be applied in real-time PCR using either SYBR Green or TaqMan Probe chemistries.

  • Offers exceptional versatility and can be used to extract and amplify DNA from a wide range of sample types
  • Compatible with animal tissue, plant, saliva, & bacterial samples
  • Extraction & Amplification in less than 60 minutes
  • Includes High Fidelity Hot Start Red Dye Master Mix to prevent the risk of nonspecific amplification & to visualize bands easily
  • Process extracted sample via PCR with included mastermix
  • Alternatively, amplify via qPCR (qPCR mastermix not included) 

Each 100 reaction kit includes:

1 tube of High Fidelity Hot Start Red Dye Master Mix (1.25 mL)

1 tube of Fast Extraction Lysis Solution (1.25 mL)

 

Applications: Extraction & Amplification of Tissue, Plant, Saliva, & Bacteria
 

Proudly made in the USA! Click here for more made in America products!
 


Accuris qMAX SYBR Green Bulk Packaging


Supplied as a ready-to-use 2X master mix, qMax Green has been engineered for high sensitivity, fast cycling and excellent reproducibility.
  • Superior sensitivity and fast cycling with exceptional results.
  • Ideal for low copy number templates.
  • Early Ct values and detection across a broad dynamic range.
  • Ready to use 2x mastermix.
  • Includes Accuris Hot Start Taq Polymerase for greater specificity and accuracy.
Supplied as a ready-to-use 2X master mix, qMax Green has been engineered for high sensitivity, fact cycling and excellent reproducibility. Accuris Hot Start Polymerase provides accurate PCR of a variety of templates including low copy number and difficult sequences, while the proprietary qMax Green intercalating dye exhibits higher fluorescence and lower PCR inhibition than other popular green dyes.

These two components are supported by a specially formulated buffer with an exacting combination of salts, PCR enhancers, stabilizers and pH that results in earlier Ct values and a high specificity across a broad dynamic range.

Bulk Packaging:
To accommodate the requirements of high throughput users, Accuris is now  offering their full line of qPCR and RT-qPCR reagents in bulk packaging at highly discounted prices. Bottle sizes of 25 mL (2500 reactions) and 50 mL (5000 reactions) are available for qMAX SYBR Green and qMAX Probe mixes. One Step RT-qPCR kits are also available in bulk format for 2500 and 5000 reactions and are ideal for labs performing pathogen detection.

Please Note:
Low Rox and High Rox formulations are available for compatibility with all brands of qPCR cyclers.  Please specify when ordering.  
All PCR reagents ship in protective coolers with frozen gel packs.

Proudly made in the USA! Click here for more made in America products!
 

Limited Time Promo!


2025 LabValues Promotion!
For a limited time enjoy discounted pricing on the
ENDURO™ Power Supply Series (E0303 and E0203)!

 

See flyer for more details.

 

Special price applies only to the specified item; no substitutions.

Cannot be combined with other discounts or pricing. Not valid on prior purchases.

Promo is valid 01/06/2025 – 06/30/2025 or while supplies last.

All terms and conditions apply.


ENDURO Power Supply 

 

  • Two models (300V and 250V) available
  • For DNA, RNA, or protein electrophoresis, and blotting applications
  • Constant voltage or constant current with automatic crossover
  • 250 V model also offers a constant power operating mode
  • Timed or continuous operation
  • Short circuit and over current/ over voltage protection
  • No load and load change detection system
  • Programming includes option for automatic recovery after power failure
  • 3 year warranty

 

The new Enduro Power Supplies meet a wide range of applications including DNA, RNA, and protein electrophoresis, blotting, and many others. Two models are available with voltage ranges to 300V and current ranges to 3000mA. Operating modes include constant voltage or constant current with automatic crossover. In addition, the 250V model offers a constant power operating mode.

 

All parameters, including voltage, current, power (250V model only), and time are easily set using the membrane keypad and displayed digitally on the back lit LCD. Compact in design with a small footprint, the Enduro Power Supplies are also stackable to save space. For easy viewing and setting of parameters on the bench or on a shelf, the front of the power supply can be raised with the integral folding feet.

Specifications

Model300V250V
Output Voltage Range/Increments2-300V/1V5-250V/1V
Output Current Range/Increments4-500mA/1mA10-3000mA/10mA
Output Power Range/Increments90 watts max/n.a.1-300W/1W
Operating Constant Modescurrent or voltagecurrent, voltage, or power
Timer1 -99 min, or continuous1min. to 99h 59min or continuous
ProgrammablenoYes (20 program capacity)
Dimensions (W x D x H)19 x 25 x 8 cm19 x 25 x 8 cm
Weight2.6 lbs5.7 lbs
Input Voltage110-230 V110-230 V
Item#:
ASPOWERSUP1


2025 LabValues Promotion!
For a limited time enjoy discounted pricing on select
Labnet Enduro™ UV Transilluminators (U1001 and U1002)!

 

See flyer for more details.

 

Special price applies only to the specified item; no substitutions.

Cannot be combined with other discounts or pricing. Not valid on prior purchases.

Promo is valid 01/06/2025 – 06/30/2025 or while supplies last.

All terms and conditions apply.


Enduro UV Transilluminators


The NEW ENDURO UV Transilluminator represents a great new imaging product true to ENDURO name. This UV transilluminator has been designed for rugged use and durability with exceptional performance.
  • Compact size with 21 x 26 cm viewing area
  • Available in single or dual wavelength models
  • Unique heavy duty hinge design can be held in place at any angle for gel cutting
  • UV shield fully protects user from UV light
  • Uniform UV Black Glass provides improved imaging contrast
 
The use of UV Black Glass minimizes the striping effect seen on many UV transilluminators. This provides a more uniform background for gel imaging.
Item#:
ASENDUROUVTRANS

PurePlus® PCR Plates

  • PurePlus® plates are tested for RNase/DNase lot by lot to ensure pure product
  • Our plates are lot certified free of Human DNA contamination
  • Our plates are tested for ATP lot by lot to ensure pure consistent product
  • Broad product range. Over 18 styles of plates fit most major thermocyclers
  • These plates are autoclavable to 122° C for 15 minutes at 15PSI

Ultra Clear and Ultra Clean
We make our PurePlus® Polymerase Chain Reaction (PCR) consumables without additives so you don't need to wonder what is different about the plates, tubes, or strips you are using this week. We also test every batch for contamination by Human DNA as well as the more common tests that other suppliers offer for RNase and DNase enzymes. Many of our plates are printed with black alpha numeric reference grids to make it easier for you to keep track of your samples. We also offer optically clear caps and opaque black or white plates for Real Time PCR. And we also offer re-usable silicone sealing mats and polypropylene sealing film. Labcon PurePlus® PCR tubes, strips and plates are designed to fit many popular thermocyclers including brands like Applied Biosystems, Eppendorf, Thermo Scientific, and Bio Rad. All our PCR disposables are made from 100% virgin polypropylene and are lot tested and certified to be free of RNase, DNase, and Human DNA. Autoclavable. Our pure resin consumables are available in strips, individual tubes, plates in clear natural or colors.

Our PurePlus® sterile disposables, like all our other sterile products, receive valid sterilization processing compliant with ISO 11137. So unlike "pre-sterile" products from other suppliers you can use PurePlus® PCR disposables for diagnostic and regulated procedures.
 

MIDSCI # VWR #
3983-520-000-9 82006-678
3983-520-010-9 89218-288
3984-520-000-9 89218-294
3961-520-000-9 89218-296
3962-520-000-9 89218-298
3962-521-000-9 10755-256
3971-520-000-9 82006-650
3976-520-000-9 82006-664
3978-520-000-9 82006-704
3978-520-010-9 89218-292
3978-522-000-9 82006-714
3978-524-000-9 82006-710
3978-526-000-9 82006-712
3978-527-000-9 82006-706
3978-528-000-9 82006-708
3978-529-000-9 82006-718
3963-520-000-9 83009-676
3967-520-000-9 83009-668
3968-520-000-9 83009-672
3972-520-000-9 89049-178
3972-520-010-9 89218-290
3973-520-000-9 83007-374
3973-529-000-9 83007-372
3974-520-000-9 37001-272
3977-520-000-9 82006-636
3977-522-000-9 82006-646
3977-526-000-9 82006-644
3977-527-000-9 82006-638
3977-528-000-9 82006-640
3977-529-000-9 82006-648
3979-520-000-9 93001-100
Item#:
ASLABPUREPLS
96x0.1ml, Sub-Skirted,
This item has been discontinued and replaced by item 
  • Plate Characteristics
    • 96 x 0.1mL, Sub-skirted
    • Low Profile (0.1mL), Frosted (low signal to noise ratio
    • Extreme Uniformity (EU) Plate Design
    • Uniform wall thickness ensures consistent even heating
    • Thin wall-bottomed tube for rapid heat transfer
  • Ideal paring with EU Caps with "Sealing Ring Band" Design
    • Easy open - easy close with ergonomic caps: B57801, B79701, C79701
    • Caps are significantly easier to apply and remove -- Repetitive Strain Injury (RSI) Friendly.
    • Significant reduction in evaporation due to a wider sealing area.
  • Certified DNA, DNase, RNA, RNase and pyrogen-free

Please give us a call for a sample!

 

Order#

Description

Non-Skirt

 

B70501

Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, Natural, 25/cs

B70509

Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, White, 25/cs

B50501

Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, Frosted, 25/cs

AB70651

Pryme PCR Plate, 96-Well, 0.1mL, Non-Skirt, Low Profile, Frosted, 25/cs

Semi-Skirted

 

B70651

Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, Natural, 25/cs

B70659

Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, White, 25/cs

B50651

Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, Frosted, 25/cs

B17480

Pryme PCR Plate, 96-Well, 0.1mL, Semi-skirted, Low Profile, Frosted, 25/cs

B17489

Pryme PCR Plate, 96-Well, 0.1mL, Semi-skirted, Low Profile, White, 25/cs

Sub-Skirted

 

ABI7501

Pryme PCR Plate, 96-Well, 0.2mL, Sub-skirted, Regular Profile, Frosted, 25/cs

ABI7509

Pryme PCR Plate, 96-Well, 0.2mL, Sub-skirted, Regular Profile, White, 25/cs

AB19800-EU

Pryme PCR Plate, 96-Well, 0.1mL, Sub-skirted, Low Profile, Frosted, 25/cs

AB19809-EU

Pryme PCR Plate, 96-Well, 0.1mL, Sub-skirted, Low Profile, White, 25/cs

Caps

 

B57801

Pryme PCR Ergonomic 8-Strip, Optical Flat (V2) Caps, Natural, 120/cs

B79701

Pryme PCR Ergonomic 8-Strip, Optical Flat (V1) Caps, Natural, 120/cs

C79701

Pryme PCR Ergonomic 8-Strip, Semi-Domed (V1) Caps, Natural, 120/cs

Item#:
AB19809-EU
Your Price:
158.24
Each
This item is discontinued
Skirt, PCR, Clear, 10/pk
This item has been discontinued and replaced by item 
Please know that the manufacturer has suspended manufacturing this product indefinitely.
We suggest purchasing 
PR1MA PCR Plastics.
Contact your rep or email tech@midsci.com for more information.


 
  • Standard thermal cycling and qPCR compatible
  • Ultra-thin and consistent wall thickness for precise thermal transfer
  • Automation friendly, single notch corner, stackable
  • Made from 99.9% virgin polypropylene
  • 240ul well capacity (working capacity dependent on closure method)
  • Barcoding available
  • Certified DNAse/RNAse and pyrogen-safe
Item#:
PCR-96-FS-C
Your Price:
60.39
Each
This item is discontinued
Amplification Plate
Please know that the manufacturer has suspended manufacturing this product indefinitely. 
We suggest purchasing PR1MA PCR Plastics.
Contact your rep or email tech@midsci.com for more information.
 

  • Standard thermal cycling and qPCR compatible
  • Ultra-thin and consistent wall thickness for precise thermal transfer
  • Single notch corner
  • Made from 99.9% virgin polypropylene
  • 340ul well capacity (working capacity dependent on closure method)
  • Fits standard thermal cyclers, plus ABI PCR 2700, 2720, 9700, 9800, 7300, 7500, 7900, and ABI sequencing units
  • Certified DNAse/RNAse and pyrogen-safe
Item#:
PCR-96M2-HS-C
Your Price:
42.96
Each
This item is discontinued
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