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PCR/qPCR
MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more. If you are looking for any of these things, we can match almost all needs to all cyclers. If you don’t find it easily contact us at tech@midsci.com and we will find it for you.
96 or 384 we have it. Non-Skirted, semi-skirted or full skirted, we have it. 0.1, 0.2, or 0.5 mL we have it. A1, A12, H12, A24, P24 cuts, we have them. Sybr© Green or probe alternative, we have it.
Pro Tip: Colored plastics do not have any impact on DNA amplification. But when setting up qPCR it is often recommended to use white plastics. This will limit or prevent fluorescence refraction. Light refraction can minimize sensitivity and consistency.
Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips. This will help optimize and reduce variance in the reactions. Simple test, see the residual liquid left in tips? This means some of the taq, dNTPs, sample, etc is left in the tip. PR1MA tips help reduce that significantly and improve your consistency.
Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate? Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler. Call us we have solutions.
1 x 8 FilmStrips™
For Sealing Only One 8-well Row of a 96-well Plate.
Designed for use with either 1×8 strip-well ELISA plates or standard 96-well plates where rows must be selectively protected or accessed. Ideal for COVID-19 testing to seal a row of 8 wells after swabs are deposited into a deep-well plate.- Certified DNase- & RNase- free
- A pliable polypropylene film minimizes aerosols upon removal
- Each sheet contains 8 strips on a continuous liner for ease of use
- Apply individually or two-at-a-time
- Use as a secondary film to seal previously pierced wells or to prevent accidental pipetting
- General laboratory use
- Incubation
- ELISA
Adhesive Sealing Film, Polyolefin
These 2.8 mL, optically clear polyester films are ideally suited for Real Time PCR (qPCR). They feature an inert, ultra-clear, and strong encapsulated silicone adhesive that provides extraordinary performance in real-time PCR applications.
Encapsulated silicone greatly reduces the risk of sample contamination due to exposure to adhesive. Once the film is pressed onto the plate, the raised portion of the well penetrates the encapsulation and a tight seal is formed on just that surface (not over the sample). This silicone seal adheres aggressively and diminishes the possibility of "Edge Effect".
The plasticized backing of this film is scored on both ends to allow for two handed (precise) placement on the plate. Both film and adhesive maintain incredible clarity and are the choice for highly demanding manual and automated processes.
Certified free of RNase, DNase, Pyrogen, and nucleic-acid contamination.
Specifications
Length | 141.27 mm |
Length (w/ end tabs removed) | 117.5 mm |
Width | 77.8 mm |
Overall Thickness | 2.8 mL |
Adhesive Thickness | 2.0 mL |
Material | Polyester |
Adhesive | Encapsulated Silicone |
Max. Temperature | 120 °C |
Min. Temperature | -40 °C |
Primary Usages | Real Time PCR (qPCR), PCR |
Accuris MyVolt™ Mini Power Supply
- Compact and simple
- Choose constant 100 V or 200 V output
- Two pairs of output terminals
The Accuris MyVolt™ Mini is a compact electrophoresis power supply ideal for basic, constant voltage applications. Users can select constant 100 V or 200 V output, and the current can reach a maximum of 200 mA.
Two sets of output jacks are available to connect up to 2 gel rigs and these small power supplies can securely be stacked for use with additional gel rigs.
Specifications
Output Voltage | 100V/200V constant |
Current Output | Max. 200mA |
Max Wattage | 40W |
Output Terminals | 2 pairs |
Weight | 0.9 lbs / 0.4 kg |
Dimensions | 6.25 x 4.5 x 2.3 in. 16 x 11.5 x 5.7 cm |
Electrical | 100 - 240V, 50-60Hz |
Hoefer® Tall Standard Dual Cool Vertical Protein Electrophoresis System
Taller vertical format accommodates 24 cm long gels for increased resolution.
- Maximum glass plate size 18 x 24 cm
- Produce straight lanes and sharp, well-defined bands
- Run up to four gels (maximum 112 samples) at one time under identical conditions
- Run gels at uniform temperature from 1 to 45°C
- Accommodates denaturing and native polyacrylamide gels and 2-D electrophoresis
- A wide array of accessories allows you to tailor gel configurations to your needs
Hoefer® Tall Standard Complete Unit (SE660-15-15)
- Lower Buffer Chamber, 1/ea
- Upper Buffer Chamber, 1/ea
- Safety Lid w/High Voltage Leads, 1/ea
- Heat Exchanger, 1/ea
- Glass Plates 18 x 24 cm, 6/pk
- Clamp Assemblies, 8 cm, 4/pk
- Clamp Assemblies, 16 cm, 4/pk
- Cams, 6/pk
- Slotted Gaskets for Upper Buffer Chamber, 2/pk
- Dual Gel Casting Stand with Leveling Base, 1/ea
- Bubble Level, 1/ea
- Laminated Gaskets for Caster, 2/pk
- Buffer Dam, 1/ea
- Spacer-Mate-Alignment Template, 1/ea
- Wonder Wedge™-Plate Separation Tool, 1/ea
- Gel Seal, 1/ea
- Combs, 15-well, 1.5 mm thick, 2/pk
- Spacers, 1.5 mm thick (2 cm wide), 4/pk
- Lower Buffer Chamber, 1/ea
Hoefer® Tall Standard Basic Unit (SE660)
The same as above without the combs and spacers. Order two SE511 combs and two sets of 24 cm long spacers separately.
Specifications
Plate Size (W x H) | 7.09 x 9.45, 7.09 x 6.30, or 7.09 x 3.15 In. 18 x 24, 18 x 16 or 18 x 8 cm |
Spacers | 0.75 mm, 1.0 mm, 1.5 mm |
Max. Power | 1000 V, 500 mA, 50 W |
Max. Temp. | 113ºF | 45°C |
Indoor Use | 39.2 to 104ºF | 4 to 40°C |
Humidity | Up to 80% |
Dims (D x W x H) | 5.51 x 12.60 x 14.57 In. | 14 x 32 x 37 cm |
Weight | 19.40 lb | 8.80 kg |
Proudly made in the USA! Click here for more made in America products!
Hoefer® Standard Dual Cooled Vertical Protein Electrophoresis System
Standard vertical format for 18 cm long gels.
- Maximum glass plate size 18 x 16 cm
- Produce straight lanes and sharp, well-defined bands
- Run up to four gels (maximum 112 samples) at one time under identical conditions
- Run gels at uniform temperature from 1 to 45°C
- Accommodates denaturing and native polyacrylamide gels and 2-D electrophoresis
- A wide array of accessories allows you to tailor gel configurations to your needs
Hoefer® Standard Complete Unit (SE600-15-15)
- Lower Buffer Chamber, 1/ea
- Upper Buffer Chamber, 1/ea
- Safety Lid w/High Voltage Leads, 1/ea
- Heat Exchanger, 1/ea
- Glass Plates 18 x 16 cm, 6/pk
- Clamp Assemblies, 16 cm, 4/pk
- Cams, 6/pk
- Slotted Gaskets for Upper Buffer Chamber, 2/pk
- Dual Gel Casting Stand with Leveling Base, 1/ea
- Bubble Level, 1/ea
- Laminated Gaskets for Caster, 2/pk
- Buffer Dam, 1/ea
- Spacer-Mate Alignment Template, 1/ea
- Wonder Wedge Plate Separation Tool, 1/ea
- Gel Seal, 1/ea
- Combs, 15-well, 1.5 mm thick, 2/pk
- Spacers, 1.5 mm thick, 2 cm wide, 4/pk
Hoefer® Standard Basic Unit (SE600)
The same as above without the combs and spacers. Order two SE511 combs and two sets of 16 cm long spacers separately.
Specifications
Plate Size (W x H) | 7.09 x 6.30, or 7.09 x 3.15 In. 18 x 16 or 18 x 8 cm |
Spacers | 0.75 mm, 1.0 mm, 1.5 mm |
Max. Power | 1000 V, 500 mA, 50 W |
Max. Temp. | 113ºF | 45°C |
Indoor Use | 39.2 to 104ºF | 4 to 40°C |
Humidity | Up to 80% |
Dims (D x W x H) | 5.51 x 12.60 x 11.42 In. | 14 x 32 x 29 cm |
Weight | 15.43 lb | 7.00 kg |
Proudly made in the USA! Click here for more made in America products!
AbsorbMax™ Black Vinyl Sealing Film
AbsorbMax™ is a black vinyl sealing film for black microplaes that can be applied to either the top or bottom of the plate.- Minimizes background fluorescence and cross talk between plate wells
- Ideal as a back seal for black plates with clear well bottoms for top reading measurements
- Absorbs stray light, reducing the background caused by light scatter
- Protects light sensitive samples from degradation
- Features a single split liner for easy application
Applications
- Fluorescence assays
- Light sensitive sample protection
Temp. Range
- -40°C to +80°C
Proudly made in the USA! Click here for more made in America products!
BrightMax™ Sealing Film
White, light-reflecting, vinyl sealing film designed for use with white microplates.
- The light reflective properties on clear-bottom plates maximize the sensitivity of bioluminescence and chemiluminescence assays in bottom-reading luminometers
- Maximizes intensity and signal-to-noise ratios in luminescence assays
- May be used as a backing matrix for microscopy of punch-out samples for filter plates (e.g. ELISPOT assays)
- Features a single split liner for easy application
Applications
- Luminescence
- Fluorescence
- Microscopy
- -40°C to +80°C
Proudly made in the USA! Click here for more made in America products!
Plate-Sealing Roller
Application: Plate-sealingMaterial: Rubber, metal
The plate-sealing roller features a smooth spinning, thermoplastic elastomer sealing surface with a durable plated metal handle.
- Reusable, autoclavable
- Provides a large 4 inch (10.16 cm) wide sealing surface
- A 10 minute or greater dwell me at room temperature before processing increases the film-to-plate bond
Proudly made in the USA! Click here for more made in America products!
For a limited time, purchase any Hoefer® SE260 Mini Gel Unit,
(SE260-10A-10, SE260-10A-15, SE260-10A-75, or SE260B)
& get 5 Hoefer® Mighty Small Precast Gels FREE!
When ordering free product, use item number plus “-VF”.
Promo valid from 6/10/25 - 7/31/25.
Offer cannot be combined with other promotions, discounts, or institutional pricing. Not valid on prior purchases.
Special price applies only to the specified item; no substitutions.
Allterms and conditionsapply.
Hoefer® Mighty Small II Deluxe Mini Vertical Electrophoresis System
Combines the advantages of small format and efficient cooling for rapid screening of proteins and nucleic acids.
- Maximum glass plate size 10 x 10.5 cm
- Flexible design, additionally able to accommodate 10 x 8 cm format with optional accessories
- Supports a wide variety of precast gels
- Efficient active cooling ensures sharp bands
- Run up to four gels at one time under identical conditions
- Quick and easy to assemble
- Choose from a wide variety of accessories
Hoefer® Mighty Small II Deluxe Complete Units (SE260-10A)
- Lower Buffer Chamber, 1/ea
- Upper Buffer Chamber/Cooling Core, 1/ea
- Safety Lid with High Voltage Leads, 1/ea
- Casting Cradle w/Sealing Gasket Set, 1/ea
- Casting Clamp Assemblies, 2/ea
- Cams, 4/ea
- Glass Plates, Rectangular, 10 x 10.5 cm, 10/ea
- Alumina Plates, Notched, 10 x 10.5 cm, 5/ea
- Spring Clamps, 4/ea
- Combs, 10 well, 2/ea
- T-Spacers, 4/ea
- Well Locating Decals, 2/ea
- Gel Seal, 1/ea
Hoefer® Mighty Small II Deluxe Basic Unit (SE260B)
The same as above without the combs, spacers, plates, and caster. For use with precast gels or order two SE211A combs, two sets of 10.5 cm long spacers, 10 x 10.5 cm rectangular glass and notched alumina plates, and a gel caster.
- Alumina Plates, Notched, 10 x 10.5 cm, 5/pk
- Spring Clamps, 4/pk
- Combs, 10 well, 2/pk
- T-Spacers, 4/pk
- Well Locating Decals, 2/pk
- Gel Seal/pk
Hoefer® Mini Electrophoresis and Western Blot Package
Save time and money with a combination package.
- SE260-10A-75 Mighty Small II Mini Deluxe Vertical Unit
- TE22 Mighty Small Mini Transfer Tank
- PS300B 300 V, 500 mA, 90 W Power Supply
Specifications
Plate Size (W x H) | 3.34 x 4.14 or 3.34 x 3.15 In. | 10 x 10.5 cm or 10 x 8 cm |
Spacers | 0.75 mm, 1.0 mm, 1.5 mm |
Max. Power | 500 V, 500 mA, 12 W |
Max. Temp. | 113ºF | 45°C |
Indoor Use | 39.2 to 104ºC | 4 to 40°C |
Humidity | Up to 80% |
Dims (D x W x H) | 6.30 x 6.50 x 7.08 In. | 16 x 16.5 x 18 cm |
Weight | 3.30 lb | 1.50 kg |
Proudly made in the USA! Click here for more made in America products!
Hoefer® Wide-Mini Vertical Protein Electrophoresis System
Extra wide format is ideal for quick, high-throughput runs. The SE640 is an extra wide vertical protein electrophoresis unit that accommodates up to 112 samples at one time.
- Maximum glass plate size 18 x 8 cm
- Permits rapid screening of up to 112 samples at one time
- Design permits gels to be surrounded uniformly by buffer so heat dissipation is efficient.
- Bands are sharp and lanes are straight
- Accommodates denaturing or native polyacrylamide gels
- Run up to four second dimension gels using IEF strips or tube gels
Hoefer® Wide-Mini Complete Unit (SE640-15-15)
- Lower Buffer Chamber, 1/ea
- Upper Buffer Chamber, 1/ea
- Safety Lid with High Voltage Leads, 1/ea
- Glass Plates 18 x 8 cm, 6/pk
- 8 cm Clamp Assemblies, 4/pk
- Cams, 6/pk
- Dual Casting Stand with Leveling Base, 1/ea
- Laminated Gaskets for Casting Stand, 2/pk
- Slotted Gaskets for Upper Buffer Chamber, 2/pk
- Buffer Dam, 1/ea
- Spacer-Mate Alignment Template, 1/ea
- Wonder Wedge Plate Separation Tool, 1/ea
- Gel Seal, 1/ea
- Bubble Level, 1/ea
- Combs, 15-well, 1.5 mm thick, 2/pk
- 1.5 mm Thick Spacers, 2 cm wide, 4/pk
Hoefer® Wide-Mini Basic Unit (SE640)
The same as above without the combs and spacers. Order two SE511 combs and two sets of 8 cm long spacers separately.
Specifications
Plate Size (W x H) | 7.09 x 3.15 In. | 18 x 8 cm |
Spacers | 0.75 mm, 1.0 mm, 1.5 mm |
Max. Power | 1000 V, 500 mA, and 50 W |
Max. Temp. | 113ºF | 45°C |
Indoor Use | 39.2 to 104ºF | 4 to 40°C |
Humidity | Up to 80% |
Dims (D x W x H) | 5.51 x 12.60 x 11.42 In. | 14 x 32 x 29 cm |
Proudly made in the USA! Click here for more made in America products!
Hoefer® Mini Horizontal Agarose Electrophoresis System
Designed for very fast separations of DNA restriction fragments in agarose gels. To achieve passive cooling, you simply fill the clear molded base with 50% ethylene glycol coolant solution, seal it, and place it in a freezer or ice bath until ready to use.
- Fast separations - run a 7 x 10 cm gel in as little as 5 minutes
- UV-transparent running tray allows the user to image the gel without risk of damage due to handling
- Variety of comb sizes enables customization of sample throughput to fit your laboratory's requirements
- Adjustable comb height offers complete control over well depth
Complete Unit (HE33-8-15)
- Buffer Chamber Assembly, 1/ea
- Safety Lid with High Voltage Leads, 1/ea
- Running Tray, 1/ea
- Casting Tray, 1/ea
- Foam Gaskets, 4/pk
- Bubble Level, 1/ea
- 1.5 mm thick, 8-well comb, 1/ea
- Comb Back, 1/ea
Basic Unit (HE33B)
The same as above, without the comb and comb back. Order separately.
Specifications
Gel Dims (W x L) | 2.76 x 3.94 In. | 7 x 10 cm |
Max. Buffer Vol. | 250 mL |
Max. Power | 500 V for (5 min or less), 500 mA, 15 W |
Max. Capacity | 32 Samples |
Max. Temp. | 122ºF | 50°C |
Indoor Use | 39.2 to 104ºF | 4 to 40°C |
Humidity | Up to 80% |
Dims (D x W x H) | 9.45 x 5.12 x 2.76 In. | 24 x 13 x 7 cm |
Weight | 1.76 lb | 0.80 kg |
Proudly made in the USA! Click here for more made in America products!
Hoefer® Submarine Gel Electrophoresis Systems
- Color-coded and height-adjustable combs easily identify comb thickness at a glance and control well depth
- Colored loading strips - for easy well detection when loading
- UV-transparent running tray allows user to image gel without risk of damage due to handling
- Buffer recirculation ports may be connected to a peristaltic pump for buffer recirculation during electrophoresis to maintain buffer pH and prevent ionic gradient formation (*Not included for SUB6 and SUB10)
- Includes
- Buffer Chamber Assembly, 1/ea
- Safety Lid, 1/ea
- High Voltage Leads, 1/ea
- Colored Loading Strips, 1/ea
- Buffer Recirculation Ports, 2/pk (*Not included for SUB6 and SUB10)
- Running Tray, 1/ea
- Combs, 1/ea
Mini (SUB6), Mini+ (SUB10) and Midi (SUB13) Submarine Gel Electrophoresis Systems
The SUB6 and SUB10 are ideal for routine nucleic acid preparatory and analytical electrophoresis techniques. SUB13 Midi is ideal for analytical and preparative studies of nucleic acids. Maximum of 32-sample (SUB6), 80-sample (SUB10), or 112-sample (SUB13) throughput capacity and features a removable gel-casting tray with end gaskets that allow gels to be cast directly within the tank.
- Compact tank reduces the buffer volume required to cover the gel, providing greater control over the voltage gradient and run-time
- Side handles for safe and easy transportation around the laboratory
- Includes
- Running Tray with Silicone Gasket*
- 1 mm thick, 8-well combs, 2/pk (SUB6 Only)
- 1 mm thick, 16-well combs, 2/pk (SUB10 and SUB13 Only)
*The included running tray is lined with silicone gaskets, which form a leak-proof seal against the inner walls of the running chamber when the running tray is turned 90° to the direction of electrophoresis.
Standard (SUB15) Submarine Gel Electrophoresis System
Provides the most commonly used tray size and comb options. Highly versatile option for screening nucleic acid samples ranging from DNA mini-preps to PCR products. 120-sample maximum throughput.
- The market standard - a 15 x 15 cm gel tray, with comb options ranging from 1 to 30 samples
- Multi-channel pipette compatible combs with a maximum 30 sample throughput reduce gel loading time, while preparatory combs enable nucleic acids to be scaled up for cloning
- Side handles for safe and easy transportation around the laboratory
- Includes
- Running Tray with Casting Gates*
- 1 mm thick, 16-well combs, 2/pk
*The included casting gates conveniently and efficiently seal the running tray without the hassles of using tape.
High Throughput (SUBHT) Submarine Gel Electrophoresis System
Ideal for high throughput screening applications like single strand polymorphism (SSP) analysis following PCR. This horizontal gel unit can resolve DNA strands from 50 – 1000 base pairs in 30 minutes and accommodate up to 120 samples (96-well plate + 24 control and/or marker samples).
- Fast, error-free loading - Load an entire 96-well plate using a multichannel pipette
- Simplified analysis - Fluorescent labels for each sample printed on a UV transparent gel tray allows for instant sample identification (matched to a 96-well plate) during post-run visualization
- Eliminate variability caused by running buffer pH drift and ionic gradient formation with optional buffer recirculation capability
- Compact tank design needs less buffer to cover gel which not only reduces buffer consumption but provides greater control of voltage gradients and run times
- Fully compatible with a wide range of combs and accessories to meet all your high throughput and standard gel analysis needs
- Includes
- Fluorescent Labeled Running Tray with Casting Gates
- 1 mm thick, 30 well combs, 4/pk
Maxi-Standard (SUB20) and Maxi-Cooled (SUB20C) Submarine Gel Electrophoresis Systems
20 x 20 cm gel-running tray with four comb positions, which allows the user to perform high-resolution analytical and preparative studies of nucleic acids. Handles up to 168 samples.
- Multichannel pipette compatible combs with a maximum 40 sample throughput reduces gel loading time
- Side handles for safe and easy transportation around the laboratory
- Includes
- Buffer Chamber Assembly with Cooling Ports (SUB20C Only)
- Running Tray with Casting Gates
- 1 mm thick, 16 well combs, 2/pk
*The included casting gates conveniently and efficiently seal the running tray without the hassles of using tape.
Maxi-Plus Standard (SUB25) and Maxi-Plus Cooled (SUB25C) Submarine Gel Electrophoresis Systems
Ideal for high-throughput screening of nucleic acids, particularly PCR products, and in genotyping studies. It has a 25 x 30 cm gel-running tray with 12 comb positions accommodating a maximum throughput of 624 samples.
- Multichannel pipette compatible combs—with a maximum 52 sample throughput, reduce gel loading time
- Side handles—for safe and easy transportation around the laboratory
- Includes
- Buffer Chamber Assembly with Cooling Ports (SUB25C Only)
- Running Tray w/Casting Gates
- 1 mm thick, 26 well combs, 6/pk