PCR/qPCR

MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more.  If you are looking for any of these things, we can match almost all needs to all cyclers.  If you don’t find it easily contact us at tech@midsci.com and we will find it for you. 

96 or 384 we have it.  Non-Skirted, semi-skirted or full skirted, we have it.  0.1, 0.2, or 0.5 mL we have it.  A1, A12, H12, A24, P24 cuts, we have them.  Sybr© Green or probe alternative, we have it.  

Pro Tip: Colored plastics do not have any impact on DNA amplification.  But when setting up qPCR it is often recommended to use white plastics.  This will limit or prevent fluorescence refraction.  Light refraction can minimize sensitivity and consistency. 

Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips.  This will help optimize and reduce variance in the reactions.  Simple test, see the residual liquid left in tips?  This means some of the taq, dNTPs, sample, etc is left in the tip.  PR1MA tips help reduce that significantly and improve your consistency. 

Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate?  Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler.  Call us we have solutions.

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ILP Aerobic Seal Sealing Film


Non-woven Rayon, acrylic adhesive, breathable, 4.9 Mil – Sterile


Breathable sealing film made from non-woven rayon with an acrylic adhesive. Designed for aerobic applications, this 4.9 mil film allows gas exchange while maintaining a sterile barrier. Ideal for cell culture and other assays requiring ventilation.

 

Specifications
 
MaterialNon-woven Rayon Acrylic
Adhesive MaterialAcrylic; 1.1 Mil
Liner MaterialPaper
ColorWhite
AlphanumericNone
CertifiedDNase-/RNase-/Pyrogen-Free
Overall Height4.9 Mil
Adhesive ThicknessAcrylic; 1.1 Mil
Top Width3.22 In. | 82.00 mm
Top Length6.06 In. | 154.00 mm
AutoclavablePre-Sterilized
Max Temp.80ºC (176ºF)
Min Temp.-20ºC (-4ºF)
 

Item#:
ASADHESEALFLALM14


ILP Polyolefin Sealing Film 

  • Optical Seal Adhesive Sealing Film
  • Polyolefin 2.8 mil Ultra-clear for qPCR
  • 50 Films/Pack, 10 Packs/Case 

Incredible optical clarity, non-absorbing, and non-fluorescing properties of these films make them ideally suited for Real Time PCR (qPCR) and optical assay work. Featuring an inert, ultra-clear, and strong encapsulated silicone adhesive that provides extraordinary performance in demanding applications.


Encapsulated silicone greatly reduces the risk of sample contamination due to exposure to adhesive. Once the film is pressed onto the plate, the raised portion of the well penetrates the encapsulation, and a tight seal is formed on just that surface (not over the sample). This silicone seal adheres aggressively, preventing evaporation and lessening the possibility of “Edge Effect”.


The plasticized backing of this film is scored on both ends to allow for two-handed (precise) placement on the plate. Both film and adhesive maintain incredible clarity and are the choice for highly demanding manual and automated processes.

 

Specifications
 
MaterialPET (polyethylene terephthalate)
Adhesive MaterialEncapsulated Silicone; 2.0 Mil
Liner MaterialPolyester
ColorClear
AlphanumericNone
Surface TreatmentNone
CertifiedDNase-/RNase-/Pyrogen-Free
Overall Height2.8 Mil
Overall Thickness2.8 Mil
Adhesive ThicknessEncapsulated Silicone; 2.0 Mil
Top Width3.06 In. | 77.80 mm
Top Length5.56 In. | 141.27 mm
AutoclavableNo
Max Temp.100ºC (212ºF)
Min Temp.-70ºC (158ºF)
 

Item#:
ASADHESIVEPOLFIN28


Hoefer® Chroma Deluxe Dual Cool Vertical Protein Electrophoresis System

 

Ergonomic design offers easier handling and sample introduction.

  • Maximum glass plate size 18 x 16 cm
  • Gels are fully submerged for greater temperature equilibration
  • Produces straight lanes and sharp, well-defined bands
  • Run up to four gels (maximum 112 samples) at one time under identical conditions
  • Accommodates denaturing and native polyacrylamide gels, and the second dimension of 2-D electrophoresis
  • Run gels at uniform temperature from 1 to 45°C
  • A wide array of accessories enables you to tailor gel configurations to your needs 

Hoefer® Chroma Deluxe Complete Unit (SE600X-15-15)

  • Lower Buffer Chamber, 1/ea
  • Upper Buffer Chamber, 1/ea
  • Safety Lid w/High Voltage Leads, 1/ea
  • Heat Exchanger, 1/ea
  • Glass Plates 18 x 16 cm, 6/pk
  • Clamp Assemblies, 16 cm, 4/pk
  • Cams, 6/pk
  • Slotted Gaskets for Upper Buffer Chamber, 2/pk
  • Dual Gel Casting Stand with Leveling Base, 1/ea
  • Bubble Level, 1/ea
  • Laminated Gaskets for Caster, 2/pk
  • Buffer Dam, 1/ea
  • Spacer-Mate Alignment Template, 1/ea
  • Wonder Wedge Plate Separation Tool, 1/ea
  • Gel Seal, 1/ea
  • Combs, 15-well, 1.5 mm thick, 2/pk
  • Spacers, 1.5 mm thick, 2 cm wide, 4pk

Hoefer® Chroma Deluxe Basic Unit (SE600X)

The same as above without the combs and spacers. Order two SE511 combs and two sets of 16 cm long spacers separately.

Specifications
 
Plate Size
(W x H)
7.09 x 6.30, or 7.09 x 3.15 In.
18 x 16 or 18 x 8 cm
Spacers 0.75 mm, 1.0 mm, 1.5 mm
Max. Power1000 V, 500 mA, 50 W
Max. Temp.113ºF | 45°C
Indoor Use 39.2 to 104ºF | 4 to 40°C
HumidityUp to 80%
Dims (D x W x H)5.51 x 12.60 x 11.42 In. | 14 x 32 x 29 cm
Weight16.98 lb | 7.70 kg

Proudly made in the USA! Click here for more made in America products!
 

Item#:
ASHOFRSE600XES

1 x 8 FilmStrips

For Sealing Only One 8-well Row of a 96-well Plate.

Designed for use with either 1×8 strip-well ELISA plates or standard 96-well plates where rows must be selectively protected or accessed. Ideal for COVID-19 testing to seal a row of 8 wells after swabs are deposited into a deep-well plate.
  • Certified DNase- & RNase- free
  • A pliable polypropylene film minimizes aerosols upon removal
  • Each sheet contains 8 strips on a continuous liner for ease of use
  • Apply individually or two-at-a-time
  • Use as a secondary film to seal previously pierced wells or to prevent accidental pipetting
Applications
  • General laboratory use
  • Incubation
  • ELISA
Temp. Range
  • -40°C to +120°C

Proudly made in the USA! Click here for more made in America products!
 
 

Item#:
AS1X8FILMSTRIPS


Accuris MyVolt Mini Power Supply

  • Compact and simple
  • Choose constant 100 V or 200 V output
  • Two pairs of output terminals 

The Accuris MyVolt Mini is a compact electrophoresis power supply ideal for basic, constant voltage applications. Users can select constant 100 V or 200 V output, and the current can reach a maximum of 200 mA.

 

Two sets of output jacks are available to connect up to 2 gel rigs and these small power supplies can securely be stacked for use with additional gel rigs.

Specifications

Output Voltage100V/200V constant
Current OutputMax. 200mA
Max Wattage40W
Output Terminals2 pairs
Weight0.9 lbs / 0.4 kg
Dimensions6.25 x 4.5 x 2.3 in.
16 x 11.5 x 5.7 cm
Electrical100 - 240V, 50-60Hz

 


Item#:
ASPS2100


Hoefer® Tall Standard Dual Cool Vertical Protein Electrophoresis System

 

Taller vertical format accommodates 24 cm long gels for increased resolution.

  • Maximum glass plate size 18 x 24 cm
  • Produce straight lanes and sharp, well-defined bands
  • Run up to four gels (maximum 112 samples) at one time under identical conditions
  • Run gels at uniform temperature from 1 to 45°C
  • Accommodates denaturing and native polyacrylamide gels and 2-D electrophoresis
  • A wide array of accessories allows you to tailor gel configurations to your needs  

Hoefer® Tall Standard Complete Unit (SE660-15-15)

  • Lower Buffer Chamber, 1/ea
  • Upper Buffer Chamber, 1/ea
  • Safety Lid w/High Voltage Leads, 1/ea
  • Heat Exchanger, 1/ea
  • Glass Plates 18 x 24 cm, 6/pk
  • Clamp Assemblies, 8 cm, 4/pk
  • Clamp Assemblies, 16 cm, 4/pk
  • Cams, 6/pk
  • Slotted Gaskets for Upper Buffer Chamber, 2/pk
  • Dual Gel Casting Stand with Leveling Base, 1/ea
  • Bubble Level, 1/ea
  • Laminated Gaskets for Caster, 2/pk
  • Buffer Dam, 1/ea
  • Spacer-Mate-Alignment Template, 1/ea
  • Wonder Wedge™-Plate Separation Tool, 1/ea
  • Gel Seal, 1/ea
  • Combs, 15-well, 1.5 mm thick, 2/pk
  • Spacers, 1.5 mm thick (2 cm wide), 4/pk
  • Lower Buffer Chamber, 1/ea 

Hoefer® Tall Standard Basic Unit (SE660)


The same as above without the combs and spacers. Order two SE511 combs and two sets of 24 cm long spacers separately.

 

Specifications
 
Plate Size
(W x H)
7.09 x 9.45, 7.09 x 6.30, or 7.09 x 3.15 In.
18 x 24, 18 x 16 or 18 x 8 cm
Spacers 0.75 mm, 1.0 mm, 1.5 mm
Max. Power1000 V, 500 mA, 50 W
Max. Temp.113ºF | 45°C
Indoor Use 39.2 to 104ºF | 4 to 40°C
HumidityUp to 80%
Dims (D x W x H)5.51 x 12.60 x 14.57 In. | 14 x 32 x 37 cm
Weight19.40 lb | 8.80 kg

Proudly made in the USA! Click here for more made in America products!
 

Item#:
ASHOFRSE660ES


Hoefer® Standard Dual Cooled Vertical Protein Electrophoresis System

 

Standard vertical format for 18 cm long gels.

  • Maximum glass plate size 18 x 16 cm
  • Produce straight lanes and sharp, well-defined bands
  • Run up to four gels (maximum 112 samples) at one time under identical conditions
  • Run gels at uniform temperature from 1 to 45°C
  • Accommodates denaturing and native polyacrylamide gels and 2-D electrophoresis
  • A wide array of accessories allows you to tailor gel configurations to your needs 

Hoefer® Standard Complete Unit (SE600-15-15)

  • Lower Buffer Chamber, 1/ea
  • Upper Buffer Chamber, 1/ea
  • Safety Lid w/High Voltage Leads, 1/ea
  • Heat Exchanger, 1/ea
  • Glass Plates 18 x 16 cm, 6/pk
  • Clamp Assemblies, 16 cm, 4/pk
  • Cams, 6/pk
  • Slotted Gaskets for Upper Buffer Chamber, 2/pk
  • Dual Gel Casting Stand with Leveling Base, 1/ea
  • Bubble Level, 1/ea
  • Laminated Gaskets for Caster, 2/pk
  • Buffer Dam, 1/ea
  • Spacer-Mate Alignment Template, 1/ea
  • Wonder Wedge Plate Separation Tool, 1/ea
  • Gel Seal, 1/ea
  • Combs, 15-well, 1.5 mm thick, 2/pk
  • Spacers, 1.5 mm thick, 2 cm wide, 4/pk

Hoefer® Standard Basic Unit (SE600)


The same as above without the combs and spacers. Order two SE511 combs and two sets of 16 cm long spacers separately.

 

Specifications
 
Plate Size
(W x H)
7.09 x 6.30, or 7.09 x 3.15 In.
18 x 16 or 18 x 8 cm
Spacers 0.75 mm, 1.0 mm, 1.5 mm
Max. Power1000 V, 500 mA, 50 W
Max. Temp.113ºF | 45°C
Indoor Use 39.2 to 104ºF | 4 to 40°C
HumidityUp to 80%
Dims (D x W x H)5.51 x 12.60 x 11.42 In. | 14 x 32 x 29 cm
Weight15.43 lb | 7.00 kg

Proudly made in the USA! Click here for more made in America products!
 

Item#:
ASHOFRSE600ES

AbsorbMax Black Vinyl Sealing Film

AbsorbMax is a black vinyl sealing film for black microplaes that can be applied to either the top or bottom of the plate.

  • Minimizes background fluorescence and cross talk between plate wells
  • Ideal as a back seal for black plates with clear well bottoms for top reading measurements
  • Absorbs stray light, reducing the background caused by light scatter
  • Protects light sensitive samples from degradation
  • Features a single split liner for easy application

Applications

  • Fluorescence assays
  • Light sensitive sample protection

Temp. Range

  • -40°C to +80°C

Proudly made in the USA! Click here for more made in America products!
 

BrightMax Sealing Film

White, light-reflecting, vinyl sealing film designed for use with white microplates.

  • The light reflective properties on clear-bottom plates maximize the sensitivity of bioluminescence and chemiluminescence assays in bottom-reading luminometers
  • Maximizes intensity and signal-to-noise ratios in luminescence assays
  • May be used as a backing matrix for microscopy of punch-out samples for filter plates (e.g. ELISPOT assays)
  • Features a single split liner for easy application

Applications

  • Luminescence
  • Fluorescence
  • Microscopy
Temp. Range
  • -40°C to +80°C

Proudly made in the USA! Click here for more made in America products!
 

Plate-Sealing Roller

Application: Plate-sealing

Material:
Rubber, metal

The plate-sealing roller features a smooth spinning, thermoplastic elastomer sealing surface with a durable plated metal handle.

  • Reusable, autoclavable
  • Provides a large 4 inch (10.16 cm) wide sealing surface
  • A 10 minute or greater dwell me at room temperature before processing increases the film-to-plate bond

Proudly made in the USA! Click here for more made in America products!
 


Hoefer® Wide-Mini Vertical Protein Electrophoresis System

 

Extra wide format is ideal for quick, high-throughput runs. The SE640 is an extra wide vertical protein electrophoresis unit that accommodates up to 112 samples at one time.

  • Maximum glass plate size 18 x 8 cm
  • Permits rapid screening of up to 112 samples at one time
  • Design permits gels to be surrounded uniformly by buffer so heat dissipation is efficient. 
  • Bands are sharp and lanes are straight
  • Accommodates denaturing or native polyacrylamide gels
  • Run up to four second dimension gels using IEF strips or tube gels

Hoefer® Wide-Mini Complete Unit (SE640-15-15)

  • Lower Buffer Chamber, 1/ea
  • Upper Buffer Chamber, 1/ea
  • Safety Lid with High Voltage Leads, 1/ea
  • Glass Plates 18 x 8 cm, 6/pk
  • 8 cm Clamp Assemblies, 4/pk
  • Cams, 6/pk
  • Dual Casting Stand with Leveling Base, 1/ea
  • Laminated Gaskets for Casting Stand, 2/pk
  • Slotted Gaskets for Upper Buffer Chamber, 2/pk
  • Buffer Dam, 1/ea
  • Spacer-Mate Alignment Template, 1/ea
  • Wonder Wedge Plate Separation Tool, 1/ea
  • Gel Seal, 1/ea
  • Bubble Level, 1/ea
  • Combs, 15-well, 1.5 mm thick, 2/pk
  • 1.5 mm Thick Spacers, 2 cm wide, 4/pk

Hoefer® Wide-Mini Basic Unit (SE640)


The same as above without the combs and spacers. Order two SE511 combs and two sets of 8 cm long spacers separately.

 

Specifications
 
Plate Size (W x H)7.09 x 3.15 In. | 18 x 8 cm
Spacers 0.75 mm, 1.0 mm, 1.5 mm
Max. Power1000 V, 500 mA, and 50 W
Max. Temp.113ºF | 45°C
Indoor Use 39.2 to 104ºF | 4 to 40°C
HumidityUp to 80%
Dims (D x W x H)5.51 x 12.60 x 11.42 In. | 14 x 32 x 29 cm

Proudly made in the USA! Click here for more made in America products!
 

Item#:
ASHOFRSE640ES


For a limited time, purchase any Hoefer® SE260 Mini Gel Unit,

(SE260-10A-10, SE260-10A-15, SE260-10A-75, or SE260B)

& get 5 Hoefer® Mighty Small Precast Gels FREE!

 

When ordering free product, use item number plus “-VF”.

 

Promo valid from 6/10/25 - 7/31/25.
Offer cannot be combined with other promotions, discounts, or institutional pricing. Not valid on prior purchases.
Special price applies only to the specified item; no substitutions.
Allterms and conditionsapply.


Hoefer® Mighty Small II Deluxe Mini Vertical Electrophoresis System

 

Combines the advantages of small format and efficient cooling for rapid screening of proteins and nucleic acids.

  • Maximum glass plate size 10 x 10.5 cm
  • Flexible design, additionally able to accommodate 10 x 8 cm format with optional accessories
  • Supports a wide variety of precast gels
  • Efficient active cooling ensures sharp bands
  • Run up to four gels at one time under identical conditions
  • Quick and easy to assemble
  • Choose from a wide variety of accessories  

Hoefer® Mighty Small II Deluxe Complete Units (SE260-10A)

  • Lower Buffer Chamber, 1/ea
  • Upper Buffer Chamber/Cooling Core, 1/ea
  • Safety Lid with High Voltage Leads, 1/ea
  • Casting Cradle w/Sealing Gasket Set, 1/ea
  • Casting Clamp Assemblies, 2/ea
  • Cams, 4/ea
  • Glass Plates, Rectangular, 10 x 10.5 cm, 10/ea
  • Alumina Plates, Notched, 10 x 10.5 cm, 5/ea
  • Spring Clamps, 4/ea
  • Combs, 10 well, 2/ea
  • T-Spacers, 4/ea
  • Well Locating Decals, 2/ea
  • Gel Seal, 1/ea

Hoefer® Mighty Small II Deluxe Basic Unit (SE260B)

The same as above without the combs, spacers, plates, and caster. For use with precast gels or order two SE211A combs, two sets of 10.5 cm long spacers, 10 x 10.5 cm rectangular glass and notched alumina plates, and a gel caster.

  • Alumina Plates, Notched, 10 x 10.5 cm, 5/pk
  • Spring Clamps, 4/pk
  • Combs, 10 well, 2/pk
  • T-Spacers, 4/pk
  • Well Locating Decals, 2/pk
  • Gel Seal/pk

Hoefer® Mini Electrophoresis and Western Blot Package


Save time and money with a combination package.

  • SE260-10A-75 Mighty Small II Mini Deluxe Vertical Unit
  • TE22 Mighty Small Mini Transfer Tank
  • PS300B 300 V, 500 mA, 90 W Power Supply   

Specifications
 
Plate Size (W x H)3.34 x 4.14 or 3.34 x 3.15 In. | 10 x 10.5 cm or 10 x 8 cm
Spacers0.75 mm, 1.0 mm, 1.5 mm
Max. Power500 V, 500 mA, 12 W
Max. Temp.113ºF | 45°C
Indoor Use39.2 to 104ºC | 4 to 40°C
HumidityUp to 80%
Dims (D x W x H)6.30 x 6.50 x 7.08 In. | 16 x 16.5 x 18 cm
Weight3.30 lb | 1.50 kg

Proudly made in the USA! Click here for more made in America products!
 
 

Item#:
ASHOFRMTYSMDLX
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