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PCR/qPCR

MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more.  If you are looking for any of these things, we can match almost all needs to all cyclers.  If you don’t find it easily contact us at tech@midsci.com and we will find it for you. 

96 or 384 we have it.  Non-Skirted, semi-skirted or full skirted, we have it.  0.1, 0.2, or 0.5 mL we have it.  A1, A12, H12, A24, P24 cuts, we have them.  Sybr© Green or probe alternative, we have it.  

Pro Tip: Colored plastics do not have any impact on DNA amplification.  But when setting up qPCR it is often recommended to use white plastics.  This will limit or prevent fluorescence refraction.  Light refraction can minimize sensitivity and consistency. 

Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips.  This will help optimize and reduce variance in the reactions.  Simple test, see the residual liquid left in tips?  This means some of the taq, dNTPs, sample, etc is left in the tip.  PR1MA tips help reduce that significantly and improve your consistency. 

Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate?  Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler.  Call us we have solutions.

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U of M: Each

Achieves accurate pipetting by allowing the flexibility to adjust the angle of the microplate (45° max. tilt). The stand takes up little benchtop space measuring only 6"L x 4.50"W x 2.50"H.

(Well Orienters are sold separately)

Item#:
WPAJ-1000
Your Price:
91.25
Each
Item#:
ASPCRHALFSKIRT

SureFrame™ Two-Component PCR Plates


SureFrame™ plates are made with a special two material process that creates an ultra-rigid polycarbonate frame, while maintaining the favorable thermal transfer characteristics of thin-walled polypropylene tube wells. This ultra-rigid frame is not only ideal for all automated platforms and plate grippers, but also increases the uniformity of your experiments. Traditional single-material plates have been shown to warp inside thermal cyclers. This deformation of the plates causes increased evaporation in the outer wells of the plate, leading to inconsistent amplification across wells. SureFrame’s polycarbonate frame eliminates warping, for completely uniform results.

  • Two material design for increased rigidity
  • Ideal for automated platforms
  • Reduces evaporation
  • Certified for PCR 
Item#:
ASSUREFRAMETWOP
Item#:
ASPCR96H0P1
Item#:
ASR96SEALMAT
Need a great PCR plastic and at a better price? Try PR1MA. 
 Click here to Order Now!
 
  • Compatible with most robotics, designed to SBS standards.
  • Rigid skirt for gripping by robotic plate handlers.
  • Minimal warp and shrinkage after thermal cycling
  • Compatible with ABI 3730 Automated Sequencers- single cut corner!
Item#:
PCRPLATES2
Please know that the manufacturer has suspended manufacturing this product indefinitely. 
We suggest purchasing PR1MA PCR Plastics.
Contact your rep or email tech@midsci.com for more information.

 
  • Ergonomic Plate Design
    • Easy to see black alphanumeric labeling
    • Cut Corner A12
  • Ideal paring with Caps
    • Flat 8-strip (AVST-01)
    • Domed 8-strip (AVSTC-N)
    • Domed 12-strip (AVSTC-12)
    • Additional Caps
  • Product certified free from RNase, DNase, DNA, PCR inhibitors and endotoxin

Order#

Description

Non-Skirted

 

AVRT-N

Pryme PCR Ergonomic Plate, 96-Well, 0.2 mL, Non-Skirted, Regular Profile, Natural, 25/cs

Semi-Skirted

 

AVRT1

Pryme PCR Ergonomic Plate, 96-Well, 0.2 mL, Semi-skirted, Regular Profile, Natural, 25/cs

Skirted

 

AVSK96

Pryme PCR Ergonomic Plate, 96-Well, 0.1 mL, Skirted, Regular Profile, Natural, 25/cs

Caps

 

AVST-01 

Pryme PCR Ergonomic 8-Strip, Flat Caps, Natural, 125/cs

AVSTC-N

Pryme PCR Ergonomic 8-Strip, Dome Caps, Natural, 125/cs

AVSTC-12

Pryme PCR Ergonomic 12-Strip, Dome Caps, Natural, 80/cs

Please give us a call for a sample!

Item#:
PCRPLATES3

PCR Plates from MIDSCI are designed and developed with maximum mold and manufacturing accuracy.  Use of a low cavity mold and stringent quality control mechanisms ensures you are receiving the highest quality for your research.


With low profile, regular profile, notched corners, and more, we have PCR and qPCR Plates to fit almost every thermal cycler in the market.  Ask us to help you find one for your research today!

Item#:
PCRPLATES6
Please know that the manufacturer has suspended manufacturing this product indefinitely.
We suggest purchasing PR1MA PCR Plastics.
Contact your rep or email tech@midsci.com for more information.


  • Plate Characteristics
    • 96 x 0.2mL, Non-Skirt
    • Regular Profile, Natural
  • Ergonomic Plate Design
    • Easy to see black alphanumeric labeling
  • Highest standards of quality control with pin hole testing
    • Plate to plate and lot to lot consistency
  • Ideal paring with Caps
    • Flat 8-strip (AVT3799)
    • Domed 8-strip (AVT3878)
  • Certified free from DNase/RNase, nucleic acid, PCR-inhibitor, and pyrogen-free 


    Order#

    Description

    Non-Skirt

     

    AVT2619

    MIDSCI PCR Ergonomic Plate, 96-Well, 0.2mL, Non-Skirt, Regular Profile, Natural, 25/cs

    Semi-Skirted

     

    AVT7437

    MIDSCI PCR Ergonomic Plate, 96-Well, 0.2mL, Semi-skirted, Regular Profile, Natural, 25/cs

    AVT3887

    MIDSCI PCR Ergonomic Plate, 96-Well, 0.1mL, Semi-skirted, Regular Profile, White, 25/cs

    Sub-Skirted

     

    AVT3890

    MIDSCI PCR Ergonomic Plate, 96-Well, 0.1mL, Sub-skirted, Low Profile, Natural, 25/cs

    Skirted

     

    AVT2844

    MIDSCI PCR Ergonomic Plate, 96-Well, 0.2mL, Skirted, Regular Profile, Natural, 25/cs

    Caps

     

    AVT3799

    MIDSCI PCR Ergonomic 8-Strip, Flat Caps, Natural, 250/cs

    AVT3878

    MIDSCI PCR Ergonomic 8-Strip, Dome Caps, Natural, 250/cs

    Please give us a call for a sample!

     

Item#:
PCRPLATES5
Item#:
ASPCRMULTIT
Item#:
ASPCR8STQOF
Item#:
ASPCRFULLSKIRT
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