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PCR/qPCR

MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more.  If you are looking for any of these things, we can match almost all needs to all cyclers.  If you don’t find it easily contact us at tech@midsci.com and we will find it for you. 

96 or 384 we have it.  Non-Skirted, semi-skirted or full skirted, we have it.  0.1, 0.2, or 0.5 mL we have it.  A1, A12, H12, A24, P24 cuts, we have them.  Sybr© Green or probe alternative, we have it.  

Pro Tip: Colored plastics do not have any impact on DNA amplification.  But when setting up qPCR it is often recommended to use white plastics.  This will limit or prevent fluorescence refraction.  Light refraction can minimize sensitivity and consistency. 

Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips.  This will help optimize and reduce variance in the reactions.  Simple test, see the residual liquid left in tips?  This means some of the taq, dNTPs, sample, etc is left in the tip.  PR1MA tips help reduce that significantly and improve your consistency. 

Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate?  Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler.  Call us we have solutions.

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For Easy Pipetting and Identification of Samples in Microplates

A thin, plastic card that slips under the well plate, providing sharp, alpha-numeric characters to identify each sample in a 96-, 48-, or 24-well microplate.

No more cross-referencing headaches!

24-Well Orienter

Format: 24-well
U of M: 4/pack

48-Well Orienter

Format: 48-well
U of M: 4/pack

96-Well Orienter

Format: 96-well
U of M: 4/pack

Item#:
WELL-1000
Your Price:
26.75
Each
Pryme is now PR1MA™ Click to learn more!
These tubes are now PR1MA.  New name, new look, same great PCR/qPCR plastics.

  • Dimensional uniformity for superior thermal transfer
  • AVST-01 flat caps are optically clear and ideal for qPCR
  • Certified DNAse/RNAse, nucleic acid, PCR-inhibitor, and pyrogen-FREE

Please give us a call for a sample!

Item#:
PCRTUBES3
Pryme is now PR1MA™ Click to learn more!

    Pryme Ergonomic PCR 8-Strip Tubes
      • Tube Characteristics
        • 0.1mL, low profile
        • Easy open - easy close
      • Extreme Uniformity (EU) Tube Design
        • Uniform wall thickness ensures consistent even heating
        • Thin wall-bottomed tube for rapid heat transfer
      • Pairs with EU âSealing Ring Band❠designed caps
        • Caps are significantly easier to apply and remove -- Repetitive Strain Injury (RSI) Friendly.
        • Significant reduction in evaporation due to a wider sealing area.
      • Certified DNA, DNase, RNA, RNase and pyrogen-free
      Please give us a call for a sample.

    Order #

    Description

    B79601

    Pryme PCR Ergonomic 8-Strip Tubes, 0.1mL, Low Profile, Natural, 120/cs

    B79609

    Pryme PCR Ergonomic 8-Strip Tubes, 0.1mL, Low Profile, White, 120/cs

    B57801

    Pryme PCR Ergonomic 8-Strip, Optical Flat (V2) Caps, Natural, 120/cs

    B79701

    Pryme PCR Ergonomic 8-Strip, Optical Flat (V1) Caps, Natural, 120/cs

    C79701

    Pryme PCR Ergonomic 8-Strip, Semi-Domed (V1) Caps, Natural, 120/cs
Item#:
PCRTUBES8
Pryme is now PR1MA™ Click to learn more!

Please know that the manufacturer has suspended manufacturing this product indefinitely. 
We suggest purchasing PR1MA PCR Plastics.
Contact your rep or email tech@midsci.com for more information.



Thinwalled 0.2ml flat-top tubes with integral cap are suitable for use in all thermal cyclers with a 0.2ml block format.

Item#:
PCRTUBES7
Pryme is now PR1MA™ Click to learn more!

These tubes are now PR1MA:  New name, new look, same great tubes. 
 Click here to Order Now!

Pryme PCR Standard Individual Tubes 0.2 & 0.5 mL

Thinwall flattop or domed capped with attached tubes are available in 0.2 mL and 0.5 mL volumes.   0.2 mL tubes are suitable for use in all thermal cyclers with standard 0.2 mL block formats.  0.5 mL tubes are suitable for use in all thermal cyclers with standard 0.5 mL block formats.  Available in clear tubes, individual colors, and assorted colors.  The thin wall feature accelerates heat transfer, providing shorter cycle times.

Item#:
PCRTUBES5
Domed, Clear, 250/bg
This item has been discontinued and replaced by item 

Please know that the manufacturer has suspended manufacturing this product indefinitely. 
We suggest purchasing PR1MA PCR Plastics.
Contact your rep or email tech@midsci.com for more information.


MIDSCI PCR 8-Strip Caps are compatible with MIDSCI PCR plates. The caps are available as a dome caps (AVT3878) or flat caps (AVT3799).
Specifications of MIDSCI strip caps:

  • Highest standards of quality control
  • Lot to Lot consistency
  • Certified DNase/RNase, nucleic acid, PCR-inhibitor and pyrogen-free
  • See fit chart for plate compatibility
Item#:
AVT3878
Your Price:
42.41
Each
This item is discontinued
Please know that the manufacturer has suspended manufacturing this product indefinitely. 
We suggest purchasing PR1MA PCR Plastics.
Contact your rep or email tech@midsci.com for more information.

PCR Microplate, 96 Well 

  • Standard thermal cycling and qPCR compatible
  • Ultra-thin and consistent wall thickness for precise thermal transfer
  • Made from 99.9% virgin polypropylene
  • Single notch corner
  • 335ul well capacity (working capacity dependent on closure method)
  • Fits standard thermal cyclers, ABI Real Time PCR 7300, 7500, 7900, qPCR and ABI Sequencing
  • Certified DNAse/RNAse and pyrogen-safe
Item#:
ASPRCMICRO96WELL
Colors, 5/pk

PCR Work-Up Rack fits PCR 0.2ml tubes, strip tubes or 96-well plates (including skirted plates). Polypropylene rack and lid click firmly for secure transportation and storage. Available in assorted colors: blue, green, yellow, violet and red.

Item#:
WUR-96A
Your Price:
42.36
Each
Item#:
AS48RECT4P6ML
Item#:
AS384V120UL
96x0.1ml, Sub-Skirted,
  • Plate Characteristics
    • 96 x 0.1mL, Sub-skirted
    • Low Profile (0.1mL), Frosted (low signal to noise ratio
    • Extreme Uniformity (EU) Plate Design
    • Uniform wall thickness ensures consistent even heating
    • Thin wall-bottomed tube for rapid heat transfer
  • Ideal paring with EU Caps with "Sealing Ring Band" Design
    • Easy open - easy close with ergonomic caps: B57801, B79701, C79701
    • Caps are significantly easier to apply and remove -- Repetitive Strain Injury (RSI) Friendly.
    • Significant reduction in evaporation due to a wider sealing area.
  • Certified DNA, DNase, RNA, RNase and pyrogen-free

Please give us a call for a sample!

 

Order#

Description

Non-Skirt

 

B70501

Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, Natural, 25/cs

B70509

Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, White, 25/cs

B50501

Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, Frosted, 25/cs

AB70651

Pryme PCR Plate, 96-Well, 0.1mL, Non-Skirt, Low Profile, Frosted, 25/cs

Semi-Skirted

 

B70651

Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, Natural, 25/cs

B70659

Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, White, 25/cs

B50651

Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, Frosted, 25/cs

B17480

Pryme PCR Plate, 96-Well, 0.1mL, Semi-skirted, Low Profile, Frosted, 25/cs

B17489

Pryme PCR Plate, 96-Well, 0.1mL, Semi-skirted, Low Profile, White, 25/cs

Sub-Skirted

 

ABI7501

Pryme PCR Plate, 96-Well, 0.2mL, Sub-skirted, Regular Profile, Frosted, 25/cs

ABI7509

Pryme PCR Plate, 96-Well, 0.2mL, Sub-skirted, Regular Profile, White, 25/cs

AB19800-EU

Pryme PCR Plate, 96-Well, 0.1mL, Sub-skirted, Low Profile, Frosted, 25/cs

AB19809-EU

Pryme PCR Plate, 96-Well, 0.1mL, Sub-skirted, Low Profile, White, 25/cs

Caps

 

B57801

Pryme PCR Ergonomic 8-Strip, Optical Flat (V2) Caps, Natural, 120/cs

B79701

Pryme PCR Ergonomic 8-Strip, Optical Flat (V1) Caps, Natural, 120/cs

C79701

Pryme PCR Ergonomic 8-Strip, Semi-Domed (V1) Caps, Natural, 120/cs
Item#:
AB19800-EU
Your Price:
117.35
Each
Non-Skirted. Low-Profile,

  • Non-Skirt, low-profile (0.1ml)
  • Cuttable into 16, 24, 32, or 48-well pieces
  • White for optimal qPCR signal/noise ratios
  • Thin-wall for consistent and accurate heat transfer
  • Ultra-low sample evaporation
  • Certified DNAse/RNAse, nucleic acid, PCR-inhibitor, and pyrogen-FREE

 

Closure can be accomplished with B57801 optical indented strip caps.

Item#:
AB70659
Your Price:
164.84
Each
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