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PCR/qPCR
MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more. If you are looking for any of these things, we can match almost all needs to all cyclers. If you don’t find it easily contact us at tech@midsci.com and we will find it for you.
96 or 384 we have it. Non-Skirted, semi-skirted or full skirted, we have it. 0.1, 0.2, or 0.5 mL we have it. A1, A12, H12, A24, P24 cuts, we have them. Sybr© Green or probe alternative, we have it.
Pro Tip: Colored plastics do not have any impact on DNA amplification. But when setting up qPCR it is often recommended to use white plastics. This will limit or prevent fluorescence refraction. Light refraction can minimize sensitivity and consistency.
Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips. This will help optimize and reduce variance in the reactions. Simple test, see the residual liquid left in tips? This means some of the taq, dNTPs, sample, etc is left in the tip. PR1MA tips help reduce that significantly and improve your consistency.
Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate? Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler. Call us we have solutions.
Designed to Facilitate Air and CO2 Exchange.
Designed to Facilitate Air and CO2 Exchange. AeraSeal™ features a hydrophobic, gas permeable membrane with a medical grade, non-cytotoxic adhesive. The porous membrane consists of layers of heat welded rayon fibers.
- Pore size from 10 uM to 50 uM
- Moisture vapor transmission rate = 4,200 g/m²/24 hr
- Easy-to-pierce with serological pipets and pipet tips for direct sample recovery
- Available in roll format for automation: AeraSeal™ Roll-Seal
- Cell culture
- Tissue culture
- Bacterial cultures
- -20°C to +80°C
Proudly made in the USA! Click here for more made in America products!
SealPlate® SealMate™ Rolls
For Fast and Efficient Microplate Sealing. The SealMate SealPlate Starter Kit features a re-usable dispenser and two rolls of perforated film.
The dispenser facilitates the quick and easy dispensing of film from a roll allowing microplates to be sealed three times faster than using sheeted films. Cost-effective replacement rolls are sold separately. Ideal for use under the hood; the SealMate dispenser minimizes film contamination.
- Environmentally friendly; no paper liner to remove and discard; utilizes approximately 72% less material than SealPlate sheeted film
- Each sheet is separated by an adhesive-free, perforated, color-coded green band
- The smooth lower surface of the dispenser can be used to seal the film to the plate
- Dispenser protects unused seals from contamination
- Lower support prop prevents dispensing bill from contacting surfaces to prevent contamination
- Replacement rolls can be easily installed by releasing the locking clasps on the dispenser
- General laboratory use
- Short-term storage
- Incubation
- ELISA
- -40°C to +120°C
Proudly made in the USA! Click here for more made in America products!
General Purpose Sealing Film
For More Secure Seal to Minimize Evaporation.General Purpose polypropylene films offer superior barrier properties to minimize evaporation and prevent well-to-well contamination. Ideal for use with polypropylene plates.
- Certified DNase- & RNase- free
- Features good chemical resistance
- Provides a very secure seal to minimize evaporation, but is slightly harder to remove than SealPlate films
- Less rigid polypropylene film reduces aerosols upon removal
- Specially formulated adhesive minimizes plate residue
- Can be used as a temporary seal while awaiting further processing
- General laboratory use
- Short-term storage
- Incubation
- ELISA
SealPlate® Sealing Film
Industry Standard for Sample Protection.
SealPlate® minimizes evaporation, prevents well-to-well contamination and spillage. Eliminates the “edge effect” which can occur with plate lids (e.g. greater evaporation in the outside plate wells).
- Provides a secure seal and is easy to remove
- Rigid polyester film features good clarity and easy handling
- Can be used as a temporary economical seal while awaiting further processing
- Features two end-tabs with perforations
Applications
- General laboratory use
- Short-term storage
- Incubation
- ELISA
- -40°C to +120°C
Proudly made in the USA! Click here for more made in America products!
eXTReme™ 2 Seal Extra Strong Sealing Film
Application: PCR and robotics
Temp. Range: -20°C to +120°C
Product Info
- Features chamfered corners to eliminate plate overhang for robotics applications and tight storage conditions.
- Certified DNase- & RNase- free
- Next-generation adhesive formula provides better than-ever sample protection with improved evaporation rates
- Standard cut is ideal for use with raised-rim and non-skirted PCR plates
- Large cut is designed for greater plate coverage and easier positioning of the film to the plate
- Large cut is recommended for use with flat-top, semi skirted and full-skirted PCR plates as well as deep well plates
Proudly made in the USA! Click here for more made in America products!
AlumaSeal® II™ Easy-to-pierce Aluminum Foil Seal
Easy-to-pierce, Medical Grade Acrylic Adhesive.
- Certified DNase- & RNase- free
- Excellent chemical resistance (e.g. DMSO)
- Easily pierceable with single or multichannel pipettors and robotic probes
- 1.4mil conformable aluminum foil allows visual verification of the seal’s security around each well
- PCR
- Long-term storage
- Light sensitive assays
- Robotics
- -80°C to +120°C
- End-tab removal before thermal cycling is highly recommended
- Remove diagonally from a corner to prevent tearing
Proudly made in the USA! Click here for more made in America products!
FoilSeal™
- Certified DNase- & RNase- free
- Pierceable with a pipet tip or robotic probe for direct sample recovery
- 2.0mil aluminum foil removes easily without shredding or tearing
- The conformable nature of foil allows for the visual verification of the seal’s security around each well
- Not recommended for long-term storage
- Remove diagonally from a corner to prevent tearing
Proudly made in the USA! Click here for more made in America products!
ThermalSeal® RTS™ Silicone Sealing Film
The silicone adhesive is non-tacky until pressure is applied to the sealing surface which then creates a secure bond around the wells and perimeter of the plate. Also ideal for dry samples (e.g. soil, seeds, insects, etc.) because the non-activated adhesive above the wells remains non-tacky. A roller or sealing paddle is highly recommended for use with this product to ensure consistent and adequate pressure is applied during the sealing process.
- Provides an extremely strong seal to minimize evaporation
- Certified DNase- & RNase- free
- Chemically resistant to DMSO, acetonitrile, and methanol
- Ideal for use with flat-top, raised-rim and non-skirted PCR plates
- High optical clarity and low auto-fluorescence
- Available in roll format for automation: ThermalSeal® RTS™ Roll-Seal
- A complimentary sealing paddle is included in each box
Applications
- qPCR
- Long-term storage
- Protein crystallography
- HPLC
- -80°C to +105°C
Helpful Tips
- Sufficient pressure must be applied to activate the silicone adhesive
- Multiple swipes of the sealing paddle in both directions is highly recommended
- A 10 minute or greater wait period before thermal cycling increases the film-to-plate bond
Proudly made in the USA! Click here for more made in America products!
Click to shop new and improved eXTReme™ 2 Seal Extra Strong Sealing Film
eXTReme™ Seal Extra Strong Sealing Film
eXTReme™ Seal features an extra-strong thick adhesive to minimize evaporation, yet removes easily without leaving residue on the plate.
Features chamfered corners to eliminate plate overhang for robotics applications and tight storage conditions.
- Certified DNase- & RNase- free
- Ideal for use with raised-rim and non-skirted PCR plates
- Available in a larger cut for flat-top semi-skirted and full-skirted PCR plates: eXTReme™ Seal, Large
Application
- PCR
- Robotics
- -20°C to +120°C
Helpful Tips
- Selection of the proper film size will minimize evaporation
- The film should not overhang or ride up the wall of a raised-rim plate
- More complete coverage of skirted flat-top plates will reduce evaporation in the outer wells
- End-tab removal before thermal cycling is highly recommended
Proudly made in the USA! Click here for more made in America products!
ThermalSeal® RT2™ Sealing Film
Optically Transparent, Highly Conformable Adhesive, Polyester Sealing Film.
Designed for use with standard 96-well plates where rows
must be selectively protected or accessed. Available in a smaller cut for raised-rim and non-skirted PCR plates: ThermalSeal® RT2RR
- Certified DNase- & RNase- free
- Ultra-high optical clarity
- Minimizes evaporation and cross-contamination
- Ideal for use with flat-top and hard-shell dual component (e.g. polycarbonate / polypropylene) PCR plates
- Not recommended for removal after thermal cycling
Application
- qPCR
- -40°C to +120°C
Helpful Tips
- Selection of the proper film size will minimize evaporation
- The film should not overhang or ride up the wall of a raised-rim plate
- More complete coverage of skirted flat-top plates will reduce evaporation in the outer wells
- End-tab removal before thermal cycling is highly recommended
Proudly made in the USA! Click here for more made in America products!
1 x 8 FilmStrips™
For Sealing Only One 8-well Row of a 96-well Plate.
Designed for use with either 1×8 strip-well ELISA plates or standard 96-well plates where rows must be selectively protected or accessed. Ideal for COVID-19 testing to seal a row of 8 wells after swabs are deposited into a deep-well plate.- Certified DNase- & RNase- free
- A pliable polypropylene film minimizes aerosols upon removal
- Each sheet contains 8 strips on a continuous liner for ease of use
- Apply individually or two-at-a-time
- Use as a secondary film to seal previously pierced wells or to prevent accidental pipetting
- General laboratory use
- Incubation
- ELISA
Adhesive Sealing Film, Polyolefin
These 2.8 mL, optically clear polyester films are ideally suited for Real Time PCR (qPCR). They feature an inert, ultra-clear, and strong encapsulated silicone adhesive that provides extraordinary performance in real-time PCR applications.
Encapsulated silicone greatly reduces the risk of sample contamination due to exposure to adhesive. Once the film is pressed onto the plate, the raised portion of the well penetrates the encapsulation and a tight seal is formed on just that surface (not over the sample). This silicone seal adheres aggressively and diminishes the possibility of "Edge Effect".
The plasticized backing of this film is scored on both ends to allow for two handed (precise) placement on the plate. Both film and adhesive maintain incredible clarity and are the choice for highly demanding manual and automated processes.
Certified free of RNase, DNase, Pyrogen, and nucleic-acid contamination.
Specifications
| Length | 141.27 mm |
| Length (w/ end tabs removed) | 117.5 mm |
| Width | 77.8 mm |
| Overall Thickness | 2.8 mL |
| Adhesive Thickness | 2.0 mL |
| Material | Polyester |
| Adhesive | Encapsulated Silicone |
| Max. Temperature | 120 °C |
| Min. Temperature | -40 °C |
| Primary Usages | Real Time PCR (qPCR), PCR |
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