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PCR/qPCR
MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more. If you are looking for any of these things, we can match almost all needs to all cyclers. If you don’t find it easily contact us at tech@midsci.com and we will find it for you.
96 or 384 we have it. Non-Skirted, semi-skirted or full skirted, we have it. 0.1, 0.2, or 0.5 mL we have it. A1, A12, H12, A24, P24 cuts, we have them. Sybr© Green or probe alternative, we have it.
Pro Tip: Colored plastics do not have any impact on DNA amplification. But when setting up qPCR it is often recommended to use white plastics. This will limit or prevent fluorescence refraction. Light refraction can minimize sensitivity and consistency.
Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips. This will help optimize and reduce variance in the reactions. Simple test, see the residual liquid left in tips? This means some of the taq, dNTPs, sample, etc is left in the tip. PR1MA tips help reduce that significantly and improve your consistency.
Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate? Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler. Call us we have solutions.
ThermalSeal® 2™ and ThermalSeal® Advanced Sealing Film
ThermalSeal® 2™ and ThermalSeal® Advanced feature a soft, extra thick, conformable adhesive that forms a very strong seal around raised wells to minimize evaporation and cross-contamination.
- Certified DNase- & RNase- free
- Ideal for use with flat-top, semi-skirted and full-skirted PCR plates
- Compatible with hard-shell dual component (e.g. polycarbonate/polypropylene) PCR plates
- Large cut allows for easier positioning of the film to the plate via edge of plate alignment vs. centering
- Available in a smaller cut for raised-rim and non-skirted PCR plates: ThermalSeal® Advanced
Application
- PCR
Temp. Range
- -40°C to +120°C
Helpful Tips
- Selection of the proper film size will minimize evaporation
- The film should not overhang or ride up the wall of a raised-rim plate
- More complete coverage of skirted flat-top plates will reduce evaporation in the outer wells
- End-tab removal before thermal cycling is highly recommended
Proudly made in the USA! Click here for more made in America products!
Zone-Free™ Multilayer Film
Zone-Free™ is a multilayer film consisting of a top pierceable clear polyethylene film and a white polypropylene sublayer with 96 circular areas void of adhesive.
Application: Short-term storage and robotics
Temp. Range: -40°C to +90°C
Proudly made in the USA! Click here for more made in America products!
The white sublayer aids in the alignment of the film to the plate; alignment of opposing corner wells ensures that all 96 wells will be properly aligned. The adhesive-free zones are 9mm on center to facilitate multi-channel pipetting without adhesive fouling of pipet tips or robotic probes.
- Pierceable
- Offers excellent chemical resistance
- Samples have minimal contact with adhesive due to the adhesive-free zones
Proudly made in the USA! Click here for more made in America products!
ThinSeal™ Sealing Film
Ultra-thin, Semi-pierceable Sealing Film. Leaves minimal residue.
- Clear polyester film usable with polystyrene or polypropylene plates
- Marginally pierceable by single-channel pipets and probes
- Reduces evaporation, cross-contamination and spillage
- Removes easily and cleanly while reducing aerosols
Temp. Range: -40°C to 120°C
Proudly made in the USA! Click here for more made in America products!
Item#:
ASTHINSEALSFI
ThermalSeal® Sealing Film
ThermalSeal® features a moderate strength adhesive for ease of removal.- Certified DNase- & RNase- free
- Only recommended for structurally rigid polypropylene PCR plates that don’t warp
- A less rigid polypropylene film that minimizes aerosols upon removal
- Features good chemical resistance
Application
- PCR
- General storage
Temp. Range
- -40°C to +120°C
Proudly made in the USA! Click here for more made in America products!
X-Pierce™ Sealing Film
For Re-sealability and Easy Access Without Pipet Tip Coring.X-Pierce™ film is designed for short-term processing and storage of samples that require multiple access to wells by pipet tips or robotic probes. A pre-cut “X” over each well creates bendable flaps that provide sample access without coring or adhesive fouling of tips or probes. Ideal for COVID-19 sample collection in deep-well plates using swabs.
- Pierceable
- Printed circles aid in the alignment of the film to the plate
- Printed alphanumerics identify each well of a 96-well plate
- Reseals after piercing for continued sample protection
Helpful Tips
- To help position the film to the plate, align wells A-1 and H-12
Applications
- Short-term processing and storage at ambient temperatures
- Robotics
Temp. Range
- -40°C to +90°C
Proudly made in the USA! Click here for more made in America products!
AeraSeal™ SealMate™ Microplate Sealing Film Rolls
Starter Kits and Refills for Fast and Efficient Microplate Sealing.
The AeraSeal™ SealMate™ Starter Kit features a re-usable dispenser and two rolls of perforated film.
The dispenser facilitates the quick and easy dispensing of film from a roll allowing microplates to be sealed three times faster than using sheeted films. Cost-effective replacement rolls are sold separately. Ideal for use under the hood; the SealMate™ dispenser minimizes film contamination.
The dispenser facilitates the quick and easy dispensing of film from a roll allowing microplates to be sealed three times faster than using sheeted films. Cost-effective replacement rolls are sold separately. Ideal for use under the hood; the SealMate™ dispenser minimizes film contamination.
- Environmentally friendly; no paper liner to remove and discard; utilizes approximately 59% less material than AeraSeal™ sheeted film
- Each sheet is separated by an adhesive-free, perforated, color-coded red band
- The smooth lower surface of the dispenser can be used to seal the film to the plate
- Dispenser protects unused seals from contamination
- Lower support prop prevents dispensing bill from contacting surfaces to prevent contamination
- Replacement rolls can be easily installed by releasing the locking clasps on the dispenser
- Designed to facilitate air and CO2 exchange
- Pore size from 10 uM to 50 uM
- Moisture vapor transmission rate = 4,200g/m²/24hr
- Easy-to-pierce with serological pipets and pipet tips for direct sample recovery
Applications
- Cell culture
- Tissue culture
- Bacterial cultures
Temp. Range
- -20°C to +80°C
Proudly made in the USA! Click here for more made in America products!
ValueSeal™ Sealing Film
The most economical film for general laboratory use.ValueSeal™ features smaller dimensions and a single non-perforated end-tab to provide a more cost-effective alternative to protect samples from evaporation, external contamination, dust and particulates.
- Provides a secure seal and is easy to remove
- Rigid polyester film features good clarity and easy handling
- Can be used as a temporary seal while awaiting further processing
- General laboratory use
- Short-term storage
- Incubation
- ELISA
TiterSeal™ Sealing Film
Sample protection you can count on. Minimizes evaporation, prevents well-to-well contamination and spillage.
- Eliminates the “edge effect” which can occur with plate lids (e.g. greater evaporation in the outside plate wells)
- Provides a secure seal and is easy to remove
- Rigid polyester film features good clarity and easy handling
- Can be used as a temporary economical seal while awaiting further processing
- Features two end-tabs with perforations
Applications
- General laboratory use
- Short-term storage
- Incubation
- ELISA
SealPlate® ColorTab™ Sealing Film
For Sample Identification & Plate Labeling.
An inscribed color-coded end-tab can be removed and pasted
into a laboratory notebook while the other remains on the plate for
identification. The film sheet can be removed from the plate without disturbing
the identification label due to the finely perforated end-tabs. Choose from 5
different individual colors or the assortment pack.
- Provides a secure seal and is easy to remove
- Rigid polyester film features good clarity and easy handling
- Features two end-tabs with perforations
Applications
- General laboratory use
- Short-term storage
- Incubation
- ELISA
Temp. Range
- -40°C to +120°C
Proudly made in the USA! Click here for more made in America products!
eXTReme™ FoilSeal™
eXTReme™ FoilSeal™ is an aluminum foil seal that offers excellent chemical resistance and superior performance in ultra-low temperatures.Application: Long-term storage, light sensitive assays and robotics
Temp. Range: -80°C to +120°C
The smaller dimensions and chamfered corners result in no plate overhang for robotics applications; universal fit for use with all plate types (e.g. raised-rim and non-skirted). Or select our larger cut for greater plate coverage: eXTReme™ FoilSeal™, Large
- Certified DNase- & RNase- free
- Recommended for -80°C long-term storage
- Excellent chemical resistance (e.g. DMSO)
- Specially formulated adhesive provides an excellent seal to minimize evaporation and well-to-well contamination
- 1.9 mil thick aluminum foil removes easily without tearing and allows for visual verification of the seal’s security around each well
- Pierceable with a pipet tip for direct sample recovery
- Not recommended for PCR
Helpful Tips
- Remove diagonally from a corner to prevent tearing
- Selection of the proper film size will minimize evaporation
- More complete coverage of the plate will reduce evaporation in the outer wells
Proudly made in the USA! Click here for more made in America products!
EZcap™ PCR FilmStrips
An economical and more user-friendly alternative to PCR strip-caps; no sore thumbs guaranteed!
Application: PCR and short-term storage
Temp. Range: -20°C to +120°C
Proudly made in the USA! Click here for more made in America products!
Precision cut to a smaller dimension for sealing one PCR 8-tube strip or a single row of a 96-well plate whenever rows must be selectively protected or accessed. FilmStrips remove easily with no splash back as can occur with strip-caps.
- Certified DNase- & RNase- free
- Features an extra strong, thick adhesive to minimize evaporation
- Each sheet contains 8 strips on a continuous liner
Helpful Tips
- A non-locking workstation is recommended for sample prep (e.g. 96-well round-boom plate)
- Removal of the end-tabs at the perforations is recommended before thermal cycling
- A final sealing step is highly recommended in the thermal cycler because 8-tube strips flex during handling
Proudly made in the USA! Click here for more made in America products!
AlumaSeal® 384™ and 96™ Aluminum Foil Seal
Easy-to-pierce Aluminum Foil Seal for Direct Sample Recovery.
AlumaSeal® 384™ provides a secure seal for 384-well plates to minimize evaporation, reduce cross-contamination and prevent spillage.AlumaSeal® 96™ provides a secure seal for 96-well raised-rim and non-skirted PCR plates to minimize evaporation, reduce cross-contamination and prevent spillage.
- Certified DNase- & RNase- free
- Ideal for use with 384-well and deep-well plates (or select AlumaSeal® 96™ for smaller cut for raised-rim and non-skirted PCR plates.)
- Features one end-tab with no perforation
- Pierceable with a pipet tip or robotic probe for direct sample recovery
- Large cut allows for easier positioning of the foil to the plate via edge of plate alignment vs. centering
- Available in roll format for automation: AlumaSeal® Roll-Seal, 80 mm x 100 mm
- PCR
- Long-term storage
- Light sensitive assays
- Robotics
- -80°C to +120°C
- End-tab removal before thermal cycling is highly recommended
- Remove diagonally from a corner to prevent tearing
- Selection of the proper film size will minimize evaporation
- The film should not overhang or ride up the wall of a raised-rim plate
- More complete coverage of skirted flat-top plates will reduce evaporation in the outer wells
Proudly made in the USA! Click here for more made in America products!
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