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PCR/qPCR
MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more. If you are looking for any of these things, we can match almost all needs to all cyclers. If you don’t find it easily contact us at tech@midsci.com and we will find it for you.
96 or 384 we have it. Non-Skirted, semi-skirted or full skirted, we have it. 0.1, 0.2, or 0.5 mL we have it. A1, A12, H12, A24, P24 cuts, we have them. Sybr© Green or probe alternative, we have it.
Pro Tip: Colored plastics do not have any impact on DNA amplification. But when setting up qPCR it is often recommended to use white plastics. This will limit or prevent fluorescence refraction. Light refraction can minimize sensitivity and consistency.
Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips. This will help optimize and reduce variance in the reactions. Simple test, see the residual liquid left in tips? This means some of the taq, dNTPs, sample, etc is left in the tip. PR1MA tips help reduce that significantly and improve your consistency.
Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate? Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler. Call us we have solutions.
PRIME Base Thermal Cycler
- Colour touchscreen for fast program setup.
- Temp Range 4°C to 100°C
- Fast ramp rate of up to 3.4°C/sec
- Can be upgraded for gradient cycling
- Data transfer via USB
- 4 year warranty
This full sized thermal cycler delivers both high performance and high throughput to provide maximum flexibility when processing a large number of samples in parallel.
User friendly programming is achieved via a colour touchscreen and intuitive software that is standardised across the entire Techne thermal cycler range. A USB port enables the transfer of programmes between instruments and temperature logs to your PC. A real time graphical display provides an instant visualisation of the program status.
Gradient cycling can be achieved directly using the PRIME G instrument or by upgrading the PRIME BASE unit. This enables the laboratory to manage changing cycling requirements in a cost effective manner.
The Techne PRIME cycler range is one of the most affordable full sized thermal cyclers on the market and is backed up by a 4 year warranty.
Technical Specification
Sample capacity: 0.2ml |
96 |
Sample capacity: 0.5ml |
60 |
Sample capacity: 384 well |
Yes |
Maximum heating rate |
3.4°C/s |
Block Temp Range |
4 °C to 100°C |
Block uniformity at 50°C |
<±0.3°C |
Temperature accuracy at 50°C |
<±0.2°C |
Gradient |
Can be gradient enabled |
Type |
8 peltier element/block |
Maximum cooling rate |
2.6°C/s |
Selectable heated lid temperature |
100 °C to 115°C or off |
Heated lid pressure |
Adjustable, dependent on consumables |
Program interface |
5.7" VGA colour touchscreen with graphical display |
Maximum number of programs stored |
1000 |
Data transfer |
USB port |
Auto re-start on power failure |
Yes |
Dimensions (L x W x H) |
420 x 240 x 240mm |
Weight |
9.4kg |
Voltage |
100-230V, 50-60Hz |
Power |
450W |
Order # |
Description |
|
Prime Base Thermal Cycler (96x0.2ml) |
||
Prime Base Thermal Cycler (60x0.5ml) |
||
Prime Base Thermal Cycler (384-Well) |
||
Prime USB Gradient Upgrade |
||
Prime G Gradient Thermal Cycler (96x0.2ml) |
||
Prime G Gradient Thermal Cycler (60x0.5ml) |
||
Prime G Gradient Thermal Cycler (384-Well) |
||
Sample capacity: 0.2ml |
96 |
Sample capacity: 0.5ml |
60 |
Sample capacity: 384 well |
Yes |
Maximum heating rate |
3.4°C/s |
Block temperature range |
4°C to 100°C |
Block uniformity at 50°C |
<±0.3°C |
Temperature accuracy at 50°C |
<±0.2°C |
Gradient Range |
30°C to 80°C |
Maximum Gradient |
30°C (16°C for 384) |
Minimum Gradient |
1°C |
Type |
8 peltier element/block |
Maximum cooling rate |
2.6°C/s |
Selectable heated lid temperature |
100 °C to 115°C or off |
Heated lid pressure |
Adjustable, dependent on consumables |
Program interface |
5.7" VGA colour touchscreen with graphical display |
Maximum number of programs stored |
1000 |
Data transfer |
USB port |
Auto re-start on power failure |
Yes |
Dimensions (L x W x H) |
420 x 240 x 240mm |
Weight |
9.4kg |
Voltage |
100-230V, 50-60Hz |
Power |
450W |
Order # |
Description |
PRIME02 |
Prime Base Thermal Cycler (96x0.2ml) |
PRIME05 |
Prime Base Thermal Cycler (60x0.5ml) |
PRIME384 |
Prime Base Thermal Cycler (384-Well) |
PRIMEXUSB |
Prime USB Gradient Upgrade |
PRIMEG02 |
Prime G Gradient Thermal Cycler (96x0.2ml) |
PRIMEG05 |
Prime G Gradient Thermal Cycler (60x0.5ml) |
PRIMEG384 |
Prime G Gradient Thermal Cycler (384-Well) |
- Fast ramping - up to 5°C/second
- Intuitive graphical programming
- Lightweight and compact
- Interchangeable blocks - 24 x 0.2 ml and 18 x 0.5 ml
- Economical - half the price of a full size unit
- 18 month warranty
| Product Information | ||
| Labnet's MultiGene Mini is a compact and lightweight thermal cycler, but don't let its small size fool you. This cycler is packed with features typically found only on larger thermal cyclers. | ||
| Excellent accuracy and uniformity combine with fast ramping rates to provide quality results. An algorithm calculates sample temperature, based on Vol, to control heating and cooling of the block. This means that samples reach programmed temperatures quickly, without any overshoot or lagging. Powerful Peltier units provide ramping rates as fast as 5°C/second. | ||
| Programming the MultiGene Mini is simple and intuitive. The control pad combines function keys, a key pad and arrow keys for easy navigation and entering of parameters. The large graphical display is easy to read. In addition to the standard parameters of time and temperature, the software also allows for successive time and temperature increments and decrements (for touchdown amplification and auto-extension), auto-restart after a power failure, end of cycling elongation steps and extended soaks at 4°C. One hundred programs, each with up to nine segments, can be stored in memory. | ||
| The MultiGene Mini is available with either a 24 x 0.2 ml tube block or 18 x 0.5 ml tube block. Blocks are easily interchanged. Units come standard with an adjustable heated lid. The lid features a slip gear to provide proper compression to prevent sample evaporation without deforming the tubes. | ||
| Product Specifications | ||
| Capacity | ||
| TC020-24 | 24 x 0.2 mL tubes | |
| TC050-18 | 18 x 0.5 mL tubes | |
| Programmable temp. range | 4° to 99°C | |
| Temp. accuracy/uniformity | ±0.3°C/±0.5°C at 55°C | |
| Max. heating/cooling rate | 5°C/4°C per second | |
| Heating/cooling method | Peltier | |
| Heated lid temperature | 105°C | |
| Program memory | 100 programs | |
| Max. cycles/segments | 99 cycles/9 segments | |
| Max. hold time/step | 99 minutes 59 seconds | |
| Dimensions (WxDxH) | 8.6 x 11.2 x 7 in / 21.8 x 28.5 x 17.8 cm | |
| Weight | 7.1 lb/3.2 kg | |
| Electrical | 120V/230V, 50/60 Hz | |
IBI DEPC-Treated, Nuclease-Free Water is ideally suited for all applications in molecular biology lab including PCR, RT-PCR, restriction enzyme
assays, modifying enzyme assays, transfection, cloning, transformation, and specifically for all RNA assays.
IBI DEPC-Treated Water is manufactured under stringent conditions. The purification process for this product includes continuous
deionization, reverse osmosis, UV-treatment, 0.1µm filtration, followed by steam sterilization in an autoclave. The product is re-autoclaved
to ensure the breakdown of DEPC and release of carbon dioxide that may cause the pH to shift.
IB42200 |
DEPC Treated Water, nuclease free, 125mL |
IB42201 |
DEPC Treated Water, nuclease free, 24 x 125mL |
IB42202 |
DEPC Treated Water, nuclease free, 48 x 125 mL |
IB42210 |
DEPC Treated Water, nuclease free, 500 mL |
IB42211 |
DEPC Treated Water, nuclease free, 10 x 500 mL |
IB42212 |
DEPC Treated Water, nuclease free, 20 x 500 mL |
IB42220 |
DEPC Treated Water, nuclease free, 1L |
IB42221 |
DEPC Treated Water, nuclease free, 6 x 1L |
IB42222 |
DEPC Treated Water, nuclease free, 12 x 1L |
IB42230 |
DEPC Treated Water, nuclease free, 2L |
IB42231 |
DEPC Treated Water, nuclease free, 6 x 2L |
IB42240 |
DEPC Treated Water, nuclease free, 10L |
- Tracks the migration progression of your sample during polyacrylamide electrophoresis
- Loading dye migrates independently of the samples, making it easier to estimate the migration of proteins
- DNase/RNase/Protease free
Qamp Mini Thermal Cycler
- Compact in Size: 102 x 136 x 104 mm
- Preprogramed Chip: with unlimited applications
- Touch Start: One Button to Go!
- LCD Display: for customers to define status easily!
- Portable: Only 1 kg
Specifications:
| Sample Number | 8 (2 x 4 wells) |
| Temperature Range | 4°C - 99°C |
| Max Heating Rate (°C/sec.) | 4.6°C |
| Max Cooling Rate (°C/sec.) | 3.4°C |
| Temperature Accuracy | ± 0.4°C |
| Temperature Uniformity Across Block | ± 0.4°C |
| Max No. of Cycles | unlimited |
| Max No. of Steps | unlimited |
| Display | LCD |
| Heated Lid | 105°C Fixed (pre-heat to 60°C) |
| Dimensions (L x W x H) | 10 x 13 x 10 cm |
| Weight | 1 Kg |
| Power | VAC 100-240, 50/60 Hz, 120 W |
This item has been discontinued by the manufacturer.
See the new and improved Labnet™ 1001 Mini Plate Spinner Centrifuge for a great alternative.
Labnet™ Mini Plate Spinner Centrifuge
- Quickly spin down droplets and condensation
- Use before and after thermal cycling to increase PCR yield
- Accepts skirted, non-skirted and all standard PCR plates
- Less than 1/4 the size of most plate centrifuges
- 1 year warranty
Mini Plate Spinner Adapter Supports:
- 8 x 0.2 mL PCR Strips
- 12 x 0.2 mL PCR Strips
- 0.2 mL PCR Tubes
- 48 Well PCR Plates
Specifications
| Capacity | 2 PCR Plates |
| Rotor Speed | 2500 rpm |
| G Force | 500 x g |
| Rotor | Fixed Vertically |
| Dims (W x D x H) | 7.5 x 8.2 x 7.2 In. | 19 x 21 x 18.3 cm |
| Weight | 6 lbs | 3 kg |
| Electrical | 120V or 230V, 50-60 Hz |
Gel Cutting Tips
Our Gel Cutting Tips are specially designed to provide an easy, efficient solution for removing bands from agarose gel. Made from high-quality, non-pyrogenic polypropylene, these tips are ideal for applications where contamination-free performance is essential.
- Effortless Band Removal: Perfect for safely extracting bands from agarose gels without damage.
- One-Handed Operation: Designed for ease of use, allowing you to operate with just one hand for faster, more efficient workflows.
- Push Button Gel Release: The convenient push-button design ensures smooth gel band release with minimal effort.
- Ejector Button for Tip Release: Easily eject the tip with a quick press to maintain a clean, contamination-free environment.
- Avoid Cross-Contamination: Features a secure and precise fit to minimize the risk of cross-contamination.
- Universal Compatibility: Compatible with standard 1,000 µL pipettors for seamless integration into your lab setup.
- Non-Pyrogenic & RNase-/DNase-Free: Ensures that your experiments are free from unwanted contamination, preserving the integrity of your samples.
- Non-Sterile: Intended for use in controlled environments where sterilization is not required.
Specifications
| Item # | TGL-1165 |
| Packaging Format | 250 Tips/Pk, 10 Packs/Cs |
| Sterile | No |
| Autoclavable | Yes |
| Color | Clear |
These Rotocycler strips are perfectly designed to perform on Rotor-Gene instruments. Tube strips are packaged separately from cap strips. The frosted extensions on caps not only make them more efficient and secure during handling but also offer a convenient area for labeling. For individual use, tube and cap strips can easily be separated and used as individual units.
Double sided water cooled vertical system, 10cm x 10cm
- Heavy duty upper buffer chamber with cooling port connections.
- Lower buffer chamber.
- Interlock safety lid with attached leads.
- Casting base.
- Two-pack each of plain & notched glass plates.
- One acrylic blocker plate (used when running only one gel).
- Ttwo 10-well, 0.8mm thick combs
- Four 0.8mm thick side spacers.
- System Accessories
- Choose amoung these accessories to customize each system for your specific needs.
- Alumina cooling plate.
- For use with cooling systems.
- This plate can be sandwiched between the gel and the inner buffer chamber to improve overall temperature uniformity.
- Casting base.
- For quick, efficient and leak-proof casting.
- Multiple gradient caster.
Extra Wide Large Format Gel System, 23.5cmW x 40cm
Extra wide large format gel system, 23.5cmW x 40cmL gel size with buffer exchange ports, UVT gel tray with fluorescent ruler and 12 tray slots, (2) 25 teeth combs, 1.5 mm thickness and (2) 50 teeth combs, 1.5 mm thickness.
System Description
For long run lengths, maximum resolution, and really large sample capacity, look no further than the Galileo 2340 device. This unit also features the Galileo ExpressCast system for quick tape-less gel pouring.
The 2340 is our maximum capacity system. With a maximum run length of 25cm, the unit can provide very high resolution of complex analytes. Or, fitted with maximum capacity combs in all of the tray's 12 comb slots, the unit can handle up to 600 samples. The 2340 features the Galileo ExpressCast gel casting system for quick, tape-free gel casting and includes a built-in bubble level and leveling screws for convenient level adjustment. You may choose from a wide variety of comb options to customize your order.
System Features
The Galileo 2340 complete system includes these features:
- Heavy duty lower buffer chamber
- Interlock safety lid with attached leads
- End gated 23cm wide x 40cm long ExpressCast™ UV-transparent gel tray with built-in fluorescent ruler and 12 comb slots.
- Two 1.5mm x 25 tooth combs
- Two 1.5mm x 50 tooth combs
MaGELin Mini Protein System
- For 10 cm x 10 cm cast-your-own polyacrylamide gels
- Comes with dual casting system
- 3 sets of glass plates and 2 sets of 0.8 mm thickness spacers.
- Buffer tank, dual gel capture, vented lid and power cords included
The IB94000 MaGELin Mini protein system comes complete with everything you need to pour 10 cm x 10 cm cast-your-own gels. This highly versatile system will also accommodate the IB95000 Western Transfer module so when you’re not running gels the unit can be used as a dedicated Western transfer system.
The IB94000 comes complete with:
- Dual gel capture device for running two gels simultaneously.
- Dual gel casting system that allows casting outside of the running unit.
- Three sets of glass plates for pouring and running gels
- Two sets of special 0.8 mm side spacers to seal the gel sandwich during pouring
- Buffer tank
- Vented lid
- Power cords.
- Two 0.8 mm x 12-tooth combs.
The IB94000 utilizes a unique spacer and casting system to make it easy and fast to cast your own gels. The special side spacers are made specifically to accommodate the thickness of each glass slide that comes with the unit. These side spacers will produce a 0.8 mm thick gel. Once the glass plates have been assembled using the side spacers, they can now be inserted into the gel casting unit. This unit accommodates two sets of glass plates simultaneously. The bottom of the gel casting unit contains a very soft gel-like gasket that will seal the bottom of the gel sandwich. Once the gel sandwich is placed inside the casting unit, place the lid of the casting unit on top and press gently to fasten using the included thumb screws. Once fastened the lid will provide the correct amount of down pressure on the gel sandwich to seal the bottom and hold them in place. The polyacrylamide solution can now be introduced through the opening in the casting fixture lid.
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