PCR/qPCR

MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more.  If you are looking for any of these things, we can match almost all needs to all cyclers.  If you don’t find it easily contact us at tech@midsci.com and we will find it for you. 

96 or 384 we have it.  Non-Skirted, semi-skirted or full skirted, we have it.  0.1, 0.2, or 0.5 mL we have it.  A1, A12, H12, A24, P24 cuts, we have them.  Sybr© Green or probe alternative, we have it.  

Pro Tip: Colored plastics do not have any impact on DNA amplification.  But when setting up qPCR it is often recommended to use white plastics.  This will limit or prevent fluorescence refraction.  Light refraction can minimize sensitivity and consistency. 

Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips.  This will help optimize and reduce variance in the reactions.  Simple test, see the residual liquid left in tips?  This means some of the taq, dNTPs, sample, etc is left in the tip.  PR1MA tips help reduce that significantly and improve your consistency. 

Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate?  Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler.  Call us we have solutions.

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PR1MA T4 Gene 32 Protein

 

PR1MA T4 Gene 32 Protein (T4 gp32) is a single-stranded DNA binding (ssDNA) protein required for E. coli bacteriophage T4 replication. It binds and stabilizes ssDNA structures which facilitates electron microscopic examination, and has also been shown to improve restriction digests, improve T4 DNA polymerase activity, and increase the yield of PCR reactions, including those with long amplicons. 

  • Optimal temperature: 37°C
  • Heat inactivation: 65°C for 20 minutes
  • Storage temperature: -20°C
  • 10X T4 gp32 reaction buffer included

Buffer composition

  • 50% glycerol
  • 50 mM Tris-HCl
  • 50 mM KCl
  • 1 mM DTT
  • 0.1  mM EDTA
  • 0.1% Tween-20
  • pH = 7.5 

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established. 
 

Proudly made in the USA! Click here for more made in America products!
 


PR1MA Reverse Transcriptase

 

PR1MA reverse transcriptase (RT) is an RNA-dependent DNA polymerase that can be used for complementary DNA (cDNA) synthesis from an RNA template and is ideal for use in molecular amplification assays. PR1MA RT is a robust enzyme that works in a broad range of temperatures (40 - 72°C) and has RNase H activity.

  • Optimal temperature: 55°C
  • Heat inactivation: 75°C for 20 minutes
  • Glycerol-free buffer available
  • Storage temperature: -20°C (standard buffer)
  • 10X Isothermal buffer included

Standard Buffer Composition

  • 50% glycerol
  • 10 mM Tris-HCl
  • 100 mM KCl
  • 1 mM DTT
  • 0.1  mM EDTA
  • pH = 7.5

Important note: Please be sure to use the buffer provided with this product to ensure optimal results.

 

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.   
 

Proudly made in the USA! Click here for more made in America products!
 


PR1MA Reverse Transcriptase, RNase H -

 

PR1MA reverse transcriptase (RT) is an RNA-dependent DNA polymerase ideal for use in RT-PCR and first-strand synthesis of complementary DNA (cDNA) for generation of cDNA libraries from single-stranded RNA, DNA, or RNA:DNA hybrids.

  • Decreased RNase H activity enables longer cDNA synthesis (>5 kb).
  • Lacks 3’ to 5’ exonuclease activity
  • Optimal temperature at 42°C
  • Temperature range: 40 to 50°C
  • Heat inactivation: 70°C for 20 minutes
  • Storage temperature: -20°C
  • 10X reaction buffer included 

Buffer composition

  • 50% glycerol
  • 50 mM Tris-HCl
  • 50 mM KCl
  • 1 mM DTT
  • 0.1  mM EDTA
  • pH = 7.5

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.

 

Proudly made in the USA! Click here for more made in America products!
 


2025 LabValues Promotion!
For a limited time enjoy discounted pricing on the
AccuSeal™ Semi-Automated Plate Sealer (PS1000)!

 

See flyer for more details.

 

Special price applies only to the specified item; no substitutions.

Cannot be combined with other discounts or pricing. Not valid on prior purchases.

Promo is valid 01/06/2025 – 06/30/2025 or while supplies last.

All terms and conditions apply.


AccuSeal Semi-Automated Plate Sealer

 

AccuSeal Semi Automated Plate Sealers from MIDSCI are engineered and constructed by Labnet International, Inc to maximize PCR, assay, and storage applications with a user-friendly panel and automated sealing options.


Great emphasis has been placed on incorporating a wide range of standard heights to deep well microplates with appropriate adaptors to efficiently fit the plates for consistent temperature regulation. The AccuSeal sealer is optimized for simple digital control and operation, user-friendly versatility and convenience as well as safety.

Controls and Operation

  • Intuitive layout permits optimal sealing time, temperature and settings
  • Includes a "No Heat" setting for pressure sealing applications

Versatility and Convenience

  • Adaptors specific for a PCR plate or an assay plate accommodate a variety of fits
  • Automatically adjusts heat for different height plates
  • Provides uniform pressure for a quality seal with a special non-stick coating to prevent messy cleanup.

Safety

  • Motorized drawer features safety switches and prevents injury to the user.

Specifications
 
Dimensions6.75 x 12.75 x 14.25 in.
Weight24 Lbs
Sealing TemperatureOFF, 100 to 190 °C
Temperature Accuracy+/- 1.00 °C
Temperature Uniformity+/- 1.00 °C
Sealing Time0.5 to 10 sec
Compatible Plate MaterialsPP (Polypropylene)
PS (Polystyrene)
PE (Polyethylene)
Compatible Plate TypesStandard Assay Plates
Deep-well Storage Plates
PCR Plates: skirted, semi-skirted, non-skirted
Maximum Plate Height45 mm
Compatible Sealing Film TypesFoil-polypropylene laminate
Clear polyester-polypropylene laminate
Clear polymer
Thin clear polymer
Foil-laminate
Foil

Adapters 
 
PS1000-PCRAccuSeal PCR plate adapter for sealing PCR and 384 well plates
PS1000-ADAPTAccuSeal Assay plate adapter for sealing assay plates

Sealing Film

PS1000-150AccuSeal Sealing Heat Sealing Film, Ultra Clear, Peelable, qPCR, 100 films/bx, 5 bx/cs
PS1000-200AccuSeal Sealing Heat Sealing Film for Low Temp, Peelable, PCR, 100 films/bx, 5 bx/cs
PS1000-300AccuSeal Sealing Heat Sealing Film for Storage, Pierceable, Aluminum, 100 films/bx, 5 bx/cs
PS1000-400AccuSeal Sealing Heat Sealing Film for Compound Storage, Peelable, 100 films/bx, 5 bx/cs
PS1000-GP300AccuSeal Sealing Heat Sealing Film w/ Paper Back, General, PCR, 100 films/bx, 10 bx/cs
PS1000-GP111
AccuSeal Sealing Heat Sealing Film, Easy Pierce, 100 films/bx, 5 bx/cs
DISCONTINUED - Please 
contact us for more options.
 

 

Item#:
ASPCRFILM8

SmartSeal Semi-Automated Microplate Sealer

The Accuris SmartSeal™ Automated Plate Sealer provides fast, reproducible sealing of microplates. Unlike manual plate sealing, the SmartSeal ensures uniform sealing of all wells and consistency from one plate to the next.
  • Fast, reproducible sealing of well plates
  • Adjustable time, temperature and pressure for optimized sealing
  • User friendly controls, load plate and press run to seal
  • Accepts a wide range of plate types and heights, including deep well storage plates, assay plates, pcr plates, etc.
The Accuris SmartSeal™ Automated Plate Sealer provides fast, reproducible sealing of microplates. Unlike manual plate sealing, the SmartSeal ensures uniform sealing of all wells and consistency from one plate to the next.

With digital control over all parameters (time, temperature and pressure) the SmartSeal is capable of accepting a wide range of plate types and heights. An aluminum block adapter is included (for assay and PCR plates) increasing the compatibility of the instrument to allow for sealing nearly any plate up to 48mm tall.

With sealing times as low as 5 seconds, the SmartSeal is a time saving solution for labs and production facilities requiring high quality and consistent plate sealing.

Compatible with:

  • Deep well (storage) plates up to 48mm tall
  • Assay plates
  • PCR Plates (skirterd, semi-skirted and non-skirted)
  • Polypropylene polystyrene or Polyethylene plates
  • Foil or polymer (transparent) seals

Specifications:
 
  Model  Accuris SmartSeal
  Temp. Range  80°C to 200°C
  Temp. Accuracy  1°C
  Sealing Time  0.5 sec. to 10 sec. (0.1 sec. increments)
  Max. Plate Height  48 mm
  Dimensions  17.8 x 37 x 33 cm / 7 x 14.6 x 13 in.
  Weight  9.5 kg / 21 lbs
  Electrical 115V or 230V, 50-60Hz, 300W
Item#:
ASSMARTSEALTM
For PCR, qPCR, ELISA & General Storage


PureAmp Sealing Films and Foils

 

For PCR, qPCR, ELISA & General Storage.


PureAmp
Sealing Films are compatible with all standard PCR plates.

Film and foil options include standard adhesive pierceable aluminum, standard adhesive transparent, optically clear standard adhesive, optically clear non-tacky pressure activated adhesive, and low-adhesive EZ-peel, all of which are guaranteed to keep evaporation rates below 5%.

  • Multiple types for PCR, qPCR, and ELISA
  • All types available sterile or non-sterile
  • Evaporation rates < 5%
  • 10 inner bags of 10, 100 per pack
  • Certified RNase, DNase, DNA, & PCR inhibitor free

 

Item#:
ASPUREAMPSEALFILM

Adhesive Sealing Films

Soft and impermeable aluminum sealing foils with medical grade acrylic adhesive.  Once applied, the seal adheres aggressively, forming a tight positive seal preventing evaporation of VOCs, sample loss in storage, drying, cross contamination, and reducing the risk of aerosols and pathogens venting.

  • Suitable for sealing all standard PCR plates, micro plates, and deep well blocks.
  • Perfect for light sensitive samples
  • Easily pierced with single or multi-channel pipettors and probes
  • Withstands long term storage down to -80°C
  • Single tabbed backing paper removes easily; aluminum sheet resist rolling back.
Item#:
ASADHESISEALFIL

1.4 mL Aluminum Adhesive Sealing Film

Soft and non-permeable, the 1.4 mL aluminum sealing foil offers optimum sealing for all PCR plates. Using a medical-grade acrylic adhesive, our foil seals are easily pierced with single or multi-channel pipettors and robotic probes. These soft foil seals, resist rolling back when removing the backing paper and seal tightly to both PCR and Deep Well Blocks.

Ideally suited for PCR and Cold Storage down to -80 °C. Exceptional adhesive properties and foil material durability yield virtually no sample evaporation or drying.

Certified free of RNase, DNase, Pyrogen, and nucleic-acid contamination. 

Specifications 

Length133.35 mm
Length (w/ end tabs removed)120.65 mm
Width80.01 mm
Overall Thickness1.4 mil
Adhesive Thickness1.1 mil
MaterialAluminum
AdhesiveMedical Grade Acrylic
Max. Temperature120 °C
Min. Temperature-80 °C
Primary UsagesStorage or Sample Cover
Item#:
ASADHESEALFLALM14

SealPlate® RollMate Pre-scored Film

Convenient, Easy-to-Use Roll format.

Pre-scored film on a roll is a more economical and efficient alternative to rapidly seal microplates or racked test tubes; faster than applying single sheeted film. Self-standing, non-slip supports hold the pre-scored film roll securely in place for quick and easy bench-top dispensement. The roll unwind tension can be easily adjusted to user preference. Continuous backing liner can be torn off or fed directly into a waste basket for disposal.
  • Economical
  • Quicker, more efficient sealing
  • Provides a secure seal and is easy to remove
  • Rigid polyester film features good clarity and easy handling
  • Can be used as a temporary seal while awaiting further processing
Applications
  • General laboratory use
  • Short-term storage
  • Incubation
  • ELISA
Temperature Range
  • -40°C to +120°C

Proudly made in the USA! Click here for more made in America products!
 

EZcapFoilStrips

An economical and more user-friendly alternative to PCR strip caps; no sore thumbs and fingers guaranteed!

Application: PCR and light sensitive assays
Temp. Range: -20°C to +120°C

Precision cut to a smaller dimension for sealing one PCR 8-tube strip or a single row of a 96-well PCR or deep-well storage plate whenever rows must be
selectively protected or accessed. FoilStrips remove easily with no splash back as can occur with strip-caps.
  • Certified DNase- & RNase- free
  • Each sheet contains 6 strips on a continuous liner for ease of use
  • Pierceable with a pipet tip or robotic probe for direct sample recovery
  • The conformable nature of foil allows for the visual verification of the seal’s security around each well
  • 2.0 mil aluminum foil removes easily without tearing 
Helpful Tips:
  • A non-locking workstation is recommended for sample prep (e.g. 96-well round-boom plate)
  • Removal of the end-tabs at the perforations is recommended before cycling
  • A final sealing step is highly recommended in the thermal cycler because 8-tube strips flex during handling
  • Do not fold foil around sides of tubes

Proudly made in the USA! Click here for more made in America products!
 

AlumaSeal® CS

With a 50 uM layer of acrylic adhesive that withstands cold storage at temperatures to -80 °C.

Unlike other sealing films in the AlumaSeal® group, AlumaSeal® CS sealing films are not recommended for PCR or thermocycling. A single non-perforated end tab simplifies application. Simply hold the tab and strip the backing from the body of the sealing film as it lays on the plate to avoid curling. Dimensions are 82.6 by 132.6 mm, including the single 9.5 mm end tab. AlumaSeal® CS sealing films are certified DNase-, RNase-, and nucleic-acid-free.
  • Specially formulated for cold storage to -80 °C
  • Excellent barrier properties to retard evaporation
  • Easily pierceable with pipet tips or robotic probes for sample recovery 
*Completing application of AlumaSeal® CS sealing films by pressing with the accessory plate roller assures a secure uniform seal around all wells.

Proudly made in the USA! Click here for more made in America products!
 
 

FoilSeal RollMate Dispenser +1 Roll of FoilSeal Film

500 Pre-Scored Sheets.

Pre-scored film on a roll is a more economical and efficient alternative to rapidly seal microplates; faster than applying single sheeted film. Self-standing, non-slip supports hold the pre-scored film roll securely in place for quick and easy bench-top dispensement. The roll unwind tension can be easily adjusted to user preference. Continuous backing liner can be torn off or fed directly into a wastebasket for disposal.
  • Certified DNase- & RNase- free
  • Pierceable with a pipet tip or robotic probe for direct sample recovery
  • 2.0mil aluminum foil removes easily without tearing
  • The conformable nature of foil allows for the visual verification of the seal’s security around each well
  • Not recommended for long-term cold storage
Applications
  • PCR
  • Light sensitive assays
  • Short-term storage
  • Robotics
Temp. Range
  • -20°C to +120°C
Helpful Tips
  • End-tab removal before thermal cycling is highly recommended
  • Remove diagonally from a corner to prevent tearing

Proudly made in the USA! Click here for more made in America products!
 

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