PCR/qPCR

MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more.  If you are looking for any of these things, we can match almost all needs to all cyclers.  If you don’t find it easily contact us at tech@midsci.com and we will find it for you. 

96 or 384 we have it.  Non-Skirted, semi-skirted or full skirted, we have it.  0.1, 0.2, or 0.5 mL we have it.  A1, A12, H12, A24, P24 cuts, we have them.  Sybr© Green or probe alternative, we have it.  

Pro Tip: Colored plastics do not have any impact on DNA amplification.  But when setting up qPCR it is often recommended to use white plastics.  This will limit or prevent fluorescence refraction.  Light refraction can minimize sensitivity and consistency. 

Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips.  This will help optimize and reduce variance in the reactions.  Simple test, see the residual liquid left in tips?  This means some of the taq, dNTPs, sample, etc is left in the tip.  PR1MA tips help reduce that significantly and improve your consistency. 

Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate?  Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler.  Call us we have solutions.

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Amplification Plate
Please know that the manufacturer has suspended manufacturing this product indefinitely. 
We suggest purchasing PR1MA PCR Plastics.
Contact your rep or email tech@midsci.com for more information.
 

  • Standard thermal cycling and qPCR compatible
  • Ultra-thin and consistent wall thickness for precise thermal transfer
  • Single notch corner
  • Made from 99.9% virgin polypropylene
  • 340ul well capacity (working capacity dependent on closure method)
  • Fits standard thermal cyclers, plus ABI PCR 2700, 2720, 9700, 9800, 7300, 7500, 7900, and ABI sequencing units
  • Certified DNAse/RNAse and pyrogen-safe
Item#:
PCR-96M2-HS-C
Your Price:
42.96
Each
This item is discontinued

PRYME PCR plastics are designed and optimized for PCR & qPCR applications.

Unique design and development provides maximum mold and manufacturing accuracy. We outline precise product specifications and tolerances and minimize mold cavities while using CYCLERtest's temperature control competence in our manufacturing process. Result: superior and reproducible products with, in case of (q)PCR vessels, an average wall thickness of 0.30 mm for tubes and strips and 0.35 mm for plates, both with a maximum tolerance of 0.05 mm. Combined with the identical design of (q)PCR product closure one ends up in fully interchangeable and freely selectable (q)PCR cap and vessel combinations.

Extreme uniform wall thickness leads to a very even heating of the sample and more homogeneous reaction conditions, leading to more reproducible results. Extreme uniform wall thickness also leads to lower evaporation rates and therefore more consistent (q)PCR results. Evaporation rates of >10% up to 30% significantly influence the ratio of your (q)PCR components in the vessel, and thus the resulting reaction and its result. In case of optical detection via optical reading through the side of the qPCR tubes, better wall uniformity increases the signal consistency.

Clever design and sealing bands around the tube closure points provide a leak-free seal, reducing evaporation and leading to more reproducible results. These superior sealing properties allow for running (q)PCR reactions with volumes as low as 5 µl. The cap design allows minimum pressure for closure and opening which is highly appreciated by users and minimizes RSI risks.


Item#:
ASPRYMEERGPCRTUBE
Item#:
ASMIDSCIPCRCAP
Please know that the manufacturer has suspended manufacturing this product indefinitely. 
We suggest purchasing 
PR1MA PCR Plastics.
Contact your rep or email tech@midsci.com for more information.

 PCR Fast Plate, 96x0.1ml

  • Optimized for fast PCR and qPCR compatible
  • Ultra-thin and consistent wall thickness for precise thermal transfer
  • Made from 99.9% virgin polypropylene
  • 240ul well capacity (working capacity dependent on closure method)
  • Designed for ABI thermal cyclers 7500 and 7900, 9800, Veriti, and StepOne
  • Certified DNAse/RNAse and pyrogen-safe
Item#:
ASPRCFASTPLATE
Domed Cap, Clear, 1000/pk

Please know that the manufacturer has suspended manufacturing this product indefinitely. 
We suggest purchasing PR1MA PCR Plastics.
Contact your rep or email tech@midsci.com for more information.


 
  • Ultra-thin and consistent wall thickness for precise thermal transfer
  • Full-length insertion cap design prevents sample evaporation
  • Made of 99.9% pure virgin polypropylene
  • Certified DNAse/RNAse, and pyrogen-safe
Item#:
PCR-02D-C
Your Price:
629.65
Each

PR1MA PCR Tool
 
Tool for opening and/or closing PCR strips or single tubes.  Compatible with flat or domed caps.  Can also be used for sealing film.

Item#:
ASPCRTOOL
Half-Skirt, Low-Profile, Clear
Please know that the manufacturer has suspended manufacturing this product indefinitely. 
We suggest purchasing 
PR1MA PCR Plastics.
Contact your rep or email tech@midsci.com for more information.

 
  • Optimized for fast PCR and qPCR compatible
  • Ultra-thin and consistent wall thickness for precise thermal transfer
  • Made from 99.9% virgin polypropylene
  • 240ul well capacity (working capacity dependent on closure method)
  • Designed for ABI thermal cyclers 7500 and 7900, 9800, Veriti, and StepOne
  • Certified DNAse/RNAse and pyrogen-safe
Item#:
PCR-96-LP-AB-C
Your Price:
154.77
Each
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