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PCR/qPCR

MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more.  If you are looking for any of these things, we can match almost all needs to all cyclers.  If you don’t find it easily contact us at tech@midsci.com and we will find it for you. 

96 or 384 we have it.  Non-Skirted, semi-skirted or full skirted, we have it.  0.1, 0.2, or 0.5 mL we have it.  A1, A12, H12, A24, P24 cuts, we have them.  Sybr© Green or probe alternative, we have it.  

Pro Tip: Colored plastics do not have any impact on DNA amplification.  But when setting up qPCR it is often recommended to use white plastics.  This will limit or prevent fluorescence refraction.  Light refraction can minimize sensitivity and consistency. 

Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips.  This will help optimize and reduce variance in the reactions.  Simple test, see the residual liquid left in tips?  This means some of the taq, dNTPs, sample, etc is left in the tip.  PR1MA tips help reduce that significantly and improve your consistency. 

Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate?  Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler.  Call us we have solutions.

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  • The CleanSpot is designed to fulfill a very important laboratory need...control of PCR contamination.
  • Just 25 - 30 minutes of UV irradiation in the CleanSpot can prevent unwanted PCR contamination.
  • The combination of a special light reflector and two powerful overhead UV bulbs generate Pyrimidine Dimers (T,C) and other photo defects in contaminating target sequences, thus eliminating falsely primed products that result in countless lost time and expense.
  • The CleanSpot is designed to prevent unwanted contaminant DNA in amplification mixtures, thereby saving many lost hours and expensive samples.
  • The UV resistant acrylic enclosure provides a contained, dead air space in which to set up your reactions and permits safe 254nm UV irradiation of solutions and supplies. This powerful UV wavelength renders DNA contaminants biologically inactive in 30 minutes or less.
  • The thick front panel is an effective beta irradiation shield as well as acting as a breath guard. An overhead fluorescent light illuminates the work area.
  • Dimensions: 18"L x 24"W x 28"H
  • 1 year warranty
Item#:
CS01
Your Price:
2984.00
Each
This item is discontinued
with 500 Color Coding Inserts

The Rotocycler 72 facilitates the handling and insertion of caps on the tubes by offering a special solid aluminum loading rack. This rack can hold up to 18 x 0.1 mL tube strips for a total of 72 tubes. Other cavities can hold larger reaction tubes. To keep reactions cool during setup, simply place the rack in a refrigerated area. For easy reference, all wells are numbered. Color coding is made possible by inserting a Capinsert in up to two locations on the rack.


Includes: Rotocycler 72 and 500 assorted color coding inserts


Accessories for the Rotocycler 72


T319-4N

Rotocycler 0.1 mL Tube and Cap Strips for Qiagen Rotor-Gene Q 
Real-Time Rotary Analyzer

T319-72D2

Rotocycler 72 Rotor with 100 µL wells for Qiagen Rotor-Gene Q 
Real-Time Rotary Analyzer

T319-100D1

Rotocycler 100 Rotor with 25 µL wells for Qiagen Rotor-Gene Q 
Real-Time Rotary Analyzer
Item#:
T319-4WS1
Your Price:
720.00
Each
Format, 24/cs, 100uL tubes for

The RotoCycler Discs are specially made to be used with the Qiagen Rotor-Gene Q Real-Time Rotary Analyzer. Two models are available: a 72-well format with 100 µL tubes and a 100-well format for reactions up to 25 µL. The discs a"one-piece "plate" equivalent, having vertically oriented wells compatible with automated reaction setup using a robotic liquid handling system.

Item#:
T319-72D2
Your Price:
303.00
Each

Gel Cutting Tips

  • Safely excise bands from gels
  • Avoid cross contamination
  • One-handed operation for quick and accurate gel cutting
  • Safer than using razor blades and won't scratch transilluminators
  • 1.1 x 4 mm
Cut gel with pipet tip on the end of a standard 1000µl pipettor. Gel piece is suspended in tip when pipettor is lifted from the gel. Expel the gel piece by pushing the pushbutton on the pipettor. Tip is ejected in normally using the ejector button.
 
Item#:
ASGELTIP1_1X4
10 rxns
5X RNA Gel Loading Kit
  • Reagents for denaturing and loading RNA samples onto a formaldehyde gel, using MOPS as a buffer
  • RNA sample is dissolved in 10µl of DEPC water and mixed with 35µl of denaturing solution. Heat the sample to 65°C for 5 min. Once the solution has cooled, add 5µl of loading dye. The sample is now ready to load into the gel.
  • DNase/RNase/Protease free
Item#:
IB01015
Your Price:
63.18
Each
Format, 30/cs, 25uL tubes for

The RotoCycler Discs are specially made to be used with the Qiagen Rotor-Gene Q Real-Time Rotary Analyzer. Two models are available: a 72-well format with 100 µL tubes and a 100-well format for reactions up to 25 µL. The discs a"one-piece "plate" equivalent, having vertically oriented wells compatible with automated reaction setup using a robotic liquid handling system.

Item#:
T319-100D1
Your Price:
392.00
Each
5ml
6X Loading Dye
    • Used for agarose electrophoresis of DNA, RNA or nucleic acids
    • Contains 3 tracking dyes and 15% Ficoll in a special Tris dye
      • Light blue - around 4000bp in 1% agarose
      • Indigo - around 600bp in 1% agarose
      • Magenta - around 150bp in 1% agarose
    • DNase/RNase/Protease free
Item#:
IB01010
Your Price:
72.26
Each
Discontinued, Contact us for more options!

This brand is being discontinued, please contact us for other options.

Need great dNTPs at a great price? Try our PR1MA™ dNTPs 

 

Bullseye dNTP Mix - 12.5 mM

  • Mix of dATP, dCTP, dGTP, dTTP
  • Each nucleotide is at a concentration of 12.5 mM
  • Ready-to-use molecular grade dNTP solution for use in DNA polymerization, DNA labelling and sequencing processes
  • High purity: >98% by HPLC
  • Supplied in solution at pH 7.5
  • dNTPs are stable at -20°C, avoid multiple freeze/thawing (For long-term usage, aliquoting is recommended)
  • Functionally tested with thermostable polymerases
Item#:
ASPCRDNTP1
Discontinued, Contact us for more options!


This brand is being discontinued, please contact us for other options.
 

Need great dNTPs at a great price? Try our PR1MA™ dNTPs 
 


Bullseye dNTP Mix - 10 mM 

  • Mix of dATP, dCTP, dGTP, dTTP
  • Each nucleotide is at a concentration of 10 mM
  • Ready-to-use molecular grade dNTP solution for use in DNA polymerization, DNA labelling and sequencing processes
  • High purity: >98% by HPLC
  • Supplied in solution at pH 7.5
  • dNTPs are stable at -20°C, avoid multiple freeze/thawing (For long-term usage, aliquoting is recommended)
  • Functionally tested with thermostable polymerases
Item#:
ASPCRDNTP2
Includes Writer and

Qamp Mini Portable PCR Thermocycler
  • Compact in Size: 102 x 136 x 104 mm
  • Preprogramed Chip: with unlimited applications
  • Touch Start: One Button to Go!
  • LCD Display: for customers to define status easily!
  • Portable: Only 1 kg

 

Qamp Mini is designed to achieve polymerase chain reaction (PCR) for the amplification of the targeted DNA in a miniature device making molecular biology more fun in the field. The Qamp Mini contains a centrally positioned Peltier heating and cooling module for 1-8 samples.  The design leads to accuracy in analysis and cost efficiency without sacrificing performance and quality.  With the compact size and One-Click to Go design Qamp Mini is the ideal instrument for laboratories or classrooms and in the fields of epidemiology, veterinary, food testing, pathogen detection, ecology, archaeology research and others.



Sample Number

8 (2 x 4 wells)

Temperature Range

4°C - 99°C

Max Heating Rate (°C/sec.)

4.6°C

Max Cooling Rate (°C/sec.)

3.4°C

Temperature Accuracy

± 0.4°C

Temperature Uniformity Across Block

± 0.4°C

Max No. of Cycles

unlimited

Max No. of Steps

unlimited

Display

LCD

Heated Lid

105°C Fixed (pre-heat to 60°C)

Dimensions (L x W x H)

10 x 13 x 10 cm

Weight

1 Kg

Power

VAC 100-240, 50/60 Hz, 120 W
Item#:
C310200
Your Price:
1687.50
Each
Item is no longer available.


Please know that the manufacturer has suspended manufacturing this product indefinitely. 
Contact your trusted MIDSCI Lab Consultant
or email tech@midsci.com for more information.

 


PRIME G (gradient) Thermal Cycler

  • Colour touchscreen for fast program setup
  • Small space-saving footprint
  • Temp Range 4°C to 100°C
  • Fast ramp rate of up to 3.0°C/sec
  • Larger block sizes; 48 x 0.2ml, 30 x 0.5ml and half a 96 well plate in horizontal format.
  • Gradient can be applied across a Temp Range of 30°C to 80°C
  • Maximum Gradient 30°C
  • Gradient calculator function
  • Data transfer via USB
  • 4 year warranty

The 3 PRIME G is a small gradient thermal cycler that builds on all the features of the 3 PRIME X instrument. The 48 well block format offers eight columns for annealing temperature optimisation and six rows for optimising reagents such as MgCl2 and primer concentrations. Annealing temperatures can be optimised over 30oC between temperatures 30°C to 80°C. The gradient calculator function displays the temperature for each of the eight columns, ensuring easy replication of thermal conditions.


Technical Specification


Sample capacity: 0.2ml

48

Sample capacity: 0.5ml

30

Maximum heating rate

3.0°C/s

Block Temp Range

4 °C to 100°C

Block uniformity at 50°C

<±0.3°C

Temperature accuracy at 50°C

<±0.2°C

Gradient Range

30°C to 80°C

Maximum Gradient

30°C

Minimum Gradient

1°C

Type

4 peltier element/block

Maximum cooling rate

2.1°C/s

Selectable heated lid temperature

100 °C to 115°C or off

Heated Lid pressure

Adjustable, dependent on consumables

Program interface

3.5" VGA colour touchscreen with graphical display

Maximum number of programs stored

1000

Data transfer

USB port

Auto re-start on power failure

Yes

Dimensions (L x W x H)

350 x 210 x 180mm

Weight

6kg

Voltage

100-230V, 50-60Hz

Power

155W
Item#:
ASPCRTC1


Please know that the manufacturer has suspended manufacturing this product indefinitely. 

Contact your trusted MIDSCI Lab Consultant
or email tech@midsci.com for more information.




Key benefits
Speed

  • 40 cycles in 40 minutes before fast optimization.
  • 40 cycles in 15 minutes when optimized.
  • Genotype in 4 minutes with over 99% accuracy.
  • Set up a 4plex reaction in only 4 mouse clicks.

    Confidence
  • MIQE compliant - always consistent, always compliant.
  • Instant settle time to achieve world leading uniformity of ±0.1°C across the block - any well, any instrument - consistent results in any system.
  • 400 specific qPCR reagent kits available.


    Performance
  • Consistent results provide trust and confidence. Well-to-well, run-to-run , machine-to-machine.
  • Compile data from multiple experiments. Pool plates and results across labs and instruments.
  • Record every well, with every filter, in every cycle. Never miss a data point.
  • Adaptive LED Control means you never have uncertainty of saturated unknowns, no bleaching though light to neighboring wells or maximizing the wide dynamic range. Trust results are correct. 
  • All other block-based qPCR machines have lower uniformity. If you perform Next Generation Sequencing, any other instrument than Prime Pro 48 is risking your NGS, with significant cost implications.

    Value
  • No other qPCR system offers so much performance for such a low price.
  • Available as a complete system - Techne instrument, plates, seals and reagents.
  • Alternatively, is chemically open platform - use your preferred supplier reagents.
  • Lowest price per run - use duplicates and low sample volumes. Don't waste money with triplicates and large sample volumes.
  • Everything as standard - no add-ons, HRM as standard, unlimited software license. 
  • Highly accurate and uniform qPCR prevents means successful NGS runs and significant moneysaving.

    Sensitivity
  • ±0.1°C uniformity across the whole block instantly after every temperature change means that any well, in any instrument, provides same answer
  • HRM - Identify Class IV SNP >99.9% of the time
  • Unsurpassed block uniformity with patented block technology enables the researcher to use duplicates on Prime Pro 48, whereas triplicates are required on less uniform competitor machines.

     

    The Prime Pro 48 real time PCR system is a new, high specification, economically priced real time thermal cycler from Techne. Made in the UK, the Prime Pro 48 features the highest thermal block uniformity and fastest run time of any block-based qPCR system on the market.

    High thermal uniformity and validated sample volumes down to 5ul ensures Prime Pro 48 allows researchers to bring run times for an optimized 40 cycle reaction down to only 15 minutes. PCR efficiency relies on precise temperature control during the denaturation and annealing steps. For the highest accuracy, temperature must remain uniform across the plate, ensuring that all samples are processed equally. The unique thermal block design of Prime Pro 48 achieves this with a
    unique heating and cooling system that quickly cycles from one temperature to the next and then achieves a uniform ±0.1°C in every well of the block within a fraction of a second of reaching each dwell temperature. No other block-based qPCR system can achieve similar performance.

    The capacity of Prime Pro 48 accommodates a unique and economical 48-well PCR plate, the size of which is only 1/8 the size of a standard 96-well plate. The well format mirrors a 384-well plate therefore allowing the use of a 16-channel micropipette. The small plate cuts reagent costs in half whilst still producing a strong fluorescence signal. Minimizing the plate size also significantly improves thermal uniformity.

    Technical Specification

    High Resolution Melt

    Yes

    Volume per well

    Validated for 5 to 20 ul

    Detection sensitivity

    1 copy

    Temp uniformity

    +- 0.1 °C within 1 sec

    Temp stability

    +- 0.1 °C

    Temperature range

    30 to 100 °C

    Average ramp rate

    5.5 °C sec

    Thermal system

    Hollow silver block peltier-based with conductive fluid

    Block format

    48-well block

    Consumables

    48-well custom plates and optical adhesive seals

    Optical system

    Dual LED excitation (452 to 486 nm and 542 to 582 nm)

    Camera

    CCD camera

    Filters

    4 emission filters (505 to 545nm 562 to 596nm 604 to 644nm 665 to 705nm)

    Calibrated dyes

    SYBR FAM HEX ROX Cy5

    Additional dyes

    All dyes within range compatible with no additional calibration

    Passive reference dyes

    Use of ROX is supported but optional

    Data collection

    Data always collected in all four filters for all wells

    Plate setup

    Plate setup for data analysis can be altered after run completes

    Melt curve

    Supports continuous data acquisition in a single filter to provide increased data point collection and reduced run times

    PCR cycle time

    40 cycles in less than 40 minutes

    PCR cycle time (FAST optimized)

    40 cycles in less than 20 minutes

    Dynamic range

    Greater than 9 logs

    Calibration

    Not required

    Installation

    Plug and play design

    Precision

    Discriminates 5000 and 10000 template copies with 99 percent confidence

    Warranty

    1 year (parts and labor included)

    Voltage

    120 to 240 V

    Frequency

    50 and 60 Hz

    Nominal current draw

    8A

    Peak power

    500W (typical power is 180W)

    Software

    Multiple license software included at no additional cost

    Chemistry

    All chemistries supported

    Applications

    Absolute Quantification and Relative Quantification

    Applications

    Allelic Discrimination and High Resolution Melt

    Dimensions closed (WxDxH)

    34.5cm x 31cm x 32cm (13.6 in x 12.2 in x 12.6 inches)

    Dimensions open (WxDxH)

    34.5cm x 31cm x 37cm (13.6 in x 12.2 in x 14.5 inches)

    Weight

    13.6 Kg (30 lb)

     

     

    Ordering information

    Part number

    Description

    PRIMEPRO48

    Prime Pro 48 Real Time PCR System, includes Accessories and Software (Pro and ProControl). PC not supplied

    PROPLATE48

    Pack of 50 Real Time PCR Plates, 48-wells

    PROSEAL48

    Pack of 50 Real Time PCR Plate seals
Item#:
ASPCRTC7
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