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PCR/qPCR
MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more. If you are looking for any of these things, we can match almost all needs to all cyclers. If you don’t find it easily contact us at tech@midsci.com and we will find it for you.
96 or 384 we have it. Non-Skirted, semi-skirted or full skirted, we have it. 0.1, 0.2, or 0.5 mL we have it. A1, A12, H12, A24, P24 cuts, we have them. Sybr© Green or probe alternative, we have it.
Pro Tip: Colored plastics do not have any impact on DNA amplification. But when setting up qPCR it is often recommended to use white plastics. This will limit or prevent fluorescence refraction. Light refraction can minimize sensitivity and consistency.
Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips. This will help optimize and reduce variance in the reactions. Simple test, see the residual liquid left in tips? This means some of the taq, dNTPs, sample, etc is left in the tip. PR1MA tips help reduce that significantly and improve your consistency.
Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate? Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler. Call us we have solutions.
PR1MA™ T4 Gene 32 Protein
PR1MA™ T4 Gene 32 Protein (T4 gp32) is a single-stranded DNA binding (ssDNA) protein required for E. coli bacteriophage T4 replication. It binds and stabilizes ssDNA structures which facilitates electron microscopic examination, and has also been shown to improve restriction digests, improve T4 DNA polymerase activity, and increase the yield of PCR reactions, including those with long amplicons.
- Optimal temperature: 37°C
- Heat inactivation: 65°C for 20 minutes
- Storage temperature: -20°C
- 10X T4 gp32 reaction buffer included
Buffer composition
- 50% glycerol
- 50 mM Tris-HCl
- 50 mM KCl
- 1 mM DTT
- 0.1 mM EDTA
- 0.1% Tween-20
- pH = 7.5
*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.
Proudly made in the USA! Click here for more made in America products!
PR1MA™ Reverse Transcriptase
PR1MA™ reverse transcriptase (RT) is an RNA-dependent DNA polymerase that can be used for complementary DNA (cDNA) synthesis from an RNA template and is ideal for use in molecular amplification assays. PR1MA™ RT is a robust enzyme that works in a broad range of temperatures (40 - 72°C) and has RNase H activity.
- Optimal temperature: 55°C
- Heat inactivation: 75°C for 20 minutes
- Glycerol-free buffer available
- Storage temperature: -20°C (standard buffer)
- 10X Isothermal buffer included
Standard Buffer Composition
- 50% glycerol
- 10 mM Tris-HCl
- 100 mM KCl
- 1 mM DTT
- 0.1 mM EDTA
- pH = 7.5
Important note: Please be sure to use the buffer provided with this product to ensure optimal results.
*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.
Proudly made in the USA! Click here for more made in America products!
PR1MA™ Reverse Transcriptase, RNase H -
PR1MA™ reverse transcriptase (RT) is an RNA-dependent DNA polymerase ideal for use in RT-PCR and first-strand synthesis of complementary DNA (cDNA) for generation of cDNA libraries from single-stranded RNA, DNA, or RNA:DNA hybrids.
- Decreased RNase H activity enables longer cDNA synthesis (>5 kb).
- Lacks 3’ to 5’ exonuclease activity
- Optimal temperature at 42°C
- Temperature range: 40 to 50°C
- Heat inactivation: 70°C for 20 minutes
- Storage temperature: -20°C
- 10X reaction buffer included
Buffer composition
- 50% glycerol
- 50 mM Tris-HCl
- 50 mM KCl
- 1 mM DTT
- 0.1 mM EDTA
- pH = 7.5
*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.
Proudly made in the USA! Click here for more made in America products!
2025 LabValues Promotion!For a limited time enjoy discounted pricing on theAccuSeal™ Semi-Automated Plate Sealer (PS1000)!
Special price applies only to the specified item; no substitutions.
Cannot be combined with other discounts or pricing. Not valid on prior purchases.
Promo is valid 01/06/2025 – 06/30/2025 or while supplies last.
All terms and conditions apply.
AccuSeal™ Semi-Automated Plate Sealer
AccuSeal™ Semi Automated Plate Sealers from MIDSCI are engineered and constructed by Labnet International, Inc to maximize PCR, assay, and storage applications with a user-friendly panel and automated sealing options.
Great emphasis has been placed on incorporating a wide range of standard heights to deep well microplates with appropriate adaptors to efficiently fit the plates for consistent temperature regulation. The AccuSeal™ sealer is optimized for simple digital control and operation, user-friendly versatility and convenience as well as safety.
Controls and Operation
- Intuitive layout permits optimal sealing time, temperature and settings
- Includes a "No Heat" setting for pressure sealing applications
Versatility and Convenience
- Adaptors specific for a PCR plate or an assay plate accommodate a variety of fits
- Automatically adjusts heat for different height plates
- Provides uniform pressure for a quality seal with a special non-stick coating to prevent messy cleanup.
Safety
- Motorized drawer features safety switches and prevents injury to the user.
Specifications
Dimensions | 6.75 x 12.75 x 14.25 in. |
Weight | 24 Lbs |
Sealing Temperature | OFF, 100 to 190 °C |
Temperature Accuracy | +/- 1.00 °C |
Temperature Uniformity | +/- 1.00 °C |
Sealing Time | 0.5 to 10 sec |
Compatible Plate Materials | PP (Polypropylene) PS (Polystyrene) PE (Polyethylene) |
Compatible Plate Types | Standard Assay Plates Deep-well Storage Plates PCR Plates: skirted, semi-skirted, non-skirted |
Maximum Plate Height | 45 mm |
Compatible Sealing Film Types | Foil-polypropylene laminate Clear polyester-polypropylene laminate Clear polymer Thin clear polymer Foil-laminate Foil |
Adapters
PS1000-PCR | AccuSeal™ PCR plate adapter for sealing PCR and 384 well plates |
PS1000-ADAPT | AccuSeal™ Assay plate adapter for sealing assay plates |
Sealing Film
PS1000-150 | AccuSeal™ Sealing Heat Sealing Film, Ultra Clear, Peelable, qPCR, 100 films/bx, 5 bx/cs |
PS1000-200 | AccuSeal™ Sealing Heat Sealing Film for Low Temp, Peelable, PCR, 100 films/bx, 5 bx/cs |
PS1000-300 | AccuSeal™ Sealing Heat Sealing Film for Storage, Pierceable, Aluminum, 100 films/bx, 5 bx/cs |
PS1000-400 | AccuSeal™ Sealing Heat Sealing Film for Compound Storage, Peelable, 100 films/bx, 5 bx/cs |
PS1000-GP300 | AccuSeal™ Sealing Heat Sealing Film w/ Paper Back, General, PCR, 100 films/bx, 10 bx/cs |
PS1000-GP111 | AccuSeal™ Sealing Heat Sealing Film, Easy Pierce, 100 films/bx, 5 bx/cs DISCONTINUED - Please contact us for more options. |
SmartSeal™ Semi-Automated Microplate Sealer
- Fast, reproducible sealing of well plates
- Adjustable time, temperature and pressure for optimized sealing
- User friendly controls, load plate and press run to seal
- Accepts a wide range of plate types and heights, including deep well storage plates, assay plates, pcr plates, etc.
- Deep well (storage) plates up to 48mm tall
- Assay plates
- PCR Plates (skirterd, semi-skirted and non-skirted)
- Polypropylene polystyrene or Polyethylene plates
- Foil or polymer (transparent) seals
Model | Accuris SmartSeal |
Temp. Range | 80°C to 200°C |
Temp. Accuracy | 1°C |
Sealing Time | 0.5 sec. to 10 sec. (0.1 sec. increments) |
Max. Plate Height | 48 mm |
Dimensions | 17.8 x 37 x 33 cm / 7 x 14.6 x 13 in. |
Weight | 9.5 kg / 21 lbs |
Electrical | 115V or 230V, 50-60Hz, 300W |
PureAmp™ Sealing Films and Foils
For PCR, qPCR, ELISA & General Storage.
PureAmp™ Sealing Films are compatible with all standard PCR plates.
Film and foil options include standard adhesive pierceable aluminum, standard adhesive transparent, optically clear standard adhesive, optically clear non-tacky pressure activated adhesive, and low-adhesive EZ-peel, all of which are guaranteed to keep evaporation rates below 5%.
- Multiple types for PCR, qPCR, and ELISA
- All types available sterile or non-sterile
- Evaporation rates < 5%
- 10 inner bags of 10, 100 per pack
- Certified RNase, DNase, DNA, & PCR inhibitor free
Adhesive Sealing Films
Soft and impermeable aluminum sealing foils with medical grade acrylic adhesive. Once applied, the seal adheres aggressively, forming a tight positive seal preventing evaporation of VOCs, sample loss in storage, drying, cross contamination, and reducing the risk of aerosols and pathogens venting.
- Suitable for sealing all standard PCR plates, micro plates, and deep well blocks.
- Perfect for light sensitive samples
- Easily pierced with single or multi-channel pipettors and probes
- Withstands long term storage down to -80°C
- Single tabbed backing paper removes easily; aluminum sheet resist rolling back.
1.4 mL Aluminum Adhesive Sealing Film
Specifications
Length | 133.35 mm |
Length (w/ end tabs removed) | 120.65 mm |
Width | 80.01 mm |
Overall Thickness | 1.4 mil |
Adhesive Thickness | 1.1 mil |
Material | Aluminum |
Adhesive | Medical Grade Acrylic |
Max. Temperature | 120 °C |
Min. Temperature | -80 °C |
Primary Usages | Storage or Sample Cover |
SealPlate® RollMate™ Pre-scored Film
Convenient, Easy-to-Use Roll format.Pre-scored film on a roll is a more economical and efficient alternative to rapidly seal microplates or racked test tubes; faster than applying single sheeted film. Self-standing, non-slip supports hold the pre-scored film roll securely in place for quick and easy bench-top dispensement. The roll unwind tension can be easily adjusted to user preference. Continuous backing liner can be torn off or fed directly into a waste basket for disposal.
- Economical
- Quicker, more efficient sealing
- Provides a secure seal and is easy to remove
- Rigid polyester film features good clarity and easy handling
- Can be used as a temporary seal while awaiting further processing
- General laboratory use
- Short-term storage
- Incubation
- ELISA
- -40°C to +120°C
Proudly made in the USA! Click here for more made in America products!
EZcap™FoilStrips
An economical and more user-friendly alternative to PCR strip caps; no sore thumbs and fingers guaranteed!- Certified DNase- & RNase- free
- Each sheet contains 6 strips on a continuous liner for ease of use
- Pierceable with a pipet tip or robotic probe for direct sample recovery
- The conformable nature of foil allows for the visual verification of the seal’s security around each well
- 2.0 mil aluminum foil removes easily without tearing
- A non-locking workstation is recommended for sample prep (e.g. 96-well round-boom plate)
- Removal of the end-tabs at the perforations is recommended before cycling
- A final sealing step is highly recommended in the thermal cycler because 8-tube strips flex during handling
- Do not fold foil around sides of tubes
Proudly made in the USA! Click here for more made in America products!
AlumaSeal® CS™
- Specially formulated for cold storage to -80 °C
- Excellent barrier properties to retard evaporation
- Easily pierceable with pipet tips or robotic probes for sample recovery
Proudly made in the USA! Click here for more made in America products!
FoilSeal™ RollMate™ Dispenser +1 Roll of FoilSeal™ Film
500 Pre-Scored Sheets.
- Certified DNase- & RNase- free
- Pierceable with a pipet tip or robotic probe for direct sample recovery
- 2.0mil aluminum foil removes easily without tearing
- The conformable nature of foil allows for the visual verification of the seal’s security around each well
- Not recommended for long-term cold storage
- PCR
- Light sensitive assays
- Short-term storage
- Robotics
- -20°C to +120°C
- End-tab removal before thermal cycling is highly recommended
- Remove diagonally from a corner to prevent tearing