Bullseye Individual dNTP's

• Ready-to-use molecular grade dNTP solution for use in DNA polymerization, DNA labeling and sequencing processes
• High purity: >98% by HPLC
• Supplied in solution at pH 7.5
• dNTPs are stable at -20°C, avoid multiple freeze/thawing (For long-term usage, aliquoting is recommended)
• Functionally tested with thermostable polymerases

• Mix of dATP, dCTP, dGTP, dTTP
• Each nucleotide is at a concentration of 100 mM
• Ready-to-use molecular grade dNTP solution for use in DNA polymerization, DNA labelling and sequencing processes
• High purity: > 98% by HPLC
• Supplied in solution at pH 7.5
• dNTPs are stable at - 20°C, avoid multiple freeze/thawing (For long-term usage, aliquoting is recommended)
• Functionally tested with thermostable polymerases 

dNTP SET

(dATP, dCTP, dGTP, dTTP)

100 mM

This brand is being discontinued and will only be available while supplies last.
 

Need a great DNA Safe Stain at a great price?
Try PR1MA! Click here to order.
 

Bullseye DNA Safe Stain Plus

• Higher sensitivity
• Use in the same way as EtBr in agarose gel electrophoresis
• Detection of DNA and RNA
• Safest DNA stain by far
• Low cost
• 10,000X
• Excitation wavelengths at 290nm and 490nm

DNA SafeStain Plus is a highly sensitive green fluorescent DNA/RNA staining reagent for detecting nucleic acids in agarose and polyacrylamide gels. This unique stain gives high sensitivity for detection of double-stranded or single-stranded DNA and RNA. Gels can be post-stained or the stain can be added to gels during gel casting or to the gel running buffer. DNA SafeStain Plus has two excitation wavelength peaks at about 290nm and 490nm, and an emission wavelength at 530nm, making it compatible with a standard UV light box, a blue-light transilluminator, or a gel reader equipped with visible light excitation; such as, a 488 nm laser-based gel scanner.

DNA SafeStain Plus is in a 10,000X concentrated format that can be easily diluted 10,000 times for use in precast gel staining, or 5,000 times for use in post gel staining.

StripSpin™ 12 Mini Centrifuge has been discontinued. A replacement model (C2248) can be found here.

StripSpin™ 12 Mini Centrifuge

• Unique rotor for 12-strip PCR tubes
• Also can be used with 8-strip and individual PCR tubes
• Starts and Stops with opening/closing the lid
• Tiny benchtop footprint and affordability
• Place a StripSpin at the bench, thermal cycler & water bath for convenience.

Specifications:

Bullseye RT 2X Master Mix

• Up to 9kb cDNA synthesis
• Ensures sample to sample consistency
• Large RNA sample volume capacity
• Ready to use

Size: 100 rxns

RT 2X Master Mix is a proprietary, ready-to-use master mix for first-strand cDNA synthesis in a 2X concentration. This optimized reaction mix contains ribonuclease inhibitor, dNTPs, and a balanced concentration for oligo(dT) and random primers. The ribonuclease inhibitor effectively protects RNA template from degradation. The oligo(dT) anneals selectively to the poly(A) tail of mRNAs and the random primers do not require the presence of poly(A) and they are utilized for the transcription of mRNA 5-end regions. The resultant cDNA can be directly used as template in different PCR experiments.

Kit Components

Storage

• Store at -20°C in a frost-free freezer.

Application

• cDNA synthesis
• Construction of cDNA libraries
• Generation of probes for hybridization 

Protocol

1. Thaw RNA templates and all reagents on ice. Mix each solution by vortexing.
2. Assemble the following components in a tube on ice, and mix well:

1. Heat the mixture at 65°C for 5 mins and incubate on ice for at least 1 min.
2. Collect all components by a brief centrifugation and add 1 µl of the EasyScript RTase to the tube.
3. Mix well and collect all the components by a brief centrifugation.
4. Incubate the tube at room temperature for 10 min for annealing.
5. Perform cDNA synthesis by incubating the tube for 50 min at 42°C.
6. Stop the reaction by heating it at 85°C for 5 min.
7. Chill on ice. The newly synthesized first-strand cDNA is ready for immediate downstream applications. 

Bullseye EasyScript cDNA Synthesis Kit

Application

• First strand cDNA synthesis for PCR
• Construction of cDNA libraries
• Generation of probes for hybridization

EasyScript cDNA Synthesis Kit is a complete system for the efficient synthesis of first strand cDNA from RNA templates. The recombinant RNasin Ribonuclease Inhibitor, supplied with the kit effectively protects RNA template from degradation.

The kit is also supplied with both oligo(dT) and random primers. The oligo(dT) anneals selectively on the poly(A) tail of mRNA. Random primers do not require the presence of poly(A). Therefore, they can be used for transcription of the 5'-end regions of mRNA. Gene-specific primers may also be used with the kit. The first strand of cDNA can be directly used as a template in PCR.

EasyScript Reverse Transcriptase (RTase) within the kit is a genetically modified form of Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV). Both the EasyScript and EasyScript Plus are RNase H deficient (negative). The enzyme is purified from bacteria host as a single holoenzyme of 71 kDa with the capacity of first-strand cDNA synthesis of template up to 9kb and can be used for routine cDNA synthesis.

Kit Components

Storage Buffer
 
50 mM Tris-HCl (pH 8.3), 100 mM NaCl, 0.1 mM EDTA, 5 mM DTT, 0.1% (v/v) Triton X-100, and 50% (v/v) glycerol.

Storage
 
Store at -20°C in a frost-free freezer. Multiple freezing and thawing of RNA should be avoided. Keep RNA on ice all the time. It is recommended that the first strand cDNA synthesis is carried out under conditions where RNase contamination has been eliminated.

General Protocol

RT-PCR reactions should be assembled in a RNA-free environment. The use of "clean", automatic pipettes designated for PCR and aerosol resistant barrier tips are recommended. 
1. Thaw template RNA and all reagents on ice. Mix each solution by vortexing, and centrifuge briefly to collect residual liquid from the sides of the tubes.
2. Prepare the following reaction mixture in a PCR tube on ice:

3. Incubate at 25°C for 10 minutes if random primer is used. Omit this step if Oligo(dT) primer or sequence specific primer are used.
4. Incubate the mixture at 42°C for 60 minutes.
5. Stop the reaction by heating at 85°C for 5 minutes.
6. Chill on ice. The newly synthesized first-strand cDNA now can be used directly for PCR amplification.

Notes
 
1. Isolation of poly(A)+ RNA from total RNA is not mandatory; however, doing so may improve the yield and purity of the final product.
2. RNA sample must be free of contaminating genomic DNA.
3. Unlike the oligo(dT) priming, which usually requires no optimization, the ratio of a random primer to RNA is critical in terms of the average length of cDNA synthesized in the reaction. Increasing the ratio of random primer/RNA will result in higher yield of shorter (~500 bp) cDNA, whereas decreasing this ratio will produce longer products.

4. The synthesized cDNA should be stored at -20°C.

Bullseye EasyScript™ Reverse Transcriptase

Application

• Synthesis cDNA froma single-stranded RNA or DNA primer extension
• Sequencing dsDNA
• cDNA library
• Template production for use in PCR
• 3'-end labeling of duplex DNA via end-filling reactions

EasyScript™ Reverse Transcriptase is a genetically modified form of Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV). Both the EasyScript™ and EasyScript™ Plus are RNase H deficient (negative). The enzyme is purified from bacteria h o s t as a single holoenzyme of 71 kDa with the capacity of first-strand cDNA synthesis of template up to 9kb and can be used for routine cDNA synthesis.

Kit Components

Storage Buffer
 
50 mM Tris-HCl (pH 8.3), 100 mM NaCl, 0.1 mM EDTA, 5 mM DTT, 0.1% (v/v) Triton X-100, and 50% (v/v) glycerol.

Storage
 
Store at -20°C in a frost-free freezer. Multiple freezing and thawing of RNA should be avoided. Keep RNA on ice all the time. It is recommended that the first strand cDNA synthesis is carried out under conditions where RNase contamination has been eliminated.

General Protocol

RT-PCR reactions should be assembled in a RNA-free environment. The use of "clean", automatic pipettes designated for PCR and aerosol resistant barrier tips are recommended.

1. Thaw template RNA and all reagents on ice. Mix each solution by vortexing, and centrifuge briefly to collect residual liquid from the sides of the tubes. 
2. Prepare the following reaction mixture in a PCR tube on ice:

3. Incubate at 25°C for 10 minutes if random primer is used. Omit this step if Oligo(dT) primer or sequence specific primer are used.
4. Incubate the mixture at 42°C for 60 minutes.
5. Stop the reaction by heating at 85°C for 5 minutes. 
6. Chill on ice. The newly synthesized first-strand cDNA now can be used directly for PCR amplification.

Notes:
1. Isolation of poly(A)+ RNA from total RNA is not mandatory; however, doing so may improve the yield and purity of the final product. 
2. RNA sample must be free of contaminating genomic DNA.
3. Unlike the oligo(dT) priming, which usually requires no optimization, the ratio of a randomprimer to RNA is critical in terms of the average length of cDNA synthesized in the reaction. Increasing the ratio of random primer/RNA will result in higher yield of shorter (~500 bp) cDNA, whereas decreasing this ratio will produce longer products.

4. The synthesized cDNA should be stored at -20°C.