PCR/qPCR

MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more.  If you are looking for any of these things, we can match almost all needs to all cyclers.  If you don’t find it easily contact us at tech@midsci.com and we will find it for you. 

96 or 384 we have it.  Non-Skirted, semi-skirted or full skirted, we have it.  0.1, 0.2, or 0.5 mL we have it.  A1, A12, H12, A24, P24 cuts, we have them.  Sybr© Green or probe alternative, we have it.  

Pro Tip: Colored plastics do not have any impact on DNA amplification.  But when setting up qPCR it is often recommended to use white plastics.  This will limit or prevent fluorescence refraction.  Light refraction can minimize sensitivity and consistency. 

Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips.  This will help optimize and reduce variance in the reactions.  Simple test, see the residual liquid left in tips?  This means some of the taq, dNTPs, sample, etc is left in the tip.  PR1MA tips help reduce that significantly and improve your consistency. 

Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate?  Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler.  Call us we have solutions.

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Accuris qMAX Probe Bulk Packaging

Optimized for use with TaqMan™, Scorpions® and molecular beacon probes, qMax Probe qPCR Mix is a ready-to-use formulation for real time quantitative assays.

Same high efficiency for multiplex and singleplex reactions.
  • Includes Accuris Hot Start Taq Polymerase for greater specificity and accuracy.
  • Compatible with popular hydrolysis and beacon probes.
  • Ready to use 2x mastermix.
  • Early Ct values and detection across a broad dynamic range
Optimized for use with TaqMan™, Scorpions® and molecular beacon probes, qMax Probe qPCR Mix is a ready-to-use formulation for real time quantitative assays. qMax Probe utilizes Accuris Hot Start Taq Polymerase or robust PCR with a variety of templates. A specially formulated buffer provides optimal conditions for both superior polymerase function and probe detection, resulting in earlier Ct values and a broad detection range. Complicated, multiplex reactions can be performed without any loss in performance or decrease in detection. The 2X mix requires little, if any optimization and can be used with both fast and standard protocols.
 
qMax Probe qPCR Mix can be used to detect any DNA template, including genomic DNA and cDNA. Available in high, low and no ROX formulations, qMax Probe is compatible with most qPCR instruments.

Bulk Packaging:
To accommodate the requirements of high throughput users, Accuris is now  offering their full line of qPCR and RT-qPCR reagents in bulk packaging at highly discounted prices. Bottle sizes of 25 mL (2500 reactions) and 50 mL (5000 reactions) are available for qMAX SYBR Green and qMAX Probe mixes. One Step RT-qPCR kits are also available in bulk format for 2500 and 5000 reactions and are ideal for labs performing pathogen detection. 

Please Note:
Low Rox and High Rox formulations are available for compatibility with all brands of qPCR cyclers.  Please specify when ordering.  
All PCR reagents ship in protective coolers with frozen gel packs. 

 

Accuris E3200 UV Transilluminator

 

Accuris UV Transilluminators feature space-saving designs, to fit perfectly in any busy laboratory. Three models are available, with a range of intensity settings and viewing surface sizes. Choose your model depending on your application and the size or number of gels to analyze.

 

The E3200 offers a large viewing surface of 21 x 26 cm to accommodate larger gels or multiple small gels. High powered, 14W UV bulbs provide higher sensitivity, so the smallest bands of stained nucleic acids can be visualized. The E3200 also features a control knob for continuous adjustment of the UV intensity.

 

All Accuris UV Transilluminators incorporate scratch-resistant, UV filter glass. Peak output emission of 302 nm is ideal for excitation of gels stained with Ethidium Bromide, SmartGlow, SYBR Green and other common gel stains.

Acrylic UV blocking covers allow safe viewing of samples, and friction hinges on the front edge allow positioning of the cover at an angle for gel access.   


Specifications
 
ModelE3200 UV Transilluminator
Light Source4 x 14W UV Bulbs
Exterior Dimensions (W x D x H)13.6 x 11.8 x 3.5 in  | 34.5 x 30 x 9 cm
Electrical24VDC (AC power adapter is included)
Output WavelengthPeak at 302 nm
Viewing Surface21 x 26 cm | 8.27 x 10.24 in
Weight2.3 kg | 5.15 lbs
Warranty2 years

Item#:
ASACCUUVTRANUL2

Accuris qMAX One-Step RT-qPCR kits

  • RNA to cDNA to qPCR, in one tube
  • High purity enzyme formulation for enhanced stability and performance
  • Accuris Hot-Start Taq allows for preparation at room-temperature
  • Blue dye facilitates pipetting and visualization in plates
  • Available for green fluorescence or probe detection
  • Multiplex formulation available for multiple target amplification
  • Bulk pricing available for high throughput labs and kit manufacturers contact us for details

Accuris qMAX™ One Step Kits allow for highly sensitive real time RT-qPCR assays to be performed directly from RNA templates. Workflows are simplified with optimized formulations of ready-to-use 2X qPCR master mix and 20X reverse transcriptase.

Optimized buffer includes powerful RNase inhibitors, and an extremely thermostable MMLV-derived reverse transcriptase enables robust first strand cDNA synthesis. Accuris Hot Start Taq uses an antibody mediated hot start mechanism allowing for sample preparation at room temperature. Only after an initial incubation at 95C will the Taq become active, so non-specific amplification is greatly reduced. An inert blue dye is included in the Taq master mix to help simplify pipetting and reduce errors.

Three versions of our One Step qPCR kits are available:

qMAX Green One Step kits incorporate our proprietary intercalating dye which exhibits higher fluorescent and lower PCR inhibition than other popular dyes such as SYBR.

qMAX Probe One Step kits are optimized for use with popular TaqMan, Scorpions, and molecular beacon probes.

qMAX Probe One Step Multiplex kits are specifically developed and optimized for efficient probe-based detection of multiple targets in a single reaction well. The Multiplex formulation is comprised of a 20x reverse transcriptase and 2x PCR Mix preparation ideally suited for complex RNA samples including low-copy number viral RNA commonly used in the clinical and research laboratory. qMAX Probe One Step Multiplex kits have been designed to overcome the many challenges of multiplex RT-PCR, by addressing important factors such as the balance between magnesium chloride and deoxynucleotide concentrations, the relative Taq Polymerase and reverse transcriptase concentration, and the ionic conditions of the core reaction buffer.

All Accuris One-Step Kits are compatible with standard and fast cycling protocols and provide increased sensitivity, speed, and reproducibility for a broad range of samples and targets. The polymerase mix is available with different levels of ROX reference dye for compatibility with all qPCR instruments.

Limited Time Promo!


LabNet LabValues Promotions 2024!

For a limited time enjoy discounted pricing on the ENDURO™ Power Supply Series (E0303 and E0203)


See flyer for more details. 

 

Promo is valid 07/1/24 – 12/31/24.

Not to be combined with any offers or discounts. No institutional pricing. All terms apply.   


ENDURO™ Power Supply 

 

  • Two models (300V and 250V) available
  • For DNA, RNA, or protein electrophoresis, and blotting applications
  • Constant voltage or constant current with automatic crossover
  • 250V model also offers a constant power operating mode
  • Timed or continuous operation
  • Short circuit and over current/ over voltage protection
  • No load and load change detection system
  • Programming includes option for automatic recovery after power failure
  • 3 year warranty

 

The new Enduro Power Supplies meet a wide range of applications including DNA, RNA, and protein electrophoresis, blotting, and many others. Two models are available with voltage ranges to 300V and current ranges to 3000mA. Operating modes include constant voltage or constant current with automatic crossover. In addition, the 250V model offers a constant power operating mode.

 

All parameters, including voltage, current, power (250V model only), and time are easily set using the membrane keypad and displayed digitally on the back lit LCD. Compact in design with a small footprint, the Enduro Power Supplies are also stackable to save space. For easy viewing and setting of parameters on the bench or on a shelf, the front of the power supply can be raised with the integral folding feet.

Specifications

Model300V250V
Output Voltage Range/Increments2-300V/1V5-250V/1V
Output Current Range/Increments4-500mA/1mA10-3000mA/10mA
Output Power Range/Increments90 watts max/n.a.1-300W/1W
Operating Constant Modescurrent or voltagecurrent, voltage, or power
Timer1 -99 min, or continuous1min. to 99h 59min or continuous
ProgrammablenoYes (20 program capacity)
Dimensions (W x D x H)19 x 25 x 8 cm19 x 25 x 8 cm
Weight2.6 lbs5.7 lbs
Input Voltage110-230 V110-230 V
Item#:
ASPOWERSUP1

LabNet LabValues Promotions 2024!

 

For a limited time enjoy discounted pricing on select Labnet Enduro™ UV Transilluminators

 

See flyer for more details.

 

Promo is valid 07/1/24 – 12/31/24.

Not to be combined with any offers or discounts. No institutional pricing. All terms apply.

Enduro™ UV Transilluminators


The NEW ENDURO™ UV Transilluminator represents a great new imaging product true to ENDURO™ name. This UV transilluminator has been designed for rugged use and durability with exceptional performance.
  • Compact size with 21 x 26 cm viewing area
  • Available in single or dual wavelength models
  • Unique heavy duty hinge design can be held in place at any angle for gel cutting
  • UV shield fully protects user from UV light
  • Uniform UV Black Glass provides improved imaging contrast
 
The use of UV Black Glass minimizes the striping effect seen on many UV transilluminators. This provides a more uniform background for gel imaging
Item#:
ASENDUROUVTRANS

PurePlus® PCR Plates

  • PurePlus® plates are tested for RNase/DNase lot by lot to ensure pure product
  • Our plates are lot certified free of Human DNA contamination
  • Our plates are tested for ATP lot by lot to ensure pure consistent product
  • Broad product range. Over 18 styles of plates fit most major thermocyclers
  • These plates are autoclavable to 122° C for 15 minutes at 15PSI

Ultra Clear and Ultra Clean
We make our PurePlus® Polymerase Chain Reaction (PCR) consumables without additives so you don't need to wonder what is different about the plates, tubes, or strips you are using this week. We also test every batch for contamination by Human DNA as well as the more common tests that other suppliers offer for RNase and DNase enzymes. Many of our plates are printed with black alpha numeric reference grids to make it easier for you to keep track of your samples. We also offer optically clear caps and opaque black or white plates for Real Time PCR. And we also offer re-usable silicone sealing mats and polypropylene sealing film. Labcon PurePlus® PCR tubes, strips and plates are designed to fit many popular thermocyclers including brands like Applied Biosystems, Eppendorf, Thermo Scientific, and Bio Rad. All our PCR disposables are made from 100% virgin polypropylene and are lot tested and certified to be free of RNase, DNase, and Human DNA. Autoclavable. Our pure resin consumables are available in strips, individual tubes, plates in clear natural or colors.

Our PurePlus® sterile disposables, like all our other sterile products, receive valid sterilization processing compliant with ISO 11137. So unlike "pre-sterile" products from other suppliers you can use PurePlus® PCR disposables for diagnostic and regulated procedures.
 

MIDSCI # VWR #
3983-520-000-9 82006-678
3983-520-010-9 89218-288
3984-520-000-9 89218-294
3961-520-000-9 89218-296
3962-520-000-9 89218-298
3962-521-000-9 10755-256
3971-520-000-9 82006-650
3976-520-000-9 82006-664
3978-520-000-9 82006-704
3978-520-010-9 89218-292
3978-522-000-9 82006-714
3978-524-000-9 82006-710
3978-526-000-9 82006-712
3978-527-000-9 82006-706
3978-528-000-9 82006-708
3978-529-000-9 82006-718
3963-520-000-9 83009-676
3967-520-000-9 83009-668
3968-520-000-9 83009-672
3972-520-000-9 89049-178
3972-520-010-9 89218-290
3973-520-000-9 83007-374
3973-529-000-9 83007-372
3974-520-000-9 37001-272
3977-520-000-9 82006-636
3977-522-000-9 82006-646
3977-526-000-9 82006-644
3977-527-000-9 82006-638
3977-528-000-9 82006-640
3977-529-000-9 82006-648
3979-520-000-9 93001-100
Item#:
ASLABPUREPLS
96x0.1ml, Sub-Skirted,
This item has been discontinued and replaced by item 
  • Plate Characteristics
    • 96 x 0.1mL, Sub-skirted
    • Low Profile (0.1mL), Frosted (low signal to noise ratio
    • Extreme Uniformity (EU) Plate Design
    • Uniform wall thickness ensures consistent even heating
    • Thin wall-bottomed tube for rapid heat transfer
  • Ideal paring with EU Caps with "Sealing Ring Band" Design
    • Easy open - easy close with ergonomic caps: B57801, B79701, C79701
    • Caps are significantly easier to apply and remove -- Repetitive Strain Injury (RSI) Friendly.
    • Significant reduction in evaporation due to a wider sealing area.
  • Certified DNA, DNase, RNA, RNase and pyrogen-free

Please give us a call for a sample!

 

Order#

Description

Non-Skirt

 

B70501

Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, Natural, 25/cs

B70509

Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, White, 25/cs

B50501

Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, Frosted, 25/cs

AB70651

Pryme PCR Plate, 96-Well, 0.1mL, Non-Skirt, Low Profile, Frosted, 25/cs

Semi-Skirted

 

B70651

Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, Natural, 25/cs

B70659

Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, White, 25/cs

B50651

Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, Frosted, 25/cs

B17480

Pryme PCR Plate, 96-Well, 0.1mL, Semi-skirted, Low Profile, Frosted, 25/cs

B17489

Pryme PCR Plate, 96-Well, 0.1mL, Semi-skirted, Low Profile, White, 25/cs

Sub-Skirted

 

ABI7501

Pryme PCR Plate, 96-Well, 0.2mL, Sub-skirted, Regular Profile, Frosted, 25/cs

ABI7509

Pryme PCR Plate, 96-Well, 0.2mL, Sub-skirted, Regular Profile, White, 25/cs

AB19800-EU

Pryme PCR Plate, 96-Well, 0.1mL, Sub-skirted, Low Profile, Frosted, 25/cs

AB19809-EU

Pryme PCR Plate, 96-Well, 0.1mL, Sub-skirted, Low Profile, White, 25/cs

Caps

 

B57801

Pryme PCR Ergonomic 8-Strip, Optical Flat (V2) Caps, Natural, 120/cs

B79701

Pryme PCR Ergonomic 8-Strip, Optical Flat (V1) Caps, Natural, 120/cs

C79701

Pryme PCR Ergonomic 8-Strip, Semi-Domed (V1) Caps, Natural, 120/cs

Item#:
AB19809-EU
Your Price:
158.24
Each
This item is discontinued

Plate-Sealing Roller

Application: Plate-sealing
Material: Rubber, metal

The plate-sealing roller features a smooth spinning, thermoplastic elastomer sealing surface with a durable plated metal handle.

  • Reusable, autoclavable
  • Provides a large 4 inch (10.16 cm) wide sealing surface
  • A 10 minute or greater dwell me at room temperature before processing increases the film-to-plate bond
Skirt, PCR, Clear, 10/pk
This item has been discontinued and replaced by item 
Please know that the manufacturer has suspended manufacturing this product indefinitely.
We suggest purchasing 
PR1MA PCR Plastics.
Contact your rep or email tech@midsci.com for more information.


 
  • Standard thermal cycling and qPCR compatible
  • Ultra-thin and consistent wall thickness for precise thermal transfer
  • Automation friendly, single notch corner, stackable
  • Made from 99.9% virgin polypropylene
  • 240ul well capacity (working capacity dependent on closure method)
  • Barcoding available
  • Certified DNAse/RNAse and pyrogen-safe
Item#:
PCR-96-FS-C
Your Price:
60.39
Each
This item is discontinued
Item#:
ASPCR0P2
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