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PCR/qPCR

MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more.  If you are looking for any of these things, we can match almost all needs to all cyclers.  If you don’t find it easily contact us at tech@midsci.com and we will find it for you. 

96 or 384 we have it.  Non-Skirted, semi-skirted or full skirted, we have it.  0.1, 0.2, or 0.5 mL we have it.  A1, A12, H12, A24, P24 cuts, we have them.  Sybr© Green or probe alternative, we have it.  

Pro Tip: Colored plastics do not have any impact on DNA amplification.  But when setting up qPCR it is often recommended to use white plastics.  This will limit or prevent fluorescence refraction.  Light refraction can minimize sensitivity and consistency. 

Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips.  This will help optimize and reduce variance in the reactions.  Simple test, see the residual liquid left in tips?  This means some of the taq, dNTPs, sample, etc is left in the tip.  PR1MA tips help reduce that significantly and improve your consistency. 

Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate?  Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler.  Call us we have solutions.

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Accuris qMAX Probe Bulk Packaging

Optimized for use with TaqMan™, Scorpions® and molecular beacon probes, qMax Probe qPCR Mix is a ready-to-use formulation for real time quantitative assays.

Same high efficiency for multiplex and singleplex reactions.
  • Includes Accuris Hot Start Taq Polymerase for greater specificity and accuracy.
  • Compatible with popular hydrolysis and beacon probes.
  • Ready to use 2x mastermix.
  • Early Ct values and detection across a broad dynamic range
Optimized for use with TaqMan™, Scorpions® and molecular beacon probes, qMax Probe qPCR Mix is a ready-to-use formulation for real time quantitative assays. qMax Probe utilizes Accuris Hot Start Taq Polymerase or robust PCR with a variety of templates. A specially formulated buffer provides optimal conditions for both superior polymerase function and probe detection, resulting in earlier Ct values and a broad detection range. Complicated, multiplex reactions can be performed without any loss in performance or decrease in detection. The 2X mix requires little, if any optimization and can be used with both fast and standard protocols.
 
qMax Probe qPCR Mix can be used to detect any DNA template, including genomic DNA and cDNA. Available in high, low and no ROX formulations, qMax Probe is compatible with most qPCR instruments.

Bulk Packaging:
To accommodate the requirements of high throughput users, Accuris is now  offering their full line of qPCR and RT-qPCR reagents in bulk packaging at highly discounted prices. Bottle sizes of 25 mL (2500 reactions) and 50 mL (5000 reactions) are available for qMAX SYBR Green and qMAX Probe mixes. One Step RT-qPCR kits are also available in bulk format for 2500 and 5000 reactions and are ideal for labs performing pathogen detection. 

Please Note:
Low Rox and High Rox formulations are available for compatibility with all brands of qPCR cyclers.  Please specify when ordering.  
All PCR reagents ship in protective coolers with frozen gel packs. 

 
Item#:
ASACQMAXPROBE
 
PR2001 qMax Probe All Product Insert

Accuris E3200 UV Transilluminator

Accuris UV Transilluminators feature space-saving designs, to fit perfectly in any busy laboratory. Three models are available, with a range of intensity settings and viewing surface sizes. Choose your model depending on your application and the size or number of gels to analyze. 

The E3200 offers a large viewing surface of 21 x 26 cm to accommodate larger gels or multiple small gels. High powered, 14W UV bulbs provide higher sensitivity, so the smallest bands of stained nucleic acids can be visualized. The E3200 also features a control knob for continuous adjustment of the UV intensity.

 

All Accuris UV Transilluminators incorporate scratch-resistant, UV filter glass. Peak output emission of 302 nm is ideal for excitation of gels stained with Ethidium Bromide, SmartGlow, SYBR Green and other common gel stains.

Acrylic UV blocking covers allow safe viewing of samples, and friction hinges on the front edge allow positioning of the cover at an angle for gel access.      

Specifications
 
ItemE3200 UV Transilluminator
Light Source4 x 14W UV Bulbs
Exterior Dimensions (W x D x H)13.6 x 11.8 x 3.5 In.  | 34.5 x 30 x 9 cm
Electrical24VDC (AC power adapter is included)
Output WavelengthPeak at 302 nm
Viewing Surface8.27 x 10.24 In. | 21 x 26 cm
Weight5.15 lbs. | 2.3 kg.
Warranty2 years

Item#:
ASACCUUVTRANUL2

Accuris qMAX One-Step RT-qPCR kits

  • RNA to cDNA to qPCR, in one tube
  • High purity enzyme formulation for enhanced stability and performance
  • Accuris Hot-Start Taq allows for preparation at room-temperature
  • Blue dye facilitates pipetting and visualization in plates
  • Available for green fluorescence or probe detection
  • Multiplex formulation available for multiple target amplification
  • Bulk pricing available for high throughput labs and kit manufacturers contact us for details

Accuris qMAX™ One Step Kits allow for highly sensitive real time RT-qPCR assays to be performed directly from RNA templates. Workflows are simplified with optimized formulations of ready-to-use 2X qPCR master mix and 20X reverse transcriptase.

Optimized buffer includes powerful RNase inhibitors, and an extremely thermostable MMLV-derived reverse transcriptase enables robust first strand cDNA synthesis. Accuris Hot Start Taq uses an antibody mediated hot start mechanism allowing for sample preparation at room temperature. Only after an initial incubation at 95C will the Taq become active, so non-specific amplification is greatly reduced. An inert blue dye is included in the Taq master mix to help simplify pipetting and reduce errors.

Three versions of our One Step qPCR kits are available:

qMAX Green One Step kits incorporate our proprietary intercalating dye which exhibits higher fluorescent and lower PCR inhibition than other popular dyes such as SYBR.

qMAX Probe One Step kits are optimized for use with popular TaqMan, Scorpions, and molecular beacon probes.

qMAX Probe One Step Multiplex kits are specifically developed and optimized for efficient probe-based detection of multiple targets in a single reaction well. The Multiplex formulation is comprised of a 20x reverse transcriptase and 2x PCR Mix preparation ideally suited for complex RNA samples including low-copy number viral RNA commonly used in the clinical and research laboratory. qMAX Probe One Step Multiplex kits have been designed to overcome the many challenges of multiplex RT-PCR, by addressing important factors such as the balance between magnesium chloride and deoxynucleotide concentrations, the relative Taq Polymerase and reverse transcriptase concentration, and the ionic conditions of the core reaction buffer.

All Accuris One-Step Kits are compatible with standard and fast cycling protocols and provide increased sensitivity, speed, and reproducibility for a broad range of samples and targets. The polymerase mix is available with different levels of ROX reference dye for compatibility with all qPCR instruments.

Item#:
PNQMAXMULTI
 
qMAXProbe Multiplex
PR2120 qMax Green One-Step RT-qPCR Product Insert
PR2121 One-Step Probe qPCR Product Insert
PR2122 Product Insert
Limited Time Promo!

LabNet LabValues Promotions 2024!

For a limited time enjoy discounted pricing on the AccuSeal Semi-Automated Plate Sealer (PS1000)
 

 See flyer for more details.

 

Promo is valid 07/1/24 – 12/31/24.

Not to be combined with any offers or discounts. No institutional pricing. All terms apply.   



AccuSeal Semi-Automated Plate Sealer

 

AccuSeal Semi Automated Plate Sealers from MIDSCI are engineered and constructed by Labnet International, Inc to maximize PCR, assay, and storage applications with a user-friendly panel and automated sealing options.


Great emphasis has been placed on incorporating a wide range of standard heights to deep well microplates with appropriate adaptors to efficiently fit the plates for consistent temperature regulation. The AccuSeal sealer is optimized for simple digital control and operation, user-friendly versatility and convenience as well as safety.
 

Controls and Operation:

  • Intuitive layout permits optimal sealing time, temperature and settings
  • Includes a "No Heat" setting for pressure sealing applications

Versatility and Convenience:

  • Adaptors specific for a PCR plate or an assay plate accommodate a variety of fits
  • Automatically adjusts heat for different height plates
  • Provides uniform pressure for a quality seal with a special non-stick coating to prevent messy cleanup.

 

Safety:

  • Motorized drawer features safety switches and prevents injury to the user.


Specifications
 
Dimensions6.75x12.75x14.25 inches
Weight24 Lbs
Sealing TemperatureOFF, 100 to 190 °C
Temperature Accuracy+/- 1.00 °C
Temperature Uniformity+/- 1.00 °C
Sealing Time0.5 to 10 sec
Compatible Plate MaterialsPP (Polypropylene)
PS (Polystyrene)
PE (Polyethylene)
Compatible Plate TypesStandard Assay Plates
Deep-well Storage Plates
PCR Plates: skirted, semi-skirted, non-skirted
Maximum Plate Height45mm
Compatible Sealing Film TypesFoil-polypropylene laminate
Clear polyester-polypropylene laminate
Clear polymer
Thin clear polymer
Foil-laminate
Foil

Adapters 
 
PS1000-PCRAccuSeal PCR plate adapter for sealing PCR and 384 well plates
PS1000-ADAPTAccuSeal Assay plate adapter for sealing assay plates

Sealing Film

PS1000-150AccuSeal Sealing Heat Sealing Film, Ultra Clear, Peelable, qPCR, 100 films/bx, 5 bx/cs
PS1000-200AccuSeal Sealing Heat Sealing Film for Low Temp, Peelable, PCR, 100 films/bx, 5 bx/cs
PS1000-300AccuSeal Sealing Heat Sealing Film for Storage, Piercable, Aluminum,100 films/bx, 5 bx/cs
PS1000-400AccuSeal Sealing Heat Sealing Film for Compound Storage, Peelable, 100 films/bx, 5 bx/cs
PS1000-GP300AccuSeal Sealing Heat Sealing Film w/ Paper Back, General, PCR, 100 films/bx, 10 bx/cs
PS1000-GP111AccuSeal Sealing Heat Sealing Film, Easy Pierce, 100 films/bx, 5 bx/cs

 

Item#:
ASPCRFILM8

SmartSeal™ Semi-Automated Microplate Sealer

The Accuris SmartSeal™ Automated Plate Sealer provides fast, reproducible sealing of microplates. Unlike manual plate sealing, the SmartSeal ensures uniform sealing of all wells and consistency from one plate to the next.
  • Fast, reproducible sealing of well plates
  • Adjustable time, temperature and pressure for optimized sealing
  • User friendly controls, load plate and press run to seal
  • Accepts a wide range of plate types and heights, including deep well storage plates, assay plates, pcr plates, etc.
The Accuris SmartSeal™ Automated Plate Sealer provides fast, reproducible sealing of microplates. Unlike manual plate sealing, the SmartSeal ensures uniform sealing of all wells and consistency from one plate to the next.

With digital control over all parameters (time, temperature and pressure) the SmartSeal is capable of accepting a wide range of plate types and heights. An aluminum block adapter is included (for assay and PCR plates) increasing the compatibility of the instrument to allow for sealing nearly any plate up to 48mm tall.

With sealing times as low as 5 seconds, the SmartSeal is a time saving solution for labs and production facilities requiring high quality and consistent plate sealing.

Compatible with:

  • Deep well (storage) plates up to 48mm tall
  • Assay plates
  • PCR Plates (skirterd, semi-skirted and non-skirted)
  • Polypropylene polystyrene or Polyethylene plates
  • Foil or polymer (transparent) seals

Specifications:
 
  Model  Accuris SmartSeal
  Temp. Range  80°C to 200°C
  Temp. Accuracy  1°C
  Sealing Time  0.5 sec. to 10 sec. (0.1 sec. increments)
  Max. Plate Height  48 mm
  Dimensions  17.8 x 37 x 33 cm / 7 x 14.6 x 13 in.
  Weight  9.5 kg / 21 lbs
  Electrical 115V or 230V, 50-60Hz, 300W
Item#:
ASSMARTSEALTM
 
MS1000 SmartSeal PDF
Convenient, Easy-to-Use Roll format

SealPlate® RollMate™ Pre-scored Film in a Convenient, Easy to Use Roll Format


Pre-scored film on a roll is a more economical and efficient alternative to rapidly seal microplates or racked test tubes; faster than applying single sheeted film. Self-standing, non-slip supports hold the pre-scored film roll securely in place for quick and easy bench-top dispensement. The roll unwind tension can be easily adjusted to user preference. Continuous backing liner can be torn off or fed directly into a waste basket for disposal.
  • Economical
  • Quicker, more efficient sealing
  • Provides a secure seal and is easy to remove
  • Rigid polyester film features good clarity and easy handling
  • Can be used as a temporary seal while awaiting further processing
Applications
  • General laboratory use
  • Short-term storage
  • Incubation
  • ELISA
Temperature Range
  • -40°C to +120°C
Item#:
ASFILMROLLMATE
 
SealPlate® RollMate Features
Specs SealPlate® RollMate
Sealing film selection guide by application
Sealing film selection guide by attributes

EZcap™FoilStrips


An economical and more user-friendly alternative to PCR strip caps; no sore thumbs and fingers guaranteed!

Application: PCR and light sensitive assays
Temperature Range: -20°C to +120°C

Precision cut to a smaller dimension for sealing one PCR 8-tube strip or a single row of a 96-well PCR or deep-well storage plate whenever rows must be
selectively protected or accessed. FoilStrips remove easily with no splash back as can occur with strip-caps.

  • Certified DNase- & RNase- free
  • Each sheet contains 6 strips on a continuous liner for ease of use
  • Pierceable with a pipet tip or robotic probe for direct sample recovery
  • The conformable nature of foil allows for the visual verification of the seal’s security around each well
  • 2.0mil aluminum foil removes easily without tearing
 
Helpful Tips:

  • A non-locking workstation is recommended for sample prep (e.g. 96-well round-boom plate)
  • Removal of the end-tabs at the perforations is recommended before cycling
  • A final sealing step is highly recommended in the thermal cycler because 8-tube strips flex during handling
  • Do not fold foil around sides of tubes
Item#:
ASEZCAFOIL
 
EZcapFoilStrips Features
Specs EZcapFoilStrips
Sealing film selection guide by application
Sealing film selection guide by attributes

AlumaSeal® CS™


With a 50 µm layer of acrylic adhesive that withstands cold storage at temperatures to -80 °C.

Unlike other sealing films in the AlumaSeal group, AlumaSeal CS sealing films are not recommended for PCR or thermocycling. A single non-perforated end tab simplifies application. Simply hold the tab and strip the backing from the body of the sealing film as it lays on the plate to avoid curling. Dimensions are 82.6 by 132.6 mm, including the single 9.5 mm end tab. AlumaSeal CS sealing films are certified DNase-, RNase-, and nucleic-acid-free.

  • Specially formulated for cold storage to -80 °C
  • Excellent barrier properties to retard evaporation
  • Easily pierceable with pipet tips or robotic probes for sample recovery
 
*Completing application of AlumaSeal CS sealing films by pressing with the accessory plate roller assures a secure uniform seal around all wells.
Item#:
ASALUMASEL
 
AlumaSeal CS Features
Specs AlumaSeal CS
Sealing film selection guide by application
Sealing film selection guide by attributes
500 Pre-Scored Sheets

FoilSeal™ RollMate™ Dispenser +1 Roll of FoilSeal™ Film, 500 Pre-Scored Sheets

Pre-scored film on a roll is a more economical and efficient alternative to rapidly seal microplates; faster than applying single sheeted film. Self-standing, non-slip supports hold the pre-scored film roll securely in place for quick and easy bench-top dispensement. The roll unwind tension can be easily adjusted to user preference. Continuous backing liner can be torn off or fed directly into a wastebasket for disposal.
  • Certified DNase- & RNase- free
  • Pierceable with a pipet tip or robotic probe for direct sample recovery
  • 2.0mil aluminum foil removes easily without tearing
  • The conformable nature of foil allows for the visual verification of the seal’s security around each well
  • Not recommended for long-term cold storage
Applications
  • PCR
  • Light sensitive assays
  • Short-term storage
  • Robotics
Temperature Range
  • -20°C to +120°C
Helpful Tips
  • End-tab removal before thermal cycling is highly recommended
  • Remove diagonally from a corner to prevent tearing

Item#:
ASFOILSEALROLLMATE
 
FoilSeal RollMate Features
Specs FoilSeal RollMate
Sealing film selection guide by application
Sealing film selection guide by attributes
Soft, Extra Thick, Conformable Adhesive

ThermalSeal® 2™ and ThermalSeal® Advanced Sealing Film

ThermalSeal® 2™ and ThermalSeal® Advanced feature a soft, extra thick, conformable adhesive that forms a very strong seal around raised wells to minimize evaporation and cross-contamination.
  • Certified DNase- & RNase- free
  • Ideal for use with flat-top, semi-skirted and full-skirted PCR plates
  • Compatible with hard-shell dual component (e.g. polycarbonate/polypropylene) PCR plates
  • Large cut allows for easier positioning of the film to the plate via edge of plate alignment vs. centering
  • Available in a smaller cut for raised-rim and non-skirted PCR plates: ThermalSeal® Advanced
Application
  • PCR
Temperature Range
  • -40°C to +120°C
Helpful Tips
  • Selection of the proper film size will minimize evaporation
  • The film should not overhang or ride up the wall of a raised-rim plate
  • More complete coverage of skirted flat-top plates will reduce evaporation in the outer wells
  • End-tab removal before thermal cycling is highly recommended

Item#:
ASTHERMSEAL2A
 
Specs ThermalSeal 2
Sealing film selection guide by application
Sealing film selection guide by attributes
ThermalSeal 2 Features

Zone-Free™


Zone-Free is a multilayer film consisting of a top pierceable clear polyethylene film and a white polypropylene sublayer with 96 circular areas void of adhesive.

Application: Short-term storage and robotics
Temperature Range: -40°C to +90°C

The white sublayer aids in the alignment of the film to the plate; alignment of opposing corner wells ensures that all 96 wells will be properly aligned. The adhesive-free zones are 9mm on center to facilitate multi-channel pipetting without adhesive fouling of pipet tips or robotic probes.

  • Pierceable
  • Offers excellent chemical resistance
  • Samples have minimal contact with adhesive due to the adhesive-free zones
Item#:
ASZONEFREFIL
 
Sealing film selection guide by application
Sealing film selection guide by attributes
Zone-FreeFilm Features
Specs Zone-FreeFilm
An Easier to Remove Film

ThermalSeal® An Easier to Remove Sealing Film

ThermalSeal® features a moderate strength adhesive for ease of removal.

  • Certified DNase- & RNase- free
  • Only recommended for structurally rigid polypropylene PCR plates that don’t warp
  • A less rigid polypropylene film that minimizes aerosols upon removal
  • Features good chemical resistance
Application
  • PCR
  • General storage
Temperature Range
  •  -40°C to +120°C

Item#:
ASTHERMALSEAL
 
ThermalSeal Features
Specs ThermalSeal
Sealing film selection guide by application
Sealing film selection guide by attributes
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