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ActionsPCR/qPCR
MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more. If you are looking for any of these things, we can match almost all needs to all cyclers. If you don’t find it easily contact us at tech@midsci.com and we will find it for you.
96 or 384 we have it. Non-Skirted, semi-skirted or full skirted, we have it. 0.1, 0.2, or 0.5 mL we have it. A1, A12, H12, A24, P24 cuts, we have them. Sybr© Green or probe alternative, we have it.
Pro Tip: Colored plastics do not have any impact on DNA amplification. But when setting up qPCR it is often recommended to use white plastics. This will limit or prevent fluorescence refraction. Light refraction can minimize sensitivity and consistency.
Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips. This will help optimize and reduce variance in the reactions. Simple test, see the residual liquid left in tips? This means some of the taq, dNTPs, sample, etc is left in the tip. PR1MA tips help reduce that significantly and improve your consistency.
Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate? Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler. Call us we have solutions.
Accuris E3200 UV Transilluminator
Accuris UV Transilluminators feature space-saving designs, to fit perfectly in any busy laboratory. Three models are available, with a range of intensity settings and viewing surface sizes. Choose your model depending on your application and the size or number of gels to analyze.
The E3200 offers a large viewing surface of 21 x 26 cm to accommodate larger gels or multiple small gels. High powered, 14W UV bulbs provide higher sensitivity, so the smallest bands of stained nucleic acids can be visualized. The E3200 also features a control knob for continuous adjustment of the UV intensity.
All Accuris UV Transilluminators incorporate scratch-resistant, UV filter glass. Peak output emission of 302 nm is ideal for excitation of gels stained with Ethidium Bromide, SmartGlow, SYBR Green and other common gel stains.
Acrylic UV blocking covers allow safe viewing of samples, and friction hinges on the front edge allow positioning of the cover at an angle for gel access.
Specifications
Item | E3200 UV Transilluminator |
Light Source | 4 x 14W UV Bulbs |
Exterior Dimensions (W x D x H) | 13.6 x 11.8 x 3.5 In. | 34.5 x 30 x 9 cm |
Electrical | 24VDC (AC power adapter is included) |
Output Wavelength | Peak at 302 nm |
Viewing Surface | 8.27 x 10.24 In. | 21 x 26 cm |
Weight | 5.15 lbs. | 2.3 kg. |
Warranty | 2 years |
Accuris qMAX One-Step RT-qPCR kits
- RNA to cDNA to qPCR, in one tube
- High purity enzyme formulation for enhanced stability and performance
- Accuris Hot-Start Taq allows for preparation at room-temperature
- Blue dye facilitates pipetting and visualization in plates
- Available for green fluorescence or probe detection
- Multiplex formulation available for multiple target amplification
- Bulk pricing available for high throughput labs and kit manufacturers contact us for details
Accuris qMAX™ One Step Kits allow for highly sensitive real time RT-qPCR assays to be performed directly from RNA templates. Workflows are simplified with optimized formulations of ready-to-use 2X qPCR master mix and 20X reverse transcriptase.
Optimized buffer includes powerful RNase inhibitors, and an extremely thermostable MMLV-derived reverse transcriptase enables robust first strand cDNA synthesis. Accuris Hot Start Taq uses an antibody mediated hot start mechanism allowing for sample preparation at room temperature. Only after an initial incubation at 95C will the Taq become active, so non-specific amplification is greatly reduced. An inert blue dye is included in the Taq master mix to help simplify pipetting and reduce errors.
Three versions of our One Step qPCR kits are available:
qMAX Green One Step kits incorporate our proprietary intercalating dye which exhibits higher fluorescent and lower PCR inhibition than other popular dyes such as SYBR.
qMAX Probe One Step kits are optimized for use with popular TaqMan, Scorpions, and molecular beacon probes.
qMAX Probe One Step Multiplex kits are specifically developed and optimized for efficient probe-based detection of multiple targets in a single reaction well. The Multiplex formulation is comprised of a 20x reverse transcriptase and 2x PCR Mix preparation ideally suited for complex RNA samples including low-copy number viral RNA commonly used in the clinical and research laboratory. qMAX Probe One Step Multiplex kits have been designed to overcome the many challenges of multiplex RT-PCR, by addressing important factors such as the balance between magnesium chloride and deoxynucleotide concentrations, the relative Taq Polymerase and reverse transcriptase concentration, and the ionic conditions of the core reaction buffer.
All Accuris One-Step Kits are compatible with standard and fast cycling protocols and provide increased sensitivity, speed, and reproducibility for a broad range of samples and targets. The polymerase mix is available with different levels of ROX reference dye for compatibility with all qPCR instruments.
Accuris Fast Extraction PCR Kit
The Accuris Fast Extraction PCR Kit features an exclusive single-tube solution that extracts genomic DNA in just 8 minutes. This is swiftly followed by PCR amplification utilizing our High Fidelity Hot Start Red Dye Master Mix, designed for superior endpoint PCR performance. High Fidelity Hot Start Red Dye Master Mix is a 2X formula that incorporates a non-reactive red dye, enabling direct gel loading.
This kit offers versatility to suit different workflows: the PCR-prepared genomic DNA can be directly used in endpoint PCR or can be applied in real-time PCR using either SYBR Green or TaqMan Probe chemistries.
- Offers exceptional versatility and can be used to extract and amplify DNA from a wide range of sample types
- Compatible with animal tissue, plant, saliva, & bacterial samples
- Extraction & Amplification in less than 60 minutes
- Includes High Fidelity Hot Start Red Dye Master Mix to prevent the risk of nonspecific amplification & to visualize bands easily
- Process extracted sample via PCR with included mastermix
- Alternatively, amplify via qPCR (qPCR mastermix not included)
Each 100 reaction kit includes:
1 tube of High Fidelity Hot Start Red Dye Master Mix (1.25 mL)
1 tube of Fast Extraction Lysis Solution (1.25 mL)
Applications: Extraction & Amplification of Tissue, Plant, Saliva, & Bacteria
LabNet LabValues Promotions 2024!
For a limited time enjoy discounted pricing on the ENDURO™ Power Supply Series (E0303 and E0203)
Promo is valid 07/1/24 – 12/31/24.
Not to be combined with any offers or discounts. No institutional pricing. All terms apply.
ENDURO™ Power Supply
- Two models (300V and 250V) available
- For DNA, RNA, or protein electrophoresis, and blotting applications
- Constant voltage or constant current with automatic crossover
- 250V model also offers a constant power operating mode
- Timed or continuous operation
- Short circuit and over current/ over voltage protection
- No load and load change detection system
- Programming includes option for automatic recovery after power failure
- 3 year warranty
The new Enduro Power Supplies meet a wide range of applications including DNA, RNA, and protein electrophoresis, blotting, and many others. Two models are available with voltage ranges to 300V and current ranges to 3000mA. Operating modes include constant voltage or constant current with automatic crossover. In addition, the 250V model offers a constant power operating mode.
All parameters, including voltage, current, power (250V model only), and time are easily set using the membrane keypad and displayed digitally on the back lit LCD. Compact in design with a small footprint, the Enduro Power Supplies are also stackable to save space. For easy viewing and setting of parameters on the bench or on a shelf, the front of the power supply can be raised with the integral folding feet.
Specifications
Model | 300V | 250V |
Output Voltage Range/Increments | 2-300V/1V | 5-250V/1V |
Output Current Range/Increments | 4-500mA/1mA | 10-3000mA/10mA |
Output Power Range/Increments | 90 watts max/n.a. | 1-300W/1W |
Operating Constant Modes | current or voltage | current, voltage, or power |
Timer | 1 -99 min, or continuous | 1min. to 99h 59min or continuous |
Programmable | no | Yes (20 program capacity) |
Dimensions (W x D x H) | 19 x 25 x 8 cm | 19 x 25 x 8 cm |
Weight | 2.6 lbs | 5.7 lbs |
Input Voltage | 110-230 V | 110-230 V |
LabNet LabValues Promotions 2024!
For a limited time enjoy discounted pricing on select Labnet Enduro™ UV Transilluminators
Promo is valid 07/1/24 – 12/31/24.
- Compact size with 21 x 26 cm viewing area
- Available in single or dual wavelength models
- Unique heavy duty hinge design can be held in place at any angle for gel cutting
- UV shield fully protects user from UV light
- Uniform UV Black Glass provides improved imaging contrast
PurePlus® PCR Plates
- PurePlus® plates are tested for RNase/DNase lot by lot to ensure pure product
- Our plates are lot certified free of Human DNA contamination
- Our plates are tested for ATP lot by lot to ensure pure consistent product
- Broad product range. Over 18 styles of plates fit most major thermocyclers
- These plates are autoclavable to 122° C for 15 minutes at 15PSI
Ultra Clear and Ultra Clean
We make our PurePlus® Polymerase Chain Reaction (PCR) consumables without additives so you don't need to wonder what is different about the plates, tubes, or strips you are using this week. We also test every batch for contamination by Human DNA as well as the more common tests that other suppliers offer for RNase and DNase enzymes. Many of our plates are printed with black alpha numeric reference grids to make it easier for you to keep track of your samples. We also offer optically clear caps and opaque black or white plates for Real Time PCR. And we also offer re-usable silicone sealing mats and polypropylene sealing film. Labcon PurePlus® PCR tubes, strips and plates are designed to fit many popular thermocyclers including brands like Applied Biosystems, Eppendorf, Thermo Scientific, and Bio Rad. All our PCR disposables are made from 100% virgin polypropylene and are lot tested and certified to be free of RNase, DNase, and Human DNA. Autoclavable. Our pure resin consumables are available in strips, individual tubes, plates in clear natural or colors.
Our PurePlus® sterile disposables, like all our other sterile products, receive valid sterilization processing compliant with ISO 11137. So unlike "pre-sterile" products from other suppliers you can use PurePlus® PCR disposables for diagnostic and regulated procedures.
MIDSCI # | VWR # |
3983-520-000-9 | 82006-678 |
3983-520-010-9 | 89218-288 |
3984-520-000-9 | 89218-294 |
3961-520-000-9 | 89218-296 |
3962-520-000-9 | 89218-298 |
3962-521-000-9 | 10755-256 |
3971-520-000-9 | 82006-650 |
3976-520-000-9 | 82006-664 |
3978-520-000-9 | 82006-704 |
3978-520-010-9 | 89218-292 |
3978-522-000-9 | 82006-714 |
3978-524-000-9 | 82006-710 |
3978-526-000-9 | 82006-712 |
3978-527-000-9 | 82006-706 |
3978-528-000-9 | 82006-708 |
3978-529-000-9 | 82006-718 |
3963-520-000-9 | 83009-676 |
3967-520-000-9 | 83009-668 |
3968-520-000-9 | 83009-672 |
3972-520-000-9 | 89049-178 |
3972-520-010-9 | 89218-290 |
3973-520-000-9 | 83007-374 |
3973-529-000-9 | 83007-372 |
3974-520-000-9 | 37001-272 |
3977-520-000-9 | 82006-636 |
3977-522-000-9 | 82006-646 |
3977-526-000-9 | 82006-644 |
3977-527-000-9 | 82006-638 |
3977-528-000-9 | 82006-640 |
3977-529-000-9 | 82006-648 |
3979-520-000-9 | 93001-100 |
- Plate Characteristics
- 96 x 0.1mL, Sub-skirted
- Low Profile (0.1mL), Frosted (low signal to noise ratio
- Extreme Uniformity (EU) Plate Design
- Uniform wall thickness ensures consistent even heating
- Thin wall-bottomed tube for rapid heat transfer
- Ideal paring with EU Caps with "Sealing Ring Band" Design
- Easy open - easy close with ergonomic caps: B57801, B79701, C79701
- Caps are significantly easier to apply and remove -- Repetitive Strain Injury (RSI) Friendly.
- Significant reduction in evaporation due to a wider sealing area.
- Certified DNA, DNase, RNA, RNase and pyrogen-free
Please give us a call for a sample!
Order# |
Description |
Non-Skirt |
|
B70501 |
Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, Natural, 25/cs |
B70509 |
Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, White, 25/cs |
B50501 |
Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, Frosted, 25/cs |
AB70651 |
Pryme PCR Plate, 96-Well, 0.1mL, Non-Skirt, Low Profile, Frosted, 25/cs |
Semi-Skirted |
|
B70651 |
Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, Natural, 25/cs |
B70659 |
Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, White, 25/cs |
B50651 |
Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, Frosted, 25/cs |
B17480 |
Pryme PCR Plate, 96-Well, 0.1mL, Semi-skirted, Low Profile, Frosted, 25/cs |
B17489 |
Pryme PCR Plate, 96-Well, 0.1mL, Semi-skirted, Low Profile, White, 25/cs |
Sub-Skirted |
|
ABI7501 |
Pryme PCR Plate, 96-Well, 0.2mL, Sub-skirted, Regular Profile, Frosted, 25/cs |
ABI7509 |
Pryme PCR Plate, 96-Well, 0.2mL, Sub-skirted, Regular Profile, White, 25/cs |
AB19800-EU |
Pryme PCR Plate, 96-Well, 0.1mL, Sub-skirted, Low Profile, Frosted, 25/cs |
AB19809-EU |
Pryme PCR Plate, 96-Well, 0.1mL, Sub-skirted, Low Profile, White, 25/cs |
Caps |
|
B57801 |
Pryme PCR Ergonomic 8-Strip, Optical Flat (V2) Caps, Natural, 120/cs |
B79701 |
Pryme PCR Ergonomic 8-Strip, Optical Flat (V1) Caps, Natural, 120/cs |
C79701 |
Pryme PCR Ergonomic 8-Strip, Semi-Domed (V1) Caps, Natural, 120/cs |
Plate-Sealing Roller
Application: Plate-sealingMaterial: Rubber, metal
The plate-sealing roller features a smooth spinning, thermoplastic elastomer sealing surface with a durable plated metal handle.
- Reusable, autoclavable
- Provides a large 4 inch (10.16 cm) wide sealing surface
- A 10 minute or greater dwell me at room temperature before processing increases the film-to-plate bond
We suggest purchasing PR1MA PCR Plastics.
Contact your rep or email tech@midsci.com for more information.
- Standard thermal cycling and qPCR compatible
- Ultra-thin and consistent wall thickness for precise thermal transfer
- Automation friendly, single notch corner, stackable
- Made from 99.9% virgin polypropylene
- 240ul well capacity (working capacity dependent on closure method)
- Barcoding available
- Certified DNAse/RNAse and pyrogen-safe