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ActionsPCR/qPCR
MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more. If you are looking for any of these things, we can match almost all needs to all cyclers. If you don’t find it easily contact us at tech@midsci.com and we will find it for you.
96 or 384 we have it. Non-Skirted, semi-skirted or full skirted, we have it. 0.1, 0.2, or 0.5 mL we have it. A1, A12, H12, A24, P24 cuts, we have them. Sybr© Green or probe alternative, we have it.
Pro Tip: Colored plastics do not have any impact on DNA amplification. But when setting up qPCR it is often recommended to use white plastics. This will limit or prevent fluorescence refraction. Light refraction can minimize sensitivity and consistency.
Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips. This will help optimize and reduce variance in the reactions. Simple test, see the residual liquid left in tips? This means some of the taq, dNTPs, sample, etc is left in the tip. PR1MA tips help reduce that significantly and improve your consistency.
Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate? Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler. Call us we have solutions.
Accuris qMAX One-Step RT-qPCR kits
- RNA to cDNA to qPCR, in one tube
- High purity enzyme formulation for enhanced stability and performance
- Accuris Hot-Start Taq allows for preparation at room-temperature
- Blue dye facilitates pipetting and visualization in plates
- Available for green fluorescence or probe detection
- Multiplex formulation available for multiple target amplification
- Bulk pricing available for high throughput labs and kit manufacturers contact us for details
Accuris qMAX™ One Step Kits allow for highly sensitive real time RT-qPCR assays to be performed directly from RNA templates. Workflows are simplified with optimized formulations of ready-to-use 2X qPCR master mix and 20X reverse transcriptase.
Optimized buffer includes powerful RNase inhibitors, and an extremely thermostable MMLV-derived reverse transcriptase enables robust first strand cDNA synthesis. Accuris Hot Start Taq uses an antibody mediated hot start mechanism allowing for sample preparation at room temperature. Only after an initial incubation at 95C will the Taq become active, so non-specific amplification is greatly reduced. An inert blue dye is included in the Taq master mix to help simplify pipetting and reduce errors.
Three versions of our One Step qPCR kits are available:
qMAX Green One Step kits incorporate our proprietary intercalating dye which exhibits higher fluorescent and lower PCR inhibition than other popular dyes such as SYBR.
qMAX Probe One Step kits are optimized for use with popular TaqMan, Scorpions, and molecular beacon probes.
qMAX Probe One Step Multiplex kits are specifically developed and optimized for efficient probe-based detection of multiple targets in a single reaction well. The Multiplex formulation is comprised of a 20x reverse transcriptase and 2x PCR Mix preparation ideally suited for complex RNA samples including low-copy number viral RNA commonly used in the clinical and research laboratory. qMAX Probe One Step Multiplex kits have been designed to overcome the many challenges of multiplex RT-PCR, by addressing important factors such as the balance between magnesium chloride and deoxynucleotide concentrations, the relative Taq Polymerase and reverse transcriptase concentration, and the ionic conditions of the core reaction buffer.
All Accuris One-Step Kits are compatible with standard and fast cycling protocols and provide increased sensitivity, speed, and reproducibility for a broad range of samples and targets. The polymerase mix is available with different levels of ROX reference dye for compatibility with all qPCR instruments.
PurePlus® PCR Plates
- PurePlus® plates are tested for RNase/DNase lot by lot to ensure pure product
- Our plates are lot certified free of Human DNA contamination
- Our plates are tested for ATP lot by lot to ensure pure consistent product
- Broad product range. Over 18 styles of plates fit most major thermocyclers
- These plates are autoclavable to 122° C for 15 minutes at 15PSI
Ultra Clear and Ultra Clean
We make our PurePlus® Polymerase Chain Reaction (PCR) consumables without additives so you don't need to wonder what is different about the plates, tubes, or strips you are using this week. We also test every batch for contamination by Human DNA as well as the more common tests that other suppliers offer for RNase and DNase enzymes. Many of our plates are printed with black alpha numeric reference grids to make it easier for you to keep track of your samples. We also offer optically clear caps and opaque black or white plates for Real Time PCR. And we also offer re-usable silicone sealing mats and polypropylene sealing film. Labcon PurePlus® PCR tubes, strips and plates are designed to fit many popular thermocyclers including brands like Applied Biosystems, Eppendorf, Thermo Scientific, and Bio Rad. All our PCR disposables are made from 100% virgin polypropylene and are lot tested and certified to be free of RNase, DNase, and Human DNA. Autoclavable. Our pure resin consumables are available in strips, individual tubes, plates in clear natural or colors.
Our PurePlus® sterile disposables, like all our other sterile products, receive valid sterilization processing compliant with ISO 11137. So unlike "pre-sterile" products from other suppliers you can use PurePlus® PCR disposables for diagnostic and regulated procedures.
MIDSCI # | VWR # |
3983-520-000-9 | 82006-678 |
3983-520-010-9 | 89218-288 |
3984-520-000-9 | 89218-294 |
3961-520-000-9 | 89218-296 |
3962-520-000-9 | 89218-298 |
3962-521-000-9 | 10755-256 |
3971-520-000-9 | 82006-650 |
3976-520-000-9 | 82006-664 |
3978-520-000-9 | 82006-704 |
3978-520-010-9 | 89218-292 |
3978-522-000-9 | 82006-714 |
3978-524-000-9 | 82006-710 |
3978-526-000-9 | 82006-712 |
3978-527-000-9 | 82006-706 |
3978-528-000-9 | 82006-708 |
3978-529-000-9 | 82006-718 |
3963-520-000-9 | 83009-676 |
3967-520-000-9 | 83009-668 |
3968-520-000-9 | 83009-672 |
3972-520-000-9 | 89049-178 |
3972-520-010-9 | 89218-290 |
3973-520-000-9 | 83007-374 |
3973-529-000-9 | 83007-372 |
3974-520-000-9 | 37001-272 |
3977-520-000-9 | 82006-636 |
3977-522-000-9 | 82006-646 |
3977-526-000-9 | 82006-644 |
3977-527-000-9 | 82006-638 |
3977-528-000-9 | 82006-640 |
3977-529-000-9 | 82006-648 |
3979-520-000-9 | 93001-100 |
- Plate Characteristics
- 96 x 0.1mL, Sub-skirted
- Low Profile (0.1mL), Frosted (low signal to noise ratio
- Extreme Uniformity (EU) Plate Design
- Uniform wall thickness ensures consistent even heating
- Thin wall-bottomed tube for rapid heat transfer
- Ideal paring with EU Caps with "Sealing Ring Band" Design
- Easy open - easy close with ergonomic caps: B57801, B79701, C79701
- Caps are significantly easier to apply and remove -- Repetitive Strain Injury (RSI) Friendly.
- Significant reduction in evaporation due to a wider sealing area.
- Certified DNA, DNase, RNA, RNase and pyrogen-free
Please give us a call for a sample!
Order# |
Description |
Non-Skirt |
|
B70501 |
Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, Natural, 25/cs |
B70509 |
Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, White, 25/cs |
B50501 |
Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, Frosted, 25/cs |
AB70651 |
Pryme PCR Plate, 96-Well, 0.1mL, Non-Skirt, Low Profile, Frosted, 25/cs |
Semi-Skirted |
|
B70651 |
Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, Natural, 25/cs |
B70659 |
Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, White, 25/cs |
B50651 |
Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, Frosted, 25/cs |
B17480 |
Pryme PCR Plate, 96-Well, 0.1mL, Semi-skirted, Low Profile, Frosted, 25/cs |
B17489 |
Pryme PCR Plate, 96-Well, 0.1mL, Semi-skirted, Low Profile, White, 25/cs |
Sub-Skirted |
|
ABI7501 |
Pryme PCR Plate, 96-Well, 0.2mL, Sub-skirted, Regular Profile, Frosted, 25/cs |
ABI7509 |
Pryme PCR Plate, 96-Well, 0.2mL, Sub-skirted, Regular Profile, White, 25/cs |
AB19800-EU |
Pryme PCR Plate, 96-Well, 0.1mL, Sub-skirted, Low Profile, Frosted, 25/cs |
AB19809-EU |
Pryme PCR Plate, 96-Well, 0.1mL, Sub-skirted, Low Profile, White, 25/cs |
Caps |
|
B57801 |
Pryme PCR Ergonomic 8-Strip, Optical Flat (V2) Caps, Natural, 120/cs |
B79701 |
Pryme PCR Ergonomic 8-Strip, Optical Flat (V1) Caps, Natural, 120/cs |
C79701 |
Pryme PCR Ergonomic 8-Strip, Semi-Domed (V1) Caps, Natural, 120/cs |
Plate-Sealing Roller
Application: Plate-sealingMaterial: Rubber, metal
The plate-sealing roller features a smooth spinning, thermoplastic elastomer sealing surface with a durable plated metal handle.
- Reusable, autoclavable
- Provides a large 4 inch (10.16 cm) wide sealing surface
- A 10 minute or greater dwell me at room temperature before processing increases the film-to-plate bond
We suggest purchasing PR1MA PCR Plastics.
Contact your rep or email tech@midsci.com for more information.
- Standard thermal cycling and qPCR compatible
- Ultra-thin and consistent wall thickness for precise thermal transfer
- Automation friendly, single notch corner, stackable
- Made from 99.9% virgin polypropylene
- 240ul well capacity (working capacity dependent on closure method)
- Barcoding available
- Certified DNAse/RNAse and pyrogen-safe
- Standard thermal cycling and qPCR compatible
- Ultra-thin and consistent wall thickness for precise thermal transfer
- Single notch corner
- Made from 99.9% virgin polypropylene
- 340ul well capacity (working capacity dependent on closure method)
- Fits standard thermal cyclers, plus ABI PCR 2700, 2720, 9700, 9800, 7300, 7500, 7900, and ABI sequencing units
- Certified DNAse/RNAse and pyrogen-safe