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PCR/qPCR
MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more. If you are looking for any of these things, we can match almost all needs to all cyclers. If you don’t find it easily contact us at tech@midsci.com and we will find it for you.
96 or 384 we have it. Non-Skirted, semi-skirted or full skirted, we have it. 0.1, 0.2, or 0.5 mL we have it. A1, A12, H12, A24, P24 cuts, we have them. Sybr© Green or probe alternative, we have it.
Pro Tip: Colored plastics do not have any impact on DNA amplification. But when setting up qPCR it is often recommended to use white plastics. This will limit or prevent fluorescence refraction. Light refraction can minimize sensitivity and consistency.
Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips. This will help optimize and reduce variance in the reactions. Simple test, see the residual liquid left in tips? This means some of the taq, dNTPs, sample, etc is left in the tip. PR1MA tips help reduce that significantly and improve your consistency.
Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate? Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler. Call us we have solutions.
Hoefer® Mighty Small II Mini Vertical Electrophoresis System
Designed for quick screening and rapid separation.
- Maximum glass plate size 10 x 8 cm
- Efficient active cooling ensures sharp bands
- Quick and easy to assemble
- Requires minimal buffer
Easily upgradable to the SE260 (with optional accessories), resulting in the additional capability of accommodating both 10 x 8 and 10 x 10.5 cm gels, enabling a wider variety of precast gel options.
Hoefer® Mighty Small II Complete Units (SE250-10A)
- Lower Buffer Chamber, 1/ea
- Upper Buffer Chamber with Cooling Core, 1/ea
- Safety Lid with High Voltage Leads, 1/ea
- Casting Cradle with Sealing Gasket Set, 1/ea
- Casting Clamp Assemblies, 2/ea
- Cams, 4/ea
- Glass Plates, Rectangular, 10 x 8 cm, 10/ea
- Alumina Plates, Notched, 10 x 8 cm, 2/ea
- Spring Clamps, 4/ea
- Combs, 10 well, 2/ea
- T-Spacers, 4/ea
- Well Locating Decals, 2/ea
- Gel Seal, 1/ea
Hoefer® Mighty Small II Basic Unit (SE250)
The same as above without the combs, spacers, and caster. For use with precast gels or order two SE211A combs, two sets of 8 cm long T-spacers, 10 x 8 cm rectangular glass and notched alumina plates, and a gel caster.Specifications
| Plate Size (W x H) | 3.34 x 3.15 In. | 10 x 8 cm |
| Spacers | 0.75 mm, 1.0 mm, 1.5 mm |
| Max. Power | 500 V, 500 mA, 12 W |
| Max. Temp. | 113ºF | 45°C |
| Indoor Use | 39.2 to 104ºC | 4 to 40°C |
| Humidity | Up to 80% |
| Dims (D x W x H) | 6.30 x 6.50 x 6.30 In. | 16 x 16.5 x 16 cm |
| Weight | 3.30 lb | 1.50 kg |
Proudly made in the USA! Click here for more made in America products!
Hoefer® Air-Cooled Vertical Electrophoresis System
Compact, rugged, and economical units ideal for educational laboratories with multiple users.
- Maximum glass plate size 18 x 16 cm
- Rugged injection molded construction is durable and assembles easily
- Run two gels with the addition of a divider plate
- Built-in casting stand seals leak free
- Choose from a wide variety of accessories
- Plates, clamps, spacers, and combs are interchangeable with the SE600 Series
Hoefer® Air-Cooled Complete Unit (SE400-15-15)
- Lower Buffer Chamber/Casting Stand on Leveling Base with High Voltage Leads, 1/ea
- Upper Buffer Chamber, 1/ea
- Safety Lid with Electrodes, 1/ea
- Glass Plates, 18 x 16 cm, 2/pk
- Clamp Assemblies, 16 cm, 2/pk
- Cams, 2/pk
- Slotted Gasket for Upper Buffer Chamber, 1/ea
- Bubble Level, 1/ea
- Laminated Gasket for Casting Stand, 1/ea
- Spacer-Mate-Alignment Template, 1/ea
- Wonder Wedge Plate Separation Tool, 1/ea
- Gel Seal, 1/ea
- Comb, 15-well, 1.5 mm thick, 1/ea
- Spacers, 1.5 mm thick, 2 cm wide, 2/pk
Hoefer® Air-Cooled Basic Unit (SE400)
The same as above without the combs and spacers. Order one SE511 comb and one set of 16 cm long spacers separately.
Specifications
| Plate Size (W x H) | 7.09 x 6.30 In. | 18 x 16 cm |
| Spacers | 0.75 mm, 1.0 mm, 1.5 mm |
| Max. Power | 500 V, 60 mA, 20 W |
| Max. Temp. | 113ºF | 45°C |
| Indoor Use | 39.2 to 104ºF | 4 to 40°C |
| Humidity | Up to 80% |
| Dims (D x W x H) | 5.91 x 9.45 x 11.02 In. | 15 x 24 x 28 cm |
| Weight | 6.83 | 3.10 kg |
Proudly made in the USA! Click here for more made in America products!
Hoefer® Max Horizontal Agarose Electrophoresis System
Offers a variety of gel lengths and comb sizes to enable the design of the gel required for optimum resolution of DNA fragments. Facilitates high-resolution separation of nucleic acid fragments 100 bp to 20 kbp. Three different length trays are available for use with this unit.
- Self-locating/centering guides support consistent running tray placement
- Gel anchor feet prevent the gel from floating or sliding during the run
- A variety of comb sizes enables customization of sample throughput to fit your laboratory's requirements
- Adjustable comb height—complete control over well depth
- Three casting kits available—enabling users to run 15 x 20, 15 x 15, or 15 x 10 cm gels in the same unit
- Gel Casting Kit Includes 15 x 10, 15 x 15, or 15 x 20 cm Running Tray, 15 x 10, 15 x 15, or 15 x 20 cm Casting Tray, and 4 Foam Gaskets
Complete Unit (HE99X-15-15)
- Buffer Chamber Assembly, 1/ea
- Safety Lid with High Voltage Leads, 1/ea
- Running Tray, 15 x 20 cm, 1/ea
- Casting Tray, 15 x 20 cm, 1/ea
- Foam Gaskets, 4/pk
- Bubble Level, 1/ea
- 1.5 mm thick, 15-well comb, 1/ea
- Comb Back, 1/ea
Basic Unit (HE99X)
The same as above, without the Running Tray, Casting Tray, Comb, and Comb Back. Order a casting kit, combs, and comb backs separately.
Specifications
| Gel Dims (W x L) | 5.91 x 7.87 | 5.91 x 5.91 | 5.91 x 3.94 In. 15 x 20 | 15 x 15 | 15 x 10 cm |
| Max. Buffer Vol. | 1.2 L |
| Max. Power | 200 V, 100 mA, 20 W |
| Max. Capacity | 60 Samples |
| Max. Temp. | 113ºF | 45°C |
| Indoor Use | 39.2 to 104ºF | 4 to 40°C |
| Humidity | Up to 80% |
| Dims (D x W x H) | 14.17 x 7.17 x 5.51 In. | 36 x 18.2 x 14 cm |
Proudly made in the USA! Click here for more made in America products!
MyView™ Compact UV Transilluminator
- Uniform Illumination | Four 302 nm Bulbs
- Adjustable UV Safety Cover | Easy Access to Gels
- Large Scratch Resistant Viewing | 16.5 x 13.5 cm
The E3100 Compact UV Transilluminator has a surprisingly small footprint (just over 12 x 8 In.) yet provides industry leading performance. It will accommodate one or two mini gels on the 16.5 x 13.5 cm scratch resistant viewing surface.
The integrated UV blocking cover is made from a polymer that will not fluoresce in UV light. This allows clear viewing of DNA bands through the cover yet offers full UV blocking protection. Two friction hinges on the front edge of the cover allow for full adjustment. Position the cover horizontally for general viewing, tilt it at an angle for gel access and band cutting, or open fully to use the SmartDoc™ Imaging Enclosure (sold separately) for gel imaging with a smartphone.
The transilluminator’s four 6W bulbs evenly illuminate the viewing surface, and the 302 nm wavelength is ideal for Ethidium Bromide, SmartGlow™, SYBR Green®, Gel Green™ and many other common gel stains. Spacing of the UV bulbs, coupled with a reflector system and special diffusing filter glass provides exceptional uniformity across the viewing surface. This allows for quantification of DNA and higher quality imaging when used with a camera system.
Specifications
| Light source 4 x 6W UV Light Bulbs | 4 x 6W UV Light Bulbs |
| Safety cover UV blocking polycarbonate | UV blocking polycarbonate |
| Exterior dimensions | 10 x 8 x 2.75 In. | 25.4 x 20.32 x 6.99 cm |
| Electrical | 12VDC (AC power adapter included) |
| Output wavelength | Peak at 302 nm |
| Viewing surface | 6.5 x 5.3 In. | 16.5 x 13.5 cm |
| Weight | 2 lbs. | 0.9 kg. |
| Warranty | 2 years |
Item # E3100 is proudly made in the USA! Click here for more made in America products!
Hoefer® Power Supplies
Small footprint, large handle, and simple operation make Hoefer® Power Supplies easy to set up and use in your lab. With a large, easy-to-read LED, simply set the constant parameter, set the timer (if desired), and push start.
- Constant voltage or constant current mode with automatic crossover
- Four sets of 4 mm output jacks
- Continuous runs or a 999-minute timer
- Automatic recovery after power failure
- Open circuit detection
- Short circuit protection
Modes of operation
Constant Voltage Mode: the power supply will attempt to maintain the user selected voltage by automatically adjusting the current flow.
Constant Current Mode: the power supply will attempt to maintain the user selected current flow by automatically adjusting the voltage supplied.
Automatic Crossover: when the constant parameter (voltage or current) cannot be maintained due to power supply operating limits, the power supply automatically switches to maintaining the other parameter.
- PS300B 300 Volt Power Supply is designed for most electrophoresis techniques.
- PS200HC 200 Volt High Current Power Supply is ideal for large electrophoretic blotting applications and for low voltage electrophoresis runs.
- PS600 600 Volt Power Supply is designed for electrophoresis and blotting techniques, including large format and high throughput applications.
Hoefer® Mini Electrophoresis and Western Blot Package
Save time and money with a combination package.
- SE260-10A-75 Mighty Small II Mini Deluxe Vertical Unit
- TE22 Mighty Small Mini Transfer Tank
- PS300B 300 V, 500 mA, 90 W Power Supply
Specifications
| Item # | PS300B | PS200HC | PS600 |
| Voltage | 10 to 300 V in 1 V step | 5 to 200 V in 1 V step | 10 to 600 V in 1 V step |
| Current | 4 to 500 mA in 1 mA steps | 10 to 2000 mA in 1 mA steps | 4 to 750 mA in 1 mA steps |
| Max. Wattage | 90 W | 200 W | 150 W |
| Timer | 0 to 999 minutes | 0 to 999 minutes | 1 to 999 minutes, 1 min increment |
| Output Modes | Constant voltage or current | ||
| Output Jacks | 4 pairs in parallel, 4 mm plugs | ||
| Indoor Use | 39.2 to 104ºF | 4 to 40°C | ||
| Humidity | 80% | 80% | 0 to 80%, noncondensing |
| Voltage Input | 100 to 240 VAC, 50/60 Hz | ||
| Dims (D x W x H) | 11.02 x 4.92 x 13.18 In. | 28 x 12.5 x 33.5 cm | ||
| Weight | 5.29 lb | 2.4 kg | ||
Proudly made in the USA! Click here for more made in America products!
Labnet™ 1001 Mini Plate Spinner Centrifuge
Compact Microplate Centrifuge for PCR and Microplate Samples.
The Labnet™ 1001 Mini Plate Spinner is the first centrifuge specifically engineered for rapid, efficient centrifugation of PCR plates and standard microplates. Designed with the modern lab in mind, this compact microplate centrifuge takes up minimal bench space with a footprint of just 6 x 6 In., making it ideal for crowded workspaces and portable setups.
Unlike bulky traditional centrifuges, the MPS 1001 features a unique vertical loading slot that allows you to insert two sealed microplates directly from the top. Surface tension holds the samples in place during spinning, preventing spills or cross-contamination.
With a quick acceleration to 2,500 rpm, this microplate spinner ensures your samples are ready in seconds. Simply close the lid to start the spin cycle and press the open button to engage the brake for immediate rotor stop.
Ideal for temperature-sensitive procedures, the MPS 1001 is fully operational in cold room environments, making it a versatile and essential tool for any PCR workflow or high-throughput screening process.
Key Features
- Designed specifically for PCR plates and microplates
- Ultra-compact footprint: 6 x 6 In.
- Holds two standard microplates vertically
- Spins at 2,500 rpm for quick sample settling
- Operates in cold rooms for temperature-sensitive samples
- Easy one-touch operation with fast braking
MPS Adapter Supports
- 8 x 0.2 mL PCR Strips
- 12 x 0.2 mL PCR Strips
- 0.2 mL PCR Tubes
- 48 Well PCR Plates
Specifications
| Capacity | 2 x 96 / 384 Well PCR Plates |
| Dimensions | 7.5 x 8.6 x 7.3 In. | 190 x 220 x 186 mm |
| Weight | 5.7 lbs | 2.6 kg |
| Power Supply | 100 to 240V~, 50/60 Hz |
| Max. Speed | 2,500 rpm ±10% |
| Max. RCF | 500 x g |
| Temp. Range | 5 to 40°C (up to 80% RH at 31°C, decreasing linearly to 50% RH at 40°C) |
Accuris SmartBlue™ Transilluminators
Accuris SmartBlue™ Transilluminators have been developed for viewing DNA gels stained with new, safe fluorescent dyes. These new dyes, such as SmartGlow™, SYBR Green, Gel Green, have an excitation wavelength of 465-470 nm (visible blue light), and are safer to use than Ethidium Bromide.
- Blue light, safe for nucleic acids
- Uniform illumination (UI Technology)
- Optimal wavelength (465nm) for common safe stains
- Adjustable position, amber filter covers
- Optional amber viewing glasses: E4000-VG1
Safer for samples, safer for you: Accuris SmartBlue™ Transilluminators are the perfect partner for the most common safe DNA stains. These instruments emit blue light at a wavelength of 465 nm, perfect for excitation of most green fluorophors, including our Accuris’ SmartGlow™, SYBR Green, SYBR Safe, SYBR Gold, GelStar, GelGreen, to name a few. SmartBlue™ transilluminators can also work for excitation of EtBr (though fluorescence is not as bright as with UV), see our Technical Bulletin with sample images. Exposing DNA to blue wavelength does not cause molecular damage, and results in a much higher downstream cloning efficiency as compared to even short UV light exposures. In addition, the visible blue light is not damaging to the user’s skin and is less harmful to the eyes.*
Adjustable filter cover: Both the E4101 and SB4500 transilluminators come with emission filter covers. These amber covers, which rest above the viewing surface and gel, filter out the blue light, allowing the fluorescing DNA bands to be seen clearly. The covers can be set to a flat position for general gel viewing or can be set at an angle to provide easy access to the gel for band excision. The SB4500 has a two-position filter cover, and the E4101 features a hinged filter cover.
Optional amber viewing glasses: Our E4000-VG1 SmartBlue™ Plus Viewing Glasses offer efficient blocking of the blue light wavelength from the transilluminators, allowing clear visualization of the fluorescing DNA bands without the filter cover in place. These convenient glasses are recommended for users that are frequently excising DNA bands from gels. SmartBlue™ Plus glasses also block 100% of UV wavelength so they can also be used for viewing gels on a UV transilluminator.
UI technology, bright and clear images: The bright LED light plates and filter layer provides an extremely evenly lit viewing surface. Even in ambient light, bands of DNA can clearly be seen in stained gels. Images obtained using the SmartBlue™ Transilluminator are brighter and more balanced than those obtained using epi-illuminators which light from the sides.
Reliable, durable, and long-lasting: An array of super bright LEDs with a long, 30,000 hour service life provide the light source for the SmartBlue™ Transilluminators. Unlike those in a UV transilluminator, the filters in the SmartBlue™ systems will not solarize and degrade in performance over time. Gel bands can be excised directly on the scratch resistant, glass viewing surfaces. To save energy, the power switch includes an automatic 5 minute shutoff. SmartBlue™ Transilluminators are covered by a 2 year warranty.
* SmartBlue™ products use high intensity LED lights. Although the visible blue wavelength is significantly safer than UV light, it is recommended to not stare directly at the viewing surface without the amber cover in place.
Specifications
| Light Source | High Intensity Blue LEDs |
| Emission Filter Cover | Amber |
| Wavelength | Peak at 465 nm |
| E4000 Viewing Surface | 17 x 12 cm |
| E4000 Ext. Dimensions | 30.5 x 21.5 x 5 cm |
| E4101 Viewing Surface | 10.5 x 10.5 cm |
| E4101 Ext. Dimensions | 17 x 12 x 5 cm |
| Electrical | 100 to 240V, 50-60Hz |
| E4000 Weight | 1.2kg / 2.6lbs |
| E4101 Weight | 0.34kg / 0.75lbs |
| Warranty | 2 years |
Item # SB4500 is proudly made in the USA! Click here for more made in America products!
Adjustable Well Plate Stand
Achieves accurate pipetting by allowing the flexibility to adjust the angle of the microplate (45° max. tilt). The stand takes up little benchtop space measuring only 6 x 4.5 x 2.5 In. (D x W x H).
*Well Orienters are sold separately.Proudly made in the USA! Click here for more made in America products!
LabValues 2025 Promo!
For a limited time, enjoy special pricing on select Labnet Enduro™ Power Supplies!
(E0303, E0203)
Offer cannot be combined with other discounts or institutional pricing.
Offer not valid on previous purchases. Promo valid from 7/15/25 to 12/31/25 or while supplies last.
All terms and conditions apply.
ENDURO™ Power Supply
- Two models (300V and 250V) available
- For DNA, RNA, or protein electrophoresis, and blotting applications
- Constant voltage or constant current with automatic crossover
- 250 V model also offers a constant power operating mode
- Timed or continuous operation
- Short circuit and over current/ over voltage protection
- No load and load change detection system
- Programming includes option for automatic recovery after power failure
- 3 year warranty
The new Enduro™ Power Supplies meet a wide range of applications including DNA, RNA, and protein electrophoresis, blotting, and many others. Two models are available with voltage ranges to 300 V and current ranges to 3000 mA. Operating modes include constant voltage or constant current with automatic crossover. In addition, the 250V model offers a constant power operating mode.
All parameters, including voltage, current, power (250 V model only), and time are easily set using the membrane keypad and displayed digitally on the back lit LCD. Compact in design with a small footprint, the Enduro™ Power Supplies are also stackable to save space. For easy viewing and setting of parameters on the bench or on a shelf, the front of the power supply can be raised with the integral folding feet.
Specifications
| Model | 300V | 250V |
| Output Voltage Range/Increments | 2-300V/1V | 5-250V/1V |
| Output Current Range/Increments | 4-500mA/1mA | 10-3000mA/10mA |
| Output Power Range/Increments | 90 watts max/n.a. | 1-300W/1W |
| Operating Constant Modes | current or voltage | current, voltage, or power |
| Timer | 1 -99 min, or continuous | 1min. to 99h 59min or continuous |
| Programmable | no | Yes (20 program capacity) |
| Dimensions (W x D x H) | 19 x 25 x 8 cm | 19 x 25 x 8 cm |
| Weight | 2.6 lbs | 5.7 lbs |
| Input Voltage | 110-230 V | 110-230 V |
Multi-colored 96 Well Orienter
For easy identification of samples in microplates, vertically or horizontally oriented bright colors make it easy to distinguish between columns. Fits most standard flat and round bottom 96-well plates. Available in horizontal and vertical orientations.
Proudly made in the USA! Click here for more made in America products!
LabValues 2025 Promo!
For a limited time, enjoy special pricing on Labnet Enduro™ UV Transilluminators!
Offer cannot be combined with other discounts or institutional pricing.
Offer not valid on previous purchases. Promo valid from 7/15/25 to 12/31/25 or while supplies last.
All terms and conditions apply.
- Compact size with 21 x 26 cm viewing area
- Available in single or dual wavelength models
- Unique heavy duty hinge design can be held in place at any angle for gel cutting
- UV shield fully protects user from UV light
- Uniform UV Black Glass provides improved imaging contrast
This brand is being discontinued and will only be available while supplies last.
Need a great DNA Safe Stain at a great price?
Try PR1MA! Click here to order.
Bullseye DNA SafeStain,10,000x
- Safest DNA stain by far
- Replaces EthBr in agarose gel electrophoresis
- Detection of DNA and RNA
- Excitation at 290 nm & 490 nm
- Emission at 530 nm
DNA SafeStain is a new and safe nucleic acid stain for visualization of double-stranded DNA, single-stranded DNA, and RNA in agarose gels. The dyes are developed to replace toxic ethidium bromide (EthBr, a potent mutagen), commonly used in gel electrophoresis for visualization of nucleic acids in agarose gels. DNA SafeStain is non-carcinogenic by the Ames-test. The results are negative in both the mouse marrow chromophilous erthrocyte micronucleus and mouse spermary spermatocyte chromosomal aberration tests.
DNA SafeStain emits green fluorescence when bound to DNA. It has two excitation wavelength peaks when bound to nucleic acid, at 290nm and 490nm and the emission can be detected at 530nm.
Storage:
Store under dark at 4°C or room temperature.
User Instruction:
DNA SafeStain can be used in the exact same way as EtBr in agarose gel electrophoresis. For example, add 100µl to 1 liter of 1X running buffer (1xTAE or 1x TBE), and use this DNA SafeStain containing buffer to make the agarose gel and run the gel.
Special Notes:
1. For best results, dilute the DNA SafeStain 1:10,000 in your gel running buffer and use this dye-containing running buffer to make your agarose gel and to run the gel.
2. As is the case with EtBr; add more DNA SafeStain, if brighter DNA bands are desired. To view the results, any conventional DNA gel viewing light box or gel documentation system should work well with the DNA SafeStain; although, using a LED light instead of a UV light source is preferred due to the harmful nature of UV light.
This Product is For Research Use Only
*For the proper disposal of this product, follow University or Company Guidelines.