Home  >  PCR, Protein, RNA, DNA  >  PCR

PCR/qPCR

MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more.  If you are looking for any of these things, we can match almost all needs to all cyclers.  If you don’t find it easily contact us at tech@midsci.com and we will find it for you. 

96 or 384 we have it.  Non-Skirted, semi-skirted or full skirted, we have it.  0.1, 0.2, or 0.5 mL we have it.  A1, A12, H12, A24, P24 cuts, we have them.  Sybr© Green or probe alternative, we have it.  

Pro Tip: Colored plastics do not have any impact on DNA amplification.  But when setting up qPCR it is often recommended to use white plastics.  This will limit or prevent fluorescence refraction.  Light refraction can minimize sensitivity and consistency. 

Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips.  This will help optimize and reduce variance in the reactions.  Simple test, see the residual liquid left in tips?  This means some of the taq, dNTPs, sample, etc is left in the tip.  PR1MA tips help reduce that significantly and improve your consistency. 

Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate?  Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler.  Call us we have solutions.

|◀ ◀ 265 - 276 of 293 ▶ ▶|
View:

ENDURO Modular Vertical Gel System


A modular system allowing PAGE, electroblotting and 2D gel electrophoresis to be carried out in the same tank, simply by changing the inserts. Users can purchase only the parts they need, or a complete combined system for a cost savings over buying all three systems separately.

  • Modular Design
  • PAGE, Electroblotting and 2D Electrophoresis
  • Quick and easy set up
  • Wide range of accessories
 
MODULAR SYSTEM 
 
The modularity of the ENDURO™ System is based on three operational inserts and a common buffer tank. All three inserts, PAGE, electroblotting and 2D electrophoresis, have their own electrode assembly that connects into the lid of the buffer tank. PAGE INSERT Set up of gels is quick and easy with the PAGE insert. A set of plates are placed on either side of the insert and held into place by the pressure bar. An additional gel may be run on each side by stacking the plates. The assembly is then placed into the casting stand for pouring the gel. After the gel is polymerized, the insert is removed from the casting stand and placed in the buffer tank.
 

ELECTROBLOTTING INSERT
 
The electroblotting insert has a capacity for 3 gels. Setting up blots is easy - gels are sandwiched in the blotting cassettes with the transfer membranes. The cassettes are then placed in the insert, which is in turn placed in the buffer tank. Coiled electrodes in the insert generate a high intensity current, allowing transfer to take place in about an hour.
 

2D ELECTROPHORESIS INSERT
 
This insert is used for running the first dimension of IEF gels. Up to ten 8 cm gels are poured in disposable capillary tubes and placed in this insert. Gasketed ports allow for easy insertion and removal of tubes. Blocking plugs are included for closing off ports that are not in use.
 

BUILT FOR SAFETY AND ENDURANCE
 
For leak-proof performance and a long service life, the buffer tank is molded from high quality acrylic. Pins on the top of the inserts ensure that the lid is placed onto the insert in the proper orientation. These pins also aid in removal of the lid. Removing the lid disconnects power to the electrodes.

 
ACCEPTS MOST PRE-CAST GELS
 
Most precast gels, including NuSep™, Bio-Rad™, Invitrogen™, and Cambrex™, can be used with the ENDURO™ PAGE System. The use of precast gels saves time by eliminating the need to pour gels and wait while they polymerize.

 
WIDE RANGE OF ACCESSORIES
 
A variety of combs and spacers of varying widths are available to customize your ENDURO™ system. All are color coded and clearly marked for easy identification. Both notched and plain plates can be purchased with spacers permanently bound for easy set up. Additional fiber pads and replacement cassettes are also available as well as capillary tubes, blanking ports and tube supports. Cooling packs are also available to help reduce buffer temperature during higher voltage or longer runs.
Item#:
ASEDIROMOVERGES

Minigel System w/ 4 comb slots, 12 cm x 14 cm

Horizontal gel electrophoresis system for 12cm x 14cm minigels. Includes gasketed UVT gel tray with fluorescent ruler, and (1) 12 and (1) 20 tooth comb, 1.5 mm thickness. Comb substitutions are available. Four year manufacturer warranty.
 
System Description

This flexible system from Galileo is the perfect personal unit for a wide range of separation needs, and our most popular model.

Unit features RapidCast trays for quick in-unit gel casting of 12cm wide by 14cm long gels.

Choose a complete system or build your own custom unit from the many system components we offer.  The NEBT-80-1214 gel system is supplied with a RapidCast™ gasketed gel tray for convenient gel casting directly in the buffer chamber.  Choose from a wide variety of comb options.  All are compatible with Owl Separation Systems B2A or similar devices.  The teeth on combs marked as "microtiter" are uniformly spaced for easy loading with multichannel pipettors.System features

System Features

The Galileo 1214 complete system includes these features:

  •     Heavy duty lower buffer chamber
  •     Interlock safety lid with attached leads
  •     Gasketed (cast in place) 12cm wide x 14cm long UV-transparent gel tray with
  •     built-in fluorescent ruler and 2 comb slots.
  •     One 1.5mm x 12 tooth comb
  •     One 1.5mm x 20 tooth comb

System Options
 
The Galileo 1214 unit can be also be ordered with our new automatic buffer recirculation system (80-1214-R) or with installed buffer ports for connection to an external buffer recirculation system (80-1214-BEP)
Item#:
ASMINGEL412AND14

Electrophoresis System Bundle


Package Includes:

  • Complete PR1MA™ myGel™ Mini system (E1101; includes gel box, power supply, and casting sets)
  • SmartDoc™ 2.0 Gel Viewing and Smart Phone Imaging System (E5001-SDB)
  • SmartGlow DNA Stain, 1.0 mL (BE4500-PS or BE4500-LD)
  • Agarose LE, 100 grams
Item#:
ASESYSBUNDLE

Horizontal Gel System, 15cmW x 25cmL


Galileo large gel systems are designed for high resolution of complex analytes. All units feature long run lengths, with medium to high capacity units available. High quality 9mm thick rugged acrylic construction, resists cracking and warping.
 
Your safety ensured with interlocking lid, protected sockets and permanently attached power cords. Epoxy sealed electrode connections, resist corrosion and leaks. Gold plated banana plugs.
Meets or exceeds IEC1010-1 standards. Safe & Reliable UV transmissible gel trays with built-in fluorescent rulers.

Large units feature trays with multiple comb slots for veratility. Machined finger grooves on the tray sides for easy and sure handling. Wide range of comb configurations, including multi-channel capability. Selected units available with buffer recirculation capabilities. Combs specifications clearly marked on each comb. Optional casting methods for flexibility, convenience and speed.

Convenient & Versatile
 
  • Exceptional resolution.
  • Platinum electrodes configured to provide uniform field for straight runs.
  • Multiple comb slot options for a wide range of sample and run-length configurations.
Item#:
ASHORIZGELSY15
  • This unique, new design for the Life Science Laboratory allows crosslinking 
    and hybridization at one location
  • Hybridizer and Crosslinker portions of the HL-2000 operate independently of each other
  • The Crosslinker is also available as a stand-alone unit
  • Temperature control (ambient to 99.9°C)
  • Variable speed control (10 to 18 RPM) enabling consistent saturation of samples, whether it be for washing or hybridizing
  • Large LED display
  • Stainless steel internal construction
  • Multiple bottle sizes, arrangements and off-set bottle positions (three bottle sizes available)
  • Optional rocker plate
    In addition to the hybridization oven features mentioned above, the Crosslinker features:
  • Preset and manual controls for ultraviolet or time exposures
  • Preset exposure delivers 120,000 microjoules or five minutes of exposure
  • Shortwave 254nm, 8 watt, ultraviolet tubes
  • Microprocessor measures and controls UV output, ensuring maximum energy efficiency
  • Large LED display continuously displays time or energy settings
  • Tactile membrane switch keypad
  • Window on the door allows viewing of the process yet blocks out the UV radiation
  • Dimensions: 17.5W x 24H x 15D in. (445 x 610 x 381 mm)
  • 1 year warranty

Specification

Variable Speed

10 to 18 RPM

Heating Element

1200 Watts

Operation Range

Ambient to 99.9oC

Temperature Controller Accuracy

+0.1OC

Temperature Display

LED

Temperature Stability

Inside Oven: +0.3oC to 68oC
Inside Bottles: +0.1oC to 68oC

Temperature Uniformity

Inside Oven: +0.1oC to 68oC
Inside Bottles: +0.1oC to 68oC

Interior Dimensions

14"W x 10.75"H x 10.75:D 
(356 x 273 x 273mm)

Bottle Capacity

Twenty (20) 35 x 150 mm
Ten (10) 35 x 300 mm
Four (4) 70 x 300 mm 
(with separate Carousel Kit)

Power

12V AC/DC from 115V/60Hz or 230V/50Hz

Crosslinker Specifications (HL-2000 HybriLinker only)

Pre-set or user-selected UV energy or UV time exposure settings

Maximum UV energy setting of 999,900 microjoules per cm2

LED displays time or energy

Internal interlocking safety system

Tactile membrane switch keypad

Five 8-watt, 254nm shortwave dual bipin discharge type tubes

Interior Dimensions: 11"W x 3.7"H x 11.5" D (280 x 95 x 292mm)
Item#:
95-0031-01
Your Price:
7325.45
Each
20 x 25cm
Gel Size: 20cm(W) x 25cm(L)
Buffer volume: 1200ml

The IB56000 HR2025 is a high resolution system ideal for separation of nucleic acids  in agarose gels. It is also designed for rapid screening of PCR fragments, RFLP analysis or 
plasmid screening, field inversion, genomic DNA separations and electrophoresis  procedures requiring recirculation. Constructed of 3/8" cast acrylic to provide years of 
trouble-free service. Withstands high voltage and high temperatures and will not warp or crack like molded units do.

This System Comes Complete with:

  • Buffer Tank
  • Patented Vented Lid
  • IB56020 UVT Casting Tray
  • IB56030 Casting Fixture
  • Two, 2.0mm x 20-tooth combs
  • Power Cords
  • Bubble Level
  • Buffer Ports
  • 4 year warranty

 

Specifications:

Unit Dimensions: 8.3cm(H) x 26.7cm(W) x 39.4cm(L)

Gel Dimensions: 20cm x 25cm

Maximum sample capacity: 160 samples (4 combs, 40 samples each)

Buffer Capacity: 1200ml

Distance between electrodes: 35cm
Item#:
ASPCRELCTP5
25 x 25cm
Gel Size: 25cm(W) x 25cm(L)
Buffer volume: 1500ml

This high throughput system is designed to complete analysis of up to 200 samples 
from microtiter plates and is ideal for running all types of precast gels. The large format 
can run gels in less than 30 minutes.

 

Specifications:

Unit Dimensions: 8.3cm(H) x 26.7cm(W) x 39.4cm(L)

Gel Dimensions: 25cm x 25cm

Maximum sample capacity: 300 samples (6 combs, 50 samples each)

Buffer Capacity: 1500ml

Distance between electrodes: 35cm

This System Comes Complete with:

  • Buffer Tank
  • Patented Vented Lid
  • IB57020 UVT Casting Tray
  • IB57030 Casting Fixture
  • Four, 2.0mm x 50-tooth combs
  • Power Cords
  • Bubble Level
  • Buffer Ports
  • 4 year warranty
Item#:
ASPCRELCTP4
10cm x 15cm

Gel Size: 10cm(W) x 15cm(L)
Buffer volume: 500ml

The MP-1015 is the ideal Multi-Purpose gel box for both extended high resolution, linear runs, and economical quick screens. The MP-1015 contains buffer recirculation ports for long runs requiring recirculation. The MP-1015 is constructed of 3/8" thick, cast acrylic, to withstand high voltages and temperature extremes. Unlike single piece molded units, the MP1015 will NOT warp or crack during extended use. This unit is designed to provide years of continuous operation.

Specifications:

Construction: Buffer tank is constructed of 3/8" thick, cast acrylic. Casting tray bottom is constructed of UV transmittance cast acrylic to allow viewing of the gel with a UV light source

Unit Dimensions: 7cm(H) x 16.5cm(W) x 30.5cm(L) Gel Dimensions: 10cm x 15cm

Shipping weight: 4.5 Lbs.

Distance between the electrodes: 27cm

Maximum Sample Capacity: 80 samples (4 combs, 20 samples each)

Buffer Capacity: 500ml

The maximum suggested applied voltage for the electrophoresis of DNA in agarose gels using the IB53000-MP1015 is 140 volts. In a 1% TBE gel, this translates into a run time of approx. 1 hour. Lower voltages can be used of course, and as a general rule a 70 volt run will take twice as long as a 140 volt run. Higher voltages can be used to decrease run time however, higher voltages can produce excessive heat and decrease sample resolution.

Complete System Includes:

  • Buffer Tank

  • Patented Vented Lid

  • IB53020 UVT Casting Tray

  • IB53030 Casting Fixture

  • Two, 2.0mm x 16-tooth combs

  • Power Cords

  • Bubble Level

  • Buffer ports

  • 4 year Warranty
Item#:
ASPCRELCTP3
7x10cm

Gel Size: 7cm(W) x 10cm(L)
Buffer volume: 250ml

Designed for Quick Screening and rapid, linear separation of nucleic acids. The QS710 requires 
minimal set-up and provides excellent band resolution and consistent lane-to-lane mobility 
for accurate results. A versatile system great for applications requiring quick screens such as 
fragment analysis and recombinant screening.

 

Specifications:

Construction: Buffer tank is constructed of 3/8" cast acrylic.

Casting Tray bottom is constructed of UV Transmittance cast acrylic to
allow viewing of gel with a UV light source

Unit Dimensions: 6.4cm(H) x 11.4cm(W) x 22.2cm(L)

Gel Dimensions: 7cm x 10cm

Distance between the electrodes = 19.3cm

Maximum sample capacity = 30 samples (2 combs / 15 samples ea)

Buffer Capacity = 250ml

Shipping weight = 3.0 lbs.

The maximum suggested applied voltage for the electrophoresis of DNA in agarose gels using the QS710 is 100 volts. In a 1% TBE gel, this translates into a run time of approx. 1 hour. Lower voltages may be used, of course, and as a general rule, a 50 volt setting will take twice as long as a 100 volt setting.

The suggested run parameters for the electrophoresis of RNA in agarose gels containing formaldehyde are 60 - 80 volts.

Complete System Includes:

  • Buffer Tank
  • Patented Vented Lid
  • IB51020 UVT Casting Tray
  • IB51025 Casting Fixture
  • Two, 1.5mm x 8-tooth combs
  • Power Cords
  • Bubble Level
  • 4 year warranty
Item#:
ASPCRELCTP2
5x7cm

The IBI "Lab-Pal" has been designed with the teaching lab in mind. The IB50000 utilizes  two glass slides, measuring 2" x 3", for casting the agarose. The small glass slide 
format is ideal for minimizing agarose use while still being an excellent teaching tool for students just learning about the separation of DNA molecules. 

Agarose slides can be cast days in advance and stored in buffer for later use. This helps maximize  class time

System Includes:

  • Vented lid
  • Dual casting fixture
  • 4 glass casting slides
  • (2) 1.5mm x 5-tooth combs
  • Bubble level
  • Power cords
  • 4 year warranty
Item#:
ASPCRELCTP1
Accuris qMAX SYBR Green Bulk Packaging

Supplied as a ready-to-use 2X master mix, qMax Green has been engineered for high sensitivity, fast cycling and excellent reproducibility.
  • Superior sensitivity and fast cycling with exceptional results.
  • Ideal for low copy number templates.
  • Early Ct values and detection across a broad dynamic range.
  • Ready to use 2x mastermix.
  • Includes Accuris Hot Start Taq Polymerase for greater specificity and accuracy.
Supplied as a ready-to-use 2X master mix, qMax Green has been engineered for high sensitivity, fact cycling and excellent reproducibility. Accuris Hot Start Polymerase provides accurate PCR of a variety of templates including low copy number and difficult sequences, while the proprietary qMax Green intercalating dye exhibits higher fluorescence and lower PCR inhibition than other popular green dyes.

These two components are supported by a specially formulated buffer with an exacting combination of salts, PCR enhancers, stabilizers and pH that results in earlier Ct values and a high specificity across a broad dynamic range.

Bulk Packaging:
To accommodate the requirements of high throughput users, Accuris is now  offering their full line of qPCR and RT-qPCR reagents in bulk packaging at highly discounted prices. Bottle sizes of 25 mL (2500 reactions) and 50 mL (5000 reactions) are available for qMAX SYBR Green and qMAX Probe mixes. One Step RT-qPCR kits are also available in bulk format for 2500 and 5000 reactions and are ideal for labs performing pathogen detection.

Please Note:
Low Rox and High Rox formulations are available for compatibility with all brands of qPCR cyclers.  Please specify when ordering.  
All PCR reagents ship in protective coolers with frozen gel packs.

Item#:
ASQMAXSYBR
 
PR2000 qMax Green All Product Insert
includes agarose, myGel Mini,

 

Now is the time to SAVE BIG on our complete Electrophoresis Start-Up Package.

 

Electrophoresis System Bundle



Package Includes:

  • Complete PR1MA™ myGel™ Mini system (E1101; includes gel box, power supply, and casting sets)
  • SmartDoc™ 2.0 Gel Viewing and Smart Phone Imaging System (E5001-SDB)
  • SmartGlow DNA Stain, 1.0 mL (BE4500-PS or BE4500-LD)
  • Agarose LE, 100 grams

 

Item#:
E1000-PACKAGE
Your Price:
1095.00
Each
|◀ ◀ 265 - 276 of 293 ▶ ▶|
View: