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PCR/qPCR

MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more.  If you are looking for any of these things, we can match almost all needs to all cyclers.  If you don’t find it easily contact us at tech@midsci.com and we will find it for you. 

96 or 384 we have it.  Non-Skirted, semi-skirted or full skirted, we have it.  0.1, 0.2, or 0.5 mL we have it.  A1, A12, H12, A24, P24 cuts, we have them.  Sybr© Green or probe alternative, we have it.  

Pro Tip: Colored plastics do not have any impact on DNA amplification.  But when setting up qPCR it is often recommended to use white plastics.  This will limit or prevent fluorescence refraction.  Light refraction can minimize sensitivity and consistency. 

Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips.  This will help optimize and reduce variance in the reactions.  Simple test, see the residual liquid left in tips?  This means some of the taq, dNTPs, sample, etc is left in the tip.  PR1MA tips help reduce that significantly and improve your consistency. 

Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate?  Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler.  Call us we have solutions.

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Need a great DNA Ladder at a great price? Try PR1MA!  Click here to order.

Bullseye 100bp DNA Ladder

  • Ready to use
  • Contains 11 DNA bands: 100-1500bp.
  • Clearly identifiable 500bp band as reference
  • 500ng DNA/6µl/loading
  • Easy to load
  • Stable at room temperature
  • Supplied with 6x sample loading buffer

Bullseye 100bp DNA Ladder consists of 11 DNA fragments ranging in size from 100-1500 base pairs (bp). 6µl will yield at least 30ng DNA in any single band. The intensity of the 500bp band has been increased to serve as a reference for easy identification.


Size: 1200µl
Storage: Store at -20°C.
Concentration: 500ng/6µl
Loading Buffer Composition:

10mM Tris-HCl
1mM EDTA (pH 8.0)
0.02% Bromophenol blue
0.02% Xylene cyanol
5% Glycerol

Usage: Add at least 6µl Bullseye 100bp DNA Ladder directly to wells designated for markers. You may need more than 6µl of ladder, depending on well size and level of intensity needed to visualize the bands.

Please give us a call for a sample.

Item#:
ASDNALADD1

ENDURO™ Semi Dry Blotter

  • Western, Southern, and Northern Blots
  • Rapid transfer times
  • Uniform head dispersion
  • Variable gel thickness
  • Minimal buffer volume

The ENDURO™ Semi-Dry Blotter provides great flexibility in a lab since it can be used for all types of blotting: Western, Southern, and Northern. Blotting with the ENDURO™ Semi Dry Blotter typically can be done in 15 to 30 minutes making it a fast and easy way to do all your blotting applications. Set up procedures are easy and economical with little buffer needed. The screw down lid adjusts to varying gel thicknesses and sizes while the platinum coated electrodes provide uniform pressure ensuring even transfers. To provide your lab with total blotting solutions use with our ENDURO™ 250V, 3000mA high current power supply.


Specifications

Dimensions

32.5 x 25 x 5.5 cm | 12.8 x 9.8 x 2.2 in

Buffer Volume

20 mL

Sample Capacity

4 Blots: 8 x 8.5 cm | 2 Blots: 16 x 8.5 cm | 1 Blot: 16 x 17.5 cm
Item#:
ASDRYBLOT

Semi-Dry Blotting Systems, 10cm x 10cm


Choose Galileo Insight™ semi-dry sytems for rapid and efficient molecular transfer.
Galileo semi-dry electroblotters provide quick and efficient transfer of nucleic acids and proteins from agarose or acrylamide gels. Since only the tranfer "sandwich" of gel, membrane and blotting papers must be kept wet, much less buffer is required than traditional tank transfer systems. Solid plate style electrodes (stainless steel cathode and platinum anode) assure even and complete molecular transfer.

Item#:
ASSEMIDRYELECT1010

MAIN FEATURES


ColonyDoc-It  -  Fast, Accurate Colony Counting - Innovative Colony Counter Design

Capture the Colonies

  • DigiCam 130 digital color camera with 17.9 megapixel resolution

  • Select from several light sources for illuminating a wide range of stained media

  • Slide the filter selector to one of two positions

  • A wide range of emission filters are available

  • The plate alcove accommodates pour, spread and spiral plates and filters with sizes from 33mm to 150mm

  • Capture colony sizes as small as 0.08mm

  • Generate fast automatic and accurate colony counting with detailed statistics

  • Define parameters including eight color differentiation, split or merge, filter by group or size

  • Attach the door (not shown) to create a darkroom environment when imaging colonies with GFP fluorescence


Select from Several Light Sources

When colony samples require different light sources, the ColonyDoc-It enables the easy selection of bright LED lighting. The white light and fluorescent sources visualize a wide array of bacterial, yeast and mold colonies with samples found in air, water, food and cosmetics. The blue light is used for colonies stained with green fluorescent protein (GFP). Doors (detachable) create a darkroom environment when imaging colonies with GFP. Select from:


  • Darkfield

  • Epi and transillumination white light

  • Epi blue light


High Resolution Camera

The integrated 17.9 megapixel digital color camera lets you quickly capture and see all of the details in your colony sample. The high resolution capabilities allow capture of images down to 0.08mm. No need to adjust camera buttons, since the camera is easily controlled by the software.

Colony Counter Software

The ColonyDoc-It software loads on your computer* for camera control, image capture and analysis. The software enables automatic and manual colony counting capabilities. Users can define specific counting parameters including color differentiation, splitting or merging colonies, identifying filters by group or size. Users can create templates for specific camera settings and analysis functions, allowing the same settings to be selected each time an experiment is run. 


Generate Reports

The accurate count and statistical reporting functions enable researchers to immediately identify and determine experimental parameters that are critical for colony growth and inhibition. The software generates statistics and displays the most critical information about the colony area, perimeter, average diameter and circularity. The data is easily exported into Excel.

* Computer ordered separately.
Item#:
97-0539-01
Your Price:
8949.20
Each
250W, Programmable, 4 Jacks

Our Owl EC-1000XL Compact Power Supply is simple-to-use, multi-purpose and lightweight. The supply features a power-off memory to retain settings after shut-down as well as a stackable design to save benchtop space. It also has a soft-touch key pad and 4 jacks. 3 year warranty

Max. Voltage: 1000 V
Max. Current: 500 mA
Max. Power: 250 W


Great for DNA / RNA, Protein and Blotting electrophoresis.

Item#:
EC1000XL
Your Price:
1983.00
Each
This item is discontinued
302nm, UV/White, 20x20cm,

New electronic ballast and cutting-edge construction offers:

  • Increased ultraviolet output and intensity
  • No light flicker
  • Wider intensity range with variable selection (select models)
  • Fast lamp start up
  • Universal power supply
  • Reduced electrical consumption
  • Longer tube life
  • Lighter weight
  • Improved gel visualization and gel documentation
  • 2 year warranty

Models available include:

  • 365nm longwave UV and white light
  • 302nm midrange UV and white light or
  • Combination 2UV 365nm longwave, 302nm midrange and white light
  • Easily switch among the light sources with the rocker switch on the front of the unit
  • Each UV and white light surface area measures 20 x 20cm
  • Hinged, clear UV blocking cover, included with each transilluminator, is height adjustable for access to the filter surface
  • All transilluminators carry a two-year warranty

Specifications

Light Source:
365nm or 302nm UV White Light
Watts: 8 watts
Dimensions:

 13.25D x 19.13W x 5H in. (279 x 356 x 143mm)
Height includes UV blocking cover

Note about UV: UVP refers to its midrange UV products as 302nm. Other manufacturers may say 300nm, 310nm or 312nm. The spectral output of these units is the same as UVP's 302nm.
Part Numbers
110 - 115V 230V Model Intensity Style Wavelength Filter Size
95-0415-01 95-0415-02 TLW-20 Single 365nm/White 20x20cm
two sides
95-0415-04 95-0415-05 TMW-20 Single 302nm/White 20x20cm
two sides
95-0418-01 95-0418-02 LMW-20 Single 365/302nm/White 20x20cm
two sides
Replacement Tubes
Replacement Tube 34-0056-01 8 watt, White
Replacement Tube 34-0006-01 8 watt, 365nm
Replacement Tube 34-0042-01 8 watt, 302nm
Item#:
95-0415-04
Your Price:
3043.10
Each
Transilluminator, 470nm,

UltraBright LED Transilluminator

  • Safer to use that standard UV transillumiators
    • NO UV hazard to skin and eyes.
    • NO DNA damage inherent to UV - No worries when cutting out bands for elution.
  • Easy for use and gel cutting
    • NO need to wear amber glasses.
  • Adjustable intensity: from 100% down to 50%.
  • The THINNEST blue LED illuminator in the world.
    • Strong blue LED for excitation.
    • Uniform: <5% coefficient of variance (CV) across the full filter area.
    • Compact and Light: 30 mm in height
  • Wide applications other than DNA/RNA gel visualization, such as fluorescent proteins.
    • Sensitive for a wide range of fluorphores: DNA SafeStain, GelGreen, GelRed, EthBr, SYBR® Safe, SYBR Gold, SYBR Green I® & II, SYPRO® Rudy, SYPRO® Orange, Coomassie Fluor Orange stains, and Lumitein Protein Gel Stain.

The UltraBright LED Transilluminator is ultra-bright and provides safer blue LEDs and easy gel cutting amber filter. Higher sensitivity leads to compatibility with most stains. Full size viewing surface is 160mm x 200 mm.

Item#:
LB-16
Your Price:
1639.00
Each

Accuris MyVolt™ Mini Power Supply

  • Compact and simple
  • Choose constant 100 V or 200 V output
  • Two pairs of output terminals

 

The Accuris MyVolt Mini is a compact electrophoresis power supply ideal for basic, constant voltage applications. Users can select constant 100 V or 200 V output, and the current can reach a maximum of 200 mA.

 

Two sets of output jacks are available to connect up to 2 gel rigs and these small power supplies can securely be stacked for use with additional gel rigs.


Specifications

Output Voltage100V/200V constant
Current OutputMax. 200mA
Max Wattage40W
Output Terminals2 pairs
Weight0.9 lbs / 0.4 kg
Dimensions6.25 x 4.5 x 2.3 in.
16 x 11.5 x 5.7 cm
Electrical100 - 240V, 50-60Hz

 


Item#:
ASPS2100

Wide Format Gel System 23.5 cm x 14 cm


 Horizontal Wide Format Gel System for 23.5cm x 14cm gels. Includes UVT gel tray four comb slots and end gates & (4) 50 Tooth combs, 1.5 mm thickness.
System Description

A high-capacity system in a mini-gel size, the Galileo 2314 unit enables rapid separation of large numbers of samples.

The 2314 wide format system accomodates up to 200 samples per run, for quick separation and high thru-put. Because of the wide format of the Galileo 2314 unit, the 23cm wide by 14cm long tray is non-gasketed. Gel casting can be accomplished by taping, or we recommend the G-80-WADJ-CST multi-casting unit for even quicker and more convenient gel casting. The gel tray is UV transmissible, includes an imprinted fluorescent ruler, and has 4 comb slots. Choose from a wide variety of comb options, all with micro-titer spacing for easy loading with multi-channel pipets.
System Features

The Galileo 2314 complete system includes these features:

  •     Heavy duty lower buffer chamber
  •     Interlock safety lid with attached leads
  •     Non-gasketed 23cm wide x 14cm long UV-transparent gel tray with built-in fluorescent ruler and 4 comb slots.
  •     Four 1.5mm x 50 tooth combs
  •     Opaque well visualization plate.
Item#:
ASWIDEFORGELSY23
Transilluminator, 20x20cm,

MAIN FEATURES


  • DigiCam color camera with 16 megapixel resolution

  • Lightweight, compact hood provides a compact darkroom environment, hood now includes a new UV blocking viewer window
  • Benchtop UV Transilluminator delivers back-lit UV; select from several benchtop models
  • Color printer generates instant 4" x 6" low cost, laboratory quality color prints
  • Ethidium bromide filter for fluorescent stained gels
  • Combination lock secures the camera to the darkroom

Illuminate

Illuminate stained gels with the UV fluorescence of the compact UV transilluminator. The Ethidium Bromide emission filter, positioned inside the hood, is easily interchangeable for other filter types.

Capture and View

Capture brilliant color images with the high resolution, megapixel digital color camera. View images through the viewer window.

Save and Print

Save images to the memory card (4GM minimum) or instantly print images on the laboratory quality photo printer included with the system. The camera can be connected to a PC with a USB cable(not included) for transferring images.

Select from several Benchtop UV transilluminators:

  • M-20V: 302nm UV, 20x20cm filter

  • LM-20, 302/365nm UV, 20x20cm filter

  • M-26V, 302nm UV, 21x26cm filter

  • LM-26, 302/365nm UV, 21x26cm filter

Item#:
97-0274-04
Your Price:
5651.67
Each

Minigel System 9cm x 11cm & (1) 10 and (1) 14 tooth comb


Horizontal gel electrophoresis minigel system for 9cm x 11cm minigels. Includes gasketed UVT gel tray with fluorescent ruler, and (1) 10 and (1) 14 tooth comb, 1.5 mm thickness. Comb substitutions are available. Four year manufacturer warranty.

System description

The standard 0911 mini gel system includes a RapiCast™ gasketed gel tray for convenient gel casting right in the buffer chamber.

System features

The 0911 complete system includes these features:

  •     Heavy duty lower buffer chamber
  •     Interlock safety lid with attached leads
  •     Gasketed (cast in place) 9cm wide x 11cm long UV-transparent gel tray with built-in fluorescent ruler and 2 comb slots.
  •     One 1.5mm x 10 tooth comb
  •     One 1.5mm x 14 tooth comb
Item#:
ASMINGEL9AND11S

Double sided non- cooled vertical system, 10cm x 10cm


 Reflection double sided water cooled vertical system, 10cm x 10cm with or without casting base, two pack each of plain and notched glass, one acrylic blocker plate, (2) 10-well 0.8mm thickness combs, (4) 0.8 mm spacers. Four year warranty.
System Description

Run either one or two gels simultaneously on these versatile separation systems. The 10x10cm size of the system also accomodates most pre-cast gels.

Complete double sided vertical system for running one or two gels, in a 10 x 10 cm format. Unique casting bases allows both casting and running of gels without unclamping from the upper buffer chamber, providing for safer and more convenient gel handling. In addition the 1010 device will accommodate most precast gel cassettes on the market.

Water cooling feature is included for protocols that require it. Connections are easily made by attaching standard tubing over the 3/8" barbed in/out ports provided on the device. Water may be provided by tap or other temperature controlled water source. Optional alumina backer plates may be used to facilitate cooling efficiency and uniformity, and are available in the accessories list below.
System Features

The Galileo Reflection™ 85-1010 complete system includes these features:

  •     Heavy duty upper buffer chamber with cooling port connections.
  •     Lower buffer chamber.
  •     Interlock safety lid with attached leads.
  •     Casting base.
  •     Two-pack each of plain & notched glass plates.
  •     One acrylic blocker plate (used when running only one gel).
  •     Ttwo 10-well, 0.8mm thick combs
  •     Four 0.8mm thick side spacers.

System Accessories

Choose amoung these accessories to customize each system for your specific needs.

  •     Alumina cooling plate. For use with cooling systems. This plate can be sandwiched between the gel and the inner buffer chamber to improve overall temperature uniformity.
  •     Casting base. For quick, efficient and leak-proof casting.
  •     Multiple gradient caster.
Item#:
ASDOUBSIDNCVER10
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