RT 2X Master Mix

• Up to 9kb cDNA synthesis
• Ensures sample to sample consistency
• Large RNA sample volume capacity
• Ready to use

Size: 100 rxns

RT 2X Master Mix is a proprietary, ready-to-use master mix for first-strand cDNA synthesis in a 2X
concentration. This optimized reaction mix contains ribonuclease inhibitor, dNTPs, and a balanced
concentration for oligo(dT) and random primers. The ribonuclease inhibitor effectively protects RNA
template from degradation. The oligo(dT) anneals selectively to the poly(A) tail of mRNAs and the
random primers do not require the presence of poly(A) and they are utilized for the transcription of
mRNA 5-end regions. The resultant cDNA can be directly used as template in different PCR experiments.

Kit Components:

Storage: 
Store at -20°C in a frost-free freezer.

• Application:

  • cDNA synthesis
  • Construction of cDNA libraries
  • Generation of probes for hybridization

   

  Protocol:

  1. Thaw RNA templates and all reagents on ice. Mix each solution by vortexing.
  2. Assemble the following components in a tube on ice, and mix well:

  Components	Volume	Final Conc.
  Total RNA, or	Variable	1 ng - 2 µg/rxn
  mRNA	Variable	1 pg - 2 ng/rxn
  2X Reaction Mix	10 µl	1X
  H2O	Up to 19 µl	-

  3. Heat the mixture at 65°C for 5 mins and incubate on ice for at least 1 min.
  4. Collect all components by a brief centrifugation and add 1 µl of the EasyScript RTase to the tube.
  5. Mix well and collect all the components by a brief centrifugation.
  6. Incubate the tube at room temperature for 10 min for annealing.
  7. Perform cDNA synthesis by incubating the tube for 50 min at 42°C.
  8. Stop the reaction by heating it at 85°C for 5 min.
  9. Chill on ice. The newly synthesized first-strand cDNA is ready for immediate downstream applications.

EasyScript cDNA Synthesis Kit

Application:

• First strand cDNA synthesis for PCR
• Construction of cDNA libraries
• Generation of probes for hybridization

EasyScript cDNA Synthesis Kit is a complete system for the efficient synthesis of first strand cDNA from RNA templates. The recombinant RNasin Ribonuclease Inhibitor, supplied with the kit effectively protects RNA template from degradation.

The kit is also supplied with both oligo(dT) and random primers. The oligo(dT) anneals selectively on the poly(A) tail of mRNA. Random primers do not require the presence of poly(A). Therefore, they can be used for transcription of the 5'-end regions of mRNA. Gene-specific primers may also be used with the kit. The first strand of cDNA can be directly used as a template in PCR.

EasyScript Reverse Transcriptase (RTase) within the kit is a genetically modified form of Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV). Both the EasyScript and EasyScript Plus are RNase H deficient (negative). The enzyme is purified from bacteria host as a single holoenzyme of 71 kDa with the capacity of first-strand cDNA synthesis of template up to 9kb and can be used for routine cDNA synthesis.

Kit Components:

Storage Buffer:
50mM Tris-HCl (pH 8.3), 100 mM NaCl, 0.1 mM EDTA, 5 mM DTT, 0.1% (v/v) Triton X-100, and 50% (v/v) glycerol.

Storage: 
Store at -20°C in a frost-free freezer. Multiple freezing and thawing of RNA should be avoided. Keep RNA on ice all the time. It is recommended that the first strand cDNA synthesis is carried out under conditions where RNase contamination has been eliminated.

General Protocol:

RT-PCR reactions should be assembled in a RNA-free environment. The use of "clean", automatic pipettes designated for PCR and aerosol resistant barrier tips are recommended. 
1. Thaw template RNA and all reagents on ice. Mix each solution by vortexing, and centrifuge briefly to collect residual liquid from the sides of the tubes.
2. Prepare the following reaction mixture in a PCR tube on ice:

3. Incubate at 25°C for 10 minutes if random primer is used. Omit this step if Oligo(dT) primer or sequence specific primer are used.
4. Incubate the mixture at 42°C for 60 minutes.
5. Stop the reaction by heating at 85°C for 5 minutes.
6. Chill on ice. The newly synthesized first-strand cDNA now can be used directly for PCR amplification.

Notes:
1. Isolation of poly(A)+ RNA from total RNA is not mandatory; however, doing so may improve the yield and purity of the final product.
2. RNA sample must be free of contaminating genomic DNA.
3. Unlike the oligo(dT) priming, which usually requires no optimization, the ratio of a random primer to RNA is critical in terms of the average length of cDNA synthesized in the reaction. Increasing the ratio of random primer/RNA will result in higher yield of shorter (~500 bp) cDNA, whereas decreasing this ratio will produce longer products.

4. The synthesized cDNA should be stored at -20°C.

EasyScript Reverse Transcriptase

Application:

• Synthesis cDNA froma single-stranded RNA or DNA primer extension
• Sequencing dsDNA
• cDNA library
• Template production for use in PCR
• 3'-end labeling of duplex DNA via end-filling reactions

EasyScript Reverse Transcriptase is a genetically modified form of Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV). Both the EasyScript and EasyScript Plus are RNase H deficient (negative). The enzyme is purified from bacteria h o s t as a single holoenzyme of 71 kDa with the capacity of first-strand cDNA synthesis of template up to 9kb and can be used for routine cDNA synthesis.

Kit Components:

Storage Buffer:
50mM Tris-HCl (pH 8.3), 100 mM NaCl, 0.1 mM EDTA, 5 mM DTT, 0.1% (v/v) Triton X-100, and 50% (v/v) glycerol.

Storage: 
Store at -20°C in a frost-free freezer. Multiple freezing and thawing of RNA should be avoided. Keep RNA on ice all the time. It is recommended that the first strand cDNA synthesis is carried out under conditions where RNase contamination has been eliminated.

General Protocol:

RT-PCR reactions should be assembled in a RNA-free environment. The use of "clean", automatic pipettes designated for PCR and aerosol resistant barrier tips are recommended.

1. Thaw template RNA and all reagents on ice. Mix each solution by vortexing, and centrifuge briefly to collect residual liquid from the sides of the tubes. 
2. Prepare the following reaction mixture in a PCR tube on ice:

3. Incubate at 25°C for 10 minutes if random primer is used. Omit this step if Oligo(dT) primer or sequence specific primer are used.
4. Incubate the mixture at 42°C for 60 minutes.
5. Stop the reaction by heating at 85°C for 5 minutes. 
6. Chill on ice. The newly synthesized first-strand cDNA now can be used directly for PCR amplification.

Notes:
1. Isolation of poly(A)+ RNA from total RNA is not mandatory; however, doing so may improve the yield and purity of the final product. 
2. RNA sample must be free of contaminating genomic DNA.
3. Unlike the oligo(dT) priming, which usually requires no optimization, the ratio of a randomprimer to RNA is critical in terms of the average length of cDNA synthesized in the reaction. Increasing the ratio of random primer/RNA will result in higher yield of shorter (~500 bp) cDNA, whereas decreasing this ratio will produce longer products.

4. The synthesized cDNA should be stored at -20°C.

ENDURO VE20 Vertical Gel Electrophoresis System

Enduro VE20 Vertical Electrophoresis System (20 x 20 cm), includes glass plates, 2 x 24 well combs, cooling coil and casting base

• Large format vertical gel system
• Electroblotting and gel electrophoresis in same universal gel tank
• Quick assembly system for ease of use
• Complete system allows casting of gels and running of gels at same time increasing efficiency

The ENDURO VE20 is Labnet's new large format vertical gel electrophoresis system. It distinguishes itself in its flexibility of applications and ease of gel casting and set-up requiring only 4 screws to secure 4 gels. The same gel tank can house a large format electroblotting assembly sold separately. This system is ideal for first- and second dimension SDS-PAGE, native, preparative, gradient and high-resolution nucleic acid electrophoresis.

Innovative screw-clamp technology consists of only four screws to secure up to four 20 x 20cm gels. This vertical screw clamp technology distributes pressure evenly along the height of gel rather than in the center to eliminate plate bowing and gel compression, while maintaining a leak-proof seal during casting.

NO TANK TOP ASSEMBLY
A built-in inner buffer chamber within the PAGE insert allows set up to be completed without inclusion of an upper buffer chamber.

CASTING ADVANTAGES

• Dual purpose PAGE insert eliminates time consuming transfer of glass plates between separate casting and gel running components.
• Glass plates with bonded spacers assure clean well formation, correct alignment for leak-free casting and eliminates manually aligning spacers.
• Casting stand design ensures leak-free casting even if glass plates are misaligned.

Specifications:

Bullseye 1Kb DNA Ladder, Logic

• Ready to use
• Contains 16 DNA bands: 100bp-10Kb
• 920ng DNA/6µl/loading
• Easy quantification of DNA fragments
• Stable at room temperature
• Supplied with 6x sample loading buffer

Bullseye 1Kb DNA Ladder, Logic contains 16 DNA bands ranging from 100bp to 10Kb, formulated so that each band contains an amount of DNA that correlates logically to its size, allowing the user to estimate both the size and the quantity of specific fragments at a glance. It is particularly useful for protocols such as probe labeling, DNA sequencing and optimizing insert/vector ratio in ligation reactions, where DNA concentration must be taken into consideration.

Size: 1200µl
Storage: Store at -20°C.
Concentration: 920ng/6µl

Loading Buffer Composition:

10mM Tris-HCl
1mM EDTA (pH 8.0)
0.02% Bromophenol blue
0.02% Xylene cyanol
5% Glycerol

Usage: Add 6µl of Bullseye 1Kb DNA Ladder, Logic directly to wells designated for markers, which will yield the amount as indicated in the picture. You may use more than 6µl of ladder, depending on well size and level of dye used to visualize the bands. Calculate the amount for each band according to your loading volume.

This brand is being discontinued and will only be available while supplies last.

Need a great DNA Ladders at a great price?

Try our PR1MA™ SmartCheck DNA Ladders

• A unique combination of PCR products and a number of proprietary plasmids digested with appropriate restriction enzymes to yield 11 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis.
• The DNA includes fragments ranging from 100-1,500 base pairs. The 500 and 1,500 base pair bands have increased intensity to serve as reference points.
• The approximate mass of DNA in each band is provided (0.5µg a load) for approximating the mass of DNA in comparably intense samples of similar size.

Source:

• PCR products and double-stranded DNA digested with appropriate restriction enzymes, are phenol and equilibrated to 10nM Tris-HCl (pH 8.0) and 10mM EDTA.

Range: 100-1,500 bp (DL1); 250-10,000 bp (DL5)
Number of bands: 11 (DL1); 13 (DL5)
Concentration: 100 µg/mL
Package: 50 µg/500 µL
Recommended Load: 5 µL / well
Contains orange G & xylene cyanol FF as tracking dyes. (DL1)
Contains bromophenol blue & xylene cyanol FF as tracking dyes. (DL5)
Storage:

• Store at 25°C for 6 months
• Store at 4°C for 12 months

Store at -20°C for 24 months

Bullseye 100bp DNA Ladder

• Ready to use
• Contains 11 DNA bands: 100-1500bp.
• Clearly identifiable 500bp band as reference
• 500ng DNA/6µl/loading
• Easy to load
• Stable at room temperature
• Supplied with 6x sample loading buffer

Bullseye 100bp DNA Ladder consists of 11 DNA fragments ranging in size from 100-1500 base pairs (bp). 6µl will yield at least 30ng DNA in any single band. The intensity of the 500bp band has been increased to serve as a reference for easy identification.

Size: 1200µl
Storage: Store at -20°C.
Concentration: 500ng/6µl
Loading Buffer Composition:

10mM Tris-HCl
1mM EDTA (pH 8.0)
0.02% Bromophenol blue
0.02% Xylene cyanol
5% Glycerol

Usage: Add at least 6µl Bullseye 100bp DNA Ladder directly to wells designated for markers. You may need more than 6µl of ladder, depending on well size and level of intensity needed to visualize the bands.

Please give us a call for a sample.

ENDURO™ Semi Dry Blotter

• Western, Southern, and Northern Blots
• Rapid transfer times
• Uniform head dispersion
• Variable gel thickness
• Minimal buffer volume

The ENDURO™ Semi-Dry Blotter provides great flexibility in a lab since it can be used for all types of blotting: Western, Southern, and Northern. Blotting with the ENDURO™ Semi Dry Blotter typically can be done in 15 to 30 minutes making it a fast and easy way to do all your blotting applications. Set up procedures are easy and economical with little buffer needed. The screw down lid adjusts to varying gel thicknesses and sizes while the platinum coated electrodes provide uniform pressure ensuring even transfers. To provide your lab with total blotting solutions use with our ENDURO™ 250V, 3000mA high current power supply.

Specifications

Semi-Dry Blotting Systems, 10cm x 10cm

PR1MA™ Reverse Transcriptase, RNase H -

PR1MA™ reverse transcriptase (RT) is an RNA-dependent DNA polymerase ideal for use in RT-PCR and first-strand synthesis of complementary DNA (cDNA) for generation of cDNA libraries from single-stranded RNA, DNA, or RNA:DNA hybrids.

• Decreased RNase H activity enables longer cDNA synthesis (>5 kb).
• Lacks 3’ to 5’ exonuclease activity
• Optimal temperature at 42°C
• Temperature range: 40 to 50°C
• Heat inactivation: 70°C for 20 minutes
• Storage temperature: -20°C
• 10X reaction buffer included 

Buffer composition

• 50% glycerol
• 50 mM Tris-HCl
• 50 mM KCl
• 1 mM DTT
• 0.1  mM EDTA
• pH = 7.5

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.