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PCR/qPCR

MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more.  If you are looking for any of these things, we can match almost all needs to all cyclers.  If you don’t find it easily contact us at tech@midsci.com and we will find it for you. 

96 or 384 we have it.  Non-Skirted, semi-skirted or full skirted, we have it.  0.1, 0.2, or 0.5 mL we have it.  A1, A12, H12, A24, P24 cuts, we have them.  Sybr© Green or probe alternative, we have it.  

Pro Tip: Colored plastics do not have any impact on DNA amplification.  But when setting up qPCR it is often recommended to use white plastics.  This will limit or prevent fluorescence refraction.  Light refraction can minimize sensitivity and consistency. 

Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips.  This will help optimize and reduce variance in the reactions.  Simple test, see the residual liquid left in tips?  This means some of the taq, dNTPs, sample, etc is left in the tip.  PR1MA tips help reduce that significantly and improve your consistency. 

Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate?  Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler.  Call us we have solutions.

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20 x 25cm
Gel Size: 20cm(W) x 25cm(L)
Buffer volume: 1200ml

The IB56000 HR2025 is a high resolution system ideal for separation of nucleic acids  in agarose gels. It is also designed for rapid screening of PCR fragments, RFLP analysis or 
plasmid screening, field inversion, genomic DNA separations and electrophoresis  procedures requiring recirculation. Constructed of 3/8" cast acrylic to provide years of 
trouble-free service. Withstands high voltage and high temperatures and will not warp or crack like molded units do.

This System Comes Complete with:

  • Buffer Tank
  • Patented Vented Lid
  • IB56020 UVT Casting Tray
  • IB56030 Casting Fixture
  • Two, 2.0mm x 20-tooth combs
  • Power Cords
  • Bubble Level
  • Buffer Ports
  • 4 year warranty

 

Specifications:

Unit Dimensions: 8.3cm(H) x 26.7cm(W) x 39.4cm(L)

Gel Dimensions: 20cm x 25cm

Maximum sample capacity: 160 samples (4 combs, 40 samples each)

Buffer Capacity: 1200ml

Distance between electrodes: 35cm
Item#:
ASPCRELCTP5
25 x 25cm
Gel Size: 25cm(W) x 25cm(L)
Buffer volume: 1500ml

This high throughput system is designed to complete analysis of up to 200 samples 
from microtiter plates and is ideal for running all types of precast gels. The large format 
can run gels in less than 30 minutes.

 

Specifications:

Unit Dimensions: 8.3cm(H) x 26.7cm(W) x 39.4cm(L)

Gel Dimensions: 25cm x 25cm

Maximum sample capacity: 300 samples (6 combs, 50 samples each)

Buffer Capacity: 1500ml

Distance between electrodes: 35cm

This System Comes Complete with:

  • Buffer Tank
  • Patented Vented Lid
  • IB57020 UVT Casting Tray
  • IB57030 Casting Fixture
  • Four, 2.0mm x 50-tooth combs
  • Power Cords
  • Bubble Level
  • Buffer Ports
  • 4 year warranty
Item#:
ASPCRELCTP4
10cm x 15cm

Gel Size: 10cm(W) x 15cm(L)
Buffer volume: 500ml

The MP-1015 is the ideal Multi-Purpose gel box for both extended high resolution, linear runs, and economical quick screens. The MP-1015 contains buffer recirculation ports for long runs requiring recirculation. The MP-1015 is constructed of 3/8" thick, cast acrylic, to withstand high voltages and temperature extremes. Unlike single piece molded units, the MP1015 will NOT warp or crack during extended use. This unit is designed to provide years of continuous operation.

Specifications:

Construction: Buffer tank is constructed of 3/8" thick, cast acrylic. Casting tray bottom is constructed of UV transmittance cast acrylic to allow viewing of the gel with a UV light source

Unit Dimensions: 7cm(H) x 16.5cm(W) x 30.5cm(L) Gel Dimensions: 10cm x 15cm

Shipping weight: 4.5 Lbs.

Distance between the electrodes: 27cm

Maximum Sample Capacity: 80 samples (4 combs, 20 samples each)

Buffer Capacity: 500ml

The maximum suggested applied voltage for the electrophoresis of DNA in agarose gels using the IB53000-MP1015 is 140 volts. In a 1% TBE gel, this translates into a run time of approx. 1 hour. Lower voltages can be used of course, and as a general rule a 70 volt run will take twice as long as a 140 volt run. Higher voltages can be used to decrease run time however, higher voltages can produce excessive heat and decrease sample resolution.

Complete System Includes:

  • Buffer Tank

  • Patented Vented Lid

  • IB53020 UVT Casting Tray

  • IB53030 Casting Fixture

  • Two, 2.0mm x 16-tooth combs

  • Power Cords

  • Bubble Level

  • Buffer ports

  • 4 year Warranty
Item#:
ASPCRELCTP3
7x10cm

Gel Size: 7cm(W) x 10cm(L)
Buffer volume: 250ml

Designed for Quick Screening and rapid, linear separation of nucleic acids. The QS710 requires 
minimal set-up and provides excellent band resolution and consistent lane-to-lane mobility 
for accurate results. A versatile system great for applications requiring quick screens such as 
fragment analysis and recombinant screening.

 

Specifications:

Construction: Buffer tank is constructed of 3/8" cast acrylic.

Casting Tray bottom is constructed of UV Transmittance cast acrylic to
allow viewing of gel with a UV light source

Unit Dimensions: 6.4cm(H) x 11.4cm(W) x 22.2cm(L)

Gel Dimensions: 7cm x 10cm

Distance between the electrodes = 19.3cm

Maximum sample capacity = 30 samples (2 combs / 15 samples ea)

Buffer Capacity = 250ml

Shipping weight = 3.0 lbs.

The maximum suggested applied voltage for the electrophoresis of DNA in agarose gels using the QS710 is 100 volts. In a 1% TBE gel, this translates into a run time of approx. 1 hour. Lower voltages may be used, of course, and as a general rule, a 50 volt setting will take twice as long as a 100 volt setting.

The suggested run parameters for the electrophoresis of RNA in agarose gels containing formaldehyde are 60 - 80 volts.

Complete System Includes:

  • Buffer Tank
  • Patented Vented Lid
  • IB51020 UVT Casting Tray
  • IB51025 Casting Fixture
  • Two, 1.5mm x 8-tooth combs
  • Power Cords
  • Bubble Level
  • 4 year warranty
Item#:
ASPCRELCTP2
5x7cm

The IBI "Lab-Pal" has been designed with the teaching lab in mind. The IB50000 utilizes  two glass slides, measuring 2" x 3", for casting the agarose. The small glass slide 
format is ideal for minimizing agarose use while still being an excellent teaching tool for students just learning about the separation of DNA molecules. 

Agarose slides can be cast days in advance and stored in buffer for later use. This helps maximize  class time

System Includes:

  • Vented lid
  • Dual casting fixture
  • 4 glass casting slides
  • (2) 1.5mm x 5-tooth combs
  • Bubble level
  • Power cords
  • 4 year warranty
Item#:
ASPCRELCTP1
Accuris qMAX SYBR Green Bulk Packaging

Supplied as a ready-to-use 2X master mix, qMax Green has been engineered for high sensitivity, fast cycling and excellent reproducibility.
  • Superior sensitivity and fast cycling with exceptional results.
  • Ideal for low copy number templates.
  • Early Ct values and detection across a broad dynamic range.
  • Ready to use 2x mastermix.
  • Includes Accuris Hot Start Taq Polymerase for greater specificity and accuracy.
Supplied as a ready-to-use 2X master mix, qMax Green has been engineered for high sensitivity, fact cycling and excellent reproducibility. Accuris Hot Start Polymerase provides accurate PCR of a variety of templates including low copy number and difficult sequences, while the proprietary qMax Green intercalating dye exhibits higher fluorescence and lower PCR inhibition than other popular green dyes.

These two components are supported by a specially formulated buffer with an exacting combination of salts, PCR enhancers, stabilizers and pH that results in earlier Ct values and a high specificity across a broad dynamic range.

Bulk Packaging:
To accommodate the requirements of high throughput users, Accuris is now  offering their full line of qPCR and RT-qPCR reagents in bulk packaging at highly discounted prices. Bottle sizes of 25 mL (2500 reactions) and 50 mL (5000 reactions) are available for qMAX SYBR Green and qMAX Probe mixes. One Step RT-qPCR kits are also available in bulk format for 2500 and 5000 reactions and are ideal for labs performing pathogen detection.

Please Note:
Low Rox and High Rox formulations are available for compatibility with all brands of qPCR cyclers.  Please specify when ordering.  
All PCR reagents ship in protective coolers with frozen gel packs.

Item#:
ASQMAXSYBR
 
PR2000 qMax Green All Product Insert
includes agarose, myGel Mini,

 

Now is the time to SAVE BIG on our complete Electrophoresis Start-Up Package.

 

Electrophoresis System Bundle



Package Includes:

  • Complete PR1MA™ myGel™ Mini system (E1101; includes gel box, power supply, and casting sets)
  • SmartDoc™ 2.0 Gel Viewing and Smart Phone Imaging System (E5001-SDB)
  • SmartGlow DNA Stain, 1.0 mL (BE4500-PS or BE4500-LD)
  • Agarose LE, 100 grams

 

Item#:
E1000-PACKAGE
Your Price:
1095.00
Each

Accuris™ UV transilluminator


  • 302nm midrange UV wavelength (optimal for EtBr excitation)
  • Dual intensity settings (50% or 100%)
  • Hinged UV Safety cover
  • Use with SmartDocTM enclosure (E5000-SD) for gel imaging with a smart phone
  • Compatible DNA stains: EtBr, SmartGlow™ LD and PS stain, Gel Red, Gel Green, SYBR® Green, SYBR® safe, and Diamond™ Nucleic acid Dye
  • 2-yr warranty

This new UV transilluminator features a space-saving design while having 16 x 19 cm UV viewing surface to accommodate large gels or multiple smaller gels. The illuminated surface is comprised of a special black glass which is easy to clean, durable and scratch resistant.


Furthermore, an array of high intensity UV bulbs coupled with an optimized filter provides even illumination across the viewing surface and allows detection of small sample quantities. The dual intensity switch can be used to lower the UV intensity to 50% to reduce DNA damage during long exposure times, or switch to high intensity (100%) for viewing weak fluorescent signals or for photo documentation.


The acrylic UV blocking cover allows safe viewing of samples and has two friction hinges on the front edge to allow angling of the cover for gel access. The cover can be positioned to 90° (straight up) to fit a gel imaging hood, such as the SmartDoc™ imaging enclosure (E5000-SD).

Technical Specifications


Light Source

UV

Wavelength

302 nm

Viewing Surface

16 x 19 cm

Ext. Dimensions

10.5 x 13 x 5.5 in | 26.5 x 32.5 x 14 cm

Electrical

120 or 230V, 50-60Hz

Warranty

2 years
Item#:
ASACCURISUVTRANAT
COVID-19 genesig® Real Time PCR assay | 96 Tests
 
  • Lyophilized components to ship without cold chain
  • Highly specific detection profile and high priming efficiency
  • Rapid detection of COVID-19 and does not detect other related coronavirus strains


Design of our COVID-19 was an intricate process using a dedicated team of expert bioinformaticians. Finding the right sequence is critical for not only assay specificity, but also sensitivity which can be inhibited by primer-dimer formation. These ultimately reduce the assay's ability to detect SARS-CoV-2 accurately. However, the right design will mitigate these risks and ensure a specific, sensitive and accurate test, as we have done.


Our surveillance program is in place to track the mutation of the virus. Our expert bioinformaticians analyze daily published virus sequences to ensure our test maintains a 100% detection profile. Each new mutation presents a risk to any COVID-19 kit detection and false negatives without surveillance programs in place. As of the 17th July 2020 our COVID-19 test has a 100% detection profile with the 42,655 full length, good quality SARS-CoV-2 sequences published on the GISAID-EpiCoV database.


Ensuring your COVID-19 test is stable during transit and storage is vital to prevent reagent degradation. Within our COVID-19 genesig® Real Time PCR assay we provide oasig components that are provided freeze dried which stabilizes the active components. This enables the reagents to be shipped at ambient temperature. This simplifies the logistics of shipping and is cost effective. If you're in a sophisticated laboratory or a mobile field hospital we can supply complete qPCR packages to your door quickly and cheaply via standard shipping methods without the need for dry ice or a cold chain of any sort.


The COVID-19 genesig® Real Time PCR assay was granted Emergency Use Authorization Only by the United States Food and Drug Administration (FDA).

Item#:
ASCODVID19
  • Increased ultraviolet output and intensity
  • No light flicker
  • Wider intensity range with variable selection (select models)
  • Fast lamp start up
  • Universal power supply
  • Reduced electrical consumption
  • Longer tube life
  • Lighter weight
  • Improved gel visualization and gel documentation
  • UVP's patented 3UV Benchtop Transilluminator provides the selection of 365nm, 302nm and 254nm in one compact unit
  • 2UV models offer 302nm and 365nm UV in one model
  • Easily view gels under 365nm longwave UV for extended periods of time avoiding photonicking damage. Researchers can use the 302nm midrange to increase the fluorescence for photodocumentation. Shortwave 254nm UV is used to irradiate samples
  • All Benchtop Transilluminator models are compact, lightweight units
  • These 8-watt models provide a low cost alternative to the High Performance UV Transilluminators
  • A variety of filter area work surface sizes are available
  • Hinged, clear UV blocking cover, included with each transilluminator, is adjustable for access to the filter surface
  • 2 year warranty

WARNING: Transilluminators are powerful sources of ultraviolet radiation which can cause serious damage to unprotected eyes and skin. Read the ultraviolet Caution Information before operating any transilluminators.

* Except M-10 which has standard ballast, 115V unit.

Intensity :

  • Select models offer single intensity other models include variable intensity. Refer to the part numbers tab for information.
  • Variable intensity settings-High/Medium/Low 
  • High setting allows for UV excitation of fluorophores on gels for routine photography. Medium is excellent for viewing and quick single-band excision. Low setting allows for positioning and preparation of gels, excising multiple bands, and focusing for photography.

UV Source: Refer to the Part Numbers tab for specific wavelengths for each model

Watts: 8 watts, except M-10 which is 6 watts

Dimensions:

  • 3UV Models: 11D x 14W x 5.4H in. (279 x 356 x 137mm) 
  • 2UV Models: 11D x 14W x 4.8H in. (279 x 356 x 122mm) 
  • Single UV/Variable Intensity Models (Except M-10): 11D x 14W x 4.8H in. (279 x 356 x 122mm) 
  • M-10: 7.25D x 10.25W x 4.5H in. (184 x 260 x 114mm) 
  • Height includes UV blocking cover (all models)

Note about UV: UVP refers to its midrange UV products as 302nm. Other manufacturers may say 300nm, 310nm or 312nm. The spectral output of these units is the same as UVP's 302nm.

Options
  • Gel Tools
  • Converter Plates
  • BioImaging Systems
95-0457-01 (115V) M-26E Single 302nm 8 watt 21x26cm
 Single UV / Variable Intensity
95-0456-01 M-15V Variable 302nm 8 watt 15x15cm
95-0452-01 M-20V Variable 302nm 8 watt 20x20cm
95-0458-01 M-26V Variable 302nm 8 watt 21x26cm
95-0413-01 M-26XV Variable 302 8 watt 25x26cm
 2UV / Single Intensity
95-0449-01 LM-20 Single 302/365nm 8 watt 20x20cm
95-0459-01 LM-26 Single 302/365nm 8 watt 21x26cm
 3UV / Single Intensity
95-0417-01 LMS-20 Single 254/302/365nm 8 watt 20x20cm
95-0414-01 LMS-26 Single 254/302/365nm 8 watt 21x26cm
Item#:
ASPCRUVEQ7
  • Increased ultraviolet output and intensity
  • No light flicker
  • Wider intensity range with variable selection (select models)
  • Fast lamp start up
  • Universal power supply
  • Reduced electrical consumption
  • Longer tube life
  • Lighter weight
  • Improved gel visualization and gel documentation
  • UVP's patented 3UV Benchtop Transilluminator provides the selection of 365nm, 302nm and 254nm in one compact unit
  • 2UV models offer 302nm and 365nm UV in one model
  • Easily view gels under 365nm longwave UV for extended periods of time avoiding photonicking damage. Researchers can use the 302nm midrange to increase the fluorescence for photodocumentation. Shortwave 254nm UV is used to irradiate samples
  • All Benchtop Transilluminator models are compact, lightweight units
  • These 8-watt models provide a low cost alternative to the High Performance UV Transilluminators
  • A variety of filter area work surface sizes are available
  • Hinged, clear UV blocking cover, included with each transilluminator, is adjustable for access to the filter surface
  • 2 year warranty

WARNING: Transilluminators are powerful sources of ultraviolet radiation which can cause serious damage to unprotected eyes and skin. Read the ultraviolet Caution Information before operating any transilluminators.

* Except M-10 which has standard ballast, 115V unit.

Intensity :

  • Select models offer single intensity other models include variable intensity. Refer to the part numbers tab for information.
  • Variable intensity settings-High/Medium/Low 
  • High setting allows for UV excitation of fluorophores on gels for routine photography. Medium is excellent for viewing and quick single-band excision. Low setting allows for positioning and preparation of gels, excising multiple bands, and focusing for photography.

UV Source: Refer to the Part Numbers tab for specific wavelengths for each model

Watts: 8 watts, except M-10 which is 6 watts

Dimensions:

  • 3UV Models: 11D x 14W x 5.4H in. (279 x 356 x 137mm) 
  • 2UV Models: 11D x 14W x 4.8H in. (279 x 356 x 122mm) 
  • Single UV/Variable Intensity Models (Except M-10): 11D x 14W x 4.8H in. (279 x 356 x 122mm) 
  • M-10: 7.25D x 10.25W x 4.5H in. (184 x 260 x 114mm) 
  • Height includes UV blocking cover (all models)

Note about UV: UVP refers to its midrange UV products as 302nm. Other manufacturers may say 300nm, 310nm or 312nm. The spectral output of these units is the same as UVP's 302nm.

Options
  • Gel Tools
  • Converter Plates
  • BioImaging Systems
95-0457-01 (115V) M-26E Single 302nm 8 watt 21x26cm
 Single UV / Variable Intensity
95-0456-01 M-15V Variable 302nm 8 watt 15x15cm
95-0452-01 M-20V Variable 302nm 8 watt 20x20cm
95-0458-01 M-26V Variable 302nm 8 watt 21x26cm
95-0413-01 M-26XV Variable 302 8 watt 25x26cm
 2UV / Single Intensity
95-0449-01 LM-20 Single 302/365nm 8 watt 20x20cm
95-0459-01 LM-26 Single 302/365nm 8 watt 21x26cm
 3UV / Single Intensity
95-0417-01 LMS-20 Single 254/302/365nm 8 watt 20x20cm
95-0414-01 LMS-26 Single 254/302/365nm 8 watt 21x26cm
Item#:
ASPCRUVEQ5

New electronic ballast and cutting-edge construction offers:

  • Increased ultraviolet output and intensity
  • No light flicker
  • Wider intensity range with variable selection (select models)
  • Fast lamp start up
  • Universal power supply
  • Reduced electrical consumption
  • Longer tube life
  • Lighter weight
  • Improved gel visualization and gel documentation
  • High performance 25-watt UV tubes for high fluorescence and sensitivity on stained gels
  • Stainless steel cover frame provide an easy clean surface and reduces cross contamination of samples
  • Uniform, brilliant back-lit fluorescence illumination is enhanced with UVP's unique background uniformity screen and UVG long-life filter
  • Uniformity screen is available on units with midrange or longwave UV
  • The transilluminator's powerful cooling fan maintains a low filter surface temperature to prevent sample damage and maintains a constant UV intensity
  • The removable and adjustable protective UV blocking cover is included with each transilluminator
  • Transilluminators carry a two-year warranty
  • 2 year warranty

WARNING: Transilluminators are powerful sources of ultraviolet radiation which can cause serious damage to unprotected eyes and skin. Read the ultraviolet Caution Information before operating any transilluminators.

Intensity:
  • Select models offer single intensity other models include variable intensity. Refer to the part numbers tab for information. 
  • Variable intensity settings - High/Medium/Low 
  • High setting allows for UV excitation of fluorophores on gels for routine photography. Medium is excellent for viewing and quick single-band excision. Low setting allows for positioning and preparation of gels, excising multiple bands, and focusing for photography.

UV Source: 254nm, 302nm or 365nm

Watts: 25 watts

Dimensions: All Benchtop models : 13.25D x 19.13W x 5.63H in. 

  • Height includes UV blocking cover
  • Weight: 21 lb. (9.6 kg)

Note about UV: UVP refers to its midrange UV products as 302nm. Other manufacturers may say 300nm, 310nm or 312nm. The spectral output of these units is the same as UVP's 302nm.

Part Numbers
100-115V 230V Model Intensity Style Wavelength Filter Size
Variable UV Intensity
95-0420-01 95-0420-02 TFL-40V Variable 365nm 20x40cm
95-0423-01 95-0423-02 TFM-20V Variable 302nm 20x20cm
95-0422-01 95-0422-02 TFM-26V Variable 302nm 21x26cm
95-0424-01 95-0424-02 TFM-30V Variable 302nm 25x30cm
95-0421-01 95-0421-02 TFM-40V Variable 302nm 20x40cm
95-0427-01 95-0427-02 TFS-20V Variable 254nm 20x20cm
95-0428-01 95-0428-02 TFS-26V Variable 254nm 21x26cm
95-0429-01 95-0429-02 TFS-30V Variable 254nm 25x30cm
95-0430-01 95-0430-02 TFS-40V Variable 254nm 20x40cm
2UV
95-0431-01 95-0431-02 TFML-20 Single 302/365nm 20x20cm
95-0432-01 95-0431-02 TFML-30 Single 302/365nm 25x30cm
95-0425-01 95-0425-02 TFML-26 Single 302/365nm 21x26cm
95-0426-01 95-0426-02 TFML-40 Single 302/365nm 20x40cm
Replacement Tubes
Replacement Tube 34-0073-01 25-watt, 254nm
Replacement Tube 34-0072-01 25-watt, 302nm
Replacement Tube 34-0060-01 25-watt, 365nm
Item#:
ASPCRUVEQ6
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