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PCR/qPCR

MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more.  If you are looking for any of these things, we can match almost all needs to all cyclers.  If you don’t find it easily contact us at tech@midsci.com and we will find it for you. 

96 or 384 we have it.  Non-Skirted, semi-skirted or full skirted, we have it.  0.1, 0.2, or 0.5 mL we have it.  A1, A12, H12, A24, P24 cuts, we have them.  Sybr© Green or probe alternative, we have it.  

Pro Tip: Colored plastics do not have any impact on DNA amplification.  But when setting up qPCR it is often recommended to use white plastics.  This will limit or prevent fluorescence refraction.  Light refraction can minimize sensitivity and consistency. 

Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips.  This will help optimize and reduce variance in the reactions.  Simple test, see the residual liquid left in tips?  This means some of the taq, dNTPs, sample, etc is left in the tip.  PR1MA tips help reduce that significantly and improve your consistency. 

Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate?  Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler.  Call us we have solutions.

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Large Tank Blotter with two cassettes, 20 x 20cm

  • Designed to optimally transfer very large and difficult proteins.
  • Reproducible transfer results thanks to the plate electrodes made
  • of platinum-plated titanium (anode) and stainless steel (cathode).
  • Efficient cooling system: Water circulation system with alumina
  • ceramic wall located at the chamber base for effective heat
  • removal.
  • Safety lid: asymmetrical configuration of electrical contacts
  • prevents mismatching of electrodes.

Item#:
ASLARGETANKBLOT2020

Double sided water cooled vertical system, 16cmW x 14cm


 With its 16 cm wide x 14 cm tall format, the Reflection 1614 large-format, dual-gel vertical electrophoresis unit is great for separating complex protein samples. Run either one or two electrophoresis gels in this vertical gel box that provides both long run lengths and high separation capacity. With Galileo's unique casting base, users are able to both cast and run gels without ever having to unclamp the upper buffer chamber. This allows for safer and more convenient gel handling, especially for those who may be prone to ripping their gels between the two activities.

This vertical gel box includes a water cooling feature for electrophoresis protocols that require lower temperature. This water can be provided by the tap or other temperature controlled source and is easily connected by attaching standard tubing over the 3/8" barbed in/out ports on the device. Users may also facilitate cooling efficiency and uniformity using Galileo's optional alumina backer plates (see accessories below).

System Features

Each Reflection 85-1614 vertical system includes the following components:

  •     Heavy duty upper buffer chamber with cooling port connections
  •     Lower buffer chamber
  •     Interlock safety lid with attached leads
  •     Casting base
  •     Four-pack each of plain and notched glass plates
  •     One acrylic blocker plate (used when only one gel is being run)
  •     Eight 1.5mm thick side spacers
  •     Four combs of your choice

System Accessories

  •     Alumina cooling plate for use with cooling systems. This plate can be sandwiched between the gel and the inner buffer chamber to improve overall temperature uniformity.
  •     Casting base for quick, efficient and leak-proof gel casting.
Item#:
ASDOUBLEWATVERT16
470nm,

UltraSlim® LED Illuminator

  • Safer to use that standard UV transillumiators
    • NO UV hazard to skin and eyes.
    • NO DNA damage inherent to UV - No worries when cutting out bands for elution.
  • Easy for use and gel cutting
    • NO need to wear amber glasses.
  • The THINNEST blue LED illuminator in the world.
    • Strong blue LED for excitation.
    • Uniform: <10% coefficient of variance (CV) in the central viewing area.
    • Compact and Light: 30 mm in height
  • Wide applications other than DNA/RNA gel visualization, such as fluorescent proteins.
    • Sensitive for a wide range of fluorphores: DNA SafeStain, GelGreen, GelRed, EthBr, SYBR® Safe, SYBR Gold, SYBR Green I® & II, SYPRO® Rudy, SYPRO® Orange, Coomassie Fluor Orange stains, and Lumitein Protein Gel Stain.

The UltraSlim® LED Illuminator is designed for viewing stained gels on the laboratory bench, or within gel documentation systems. Safer lighting from the LED source inside the instrument produces blue light with a narrow emission peak centered at approximately 470 nm, which is more effective for the excitation of most nucleic acid and protein stains. Viewing surface is 120mm x 70mm.

 

Item#:
SLB-01
Your Price:
1001.00
Each

Minigel System 7cm x 8 cm & (1) 6 and (1) 10 tooth comb


Perfectly sized for rapid analysis of small analytes like PCR and RE fragments. This unit features a RapidCast gasketed tray for quick in-unit gel casting.

System Description

Horizontal gel electrophoresis minigel system for 7cm x 8cm minigels. Includes gasketed UVT gel tray with fluorescent ruler, and (1) 6 and (1) 10 tooth comb, 1.5 mm thickness. Comb substitutions are available. Four year manufacturer warranty.

The standard 0708 mini gel system includes a RapidCast™ gasketed gel tray for convenient gel casting right in the buffer chamber.

System features

The 0708 complete system includes these features:

  •     Heavy duty lower buffer chamber
  •     Interlock safety lid with attached leads
  •     Gasketed (cast in place) 7cm wide x 8cm long UV-transparent gel tray with built-in fluorescent ruler and 2 comb slots.
  •     One 1.5mm x 6 tooth comb
  •     One 1.5mm x 10 tooth comb
Item#:
ASMINGEL7AND8S

Horizontal Electrophoresis System, 20 cm x 25 cm

Horizontal Large Gel Electrophoresis System for 20cmW x 25cm gel. Includes 20cm x 25cm UVT Gel Tray w/ end gates &amp; (1) 16, (1) 24 and (1) 36 Tooth, 1.5 mm thickness. Includes four year warranty.

 The 81-2025 is a medium capacity system. With a maximum run length of 25cm, the unit can provide high resolution of complex analytes. Or, fitted with maximum capacity combs in all of the tray's 6 comb slots, the unit can handle up to 168 samples. The 2025 features the Galileo ExpressCast gel casting system for quick, tape-free gel casting and includes a built-in bubble level and leveling screws for convenient level adjustment. You may choose from a wide variety of comb options to customize your order.

  •     Heavy duty lower buffer chamber
  •     Interlock safety lid with attached leads
  •     End gated 20cm wide x 25cm long ExpressCast™ UV-transparent gel tray with built-in fluorescent ruler and 6 comb slots.
  •     One 1.5mm x 16 tooth combs, one 1.5mm x 24 tooth combs, one 1.5mm x 36 tooth combs
Galileo automatic buffer recirculation reduces detrimental ion and pH gradients in your buffer, encourages even migration of nucleic acids, and is ideal for long running gels. Buffer recirculation can also prevent glyoxalated RNA from dissociating in the presence of ion gradients.

The system is simple and self contained. A trapping system captures hydrogen bubbles created by electrolysis of the buffer at the cathode (-) end of the unit. As these bubbels move up the canted recirculation tube, they carry buffer along with them and provide a gentle and reliable recirculation system. This system is self regulating and no additional tubing or pumps are required.
Item#:
ASHORIZELCTSY20

Semi-Dry Blotting Systems, 20cm x 20cm

Choose Galileo Insight™ semi-dry sytems for rapid and efficient molecular transfer.
Galileo semi-dry electroblotters provide quick and efficient transfer of nucleic acids and proteins from agarose or acrylamide gels. Since only the tranfer "sandwich" of gel, membrane and blotting papers must be kept wet, much less buffer is required than traditional tank transfer systems. Solid plate style electrodes (stainless steel cathode and platinum anode) assure even and complete molecular transfer.

The larger format 2020 system is ideal for transfer of larger gels or simultaneous transfer of multiple smaller gels. System is supplied complete with power leads and a sample pack of our ultra-pure cotton fiber filter paper.
Item#:
ASSEMIELECT2020
Polyolefin, 2.8 mil, Ultra-

These optically clear polyester films are ideally suited for Real Time PCR (qPCR). They feature an inert, ultra-clear, and strong encapsulated silicone adhesive that provides extraordinary performance in real-time PCR applications.

Encapsulated silicone greatly reduces the risk of sample contamination due to exposure to adhesive. Once the film is pressed onto the plate, the raised portion of the well penetrates the encapsulation and a tight seal is formed on just that surface (not over the sample). This silicone seal adheres aggressively and diminishes the possibility of "Edge Effect".

The plasticized backing of this film is scored on both ends to allow for two handed (precise) placement on the plate. Both film and adhesive maintain incredible clarity and are the choice for highly demanding manual and automated processes.

Certified free of RNase, DNase, Pyrogen, and nucleic-acid contamination.

Length

141.27 mm

Length (w/ end tabs removed)

117.5 mm

Width

77.8 mm

Overall Thickness

2.8 mil

Adhesive Thickness

2.0 mil

Material

Polyester

Adhesive

Encapsulated Silicone

Max. Temperature

120 °C

Min. Temperature

-40 °C

Primary Usages

Real Time PCR (qPCR), PCR
Item#:
MID-UC500
Your Price:
1114.00
Each
Storage Film

The polyester films are a great general purpose seal; ideal for sample protection and elimination of well-to-well sample cross contamination. The acrylic adhesive offers a strong bond that seals tightly on polypropylene and polystyrene plates.

This seal is ideally suited for short term storage, Elisa assays, prevention of sample loss, and general lab usage.

The paper backing of this film is scored on both ends to allow for two handed (precise) placement on the plate.

For PCR applications see our standard PCR film or our Real Time PCR film.

For applications where accessing samples through the seal with pipet tips or a probe is required, see the pierceable aluminum (MID-PCR-200) film.

Certified free of RNase, DNase, Pyrogen, and nucleic-acid contamination.

Length

146.05 mm

Length (w/ end tabs removed)

119.05 mm

Width

79.38 mm

Overall Thickness

3.1 mil

Adhesive Thickness

1.1 mil

Material

Polyester

Adhesive

Medical Grade Acrylic

Max. Temperature

120 °C

Min. Temperature

-40 °C

Primary Usages

Elisa, Storage or Sample Cover
Item#:
MID-PCR-TS
Your Price:
195.00
Each
This item is discontinued
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