Bullseye 1Kb DNA Ladder, Logic

• Ready to use
• Contains 16 DNA bands: 100bp-10Kb
• 920ng DNA/ 6 uL/loading
• Easy quantification of DNA fragments
• Stable at room temperature
• Supplied with 6x sample loading buffer

Bullseye 1Kb DNA Ladder, Logic contains 16 DNA bands ranging from 100bp to 10Kb, formulated so that each band contains an amount of DNA that correlates logically to its size, allowing the user to estimate both the size and the quantity of specific fragments at a glance. It is particularly useful for protocols such as probe labeling, DNA sequencing and optimizing insert/vector ratio in ligation reactions, where DNA concentration must be taken into consideration.

Size: 1200 uL
Storage: Store at -20°C.
Concentration: 920 ng/6 uL

Loading Buffer Composition:

• 10mM Tris-HCl
• 1mM EDTA (pH 8.0)
• 0.02% Bromophenol blue
• 0.02% Xylene cyanol
• 5% Glycerol

Usage: Add 6 uL of Bullseye 1Kb DNA Ladder, Logic directly to wells designated for markers, which will yield the amount as indicated in the picture. You may use more than 6 uL of ladder, depending on well size and level of dye used to visualize the bands. Calculate the amount for each band according to your loading volume.

This brand is being discontinued and will only be available while supplies last.

Need a great DNA Ladders at a great price?

Try our PR1MA™ SmartCheck DNA Ladders

100bp DNA Ladder RTU (Ready-to-Use)

• A unique combination of PCR products and a number of proprietary plasmids digested with appropriate restriction enzymes to yield 11 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis.
• The DNA includes fragments ranging from 100-1,500 base pairs. The 500 and 1,500 base pair bands have increased intensity to serve as reference points.
• The approximate mass of DNA in each band is provided (0.5 ug a load) for approximating the mass of DNA in comparably intense samples of similar size.

Source:

• PCR products and double-stranded DNA digested with appropriate restriction enzymes, are phenol and equilibrated to 10nM Tris-HCl (pH 8.0) and 10 mM EDTA.

Range: 100-1,500 bp (DL1); 250-10,000 bp (DL5)
Number of bands: 11 (DL1); 13 (DL5)
Concentration: 100 ug / mL
Package: 50 ug / 500 uL
Recommended Load: 5 uL / well
Contains orange G & xylene cyanol FF as tracking dyes. (DL1)
Contains bromophenol blue & xylene cyanol FF as tracking dyes. (DL5)
Storage:

• Store at 25°C for 6 months
• Store at 4°C for 12 months
• Store at -20°C for 24 months

Bullseye 100bp DNA Ladder

• Ready to use
• Contains 11 DNA bands: 100-1500bp.
• Clearly identifiable 500bp band as reference
• 500ng DNA/6 uL/loading
• Easy to load
• Stable at room temperature
• Supplied with 6x sample loading buffer

Bullseye 100bp DNA Ladder consists of 11 DNA fragments ranging in size from 100-1500 base pairs (bp). 6 uL will yield at least 30 ng DNA in any single band. The intensity of the 500bp band has been increased to serve as a reference for easy identification.

Size: 1200 uL
Storage: Store at -20°C.
Concentration: 500 ng/6 uL

Loading Buffer Composition:

• 10mM Tris-HCl
• 1mM EDTA (pH 8.0)
• 0.02% Bromophenol blue
• 0.02% Xylene cyanol
• 5% Glycerol

Usage: Add at least 6 uL Bullseye 100bp DNA Ladder directly to wells designated for markers. You may need more than 6 uL of ladder, depending on well size and level of intensity needed to visualize the bands.

Please give us a call for a sample.

ENDURO™ Semi Dry Blotter

• Western, Southern, and Northern Blots
• Rapid transfer times
• Uniform head dispersion
• Variable gel thickness
• Minimal buffer volume

The ENDURO™ Semi-Dry Blotter provides great flexibility in a lab since it can be used for all types of blotting: Western, Southern, and Northern. Blotting with the ENDURO™ Semi Dry Blotter typically can be done in 15 to 30 minutes making it a fast and easy way to do all your blotting applications. Set up procedures are easy and economical with little buffer needed. The screw down lid adjusts to varying gel thicknesses and sizes while the platinum coated electrodes provide uniform pressure ensuring even transfers. To provide your lab with total blotting solutions use with our ENDURO™ 250V, 3000mA high current power supply.

Specifications

Semi-Dry Blotting Systems, 10cm x 10cm

PR1MA™ Reverse Transcriptase, RNase H -

PR1MA™ reverse transcriptase (RT) is an RNA-dependent DNA polymerase ideal for use in RT-PCR and first-strand synthesis of complementary DNA (cDNA) for generation of cDNA libraries from single-stranded RNA, DNA, or RNA:DNA hybrids.

• Decreased RNase H activity enables longer cDNA synthesis (>5 kb).
• Lacks 3’ to 5’ exonuclease activity
• Optimal temperature at 42°C
• Temperature range: 40 to 50°C
• Heat inactivation: 70°C for 20 minutes
• Storage temperature: -20°C
• 10X reaction buffer included 

Buffer composition

• 50% glycerol
• 50 mM Tris-HCl
• 50 mM KCl
• 1 mM DTT
• 0.1  mM EDTA
• pH = 7.5

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.

Accuris MyVolt™ Mini Power Supply

• Compact and simple
• Choose constant 100 V or 200 V output
• Two pairs of output terminals

The Accuris MyVolt Mini is a compact electrophoresis power supply ideal for basic, constant voltage applications. Users can select constant 100 V or 200 V output, and the current can reach a maximum of 200 mA.

Two sets of output jacks are available to connect up to 2 gel rigs and these small power supplies can securely be stacked for use with additional gel rigs.

Specifications

Wide Format Gel System 23.5 cm x 14 cm

•     Heavy duty lower buffer chamber
•     Interlock safety lid with attached leads
•     Non-gasketed 23cm wide x 14cm long UV-transparent gel tray with built-in fluorescent ruler and 4 comb slots.
•     Four 1.5mm x 50 tooth combs
•     Opaque well visualization plate.