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PCR/qPCR

MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more.  If you are looking for any of these things, we can match almost all needs to all cyclers.  If you don’t find it easily contact us at tech@midsci.com and we will find it for you. 

96 or 384 we have it.  Non-Skirted, semi-skirted or full skirted, we have it.  0.1, 0.2, or 0.5 mL we have it.  A1, A12, H12, A24, P24 cuts, we have them.  Sybr© Green or probe alternative, we have it.  

Pro Tip: Colored plastics do not have any impact on DNA amplification.  But when setting up qPCR it is often recommended to use white plastics.  This will limit or prevent fluorescence refraction.  Light refraction can minimize sensitivity and consistency. 

Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips.  This will help optimize and reduce variance in the reactions.  Simple test, see the residual liquid left in tips?  This means some of the taq, dNTPs, sample, etc is left in the tip.  PR1MA tips help reduce that significantly and improve your consistency. 

Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate?  Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler.  Call us we have solutions.

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Strips, 1000/cs for

These Rotocycler strips are perfectly designed to perform on Rotor-Gene instruments. Tube strips are packaged separately from cap strips. The frosted extensions on caps not only make them more efficient and secure during handling but also offer a convenient area for labeling. For individual use, tube and cap strips can easily be separated and used as individual units.

Item#:
T319-4N
Your Price:
511.43
Each
Thick Adhesive, Leaves No Residue Behind


Click to shop new and improved eXTReme™ 2 Seal Extra Strong Sealing Film


eXTReme™ Seal Sealing Film with Extra Strong Adhesive Minimizing Evaporation Without Residue

eXTReme™ Seal features an extra-strong thick adhesive to minimize evaporation, yet removes easily without leaving residue on the plate.

Features chamfered corners to eliminate plate overhang for robotics applications and tight storage conditions.

  • Certified DNase- & RNase- free
  • Ideal for use with raised-rim and non-skirted PCR plates
  • Available in a larger cut for flat-top semi-skirted and full-skirted PCR plates: eXTReme™ Seal, Large

Application

  • PCR
  • Robotics
Temperature Range
  • -20°C to +120°C

Helpful Tips

  • Selection of the proper film size will minimize evaporation
  • The film should not overhang or ride up the wall of a raised-rim plate
  • More complete coverage of skirted flat-top plates will reduce evaporation in the outer wells
  • End-tab removal before thermal cycling is highly recommended


Item#:
ASPCRSEALFILM
 
eXTReme Seal Features
Specs eXTReme Seal
Sealing Film Selection Guide by Application
Sealing Film Selection Guide by Attributes
Optically Transparent, Highly Conformable Adhesive

ThermalSeal® RT2™ Optically Transparent, Highly Conformable Adhesive, Polyester Sealing Film

Designed for use with standard 96-well plates where rows must be selectively protected or accessed. Available in a smaller cut for raised-rim and non-skirted PCR plates: ThermalSeal® RT2RR 

  • Certified DNase- & RNase- free
  • Ultra-high optical clarity
  • Minimizes evaporation and cross-contamination
  • Ideal for use with flat-top and hard-shell dual component (e.g. polycarbonate / polypropylene) PCR plates
  • Not recommended for removal after thermal cycling

Application

  • qPCR
Temperature Range
  • -40°C to +120°C

Helpful Tips

  • Selection of the proper film size will minimize evaporation
  • The film should not overhang or ride up the wall of a raised-rim plate
  • More complete coverage of skirted flat-top plates will reduce evaporation in the outer wells
  • End-tab removal before thermal cycling is highly recommended

Item#:
ASQPCRFILM
 
ThermalSeal RT2 Features
Specs ThermalSeal RT2
Sealing film selection guide by application
Sealing film selection guide by attributes

MyView™ Compact UV Transilluminator

  • Uniform Illumination | Four 302 nm Bulbs
  • Adjustable UV Safety Cover | Easy Access to Gels
  • Large Scratch Resistant Viewing | 16.5 x 13.5 cm

The E3100 Compact UV Transilluminator has a surprisingly small footprint (just over 12 x 8 inches) yet provides industry leading performance. It will accommodate one or two mini gels on the 16.5 x 13.5 cm scratch resistant viewing surface.

                                                                   

The integrated UV blocking cover is made from a polymer that will not fluoresce in UV light. This allows clear viewing of DNA bands through the cover yet offers full UV blocking protection. Two friction hinges on the front edge of the cover allow for full adjustment. Position the cover horizontally for general viewing, tilt it at an angle for gel access and band cutting, or open fully to use the SmartDoc™ Imaging Enclosure (sold separately) for gel imaging with a smartphone.

 

The transilluminator’s four 6W bulbs evenly illuminate the viewing surface, and the 302 nm wavelength is ideal for Ethidium Bromide, SmartGlow™, SYBR Green®, Gel Green™ and many other common gel stains.  Spacing of the UV bulbs, coupled with a reflector system and special diffusing filter glass provides exceptional uniformity across the viewing surface. This allows for quantification of DNA and higher quality imaging when used with a camera system.


Specifications
 
Light source 4 x 6W UV Light Bulbs4 x 6W UV Light Bulbs
Safety cover UV blocking polycarbonateUV blocking polycarbonate
Exterior dimensions10 x 8 x 2.75 In. | 25.4 x 20.32 x 6.99 cm
Electrical12VDC (AC power adapter included)
Output wavelengthPeak at 302 nm
Viewing surface6.5 x 5.3 In. | 16.5 x 13.5 cm
Weight 2 lbs. | 0.9 kg.
Warranty2 years
 
 

Item#:
ASUVBENCHTRANS

Double sided water cooled vertical system, 10cm x 10cm


Reflection double sided water cooled vertical system, 10cm x 10cm with or without casting base, two pack each of plain and notched glass, one acrylic blocker plate, (2) 10-well 0.8mm thickness combs, (4) 0.8 mm spacers. Four year warranty.

System Description
 
Run either one or two gels simultaneously on these versatile separation systems. The 10x10cm size of the system also accomodates most pre-cast gels.

Complete double sided vertical system for running one or two gels, in a 10 x 10 cm format. Unique casting bases allows both casting and running of gels without unclamping from the upper buffer chamber, providing for safer and more convenient gel handling. In addition the 1010 device will accommodate most precast gel cassettes on the market.

Water cooling feature is included for protocols that require it. Connections are easily made by attaching standard tubing over the 3/8" barbed in/out ports provided on the device. Water may be provided by tap or other temperature controlled water source. Optional alumina backer plates may be used to facilitate cooling efficiency and uniformity, and are available in the accessories list below.

System Features
 
The Galileo Reflection™ 85-1010 complete system includes these features:

  • Heavy duty upper buffer chamber with cooling port connections.
  • Lower buffer chamber.
  • Interlock safety lid with attached leads.
  • Casting base.
  • Two-pack each of plain & notched glass plates.
  • One acrylic blocker plate (used when running only one gel).
  • Ttwo 10-well, 0.8mm thick combs
  • Four 0.8mm thick side spacers.
  • System Accessories
  • Choose amoung these accessories to customize each system for your specific needs.
  • Alumina cooling plate.
  • For use with cooling systems.
  • This plate can be sandwiched between the gel and the inner buffer chamber to improve overall temperature uniformity.
  • Casting base.
  • For quick, efficient and leak-proof casting.
  • Multiple gradient caster.
Item#:
ASDOUBSIDEWAT10C

Extra Wide Large Format Gel System, 23.5cmW x 40cm

 
Extra wide large format gel system, 23.5cmW x 40cmL gel size with buffer exchange ports, UVT gel tray with fluorescent ruler and 12 tray slots, (2) 25 teeth combs, 1.5 mm thickness and (2) 50 teeth combs, 1.5 mm thickness.
System Description

For long run lengths, maximum resolution, and really large sample capacity, look no further than the Galileo 2340 device. This unit also features the Galileo ExpressCast system for quick tape-less gel pouring.

The 2340 is our maximum capacity system. With a maximum run length of 25cm, the unit can provide very high resolution of complex analytes. Or, fitted with maximum capacity combs in all of the tray's 12 comb slots, the unit can handle up to 600 samples. The 2340 features the Galileo ExpressCast gel casting system for quick, tape-free gel casting and includes a built-in bubble level and leveling screws for convenient level adjustment. You may choose from a wide variety of comb options to customize your order.
System Features

The Galileo 2340 complete system includes these features:

  •     Heavy duty lower buffer chamber
  •     Interlock safety lid with attached leads
  •     End gated 23cm wide x 40cm long ExpressCast™ UV-transparent gel tray with built-in fluorescent ruler and 12 comb slots.
  •     Two 1.5mm x 25 tooth combs
  •     Two 1.5mm x 50 tooth combs
Item#:
ASEXTRAWIDELGFOR23

MaGELin Mini Protein System

  • For 10 cm x 10 cm cast-your-own polyacrylamide gels
  • Comes with dual casting system
  • 3 sets of glass plates and 2 sets of 0.8 mm thickness spacers.
  • Buffer tank, dual gel capture, vented lid and power cords included

The IB94000 MaGELin Mini protein system comes complete with everything you need to pour 10 cm x 10 cm cast-your-own gels. This highly versatile system will also accommodate the IB95000 Western Transfer module so when you’re not running gels the unit can be used as a dedicated Western transfer system.
 

The IB94000 comes complete with:

  • Dual gel capture device for running two gels simultaneously.
  • Dual gel casting system that allows casting outside of the running unit.
  • Three sets of glass plates for pouring and running gels
  • Two sets of special 0.8 mm side spacers to seal the gel sandwich during pouring
  • Buffer tank
  • Vented lid
  • Power cords.
  • Two 0.8 mm x 12-tooth combs.

The IB94000 utilizes a unique spacer and casting system to make it easy and fast to cast your own gels. The special side spacers are made specifically to accommodate the thickness of each glass slide that comes with the unit. These side spacers will produce a 0.8 mm thick gel. Once the glass plates have been assembled using the side spacers, they can now be inserted into the gel casting unit. This unit accommodates two sets of glass plates simultaneously. The bottom of the gel casting unit contains a very soft gel-like gasket that will seal the bottom of the gel sandwich. Once the gel sandwich is placed inside the casting unit, place the lid of the casting unit on top and press gently to fasten using the included thumb screws. Once fastened the lid will provide the correct amount of down pressure on the gel sandwich to seal the bottom and hold them in place. The polyacrylamide solution can now be introduced through the opening in the casting fixture lid. 

Item#:
ASMAGELINMINI
White Light, 21x26cm, Single

Uniform white light diffusion for viewing negative and film positives. X-ray film, autoradiographs, microtiter plates, and stained gels can be viewed on the white light transmitting glass surface. This surface, permanently double-safety sealed into the unit for durability, provides a scratch and chemical resistant work area which can be cleaned easily. Durable transilluminator housing is scratch resistant, epoxy powder paint finish for easy cleaning. 2 year warranty

ModelOrder NoDescriptionFilter SizeDimensions
TW-26 95-0208-01White Light Transilluminator, 115V 21 x 26cm9.5"D x 13.25"W x 4.25"H
TW-4395-0214-01White Light Transilluminator, 115V 36 x 43cm19.13"D x 16"W x 4.25"H

Safety Certifications: ETL, ETL/c on all 115V models.
Item#:
95-0208-01
Your Price:
1149.86
Each

Extra Wide Format Gel System, 23.5cmW x 25cmL


 ExpressCast extra wide format gel gystem for 23.5cmW x 25cmL gel size. Includes UVT gel tray with fluorescent rulers and 14 comb slots, (2) 25 tooth combs, 1.5 mm thickness, and (2) 50 tooth combs, 1.5 mm thickness and gasketed end-gates.
System Description

Need long run lengths for maximum resolution, and large sample capacity too? Look at the features of the Galileo 2325 unit and we think you will find just what you need.

The 2325 is a full sized large capacity system. With a maximum run length of 25cm, the unit can provide high resolution of complex analytes. Or, fitted for maximum capacity, the unit can handle up to 500 samples. The 2325 features the Galileo ExpressCast gel casting system for quick, tape-free gel casting and includes a built-in bubble level and leveling screws for convenient level adjustment. You may choose from a wide variety of comb options to customize your order.
System Features

The Galileo 2325 complete system includes these features:

  •     Heavy duty lower buffer chamber
  •     Interlock safety lid with attached leads
  •     End gated 23cm wide x 25cm long ExpressCast™ UV-transparent gel tray with built-in fluorescent ruler and 14 comb slots.
  •     Two 1.5mm x 25 tooth combs
  •     Two 1.5mm x 50 tooth combs
Item#:
ASEXTRAWIDEFOR23

Accuris™ SmartBlue™ Transilluminators

 

Accuris SmartBlue Transilluminators have been developed for viewing DNA gels stained with new, safe fluorescent dyes.  These new dyes, such as SmartGlow, SYBR Green, Gel Green, have an excitation wavelength of 465-470nm (visible blue light), and are safer to use than Ethidium Bromide.

  • Blue light, safe for nucleic acids
  • Uniform illumination (UI Technology)
  • Optimal wavelength (465nm) for common safe stains
  • Adjustable position, amber filter covers
  • Optional amber viewing glasses: E4000-VG1

Safer for samples, safer for you: Accuris SmartBlue Transilluminators are the perfect partner for the most common safe DNA stains. These instruments emit blue light at a wavelength of 465nm, perfect for excitation of most green fluorophors, including our Accuris’ SmartGlow™, SYBR Green, SYBR Safe, SYBR Gold, GelStar, GelGreen, to name a few.  SmartBlue transilluminators can also work for excitation of EtBr (though fluorescence is not as bright as with UV), see our Technical Bulletin with sample images.   Exposing DNA to blue wavelength does not cause molecular damage, and results in a much higher downstream cloning efficiency as compared to even short UV light exposures.  In addition, the visible blue light is not damaging to the user’s skin and is less harmful to the eyes.*

Adjustable filter cover: Both the E4101 and SB4500 transilluminators come with emission filter covers. These amber covers, which rest above the viewing surface and gel, filter out the blue light, allowing the fluorescing DNA bands to be seen clearly.  The covers can be set to a flat position for general gel viewing or can be set at an angle to provide easy access to the gel for band excision.  The SB4500 has a two-position filter cover, and the E4101 features a hinged filter cover.

 

Optional amber viewing glasses: Our E4000-VG1 SmartBlue Plus Viewing Glasses offer efficient blocking of the blue light wavelength from the transilluminators, allowing clear visualization of the fluorescing DNA bands without the filter cover in place.  These convenient glasses are recommended for users that are frequently excising DNA bands from gels.  SmartBlue Plus glasses also block 100% of UV wavelength so they can also be used for viewing gels on a UV transilluminator.

 

UI technology, bright and clear images: The bright LED light plates and filter layer provides an extremely evenly lit viewing surface. Even in ambient light, bands of DNA can clearly be seen in stained gels. Images obtained using the SmartBlue Transilluminator are brighter and more balanced than those obtained using epi-illuminators which light from the sides.


Reliable, durable, and long-lasting: An array of super bright LEDs with a long, 30,000 hour service life provide the light source for the SmartBlue Transilluminators. Unlike those in a UV transilluminator, the filters in the SmartBlue systems will not solarize and degrade in performance over time. Gel bands can be excised directly on the scratch resistant, glass viewing surfaces. To save energy, the power switch includes an automatic 5 minute shutoff. SmartBlue Transilluminators are covered by a 2 year warranty.

 

* The SmartBlue products use high intensity LED lights.  Although the visible blue wavelength is significantly safer than UV light, it is recommended to not stare directly at the viewing surface without the amber cover in place. 

 


Specifications 

Light SourceHigh Intensity Blue LEDs
Emission Filter CoverAmber
WavelengthPeak at 465 nm
E4000 Viewing Surface17 x 12 cm
E4000 Ext. Dimensions30.5 x 21.5 x 5 cm
E4101 Viewing Surface10.5 x 10.5 cm
E4101 Ext. Dimensions17 x 12 x 5 cm
Electrical100 to  240V, 50-60Hz
E4000 Weight1.2kg / 2.6lbs
E4101 Weight0.34kg / 0.75lbs
Warranty2 years
 
 

Item#:
ASSMARTBLUELGT


PR1MA™ Reverse Transcriptase

 

PR1MA™ reverse transcriptase (RT) is an RNA-dependent DNA polymerase that can be used for complementary DNA (cDNA) synthesis from an RNA template and is ideal for use in molecular amplification assays. PR1MA™ RT is a robust enzyme that works in a broad range of temperatures (40 - 72°C) and has RNase H activity.

  • Optimal temperature: 55°C
  • Heat inactivation: 75°C for 20 minutes
  • Glycerol-free buffer available
  • Storage temperature: -20°C (standard buffer)
  • 10X Isothermal buffer included

Standard Buffer Composition

  • 50% glycerol
  • 10 mM Tris-HCl
  • 100 mM KCl
  • 1 mM DTT
  • 0.1  mM EDTA
  • pH = 7.5

Important note: Please be sure to use the buffer provided with this product to ensure optimal results.

 

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.   
 

Item#:
ASPRIMART
 
Safety Sheet for PR1MA Reverse Transcriptase
Specs for PR1MA Reverse Transcriptase

Double sided water cooled vertical system, 20cmW x 10cm


The Galileo Bioscience Reflection 2010 is a wide, high capacity gel electrophoresis separation system that allows users to run up to two protein gels simultaneously on a 20 cm wide x 10 cm tall format.  The large gel format makes this unit ideal for users who have a large number of protein samples to run simultaneously.  Galileo's unique casting base allows users to both cast and run gels without ever having to unclamp the upper buffer chamber.  This makes handling gels safer and more convenient, especially for those who may be prone to ripping their gels between the two activities. 

The 2010 vertical gel box also includes a water cooling feature for electrophoresis protocols that require lower temperature.  This water can be provided by the tap or other temperature controlled source and is easily connected by attaching standard tubing over the 3/8" barbed in/out ports on the device.  Users may also facilitate cooling efficiency and uniformity using Galileo's optional alumina backer plates (see accessories below).

System Features

Each Reflection 85-2010-V vertical system includes the following features:

  • Heavy duty upper buffer chamber with cooling port connections
  • Lower buffer chamber
  • Interlock safety lid with attached leads
  • Casting base
  • Four-pack each of plain and notched glass plates
  • One acrylic blocker plate (used when only one gel is being run)
  • Eight 0.8mm thick side spacers
  • Four combs of your choice
  • System Accessories

Alumina cooling plate for use with cooling systems. This plate can be sandwiched between the gel and the inner buffer chamber to improve overall temperature uniformity.
 
Casting base for quick, efficient and leak-proof gel casting.
Item#:
ASDOUBLESIDEWATVER20
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