S2K Commerce - Products Dropdown
ActionsS2K Commerce - Shopping Cart
ActionsS2K Commerce - Order Entry
ActionsPCR/qPCR
MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more. If you are looking for any of these things, we can match almost all needs to all cyclers. If you don’t find it easily contact us at tech@midsci.com and we will find it for you.
96 or 384 we have it. Non-Skirted, semi-skirted or full skirted, we have it. 0.1, 0.2, or 0.5 mL we have it. A1, A12, H12, A24, P24 cuts, we have them. Sybr© Green or probe alternative, we have it.
Pro Tip: Colored plastics do not have any impact on DNA amplification. But when setting up qPCR it is often recommended to use white plastics. This will limit or prevent fluorescence refraction. Light refraction can minimize sensitivity and consistency.
Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips. This will help optimize and reduce variance in the reactions. Simple test, see the residual liquid left in tips? This means some of the taq, dNTPs, sample, etc is left in the tip. PR1MA tips help reduce that significantly and improve your consistency.
Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate? Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler. Call us we have solutions.
Extra Wide Large Format Gel System, 23.5cmW x 40cm
Extra wide large format gel system, 23.5cmW x 40cmL gel size with buffer exchange ports, UVT gel tray with fluorescent ruler and 12 tray slots, (2) 25 teeth combs, 1.5 mm thickness and (2) 50 teeth combs, 1.5 mm thickness.
System Description
For long run lengths, maximum resolution, and really large sample capacity, look no further than the Galileo 2340 device. This unit also features the Galileo ExpressCast system for quick tape-less gel pouring.
The 2340 is our maximum capacity system. With a maximum run length of 25cm, the unit can provide very high resolution of complex analytes. Or, fitted with maximum capacity combs in all of the tray's 12 comb slots, the unit can handle up to 600 samples. The 2340 features the Galileo ExpressCast gel casting system for quick, tape-free gel casting and includes a built-in bubble level and leveling screws for convenient level adjustment. You may choose from a wide variety of comb options to customize your order.
System Features
The Galileo 2340 complete system includes these features:
- Heavy duty lower buffer chamber
- Interlock safety lid with attached leads
- End gated 23cm wide x 40cm long ExpressCast™ UV-transparent gel tray with built-in fluorescent ruler and 12 comb slots.
- Two 1.5mm x 25 tooth combs
- Two 1.5mm x 50 tooth combs
MaGELin Mini Protein System
- For 10 cm x 10 cm cast-your-own polyacrylamide gels
- Comes with dual casting system
- 3 sets of glass plates and 2 sets of 0.8 mm thickness spacers.
- Buffer tank, dual gel capture, vented lid and power cords included
The IB94000 MaGELin Mini protein system comes complete with everything you need to pour 10 cm x 10 cm cast-your-own gels. This highly versatile system will also accommodate the IB95000 Western Transfer module so when you’re not running gels the unit can be used as a dedicated Western transfer system.
The IB94000 comes complete with:
- Dual gel capture device for running two gels simultaneously.
- Dual gel casting system that allows casting outside of the running unit.
- Three sets of glass plates for pouring and running gels
- Two sets of special 0.8 mm side spacers to seal the gel sandwich during pouring
- Buffer tank
- Vented lid
- Power cords.
- Two 0.8 mm x 12-tooth combs.
The IB94000 utilizes a unique spacer and casting system to make it easy and fast to cast your own gels. The special side spacers are made specifically to accommodate the thickness of each glass slide that comes with the unit. These side spacers will produce a 0.8 mm thick gel. Once the glass plates have been assembled using the side spacers, they can now be inserted into the gel casting unit. This unit accommodates two sets of glass plates simultaneously. The bottom of the gel casting unit contains a very soft gel-like gasket that will seal the bottom of the gel sandwich. Once the gel sandwich is placed inside the casting unit, place the lid of the casting unit on top and press gently to fasten using the included thumb screws. Once fastened the lid will provide the correct amount of down pressure on the gel sandwich to seal the bottom and hold them in place. The polyacrylamide solution can now be introduced through the opening in the casting fixture lid.
Uniform white light diffusion for viewing negative and film positives. X-ray film, autoradiographs, microtiter plates, and stained gels can be viewed on the white light transmitting glass surface. This surface, permanently double-safety sealed into the unit for durability, provides a scratch and chemical resistant work area which can be cleaned easily. Durable transilluminator housing is scratch resistant, epoxy powder paint finish for easy cleaning. 2 year warranty
Model | Order No | Description | Filter Size | Dimensions |
TW-26 | 95-0208-01 | White Light Transilluminator, 115V | 21 x 26cm | 9.5"D x 13.25"W x 4.25"H |
TW-43 | 95-0214-01 | White Light Transilluminator, 115V | 36 x 43cm | 19.13"D x 16"W x 4.25"H |
Safety Certifications: ETL, ETL/c on all 115V models.
Extra Wide Format Gel System, 23.5cmW x 25cmL
ExpressCast extra wide format gel gystem for 23.5cmW x 25cmL gel size. Includes UVT gel tray with fluorescent rulers and 14 comb slots, (2) 25 tooth combs, 1.5 mm thickness, and (2) 50 tooth combs, 1.5 mm thickness and gasketed end-gates.
System Description
Need long run lengths for maximum resolution, and large sample capacity too? Look at the features of the Galileo 2325 unit and we think you will find just what you need.
The 2325 is a full sized large capacity system. With a maximum run length of 25cm, the unit can provide high resolution of complex analytes. Or, fitted for maximum capacity, the unit can handle up to 500 samples. The 2325 features the Galileo ExpressCast gel casting system for quick, tape-free gel casting and includes a built-in bubble level and leveling screws for convenient level adjustment. You may choose from a wide variety of comb options to customize your order.
System Features
The Galileo 2325 complete system includes these features:
- Heavy duty lower buffer chamber
- Interlock safety lid with attached leads
- End gated 23cm wide x 25cm long ExpressCast™ UV-transparent gel tray with built-in fluorescent ruler and 14 comb slots.
- Two 1.5mm x 25 tooth combs
- Two 1.5mm x 50 tooth combs
The SmartBlue™ Blue Light Transilluminator
The SmartBlue™ Blue Light Transilluminator is the smart alternative to UV transillumination. The uniform 465 nm bottom lighting illumination is perfect for excitation of most green fluorophores without damaging the DNA, thus resulting in a much higher downstream cloning efficiency. Furthermore, the two position filter cover position allows for easy access to the gel for band excision. Without the need for the use of DNA damaging UV light, you don't have to worry about any damage to the skin and eyes.
The LEDS are long lasting (with a 30,000 hour service life) providing a durable and efficient illumination option. Gels can be excised directly on the unit with the scratch resistant surface. The unit contains an automatic 5 minute timed shut off for energy saving as well. The cover can be placed flush with the unit, at a 60 degree angle, or completely removed for use with the SmartDoc Imager (E5000-SD).
Pair with DNA Safe Stain (C138) for the ultimate viewing effectiveness!
A 230V is available upon request (SB4500-E).
Specifications
Light Source: | High Intensity Blue LEDs |
Emission Filter Cover: | Amber |
Wavelength: | Peak at 465 nm |
E4000 Viewing Surface: | 17 x 12 cm |
E4000 Ext. Dimensions | 30.5 x 21.5 x 5 cm |
E4100 Viewing Surface | 10.5 x 10.5 cm |
E4100 Ext. Dimensions | 17 x 12 x 5 cm |
Electrical: | 100 to 240V, 50-60Hz |
Weight: | 1.2kg / 2.6lbs |
Warranty: | 2 years |
SmartBlue™ Mini Blue Light Transilluminator
Safer for samples, safer for you: Accuris SmartBlue™ Mini Blue Light Transilluminator is the perfect partner for most common safe DNA stains. It emits blue light at a wavelength of 465nm, perfect for excitation of most green fluorophors, including our Accuris’ SmartGlow™, SYBR Green, SYBR Safe, SYBR Gold, GelStar, GelGreen, to name a few. The SmartBlue™ Mini also works for excitation of EtBr (though fluorescence is not as bright as with UV), see our Technical Bulletin with sample images. Exposing DNA to blue wavelength does not cause molecular damage, and results in a much higher downstream cloning efficiency as compared to even short UV light exposures. In addition, the visible blue light is not damaging to the user’s skin and is less harmful to the eyes.*
Two position filter cover: The E4100 transilluminator comes with an emission filter cover. These amber covers, which rest above the viewing surface and gel, filter out the blue light, allowing the fluorescing DNA bands to be seen clearly. The covers are designed to fit flat for general gel viewing or to be placed at an angle to provide easy access to the gel for band excision. For documentation, the filter cover is easily removed completely. UI technology, bright and clear images: The bright LED light plates and filter layer provides an extremely evenly lit viewing surface. Even in ambient light, bands of DNA can clearly be seen in stained gels. Images obtained using the SmartBlue™ Mini Transilluminator are brighter and more balanced than those obtained using epi-illuminators which light from the sides.
Reliable, durable and long lasting: An array of super bright LEDs with a long, 30,000 hour service life provide the light source for the SmartBlue™ Mini Transilluminator. Unlike those in a UV transilluminator, the filters in the SmartBlue™ Min system will not solarize and degrade in performance over time. Gel bands can be excised directly on the scratch resistant, glass viewing surfaces. To save energy, the power switch includes an automatic 5 minute shutoff. SmartBlue™ Mini Transilluminators are covered by a 2 year warranty.
* The SmartBlue™ products use high intensity LED lights. Although the visible blue wavelength is significantly safer than UV light, it is recommended to not stare directly at the viewing surface without the amber cover in place.
Specifications
Light Source |
High
Intensity LEDs |
Filter Cover |
Amber,
for filtering of blue light |
Exterior Dimensions |
6.7 x
4.7 x 1.9 in | 17 x 12 x 5 cm |
Electrical |
100-240
V, 50/60 Hz |
Output Wavelength |
Peak
at 465 nm |
Viewing Surface |
3.9 x
4.1 in | 10 x 10.5 cm |
Weight |
0.2
kg | 0.5 lb |
Warranty |
2
years |
Optional SmartBlue™ Plus Viewing Glasses
Accuris SmartBlue™ Plus Viewing Glasses offer exceptional protection for researchers working with bright blue LED and UV light illuminators. The polycarbonate filter lenses are shatter proof, scratch resistant and block light in the UV (280 nm) to blue (490 nm) wavelengths. In addition, they are compliant with ANSI Z87.1.
These glasses are ideal for general protection when working with hazardous wavelengths and for precision DNA band cutting on a UV or blue light transilluminator. While blocking hazardous UV and blue light, the glasses allow clear visualization of DNA bands stained with Ethidium Bromide, SmartGlow™, SYBR Green and other fluorescent dyes.
*Always wear appropriate UV protection for eyes and exposed skin when working with UV light sources
Double sided water cooled vertical system, 20cmW x 10cm
- Heavy duty upper buffer chamber with cooling port connections
- Lower buffer chamber
- Interlock safety lid with attached leads
- Casting base
- Four-pack each of plain and notched glass plates
- One acrylic blocker plate (used when only one gel is being run)
- Eight 0.8mm thick side spacers
- Four combs of your choice
- System Accessories
- Increased ultraviolet output and intensity
- No light flicker
- Wider intensity range with variable selection (select models)
- Fast lamp start up
- Universal power supply
- Reduced electrical consumption
- Longer tube life
- Lighter weight
- Improved gel visualization and gel documentation
- 2 year warranty
Visi-Blue Transilluminators are designed for use with GFP stains. As compared to UV, the blue light will transmit through almost any transparent (plastic, glass, etc.) laboratory equipment. This is essential when monitoring and detecting DNA migration and protein expression in real time.
- Two models are available with surface areas as follows: 21x26cm or 20x40cm
- Select from models with 8-watts or 25-watts
- Amber cover (included) blocks blue light transmission and allows visualization of media above 500nm
Several specially designed converter plates convert a transilluminator's 302nm ultraviolet radiation .
- To white light for viewing protein gels, coomassie blue stained or silver stained media. The Converter Plate's uniquely phosphored glass assembly (patent pending) converts the UV radiation via a white diffuser
- Convert UV:
- To 460-470nm with the Visi-BlueT plate for viewing GFP stains
- To 365nm UV with the UV/UV plate for preparation and gel excision
- The scratch-resistant glass is mounted in a metal housing for durability
- Rubber seal stops the plate from sliding
- Handles are situated on two sides of the plate for easy handling
- Several standard plate sizes are available; custom sizes are also available
Contact your rep or email tech@midsci.com for more information.
The ENDURO Mini is a great saver of space and money. This power supply can handle up to 2 gel boxes at atime and is great for horizontal DNA gels and mini-vertical protein gels. These are small in size but not performance. The ergonomic design of the ENDURO Mini allows them to be stacked. However, to minimize cabling, up to three power supplies can be placed within the PowerStock allowing optimal air circulation while only utilizing one power outlet at the bench. These units are full featured with 300V, 400mA, 60W and built in timer. They can run at either constant voltage or constant current and have automatic cross-over as well as no-load detection.
Output voltage/increments |
10-300V/ 1V |
Output current/increments |
10-400mA/1mA |
Output power |
60W |
Operating constant modes |
Constant voltage or constant current |
Timer |
1-999 minutes with alarm, or continuous |
Dimensions (WXDXH) |
140 X 191 X 84 mm |
Weight |
1 kg |
Input voltage |
100-240V |
Contact your rep or email tech@midsci.com for more information.
The ENDURO Mini is a great saver of space and money. This power supply can handle up to 2 gel boxes at atime and is great for horizontal DNA gels and mini-vertical protein gels. These are small in size but not performance. The ergonomic design of the ENDURO Mini allows them to be stacked. However, to minimize cabling, up to three power supplies can be placed within the PowerStock allowing optimal air circulation while only utilizing one power outlet at the bench. These units are full featured with 300V, 400mA, 60W and built in timer. They can run at either constant voltage or constant current and have automatic cross-over as well as no-load detection.
Output voltage/increments |
10-300V/ 1V |
Output current/increments |
10-400mA/1mA |
Output power |
60W |
Operating constant modes |
Constant voltage or constant current |
Timer |
1-999 minutes with alarm, or continuous |
Dimensions (WXDXH) |
140 X 191 X 84 mm |
Weight |
1 kg |
Input voltage |
100-240V |
Need a great (q)PCR Regents at a great price? Try PR1MA! Shop Green, Gold, Probe, or One Step.
New and Improved! Validated for SNP Genotyping assays, Gene expression assays, Microarray validation and High throughput screening!
Bullseye EvaGreen qPCR MasterMix is designed for quantitative/real-time analysis. The components of Bullseye EvaGreen qPCR MasterMix has been developed for superb performance in sensitivity, signal-to-noise ratio, and complete elimination of primer dimers. The chemically modified Hot-Start Taq polymerase, included in our Mastermix, significantly reduces non-specific PCR amplification observed with regular Taq polymerase. Every lot is tested on different real-time PCR machines listed below.
- Instrument-specific & pre-optimized
- Validated applications
- Reproducible results
- Highly competitive pricing
For laboratory research only. Not for clinical applications.
For technical questions, phone the MIDSCI helpline at 800-227-9997
These compact power supplies cover a wide range of requirements for electrophoretic separations. The SH-500 is ideal for DNA or RNA submarine gels, PAGE & SDS-PAGE, multiple horizontal gels and mini blotting applications. It features a built-in timer, constant voltage or constant current, and a unique Gel Saver mode (1mA output to prevent diffusion after the timer expires). The easy-to-use SH-3000 offers constant voltage, current and power, making it perfect for DNA sequencing systems and SDS-PAGE applications. 4 year warranty
Model: | SH-3000 |
Voltage: | 10-3000V, 10V steps |
Max. Current: | 300mA, 1mA steps |
Power: | 300 watts |
Fault Detection: | Stop & Audible Alarm |
Operating Temperature: | 0-40°C |
Dimensions (W x D x H): | 27 x 28 x 11cm |
Weight: | 4kg |