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PCR/qPCR

MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more.  If you are looking for any of these things, we can match almost all needs to all cyclers.  If you don’t find it easily contact us at tech@midsci.com and we will find it for you. 

96 or 384 we have it.  Non-Skirted, semi-skirted or full skirted, we have it.  0.1, 0.2, or 0.5 mL we have it.  A1, A12, H12, A24, P24 cuts, we have them.  Sybr© Green or probe alternative, we have it.  

Pro Tip: Colored plastics do not have any impact on DNA amplification.  But when setting up qPCR it is often recommended to use white plastics.  This will limit or prevent fluorescence refraction.  Light refraction can minimize sensitivity and consistency. 

Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips.  This will help optimize and reduce variance in the reactions.  Simple test, see the residual liquid left in tips?  This means some of the taq, dNTPs, sample, etc is left in the tip.  PR1MA tips help reduce that significantly and improve your consistency. 

Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate?  Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler.  Call us we have solutions.

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Item#:
ASADSEAL1P4
Designed to Facilitate Air and CO2 Exchange

AeraSeal™ Sealing Film Designed to Facilitate Air and CO2 Exchange

AeraSeal™ features a hydrophobic, gas permeable membrane with a medical grade, non-cytotoxic adhesive. The porous membrane consists of layers of heat welded rayon fibers.
  • Pore size from 10 µm to 50 µm
  • Moisture vapor transmission rate = 4,200 g/m²/24 hr
  • Easy-to-pierce with serological pipets and pipet tips for direct sample recovery
  • Available in roll format for automation: AeraSeal™ Roll-Seal
Applications
  • Cell culture
  • Tissue culture
  • Bacterial cultures
Temperature Range
  • -20°C to +80°C

Item#:
ASFILMCULTURES
 
AeraSeal Features
Specs AeraSeal
Sealing film selection guide by application
Sealing film selection guide by attributes
For Fast and Efficient Microplate Sealing

SealPlate® SealMate™ Rolls for Fast and Efficient Microplate Sealing

The SealMate SealPlate Starter Kit features a re-usable dispenser and two rolls of perforated film.
 
The dispenser facilitates the quick and easy dispensing of film from a roll allowing microplates to be sealed three times faster than using sheeted films. Cost-effective replacement rolls are sold separately. Ideal for use under the hood; the SealMate dispenser minimizes film contamination.

  • Environmentally friendly; no paper liner to remove and discard; utilizes approximately 72% less material than SealPlate sheeted film
  • Each sheet is separated by an adhesive-free, perforated, color-coded green band
  • The smooth lower surface of the dispenser can be used to seal the film to the plate
  • Dispenser protects unused seals from contamination
  • Lower support prop prevents dispensing bill from contacting surfaces to prevent contamination
  • Replacement rolls can be easily installed by releasing the locking clasps on the dispenser
Applications
  • General laboratory use
  • Short-term storage
  • Incubation
  • ELISA
Temperature Range
  • -40°C to +120°C

Item#:
ASSEALMATEROLLS
UV Blocking, 3x6in.

Eye and face protection are essential for anyone working with ultraviolet light sources. UV radiation can cause delayed reactions of sunburn to unprotected eyes and skin. Choose from three styles of comfortable eye/face protection: spectacles, goggles, or faceshield. Spectacles are made of impact resistant polycarbonate. The top and side shield design provide full UV protection for the eyes. Goggles are constructed of specially-formulated plastic giving optimum viewing contrast and lessened eye fatigue. The lightweight design and flexible rubber trim of the goggles fit comfortably on the face, even over prescription glasses. Air vents are located on each side of the goggles to allow air flow while blocking UV. The faceshield covers the face and neck area. 

Eyewear complies with ANSI-Z87.1 specifications for safety eyewear and NIOSH Document HSM-83-1109. Criteria for a Recommended Standard - Occupational Exposure to Ultraviolet Radiation.

Item#:
98-0002-02
Your Price:
37.16
Each
UV Blocking, 17x8.25in.

Eye and face protection are essential for anyone working with ultraviolet light sources. UV radiation can cause delayed reactions of sunburn to unprotected eyes and skin. Choose from three styles of comfortable eye/face protection: spectacles, goggles, or faceshield. Spectacles are made of impact resistant polycarbonate. The top and side shield design provide full UV protection for the eyes. Goggles are constructed of specially-formulated plastic giving optimum viewing contrast and lessened eye fatigue. The lightweight design and flexible rubber trim of the goggles fit comfortably on the face, even over prescription glasses. Air vents are located on each side of the goggles to allow air flow while blocking UV. The faceshield covers the face and neck area. 

Eyewear complies with ANSI-Z87.1 specifications for safety eyewear and NIOSH Document HSM-83-1109. Criteria for a Recommended Standard - Occupational Exposure to Ultraviolet Radiation.

Item#:
98-0002-04
Your Price:
118.16
Each
UV Blocking, 2x6in.

Eye and face protection are essential for anyone working with ultraviolet light sources. UV radiation can cause delayed reactions of sunburn to unprotected eyes and skin. Choose from three styles of comfortable eye/face protection: spectacles, goggles, or faceshield. Spectacles are made of impact resistant polycarbonate. The top and side shield design provide full UV protection for the eyes. Goggles are constructed of specially-formulated plastic giving optimum viewing contrast and lessened eye fatigue. The lightweight design and flexible rubber trim of the goggles fit comfortably on the face, even over prescription glasses. Air vents are located on each side of the goggles to allow air flow while blocking UV. The faceshield covers the face and neck area. 

Eyewear complies with ANSI-Z87.1 specifications for safety eyewear and NIOSH Document HSM-83-1109. Criteria for a Recommended Standard - Occupational Exposure to Ultraviolet Radiation.

Item#:
98-0002-01
Your Price:
39.82
Each

IBI DEPC-Treated, Nuclease-Free Water is ideally suited for all applications in molecular biology lab including PCR, RT-PCR, restriction enzyme
assays, modifying enzyme assays, transfection, cloning, transformation, and specifically for all RNA assays.


IBI DEPC-Treated Water is manufactured under stringent conditions. The purification process for this product includes continuous
deionization, reverse osmosis, UV-treatment, 0.1µm filtration, followed by steam sterilization in an autoclave. The product is re-autoclaved
to ensure the breakdown of DEPC and release of carbon dioxide that may cause the pH to shift.


IB42200

DEPC Treated Water, nuclease free, 125mL

IB42201

DEPC Treated Water, nuclease free, 24 x 125mL

IB42202

DEPC Treated Water, nuclease free, 48 x 125 mL

IB42210

DEPC Treated Water, nuclease free, 500 mL

IB42211

DEPC Treated Water, nuclease free, 10 x 500 mL

IB42212

DEPC Treated Water, nuclease free, 20 x 500 mL

IB42220

DEPC Treated Water, nuclease free, 1L

IB42221

DEPC Treated Water, nuclease free, 6 x 1L

IB42222

DEPC Treated Water, nuclease free, 12 x 1L

IB42230

DEPC Treated Water, nuclease free, 2L

IB42231

DEPC Treated Water, nuclease free, 6 x 2L

IB42240

DEPC Treated Water, nuclease free, 10L
Item#:
ASPCRWATER1

Adhesive Sealing Film, Polyester, 3.1 mil


The polyester films are a great general purpose seal; ideal for sample protection and elimination of well-to-well sample cross contamination. The acrylic adhesive offers a strong bond that seals tightly on polypropylene and polystyrene plates.

This seal is ideally suited for short term storage, Elisa assays, prevention of sample loss, and general lab usage.

The paper backing of this film is scored on both ends to allow for two handed (precise) placement on the plate.

For PCR applications see our standard PCR film or our Real Time PCR film.

For applications where accessing samples through the seal with pipet tips or a probe is required, see the pierceable aluminum (MID-PCR-200) film.

Certified free of RNase, DNase, Pyrogen, and nucleic-acid contamination. 


Specifications

Length146.05 mm
Length (w/ end tabs removed)119.05 mm
Width79.38 mm
Overall Thickness3.1 mil
Adhesive Thickness1.1 mil
MaterialPolyester
AdhesiveMedical Grade Acrylic
Max. Temperature120 °C
Min. Temperature-40 °C
Primary UsagesElisa, Storage or Sample Cover
Item#:
ASADHESIVEPOLSTER31
2X Protein Loading Dye
    • Tracks the migration progression of your sample during polyacrylamide electrophoresis
    • Loading dye migrates independently of the samples, making it easier to estimate the migration of proteins
    • DNase/RNase/Protease free
Item#:
IB01190
Your Price:
33.54
Each
This item is discontinued
Item#:
ASADSEALPOLY
For More Secure Seal to Minimize Evaporation

General Purpose Sealing Film For a More Secure Seal to Minimize Evaporation

General Purpose polypropylene films offer superior barrier properties to minimize evaporation and prevent well-to-well contamination. Ideal for use with polypropylene plates.
  • Certified DNase- & RNase- free
  • Features good chemical resistance
  • Provides a very secure seal to minimize evaporation, but is slightly harder to remove than SealPlate films
  • Less rigid polypropylene film reduces aerosols upon removal
  • Specially formulated adhesive minimizes plate residue
  • Can be used as a temporary seal while awaiting further processing
Applications
  • General laboratory use
  • Short-term storage
  • Incubation
  • ELISA
Temperature Range
  • -40°C to +120°C
Item#:
ASGENPURSEAL
 
General Purpose Sealing Film Features
Specs General Purpose Sealing Film
Sealing film selection guide by application
Sealing film selection guide by attributes
Bulk, 250/pkg
  • Safely excise bands from gels
  • Avoid cross contamination
  • One-handed operation for quick and accurate gel cutting
  • Safer than using razor blades and won't scratch transilluminators

Cut gel with pipet tip on the end of a standard 1000µl pipettor. Gel piece is suspended in tip when pipettor is lifted from the gel. Expel the gel piece by pushing the pushbutton on the pipettor. Tip is ejected in normally using the ejector button.

Item#:
TGL-1165
Your Price:
89.86
Each
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