PCR/qPCR

MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more.  If you are looking for any of these things, we can match almost all needs to all cyclers.  If you don’t find it easily contact us at tech@midsci.com and we will find it for you. 

96 or 384 we have it.  Non-Skirted, semi-skirted or full skirted, we have it.  0.1, 0.2, or 0.5 mL we have it.  A1, A12, H12, A24, P24 cuts, we have them.  Sybr© Green or probe alternative, we have it.  

Pro Tip: Colored plastics do not have any impact on DNA amplification.  But when setting up qPCR it is often recommended to use white plastics.  This will limit or prevent fluorescence refraction.  Light refraction can minimize sensitivity and consistency. 

Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips.  This will help optimize and reduce variance in the reactions.  Simple test, see the residual liquid left in tips?  This means some of the taq, dNTPs, sample, etc is left in the tip.  PR1MA tips help reduce that significantly and improve your consistency. 

Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate?  Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler.  Call us we have solutions.

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This brand is being discontinued and will only be available while supplies last.
 
Need a great DNA Safe Stain at a great price? Try PR1MA!  Click here to order.

 

  • Safest DNA stain by far
  • Replaces EthBr in agarose gel electrophoresis
  • Detection of DNA and RNA
  • Excitation at 290nm & 490 nm
  • Emission at 530nm

DNA SafeStain is a new and safe nucleic acid stain for visualization of double-stranded DNA, single-stranded DNA, and RNA in agarose gels. The dyes are developed to replace toxic ethidium bromide (EthBr, a potent mutagen), commonly used in gel electrophoresis for visualization of nucleic acids in agarose gels. DNA SafeStain is non-carcinogenic by the Ames-test. The results are negative in both the mouse marrow chromophilous erthrocyte micronucleus and mouse spermary spermatocyte chromosomal aberration tests.

DNA SafeStain emits green fluorescence when bound to DNA. It has two excitation wavelength peaks when bound to nucleic acid, at 290nm and 490nm and the emission can be detected at 530nm.

Storage:
Store under dark at 4°C or room temperature.

User Instruction: 
DNA SafeStain can be used in the exact same way as EtBr in agarose gel electrophoresis. For example, add 100µl to 1 liter of 1X running buffer (1xTAE or 1x TBE), and use this DNA SafeStain containing buffer to make the agarose gel and run the gel.

 

Special Notes: 
1. For best results, dilute the DNA SafeStain 1:10,000 in your gel running buffer and use this dye-containing running buffer to make your agarose gel and to run the gel.

2. As is the case with EtBr; add more DNA SafeStain, if brighter DNA bands are desired. To view the results, any conventional DNA gel viewing light box or gel documentation system should work well with the DNA SafeStain; although, using a LED light instead of a UV light source is preferred due to the harmful nature of UV light.

This Product is For Research Use Only

For the proper disposal of this product, follow University or Company Guidelines.

 

Due to supply constraints 100-SEAL-PLT, STR-SEAL-PLT, XTR-100 and XTR-LG-100 are out of stock.


STR-SEAL-PLT alternative is GPS-PP-100.

PP-100 substrate is polypropylene instead of polyester for the STR-SEAL-PLT.  GPS-PP-100 is designed for the same application – ELISA/Incubation/General Storage.

100-SEAL-PLT alternative is TTS-100.

XTR-100 and XTR-LG-100 alternatives are XTR2-100 & XTR2-LG-100.
 

ELISA, Incubation, and Short Term Incubation Sealing Films

All films on this page are designed for ELISA, Incubation, and Short Term Incubation Sealing Films. If you are looking for something specific please consult our selection guides by attribute or application on this page. All films are certified DNase- & RNase-Free.

Specific film descriptions:

1x8 MiniStrips (GP-CS1X8-400 and GPS-CS1X8-400) are less expensive than full-size sheets when using only a partial plate. Designed for use with either 1x8 strip-well ELISA plates or standard 96-well plates where rows must be selectively protected or accessed. Ideal for COVID-19 testing to seal a row of 8 wells after swabs are deposited into a deep-well plate.

General Purpose (GP-PP-100 and GPS-PP-100) polypropylene lms oer superior barrier properties to minimize evaporation and prevent well-to-well contamination. Ideal for use with polypropylene plates.

Pre-scored lm (SP2-RL-500) on a roll is a more economical and ecient alternative to rapidly seal microplates or racked test tubes; faster than applying single sheeted lm. Self-standing, non-slip supports hold the pre-scored lm roll securely in place for quick and easy bench- top dispensement. The roll unwind tension can be easily adjusted to user preference. Continuous backing liner can be torn o or fed directly into a waste basket for disposal.

The SealMate SealPlate Starter Kit (SM-KIT-SP and replacement rolls) features a re-usable dispenser and two rolls of perforated lm. The dispenser facilitates the quick and easy dispensing of lm from a roll allowing microplates to be sealed three times faster than using sheeted lms. Cost-eective replacement rolls are sold separately. Ideal for use under the hood; the SealMate dispenser minimizes lm contamination.

SealPla te (100-SEAL-PLT and STR-SEAL-PLT) minimizes evaporation, prevents well-to-well contamination and spillage. Eliminates the edge eect which can occur with plate lids (e.g. greater evaporation in the outside plate wells).

SealPlate Color Tabs (SP-IDx-100) Each end-tab features a colored dot and white writing area. An inscribed color-coded end-tab can be removed and pasted into a laboratory notebook while the other remains on the plate for identication. The lm sheet can be removed from the plate without disturbing the identication label due to the nely perforated end-tabs. Choose from 5 dierent individual colors or the assortment pack.

SealPlate 2x8 MiniStrips (SP-2X8-50 and SPS-2X8-50) are less expensive than full-size sheets when using only a partial plate. Designed for use with either 1x8 strip- well ELISA plates or standard 96-well plates where rows must be selectively protected or accessed.

ValueSeal (SP-VS-100) features smaller dimensions and a single non-perforated end-tab to provide a more cost-eective alternative to protect samples from evaporation, external contamination, dust and particulates.

Gel Cutting Tips

  • Safely excise bands from gels
  • Avoid cross contamination
  • One-handed operation for quick and accurate gel cutting
  • Safer than using razor blades and won't scratch transilluminators
  • 1.1 x 4 mm
Cut gel with pipet tip on the end of a standard 1000µl pipettor. Gel piece is suspended in tip when pipettor is lifted from the gel. Expel the gel piece by pushing the pushbutton on the pipettor. Tip is ejected in normally using the ejector button.
 
Item#:
ASGELTIP1_1X4
5X RNA Gel Loading Kit
  • Reagents for denaturing and loading RNA samples onto a formaldehyde gel, using MOPS as a buffer
  • RNA sample is dissolved in 10µl of DEPC water and mixed with 35µl of denaturing solution. Heat the sample to 65°C for 5 min. Once the solution has cooled, add 5µl of loading dye. The sample is now ready to load into the gel.
  • DNase/RNase/Protease free
Item#:
IB01015
Your Price:
63.18
Each
Format, 30/cs, 25uL tubes for

The RotoCycler Discs are specially made to be used with the Qiagen Rotor-Gene Q Real-Time Rotary Analyzer. Two models are available: a 72-well format with 100 µL tubes and a 100-well format for reactions up to 25 µL. The discs a"one-piece "plate" equivalent, having vertically oriented wells compatible with automated reaction setup using a robotic liquid handling system.

Item#:
T319-100D1
Your Price:
302.16
Each
6X Loading Dye
    • Used for agarose electrophoresis of DNA, RNA or nucleic acids
    • Contains 3 tracking dyes and 15% Ficoll in a special Tris dye
      • Light blue - around 4000bp in 1% agarose
      • Indigo - around 600bp in 1% agarose
      • Magenta - around 150bp in 1% agarose
    • DNase/RNase/Protease free
Item#:
IB01010
Your Price:
72.26
Each
Discontinued, Contact us for more options!

This brand is being discontinued, please contact us for other options.

Need great dNTPs at a great price? Try our PR1MA™ dNTPs 

 

  • Mix of dATP, dCTP, dGTP, dTTP
  • Each nucleotide is at a concentration of 12.5 mM
  • Ready-to-use molecular grade dNTP solution for use in DNA polymerization, DNA labelling and sequencing processes
  • High purity: >98% by HPLC
  • Supplied in solution at pH 7.5
  • dNTPs are stable at -20°C, avoid multiple freeze/thawing (For long-term usage, aliquoting is recommended)
  • Functionally tested with thermostable polymerases
Item#:
ASPCRDNTP1
While Supplies Last!


This brand is being discontinued and will only be available while supplies last. 

Need great dNTPs at a great price?

Try our PR1MA™ dNTPs


 Bullseye Individual dNTP's

  • Ready-to-use molecular grade dNTP solution for use in DNA polymerization, DNA labeling and sequencing processes
  • High purity: >98% by HPLC
  • Supplied in solution at pH 7.5
  • dNTPs are stable at -20°C, avoid multiple freeze/thawing (For long-term usage, aliquoting is recommended)
  • Functionally tested with thermostable polymerases

Item#:
ASPCRDNTP4
While Supplies Last!



This brand is being discontinued and will only be available while supplies last.

 

Need great dNTPs at a great price?

Try our PR1MA™ dNTPs

  • Mix of dATP, dCTP, dGTP, dTTP
  • Each nucleotide is at a concentration of 100 mM
  • Ready-to-use molecular grade dNTP solution for use in DNA polymerization, DNA labelling and sequencing processes
  • High purity: > 98% by HPLC
  • Supplied in solution at pH 7.5
  • dNTPs are stable at - 20°C, avoid multiple freeze/thawing (For long-term usage, aliquoting is recommended)
  • Functionally tested with thermostable polymerases 

dNTP SET

(dATP, dCTP, dGTP, dTTP)

100 mM

Item #Size
BE51110920 x 4 x 250 uL
BE5111204 x 2 mL


Store at 20°C For in-vitro laboratory use only

Components Volume

dATP (100mM) 250 µL

dCTP (100mM) 250 µL

dGTP (100mM) 250 µL

dTTP (100mM) 250 µL

General Description

Ready-to-use molecular grade dNTP solution for use in DNA polymerization, DNA labelling and sequencing processes.

Features

High purity: >98% by HPLC.

Supplied in solution at pH 7.5.

Storage Conditions

dNTPs are stable at 20oC in a constant temperature freezer. Avoid multiple freeze/thawing. For long-term usage, aliquoting is recommended.

Quality control

Functionally tested with thermostable polymerases.

Item#:
ASPCRDNTP3
Discontinued, Contact us for more options!

This brand is being discontinued, please contact us for other options.
 

Need great dNTPs at a great price? Try our PR1MA™ dNTPs 
 



 
  • Mix of dATP, dCTP, dGTP, dTTP
  • Each nucleotide is at a concentration of 10 mM
  • Ready-to-use molecular grade dNTP solution for use in DNA polymerization, DNA labelling and sequencing processes
  • High purity: >98% by HPLC
  • Supplied in solution at pH 7.5
  • dNTPs are stable at -20°C, avoid multiple freeze/thawing (For long-term usage, aliquoting is recommended)
  • Functionally tested with thermostable polymerases
Item#:
ASPCRDNTP2
While Supplies Last!

This brand is being discontinued and will only be available while supplies last.

Need a great DNA Safe Stain at a great price? Try PR1MA!  Click here to order.
 
Bullseye DNA Safe Stain Plus


  • Higher sensitivity
  • Use in the same way as EtBr in agarose gel electrophoresis
  • Detection of DNA and RNA
  • Safest DNA stain by far
  • Low cost
  • 10,000X
  • Excitation wavelengths at 290nm and 490nm

 

DNA SafeStain Plus is a highly sensitive green fluorescent DNA/RNA staining reagent for detecting nucleic acids in agarose and polyacrylamide gels. This unique stain gives high sensitivity for detection of double-stranded or single-stranded DNA and RNA. Gels can be post-stained or the stain can be added to gels during gel casting or to the gel running buffer. DNA SafeStain Plus has two excitation wavelength peaks at about 290nm and 490nm, and an emission wavelength at 530nm, making it compatible with a standard UV light box, a blue-light transilluminator, or a gel reader equipped with visible light excitation; such as, a 488 nm laser-based gel scanner.


DNA SafeStain Plus is in a 10,000X concentrated format that can be easily diluted 10,000 times for use in precast gel staining, or 5,000 times for use in post gel staining.

Samples stained with DNA SafeStain Plus are compatible with downstream molecular biology applications; such as, gel extraction, and cloning
Plates, 10/pk, 5 pks/cs

Please know that the manufacturer has suspended manufacturing this product indefinitely. 
We suggest purchasing PR1MA PCR Plastics
.
 
Contact your rep or email tech@midsci.com for more information.



Sealing Mat for 96-Well PCR Plates

Reusable and versatile, Axygen AxyMats minimize evaporation in a wide range of temperatures in PCR and storage applications.

Item#:
AM-96-PCR-RD
Your Price:
324.51
Each
This item is discontinued
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