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PCR/qPCR
MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more. If you are looking for any of these things, we can match almost all needs to all cyclers. If you don’t find it easily contact us at tech@midsci.com and we will find it for you.
96 or 384 we have it. Non-Skirted, semi-skirted or full skirted, we have it. 0.1, 0.2, or 0.5 mL we have it. A1, A12, H12, A24, P24 cuts, we have them. Sybr© Green or probe alternative, we have it.
Pro Tip: Colored plastics do not have any impact on DNA amplification. But when setting up qPCR it is often recommended to use white plastics. This will limit or prevent fluorescence refraction. Light refraction can minimize sensitivity and consistency.
Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips. This will help optimize and reduce variance in the reactions. Simple test, see the residual liquid left in tips? This means some of the taq, dNTPs, sample, etc is left in the tip. PR1MA tips help reduce that significantly and improve your consistency.
Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate? Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler. Call us we have solutions.
We suggest purchasing PR1MA PCR Plastics.
Contact your rep or email tech@midsci.com for more information.
MIDSCI PCR 8-Strip Caps are compatible with MIDSCI PCR plates. The caps are available as a dome caps (AVT3878) or flat caps (AVT3799).
Specifications of MIDSCI strip caps:
- Highest standards of quality control
- Lot to Lot consistency
- Certified DNase/RNase, nucleic acid, PCR-inhibitor and pyrogen-free
- See fit chart for plate compatibility
We suggest purchasing PR1MA PCR Plastics.
Contact your rep or email tech@midsci.com for more information.
PCR Microplate, 96 Well
- Standard thermal cycling and qPCR compatible
- Ultra-thin and consistent wall thickness for precise thermal transfer
- Made from 99.9% virgin polypropylene
- Single notch corner
- 335ul well capacity (working capacity dependent on closure method)
- Fits standard thermal cyclers, ABI Real Time PCR 7300, 7500, 7900, qPCR and ABI Sequencing
- Certified DNAse/RNAse and pyrogen-safe
PCR Work-Up Rack fits PCR 0.2ml tubes, strip tubes or 96-well plates (including skirted plates). Polypropylene rack and lid click firmly for secure transportation and storage. Available in assorted colors: blue, green, yellow, violet and red.
- Plate Characteristics
- 96 x 0.1mL, Sub-skirted
- Low Profile (0.1mL), Frosted (low signal to noise ratio
- Extreme Uniformity (EU) Plate Design
- Uniform wall thickness ensures consistent even heating
- Thin wall-bottomed tube for rapid heat transfer
- Ideal paring with EU Caps with "Sealing Ring Band" Design
- Easy open - easy close with ergonomic caps: B57801, B79701, C79701
- Caps are significantly easier to apply and remove -- Repetitive Strain Injury (RSI) Friendly.
- Significant reduction in evaporation due to a wider sealing area.
- Certified DNA, DNase, RNA, RNase and pyrogen-free
Please give us a call for a sample!
Order# |
Description |
Non-Skirt |
|
B70501 |
Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, Natural, 25/cs |
B70509 |
Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, White, 25/cs |
B50501 |
Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, Frosted, 25/cs |
AB70651 |
Pryme PCR Plate, 96-Well, 0.1mL, Non-Skirt, Low Profile, Frosted, 25/cs |
Semi-Skirted |
|
B70651 |
Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, Natural, 25/cs |
B70659 |
Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, White, 25/cs |
B50651 |
Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, Frosted, 25/cs |
B17480 |
Pryme PCR Plate, 96-Well, 0.1mL, Semi-skirted, Low Profile, Frosted, 25/cs |
B17489 |
Pryme PCR Plate, 96-Well, 0.1mL, Semi-skirted, Low Profile, White, 25/cs |
Sub-Skirted |
|
ABI7501 |
Pryme PCR Plate, 96-Well, 0.2mL, Sub-skirted, Regular Profile, Frosted, 25/cs |
ABI7509 |
Pryme PCR Plate, 96-Well, 0.2mL, Sub-skirted, Regular Profile, White, 25/cs |
AB19800-EU |
Pryme PCR Plate, 96-Well, 0.1mL, Sub-skirted, Low Profile, Frosted, 25/cs |
AB19809-EU |
Pryme PCR Plate, 96-Well, 0.1mL, Sub-skirted, Low Profile, White, 25/cs |
Caps |
|
B57801 |
Pryme PCR Ergonomic 8-Strip, Optical Flat (V2) Caps, Natural, 120/cs |
B79701 |
Pryme PCR Ergonomic 8-Strip, Optical Flat (V1) Caps, Natural, 120/cs |
C79701 |
Pryme PCR Ergonomic 8-Strip, Semi-Domed (V1) Caps, Natural, 120/cs |
- Non-Skirt, low-profile (0.1ml)
- Cuttable into 16, 24, 32, or 48-well pieces
- White for optimal qPCR signal/noise ratios
- Thin-wall for consistent and accurate heat transfer
- Ultra-low sample evaporation
- Certified DNAse/RNAse, nucleic acid, PCR-inhibitor, and pyrogen-FREE
Closure can be accomplished with B57801 optical indented strip caps.
Click here to Order Now!
- Plate Characteristics
- 96 x 0.1mL, Sub-skirted, cuttable
- Low Profile (0.1mL), Frosted (low signal to noise ratio
- Extreme Uniformity (EU) Plate Design
- Uniform wall thickness ensures consistent even heating
- Thin wall-bottomed tube for rapid heat transfer
- Ideal paring with EU Caps with "Sealing Ring Band" Design
- Easy open - easy close with ergonomic caps: B57801, B79701, C79701
- Caps are significantly easier to apply and remove -- Repetitive Strain Injury (RSI) Friendly.
- Significant reduction in evaporation due to a wider sealing area.
- Certified DNA, DNase, RNA, RNase and pyrogen-free
Please give us a call for a sample!
Order# |
Description |
Non-Skirt |
|
B70501 |
Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, Natural, 25/cs |
B70509 |
Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, White, 25/cs |
B50501 |
Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, Frosted, 25/cs |
AB70651 |
Pryme PCR Plate, 96-Well, 0.1mL, Non-Skirt, Low Profile, Frosted, 25/cs |
Semi-Skirted |
|
B70651 |
Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, Natural, 25/cs |
B70659 |
Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, White, 25/cs |
B50651 |
Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, Frosted, 25/cs |
B17480 |
Pryme PCR Plate, 96-Well, 0.1mL, Semi-skirted, Low Profile, Frosted, 25/cs |
B17489 |
Pryme PCR Plate, 96-Well, 0.1mL, Semi-skirted, Low Profile, White, 25/cs |
Sub-Skirted |
|
ABI7501 |
Pryme PCR Plate, 96-Well, 0.2mL, Sub-skirted, Regular Profile, Frosted, 25/cs |
ABI7509 |
Pryme PCR Plate, 96-Well, 0.2mL, Sub-skirted, Regular Profile, White, 25/cs |
AB19800-EU |
Pryme PCR Plate, 96-Well, 0.1mL, Sub-skirted, Low Profile, Frosted, 25/cs |
AB19809-EU |
Pryme PCR Plate, 96-Well, 0.1mL, Sub-skirted, Low Profile, White, 25/cs |
Caps |
|
B57801 |
Pryme PCR Ergonomic 8-Strip, Optical Flat (V2) Caps, Natural, 120/cs |
B79701 |
Pryme PCR Ergonomic 8-Strip, Optical Flat (V1) Caps, Natural, 120/cs |
C79701 |
Pryme PCR Ergonomic 8-Strip, Semi-Domed (V1) Caps, Natural, 120/cs |
Pure•Amp™ Color Coded Strip Tubes
- RNase, DNase, DNA, and PCR inhibitor free
- 0.2mL or Low Profile 0.1mL options
- Ultra-thin, uniform wall thickness
- White and frosted tubes for enhanced qPCR results
We suggest purchasing PR1MA PCR Plastics.
Contact your rep or email tech@midsci.com for more information.
- Standard thermal cycling and qPCR compatible
- Ultra-thin and consistent wall thickness for precise thermal transfer
- Made from 99.9% virgin polypropylene
- Single notch corner
- 335ul well capacity (working capacity dependent on closure method)
- Fits standard thermal cyclers, ABI Real Time PCR 7300, 7500, 7900, qPCR and ABI Sequencing
- Certified DNAse/RNAse and pyrogen-safe
- The CleanSpot is designed to fulfill a very important laboratory need...control of PCR contamination.
- Just 25 - 30 minutes of UV irradiation in the CleanSpot can prevent unwanted PCR contamination.
- The combination of a special light reflector and two powerful overhead UV bulbs generate Pyrimidine Dimers (T,C) and other photo defects in contaminating target sequences, thus eliminating falsely primed products that result in countless lost time and expense.
- The CleanSpot is designed to prevent unwanted contaminant DNA in amplification mixtures, thereby saving many lost hours and expensive samples.
- The UV resistant acrylic enclosure provides a contained, dead air space in which to set up your reactions and permits safe 254nm UV irradiation of solutions and supplies. This powerful UV wavelength renders DNA contaminants biologically inactive in 30 minutes or less.
- The thick front panel is an effective beta irradiation shield as well as acting as a breath guard. An overhead fluorescent light illuminates the work area.
- Dimensions: 18"L x 24"W x 28"H
- 1 year warranty
The Rotocycler 72 facilitates the handling and insertion of caps on the tubes by offering a special solid aluminum loading rack. This rack can hold up to 18 x 0.1 mL tube strips for a total of 72 tubes. Other cavities can hold larger reaction tubes. To keep reactions cool during setup, simply place the rack in a refrigerated area. For easy reference, all wells are numbered. Color coding is made possible by inserting a Capinsert in up to two locations on the rack.
Includes: Rotocycler 72 and 500 assorted color coding inserts
Accessories for the Rotocycler 72
T319-4N |
Rotocycler 0.1 mL Tube and Cap Strips for Qiagen Rotor-Gene Q |
T319-72D2 |
Rotocycler 72 Rotor with 100 µL wells for Qiagen Rotor-Gene Q |
T319-100D1 |
Rotocycler 100 Rotor with 25 µL wells for Qiagen Rotor-Gene Q |
The RotoCycler Discs are specially made to be used with the Qiagen Rotor-Gene Q Real-Time Rotary Analyzer. Two models are available: a 72-well format with 100 µL tubes and a 100-well format for reactions up to 25 µL. The discs a"one-piece "plate" equivalent, having vertically oriented wells compatible with automated reaction setup using a robotic liquid handling system.