PCR/qPCR

MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more.  If you are looking for any of these things, we can match almost all needs to all cyclers.  If you don’t find it easily contact us at tech@midsci.com and we will find it for you. 

96 or 384 we have it.  Non-Skirted, semi-skirted or full skirted, we have it.  0.1, 0.2, or 0.5 mL we have it.  A1, A12, H12, A24, P24 cuts, we have them.  Sybr© Green or probe alternative, we have it.  

Pro Tip: Colored plastics do not have any impact on DNA amplification.  But when setting up qPCR it is often recommended to use white plastics.  This will limit or prevent fluorescence refraction.  Light refraction can minimize sensitivity and consistency. 

Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips.  This will help optimize and reduce variance in the reactions.  Simple test, see the residual liquid left in tips?  This means some of the taq, dNTPs, sample, etc is left in the tip.  PR1MA tips help reduce that significantly and improve your consistency. 

Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate?  Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler.  Call us we have solutions.

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96x0.1ml, Sub-Skirted,
This item has been discontinued and replaced by item 
  • Plate Characteristics
    • 96 x 0.1mL, Sub-skirted
    • Low Profile (0.1mL), Frosted (low signal to noise ratio
    • Extreme Uniformity (EU) Plate Design
    • Uniform wall thickness ensures consistent even heating
    • Thin wall-bottomed tube for rapid heat transfer
  • Ideal paring with EU Caps with "Sealing Ring Band" Design
    • Easy open - easy close with ergonomic caps: B57801, B79701, C79701
    • Caps are significantly easier to apply and remove -- Repetitive Strain Injury (RSI) Friendly.
    • Significant reduction in evaporation due to a wider sealing area.
  • Certified DNA, DNase, RNA, RNase and pyrogen-free

Please give us a call for a sample!

 

Order#

Description

Non-Skirt

 

B70501

Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, Natural, 25/cs

B70509

Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, White, 25/cs

B50501

Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, Frosted, 25/cs

AB70651

Pryme PCR Plate, 96-Well, 0.1mL, Non-Skirt, Low Profile, Frosted, 25/cs

Semi-Skirted

 

B70651

Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, Natural, 25/cs

B70659

Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, White, 25/cs

B50651

Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, Frosted, 25/cs

B17480

Pryme PCR Plate, 96-Well, 0.1mL, Semi-skirted, Low Profile, Frosted, 25/cs

B17489

Pryme PCR Plate, 96-Well, 0.1mL, Semi-skirted, Low Profile, White, 25/cs

Sub-Skirted

 

ABI7501

Pryme PCR Plate, 96-Well, 0.2mL, Sub-skirted, Regular Profile, Frosted, 25/cs

ABI7509

Pryme PCR Plate, 96-Well, 0.2mL, Sub-skirted, Regular Profile, White, 25/cs

AB19800-EU

Pryme PCR Plate, 96-Well, 0.1mL, Sub-skirted, Low Profile, Frosted, 25/cs

AB19809-EU

Pryme PCR Plate, 96-Well, 0.1mL, Sub-skirted, Low Profile, White, 25/cs

Caps

 

B57801

Pryme PCR Ergonomic 8-Strip, Optical Flat (V2) Caps, Natural, 120/cs

B79701

Pryme PCR Ergonomic 8-Strip, Optical Flat (V1) Caps, Natural, 120/cs

C79701

Pryme PCR Ergonomic 8-Strip, Semi-Domed (V1) Caps, Natural, 120/cs

Item#:
AB19800-EU
Your Price:
117.35
Each
This item is discontinued
Non-Skirted. Low-Profile,

  • Non-Skirt, low-profile (0.1ml)
  • Cuttable into 16, 24, 32, or 48-well pieces
  • White for optimal qPCR signal/noise ratios
  • Thin-wall for consistent and accurate heat transfer
  • Ultra-low sample evaporation
  • Certified DNAse/RNAse, nucleic acid, PCR-inhibitor, and pyrogen-FREE

 

Closure can be accomplished with B57801 optical indented strip caps.

Item#:
AB70659
Your Price:
171.43
Each
Non-Skirted, Low Profile,
Need a great PCR plastic and at a better price? Try PR1MA. 
 Click here to Order Now!
 
  • Plate Characteristics
    • 96 x 0.1mL, Sub-skirted, cuttable
    • Low Profile (0.1mL), Frosted (low signal to noise ratio
    • Extreme Uniformity (EU) Plate Design
    • Uniform wall thickness ensures consistent even heating
    • Thin wall-bottomed tube for rapid heat transfer
  • Ideal paring with EU Caps with "Sealing Ring Band" Design
    • Easy open - easy close with ergonomic caps: B57801, B79701, C79701
    • Caps are significantly easier to apply and remove -- Repetitive Strain Injury (RSI) Friendly.
    • Significant reduction in evaporation due to a wider sealing area.
  • Certified DNA, DNase, RNA, RNase and pyrogen-free

Please give us a call for a sample!

 

Order#

Description

Non-Skirt

 

B70501

Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, Natural, 25/cs

B70509

Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, White, 25/cs

B50501

Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, Frosted, 25/cs

AB70651

Pryme PCR Plate, 96-Well, 0.1mL, Non-Skirt, Low Profile, Frosted, 25/cs

Semi-Skirted

 

B70651

Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, Natural, 25/cs

B70659

Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, White, 25/cs

B50651

Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, Frosted, 25/cs

B17480

Pryme PCR Plate, 96-Well, 0.1mL, Semi-skirted, Low Profile, Frosted, 25/cs

B17489

Pryme PCR Plate, 96-Well, 0.1mL, Semi-skirted, Low Profile, White, 25/cs

Sub-Skirted

 

ABI7501

Pryme PCR Plate, 96-Well, 0.2mL, Sub-skirted, Regular Profile, Frosted, 25/cs

ABI7509

Pryme PCR Plate, 96-Well, 0.2mL, Sub-skirted, Regular Profile, White, 25/cs

AB19800-EU

Pryme PCR Plate, 96-Well, 0.1mL, Sub-skirted, Low Profile, Frosted, 25/cs

AB19809-EU

Pryme PCR Plate, 96-Well, 0.1mL, Sub-skirted, Low Profile, White, 25/cs

Caps

 

B57801

Pryme PCR Ergonomic 8-Strip, Optical Flat (V2) Caps, Natural, 120/cs

B79701

Pryme PCR Ergonomic 8-Strip, Optical Flat (V1) Caps, Natural, 120/cs

C79701

Pryme PCR Ergonomic 8-Strip, Semi-Domed (V1) Caps, Natural, 120/cs

Item#:
AB70651
Your Price:
109.68
Each
This item is discontinued

Pure•Amp™ Color Coded Strip Tubes

Pure•Amp™ 0.2mL PCR tubes and strips are available in seven different configurations including single tubes, eight strips, domed caps, and flat caps. Optically clear caps, and white tubes (for optimal fluorescence reflectivity) are also available for qPCR applications.

Pure•Amp™ 0.2 mL color coded PCR strip tubes are easily identified at a glance, making them the ideal tube to track your samples.

Pure•Amp™ 0.1mL Low Profile PCR tubes and strips are available in three different color/texture configurations, all intended for qPCR applications: Clear, White, and Frosted.

All Pure•Amp™ PCR Tubes and Strips are precision manufactured to maintain tight dimensional tolerances of ultra-thin wall thickness, for improved thermal transfer. Pure•Amp™ PCR Plastics are certified free of RNase, DNase, DNA and PCR inhibitors, and are made of heat resistant medical-grade polypropylene.
  • RNase, DNase, DNA, and PCR inhibitor free
  • 0.2mL or Low Profile 0.1mL options
  • Ultra-thin, uniform wall thickness
  • White and frosted tubes for enhanced qPCR results
Item#:
ASPUREAMPCLRTB
Clear, Flat Top, 25/cs
This item has been discontinued and replaced by item 
Please know that the manufacturer has suspended manufacturing this product indefinitely. 
We suggest purchasing PR1MA PCR Plastics.
Contact your rep or email tech@midsci.com for more information.

 
  • Standard thermal cycling and qPCR compatible
  • Ultra-thin and consistent wall thickness for precise thermal transfer
  • Made from 99.9% virgin polypropylene
  • Single notch corner
  • 335ul well capacity (working capacity dependent on closure method)
  • Fits standard thermal cyclers, ABI Real Time PCR 7300, 7500, 7900, qPCR and ABI Sequencing
  • Certified DNAse/RNAse and pyrogen-safe
Item#:
PCR-96-FLT-C
Your Price:
91.69
Each
This item is discontinued
  • The CleanSpot is designed to fulfill a very important laboratory need...control of PCR contamination.
  • Just 25 - 30 minutes of UV irradiation in the CleanSpot can prevent unwanted PCR contamination.
  • The combination of a special light reflector and two powerful overhead UV bulbs generate Pyrimidine Dimers (T,C) and other photo defects in contaminating target sequences, thus eliminating falsely primed products that result in countless lost time and expense.
  • The CleanSpot is designed to prevent unwanted contaminant DNA in amplification mixtures, thereby saving many lost hours and expensive samples.
  • The UV resistant acrylic enclosure provides a contained, dead air space in which to set up your reactions and permits safe 254nm UV irradiation of solutions and supplies. This powerful UV wavelength renders DNA contaminants biologically inactive in 30 minutes or less.
  • The thick front panel is an effective beta irradiation shield as well as acting as a breath guard. An overhead fluorescent light illuminates the work area.
  • Dimensions: 18"L x 24"W x 28"H
  • 1 year warranty
Item#:
CS01
Your Price:
2984.00
Each
This item is discontinued
with 500 Color Coding Inserts

The Rotocycler 72 facilitates the handling and insertion of caps on the tubes by offering a special solid aluminum loading rack. This rack can hold up to 18 x 0.1 mL tube strips for a total of 72 tubes. Other cavities can hold larger reaction tubes. To keep reactions cool during setup, simply place the rack in a refrigerated area. For easy reference, all wells are numbered. Color coding is made possible by inserting a Capinsert in up to two locations on the rack.


Includes: Rotocycler 72 and 500 assorted color coding inserts


Accessories for the Rotocycler 72


T319-4N

Rotocycler 0.1 mL Tube and Cap Strips for Qiagen Rotor-Gene Q 
Real-Time Rotary Analyzer

T319-72D2

Rotocycler 72 Rotor with 100 µL wells for Qiagen Rotor-Gene Q 
Real-Time Rotary Analyzer

T319-100D1

Rotocycler 100 Rotor with 25 µL wells for Qiagen Rotor-Gene Q 
Real-Time Rotary Analyzer

Item#:
T319-4WS1
Your Price:
552.42
Each
Format, 24/cs, 100uL tubes for

The RotoCycler Discs are specially made to be used with the Qiagen Rotor-Gene Q Real-Time Rotary Analyzer. Two models are available: a 72-well format with 100 µL tubes and a 100-well format for reactions up to 25 µL. The discs a"one-piece "plate" equivalent, having vertically oriented wells compatible with automated reaction setup using a robotic liquid handling system.

Item#:
T319-72D2
Your Price:
233.19
Each
1mL, 1 vial of 1000ul
This item has been discontinued and replaced by item 

Affordable price & shipped at room temperature.

 

A premixed, ready-to-use solution for efficient amplification of DNA templates by PCR.

  • 2X Eco-Taq MasterMix contains Taq DNA Polymerase, dNTPs, Mg2+ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR, as well as an inert loading dye.
  • This pre-mixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set up. To prepare the final PCR, only primers and template DNA need to be added.
  • The mix retains all features of Taq DNA Polymerase and can amplify DNA targets up to 5 kb (simple template).
  • The elongation velocity is 0.9~1.2kb/min (70~75°C).
  • It has 5' to 3' polymerase activity but lacks 3' to 5' exonuclease activity which results in a 3'-dA overhang PCR product.

 

Features:

  • Convenient: Just add primers and template DNA
  • High yields of PCR products with minimal optimization.
  • High efficiency: saves your time by simplifying the process
  • Reproducible: lower contamination risk and pipetting error.

    Applications:

  • High-throughput PCR.
  • Routine PCR with high reproducibility
  • Generation of PCR products for TA cloning

    Contents:
    2X Taq Mix 1ml

    Store at -20°C
    For research use only

Item#:
ECO-TAQ1
Your Price:
90.00
Each
This item is discontinued

MIDSCI ELISA & Short Term


The polyester films are a great general purpose seal; ideal for sample protection and elimination of well-to-well sample cross contamination. The acrylic adhesive offers a strong bond that seals tightly on polypropylene and polystyrene plates.

This seal is ideally suited for short term storage, Elisa assays, prevention of sample loss, and general lab usage.

The paper backing of this film is scored on both ends to allow for two handed (precise) placement on the plate.

For PCR applications see our standard PCR film or our Real Time PCR film.

For applications where accessing samples through the seal with pipet tips or a probe is required, see the pierceable aluminum (MID-PCR-200) film.

Certified free of RNase, DNase, Pyrogen, and nucleic-acid contamination.
 

Specifications: 

Length146.05 mm
Length (w/ end tabs removed)119.05 mm
Width79.38 mm
Overall Thickness3.1 mil
Adhesive Thickness1.1 mil
MaterialPolyester
AdhesiveMedical Grade Acrylic
Max. Temperature120 °C
Min. Temperature-40 °C
Primary UsagesElisa, Storage or Sample Cover
 
Item#:
ASMIDSCIELISA
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