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PCR/qPCR

MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more.  If you are looking for any of these things, we can match almost all needs to all cyclers.  If you don’t find it easily contact us at tech@midsci.com and we will find it for you. 

96 or 384 we have it.  Non-Skirted, semi-skirted or full skirted, we have it.  0.1, 0.2, or 0.5 mL we have it.  A1, A12, H12, A24, P24 cuts, we have them.  Sybr© Green or probe alternative, we have it.  

Pro Tip: Colored plastics do not have any impact on DNA amplification.  But when setting up qPCR it is often recommended to use white plastics.  This will limit or prevent fluorescence refraction.  Light refraction can minimize sensitivity and consistency. 

Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips.  This will help optimize and reduce variance in the reactions.  Simple test, see the residual liquid left in tips?  This means some of the taq, dNTPs, sample, etc is left in the tip.  PR1MA tips help reduce that significantly and improve your consistency. 

Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate?  Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler.  Call us we have solutions.

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5ml
6X Loading Dye
    • Used for agarose electrophoresis of DNA, RNA or nucleic acids
    • Contains 3 tracking dyes and 15% Ficoll in a special Tris dye
      • Light blue - around 4000bp in 1% agarose
      • Indigo - around 600bp in 1% agarose
      • Magenta - around 150bp in 1% agarose
    • DNase/RNase/Protease free
Item#:
IB01010
Your Price:
70.16
Each

Need great dNTPs at a great price? Try PR1MA!  Click here to order.

 

  • Mix of dATP, dCTP, dGTP, dTTP
  • Each nucleotide is at a concentration of 12.5 mM
  • Ready-to-use molecular grade dNTP solution for use in DNA polymerization, DNA labelling and sequencing processes
  • High purity: >98% by HPLC
  • Supplied in solution at pH 7.5
  • dNTPs are stable at -20°C, avoid multiple freeze/thawing (For long-term usage, aliquoting is recommended)
  • Functionally tested with thermostable polymerases
Item#:
ASPCRDNTP1
Need great dNTPs at a great price? Try PR1MA!  Click here to order.


 
  • Ready-to-use molecular grade dNTP solution for use in DNA polymerization, DNA labeling and sequencing processes
  • High purity: >98% by HPLC
  • Supplied in solution at pH 7.5
  • dNTPs are stable at -20°C, avoid multiple freeze/thawing (For long-term usage, aliquoting is recommended)
  • Functionally tested with thermostable polymerases
Item#:
ASPCRDNTP4
Need great dNTPs at a great price? Try PR1MA!  Click here to order.

  • Mix of dATP, dCTP, dGTP, dTTP
  • Each nucleotide is at a concentration of 100 mM
  • Ready-to-use molecular grade dNTP solution for use in DNA polymerization, DNA labelling and sequencing processes
  • High purity: >98% by HPLC
  • Supplied in solution at pH 7.5
  • dNTPs are stable at -20°C, avoid multiple freeze/thawing (For long-term usage, aliquoting is recommended)
  • Functionally tested with thermostable polymerases

 

 

dNTP SET

(dATP, dCTP, dGTP, dTTP)

100 mM

Cat. No.

Size

dNTP sets

each 100mM Solutions

BE511109

20x 4x 250 µL

dATP, dCTP, dGTP, dTTP

BE511120

4x 2 mL

dATP, dCTP, dGTP, dTTP

Store at 20°C For in-vitro laboratory use only

 

Components Volume

dATP (100mM) 250 µL

dCTP (100mM) 250 µL

dGTP (100mM) 250 µL

dTTP (100mM) 250 µL

General Description

Ready-to-use molecular grade dNTP solution for use in DNA polymerization, DNA labelling and sequencing processes.

Features

High purity: >98% by HPLC.

Supplied in solution at pH 7.5.

Storage Conditions

dNTPs are stable at 20oC in a constant temperature freezer. Avoid multiple freeze/thawing. For long-term usage, aliquoting is recommended.

Quality control

Functionally tested with thermostable polymerases.
Item#:
ASPCRDNTP3

Need great dNTPs at a great price? Try PR1MA!  Click here to order.



 
  • Mix of dATP, dCTP, dGTP, dTTP
  • Each nucleotide is at a concentration of 10 mM
  • Ready-to-use molecular grade dNTP solution for use in DNA polymerization, DNA labelling and sequencing processes
  • High purity: >98% by HPLC
  • Supplied in solution at pH 7.5
  • dNTPs are stable at -20°C, avoid multiple freeze/thawing (For long-term usage, aliquoting is recommended)
  • Functionally tested with thermostable polymerases
Item#:
ASPCRDNTP2

Bullseye DNA Safe Stain Plus


  • Higher sensitivity
  • Use in the same way as EtBr in agarose gel electrophoresis
  • Detection of DNA and RNA
  • Safest DNA stain by far
  • Low cost
  • 10,000X
  • Excitation wavelengths at 290nm and 490nm

 

DNA SafeStain Plus is a highly sensitive green fluorescent DNA/RNA staining reagent for detecting nucleic acids in agarose and polyacrylamide gels. This unique stain gives high sensitivity for detection of double-stranded or single-stranded DNA and RNA. Gels can be post-stained or the stain can be added to gels during gel casting or to the gel running buffer. DNA SafeStain Plus has two excitation wavelength peaks at about 290nm and 490nm, and an emission wavelength at 530nm, making it compatible with a standard UV light box, a blue-light transilluminator, or a gel reader equipped with visible light excitation; such as, a 488 nm laser-based gel scanner.


DNA SafeStain Plus is in a 10,000X concentrated format that can be easily diluted 10,000 times for use in precast gel staining, or 5,000 times for use in post gel staining.

Samples stained with DNA SafeStain Plus are compatible with downstream molecular biology applications; such as, gel extraction, and cloning
Item#:
ASBULLEYEDNAPLUS
Plates, 10/pk, 5 pks/cs

Please know that the manufacturer has suspended manufacturing this product indefinitely. 
We suggest purchasing PR1MA PCR Plastics.
 Contact your rep or email tech@midsci.com for more information.



Sealing Mat for 96-Well PCR Plates

Reusable and versatile, Axygen AxyMats minimize evaporation in a wide range of temperatures in PCR and storage applications.

Item#:
AM-96-PCR-RD
Your Price:
324.51
Each
This item is discontinued

eXTReme™ FoilSeal™


eXTReme FoilSeal is an aluminum foil seal that offers excellent chemical resistance and superior performance in ultra-low temperatures.

Application: Long-term storage, light sensitive assays and robotics
Temperature Range: -80°C to +120°C
 
The smaller dimensions and chamfered corners result in no plate overhang for robotics applications; universal fit for use with all plate types (e.g. raised-rim and non-skirted). Or select our larger cut for greater plate coverage: eXTReme FoilSeal, Large

  • Certified DNase- & RNase- free
  • Recommended for -80°C long-term storage
  • Excellent chemical resistance (e.g. DMSO)
  • Specially formulated adhesive provides an excellent seal to minimize evaporation and well-to-well contamination
  • 1.9mil thick aluminum foil removes easily without tearing and allows for visual verification of the seal’s security around each well
  • Pierceable with a pipet tip for direct sample recovery
  • Not recommended for PCR
 
Helpful Tips:

  • Remove diagonally from a corner to prevent tearing
  • Selection of the proper film size will minimize evaporation
  • More complete coverage of the plate will reduce evaporation in the outer wells
Item#:
ASEXTRFOILS
 
eXTReme FoilSealFeatures
Specs eXTRemeFoilSeal
Sealing film selection guide by application
Sealing film selection guide by attributes
Includes Writer and

Qamp Mini Portable PCR Thermocycler
  • Compact in Size: 102 x 136 x 104 mm
  • Preprogramed Chip: with unlimited applications
  • Touch Start: One Button to Go!
  • LCD Display: for customers to define status easily!
  • Portable: Only 1 kg

 

Qamp Mini is designed to achieve polymerase chain reaction (PCR) for the amplification of the targeted DNA in a miniature device making molecular biology more fun in the field. The Qamp Mini contains a centrally positioned Peltier heating and cooling module for 1-8 samples.  The design leads to accuracy in analysis and cost efficiency without sacrificing performance and quality.  With the compact size and One-Click to Go design Qamp Mini is the ideal instrument for laboratories or classrooms and in the fields of epidemiology, veterinary, food testing, pathogen detection, ecology, archaeology research and others.



Sample Number

8 (2 x 4 wells)

Temperature Range

4°C - 99°C

Max Heating Rate (°C/sec.)

4.6°C

Max Cooling Rate (°C/sec.)

3.4°C

Temperature Accuracy

± 0.4°C

Temperature Uniformity Across Block

± 0.4°C

Max No. of Cycles

unlimited

Max No. of Steps

unlimited

Display

LCD

Heated Lid

105°C Fixed (pre-heat to 60°C)

Dimensions (L x W x H)

10 x 13 x 10 cm

Weight

1 Kg

Power

VAC 100-240, 50/60 Hz, 120 W
Item#:
C310200
Your Price:
1687.50
Each
Item is no longer available.

EZcap™ PCR FilmStrips 


An economical and more user-friendly alternative to PCR strip-caps; no sore thumbs guaranteed!

Application: PCR and short-term storage
Temperature Range: -20°C to +120°C

Precision cut to a smaller dimension for sealing one PCR 8-tube strip or a single row of a 96-well plate whenever rows must be selectively protected or accessed. FilmStrips remove easily with no splash back as can occur with strip-caps.

  • Certified DNase- & RNase- free
  • Features an extra strong, thick adhesive to minimize evaporation
  • Each sheet contains 8 strips on a continuous liner
 
Helpful Tips:

  • A non-locking workstation is recommended for sample prep (e.g. 96-well round-boom plate)
  • Removal of the end-tabs at the perforations is recommended before thermal cycling
  • A final sealing step is highly recommended in the thermal cycler because 8-tube strips flex during handling
Item#:
ASEZCAFILM
 
EZcap PCR FilmStrips Features
Specs EZcap PCR FilmStrips
Sealing film selection guide by application
Sealing film selection guide by attributes

Need great (q)PCR Regents at a great price? Try PR1MA!  Click here to order.

Pfu 2x Master Mix 
Pfu DNA Polymerase 2x master mix is ready to use premix which contains Pfu DNA Polymerase, dNTPs, MgCl2 and stabilizers with optimized reaction buffer. It has been optimized for routine PCR applications. Pfu DNA Polymerase is a heat stable DNA polymerase which has 5' 3' DNA polymerase and 3' 5' exonuclease (proofreading) activities. Pfu DNA polymerase retains the high fidelity, sensitivity and processivity with an error rate six-fold lower than Taq DNA polymerase, and significantly lower than the error rates of most other proofreading enzymes or DNA polymerase mixtures. Pfu 2x Master Mix product is supplied with the unique Intact Genomics 5x Magic Enhancer that enables efficient amplification of GC rich templates up to 84%.

Quality Control 
Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product. Each lot is repeatedly compared side-by-side with leading competitors to ensure our products outperform the competitor before product launching. 

Product Source
E. coli strain expressing a Pfu DNA Polymerase gene from Pyrococcus furiosus.

Contents & Storage

  1. Pfu 2x master mix
  2. 5x Magic Enhancer

Store all contents at -20 °C.

1x Master Mix Composition 
10 mM Tris-HCl pH 9.0, 50 mM KCl, 1.5 mM MgCl, 0.2 mM dNTPs, 5% Glycerol, 0.08% Igepal CA 630, 0.05% Tween-20, 100 Units/ml Pfu DNA Polymerase.

Protocol
1. Prepare a reaction mix according to the following table:
  


PCR reaction set up:

Template DNA

1-50 ng

Forward primer (5 µM)

1.0µl

Reverse primer (5 µM)

1.0µl

Pfu 2x master mix

10.0µl

5x Magic Enhancer (optional)

4.0µl

HO up to

20.0µl

2. Mix the reaction mixture thoroughly.
3. Program the thermal cycler according to the manufacturer's instructions.
4. A typical PCR cycling program is outlined in the following table.

 


PCR cycling conditions:

Steps

Temp.

Time

Cycles 

Initial Denaturation

95 °C

3 min

1

Denaturation

95 °C

30 sec 

 25-40

Annealing

50-66 °C

30 sec 

Extension

72 °C

1 min/kb

Final Extension

72 °C

5 min 

1

Hold 

4-12 °C



5. Place the PCR tubes in the thermal cycler and start the cycling program.

6. Analyze 5 µl of PCR products by agarose gel electrophoresis. 

Item#:
ASPCRREAG16

Need great (q)PCR Regents at a great price? Try PR1MA!  Click here to order.


Pfu DNA Polymerase 
Pfu DNA polymerase is a heat stable DNA polymerase which has 5' 3' DNA polymerase and 3' 5' exonuclease (proofreading) activities. Pfu DNA polymerase retains the high fidelity, sensitivity and processivity with an error rate six-fold lower than Taq DNA polymerase, and significantly lower than the error rates of most other proofreading enzymes or DNA polymerase mixtures (1). This product is supplied with the unique Intact Genomics 10x PCR reaction buffer, containing MgCl2, which produces a final Mg2+ concentration of 1.5 mM, and 5X Magic Enhancer that enables efficient amplification of GC rich templates up to 84%. The physical purity of this enzyme is 98% as assessed by SDS-PAGE with Coomassie® blue staining.

Quality Control 
Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product. Each lot is repeatedly compared side-by-side with leading competitors to ensure our products outperform the competitor before product launching. 

Product Source
E. coli strain expressing a Pfu DNA Polymerase gene from Pyrococcus furiosus.

Contents & Storage

  1. Pfu DNA Polymerase
  2. 10x PCR Buffer with Mg²+
  3. 5x Magic Enhancer
  4. 10 mM dNTP (Cat. # 3312d, 3314d only)

Store all contents at -20 °C.

Storage Buffer 
50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.5 @ 25 °C.

10X PCR Buffer with Mg2+ 
100 mM Tris-HCl pH 9.0, 15 mM MgCl, 100 mM KCl, 80 mM (NH4)2SO4, 0.5% Igepal CA 630

Unit Definition 
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP into acid-insoluble form in 30 minutes at 72 °C.

Protocol
1. Thaw 10x PCR Buffer, dNTP mix, primer solutions, 5x Magic Enhancer (if required) and mix thoroughly before use. 
2. Prepare a reaction mix according to the following table: The reaction mix typically contains all the components needed for PCR except the template DNA.

 

PCR reaction set up:

Template DNA

xµl (0.01-0.5µMg)

10x PCR Buffer

10.0µl

dNTP (10 mM)

2.0µl

Forward Primer

xµl (0.1- 0.5µMM)

Reverse Primer

xµl (0.1- 0.5µMM)

5x Magic Enhancer (optional)

20µl

Pfu DNA Polymerase (5 U/µl)

0.5µl

H2O up to

100.0µl

3. Mix the reaction mixture thoroughly.
4. Add template DNA to the individual PCR tubes containing the reaction mixture. 
5. Program the thermal cycler according to the manufacturerâs instructions. A typical PCR cycling program is outlined in the following table.

PCR cycling conditions:

Steps

Temp.

Time

Cycles 

Initial denaturation

95 °C

3-5 min

1

Denaturation

94 °C

30-60 sec 

  

25-35

Annealing

52-66 °C

30-60 sec 

Extension

72-74 °C

1-2 min 

Final extension

72-74 °C

10 min 

1

Hold 

4-12 °C

â
6. Place the PCR tubes in the thermal cycler and start the cycling program.
Item#:
ASPCRREAG17
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