This brand is being discontinued and will only be available while supplies last.
 Need a great DNA Safe Stain at a great price? Try PR1MA!  Click here to order.

• Safest DNA stain by far
• Replaces EthBr in agarose gel electrophoresis
• Detection of DNA and RNA
• Excitation at 290nm & 490 nm
• Emission at 530nm

DNA SafeStain is a new and safe nucleic acid stain for visualization of double-stranded DNA, single-stranded DNA, and RNA in agarose gels. The dyes are developed to replace toxic ethidium bromide (EthBr, a potent mutagen), commonly used in gel electrophoresis for visualization of nucleic acids in agarose gels. DNA SafeStain is non-carcinogenic by the Ames-test. The results are negative in both the mouse marrow chromophilous erthrocyte micronucleus and mouse spermary spermatocyte chromosomal aberration tests.

DNA SafeStain emits green fluorescence when bound to DNA. It has two excitation wavelength peaks when bound to nucleic acid, at 290nm and 490nm and the emission can be detected at 530nm.

Storage:
Store under dark at 4°C or room temperature.

User Instruction: 
DNA SafeStain can be used in the exact same way as EtBr in agarose gel electrophoresis. For example, add 100µl to 1 liter of 1X running buffer (1xTAE or 1x TBE), and use this DNA SafeStain containing buffer to make the agarose gel and run the gel.

Special Notes: 
1. For best results, dilute the DNA SafeStain 1:10,000 in your gel running buffer and use this dye-containing running buffer to make your agarose gel and to run the gel.

2. As is the case with EtBr; add more DNA SafeStain, if brighter DNA bands are desired. To view the results, any conventional DNA gel viewing light box or gel documentation system should work well with the DNA SafeStain; although, using a LED light instead of a UV light source is preferred due to the harmful nature of UV light.

This Product is For Research Use Only

For the proper disposal of this product, follow University or Company Guidelines.

Due to supply constraints 100-SEAL-PLT, STR-SEAL-PLT, XTR-100 and XTR-LG-100 are out of stock.


STR-SEAL-PLT alternative is GPS-PP-100.

PP-100 substrate is polypropylene instead of polyester for the STR-SEAL-PLT.  GPS-PP-100 is designed for the same application – ELISA/Incubation/General Storage.

100-SEAL-PLT alternative is TTS-100.

XTR-100 and XTR-LG-100 alternatives are XTR2-100 & XTR2-LG-100.
 

ELISA, Incubation, and Short Term Incubation Sealing Films

All films on this page are designed for ELISA, Incubation, and Short Term Incubation Sealing Films. If you are looking for something specific please consult our selection guides by attribute or application on this page. All films are certified DNase- & RNase-Free.

Specific film descriptions:

1x8 MiniStrips (GP-CS1X8-400 and GPS-CS1X8-400) are less expensive than full-size sheets when using only a partial plate. Designed for use with either 1x8 strip-well ELISA plates or standard 96-well plates where rows must be selectively protected or accessed. Ideal for COVID-19 testing to seal a row of 8 wells after swabs are deposited into a deep-well plate.

General Purpose (GP-PP-100 and GPS-PP-100) polypropylene lms oer superior barrier properties to minimize evaporation and prevent well-to-well contamination. Ideal for use with polypropylene plates.

Pre-scored lm (SP2-RL-500) on a roll is a more economical and ecient alternative to rapidly seal microplates or racked test tubes; faster than applying single sheeted lm. Self-standing, non-slip supports hold the pre-scored lm roll securely in place for quick and easy bench- top dispensement. The roll unwind tension can be easily adjusted to user preference. Continuous backing liner can be torn o or fed directly into a waste basket for disposal.

The SealMate SealPlate Starter Kit (SM-KIT-SP and replacement rolls) features a re-usable dispenser and two rolls of perforated lm. The dispenser facilitates the quick and easy dispensing of lm from a roll allowing microplates to be sealed three times faster than using sheeted lms. Cost-eective replacement rolls are sold separately. Ideal for use under the hood; the SealMate dispenser minimizes lm contamination.

SealPla te (100-SEAL-PLT and STR-SEAL-PLT) minimizes evaporation, prevents well-to-well contamination and spillage. Eliminates the edge eect which can occur with plate lids (e.g. greater evaporation in the outside plate wells).

SealPlate Color Tabs (SP-IDx-100) Each end-tab features a colored dot and white writing area. An inscribed color-coded end-tab can be removed and pasted into a laboratory notebook while the other remains on the plate for identication. The lm sheet can be removed from the plate without disturbing the identication label due to the nely perforated end-tabs. Choose from 5 dierent individual colors or the assortment pack.

SealPlate 2x8 MiniStrips (SP-2X8-50 and SPS-2X8-50) are less expensive than full-size sheets when using only a partial plate. Designed for use with either 1x8 strip- well ELISA plates or standard 96-well plates where rows must be selectively protected or accessed.

ValueSeal (SP-VS-100) features smaller dimensions and a single non-perforated end-tab to provide a more cost-eective alternative to protect samples from evaporation, external contamination, dust and particulates.

PR1MA™ Tn5 2.0 Transposase

PR1MA™ Tn5 2.0 Transposase (Tnp) is a hyperactive retroviral integrase engineered for improved activity, speed, and robustness that is used to construct random next-generation sequencing libraries and to study chromatin structure using targeted ATAC-seq. PR1MA™ Tn5 2.0 can be used to randomly fragment any target and insert unique oligonucleotide adapters in a single reaction which reduces the time and sample requirements relative to traditional next-gen sequencing library construction. 

• Optimal temperature: 55°C
• Inactivation: 40X Stop solution included (2% SDS)
• Storage temperature: -20°C
• 10X Tn5 reaction buffer included

Buffer composition

• 100 mM Tris-HCl
• 100 mM MgCl2
• pH = 7.5

*These products are intended for research use only, not for diagnostic use. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.   
 

Gel Cutting Tips, 1.1 x 4mm

• Safely excise bands from gels
• Avoid cross contamination
• One-handed operation for quick and accurate gel cutting
• Safer than using razor blades and won't scratch transilluminators

X-Pierce™ Sealing Films for Re-sealability and Easy Access Without Pipet Tip Coring or Adhesive Fouling

A pre-cut “X” over each well creates bendable flaps that provide sample access without coring or adhesive fouling of tips or probes. Ideal for COVID-19 sample collection in deep-well plates using swabs.

• Pierceable
• Printed circles aid in the alignment of the film to the plate
• Printed alphanumerics identify each well of a 96-well plate
• Reseals after piercing for continued sample protection

Helpful Tips

• To help position the film to the plate, align wells A-1 and H-12

Applications

• Short-term processing and storage at ambient temperatures
• Robotics

Temperature Range

• -40°C to +90°C

AeraSeal™ SealMate™ Rolls for Fast and Efficient Microplate Sealing

ValueSeal™ Sealing Film

BrightMax™ White, Light-reflecting, Vinyl Sealing Film

Designed for use with white microplates.

• The light reflective properties on clear-bottom plates maximize the sensitivity of bioluminescence and chemiluminescence assays in bottom-reading luminometers
• Maximizes intensity and signal-to-noise ratios in luminescence assays
• May be used as a backing matrix for microscopy of punch-out samples for filter plates (e.g. ELISPOT assays)
• Features a single split liner for easy application

Applications

• Luminescence
• Fluorescence
• Microscopy

• -40°C to +80°C

TiterSeal™ Sealing Film

SealPlate® ColorTab™ Sealing Film for Sample Identification and Plate Labeling

An inscribed color-coded end-tab can be removed and pasted into a laboratory notebook while the other remains on the plate for identification. The film sheet can be removed from the plate without disturbing the identification label due to the finely perforated end-tabs. Choose from 5 different individual colors or the assortment pack.

• Provides a secure seal and is easy to remove
• Rigid polyester film features good clarity and easy handling
• Features two end-tabs with perforations

Applications

• General laboratory use
• Short-term storage
• Incubation
• ELISA

Temperature Range

• -40°C to +120°C

PR1MA™ Taq DNA Polymerase

Saves time and cost by enabling direct PCR amplification of unpurified templates. PR1MA™ Taq DNA polymerase is a recombinant, truncated (lacks 5’ to 3’ exonuclease activity), highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme is thermostable up to 98°C for polymerase chain reaction assays. It is supplied with 4 M betaine to improve amplification of GC-rich DNA and 30% sucrose to improve amplification from inhibitor-rich substrates such as blood.

• Lacks exonuclease activity
• Thermotolerant up to 98°C
• Resistant to inhibitors, e.g., whole blood
• Ideal for GC-rich templates
• Storage temperature: -20°C
• 10X PR1MA™ buffer, 4 M Betaine, and 30% Sucrose included

Buffer composition

• 50% glycerol
• 50 mM Tris-HCl
• 50 mM KCl
• 1 mM DTT
• 0.1  mM EDTA
• 0.5% Tween-20
• 0.5% NP-40 substitute
• pH = 7.5 

Important note: Please be sure to use the buffer provided with this product to ensure optimal results.

*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.