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PCR/qPCR

MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more.  If you are looking for any of these things, we can match almost all needs to all cyclers.  If you don’t find it easily contact us at tech@midsci.com and we will find it for you. 

96 or 384 we have it.  Non-Skirted, semi-skirted or full skirted, we have it.  0.1, 0.2, or 0.5 mL we have it.  A1, A12, H12, A24, P24 cuts, we have them.  Sybr© Green or probe alternative, we have it.  

Pro Tip: Colored plastics do not have any impact on DNA amplification.  But when setting up qPCR it is often recommended to use white plastics.  This will limit or prevent fluorescence refraction.  Light refraction can minimize sensitivity and consistency. 

Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips.  This will help optimize and reduce variance in the reactions.  Simple test, see the residual liquid left in tips?  This means some of the taq, dNTPs, sample, etc is left in the tip.  PR1MA tips help reduce that significantly and improve your consistency. 

Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate?  Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler.  Call us we have solutions.

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Item#:
AS48RND3P4ML

SEP-R-8™ Breakable PCR Plates

 

Sep-R-8™ Breakable PCR plates can be broken down into strips of eight tubes for greater control over sample quantity and reduced waste.

 

PureAmp™ 0.2mL PCR Plates are designed to be compatible with the most popular thermal cyclers.  All PureAmp™ plates are held to very tight dimensional tolerances with ultra-thin walls for optimal thermal transfer.

 

All PureAmp™ PCR Plates are free of RNase, DNase, DNA, and PCR inhibitors, and are made of heat-resistant medical-grade polypropylene.

 

Features:

  • Breaks into strips of 8 tubes
  • RNase, DNase, DNA, and PCR inhibitor free
  • Compatible with standard equipment
  • Ultra-thin walled tube wells
  • Alpha-numeric reference grids

 

Item#:
ASSEPR8BREAKPCR
Flat, Clear, 250/bg
This item has been discontinued and replaced by item 

Please know that the manufacturer has suspended manufacturing this product indefinitely.
We suggest purchasing PR1MA PCR Plastics.
Contact your rep or email tech@midsci.com for more information.


MIDSCI PCR 8-Strip Caps are compatible with MIDSCI PCR plates. The caps are available as a dome caps (AVT3878) or flat caps (AVT3799).
Specifications of MIDSCI strip caps:

  • Highest standards of quality control
  • Lot to Lot consistency
  • Certified DNase/RNase, nucleic acid, PCR-inhibitor and pyrogen-free
  • See fit chart for plate compatibility
Item#:
AVT3799
Item is no longer available.

Adjustable Well Plate Stand

U of M: Each

Achieves accurate pipetting by allowing the flexibility to adjust the angle of the microplate (45° max. tilt). The stand takes up little benchtop space measuring only 6"L x 4.50"W x 2.50"H.

(Well Orienters are sold separately)
Item#:
ASWELLPLATESTAND
Item#:
ASPCR96ELEV2

U of M: Each

Achieves accurate pipetting by allowing the flexibility to adjust the angle of the microplate (45° max. tilt). The stand takes up little benchtop space measuring only 6"L x 4.50"W x 2.50"H.

(Well Orienters are sold separately)

Item#:
WPAJ-1000
Your Price:
96.41
Each
Item#:
ASPCRHALFSKIRT

SureFrame™ Two-Component PCR Plates


SureFrame™ plates are made with a special two material process that creates an ultra-rigid polycarbonate frame, while maintaining the favorable thermal transfer characteristics of thin-walled polypropylene tube wells. This ultra-rigid frame is not only ideal for all automated platforms and plate grippers, but also increases the uniformity of your experiments. Traditional single-material plates have been shown to warp inside thermal cyclers. This deformation of the plates causes increased evaporation in the outer wells of the plate, leading to inconsistent amplification across wells. SureFrame’s polycarbonate frame eliminates warping, for completely uniform results.

  • Two material design for increased rigidity
  • Ideal for automated platforms
  • Reduces evaporation
  • Certified for PCR 
Item#:
ASSUREFRAMETWOP
Item#:
ASPCR96H0P1
Item#:
ASR96SEALMAT
Need a great PCR plastic and at a better price? Try PR1MA. 
 Click here to Order Now!
 
  • Compatible with most robotics, designed to SBS standards.
  • Rigid skirt for gripping by robotic plate handlers.
  • Minimal warp and shrinkage after thermal cycling
  • Compatible with ABI 3730 Automated Sequencers- single cut corner!
Item#:
PCRPLATES2
Please know that the manufacturer has suspended manufacturing this product indefinitely. 
We suggest purchasing PR1MA PCR Plastics.
Contact your rep or email tech@midsci.com for more information.

 
  • Ergonomic Plate Design
    • Easy to see black alphanumeric labeling
    • Cut Corner A12
  • Ideal paring with Caps
    • Flat 8-strip (AVST-01)
    • Domed 8-strip (AVSTC-N)
    • Domed 12-strip (AVSTC-12)
    • Additional Caps
  • Product certified free from RNase, DNase, DNA, PCR inhibitors and endotoxin

Order#

Description

Non-Skirted

 

AVRT-N

Pryme PCR Ergonomic Plate, 96-Well, 0.2 mL, Non-Skirted, Regular Profile, Natural, 25/cs

Semi-Skirted

 

AVRT1

Pryme PCR Ergonomic Plate, 96-Well, 0.2 mL, Semi-skirted, Regular Profile, Natural, 25/cs

Skirted

 

AVSK96

Pryme PCR Ergonomic Plate, 96-Well, 0.1 mL, Skirted, Regular Profile, Natural, 25/cs

Caps

 

AVST-01 

Pryme PCR Ergonomic 8-Strip, Flat Caps, Natural, 125/cs

AVSTC-N

Pryme PCR Ergonomic 8-Strip, Dome Caps, Natural, 125/cs

AVSTC-12

Pryme PCR Ergonomic 12-Strip, Dome Caps, Natural, 80/cs

Please give us a call for a sample!

Item#:
PCRPLATES3
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