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PCR/qPCR
MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more. If you are looking for any of these things, we can match almost all needs to all cyclers. If you don’t find it easily contact us at tech@midsci.com and we will find it for you.
96 or 384 we have it. Non-Skirted, semi-skirted or full skirted, we have it. 0.1, 0.2, or 0.5 mL we have it. A1, A12, H12, A24, P24 cuts, we have them. Sybr© Green or probe alternative, we have it.
Pro Tip: Colored plastics do not have any impact on DNA amplification. But when setting up qPCR it is often recommended to use white plastics. This will limit or prevent fluorescence refraction. Light refraction can minimize sensitivity and consistency.
Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips. This will help optimize and reduce variance in the reactions. Simple test, see the residual liquid left in tips? This means some of the taq, dNTPs, sample, etc is left in the tip. PR1MA tips help reduce that significantly and improve your consistency.
Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate? Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler. Call us we have solutions.
PR1MA™ Cod Uracil-DNA Glycosylase
PR1MA™ Cod Uracil-DNA Glycosylase (cUNG) is a recombinant, thermolabile enzyme that removes uracil from DNA. It is ideal for preventing carry over contamination during RNA or DNA amplification reactions that substitute dUTP for dTTP. cUNG is the only commercially available UNG that is completely and irreversibly inactivated by moderate heat treatment, unlike bacterial versions of the enzyme. Cod UNG treatment in combination with targeted pre-amplification using dUTP provides a simple and efficient solution to eliminate carry-over contamination and the generation of false positives and inaccurate quantification.
- Optimal temperature: 37°C
- Heat inactivation: 55°C for 5 minutes
- Enables contamination control in PCR and other amplification methods
- Does not degrade product after inactivation, enabling downstream use of the amplicon
- Storage temperature: -20°C
Buffer composition
- 50% glycerol
- 50 mM Tris-HCl
- 50 mM KCl
- 1 mM DTT
- 0.1 mM EDTA
- 0.1% Tween-20
- pH = 7.5
*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.
The Rotocycler 72 facilitates the handling and insertion of caps on the tubes by offering a special solid aluminum loading rack. This rack can hold up to 18 x 0.1 mL tube strips for a total of 72 tubes. Other cavities can hold larger reaction tubes. To keep reactions cool during setup, simply place the rack in a refrigerated area. For easy reference, all wells are numbered. Color coding is made possible by inserting a Capinsert in up to two locations on the rack.
Includes: Rotocycler 72 and 500 assorted color coding inserts
Accessories for the Rotocycler 72
T319-4N |
Rotocycler 0.1 mL Tube and Cap Strips for Qiagen Rotor-Gene Q |
T319-72D2 |
Rotocycler 72 Rotor with 100 µL wells for Qiagen Rotor-Gene Q |
T319-100D1 |
Rotocycler 100 Rotor with 25 µL wells for Qiagen Rotor-Gene Q |
FoilSeal™ RollMate™ Dispenser +1 Roll of FoilSeal™ Film, 500 Pre-Scored Sheets
- Certified DNase- & RNase- free
- Pierceable with a pipet tip or robotic probe for direct sample recovery
- 2.0mil aluminum foil removes easily without tearing
- The conformable nature of foil allows for the visual verification of the seal’s security around each well
- Not recommended for long-term cold storage
- PCR
- Light sensitive assays
- Short-term storage
- Robotics
- -20°C to +120°C
- End-tab removal before thermal cycling is highly recommended
- Remove diagonally from a corner to prevent tearing
ThermalSeal® 2™ and ThermalSeal® Advanced Sealing Film
- Certified DNase- & RNase- free
- Ideal for use with flat-top, semi-skirted and full-skirted PCR plates
- Compatible with hard-shell dual component (e.g. polycarbonate/polypropylene) PCR plates
- Large cut allows for easier positioning of the film to the plate via edge of plate alignment vs. centering
- Available in a smaller cut for raised-rim and non-skirted PCR plates: ThermalSeal® Advanced
- PCR
- -40°C to +120°C
- Selection of the proper film size will minimize evaporation
- The film should not overhang or ride up the wall of a raised-rim plate
- More complete coverage of skirted flat-top plates will reduce evaporation in the outer wells
- End-tab removal before thermal cycling is highly recommended
AbsorbMax™ Black Vinyl Sealing Film for Black Microplates
AbsorbMax™ is a black vinyl film that can be applied to either the top or bottom of the plate.- Minimizes background fluorescence and cross talk between plate wells
- Ideal as a back seal for black plates with clear well bottoms for top reading measurements
- Absorbs stray light, reducing the background caused by light scatter
- Protects light sensitive samples from degradation
- Features a single split liner for easy application
Applications
- Fluorescence assays
- Light sensitive sample protection
Temperature Range
- -40°C to +80°C
Zone-Free™
Zone-Free is a multilayer film consisting of a top pierceable clear polyethylene film and a white polypropylene sublayer with 96 circular areas void of adhesive.
- Pierceable
- Offers excellent chemical resistance
- Samples have minimal contact with adhesive due to the adhesive-free zones
The RotoCycler Discs are specially made to be used with the Qiagen Rotor-Gene Q Real-Time Rotary Analyzer. Two models are available: a 72-well format with 100 µL tubes and a 100-well format for reactions up to 25 µL. The discs a"one-piece "plate" equivalent, having vertically oriented wells compatible with automated reaction setup using a robotic liquid handling system.
Affordable price & shipped at room temperature.
A premixed, ready-to-use solution for efficient amplification of DNA templates by PCR.
- 2X Eco-Taq MasterMix contains Taq DNA Polymerase, dNTPs, Mg2+ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR, as well as an inert loading dye.
- This pre-mixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set up. To prepare the final PCR, only primers and template DNA need to be added.
- The mix retains all features of Taq DNA Polymerase and can amplify DNA targets up to 5 kb (simple template).
- The elongation velocity is 0.9~1.2kb/min (70~75°C).
- It has 5' to 3' polymerase activity but lacks 3' to 5' exonuclease activity which results in a 3'-dA overhang PCR product.
Features:
- Convenient: Just add primers and template DNA
- High yields of PCR products with minimal optimization.
- High efficiency: saves your time by simplifying the process
- Reproducible: lower contamination risk and pipetting error.
Applications:
- High-throughput PCR.
- Routine PCR with high reproducibility
- Generation of PCR products for TA cloning
Contents:
2X Taq Mix 1ml
Store at -20°C
For research use only
MIDSCI ELISA & Short Term
Specifications:
| Length | 146.05 mm |
| Length (w/ end tabs removed) | 119.05 mm |
| Width | 79.38 mm |
| Overall Thickness | 3.1 mil |
| Adhesive Thickness | 1.1 mil |
| Material | Polyester |
| Adhesive | Medical Grade Acrylic |
| Max. Temperature | 120 °C |
| Min. Temperature | -40 °C |
| Primary Usages | Elisa, Storage or Sample Cover |
ThermalSeal® An Easier to Remove Sealing Film
ThermalSeal® features a moderate strength adhesive for ease of removal.
- Certified DNase- & RNase- free
- Only recommended for structurally rigid polypropylene PCR plates that don’t warp
- A less rigid polypropylene film that minimizes aerosols upon removal
- Features good chemical resistance
- PCR
- General storage
- -40°C to +120°C
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