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PCR/qPCR

MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more.  If you are looking for any of these things, we can match almost all needs to all cyclers.  If you don’t find it easily contact us at tech@midsci.com and we will find it for you. 

96 or 384 we have it.  Non-Skirted, semi-skirted or full skirted, we have it.  0.1, 0.2, or 0.5 mL we have it.  A1, A12, H12, A24, P24 cuts, we have them.  Sybr© Green or probe alternative, we have it.  

Pro Tip: Colored plastics do not have any impact on DNA amplification.  But when setting up qPCR it is often recommended to use white plastics.  This will limit or prevent fluorescence refraction.  Light refraction can minimize sensitivity and consistency. 

Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips.  This will help optimize and reduce variance in the reactions.  Simple test, see the residual liquid left in tips?  This means some of the taq, dNTPs, sample, etc is left in the tip.  PR1MA tips help reduce that significantly and improve your consistency. 

Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate?  Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler.  Call us we have solutions.

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Item#:
ASADSEALP3P1

AlumaSeal® CS™


With a 50 µm layer of acrylic adhesive that withstands cold storage at temperatures to -80 °C.

Unlike other sealing films in the AlumaSeal group, AlumaSeal CS sealing films are not recommended for PCR or thermocycling. A single non-perforated end tab simplifies application. Simply hold the tab and strip the backing from the body of the sealing film as it lays on the plate to avoid curling. Dimensions are 82.6 by 132.6 mm, including the single 9.5 mm end tab. AlumaSeal CS sealing films are certified DNase-, RNase-, and nucleic-acid-free.

  • Specially formulated for cold storage to -80 °C
  • Excellent barrier properties to retard evaporation
  • Easily pierceable with pipet tips or robotic probes for sample recovery
 
*Completing application of AlumaSeal CS sealing films by pressing with the accessory plate roller assures a secure uniform seal around all wells.
Item#:
ASALUMASEL
 
AlumaSeal CS Features
Specs AlumaSeal CS
Sealing film selection guide by application
Sealing film selection guide by attributes
with 500 Color Coding Inserts

The Rotocycler 72 facilitates the handling and insertion of caps on the tubes by offering a special solid aluminum loading rack. This rack can hold up to 18 x 0.1 mL tube strips for a total of 72 tubes. Other cavities can hold larger reaction tubes. To keep reactions cool during setup, simply place the rack in a refrigerated area. For easy reference, all wells are numbered. Color coding is made possible by inserting a Capinsert in up to two locations on the rack.


Includes: Rotocycler 72 and 500 assorted color coding inserts


Accessories for the Rotocycler 72


T319-4N

Rotocycler 0.1 mL Tube and Cap Strips for Qiagen Rotor-Gene Q 
Real-Time Rotary Analyzer

T319-72D2

Rotocycler 72 Rotor with 100 µL wells for Qiagen Rotor-Gene Q 
Real-Time Rotary Analyzer

T319-100D1

Rotocycler 100 Rotor with 25 µL wells for Qiagen Rotor-Gene Q 
Real-Time Rotary Analyzer
Item#:
T319-4WS1
Your Price:
536.33
Each
500 Pre-Scored Sheets

FoilSeal™ RollMate™ Dispenser +1 Roll of FoilSeal™ Film, 500 Pre-Scored Sheets

Pre-scored film on a roll is a more economical and efficient alternative to rapidly seal microplates; faster than applying single sheeted film. Self-standing, non-slip supports hold the pre-scored film roll securely in place for quick and easy bench-top dispensement. The roll unwind tension can be easily adjusted to user preference. Continuous backing liner can be torn off or fed directly into a wastebasket for disposal.
  • Certified DNase- & RNase- free
  • Pierceable with a pipet tip or robotic probe for direct sample recovery
  • 2.0mil aluminum foil removes easily without tearing
  • The conformable nature of foil allows for the visual verification of the seal’s security around each well
  • Not recommended for long-term cold storage
Applications
  • PCR
  • Light sensitive assays
  • Short-term storage
  • Robotics
Temperature Range
  • -20°C to +120°C
Helpful Tips
  • End-tab removal before thermal cycling is highly recommended
  • Remove diagonally from a corner to prevent tearing

Item#:
ASFOILSEALROLLMATE
 
FoilSeal RollMate Features
Specs FoilSeal RollMate
Sealing film selection guide by application
Sealing film selection guide by attributes
Soft, Extra Thick, Conformable Adhesive

ThermalSeal® 2™ and ThermalSeal® Advanced Sealing Film

ThermalSeal® 2™ and ThermalSeal® Advanced feature a soft, extra thick, conformable adhesive that forms a very strong seal around raised wells to minimize evaporation and cross-contamination.
  • Certified DNase- & RNase- free
  • Ideal for use with flat-top, semi-skirted and full-skirted PCR plates
  • Compatible with hard-shell dual component (e.g. polycarbonate/polypropylene) PCR plates
  • Large cut allows for easier positioning of the film to the plate via edge of plate alignment vs. centering
  • Available in a smaller cut for raised-rim and non-skirted PCR plates: ThermalSeal® Advanced
Application
  • PCR
Temperature Range
  • -40°C to +120°C
Helpful Tips
  • Selection of the proper film size will minimize evaporation
  • The film should not overhang or ride up the wall of a raised-rim plate
  • More complete coverage of skirted flat-top plates will reduce evaporation in the outer wells
  • End-tab removal before thermal cycling is highly recommended

Item#:
ASTHERMSEAL2A
 
Specs ThermalSeal 2
Sealing film selection guide by application
Sealing film selection guide by attributes
ThermalSeal 2 Features
Black Vinyl Sealing Film for Black Microplates

AbsorbMax™ Black Vinyl Sealing Film for Black Microplates

AbsorbMax™ is a black vinyl film that can be applied to either the top or bottom of the plate.

  • Minimizes background fluorescence and cross talk between plate wells
  • Ideal as a back seal for black plates with clear well bottoms for top reading measurements
  • Absorbs stray light, reducing the background caused by light scatter
  • Protects light sensitive samples from degradation
  • Features a single split liner for easy application

Applications

  • Fluorescence assays
  • Light sensitive sample protection

Temperature Range

  • -40°C to +80°C
Item#:
ASABSORBMAX
 
AbsorbMax Features
Specs AbsorbMax
Sealing film selection guide by application
Sealing film selection guide by attributes

Zone-Free™


Zone-Free is a multilayer film consisting of a top pierceable clear polyethylene film and a white polypropylene sublayer with 96 circular areas void of adhesive.

Application: Short-term storage and robotics
Temperature Range: -40°C to +90°C

The white sublayer aids in the alignment of the film to the plate; alignment of opposing corner wells ensures that all 96 wells will be properly aligned. The adhesive-free zones are 9mm on center to facilitate multi-channel pipetting without adhesive fouling of pipet tips or robotic probes.

  • Pierceable
  • Offers excellent chemical resistance
  • Samples have minimal contact with adhesive due to the adhesive-free zones
Item#:
ASZONEFREFIL
 
Sealing film selection guide by application
Sealing film selection guide by attributes
Zone-FreeFilm Features
Specs Zone-FreeFilm
Format, 24/cs, 100uL tubes for

The RotoCycler Discs are specially made to be used with the Qiagen Rotor-Gene Q Real-Time Rotary Analyzer. Two models are available: a 72-well format with 100 µL tubes and a 100-well format for reactions up to 25 µL. The discs a"one-piece "plate" equivalent, having vertically oriented wells compatible with automated reaction setup using a robotic liquid handling system.

Item#:
T319-72D2
Your Price:
226.40
Each
Item#:
ASTHINSEALSFI
1mL, 1 vial of 1000ul

Affordable price & shipped at room temperature.

 

A premixed, ready-to-use solution for efficient amplification of DNA templates by PCR.

  • 2X Eco-Taq MasterMix contains Taq DNA Polymerase, dNTPs, Mg2+ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR, as well as an inert loading dye.
  • This pre-mixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set up. To prepare the final PCR, only primers and template DNA need to be added.
  • The mix retains all features of Taq DNA Polymerase and can amplify DNA targets up to 5 kb (simple template).
  • The elongation velocity is 0.9~1.2kb/min (70~75°C).
  • It has 5' to 3' polymerase activity but lacks 3' to 5' exonuclease activity which results in a 3'-dA overhang PCR product.

 

Features:

  • Convenient: Just add primers and template DNA
  • High yields of PCR products with minimal optimization.
  • High efficiency: saves your time by simplifying the process
  • Reproducible: lower contamination risk and pipetting error.

    Applications:

  • High-throughput PCR.
  • Routine PCR with high reproducibility
  • Generation of PCR products for TA cloning

    Contents:
    2X Taq Mix 1ml

    Store at -20°C
    For research use only

Item#:
ECO-TAQ1
Your Price:
35.00
Each

MIDSCI ELISA & Short Term


The polyester films are a great general purpose seal; ideal for sample protection and elimination of well-to-well sample cross contamination. The acrylic adhesive offers a strong bond that seals tightly on polypropylene and polystyrene plates.

This seal is ideally suited for short term storage, Elisa assays, prevention of sample loss, and general lab usage.

The paper backing of this film is scored on both ends to allow for two handed (precise) placement on the plate.

For PCR applications see our standard PCR film or our Real Time PCR film.

For applications where accessing samples through the seal with pipet tips or a probe is required, see the pierceable aluminum (MID-PCR-200) film.

Certified free of RNase, DNase, Pyrogen, and nucleic-acid contamination.
 

Specifications: 

Length146.05 mm
Length (w/ end tabs removed)119.05 mm
Width79.38 mm
Overall Thickness3.1 mil
Adhesive Thickness1.1 mil
MaterialPolyester
AdhesiveMedical Grade Acrylic
Max. Temperature120 °C
Min. Temperature-40 °C
Primary UsagesElisa, Storage or Sample Cover
 
Item#:
ASMIDSCIELISA
Item#:
ASSEALPOLY3P9
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