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PCR/qPCR

MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more.  If you are looking for any of these things, we can match almost all needs to all cyclers.  If you don’t find it easily contact us at tech@midsci.com and we will find it for you. 

96 or 384 we have it.  Non-Skirted, semi-skirted or full skirted, we have it.  0.1, 0.2, or 0.5 mL we have it.  A1, A12, H12, A24, P24 cuts, we have them.  Sybr© Green or probe alternative, we have it.  

Pro Tip: Colored plastics do not have any impact on DNA amplification.  But when setting up qPCR it is often recommended to use white plastics.  This will limit or prevent fluorescence refraction.  Light refraction can minimize sensitivity and consistency. 

Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips.  This will help optimize and reduce variance in the reactions.  Simple test, see the residual liquid left in tips?  This means some of the taq, dNTPs, sample, etc is left in the tip.  PR1MA tips help reduce that significantly and improve your consistency. 

Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate?  Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler.  Call us we have solutions.

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Pryme Ergonomic PCR Strip Tubes and caps

Pryme Ergonomic PCR 8-Strip Tubes & Caps on this page are interchangeable.  All strip tubes work with all caps.  Please also know there is a list of plates below that the strip caps will work with.  All plastics on this page are certified DNA, DNase, RNA, RNase and pyrogen-free.

Tube & Cap Characteristics

  • Easy open - easy close
  • Available 0.1 & 0.2 mL
  • Extra robust construction for more stable shape and preventing breakage
  • Significant reduction in evaporation due to a wider sealing area
  • Extreme Uniformity (EU) Tube Design
    • Uniform wall thickness ensures consistent even heating
    • Thin wall-bottomed tube for rapid heat transfer
  • Optical clear caps great for qPCR
  • Extra robust for easy handling and easier to open and close
    • Certified DNA, DNase, RNA, RNase and pyrogen-free

Strip Caps are compatible with these plates from MIDSCI

Order #

Plate Description

AB70651

Pryme PCR Plate, 96-Well, 0.1 mL, Non-Skirt, Low Profile, Frosted, 25/cs

AB70659

Pryme PCR Plate, 96-Well, 0.1 mL, Non-Skirt, Low Profile, White, 25/cs

B70651

Pryme PCR Plate, 96-Well, 0.1 mL, Non-Skirt, Cuttable, Regular Profile, Natural, 25/cs

B70659

Pryme PCR Plate, 96-Well, 0.1 mL, Non-Skirt, Cuttable, Regular Profile, White, 25/cs

B17480

Pryme PCR Plate, 96-Well, 0.1 mL, Semi-skirted, Low Profile, Frosted, 25/cs

B17489

Pryme PCR Plate, 96-Well, 0.1 mL, Semi-skirted, Low Profile, White, 25/cs

AB19800-EU

Pryme PCR Plate, 96-Well, 0.1 mL, Sub-skirted, Low Profile, Frosted, 25/cs

AB19809-EU

Pryme PCR Plate, 96-Well, 0.1 mL, Sub-skirted, Low Profile, White, 25/cs

B70501

Pryme PCR Plate, 96-Well, 0.2 mL, Non-Skirt, Regular Profile, Natural, 25/cs

B70509

Pryme PCR Plate, 96-Well, 0.2 mL, Non-Skirt, Regular Profile, White, 25/cs

B50501

Pryme PCR Plate, 96-Well, 0.2 mL, Non-Skirt, Cuttable, Regular Profile, Frosted, 25/cs

B50509

Pryme PCR Plate, 96-Well, 0.2 mL, Non-Skirt, Cuttable, Regular Profile, White, 25/cs

B50651

Pryme PCR Plate, 96-Well, 0.2 mL, Semi-skirted, Cuttable, Regular Profile, Frosted, 25/cs

B50659

Pryme PCR Plate, 96-Well, 0.2 mL, Semi-skirted, Cuttable, Regular Profile, White, 25/cs

ABI7501

Pryme PCR Plate, 96-Well, 0.2 mL, Sub-skirted, Regular Profile, Frosted, 25/cs

ABI7509

Pryme PCR Plate, 96-Well, 0.2 mL, Sub-skirted, Regular Profile, White, 25/cs
Item#:
ASPCRTUBES9
Item#:
ASKF24V
ABI, 10/pk

Please know that the manufacturer has suspended manufacturing this product indefinitely. 
We suggest purchasing PR1MA PCR Plastics.
Contact your rep or email tech@midsci.com for more information.

  • Standard thermal cycling and qPCR compatible
  • Ultra-thin and consistent wall thickness for precise thermal transfer
  • Made from 99.9% virgin polypropylene
  • Single notch corner
  • 335ul well capacity (working capacity dependent on closure method)
  • Fits standard thermal cyclers, ABI Real Time PCR 7300, 7500, 7900, qPCR and ABI Sequencing
  • Certified DNAse/RNAse and pyrogen-safe
Item#:
PCR-96-AB-C
Your Price:
76.17
Each
96x0.1ml, Sub-Skirted,

Designed to fit ABI 9800 and other fast cycler sample blocks
  • Thin-wall for consistent heat transfer
  • Ultra-low sample evaporation
  • Certified DNAse/RNAse, nucleic acid, PCR-inhibitor, and pyrogen-FREE
  • Fits the following ABI Cyclers: 7500 Fast, 7900 Fast, 9800 Fast, StepOne Plus, Veriti Fast

Closure can be accomplished with B57801 optical indented strip caps.
Item#:
AB19807-EU
Your Price:
158.24
Each
This item is discontinued
Tubes Only, 0.2mL, Ex. Robust,

    Pryme Ergonomic PCR 8-Strip Tubes

      • Tube Characteristics
        • 0.2mL, regular profile
        • Easy open - easy close
        • Extra robust construction for more stable shape and preventing breakage
      • Extreme Uniformity (EU) Tube Design
        • Uniform wall thickness ensures consistent even heating
        • Thin wall-bottomed tube for rapid heat transfer
      • Pairs with EU âSealing Ring Band❠designed caps
        • Caps are significantly easier to apply and remove -- Repetitive Strain Injury (RSI) Friendly.
        • Significant reduction in evaporation due to a wider sealing area.
      • Certified DNA, DNase, RNA, RNase and pyrogen-free

    Order #

    Description

    B69901

    Pryme PCR Ergonomic 8-Strip Tubes, 0.2mL, Extra Robust, Frosted, 120/cs

    B69909

    Pryme PCR Ergonomic 8-Strip Tubes, 0.2mL, Extra Robust, White, 120/cs

    B57801

    Pryme PCR Ergonomic 8-Strip, Optical Flat (V2) Caps, Natural, 120/cs

    B79701

    Pryme PCR Ergonomic 8-Strip, Optical Flat (V1) Caps, Natural, 120/cs

    C79701

    Pryme PCR Ergonomic 8-Strip, Semi-Domed (V1) Caps, Natural, 120/cs
Item#:
B69901
Your Price:
149.45
Each

PCR Cooler, 96 Well


Ice-Free Cooling System for PCR Preparation and Storage

  • Modern design cools faster in freezers and stays cold longer on the bench top
  • Maintains the entire 96-well plate at 0C for over an hour
  • Maintains sample temperature of less than 5C for up to three hours (with lid off)
  • When the temperature ceiling of 6C has been exceeded there is a contrasting color change of the cooler or a single well
  • Cools industry standard format PCR tubes, strips and single format as all as most 96-well plates in both standard and low profile well shape
  • Patented lid design provides seamless storage and transport by securely covering the PCR samples from the freezer to the bench top
  • Innovative lid slides out, rotates 180 degrees, and becomes a convenient stand the cooler sits on
  • Well design prohibits the freezing of samples
  • Compact space saving design, stackable
  • Cooler made from engineered polypropylene and lid is made from polycarbonate
  • Two-year warranty 
Item#:
ASPCRCOOLER96
Tubes Only, 0.2mL, Ex. Robust,


    Pryme Ergonomic PCR 8-Strip Tubes

      • Tube Characteristics
        • 0.2mL, regular profile
        • Easy open - easy close
        • Extra robust construction for more stable shape and preventing breakage
      • Extreme Uniformity (EU) Tube Design
        • Uniform wall thickness ensures consistent even heating
        • Thin wall-bottomed tube for rapid heat transfer
      • Pairs with EU Sealing Ring Band designed caps
        • Caps are significantly easier to apply and remove -- Repetitive Strain Injury (RSI) Friendly.
        • Significant reduction in evaporation due to a wider sealing area.
      • Certified DNA, DNase, RNA, RNase and pyrogen-free

    Order #

    Description

    B69901

    Pryme PCR Ergonomic 8-Strip Tubes, 0.2mL, Extra Robust, Frosted, 120/cs

    B69909

    Pryme PCR Ergonomic 8-Strip Tubes, 0.2mL, Extra Robust, White, 120/cs

    B57801

    Pryme PCR Ergonomic 8-Strip, Optical Flat (V2) Caps, Natural, 120/cs

    B79701

    Pryme PCR Ergonomic 8-Strip, Optical Flat (V1) Caps, Natural, 120/cs

    C79701

    Pryme PCR Ergonomic 8-Strip, Semi-Domed (V1) Caps, Natural, 120/cs
Item#:
B69909
Your Price:
149.45
Each
96x0.2ml, Sub-Skirted,
  • Plate Characteristics
    • 96 x 0.1mL, Sub-skirted
    • Low Profile (0.1mL), Frosted (low signal to noise ratio
    • Extreme Uniformity (EU) Plate Design
    • Uniform wall thickness ensures consistent even heating
    • Thin wall-bottomed tube for rapid heat transfer
  • Ideal paring with EU Caps with "Sealing Ring Band" Design
    • Easy open - easy close with ergonomic caps: B57801, B79701, C79701
    • Caps are significantly easier to apply and remove -- Repetitive Strain Injury (RSI) Friendly.
    • Significant reduction in evaporation due to a wider sealing area.
  • Certified DNA, DNase, RNA, RNase and pyrogen-free

Please give us a call for a sample!

 

Order#

Description

Non-Skirt

 

B70501

Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, Natural, 25/cs

B70509

Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, White, 25/cs

B50501

Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, Frosted, 25/cs

AB70651

Pryme PCR Plate, 96-Well, 0.1mL, Non-Skirt, Low Profile, Frosted, 25/cs

Semi-Skirted

 

B70651

Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, Natural, 25/cs

B70659

Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, White, 25/cs

B50651

Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, Frosted, 25/cs

B17480

Pryme PCR Plate, 96-Well, 0.1mL, Semi-skirted, Low Profile, Frosted, 25/cs

B17489

Pryme PCR Plate, 96-Well, 0.1mL, Semi-skirted, Low Profile, White, 25/cs

Sub-Skirted

 

ABI7501

Pryme PCR Plate, 96-Well, 0.2mL, Sub-skirted, Regular Profile, Frosted, 25/cs

ABI7509

Pryme PCR Plate, 96-Well, 0.2mL, Sub-skirted, Regular Profile, White, 25/cs

AB19800-EU

Pryme PCR Plate, 96-Well, 0.1mL, Sub-skirted, Low Profile, Frosted, 25/cs

AB19809-EU

Pryme PCR Plate, 96-Well, 0.1mL, Sub-skirted, Low Profile, White, 25/cs

Caps

 

B57801

Pryme PCR Ergonomic 8-Strip, Optical Flat (V2) Caps, Natural, 120/cs

B79701

Pryme PCR Ergonomic 8-Strip, Optical Flat (V1) Caps, Natural, 120/cs

C79701

Pryme PCR Ergonomic 8-Strip, Semi-Domed (V1) Caps, Natural, 120/cs
Item#:
ABI7509
Your Price:
171.43
Each
Tubes with Domed Strip Caps
This item has been discontinued and replaced by item 
Please know that the manufacturer has suspended manufacturing this product indefinitely. 
We suggest purchasing PR1MA PCR Plastics.
Contact your rep or email tech@midsci.com for more information.

 
  • Ultra-thin and consistent wall thickness for precise thermal transfer
  • Full-length insertion cap design prevents sample evaporation
  • Made of 99.9% pure virgin polypropylene
  • Optical flat cap versions feature ultra-clear flat caps, ideal for real-time PCR, fluorescence and luminescence assays; offer light transmittance that is 75% higher than with standard strip caps
  • Certified DNAse/RNAse, and pyrogen-free
Item#:
PCR-0208-CP-C
This item is discontinued
96x0.2ml, Sub-Skirted,

Plate Characteristics

    • 96 x 0.1mL, Sub-skirted
    • Low Profile (0.1mL), Frosted (low signal to noise ratio
    • Extreme Uniformity (EU) Plate Design
    • Uniform wall thickness ensures consistent even heating
    • Thin wall-bottomed tube for rapid heat transfer
  • Ideal paring with EU Caps with "Sealing Ring Band" Design
    • Easy open - easy close with ergonomic caps: B57801, B79701, C79701
    • Caps are significantly easier to apply and remove -- Repetitive Strain Injury (RSI) Friendly.
    • Significant reduction in evaporation due to a wider sealing area.
  • Certified DNA, DNase, RNA, RNase and pyrogen-free

Please give us a call for a sample!

 

Order#

Description

Non-Skirt

 

B70501

Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, Natural, 25/cs

B70509

Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, White, 25/cs

B50501

Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, Frosted, 25/cs

AB70651

Pryme PCR Plate, 96-Well, 0.1mL, Non-Skirt, Low Profile, Frosted, 25/cs

Semi-Skirted

 

B70651

Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, Natural, 25/cs

B70659

Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, White, 25/cs

B50651

Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, Frosted, 25/cs

B17480

Pryme PCR Plate, 96-Well, 0.1mL, Semi-skirted, Low Profile, Frosted, 25/cs

B17489

Pryme PCR Plate, 96-Well, 0.1mL, Semi-skirted, Low Profile, White, 25/cs

Sub-Skirted

 

ABI7501

Pryme PCR Plate, 96-Well, 0.2mL, Sub-skirted, Regular Profile, Frosted, 25/cs

ABI7509

Pryme PCR Plate, 96-Well, 0.2mL, Sub-skirted, Regular Profile, White, 25/cs

AB19800-EU

Pryme PCR Plate, 96-Well, 0.1mL, Sub-skirted, Low Profile, Frosted, 25/cs

AB19809-EU

Pryme PCR Plate, 96-Well, 0.1mL, Sub-skirted, Low Profile, White, 25/cs

Caps

 

B57801

Pryme PCR Ergonomic 8-Strip, Optical Flat (V2) Caps, Natural, 120/cs

B79701

Pryme PCR Ergonomic 8-Strip, Optical Flat (V1) Caps, Natural, 120/cs

C79701

Pryme PCR Ergonomic 8-Strip, Semi-Domed (V1) Caps, Natural, 120/cs
Item#:
ABI7501
Your Price:
180.22
Each
96-Well, 50/pk

Permanently label mutliple microplates with clear Well Orienter Stickers. Each well is clearly marked with its corresponding row number and column letter for easy cross-referencing. The paper backing is scored in the middle allowing for easy aligning on the underside of the plate.

U of M: 50/pack

Layout: 96-well

Description: Made of clear polyester with acrylic adhesive

Item#:
WEST-1000
Your Price:
34.50
Each
Item#:
AS384V200UL
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