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PCR/qPCR

MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more.  If you are looking for any of these things, we can match almost all needs to all cyclers.  If you don’t find it easily contact us at tech@midsci.com and we will find it for you. 

96 or 384 we have it.  Non-Skirted, semi-skirted or full skirted, we have it.  0.1, 0.2, or 0.5 mL we have it.  A1, A12, H12, A24, P24 cuts, we have them.  Sybr© Green or probe alternative, we have it.  

Pro Tip: Colored plastics do not have any impact on DNA amplification.  But when setting up qPCR it is often recommended to use white plastics.  This will limit or prevent fluorescence refraction.  Light refraction can minimize sensitivity and consistency. 

Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips.  This will help optimize and reduce variance in the reactions.  Simple test, see the residual liquid left in tips?  This means some of the taq, dNTPs, sample, etc is left in the tip.  PR1MA tips help reduce that significantly and improve your consistency. 

Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate?  Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler.  Call us we have solutions.

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Adjustable Well Plate Stand

Achieves accurate pipetting by allowing the flexibility to adjust the angle of the microplate (45° max. tilt). The stand takes up little benchtop space measuring only 6 x 4.5 x 2.5 In. (D x W x H).

*Well Orienters are sold separately.
 

Proudly made in the USA! Click here for more made in America products!
 

Item#:
ASWELLPLATESTAND
Limited Time Promo!


2025 LabValues Promotion!
For a limited time enjoy discounted pricing on the
ENDURO™ Power Supply Series (E0303 and E0203)!

 

See flyer for more details.

 

Special price applies only to the specified item; no substitutions.

Cannot be combined with other discounts or pricing. Not valid on prior purchases.

Promo is valid 01/06/2025 – 06/30/2025 or while supplies last.

All terms and conditions apply.


ENDURO™ Power Supply 

  • Two models (300V and 250V) available
  • For DNA, RNA, or protein electrophoresis, and blotting applications
  • Constant voltage or constant current with automatic crossover
  • 250 V model also offers a constant power operating mode
  • Timed or continuous operation
  • Short circuit and over current/ over voltage protection
  • No load and load change detection system
  • Programming includes option for automatic recovery after power failure
  • 3 year warranty

The new Enduro™ Power Supplies meet a wide range of applications including DNA, RNA, and protein electrophoresis, blotting, and many others. Two models are available with voltage ranges to 300 V and current ranges to 3000 mA. Operating modes include constant voltage or constant current with automatic crossover. In addition, the 250V model offers a constant power operating mode.

All parameters, including voltage, current, power (250 V model only), and time are easily set using the membrane keypad and displayed digitally on the back lit LCD. Compact in design with a small footprint, the Enduro™ Power Supplies are also stackable to save space. For easy viewing and setting of parameters on the bench or on a shelf, the front of the power supply can be raised with the integral folding feet.

Specifications

Model300V250V
Output Voltage Range/Increments2-300V/1V5-250V/1V
Output Current Range/Increments4-500mA/1mA10-3000mA/10mA
Output Power Range/Increments90 watts max/n.a.1-300W/1W
Operating Constant Modescurrent or voltagecurrent, voltage, or power
Timer1 -99 min, or continuous1min. to 99h 59min or continuous
ProgrammablenoYes (20 program capacity)
Dimensions (W x D x H)19 x 25 x 8 cm19 x 25 x 8 cm
Weight2.6 lbs5.7 lbs
Input Voltage110-230 V110-230 V
Item#:
ASPOWERSUP1


Multi-colored 96 Well Orienter

 

For easy identification of samples in microplates, vertically or horizontally oriented bright colors make it easy to distinguish between columns. Fits most standard flat and round bottom 96-well plates. Available in horizontal and vertical orientations.
 

Proudly made in the USA! Click here for more made in America products!

Item#:
ASCHROMPLACC2
Limited Time Promo!


2025 LabValues Promotion!
For a limited time enjoy discounted pricing on select
Labnet Enduro™ UV Transilluminators (U1001 and U1002)!

 

See flyer for more details.

 

Special price applies only to the specified item; no substitutions.

Cannot be combined with other discounts or pricing. Not valid on prior purchases.

Promo is valid 01/06/2025 – 06/30/2025 or while supplies last.

All terms and conditions apply.


Enduro™ UV Transilluminators


The NEW ENDURO™ UV Transilluminator represents a great new imaging product true to ENDURO™ name. This UV transilluminator has been designed for rugged use and durability with exceptional performance.
  • Compact size with 21 x 26 cm viewing area
  • Available in single or dual wavelength models
  • Unique heavy duty hinge design can be held in place at any angle for gel cutting
  • UV shield fully protects user from UV light
  • Uniform UV Black Glass provides improved imaging contrast
 
The use of UV Black Glass minimizes the striping effect seen on many UV transilluminators. This provides a more uniform background for gel imaging.
Item#:
ASENDUROUVTRANS
96x0.1ml, Sub-Skirted,
This item has been discontinued and replaced by item 
  • Plate Characteristics
    • 96 x 0.1mL, Sub-skirted
    • Low Profile (0.1mL), Frosted (low signal to noise ratio
    • Extreme Uniformity (EU) Plate Design
    • Uniform wall thickness ensures consistent even heating
    • Thin wall-bottomed tube for rapid heat transfer
  • Ideal paring with EU Caps with "Sealing Ring Band" Design
    • Easy open - easy close with ergonomic caps: B57801, B79701, C79701
    • Caps are significantly easier to apply and remove -- Repetitive Strain Injury (RSI) Friendly.
    • Significant reduction in evaporation due to a wider sealing area.
  • Certified DNA, DNase, RNA, RNase and pyrogen-free

Please give us a call for a sample!

 

Order#

Description

Non-Skirt

 

B70501

Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, Natural, 25/cs

B70509

Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, White, 25/cs

B50501

Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, Frosted, 25/cs

AB70651

Pryme PCR Plate, 96-Well, 0.1mL, Non-Skirt, Low Profile, Frosted, 25/cs

Semi-Skirted

 

B70651

Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, Natural, 25/cs

B70659

Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, White, 25/cs

B50651

Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, Frosted, 25/cs

B17480

Pryme PCR Plate, 96-Well, 0.1mL, Semi-skirted, Low Profile, Frosted, 25/cs

B17489

Pryme PCR Plate, 96-Well, 0.1mL, Semi-skirted, Low Profile, White, 25/cs

Sub-Skirted

 

ABI7501

Pryme PCR Plate, 96-Well, 0.2mL, Sub-skirted, Regular Profile, Frosted, 25/cs

ABI7509

Pryme PCR Plate, 96-Well, 0.2mL, Sub-skirted, Regular Profile, White, 25/cs

AB19800-EU

Pryme PCR Plate, 96-Well, 0.1mL, Sub-skirted, Low Profile, Frosted, 25/cs

AB19809-EU

Pryme PCR Plate, 96-Well, 0.1mL, Sub-skirted, Low Profile, White, 25/cs

Caps

 

B57801

Pryme PCR Ergonomic 8-Strip, Optical Flat (V2) Caps, Natural, 120/cs

B79701

Pryme PCR Ergonomic 8-Strip, Optical Flat (V1) Caps, Natural, 120/cs

C79701

Pryme PCR Ergonomic 8-Strip, Semi-Domed (V1) Caps, Natural, 120/cs
Item#:
AB19809-EU
Your Price:
158.24
Each
This item is discontinued
Item#:
ASPCR384FULS
Skirt, PCR, Clear, 10/pk
This item has been discontinued and replaced by item 
Please know that the manufacturer has suspended manufacturing this product indefinitely.
We suggest purchasing PR1MA PCR Plastics.
Contact your rep or email tech@midsci.com for more information.


 
  • Standard thermal cycling and qPCR compatible
  • Ultra-thin and consistent wall thickness for precise thermal transfer
  • Automation friendly, single notch corner, stackable
  • Made from 99.9% virgin polypropylene
  • 240ul well capacity (working capacity dependent on closure method)
  • Barcoding available
  • Certified DNAse/RNAse and pyrogen-safe
Item#:
PCR-96-FS-C
Your Price:
60.39
Each
This item is discontinued
Amplification Plate
Please know that the manufacturer has suspended manufacturing this product indefinitely. 
We suggest purchasing PR1MA PCR Plastics.
Contact your rep or email tech@midsci.com for more information.
 

  • Standard thermal cycling and qPCR compatible
  • Ultra-thin and consistent wall thickness for precise thermal transfer
  • Single notch corner
  • Made from 99.9% virgin polypropylene
  • 340ul well capacity (working capacity dependent on closure method)
  • Fits standard thermal cyclers, plus ABI PCR 2700, 2720, 9700, 9800, 7300, 7500, 7900, and ABI sequencing units
  • Certified DNAse/RNAse and pyrogen-safe
Item#:
PCR-96M2-HS-C
Your Price:
42.96
Each
This item is discontinued
Item#:
ASPCR96ELEV

PRYME PCR plastics are designed and optimized for PCR & qPCR applications.

Unique design and development provides maximum mold and manufacturing accuracy. We outline precise product specifications and tolerances and minimize mold cavities while using CYCLERtest's temperature control competence in our manufacturing process. Result: superior and reproducible products with, in case of (q)PCR vessels, an average wall thickness of 0.30 mm for tubes and strips and 0.35 mm for plates, both with a maximum tolerance of 0.05 mm. Combined with the identical design of (q)PCR product closure one ends up in fully interchangeable and freely selectable (q)PCR cap and vessel combinations.

Extreme uniform wall thickness leads to a very even heating of the sample and more homogeneous reaction conditions, leading to more reproducible results. Extreme uniform wall thickness also leads to lower evaporation rates and therefore more consistent (q)PCR results. Evaporation rates of >10% up to 30% significantly influence the ratio of your (q)PCR components in the vessel, and thus the resulting reaction and its result. In case of optical detection via optical reading through the side of the qPCR tubes, better wall uniformity increases the signal consistency.

Clever design and sealing bands around the tube closure points provide a leak-free seal, reducing evaporation and leading to more reproducible results. These superior sealing properties allow for running (q)PCR reactions with volumes as low as 5 µl. The cap design allows minimum pressure for closure and opening which is highly appreciated by users and minimizes RSI risks.


Item#:
ASPRYMEERGPCRTUBE
Item#:
ASPCR96N0P1W
Please know that the manufacturer has suspended manufacturing this product indefinitely. 
We suggest purchasing PR1MA PCR Plastics.
Contact your rep or email tech@midsci.com for more information.

 PCR Fast Plate, 96x0.1ml

  • Optimized for fast PCR and qPCR compatible
  • Ultra-thin and consistent wall thickness for precise thermal transfer
  • Made from 99.9% virgin polypropylene
  • 240ul well capacity (working capacity dependent on closure method)
  • Designed for ABI thermal cyclers 7500 and 7900, 9800, Veriti, and StepOne
  • Certified DNAse/RNAse and pyrogen-safe
Item#:
ASPRCFASTPLATE
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