S2K Commerce - Products Dropdown
S2K Commerce - Shopping Cart
S2K Commerce - Order Entry
PCR/qPCR
MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more. If you are looking for any of these things, we can match almost all needs to all cyclers. If you don’t find it easily contact us at tech@midsci.com and we will find it for you.
96 or 384 we have it. Non-Skirted, semi-skirted or full skirted, we have it. 0.1, 0.2, or 0.5 mL we have it. A1, A12, H12, A24, P24 cuts, we have them. Sybr© Green or probe alternative, we have it.
Pro Tip: Colored plastics do not have any impact on DNA amplification. But when setting up qPCR it is often recommended to use white plastics. This will limit or prevent fluorescence refraction. Light refraction can minimize sensitivity and consistency.
Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips. This will help optimize and reduce variance in the reactions. Simple test, see the residual liquid left in tips? This means some of the taq, dNTPs, sample, etc is left in the tip. PR1MA tips help reduce that significantly and improve your consistency.
Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate? Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler. Call us we have solutions.
PurePlus® PCR Plates
- PurePlus® plates are tested for RNase/DNase lot by lot to ensure pure product
- Our plates are lot certified free of Human DNA contamination
- Our plates are tested for ATP lot by lot to ensure pure consistent product
- Broad product range. Over 18 styles of plates fit most major thermocyclers
- These plates are autoclavable to 122° C for 15 minutes at 15PSI
Ultra Clear and Ultra Clean
PurePlus® Polymerase Chain Reaction (PCR) consumables are made without additives so you don't need to wonder what is different about the plates, tubes, or strips you are using this week. We also test every batch for contamination by Human DNA as well as the more common tests that other suppliers offer for RNase and DNase enzymes. Many of our plates are printed with black alpha numeric reference grids to make it easier for you to keep track of your samples. We also offer optically clear caps and opaque black or white plates for Real Time PCR. And we also offer re-usable silicone sealing mats and polypropylene sealing film. Labcon PurePlus® PCR tubes, strips and plates are designed to fit many popular thermocyclers including brands like Applied Biosystems, Eppendorf, Thermo Scientific, and Bio Rad. All our PCR disposables are made from 100% virgin polypropylene and are lot tested and certified to be free of RNase, DNase, and Human DNA. Autoclavable. Our pure resin consumables are available in strips, individual tubes, plates in clear natural or colors.PurePlus® sterile disposables, like all our other sterile products, receive valid sterilization processing compliant with ISO 11137. So unlike "pre-sterile" products from other suppliers you can use PurePlus® PCR disposables for diagnostic and regulated procedures.
Proudly made in the USA! Click here for more made in America products!
Adjustable Well Plate Stand
Achieves accurate pipetting by allowing the flexibility to adjust the angle of the microplate (45° max. tilt). The stand takes up little benchtop space measuring only 6 x 4.5 x 2.5 In. (D x W x H).
*Well Orienters are sold separately.Proudly made in the USA! Click here for more made in America products!
LabValues 2025 Promo!
For a limited time, enjoy special pricing on select Labnet Enduro™ Power Supplies!
(E0303, E0203)
Offer cannot be combined with other discounts or institutional pricing.
Offer not valid on previous purchases. Promo valid from 7/15/25 to 12/31/25 or while supplies last.
All terms and conditions apply.
ENDURO™ Power Supply
- Two models (300V and 250V) available
- For DNA, RNA, or protein electrophoresis, and blotting applications
- Constant voltage or constant current with automatic crossover
- 250 V model also offers a constant power operating mode
- Timed or continuous operation
- Short circuit and over current/ over voltage protection
- No load and load change detection system
- Programming includes option for automatic recovery after power failure
- 3 year warranty
The new Enduro™ Power Supplies meet a wide range of applications including DNA, RNA, and protein electrophoresis, blotting, and many others. Two models are available with voltage ranges to 300 V and current ranges to 3000 mA. Operating modes include constant voltage or constant current with automatic crossover. In addition, the 250V model offers a constant power operating mode.
All parameters, including voltage, current, power (250 V model only), and time are easily set using the membrane keypad and displayed digitally on the back lit LCD. Compact in design with a small footprint, the Enduro™ Power Supplies are also stackable to save space. For easy viewing and setting of parameters on the bench or on a shelf, the front of the power supply can be raised with the integral folding feet.
Specifications
Model | 300V | 250V |
Output Voltage Range/Increments | 2-300V/1V | 5-250V/1V |
Output Current Range/Increments | 4-500mA/1mA | 10-3000mA/10mA |
Output Power Range/Increments | 90 watts max/n.a. | 1-300W/1W |
Operating Constant Modes | current or voltage | current, voltage, or power |
Timer | 1 -99 min, or continuous | 1min. to 99h 59min or continuous |
Programmable | no | Yes (20 program capacity) |
Dimensions (W x D x H) | 19 x 25 x 8 cm | 19 x 25 x 8 cm |
Weight | 2.6 lbs | 5.7 lbs |
Input Voltage | 110-230 V | 110-230 V |
Multi-colored 96 Well Orienter
For easy identification of samples in microplates, vertically or horizontally oriented bright colors make it easy to distinguish between columns. Fits most standard flat and round bottom 96-well plates. Available in horizontal and vertical orientations.
Proudly made in the USA! Click here for more made in America products!
LabValues 2025 Promo!
For a limited time, enjoy special pricing on Labnet Enduro™ UV Transilluminators!
Offer cannot be combined with other discounts or institutional pricing.
Offer not valid on previous purchases. Promo valid from 7/15/25 to 12/31/25 or while supplies last.
All terms and conditions apply.
- Compact size with 21 x 26 cm viewing area
- Available in single or dual wavelength models
- Unique heavy duty hinge design can be held in place at any angle for gel cutting
- UV shield fully protects user from UV light
- Uniform UV Black Glass provides improved imaging contrast
This brand is being discontinued and will only be available while supplies last.
Need a great DNA Safe Stain at a great price?
Try PR1MA! Click here to order.
Bullseye DNA SafeStain,10,000x
- Safest DNA stain by far
- Replaces EthBr in agarose gel electrophoresis
- Detection of DNA and RNA
- Excitation at 290 nm & 490 nm
- Emission at 530 nm
DNA SafeStain is a new and safe nucleic acid stain for visualization of double-stranded DNA, single-stranded DNA, and RNA in agarose gels. The dyes are developed to replace toxic ethidium bromide (EthBr, a potent mutagen), commonly used in gel electrophoresis for visualization of nucleic acids in agarose gels. DNA SafeStain is non-carcinogenic by the Ames-test. The results are negative in both the mouse marrow chromophilous erthrocyte micronucleus and mouse spermary spermatocyte chromosomal aberration tests.
DNA SafeStain emits green fluorescence when bound to DNA. It has two excitation wavelength peaks when bound to nucleic acid, at 290nm and 490nm and the emission can be detected at 530nm.
Storage:
Store under dark at 4°C or room temperature.
User Instruction:
DNA SafeStain can be used in the exact same way as EtBr in agarose gel electrophoresis. For example, add 100µl to 1 liter of 1X running buffer (1xTAE or 1x TBE), and use this DNA SafeStain containing buffer to make the agarose gel and run the gel.
Special Notes:
1. For best results, dilute the DNA SafeStain 1:10,000 in your gel running buffer and use this dye-containing running buffer to make your agarose gel and to run the gel.
2. As is the case with EtBr; add more DNA SafeStain, if brighter DNA bands are desired. To view the results, any conventional DNA gel viewing light box or gel documentation system should work well with the DNA SafeStain; although, using a LED light instead of a UV light source is preferred due to the harmful nature of UV light.
This Product is For Research Use Only
*For the proper disposal of this product, follow University or Company Guidelines.
This brand is being discontinued and will only be available while supplies last.
Need great dNTPs at a great price?
Try our PR1MA™ dNTPs!
Bullseye Individual dNTP's
- Ready-to-use molecular grade dNTP solution for use in DNA polymerization, DNA labeling and sequencing processes
- High purity: >98% by HPLC
- Supplied in solution at pH 7.5
- dNTPs are stable at -20°C, avoid multiple freeze/thawing (For long-term usage, aliquoting is recommended)
- Functionally tested with thermostable polymerases
This brand is being discontinued and will only be available while supplies last.
Need great dNTPs at a great price?
Try our PR1MA™ dNTPs
Bullseye dNTP Set
- Mix of dATP, dCTP, dGTP, dTTP
- Each nucleotide is at a concentration of 100 mM
- Ready-to-use molecular grade dNTP solution for use in DNA polymerization, DNA labelling and sequencing processes
- High purity: > 98% by HPLC
- Supplied in solution at pH 7.5
- dNTPs are stable at - 20°C, avoid multiple freeze/thawing (For long-term usage, aliquoting is recommended)
- Functionally tested with thermostable polymerases
dNTP SET
(dATP, dCTP, dGTP, dTTP)
100 mM
Item # | Size |
BE511109 | 20 x 4 x 250 uL |
BE511120 | 4 x 2 mL |
Components Volume dATP (100mM) 250 µL dCTP (100mM) 250 µL dGTP (100mM) 250 µL dTTP (100mM) 250 µL General Description Ready-to-use molecular grade dNTP solution for use in DNA polymerization, DNA labelling and sequencing processes. Features High purity: >98% by HPLC. Supplied in solution at pH 7.5. Storage Conditions dNTPs are stable at 20oC in a constant temperature freezer. Avoid multiple freeze/thawing. For long-term usage, aliquoting is recommended. Quality control Functionally tested with thermostable polymerases. |
This brand is being discontinued and will only be available while supplies last.
- Higher sensitivity
- Use in the same way as EtBr in agarose gel electrophoresis
- Detection of DNA and RNA
- Safest DNA stain by far
- Low cost
- 10,000X
- Excitation wavelengths at 290nm and 490nm
DNA SafeStain Plus is a highly sensitive green fluorescent DNA/RNA staining reagent for detecting nucleic acids in agarose and polyacrylamide gels. This unique stain gives high sensitivity for detection of double-stranded or single-stranded DNA and RNA. Gels can be post-stained or the stain can be added to gels during gel casting or to the gel running buffer. DNA SafeStain Plus has two excitation wavelength peaks at about 290nm and 490nm, and an emission wavelength at 530nm, making it compatible with a standard UV light box, a blue-light transilluminator, or a gel reader equipped with visible light excitation; such as, a 488 nm laser-based gel scanner.
DNA SafeStain Plus is in a 10,000X concentrated format that can be easily diluted 10,000 times for use in precast gel staining, or 5,000 times for use in post gel staining.
This brand is being discontinued and will only be available while supplies last.
Need great (q)PCR Regents at a great price?
Try our PR1MA™ Taq and save!
Bullseye Taq Plus Master Mix
- Pre-optimized ready-to-use PCR reagents
- Less pipetting to avoid contamination
- Quick and easy to set up
- Direct loading for electrophoresis
- Reproducible results
Users only need to add templates and primers, and water if needed. Extra 25 mM magnesium solution is also provided for additional fine adjustments. The Red Mixes contain a non-hazardous inert red dye. The purple mixes contain a non-hazardous inert purple dye. Completed PCR reactions utilizing the D124-R (red dye) or D124-P (purple dye) can be directly loaded into a well of agarose gel or other gels, for electrophoresis. No extra loading buffer is needed.
Storage: 4°C for up to one month, or -20°C for long term storage.
Magnesium Chloride: In general, 1.5 mM MgCl2 is recommended; this may vary with different conditions and primer sets. Some primers/templates may require adjustments for MgCl2 concentration, which can be achieved as shown below:
Final MgCl2 concentration | 1.5 mM | 2.0 mM | 2.5 mM |
Additional 25 mM MgCl2 per 50 uL reaction | 0.0 uL | 1.0 uL | 2.0 uL |
Directions for use: For a 50 uL reaction, use 25 uL of the Taq Master Mix, add template, primers, and water to a final volume of 50 uL. Cycling conditions vary for different templates and primers. To start with, try 30 cycles as follows: denature at 94°C for 30 seconds, anneal around 55°C for 30 seconds, and extend at 72°C for 1 minute / kb. After the PCR cycles, extend at 72°C for another five minutes to complete the PCR. Then store the reaction at 4°C.
1x Composition: 10 mM KCl, 20 mM Tris HCl (pH 9.0), 16 mM (NH4)2SO4, 0.1% Triton X-100, 1.5 mM MgCl2, 200 mM dNTPs, 2.5 units / 25 uL of Taq DNA polymerase, (optional: trace amount of red or purple dye) and enzyme stabilizers.
Looking for other alternatives?
2x Master Mix Kit (1.5 mM MgCl2)
Bullseye Taq DNA Polymerase Mix is a ready-to-use 2x reaction mix. Simply add primers, template, and water to successfully carry out primer extensions and other molecular biology applications.
Bullseye Taq polymerase, the NH4+ buffer system, dNTPs, and magnesium chloride are conveniently present in the Taq DNA Polymerase Mix. (Inert Red Dye is present in BE180303 only)
Bullseye Taq DNA Polymerase Mix offers several advantages. Set up time is significantly reduced. The chance of contaminating component stocks is eliminated. Reduction of reagent handling steps leads to better reproducibility. Standard tests can be set up with the confidence that results will be consistent every time.
Composition of 2x Taq Master Mix
150 mM Tris-HCl pH 8.5, 40 mM (NH4)2S04, 3 mM MgCl2, 0.2%
Tween 20Ò
4 mM dNTPs
2 units/µL AS ONE Taq polymerase
Inert Red Dye & Stabilizer (BE180303 only)
Need a great Reverse Transcriptase product at a great price? Try PR1MA!
Bullseye RT 2X Master Mix
- Up to 9kb cDNA synthesis
- Ensures sample to sample consistency
- Large RNA sample volume capacity
- Ready to use
Size: 100 rxns
RT 2X Master Mix is a proprietary, ready-to-use master mix for first-strand cDNA synthesis in a 2X concentration. This optimized reaction mix contains ribonuclease inhibitor, dNTPs, and a balanced concentration for oligo(dT) and random primers. The ribonuclease inhibitor effectively protects RNA template from degradation. The oligo(dT) anneals selectively to the poly(A) tail of mRNAs and the random primers do not require the presence of poly(A) and they are utilized for the transcription of mRNA 5-end regions. The resultant cDNA can be directly used as template in different PCR experiments.
Kit Components
EasyScript R Tase (200U / uL) | 100 L |
2X Reaction Mix | 1200 uL |
Nuclease-Free H2O | 2 x 1 mL |
Storage
- Store at -20°C in a frost-free freezer.
Item # | Description | Quantity | Rxn |
BERTCDNA-25 | RT 2X Master Mix | 250 uL | 25 rxns |
BERTCDNA-100 | RT 2X Master Mix | 1 mL | 100 rxns |
Application
- cDNA synthesis
- Construction of cDNA libraries
- Generation of probes for hybridization
Protocol
- Thaw RNA templates and all reagents on ice. Mix each solution by vortexing.
- Assemble the following components in a tube on ice, and mix well:
Components | Volume | Final Conc. |
Total RNA, or | Variable | 1 ng - 2 ug/rxn |
mRNA | Variable | 1 pg - 2 ng/rxn |
2X Reaction Mix | 10 uL | 1X |
H2O | Up to 19 uL | - |
- Heat the mixture at 65°C for 5 mins and incubate on ice for at least 1 min.
- Collect all components by a brief centrifugation and add 1 µl of the EasyScript RTase to the tube.
- Mix well and collect all the components by a brief centrifugation.
- Incubate the tube at room temperature for 10 min for annealing.
- Perform cDNA synthesis by incubating the tube for 50 min at 42°C.
- Stop the reaction by heating it at 85°C for 5 min.
- Chill on ice. The newly synthesized first-strand cDNA is ready for immediate downstream applications.