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PCR/qPCR

MIDSCI can supply almost every needed product for PCR/qPCR: tubes, plates, strips, caps, taq, Mastermixes, dNPTs, dye and probe for qPCR, sealing film and much more.  If you are looking for any of these things, we can match almost all needs to all cyclers.  If you don’t find it easily contact us at tech@midsci.com and we will find it for you. 

96 or 384 we have it.  Non-Skirted, semi-skirted or full skirted, we have it.  0.1, 0.2, or 0.5 mL we have it.  A1, A12, H12, A24, P24 cuts, we have them.  Sybr© Green or probe alternative, we have it.  

Pro Tip: Colored plastics do not have any impact on DNA amplification.  But when setting up qPCR it is often recommended to use white plastics.  This will limit or prevent fluorescence refraction.  Light refraction can minimize sensitivity and consistency. 

Pro Tip 2: If you are seeing inconsistencies in PCR/qPCR try PR1MA Pipette Tips.  This will help optimize and reduce variance in the reactions.  Simple test, see the residual liquid left in tips?  This means some of the taq, dNTPs, sample, etc is left in the tip.  PR1MA tips help reduce that significantly and improve your consistency. 

Pro Tip 3: Seeing evaporation in the outer wells of a PCR/qPCR plate?  Sometimes it’s the plate, sometimes it the seal, and sometimes it’s the cycler.  Call us we have solutions.

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Non-Skirted, Low Profile,
Need a great PCR plastic and at a better price? Try PR1MA. 
 Click here to Order Now!
 
  • Plate Characteristics
    • 96 x 0.1mL, Sub-skirted, cuttable
    • Low Profile (0.1mL), Frosted (low signal to noise ratio
    • Extreme Uniformity (EU) Plate Design
    • Uniform wall thickness ensures consistent even heating
    • Thin wall-bottomed tube for rapid heat transfer
  • Ideal paring with EU Caps with "Sealing Ring Band" Design
    • Easy open - easy close with ergonomic caps: B57801, B79701, C79701
    • Caps are significantly easier to apply and remove -- Repetitive Strain Injury (RSI) Friendly.
    • Significant reduction in evaporation due to a wider sealing area.
  • Certified DNA, DNase, RNA, RNase and pyrogen-free

Please give us a call for a sample!

 

Order#

Description

Non-Skirt

 

B70501

Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, Natural, 25/cs

B70509

Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, White, 25/cs

B50501

Pryme PCR Plate, 96-Well, 0.2mL, Non-Skirt, Cuttable, Regular Profile, Frosted, 25/cs

AB70651

Pryme PCR Plate, 96-Well, 0.1mL, Non-Skirt, Low Profile, Frosted, 25/cs

Semi-Skirted

 

B70651

Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, Natural, 25/cs

B70659

Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, White, 25/cs

B50651

Pryme PCR Plate, 96-Well, 0.2mL, Semi-skirted, Cuttable, Regular Profile, Frosted, 25/cs

B17480

Pryme PCR Plate, 96-Well, 0.1mL, Semi-skirted, Low Profile, Frosted, 25/cs

B17489

Pryme PCR Plate, 96-Well, 0.1mL, Semi-skirted, Low Profile, White, 25/cs

Sub-Skirted

 

ABI7501

Pryme PCR Plate, 96-Well, 0.2mL, Sub-skirted, Regular Profile, Frosted, 25/cs

ABI7509

Pryme PCR Plate, 96-Well, 0.2mL, Sub-skirted, Regular Profile, White, 25/cs

AB19800-EU

Pryme PCR Plate, 96-Well, 0.1mL, Sub-skirted, Low Profile, Frosted, 25/cs

AB19809-EU

Pryme PCR Plate, 96-Well, 0.1mL, Sub-skirted, Low Profile, White, 25/cs

Caps

 

B57801

Pryme PCR Ergonomic 8-Strip, Optical Flat (V2) Caps, Natural, 120/cs

B79701

Pryme PCR Ergonomic 8-Strip, Optical Flat (V1) Caps, Natural, 120/cs

C79701

Pryme PCR Ergonomic 8-Strip, Semi-Domed (V1) Caps, Natural, 120/cs
Item#:
AB70651
Your Price:
109.68
Each
This item is discontinued

Pure•Amp™ Color Coded Strip Tubes

Pure•Amp™ 0.2mL PCR tubes and strips are available in seven different configurations including single tubes, eight strips, domed caps, and flat caps. Optically clear caps, and white tubes (for optimal fluorescence reflectivity) are also available for qPCR applications.

Pure•Amp™ 0.2 mL color coded PCR strip tubes are easily identified at a glance, making them the ideal tube to track your samples.

Pure•Amp™ 0.1mL Low Profile PCR tubes and strips are available in three different color/texture configurations, all intended for qPCR applications: Clear, White, and Frosted.

All Pure•Amp™ PCR Tubes and Strips are precision manufactured to maintain tight dimensional tolerances of ultra-thin wall thickness, for improved thermal transfer. Pure•Amp™ PCR Plastics are certified free of RNase, DNase, DNA and PCR inhibitors, and are made of heat resistant medical-grade polypropylene.
  • RNase, DNase, DNA, and PCR inhibitor free
  • 0.2mL or Low Profile 0.1mL options
  • Ultra-thin, uniform wall thickness
  • White and frosted tubes for enhanced qPCR results
Item#:
ASPUREAMPCLRTB
Clear, Flat Top, 25/cs
Please know that the manufacturer has suspended manufacturing this product indefinitely. 
We suggest purchasing PR1MA PCR Plastics.
Contact your rep or email tech@midsci.com for more information.

 
  • Standard thermal cycling and qPCR compatible
  • Ultra-thin and consistent wall thickness for precise thermal transfer
  • Made from 99.9% virgin polypropylene
  • Single notch corner
  • 335ul well capacity (working capacity dependent on closure method)
  • Fits standard thermal cyclers, ABI Real Time PCR 7300, 7500, 7900, qPCR and ABI Sequencing
  • Certified DNAse/RNAse and pyrogen-safe
Item#:
PCR-96-FLT-C
Your Price:
91.69
Each
Convenient, Easy-to-Use Roll format

SealPlate® RollMate™ Pre-scored Film in a Convenient, Easy to Use Roll Format


Pre-scored film on a roll is a more economical and efficient alternative to rapidly seal microplates or racked test tubes; faster than applying single sheeted film. Self-standing, non-slip supports hold the pre-scored film roll securely in place for quick and easy bench-top dispensement. The roll unwind tension can be easily adjusted to user preference. Continuous backing liner can be torn off or fed directly into a waste basket for disposal.
  • Economical
  • Quicker, more efficient sealing
  • Provides a secure seal and is easy to remove
  • Rigid polyester film features good clarity and easy handling
  • Can be used as a temporary seal while awaiting further processing
Applications
  • General laboratory use
  • Short-term storage
  • Incubation
  • ELISA
Temperature Range
  • -40°C to +120°C
Item#:
ASFILMROLLMATE
 
SealPlate® RollMate Features
Specs SealPlate® RollMate
Sealing film selection guide by application
Sealing film selection guide by attributes
Item#:
ASSEALPOLYC

EZcap™FoilStrips


An economical and more user-friendly alternative to PCR strip caps; no sore thumbs and fingers guaranteed!

Application: PCR and light sensitive assays
Temperature Range: -20°C to +120°C

Precision cut to a smaller dimension for sealing one PCR 8-tube strip or a single row of a 96-well PCR or deep-well storage plate whenever rows must be
selectively protected or accessed. FoilStrips remove easily with no splash back as can occur with strip-caps.

  • Certified DNase- & RNase- free
  • Each sheet contains 6 strips on a continuous liner for ease of use
  • Pierceable with a pipet tip or robotic probe for direct sample recovery
  • The conformable nature of foil allows for the visual verification of the seal’s security around each well
  • 2.0mil aluminum foil removes easily without tearing
 
Helpful Tips:

  • A non-locking workstation is recommended for sample prep (e.g. 96-well round-boom plate)
  • Removal of the end-tabs at the perforations is recommended before cycling
  • A final sealing step is highly recommended in the thermal cycler because 8-tube strips flex during handling
  • Do not fold foil around sides of tubes
Item#:
ASEZCAFOIL
 
EZcapFoilStrips Features
Specs EZcapFoilStrips
Sealing film selection guide by application
Sealing film selection guide by attributes

This product is no longer available.

For a different Silicone Sealing Mats, visit here.
 


Silicone Sealing Mat for 96 plates

  • Made of research-grade silicone
  • Scored wells for sample access
  • Perforations re-seal after access
  • Resists coring and tearing
  • Eliminates cross-talk between wells
  • Also works as a compression mat
  • Suitable for standard PCR thermocycling
  • Mats can be reused
  • For use with 96-well PCR plates
  • Printed alpha numeric grid for easy reference
  • Certified to be free of RNase, DNase, DNA, PCR inhibitors, and Pyrogen contamination.

Works as a plate seal (to eliminate cross contamination) and compression mat all-in-one!

Our compression molded 96-round plug, silicone sealing mats for PCR plates minimize sample evaporation in a wide range of temperatures. These mats are chemically resistant and reusable; containing no adhesives or extractables to contaminate samples.  Made of research-grade silicone, they can be autoclaved, and following a 10% bleach wash and ethanol rinse protocol may be reused.

Wells are scored (cut 80% of the way through, but not all of the way) to allow for sample access via pipettor, syringe, or probe.  These scored perforations will seal themselves, after penetration, ensuring an airtight closure to the well.
Item#:
ASSILICONE96MAT
Item#:
ASAHEAT0P8
  • The CleanSpot is designed to fulfill a very important laboratory need...control of PCR contamination.
  • Just 25 - 30 minutes of UV irradiation in the CleanSpot can prevent unwanted PCR contamination.
  • The combination of a special light reflector and two powerful overhead UV bulbs generate Pyrimidine Dimers (T,C) and other photo defects in contaminating target sequences, thus eliminating falsely primed products that result in countless lost time and expense.
  • The CleanSpot is designed to prevent unwanted contaminant DNA in amplification mixtures, thereby saving many lost hours and expensive samples.
  • The UV resistant acrylic enclosure provides a contained, dead air space in which to set up your reactions and permits safe 254nm UV irradiation of solutions and supplies. This powerful UV wavelength renders DNA contaminants biologically inactive in 30 minutes or less.
  • The thick front panel is an effective beta irradiation shield as well as acting as a breath guard. An overhead fluorescent light illuminates the work area.
  • Dimensions: 18"L x 24"W x 28"H
  • 1 year warranty
Item#:
CS01
Your Price:
2984.00
Each
This item is discontinued
Item#:
ASSEAL96CZ3P1
Item#:
ASADSEALP3P1

AlumaSeal® CS™


With a 50 µm layer of acrylic adhesive that withstands cold storage at temperatures to -80 °C.

Unlike other sealing films in the AlumaSeal group, AlumaSeal CS sealing films are not recommended for PCR or thermocycling. A single non-perforated end tab simplifies application. Simply hold the tab and strip the backing from the body of the sealing film as it lays on the plate to avoid curling. Dimensions are 82.6 by 132.6 mm, including the single 9.5 mm end tab. AlumaSeal CS sealing films are certified DNase-, RNase-, and nucleic-acid-free.

  • Specially formulated for cold storage to -80 °C
  • Excellent barrier properties to retard evaporation
  • Easily pierceable with pipet tips or robotic probes for sample recovery
 
*Completing application of AlumaSeal CS sealing films by pressing with the accessory plate roller assures a secure uniform seal around all wells.
Item#:
ASALUMASEL
 
AlumaSeal CS Features
Specs AlumaSeal CS
Sealing film selection guide by application
Sealing film selection guide by attributes
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