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MagBio HighPrep™ DTR Clean-up System

Magnetic beads based reagent for manual and automated Sanger sequencing reaction clean-up for both ABI and MegaBACE sequencing platforms.

Applications
  • Sanger sequencing reaction clean-up / dye terminator removal for:
  • Sanger sequencing on ABI platforms
  • Sanger sequencing on MegaBACE platforms

Benefits
  • Rapid and reliable clean-up of sequencing reactions
  • High DNA recovery yields, long sequence readings
  • Flexible - can be used in any format
  • No centrifugation step, no filtration step
  • Adaptable to common liquid handling workstations
The HighPrep™ DTR Clean-up System is a high performance paramagnetic bead-based reagent for BigDye™ terminator removal from Sanger sequencing reactions for reliable and long readings of DNA sequences. The HighPrep™ DTR Clean-up System consists of a selective binding of DNA to the paramagnetic beads, followed with washing off nucleotides, primers, and non-targeted amplicons, and finally elution of pure DNA. HighPrep™ DTR Clean-up System is an excellent choice for both manual and fully automated purification of sequencing reaction products. The protocol can be adapted to common liquid handling workstation.
Item#:
ASHIPREPDTRCLUS

IBI MIDI I-Blue Plasmid Kit

The I-Blue MINI Plasmid Kit was designed for rapid isolation of plasmid DNA from 1-7 mL of cultured bacterial cells. I-Blue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. Typical yields are 20-35 µg for high-copy number plasmid or 3-10 µg for low-copy number plasmid from 4 mL of cultured bacterial cells. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 15 minutes. The purified plasmid DNA is ready for use in restriction enzyme digestion, ligation, PCR, and sequencing reactions.

Advantages

  • Sample: 1-7 mL of cultured bacterial cells
  • Yield: Up to 50 µg of pure plasmid DNA
  • Format: Plasmid spin column
  • Operation Time: Within 15 minutes
  • Elution Volume: 30-100 µL
  • Kit Storage: Dry at room temperature (15-25°C) for up to 1 year; PD1 and RNase A mixture should be stored at 2-8°C for up to 6 months

Quality Control

The quality of the I-Blue MINI Plasmid Kit is tested on a lot-to-lot basis by isolating plasmid DNA from a 4 mL overnight E. coli (DH5α) culture containing plasmid pBluescript (A600 > 2 U/mL). Following the purification process, a yield of more than 20 µg is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid DNA (1 µg) is used in EcoRI digestion, and analyzed by electrophoresis.
Item#:
ASIBIMIDIPLASMID

MagBio MagCTL Collection Tube

The MagCTL Collection Tube is the first GUANIDINE FREE sample collection and transportation device with FDA 510(K) Clearance and CE/IVDR marking Certification. The proprietary stabilization buffer in the tubes offers room temperature stability of RNA for up to 8 days, as well as direct pathogen lysis and inactivation. The MagCTL Collection Tube has been clinically approved for viral samples but can also be used for bacterial or fungal samples in research studies.

Applications

Viral, Bacterial or Fungal swab sample collection, stabilization and direct lysis for:
  • DNA extraction
  • RNA extraction
  • Pathogen Detection
  • Viral Load Detection
  • RT-PCR
Benefits

SAFETY: 
Guanidine Free Stabilizer
Sample Inactivation

ROOM TEMPERATURE STABILITY:
DNA = 45 Days
RNA = 8 Days

DIRECT LYSIS:
Collected samples are lysed inacitivating the sample and releasing the DNA/RNA making them avaliable for capture using magnetic beads.

REGULATORY CLEARANCE:
FDA 510(K) Approved
CE/IVDR Certified

MULTI-SAMPLE VERSATILITY:
Clinically approved to work with Viral Samples
Adaptable to work with Bacterial OR Fungal Samples

 
The MagCTL Collection Tube is the first Guanidine Free, FDA 510(K) approved and CE/IVDR certified sample collection device that can stabilize DNA & RNA at ambient temperatures. The tube contains a proprietary molecular transport medium which lyses and inactivates collected samples. Direct lysis of the sample effectively stabilizes the nucleic acids (DNA & RNA) making them available for capture without the need of an additional lysis step. Furthermore, direct pathogen lysis eliminates the need for sample processing in a containment making it easier for laboratory staff to handle samples.

The long term ambient stability of DNA & RNA offered by the MagCTL Collection Tubes makes them suitable for clinical settings since it eliminates the need for freezer storage space. Samples can be stored at ambient temperature on the bench top for up to 8 Days for RNA and 45 days for DNA.

Key Features:

Safety - The MagCTL Collection Tube consists of 1.2 mL of the MagBio CTL™ Medium contained in a sterile 5 mL Cryotube. The proprietary medium is GUANIDINE FREE making it safe for use in clinical settings that require cleaning with bleach on a regular basis. In light of the widespread use of guanidine based collection devices due to the COVID-19 Pandemic, the FDA published this article warning sample processing facilities to avoid using guanidine based collection devices.

Stability - The MagCTL Collection Tube validation study showed that RNA is stabilized for up to 8 days at room temperature. However, further research studies on this product showed stability of both RNA and DNA. Stability of RNA was 14 days and DNA stabilization was 45 days at room temperature. This allows for samples to be collected and transported without the need for cold storage at any point.

Direct Lysis – The direct lysis feature is the most unique feature of the MagCTL Collection Tube. Collected samples are lysed upon collection and the nucleic acids are released making them available for capture. This allows end users to skip the lysis step in extraction workflows. The released DNA / RNA can be captured using the MAG-S2 collection beads manufactured by MagBio. The direct lysis also adds to safety because the virus is inactivated and during sample processing there is no need for the use of a containment.

Multi Sample Versatility – The MagCTL Collection Tube is a versatile device that can be used to collect and analyze Viral, Bacterial OR Fungal samples.

FDA & CE/IVDR Clearance – The MagCTL Collection Tube is the first non-hazardous, FDA 510(K) approved sample collection device that offers direct lysis and can stabilize nucleic acids at ambient temperatures. This makes the tube a suitable candidate for clinical laboratories performing pathogen detection or sequencing. In addition to FDA Clearance, the MagCTL Collection Tube is also CE/IVDR Certified.

Downstream Benefits – The MagCTL Collection Tube allows end users to significantly cut down time needed for downstream processes. Samples collected in the MagXtract Collection Tube skip the lysis process and are directly captured in magnetic beads (MAG-S2) followed by washes and elution in nuclease free water. The extraction efficiency of MagCTL Collection Tube is comparable to that of Thermofisher MVP II Kit where lysis buffer is used. This proves that the MagCTL Tubes can be critical in reducing both time & cost associated with nucleic acid extraction.

Item#:
ASMAGCTLCOLTUB
Sample Kit, 4 preps

Isolation of micro RNA (miRNA) from Tissue or Cultured Cells

The miRNA Isolation Kits are designed for purification of micro RNA (miRNA) and other small cellular RNAs from tissue samples or cultured cells. Purification of miRNA allows research into biological significant pathways for gene regulation. The standard protocol for isolating total RNA and mRNA are not optimized for isolation of small RNA molecules and result in the loss of substantial amounts of miRNA and other small RNA. 

In addition, removal of the predominantly larger RNAs is required for accurate analysis of miRNA expression by qPCR or microarray analysis. This kit is specifically designed for purification of small RNA with minimal contamination from large RNA molecules or genomic DNA. The method employs a spin column with a silica-based fiber matrix that binds RNA in the presence of a chaotropic salt. The method is based on the selective binding of RNA molecules of various sizes to the silica-based fiber matrix when different ethanol concentrations are present in the solvent.

Sample Types:
100mg tissue or 1 x 10 cultured cells
Format:
Spin column

Operation Time:
30 minutes
Item#:
IB47370
Your Price:
30.42
Each

IBI Small DNA Fragment


The Gel/PCR DNA Fragments Extraction Kits are designed to recover or concentrate DNA fragments (50bp-10kb) from agarose gel, PCR reaction, or any other enzymatic reaction. This method uses a chaotropic salt and guanidine thiocyanate to dissolve the agarose gel and denature the enzymes. The DNA fragments in the chaotropic salt are bound to the glass-fiber matrix of the spin column. After washing off the contaminants, the purified DNA fragments are eluted by low salt elution buffer or water. Salts, enzymes, and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation.
Item#:
ASIBISMALLDNAB

IBI Small DNA Fragment


The Gel/PCR DNA Fragments Extraction Kits are designed to recover or concentrate DNA fragments (50bp-10kb) from agarose gel, PCR reaction, or any other enzymatic reaction. This method uses a chaotropic salt and guanidine thiocyanate to dissolve the agarose gel and denature the enzymes. The DNA fragments in the chaotropic salt are bound to the glass-fiber matrix of the spin column. After washing off the contaminants, the purified DNA fragments are eluted by low salt elution buffer or water. Salts, enzymes, and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation.
Item#:
ASIBISMALLDNAA
Kit, 100 preps

Isolation of micro RNA (miRNA) from Tissue or Cultured Cells

The miRNA Isolation Kits are designed for purification of micro RNA (miRNA) and other small cellular RNAs from tissue samples or cultured cells. Purification of miRNA allows research into biological significant pathways for gene regulation. The standard protocol for isolating total RNA and mRNA are not optimized for isolation of small RNA molecules and result in the loss of substantial amounts of miRNA and other small RNA. 

In addition, removal of the predominantly larger RNAs is required for accurate analysis of miRNA expression by qPCR or microarray analysis. This kit is specifically designed for purification of small RNA with minimal contamination from large RNA molecules or genomic DNA. The method employs a spin column with a silica-based fiber matrix that binds RNA in the presence of a chaotropic salt. The method is based on the selective binding of RNA molecules of various sizes to the silica-based fiber matrix when different ethanol concentrations are present in the solvent.

Sample Types:
100mg tissue or 1 x 10 cultured cells
Format:
Spin column

Operation Time:
30 minutes
Item#:
IB47371
Your Price:
579.66
Each

MagBio HighPrep™ Blood & Tissue DNA Kit


 
Blood and tissue DNA kit, a magnetic bead-based kit for high-quality genomic DNA extraction from 50-250 µL of fresh or frozen whole blood, buffy coat, lysate of tissues, mouse tails, cultured cells, saliva, buccal swabs.


Applications

Blood and tissue gDNA extraction for:

  •     PCR, Real-time PCR
  •     Southern Blotting
  •     Cloning, Genotyping
  •     Sequencing

Benefits

  •     High-quality DNA from blood and tissue samples
  •     No organic extraction or alcohol precipitation
  •     Complete removal of contaminants and inhibitors
  •     Adaptable to various automated workstations



Blood and Tissue DNA Kit has been designed to isolate high quality genomic DNA from 50-250 µL of fresh or frozen whole blood, buffy coat, lysate of tissues, mouse tails, cultured cells, saliva, buccal swabs.

HighPrep™ Blood & Tissue DNA Kit utilizes paramagnetic beads to isolate genomic DNA (gDNA) from various sample types including tissue, whole blood, saliva and cells; resulting in high quantity and quality DNA usable in various genomic downstream applications. HighPrep™ Blood & Tissue DNA Kit could be used for few samples as well as in high throughput setting on automated workflow. 
Item#:
ASHIHPRPBLOTISDK

MagBio HighPrep™ Viral RNA Kit


Magnetic beads based kit for rapid isolation of viral nucleic acids from whole blood, serum, plasma, saliva and other body fluids. 


Applications

Viral RNA isolation for:

  •     RT-qPCR; RT-PCR, PCR
  •     One-Step RT-qPCR
  •     Virus detection, genotyping
  •     Viral load monitoring,

Benefits

  •     OPTIMIZED FOR ISOLATION from VIRAL samples.
  •     Rapid and reliable purification of nucleic acids
  •     Adaptable to various automated liquid handling workstations
  •     No toxic organic solvents
 

The HighPrep™ Viral RNA Kit is designed for rapid and reliable isolation of viral nucleic acids from serum, plasma, saliva and other body fluids as well as nasopharyngeal swabs soaked in virus transport media or other buffers. This kit is highly efficient in vIral nucleic acid isolation and the extracted RNA is suitable for direct use in most downstream applications such as one-step RT-qPCR, RT-PCR, PCR, nucleic acid amplification, cloning, sequencing, and enzymatic reactions. The kit can be used in low throughput manual workflows and is also adaptable to majority of the liquid handling workstations in the market.
Item#:
ASHIHPRPVIRRNAK

REPLACEMENT PD COLUMNS AND COLLECTION TUBES -100 PACK

  • For use with leftover reagents from IBI Plasmid Kits or competitive products
  • Sample Size: 1 -4 mL bacterial culture
  • Elution Volume: 50 to 100 µL
  • Binding Capacity: Up to 30 µg

The Replacement Mini Plasmid Columns and Collection Tubes are for use with leftover reagents from IBI Mini Plasmid Kits including the following item numbers: IB47101, IB47102, IB47170, IB47171, and IB47172.

The columns and collection tubes can also be used with comparable, competitive Mini Plasmid Kit reagents that utilize a bind, wash, and elute method. However, you must observe the sample size, binding capacity, and elution volume for the replacement column stated in the specifications.

SPECIFICATIONS

Sample Size: 1 - 4 mL bacterial culture
Format: Spin column or vacuum manifold
Expected yield: 20 -30 µg for high copy and 3 -10 µg for low copy.
Elution volume: 50 µL to 100 µL
Operation time: 15 minutes
Purified plasmid DNA is ready for use in ligation, PCR, restriction enzyme digestion, and sequencing reactions.

Item#:
ASIB47107
 
Protocal

REPLACEMENT DF COLUMNS AND COLLECTION TUBES - 100 PACK

  • For use with leftover reagents from IBI PCR/Gel Extraction Kits or competitive products
  • Sample Size: 300 mg agarose gel/100 µL PCR product
  • Elution Volume: 20 to 50 µL
  • Binding Capacity: Up to 10 µg

The Replacement DF Columns and Collection Tubes are for use with leftover reagents from IBI PCR/Gel Extraction Kits including the following item numbers: IB47020 and IB47030.

The replacement spin columns and collection tubes can also be used with comparable, competitive PCR/Gel Extraction Kit reagents that utilize a bind, wash, elute method. However, you must observe the sample size, binding capacity, and elution volume for the replacement column stated in the specifications.

SPECIFICATIONS

Sample Size: 300 mg of agarose gel or 100 µL of PCR product
Format: Spin column or vacuum manifold
Expected yield: 90% for Gel extraction and 95% for PCR Clean-up
Elution volume: 20 µL to 50 µL
Operation time: 20 minutes
Purified DNA is ready for use in Fluorescent or Radioactive sequencing, ligation, PCR, restriction enzyme digestion, and DNA labeling.

Item#:
ASIB47082
 
Protocal

 MagBio HighPrep™ Plant DNA Plus Kit


Magnetic beads based kit designed to extract high-quality genomic DNA from plants (leaves, roots, seeds).


Applications

Plant genomic DNA isolation for:

  •     PCR, Real-time PCR
  •     Cloning, genotyping
  •     Sequencing
  •     Plant breeding

Benefits

  •     Purified PCR grade DNA with no inhibiting substances (polysaccharides and phenols)
  •     Includes beads to homogenize sample
  •     Eliminate need of grinding
  •     Adaptable to automation

The HighPrep™ Plant DNA Plus Kit is specially designed for purifying DNA from a wide range of plant and fungi species. The kit uses the special lysis condition with HighPrep™ magnetic particles technology to isolate the high-quality genomic DNA. Under DNA binding conditions only the genomic DNA will bind to the magnetic particles while most of the contaminating cellular proteinaceous components are removed. The purifi ed DNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, Southern blotting, SNP analysis and NGS etc. The HighPrep™ Plant DNA Plus Kit (Magnetic Bead System) can be easily adapted to automated magnetic bead separation instruments and work stations.
Item#:
ASHIHPRPPLTDNA
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