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IBI 96-Well Vacuum Manifold

  • Optimized for use with all of IBI 96-well kits
  • The thin upper portion of the manifold is designed to reduce cross contamination
  • Allows for the most effective extraction and purification of plasmid DNA, genomic DNA, viral DNA & RNA, total RNA and PCR products
  • Materials: Manifold - made of anodized aluminum, Gasket - Ethylene propylene, O-Ring - Silicone
  • Dimensions: 17x12x8cm
  • Maximum vacuum: Approx. 71cm Hg (28 in Hg) (-13.7psi)
Item#:
ASIBIVACU96WELL

MagBio MagQuant™ Plus DNA V2


Magnetic bead based kit for normalization of DNA concentration, and quantitation of DNA for NGS and other applications


Applications

DNA quantitation and normalization of DNA concentration for:

  •     Next Gen Sequencing library construction
  •     Amplicon normalization
  •     Genomic DNA normalization
  •     PCR, qPCR

Benefits

  •     Rapid and reliable quantitation and normalization of DNA
  •     No centrifugation step, no filtration step
  •     Eliminates the need for standard curve
  •     Allowing the processing of pool DNA samples from various sources
  •     Equilizing input genomic DNA concentration for DNA libray construction to help produce consistent and reliable NGS data without tedious initial input DNA quantitation.

The MagQuant™ Plus DNA V2 Kit utilizes paramagnetic bead-based technology for genomic DNA quantitation and concentration normalization.  The DNA normalization occurs during the purification process.  Standard methods of DNA quantitation require the use of a standard curve derived from DNA samples of known concentrations; processing DNA samples from various sources would require extensive work and reagent use. MagQuant™ Plus DNA V2‘s proprietary technology allows to obtain a given DNA quantitation and required concentration of DNA samples regardless of the source by adding a specific number of beads to the DNA sample.
Item#:
ASMAGQUANTPDNA2
4 x 96 Well Format

High Throughput PCR Clean-Up


The 96-well PCR Clean-Up/DNA Extraction Kits provide a high-throughput, rapid and economical method to purify DNA fragments. Chaotropic salt is used to denature enzymes and in this condition, DNA fragments are bound by the glass fiber matrix in each well of the plate. Once the contaminants have been removed, the purified DNA is eluted by a low salt elution buffer or water. Salts, enzymes, and unincorporated nucleotides are effectively removed from reaction mixtures without toxic phenol extraction or alcohol precipitation. This entire protocol can be completed in 30-40 minutes, and the eluted DNA is ready to use in restricted digestion, ligation, PCR, and sequencing reactions.


Sample Size:

Up to 50µl of PCR product
Up to 50mg of agarose slice

Format:

96-Well Plates

Operation:

Centrifuge/Vacuum manifold

Binding Capacity:

10ug per well

DNA Size:

50bp-10kb

Recovery:

80-90% for Gel Extraction
90-95% for PCR Clean-Up

Operation Time:

30 min for PCR Clean-Up
40 min for Gel Extraction
Item#:
IB47040
Your Price:
608.35
Each

MagBio HighPrep™ PCR Clean-up System


 Magnetic beads based reagent for manual and automated post-PCR and post-enzymatic reaction clean-up, for NGS library clean-up and reliable amplicon size selection.
 
Applications

Post-PCR and post-enzymatic reaction clean-up used for/during:
  •     NGS library preparation
  •     Amplicon size selection for NGS
  •     Microarrays
  •     PCR
  •     Restriction enzyme digestions, adapter ligations
  •     Cloning
Benefits

  •     Rapid and reliable post-PCR and post enzymatic reaction clean-up
  •     Efficient wash from contaminants
  •     High recovery of amplicons >100bp
  •     Uniform fragments size distribution
  •     Adaptable to high throughput liquid handling workstations

The HighPrep™ PCR Clean-up System is a paramagnetic beads-based post PCR clean-up reagent designed for an efficient purification of DNA fragments and tight size-specific selection of amplicons. The purification consists of removal of salts, primers, primer-dimers, dNTPs, as DNA fragments are selectively bound to the magnetic bead particles. Highly purified DNA is eluted with low salt elution buffer or water which can be used directly for downstream applications, such as library construction, next generation sequencing (NGS), Sanger  sequencing, cloning, restriction digestions, adapoter ligation, microarrays, and so on. This protocol can be used for manual procedure as well as as a guideline for adapting the kit to automatic liquid handling instruments. The HighPrep™ PCR Clean-up Reagent is perfectly suited for amplicon size selection, and NGS library preparation for sequencing as well as for post PCR clean-up of shorter amplicons or restriction enzyme digestion products.
Item#:
ASHIHPRPPCRCUS
10 x 96 Well Format

High Throughput PCR Clean-Up


The 96-well PCR Clean-Up/DNA Extraction Kits provide a high-throughput, rapid and economical method to purify DNA fragments. Chaotropic salt is used to denature enzymes and in this condition, DNA fragments are bound by the glass fiber matrix in each well of the plate. Once the contaminants have been removed, the purified DNA is eluted by a low salt elution buffer or water. Salts, enzymes, and unincorporated nucleotides are effectively removed from reaction mixtures without toxic phenol extraction or alcohol precipitation. This entire protocol can be completed in 30-40 minutes, and the eluted DNA is ready to use in restricted digestion, ligation, PCR, and sequencing reactions.


Sample Size:

Up to 50µl of PCR product
Up to 50mg of agarose slice

Format:

96-Well Plates

Operation:

Centrifuge/Vacuum manifold

Binding Capacity:

10ug per well

DNA Size:

50bp-10kb

Recovery:

80-90% for Gel Extraction
90-95% for PCR Clean-Up

Operation Time:

30 min for PCR Clean-Up
40 min for Gel Extraction
Item#:
IB47050
Your Price:
1425.45
Each
Extraction Kit (50-200bp),

fficient Gel Extraction and PCR Clean-up

The Gel/PCR DNA Fragments Extraction Kits are designed to recover or concentrate DNA fragments (50bp-10kb) from agarose gel, PCR reaction, or any other enzymatic reaction. This method uses a chaotropic salt and guanidine thiocyanate to dissolve the agarose gel and denature the enzymes. The DNA fragments in the chaotropic salt are bound to the glass-fiber matrix of the spin column. After washing off the contaminants, the purified DNA fragments are eluted by low salt elution buffer or water. Salts, enzymes, and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation.

Sample:
up to 300mg agarose gel slice
up to 100µl PCR product or other enzymatic reaction
Format:
Spin columns
Operation:
Centrifuge/Vacuum Manifold
Binding Capacity:
10µg DNA
Expectant Yield:
80-90% for gel extraction
90-95% for PCR clean-up
Operational Time: 15min. for PCR clean-up/20min. for gel extraction
Applications:
PCR, Fluorescent or Radioactive Sequencing, Restriction Digests, DNA Labeling, Ligations
Item#:
IB47062
Your Price:
465.57
Each
Extraction Sample Kit,

fficient Gel Extraction and PCR Clean-up

The Gel/PCR DNA Fragments Extraction Kits are designed to recover or concentrate DNA fragments (50bp-10kb) from agarose gel, PCR reaction, or any other enzymatic reaction. This method uses a chaotropic salt and guanidine thiocyanate to dissolve the agarose gel and denature the enzymes. The DNA fragments in the chaotropic salt are bound to the glass-fiber matrix of the spin column. After washing off the contaminants, the purified DNA fragments are eluted by low salt elution buffer or water. Salts, enzymes, and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation.

Sample:
up to 300mg agarose gel slice
up to 100µl PCR product or other enzymatic reaction
Format:
Spin columns
Operation:
Centrifuge/Vacuum Manifold
Binding Capacity:
10µg DNA
Expectant Yield:
80-90% for gel extraction
90-95% for PCR clean-up
Operational Time: 15min. for PCR clean-up/20min. for gel extraction
Applications:
PCR, Fluorescent or Radioactive Sequencing, Restriction Digests, DNA Labeling, Ligations
Item#:
IB47060
Your Price:
30.56
Each

    IBI DNA/RNA/Protein Extraction Kits

  • Sample Size: up to 5×106 cultured animal cells/up to 25mg animal tissue /up to 500µl whole blood/up to 200µl biological fluids (serum, plasma)
  • Expectant Yield: up to 9µg of genomic DNA/20µg of total RNA/120µg of protein from 1×106 of HeLa cells
  • Operation Time: DNA/RNA purification within 25 minutes, protein precipitation within 50 minutes
  • Elution Volume: 50  200µl (genomic DNA)/25  50µl (total RNA)
  • Format: genomic DNA spin column and total RNA spin column

The IBI All Prep DNA RNA Protein Mini Kit provides an efficient method for purifying genomic DNA, total RNA, and total protein simultaneously from cultured cells, animal tissues, whole blood, and biological fluids. Chaotropic salt is used to lyse cells and inactivate DNases and RNases, allowing DNA to bind to the genomic DNA spin column. The flow-through can then be transferred to the RNA Spin column for RNA binding. Contaminants are effectively removed using wash buffers followed by pure genomic DNA elution in a low salt buffer and pure total RNA elution in RNase-free water.

DNA/RNA purification can be completed in 25 minutes without phenol/chloroform extraction or alcohol precipitation, and protein purification can be completed in 50 minutes. The purified DNA, with approximately 20  30Kb, is suitable for use in PCR or other enzymatic reactions and the purified RNA (including miRNA) is ready for use in RT-PCR, Real-Time PCR, northern blotting, primer extension, mRNA selection, and cDNA synthesis. The purified proteins can be directly analyzed on a SDS-PAGE and subsequent western blot.

The quality of the All Prep DNA RNA Protein Mini Kit is tested on a lot-to-lot basis by isolating genomic DNA and total RNA from 1x106 cultured animal cells. The purified DNA and total RNA is quantified with a spectrophotometer and analyzed by electrophoresis on a 1% agarose gel.  The purified protein is quantified by Bradford assay analyzed on SDS-PAGE.

Specifications

Sample: Cultured cells, animal tissue, whole blood & biological fluids
Format: Genomic DNA spin column and Total RNA spin column.
Yield: up to 9ug of Genomic DNA / 20ug of Total RNA / 120ug of Protein from 1 x 106 HeLa cells.
Operation Time: Within 25 minutes
Elution volume: 50  200ul for Genomic DNA / 25  50ul for Total RNA.
Simultaneous extraction of Genomic DNA and Total RNA from cultured cells, animal tissues, whole blood and biological fluids.

Item#:
ASDNARNAKIT40

MagBio HighPrep™ Viral DNA/RNA Kit


Magnetic beads based kit for rapid isolation of total viral nucleic acids from whole blood, serum, plasma, saliva and other body fluids. 
OPTIMIZED FOR ISOLATION OF SARS-CoV-2 RNA


Applications

Viral RNA and DNA isolation for:

  •     RT-qPCR; RT-PCR, PCR
  •     One-Step RT-qPCR
  •     Virus detection, genotyping
  •     Viral load monitoring,

Benefits

  •     OPTIMIZED FOR ISOLATION OF SARS-CoV-2 RNA
  •     Rapid and reliable purification of viral nucleic acids
  •     Adaptable to various automated liquid handling workstations
  •     No toxic organic solvents

The HighPrep™ Viral DNA/RNA kit is designed for rapid and reliable isolation of total viral nucleic acids from whole blood, serum, plasma, saliva and other body fluids as well as nasopharyngeal swabs soaked in virus transport media or other buffers. This kit is highly efficient in vIral nucleic acid isolation and the extracted RNA (and DNA) is suitable for direct use in most downstream applications such as one-step RT-qPCR, RT-PCR, PCR, nucleic acid amplification, cloning, sequencing, and enzymatic reactions. The kit can be used in low throughput manual workflows and is also adaptable to majority of the liquid handling workstations in the market.
Item#:
ASHIHPRPVIRDNRNK
  • Sample: Cultured cells
  • Yield: High yield, High quality DNA (A260/A280 = 1.8-2.0)
  • Format: Scalable DNA precipitation method
  • Kit Storage: Dry at room temperature (15-25°C) for up to 2 years, RNase A should be stored at 4°C for extended periods

The gPURE DNA Isolation Kit offers a simple and gentle reagent DNA precipitation method for isolating high molecular weight genomic, mitochondrial or viral DNA suitable for archiving or sensitive downstream applications. This highly versatile solution based system can be scaled proportionately in order to satisfy larger sample volumes providing a convenient sample-storage procedure with minimal hands on time. Initially cells are lysed in the presence of detergents and a proprietary DNA stabilization solution followed by RNase A treatment. Once proteins and other contaminants are removed DNA is precipitated then rehydrated. The high quality extracted DNA is ready for use in a variety of downstream applications.

Quality Control
gPURE DNA Isolation Kits are tested on a lot-to-lot basis by isolating DNA from (3-5 x 106) cultured cells. The isolated DNA (5-15 μg with an A260/A280 ratio of 1.8â2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.

Components and Storage
Cell Lysis Buffer, Protein Removal Buffer, DNA Hydration Buffer should be stored dry at room
temperature (15-25°C) for up to 1 year. RNase A should be stored at 4°C for extended periods.


gPURE DNA Isolation Kit

IB47430

IB47431

IB47432

Number of Cells Processed Per Kit

2 x 10

6 x 10

6 x 10

Cell Lysis Buffer

3 mL

100 mL

1000 mL

Protein Removal Buffer

1 mL

40 mL

400 mL

DNA Hydration Buffer
(10 mM Tris-HCl, 1mM EDTA, pH 8.0)

1 mL

50 mL

500 mL

RNase A (10 mg/mL)

25 µL

550 µL

5 mL

Cell Number

0.5-1 x 10 

3-5 x 10 

3.5 x 10

Tube Size

1.5 mL

1.5 mL

15 mL

Cell Lysis Buffer

150 µL

600 µL

6 mL

RNase A (10 mg/mL)

1 µL

3 µL

30 µL

Protein Removal Buffer

50 µL

200 µL

2 mL

Isopropanol

150 µL

600 µL

6 mL

70% Ethanol

150 µL

600 µL

6 mL

DNA Hydration Buffer

50 µL

100 µL

200 µL
Item#:
ASDNARNAKIT22
  • Sample: up to 25 mg of tissue, up to 25 mg of paraffin-embedded tissue
  • Yield: 5-30 µg
  • Format: Spin column
  • Operation time: Within 20 minutes

Introduction
The Total RNA Mini Kit (Tissue) was designed specifically for purifying total RNA from a variety of animal and paraffin-embedded tissue. Tissue samples can be efficiently homogenized in a microcentrifuge tube using the provided micropestle. Detergents and chaotropic salt are used to lyse cells and inactivate RNase and optional DNase treatments can be followed to remove unwanted DNA residue. RNA in the chaotropic salt is bound by the glass fiber matrix of the spin column (1). Once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-Free Water, and is ready for use in RT-PCR, Northern Blotting, Primer Extension and cDNA Library Construction. Phenol extraction or alcohol precipitation is not required.

Quality Control 
The quality of the Total RNA Mini Kit (Tissue) is tested on a lot-to-lot basis by isolating total RNA from a 25 mg animal tissue sample. The purified RNA is quantified with a spectrophotometer and checked by electrophoresis.

Kit

IB47300

IB47301

IB47302

IB47303

RB Buffer

2 mL

30 mL

60 mL

130 mL

W1 Buffer

2 mL

30 mL

50 mL

130 mL

Wash Buffer*
(Add EtOH)

1 mL
(4 mL)

12.5 mL
(50 mL)

25 mL
(100 mL)

50 mL x 2
(200 mL x 2)

RNase-free H2O

1 mL

6 mL

15 mL

30 mL

RB Column

4 pcs

50 pcs

100 pcs

300 pcs

Filter Column

4 pcs

50 pcs

100 pcs

300 pcs

2 mL Collection Tubes

8 pcs

100 pcs

200 pcs

600 pcs

Micropestle

4 pcs

50 pcs

100 pcs

300 pcs

*Add absolute ethanol (see the bottle label for volume) to the Wash Buffer prior to initial use.

Caution 
RB Buffer contains chaotropic salt which is a harmful irritant. During operation, always wear a lab coat, disposable gloves, protective goggles, and (anti-fog) procedure mask.

Item#:
ASDNARNAKIT21

Introduction
IBI Isolate is a phenol, chloroform and guanidine isothiocyanate based scalable solution for extracting high-quality total RNA as well as simultaneous extraction of RNA, DNA and protein from a wide variety of samples such as blood, buffy coat, plasma, serum, cultured cells and tissue. The extracted RNA can be used directly in a variety of downstream applications such as cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays and Northern Blotting.

Quality Control
IBI Isolate is tested on a lot-to-lot basis. RNA from a 1 ml human blood sample is extracted using IBI Isolate. 10 µL from a 50 µL eluate of RNA is analyzed by electrophoresis on a 0.8% agarose gel.

Advantages
 Extract total RNA or simultaneous RNA, DNA and protein within 1 hour
 Sample: up to 300 µL (blood, buffy coat, serum, plasma), up to 5 x 106 (cultured cells), 50-100 mg (tissue)
 Scalable
 Format: phenol, chloroform and guanidine isothiocyanate

Applications
cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays and Northern Blotting

Caution
IBI Isolate contains phenol and guanidine isothiocyanate. During operation, always work in a fume hood, always wear a lab coat, disposable gloves, protective goggles and (anti-fog) procedure mask. Disposable/non-disposable glassware, plasticware and automatic pipettes should be sterile (RNase-free) and used only for RNA procedures.

Additional Requirements
RNA Extraction: chloroform, isopropanol, 70% ethanol, RNase-free Water, 1.5 mL microcentrifuge tubes (RNase-free) DNA Extraction: chloroform, absolute ethanol, 70% ethanol, sodium citrate/ethanol solution (0.1 M sodium citrate in 10% ethanol, pH 8.5), 8 mM NaOH solution or TE Buffer pH 8.5, 1.5 mL microcentrifuge tubes

Components and Storage
IBI Isolate is shipped at room temperature and can be stored dry at 2°C to 25°C for up to 9 months.

IB47600

IBI Isolate Total DNA & RNA Extraction Reagent, Sample 4 mL

IB47601

IBI Isolate Total DNA & RNA Extraction Reagent, 100 mL

IB47602

IBI Isolate Total DNA & RNA Extraction Reagent, 200 mL
Item#:
ASDNARNAKIT24
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