Nitrocellulose Membranes

Combining the advantages of high protein binding capacity with low background and high membrane stability, which ensures easy handling.

Ideal for western blotting, DNA blotting as well as dot or slot blots. They have been optimized for all protein blotting systems, such as electro transfer, semi-dry or simple capillary blotting

Specifications:

Protran nitrocellulose membranes are the most frequently specified transfer media in the world for a wide range of applications. Protran membranes are manufactured using 100% pure nitrocellulose to ensure the highest binding capacity possible. Other membranes referred to as "nitrocellulose" may actually contain large amounts of cellulose acetate, which will lower the protein binding capacity. In addition to high binding capacity, Protran nitrocellulose membranes inherently have very low background. The superior surface properties of the membrane guarantees superior signal-to-noise ratios, without the need for stringent washing conditions. Unlike PVDF membranes, Protran nitrocellulose requires no methanol pre-wetting step. This makes it the membrane of choice for proteins that prefer aqueous environments. Prior to transfer, the membrane is simply wet in water and then in the transfer buffer, with no other necessary pre-treatment steps. Protran membranes exhibit the best handling strangth of all pure nitrocellulose membranes.
Features:

• High binding capacity (80-150g/cm2) of proteins and nucleic acids.
• 100% pure nitrocellulose, with no cellulose acetate added, ensures the highest binding capacity possible.
• Compatible with virtually all standard detection methods.
• Low background - NC surface properties guarantee superior signal-to-noise ratios.
• Triton-free matrix with low extractable levels will support the cell growth essential for colony blotting, plaque lifts, and tissue culture applications.
• Available in many sizes and formats to fit different transfer devices and gel chambers.