Western Blot Boxes

• The perfect box for every blot
• Reduce antibody usage
• The hinged Lid minimizes evaporation
• For halves, wholes, strips, or minis

No matter what size your Western blot is, there's a box that fits it perfectly. With boxes that are correctly matched to common blot sizes, you can fit more blots on shakers/rockers while keeping reagent and antibody usage to an absolute minimum. Additionally, all corners are formed at 90° angles and the bottom surface is uniformly flat, further reducing consumption of costly reagents and antibodies (for example, popular mini gels require only about 4 mL).

At a price that makes them truly disposable, all sizes are engineered from crystal clear polystyrene. The exception is our opaque black boxes (except WBB7BK and WBB15BK), which protect light-sensitive samples. All sizes are also equipped with an easy-to-open hinged lid that reduces evaporation.

Specifications
 

Western Lightning Plus (NEL103):
·  Twice the sensitivity of standard products 

·  Direct protocol transfer 

·  ~ 1 picogram sensitivity

·  > 2 hr signal duration for re-exposing film

·  Working solution stability 8 hr at room temperature 

·  PVDF or Nitrocellulose membranes 

·  Non-toxic (no msds required)

Western Lightning Pro (NEL120)
·  Mid-range sensitivity, with broad dynamic range

·  Detection of less than 1 picogram amount of protein

·  Long, stable signal > 12 hr for re-exposing film 

·  Stable at room temperature >1 year 

·  Working solution stable for > 24 hr at room temperature

·  Non-toxic (no msds required) 

Western Blot 2.0 with PURITY™

The PURITY™ detection system revolutionizes Western Blotting in three steps.
 

Blocking buffer and secondary antibody are already components of the PURITY™ Reagent. And the washing buffer is also included. The only thing to add is your specific primary antibody.

Benefits:

• 1 simple protocol which makes almost all optimization unnecessary.
• Increased sensitivity and reproducibility of blots.
• Dramatically reduces the number of steps and time required for Western blots.
• Drastically decreases background phenomena.
• Highly reliable.

• Used for agarose electrophoresis of DNA, RNA or nucleic acids
• Contains 3 tracking dyes and 15% Ficoll in a special Tris dye

• Light blue - around 4000bp in 1% agarose
• Indigo - around 600bp in 1% agarose
• Magenta - around 150bp in 1% agarose

• DNase/RNase/Protease free

IB01015
5X RNA Gel Loading Kit

• Reagents for denaturing and loading RNA samples onto a formaldehyde gel, using MOPS as a buffer
• RNA sample is dissolved in 10µl of DEPC water and mixed with 35µl of denaturing solution. Heat the sample to 65°C for 5 min. Once the solution has cooled, add 5µl of loading dye. The sample is now ready to load into the gel.
• DNase/RNase/Protease free

IB01190
2X Protein Loading Dye

• Tracks the migration progression of your sample during polyacrylamide electrophoresis
• Loading dye migrates independently of the samples, making it easier to estimate the migration of proteins
• DNase/RNase/Protease free

IB72120
Xylene Cyanol FF

• Used as a tracking dye at 5kb to monitor the progress of electrophoresis separation
• CAS Number: 2650-17-1
• DNase/RNase/Protease free

IB74040
Bromophenol Blue

• Tracking dye in electrophoretic separations
• Migrates around 0.5kb
• CAS Number: 115-39-9
• Purity: >98.0%

RunBlue SDS Running Buffer (NXB50500) a TEO-Tricine buffer system which provides a separation similar to the MOPS buffer used with BIS-TRIS precast gels. It provides enhanced separation of higher molecular weight proteins. The buffer can be for reduced and non-reduced samples.

RunBlue TGS Blot Buffer(NXB82600) has been specially formulated to provide optimal blot transfer of proteins from RunBlue Precast Gels. Supplied as a concentrate should be diluted by a factor of 10 for use with the RunBlue Dual Run and Blot system or a factor of 20 for use with other blotting methods.