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HS Taq Polymerase, 2X Mix
with HS Buffer I
Cat.No. |
Size Reactions |
HS Taq, 2X Mix (Buffer I) |
Final MgCl2 Conc. |
BE230301 |
100 |
2X HS Buffer I Mix |
1.5mM |
BE230303 |
500 |
2X HS Buffer I Mix |
1.5mM |
BE230304 |
1,000 |
2X HS Buffer I Mix |
1.5mM |
BE230306 |
2,500 |
2X HS Buffer I Mix |
1.5mM |
Store at -20°C. For in-vitro laboratory use only
General Description
Bullseye HS Taq Polymerase, 2X Mix is a ready-to-use 2.0X master mix. Simply add primers, template, and water to successfully carry out primer extensions and other molecular biology applications.
Bullseye HS Taq Polymerase Mix, the NH4+ buffer system, dNTPs and magnesium chloride are present in HS Taq Pol Mix with HS Buffer I. Each reaction requires 25 µL of the 2.0X reaction mix. Simply add primers, template and water to a total reaction volume of 50 µL.
Bullseye" HS Taq Polymerase Mix is a modified form of Bullseye Taq DNA Polymerase, which is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during setup and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended. The result is higher specificity and greater yields when compared to standard DNA polymerases.
Bullseye" HS Taq Polymerase Mix offers several advantages. Set up time is significantly reduced. The chance of contaminating component stocks is eliminated. Reduction of reagent handling steps leads to better reproducibility. Standard tests can be set up with the confidence that results will be consistent every time.
Composition of HS Taq Pol, 2x Mix
Tris-HCl pH 8.5, (NH4)2S04, 3.0mM MgCl2, 0.2% Tween 20Ã, 0.4 mM dNTPs, 0.2 units/µL HS Hot Start DNA Polymerase
Stabilizer
Suggested Protocol using HS Taq Pol, 2x Mix
This protocol serves as a guideline only. Optimal reaction conditions may vary and must be individually determined.
-
Set up reaction mixtures in an area separate from that used for DNA preparation or product analysis.
-
The table below shows the reaction set up for a final volume of 50 mL.
-
Important: Mix the solutions completely before use to avoid localized concentrations of salts.
1. Set up each reaction as follows:
Component |
Vol./Reaction |
Final Conc. |
HS Hot Start Master Mix with HS Bufffer I |
25 µL |
1X |
Primer A |
Variable |
0.11.0 µM |
Primer B |
Variable |
0.11.0 µM |
Distilled Water |
Variable |
- - - - |
Template DNA |
Variable |
Variable |
TOTAL volume |
50 µL |
- - - - |
2. Mix gently by pipetting the solution up and down a few times.
3. Program the thermal cycler according to the manufacturer's instructions.
4. Each program must start with an initial heat activation step at 95°C for 15 minutes.
For maximum yield and specificity, temperatures and cycling times should be optimized for each new template target or primer pair.
A typical thermal cycling program is shown below:
95°C for 15 min. Activate HS Hot Start Polymerase
30-40 cycles:
95°C 30 sec Denature template
45-65°C 30 sec Anneal primer
72°C 1-5 min Elongation
72°C for 5 min Elongation
5. Place the tubes in the thermal cycler and start the reaction.
Gel Cutting Tips
Our Gel Cutting Tips are specially designed to provide an easy, efficient solution for removing bands from agarose gel. Made from high-quality, non-pyrogenic polypropylene, these tips are ideal for applications where contamination-free performance is essential.
- Effortless Band Removal: Perfect for safely extracting bands from agarose gels without damage.
- One-Handed Operation: Designed for ease of use, allowing you to operate with just one hand for faster, more efficient workflows.
- Push Button Gel Release: The convenient push-button design ensures smooth gel band release with minimal effort.
- Ejector Button for Tip Release: Easily eject the tip with a quick press to maintain a clean, contamination-free environment.
- Avoid Cross-Contamination: Features a secure and precise fit to minimize the risk of cross-contamination.
- Universal Compatibility: Compatible with standard 1,000 µL pipettors for seamless integration into your lab setup.
- Non-Pyrogenic & RNase-/DNase-Free: Ensures that your experiments are free from unwanted contamination, preserving the integrity of your samples.
- Non-Sterile: Intended for use in controlled environments where sterilization is not required.
Specifications
| Item # | TGL-1165 |
| Packaging Format | 250 Tips/Pk, 10 Packs/Cs |
| Sterile | No |
| Autoclavable | Yes |
| Color | Clear |
Soft and non-permeable, the 1.4 mil aluminum sealing foil offers optimum sealing for all PCR plates. Using a medical-grade acrylic adhesive, our foil seals are easily pierced with single or multi-channel pipettors and robotic probes. These soft foil seals, resist rolling back when removing the backing paper and seal tightly to both PCR and Deep Well Blocks.
Ideally suited for PCR and Cold Storage down to -80 °C. Exceptional adhesive properties and foil material durability yield virtually no sample evaporation or drying.
Certified free of RNase, DNase, Pyrogen, and nucleic-acid contamination.
Length |
133.35 mm |
Length (w/ end tabs removed) |
120.65 mm |
Width |
80.01 mm |
Overall Thickness |
1.4 mil |
Adhesive Thickness |
1.1 mil |
Material |
Aluminum |
Adhesive |
Medical Grade Acrylic |
Max. Temperature |
120 °C |
Min. Temperature |
-80 °C |
Primary Usages |
Storage or Sample Cover |
This brand is being discontinued and will only be available while supplies last.
Need great (q)PCR Regents at a great price?
Try our PR1MA™ Taq and save!
- Excellent all-purpose amplification enzyme
- Thermostable recombinant DNA polymerase from Thermus aquaticus
- Exhibits very high activity in primer extension
- Has both a 5' to 3' DNA polymerase and a 5' to 3' exonuclease activity
- Does not have 3' to 5' exonuclease activity-no proofreading ability
- Leaves an A-overhang, which makes the enzyme ideal for TA cloning
- Includes MgCl² in buffer
This brand is being discontinued, please contact us for other options.
Looking for other alternatives?
Visit our PR1MA Polymerase page for more options!
Store at -20°C. For in-vitro laboratory use only
General Description
Bullseye Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa.
Bullseye Taq DNA Polymerase has both a 5'®3' DNA polymerase and a 5'®3' exonuclease activity. The enzyme lacks a 3'®5' exonuclease activity (no proofreading ability). Taq DNA Polymerase leaves an A¢ overhang, which makes the enzyme ideal for TA cloning.
The 10X Reaction Buffer provided does not contain Mg+2. 25 mM MgCl2 is supplied separately.
10X Mg++ Free Standard Buffer
100 mM Tris-HCl pH 8.3, 500 mM KCl.
Unit Definition
One unit is defined as the amount that incorporates 10 nmoles of dNTPs into acid-precipitable form in 30 minutes at 72°C under standard assay conditions.
Storage and Dilution Buffer
Enzyme is supplied in 20 mM Tris-HCl pH 8.3, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% Tween 20, 0.5%NP40, 50% glycerol.
Quality Control
Each lot of Taq DNA Polymerase is tested for contaminating activities, with no trace of endonuclease activity, nicking activity, exonuclease activity or priming activity.
Suggested Protocol using Taq DNA Polymerase
This protocol serves as a guideline only. Optimal reaction conditions may vary and must be individually determined.
n In some applications, MgCl2 is needed for the best results. For this reason, 25 mM MgCl2 is included with the kit.
1. Thaw 10X Mg2+ Free Standard Buffer, dNTP mix, primer solutions. It is important to mix the solutions completely before use to avoid localized concentrations of salts.
2. Prepare a master mix according to Table 1. The master mix typically contains all the components needed for extension except the template DNA.
Table 1. Reaction components (master mix & template DNA)
Component |
Vol./reaction |
Final Conc. |
10X Mg2+ Free Buffer |
5 µl |
1X |
MgCl2, 25 mM |
1- 9 µl |
0.5 4.5 mM |
dNTP mix (12.5 mM of each) |
0.8 µl |
0.2 mM of each dNTP |
Primer A |
Variable |
0.11.0 µM |
Primer B |
Variable |
0.11.0 µM |
Taq DNA Polymerase |
Variable |
1-5 units |
Template DNA |
Variable |
Variable |
Distilled Water |
Variable |
- - - - |
TOTAL volume |
50 µl |
- - - - |
Table 2. MgCl2 concentration in a 50 mL reaction
Final MgCl2 conc. in reaction (mM) |
0.5 |
1.0 |
1.5 |
2.0 |
2.5 |
3.0 |
3.5 |
4.0 |
4.5 |
Volume of 25 mM MgCl2 per rxn (µl): |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
Table 2 provides the volume of 25 mM MgCl2 to add to the master mix if a certain MgCl2 concentration is required.
3. Mix the master mix thoroughly and dispense appropriate volumes into reaction tubes. Mix gently, e.g., by pipetting the master mix up and down a few times.
4. Add template DNA to the individual tubes containing the master mix.
5. Program the thermal cycler according to the manufacturers instructions. For maximum yield and specificity, temperatures and cycling times should be optimized for each new template target or primer pair.
6. Place the tubes in the thermal cycler and start the reaction.
Looking for other alternatives?
2x Master Mix Kit (1.5 mM MgCl2)
Bullseye Taq DNA Polymerase Mix is a ready-to-use 2x reaction mix. Simply add primers, template, and water to successfully carry out primer extensions and other molecular biology applications.
Bullseye Taq polymerase, the NH4+ buffer system, dNTPs, and magnesium chloride are conveniently present in the Taq DNA Polymerase Mix. (Inert Red Dye is present in BE180303 only)
Bullseye Taq DNA Polymerase Mix offers several advantages. Set up time is significantly reduced. The chance of contaminating component stocks is eliminated. Reduction of reagent handling steps leads to better reproducibility. Standard tests can be set up with the confidence that results will be consistent every time.
Composition of 2x Taq Master Mix
150 mM Tris-HCl pH 8.5, 40 mM (NH4)2S04, 3 mM MgCl2, 0.2%
Tween 20Ò
4 mM dNTPs
2 units/µL AS ONE Taq polymerase
Inert Red Dye & Stabilizer (BE180303 only)
This brand is being discontinued, please contact us for other options.
Looking for other alternatives?
Visit our PR1MA Polymerase page for more options!
R-Taq DNA Polymerase 5 units/µL
| Cat. No. | Units | 10X Ammonium Buffer (MgCl2 15mM) | MgCl2 25 mM |
| BE200303 | 500 | 1.5 mL | 1.5 mL |
| BE200304 | 1,000 | 2x 1.5 mL | 2x 1.5 mL |
| BE200306 | 2,500 | 4x 1.5 mL | 4x 1.5 mL |
General Description
Bullseye R-Taq DNA Polymerase is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. R-Taq contains a red dye which provides easy and quick identification of reactions to which enzyme was added and allows confirmation of complete mixing. The inert dye has no effect on downstream processes. R-Taq is added directly to the reaction mix and is used in the same manner as standard Taq DNA Polymerase.
Bullseye R-Taq DNA Polymerase has both a 5'®3' DNA polymerase and a 5'®3' exonuclease activity. The enzyme lacks a 3'®5' exonuclease activity. R-Taq DNA Polymerase leaves an A-overhang, which makes the enzyme ideal for TA cloning.
- High performance thermostable DNA polymerase
- Red dye identifies tubes which contain enzyme and confirms complete mixing of reagents
- Leaves an A-overhang
Unit Definition
One unit is defined as the amount that incorporates 10 nmoles of dNTPs into acid-precipitable form in 30 minutes at 72°C under standard assay conditions.
Storage Buffer
Enzyme is supplied in 20 mM Tris-HCl pH 8.3, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, inert dye, 0.5 % Tweenà 20, 0.5% NP40, 50% glycerol.
Component | Vol./reaction | Final Conc. |
10X Ammonium Buffer | 5 µL | 1X |
dNTP mix (12.5 mM each) | 0.8 µL | 0.2 mM each dNTP |
Primer A | Variable | 0.1-0.5 µM |
Primer B | Variable | 0.1-0.5 µM |
R-Taq DNA Pol | 1 mL | 5 units/reaction |
Distilled Water | Variable | - - - - |
Template DNA | Variable | 0.1-0.5 µg/reaction |
TOTAL volume | 50 µL | - - - - |
Table 2. MgCl2 concentration
3. Mix the master mix thoroughly and dispense appropriate volumes into reaction tubes. Mix gently (e.g., by pipetting) the master mix up and down a few times.
4. Add template DNA (0.1-0.5 mg/reaction) to the individual tubes containing the master mix.
5. Program the thermal cycler according to the manufacturer's instructions.
For maximum yield and specificity, temperatures and cycling times should be optimized for each new template target or primer pair.
6. Place the tubes in the thermal cycler and start the reaction.
7. After primer extension, load 5-10 mL of a 50 mL reaction directly on an agarose gel for analysis.
Tween 20 is a registered trademark of ICI Americas, Inc.
Final MgCl2 conc. in reaction (mM) | 1.5 | 2.0 | 2.5 | 3.0 | 3.5 | 4.0 | 4.5 |
Additional volume of 25 mM MgCl2 per reaction (µL): | 0 | 1 | 2 | 3 | 4 | 5 | 6 |
10X Ammonium Reaction Buffer
Tris-HCl pH 8.5, (NH4)2S04, 15 mM MgCl2, 1% Tween 20
Quality Control
Endonuclease, exonuclease and priming activities are not detected after 3 hours incubation of 1 mg of pUC19 plasmid DNA and 0.5 µg EcoR I digested lambda phage DNA at 72°C in the presence of 40 units of R-Taq DNA Polymerase.
Suggested Protocol using R-Taq Polymerase
This protocol serves as a guideline. Optimal reaction conditions such as incubation times, temperatures, and amount of template DNA may vary and must be individually determined.
1. Thaw 10X Ammonium Buffer, dNTP mix, and primer solutions. It is important to mix the solutions completely before use to avoid localized concentrations of salts.
2. Prepare a master mix according to Table 1. The master mix typically contains all the components needed for extension except the template DNA.
The optimal MgCl2 concentration should be determined empirically but, in most cases a concentration of 1.5 mM, as provided in the 1X Ammonium Buffer, produces satisfactory results. Table 2 provides the volume of 25mM MgCl2 to add to the master mix if a higher MgCl2 concentration is required.
Table 1. Reaction components (master mix & template DNA)
PCR SILICONE SEALING MAT - 96
- Made of research-grade silicone
- Scored wells for sample access
- Perforations re-seal after access
- Resists coring and tearing
- Eliminates cross-talk between wells
- Also works as a compression mat
- Suitable for standard PCR thermocycling
- Mats can be reused
- For use with 96-well PCR plates
- Printed alpha numeric grid for easy reference
- Certified to be free of RNase, DNase, DNA, PCR inhibitors, and Pyrogen contamination.
- 10 Mats/Bag, 5 Bags/Case
Works as a plate seal (to eliminate cross contamination) and compression mat all-in-one!
Our compression molded 96-round plug, silicone sealing mats for PCR plates minimize sample evaporation in a wide range of temperatures. These mats are chemically resistant and reusable; containing no adhesives or extractables to contaminate samples. Made of research-grade silicone, they can be autoclaved, and following a 10% bleach wash and ethanol rinse protocol may be reused.
Wells are scored (cut 80% of the way through, but not all of the way) to allow for sample access via pipettor, syringe, or probe. These scored perforations will seal themselves, after penetration, ensuring an airtight closure to the well.
These Rotocycler strips are perfectly designed to perform on Rotor-Gene instruments. Tube strips are packaged separately from cap strips. The frosted extensions on caps not only make them more efficient and secure during handling but also offer a convenient area for labeling. For individual use, tube and cap strips can easily be separated and used as individual units.
MagBio HighPrep™ Viral/Pathogen DNA/RNA Kit
Magnetic beads based kit for rapid isolation of viral, bacterial and fungal nucleic acids from whole blood, serum, plasma, saliva and other body fluids.
- RT-qPCR; RT-PCR, PCR
- One-Step RT-qPCR
- Virus detection, genotyping
- Viral load monitoring,
- OPTIMIZED FOR ISOLATION from FUNGAL, BACTERIAL and VIRAL samples.
- Rapid and reliable purification of nucleic acids
- Adaptable to various automated liquid handling workstations
- No toxic organic solvents
The HighPrep™ Viral/Pathogen DNA/RNA kit is designed for rapid and reliable isolation of viral, bacterial and fungal nucleic acids from whole blood, serum, plasma, saliva and other body fluids as well as nasopharyngeal swabs soaked in virus transport media or other buffers. This kit is highly efficient in vIral nucleic acid isolation and the extracted RNA (and DNA) is suitable for direct use in most downstream applications such as one-step RT-qPCR, RT-PCR, PCR, nucleic acid amplification, cloning, sequencing, and enzymatic reactions. The kit can be used in low throughput manual workflows and is also adaptable to majority of the liquid handling workstations in the market.
, Beads
The I-Blue MINI Plasmid Kit was designed for rapid isolation of plasmid DNA from 1-7 mL of cultured bacterial cells. I-Blue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. Typical yields are 20-35 µg for high-copy number plasmid or 3-10 µg for low-copy number plasmid from 4 mL of cultured bacterial cells. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 15 minutes. The purified plasmid DNA is ready for use in restriction enzyme digestion, ligation, PCR, and sequencing reactions.
Advantages
- Sample: 1-7 mL of cultured bacterial cells
- Yield: Up to 50 µg of pure plasmid DNA
- Format: Plasmid spin column
- Operation Time: Within 15 minutes
- Elution Volume: 30-100 µL
- Kit Storage: Dry at room temperature (15-25°C) for up to 1 year; PD1 and RNase A mixture should be stored at 2-8°C for up to 6 months
Quality Control
The quality of the I-Blue MINI Plasmid Kit is tested on a lot-to-lot basis by isolating plasmid DNA from a 4 mL overnight E. coli (DH5α) culture containing plasmid pBluescript (A600 > 2 U/mL). Following the purification process, a yield of more than 20 µg is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid DNA (1 µg) is used in EcoRI digestion, and analyzed by electrophoresis.
The I-Blue MINI Plasmid Kit was designed for rapid isolation of plasmid DNA from 1-7 mL of cultured bacterial cells. I-Blue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. Typical yields are 20-35 µg for high-copy number plasmid or 3-10 µg for low-copy number plasmid from 4 mL of cultured bacterial cells. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 15 minutes. The purified plasmid DNA is ready for use in restriction enzyme digestion, ligation, PCR, and sequencing reactions.
Advantages
- Sample: 1-7 mL of cultured bacterial cells
- Yield: Up to 50 µg of pure plasmid DNA
- Format: Plasmid spin column
- Operation Time: Within 15 minutes
- Elution Volume: 30-100 µL
- Kit Storage: Dry at room temperature (15-25°C) for up to 1 year; PD1 and RNase A mixture should be stored at 2-8°C for up to 6 months
Quality Control
The quality of the I-Blue MINI Plasmid Kit is tested on a lot-to-lot basis by isolating plasmid DNA from a 4 mL overnight E. coli (DH5α) culture containing plasmid pBluescript (A600 > 2 U/mL). Following the purification process, a yield of more than 20 µg is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid DNA (1 µg) is used in EcoRI digestion, and analyzed by electrophoresis.
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