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PCR SILICONE SEALING MAT - 96
- Made of research-grade silicone
- Scored wells for sample access
- Perforations re-seal after access
- Resists coring and tearing
- Eliminates cross-talk between wells
- Also works as a compression mat
- Suitable for standard PCR thermocycling
- Mats can be reused
- For use with 96-well PCR plates
- Printed alpha numeric grid for easy reference
- Certified to be free of RNase, DNase, DNA, PCR inhibitors, and Pyrogen contamination.
Works as a plate seal (to eliminate cross contamination) and compression mat all-in-one!
Our compression molded 96-round plug, silicone sealing mats for PCR plates minimize sample evaporation in a wide range of temperatures. These mats are chemically resistant and reusable; containing no adhesives or extractables to contaminate samples. Made of research-grade silicone, they can be autoclaved, and following a 10% bleach wash and ethanol rinse protocol may be reused.
Wells are scored (cut 80% of the way through, but not all of the way) to allow for sample access via pipettor, syringe, or probe. These scored perforations will seal themselves, after penetration, ensuring an airtight closure to the well.
These Rotocycler strips are perfectly designed to perform on Rotor-Gene instruments. Tube strips are packaged separately from cap strips. The frosted extensions on caps not only make them more efficient and secure during handling but also offer a convenient area for labeling. For individual use, tube and cap strips can easily be separated and used as individual units.
MagBio HighPrep™ Viral/Pathogen DNA/RNA Kit
Magnetic beads based kit for rapid isolation of viral, bacterial and fungal nucleic acids from whole blood, serum, plasma, saliva and other body fluids.
- RT-qPCR; RT-PCR, PCR
- One-Step RT-qPCR
- Virus detection, genotyping
- Viral load monitoring,
- OPTIMIZED FOR ISOLATION from FUNGAL, BACTERIAL and VIRAL samples.
- Rapid and reliable purification of nucleic acids
- Adaptable to various automated liquid handling workstations
- No toxic organic solvents
The HighPrep™ Viral/Pathogen DNA/RNA kit is designed for rapid and reliable isolation of viral, bacterial and fungal nucleic acids from whole blood, serum, plasma, saliva and other body fluids as well as nasopharyngeal swabs soaked in virus transport media or other buffers. This kit is highly efficient in vIral nucleic acid isolation and the extracted RNA (and DNA) is suitable for direct use in most downstream applications such as one-step RT-qPCR, RT-PCR, PCR, nucleic acid amplification, cloning, sequencing, and enzymatic reactions. The kit can be used in low throughput manual workflows and is also adaptable to majority of the liquid handling workstations in the market.
, Beads
The I-Blue MINI Plasmid Kit was designed for rapid isolation of plasmid DNA from 1-7 mL of cultured bacterial cells. I-Blue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. Typical yields are 20-35 µg for high-copy number plasmid or 3-10 µg for low-copy number plasmid from 4 mL of cultured bacterial cells. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 15 minutes. The purified plasmid DNA is ready for use in restriction enzyme digestion, ligation, PCR, and sequencing reactions.
Advantages
- Sample: 1-7 mL of cultured bacterial cells
- Yield: Up to 50 µg of pure plasmid DNA
- Format: Plasmid spin column
- Operation Time: Within 15 minutes
- Elution Volume: 30-100 µL
- Kit Storage: Dry at room temperature (15-25°C) for up to 1 year; PD1 and RNase A mixture should be stored at 2-8°C for up to 6 months
Quality Control
The quality of the I-Blue MINI Plasmid Kit is tested on a lot-to-lot basis by isolating plasmid DNA from a 4 mL overnight E. coli (DH5α) culture containing plasmid pBluescript (A600 > 2 U/mL). Following the purification process, a yield of more than 20 µg is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid DNA (1 µg) is used in EcoRI digestion, and analyzed by electrophoresis.
The I-Blue MINI Plasmid Kit was designed for rapid isolation of plasmid DNA from 1-7 mL of cultured bacterial cells. I-Blue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. Typical yields are 20-35 µg for high-copy number plasmid or 3-10 µg for low-copy number plasmid from 4 mL of cultured bacterial cells. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 15 minutes. The purified plasmid DNA is ready for use in restriction enzyme digestion, ligation, PCR, and sequencing reactions.
Advantages
- Sample: 1-7 mL of cultured bacterial cells
- Yield: Up to 50 µg of pure plasmid DNA
- Format: Plasmid spin column
- Operation Time: Within 15 minutes
- Elution Volume: 30-100 µL
- Kit Storage: Dry at room temperature (15-25°C) for up to 1 year; PD1 and RNase A mixture should be stored at 2-8°C for up to 6 months
Quality Control
The quality of the I-Blue MINI Plasmid Kit is tested on a lot-to-lot basis by isolating plasmid DNA from a 4 mL overnight E. coli (DH5α) culture containing plasmid pBluescript (A600 > 2 U/mL). Following the purification process, a yield of more than 20 µg is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid DNA (1 µg) is used in EcoRI digestion, and analyzed by electrophoresis.
PCR DNA Clean-up Kit
The 96-well PCR Clean-Up/DNA Extraction Kits provide a high-throughput, rapid and economical method to purify DNA fragments. Chaotropic salt is used to denature enzymes and in this condition, DNA fragments are bound by the glass fiber matrix in each well of the plate. Once the contaminants have been removed, the purified DNA is eluted by a low salt elution buffer or water. Salts, enzymes, and unincorporated nucleotides are effectively removed from reaction mixtures without toxic phenol extraction or alcohol precipitation. This entire protocol can be completed in 30-40 minutes, and the eluted DNA is ready to use in restricted digestion, ligation, PCR, and sequencing reactions.
PCR DNA Clean-up Kit
The 96-well PCR Clean-Up/DNA Extraction Kits provide a high-throughput, rapid and economical method to purify DNA fragments. Chaotropic salt is used to denature enzymes and in this condition, DNA fragments are bound by the glass fiber matrix in each well of the plate. Once the contaminants have been removed, the purified DNA is eluted by a low salt elution buffer or water. Salts, enzymes, and unincorporated nucleotides are effectively removed from reaction mixtures without toxic phenol extraction or alcohol precipitation. This entire protocol can be completed in 30-40 minutes, and the eluted DNA is ready to use in restricted digestion, ligation, PCR, and sequencing reactions.
MagBio HighPrep™ Insect DNA Kit
Magnetic beads based kit designed to extract high-quality genomic DNA from insects, and arthropods (from one leg or wing to the whole body).
- PCR, Real-time PCR
- Cloning, genotyping
- Sequencing
- Insecticide resistance studies
- Efficient isolation of PCR-quality DNA from insects, and arthropods
- No organic solvents
- Allows field epidemiologic research
- Magnetic beads based chemistry adaptable to automation
The HighPrep™ Insect DNA Kit is a high quality paramagnetic bead-based genomic DNA extraction kit used to isolate gDNA from a variety of insect-derived materials (wings, legs, whole body), and from different insect species, such as mosquitoes, flies, ants, and others. The HighPrep™ Insect DNA Kit is based on a fast and simple procedure which can either be performed manually or automated - up to 96 samples of tissues including legs and wings, can be processed in less than an hour.
, Beads
MagBio HighPrep™ RNA Elite Clean-up System
Magnetic beads based reagent for manual and automated clean-up or concentration of RNA (including miRNA, siRNA, aRNA) and single-stranded cDNA after enzymatic reactions or prior sequencing.
- cDNA synthesis
- RT-PCR
- Sequencing
- In vitro transcription
- RNA probe synthesis
- miRNA and siRNA preparation
- Rapid and reliable clean-up of ss cDNA and RNA, including miRNA, siRNA, and aRNA
- Efficient purification of both large and small RNAs, and cDNA
- Complete removal of salts, unincorporated primers, and nucleotides
- No centrifugation step, no filtration step
- Adaptable to your current liquid handling workstation
, Beads
MagBio cfKapture™ Kit
Magnetic bead-based DNA extraction kit designed to isolate circulating cell free DNA (cfDNA) from human plasma and serum.
- Liquid biopsy research
- NIPT genomic research
- Cancer genomic research
- Transplant monitoring
- Efficient Isolation of nucleic acids, with high input and low elution volumes
- No carrier RNA
- Low sample volume processing
- Efficient recovery of small sized fragmented DNA
- No organic extraction or ethanol precipitation
, Beads
- Optimized for use with all of IBI 96-well kits
- The thin upper portion of the manifold is designed to reduce cross contamination
- Allows for the most effective extraction and purification of plasmid DNA, genomic DNA, viral DNA & RNA, total RNA and PCR products
- Materials: Manifold - made of anodized aluminum, Gasket - Ethylene propylene, O-Ring - Silicone
- Dimensions: 17x12x8cm
- Maximum vacuum: Approx. 71cm Hg (28 in Hg) (-13.7psi)
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