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PCR DNA Clean-up Kit


The 96-well PCR Clean-Up/DNA Extraction Kits provide a high-throughput, rapid and economical method to purify DNA fragments. Chaotropic salt is used to denature enzymes and in this condition, DNA fragments are bound by the glass fiber matrix in each well of the plate. Once the contaminants have been removed, the purified DNA is eluted by a low salt elution buffer or water. Salts, enzymes, and unincorporated nucleotides are effectively removed from reaction mixtures without toxic phenol extraction or alcohol precipitation. This entire protocol can be completed in 30-40 minutes, and the eluted DNA is ready to use in restricted digestion, ligation, PCR, and sequencing reactions.
Item#:
ASPCRCLEANKITB

PCR DNA Clean-up Kit


The 96-well PCR Clean-Up/DNA Extraction Kits provide a high-throughput, rapid and economical method to purify DNA fragments. Chaotropic salt is used to denature enzymes and in this condition, DNA fragments are bound by the glass fiber matrix in each well of the plate. Once the contaminants have been removed, the purified DNA is eluted by a low salt elution buffer or water. Salts, enzymes, and unincorporated nucleotides are effectively removed from reaction mixtures without toxic phenol extraction or alcohol precipitation. This entire protocol can be completed in 30-40 minutes, and the eluted DNA is ready to use in restricted digestion, ligation, PCR, and sequencing reactions.
Item#:
ASPCRCLEANKITA

MagBio HighPrep™ Insect DNA Kit


Magnetic beads based kit designed to extract high-quality genomic DNA from insects, and arthropods (from one leg or wing to the whole body).


Applications

Genomic DNA isolation from insects for:

  •     PCR, Real-time PCR
  •     Cloning, genotyping
  •     Sequencing
  •     Insecticide resistance studies

Benefits

  •     Efficient isolation of PCR-quality DNA from insects, and arthropods
  •     No organic solvents
  •     Allows field epidemiologic research
  •     Magnetic beads based chemistry adaptable to automation

The HighPrep™ Insect DNA Kit is a high quality paramagnetic bead-based genomic DNA extraction kit used to isolate gDNA from a variety of insect-derived materials (wings, legs, whole body), and from different insect species, such as mosquitoes, flies, ants, and others. The HighPrep™ Insect DNA Kit is based on a fast and simple procedure which can either be performed manually or automated - up to 96 samples of tissues including legs and wings, can be processed in less than an hour.
Item#:
ASHIHPRPINSECTDK

MagBio HighPrep™ RNA Elite Clean-up System


Magnetic beads based reagent for manual and automated clean-up or concentration of RNA (including miRNA, siRNA, aRNA) and single-stranded cDNA after enzymatic reactions or prior sequencing.


Applications

RNA and ss cDNA clean-up or concentration for or after:

  •     cDNA synthesis
  •     RT-PCR
  •     Sequencing
  •     In vitro transcription
  •     RNA probe synthesis
  •     miRNA and siRNA preparation

Benefits

  •     Rapid and reliable clean-up of ss cDNA and RNA, including miRNA, siRNA, and aRNA
  •     Efficient purification of both large and small RNAs, and cDNA
  •     Complete removal of salts, unincorporated primers, and nucleotides
  •     No centrifugation step, no filtration step
  •     Adaptable to your current liquid handling workstation

The HighPrep™ RNA Elite Clean-up System utilizes MagBio’s solid-phase paramagnetic bead-based technology for high-throughput purification of RNA or cDNA for in vitro applications such as transcription, antisense RNA (aRNA) amplification as well as for RNA and cDNA probe synthesis. This protocol enables recovery even of micro RNA (miRNA), small RNA and total RNA from enzymatic reactions, concentrating of miRNA and total RNA from a diluted sample. The HighPrep™ RNA Elite Clean-up System can be used for manual RNA clean-up as well as on automated liquid handling workstations.
Item#:
ASHIHPRPRNAECUS

MagBio cfKapture™ Kit


Magnetic bead-based DNA extraction kit designed to isolate circulating cell free DNA (cfDNA) from human plasma and serum.


Applications

cfDNA (ccfDNA) extraction for:
  •     Liquid biopsy research
  •     NIPT genomic research
  •     Cancer genomic research
  •     Transplant monitoring

Benefits

  •     Efficient Isolation of nucleic acids, with high input and low elution volumes
  •     No carrier RNA
  •     Low sample volume processing
  •     Efficient recovery of small sized fragmented DNA
  •     No organic extraction or ethanol precipitation

 
The cfKapture™ kit is a magnetic bead-based DNA extraction kit designed to isolate circulatingcell freeDNA (cfDNA) from human plasma and serum. The kit has a quick, simple, and automation friendly protocol that allows users to quickly extract high quality cfDNA that is ready for use in downstream applications such as qPCR, NGS, and bisulfite sequencing.

 
Item#:
ASCFKAPTKIT
  • Optimized for use with all of IBI 96-well kits
  • The thin upper portion of the manifold is designed to reduce cross contamination
  • Allows for the most effective extraction and purification of plasmid DNA, genomic DNA, viral DNA & RNA, total RNA and PCR products
  • Materials: Manifold - made of anodized aluminum, Gasket - Ethylene propylene, O-Ring - Silicone
  • Dimensions: 17x12x8cm
  • Maximum vacuum: Approx. 71cm Hg (28 in Hg) (-13.7psi)
Item#:
IB47500
Your Price:
2411.10
Each

MagBio HighPrep™ Plant RNA Plus Kit


Magnetic beads based kit designed to extract high-quality RNA from plants (leaves, roots, seeds).


Applications

Plant genomic RNA isolation for:
  • PCR, Real-time PCR
  • Cloning, genotyping
  • Sequencing
  • Plant breeding

Benefits
  • Purified PCR grade RNA with no inhibiting substances (polysaccharides and phenols)
  • Includes beads to homogenize sample
  • Eliminate need of grinding
  • Adaptable to automation

The HighPrep™ Plant RNA Plus Kit is specially designed for purifying RNA from a wide range of plant and fungi species. The kit uses the special lysis condition with HighPrep™ magnetic particles technology to isolate the high-quality RNA. Our kit's unique binding conditions ensure that only RNA will bind to the magnetic particles while most of the contaminating cellular proteinaceous components are removed. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, Southern blotting, SNP analysis and NGS etc. The HighPrep™ Plant RNA Plus Kit (Magnetic Bead System) can be easily adapted to automated magnetic bead separation instruments and work stations.
Item#:
ASMAGBIOHIPLANTR

MagBio CTL™ Medium (Collect - Transport - Lyse)


Applications
  • Sample collection, lysis, inactivation and stabilzation for - Viral, Fungal & Bacterial Samples (Swabs)
  • DNA & RNA sample preparation for pathogen detection
  • Flexibility in sample processing - Room Temperature Stability
  • DNA = 45 Days | RNA = 8 Days
  • COVID-19 Detection

Benefits
  • Significantly shortens sample preparation time in pathogen testing & detection workflows
  • Cost savings - Extraction & extraction accessories not required
  • Preserves and maintains the integrity of nucleic acids at room temperature:
  • DNA = 45 Days | RNA = 8 Days
  • Compatible with various extraction or purification workflows
  • Samples will be lysed during transport - No need for extraction / lysis.
  • Allows samples to be batched which can in turn improve the processing efficiency 
Time is always of the essence when it comes to pathogen detection and with MagBio CTL™, you can increase the efficiency and stability of your pathogen samples. Our Medium can be purchased in liters for bulk applications and can be integrated into a variety of workflows. Save time and money with MagBio CTL™.
Item#:
ASMAGBIOCTLMED

IBI MIDI I-Blue Plasmid Sample 

The I-Blue MINI Plasmid Kit was designed for rapid isolation of plasmid DNA from 1-7 mL of cultured bacterial cells. I-Blue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. Typical yields are 20-35 µg for high-copy number plasmid or 3-10 µg for low-copy number plasmid from 4 mL of cultured bacterial cells. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 15 minutes. The purified plasmid DNA is ready for use in restriction enzyme digestion, ligation, PCR, and sequencing reactions.

Advantages

  • Sample: 1-7 mL of cultured bacterial cells
  • Yield: Up to 50 µg of pure plasmid DNA
  • Format: Plasmid spin column
  • Operation Time: Within 15 minutes
  • Elution Volume: 30-100 µL
  • Kit Storage: Dry at room temperature (15-25°C) for up to 1 year; PD1 and RNase A mixture should be stored at 2-8°C for up to 6 months

Quality Control
The quality of the I-Blue MINI Plasmid Kit is tested on a lot-to-lot basis by isolating plasmid DNA from a 4 mL overnight E. coli (DH5α) culture containing plasmid pBluescript (A600 > 2 U/mL). Following the purification process, a yield of more than 20 µg is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid DNA (1 µg) is used in EcoRI digestion, and analyzed by electrophoresis.

Item#:
ASIBISAMPLEBLUE

MagBio HighPrep™ Plasmid DNA Plus Kit


Magnetic beads based kit designed to extract high-quality plasmid DNA (including Cosmids, Fosmids, and BACs) from bacterial cultures.


Applications

Plasmid DNA isolation for:

  •     PCR, Real-time PCR
  •     Cloning, genotyping
  •     Sequencing, NGS
  •     Transfection

Benefits

  •     High quality plasmid DNA purification
  •     High yields of both high and low copy number plasmids
  •     Adaptable to various automated liquid handling workstations
  •     No toxic organic solvents

The HighPrep™ Plasmid DNA kit is a magnetic beads-based plasmid purification system for high throughput rapid isolation of both high and low copy number plasmid DNA from bacterial cultures as well as for isolation of BACs, PACs, Cosmids or Fosmids.  The system can easily be automated on many liquid handling workstations, providing robustness and high-quality plasmid DNA for subsequent downstream applications such as restriction digestion, PCR, cloning and fluorescent DNA sequencing.
Item#:
ASHIHPRPPLADNAPK

The RNase-Free DNase I set ensures complete DNA removal from RNA extracted using IBI's RNA extraction kits for use in DNA sensitive downstream applications. This is an optional treatment as IBI's spin column technology yields RNA with the majority of DNA removed. Without using DNase treatment the extracted RNA can be used in downstream applications which are not DNA sensitive. This set can be used for both in column DNase digestion and DNase digestion in solution.

Item#:
ASDNARNAKIT2

Sample Types:

Blood, cultured cells, body fluids, and other animal cells

Sample Size:

5 x 10 cultured animal cells, 1 x 10 bacterial cells, or 300µl of blood
Format:
Spin columns
Operation:
Centrifuge/Vacuum

Binding Capacity:
Up to 60µg
Time:
30 minutes
Applications:

RT-PCR, Real-Time PCR, Northern Blotting, Primer Extension, RNAse Protection Assay, mRNA Selection, cDNA Synthesis
Item#:
ASDNARNAKIT1
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