Combo Includes:
200 μL Taq DNA Polymerase (5 units/μL)
4 x 500 μL vials of dNTP Premix (100 mM each of dATP, dCTP, dGTP, dTTP)

D118-1000

High-performance Taq DNA Polymerase specially purified to produce excellent yields with little or no background. Its outstanding activity and unique thermal DNA amplification properties make it one of the most economic DNA polymerases available.

Special Features

• Robust performance
• Leaves 3'A overhang
• Stable at all storage temperatures
• Best price/quality
• APPLICATIONS
• General PCR
• Genomic analysis
• TA cloning

D116

Ready-to-use, ultra-pure, molecular grade solution made from 100mM each of dATP, dCTP, dGTP, and dTTP stock. The PreMix is designed to reduce "hands-on" time for researchers, thereby lowering the possibility of contamination and error. Furthermore, every lot of dNTP solution is pre-tested for optimal performance. The nucleotides are supplied in sterile, doubly distilled water. Each dNTP has a final concentration of 10mM for a total PreMix dNTP concentration of 40mM.

Special Features

• Ready to use
• Each lot functionally tested
• Purity; at least >99%
• APPLICATIONS
• For RT-PCR, large PCR, and real-time PCR
• Reverse Transcription and mutagenesis

Qamp Mini Thermal Cycler

• Compact in Size: 102 x 136 x 104 mm
• Preprogramed Chip: with unlimited applications
• Touch Start: One Button to Go!
• LCD Display: for customers to define status easily!
• Portable: Only 1 kg

• Quickly Spin down droplets and condensation
• Use before and after thermal cycling to increase PCR yield
• Accepts skirted, Non-Skirt and all standard PCR plates
• Less than 1/4 the size of most plate centrifuges
• 1 year warranty

Mini Plate Spinner Adapter Supports:

• 8 x 0.2ml PCR Strips
• 12 x 0.2ml PCR Strips
• 0.2ml PCR Tubes
• 48 well PCR Plates

Please know that the manufacturer has suspended manufacturing this product indefinitely. 
We suggest purchasing PR1MA PCR Plastics.
Contact your rep or email tech@midsci.com for more information.

Platemax CyclerSeal Sealing Film

• Provides exceptional seal to safeguard samples during transport or storage
• Eliminates well to well contamination and cross-over in PCR applications when used with Axygen's compression mat
• Polypropylene film is wide temperature range compatible, -40° to 104°C
• PCR-TS is designed for ELISA/EIA applications
• PCR-TS-900 is twice as thick (as PCR-TS) and is suitable for water-bath and other difficult PCR applications

• Provides exceptional seal under the widest temperature conditions and reagents
• Wide temperature range compatible, -80° to 104°C
• Ideal for light sensitive samples and PCR or storage
• Uniform adhesive provides optimum well sealing
• PCR-AS-200 is pierceable
• 100 films/pack

• Reduces formation of non-specific products
• Improves results from multiplex reactions
• Essential for low copy number
• Buffer II: potassium/ammonium buffer for multiplex reactions
• Activated at elevated temperatures
• Gives higher specificity and greater yields than standard Taq
• Chemical moiety is attached to the enzyme at the active site and renders it inactive at room temperature; is cleaved during a 15 min. heat activation step
• Prevents mispriming during setup and the first ramp of thermal cycling

This brand is being discontinued, please contact us for other options.

Looking for other alternatives?

Visit our PR1MA Polymerase page for more options!

PR DNA Polymerase, High Fidelity, 2.5 U/µL

• Provides higher fidelity than standard Taq DNA Polymerase
• Produces blunt-ended fragments
• Processes <3 kb with extremely high fidelity

Store at -20°C. For in-vitro laboratory use only

Bullseye PREMIUM PR DNA Polymerase is a thermostable enzyme with proofreading ability, which can be used in primer extension reactions and other molecular biology applications. PR Polymerase exhibits both 5'-3' DNA polymerase activity and 3'-5' proofreading exonuclease activity. It is recommended for applications, which require extremely high fidelity or blunt ending.

Optimal reaction conditions are achieved by using the 10x Ammonium buffer containing MgCl2 provided with the enzyme. 25 mM MgCl2 is also included separately, in case a higher MgCl2 concentration is required for a specific reaction.

Unit Definition
One unit is defined as the amount that incorporates 10 nmoles of dNTPs into acid-precipitable form in 30 minutes at 72°C under standard assay conditions.

10X Ammonium Reaction Buffer
Tris-HCl pH 8.5, (NH4)2SO4, 1% Tween20,
15mM MgCl2

PR Storage Buffer
50 mM Tris-HCl (pH 8.0), 50 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% Glycerol, 0.1% NP40, 0.1% Tween-20.

Quality Control
Endonuclease, exonuclease and priming activities are not detected after 3 hours incubation of 1 mg of pUC19 plasmid DNA and 0.5 µg EcoR I digested lambda phage DNA at 72°C in the presence of 40 units of PR DNA Polymerase.

Suggested Protocol using PR DNA Polymerase
This protocol serves as a guideline. Optimal reaction conditions must be individually determined.

1. Thaw 10X Ammonium Buffer, dNTP mix, and primer solutions. It is important to mix the solutions completely before use to avoid localized concentrations of salts.

2. Prepare a master mix according to Table 1. The master mix typically contains all the components needed for extension except the template DNA.
The optimal MgCl2 concentration should be determined empirically but in most cases a concentration of 1.5 mM, as provided in the 1X Ammonium Buffer, will produce satisfactory results. Table 2 provides the volume of 25 mM MgCl2 to add to the master mix if a higher MgCl2 concentration is required.

Table 1. Reaction components (master mix and template DNA)

Table 2. MgCl2 concentration in a 50µl reaction

3. Mix the master mix thoroughly and dispense appropriate volumes into reaction tubes. Mix gently, e.g., by pipetting the master mix up and down a few times.

4. Add template DNA (0.1-0.5 mg/reaction) to the individual tubes containing the master mix.

5. Program the thermal cycler according to the manufacturer's instructions. PR is a proofreading enzyme and requires an extension time of 1-2 min/kb. For maximum yield and specificity, temperatures and cycling times should be optimized for each new template target or primer pair.

HS Taq Polymerase

5 units/µl

Store at -20°C. For in-vitro laboratory use only

General Description

Bullseye HS Taq DNA Polymerase is a modified form of Bullseye Taq DNA Polymerase, which is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during setup and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended. The result is higher specificity and greater yields when compared to standard DNA polymerases.

Once the reaction reaches optimal activating temperature, the chemical moiety is cleaved during a 15 minute heat activation step, releasing the active HS Taq DNA Polymerase into the reaction.

10X HS Buffer I

Tris-HCl, pH 8.5 (NH4)2S04, 15 mM MgCl2, 1% Tween 20.

10X HS Buffer II

An optimized buffer with a balanced ammonium/potassium concentration. May improve results with more complicated PCR reactions such as multiplex PCR.

Tris-HCl pH 8.7, Balanced KCl/(NH4)2S04, 15 mM MgCl2, 1% Tween 20.

HS Taq Storage Buffer

Enzyme is supplied in 20 mM Tris-HCl pH 8.3, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% Tween 20®, 0.5% NP40, 50% glycerol.

Unit Definition

One unit is defined as the amount that incorporates 10 nmoles of dNTPs into acid-precipitable form in 30 minutes at 72°C under standard assay conditions.

Quality Control

Endonuclease, exonuclease and priming activities are not detected after 3 hours incubation of 1 mg of pUC19 plasmid DNA and 0.5 µg EcoR I digested lambda phage DNA at 72°C in the presence of 40 units of TEMPase DNA Polymerase.

Suggested Protocol using HS Taq DNA Polymerase

This protocol serves as a guideline. Optimal reaction conditions must be individually determined.

• 15 mM MgCl2 is present in the 10X HS Buffer I and II. However, in some applications, more than 1.5mM MgCl2 is needed. For this reason, 25mM MgCl2 is included with the kit. Table 2 provides the volume of 25mM MgCl2 to add to the master mix if a higher MgCl2 concentration is required.

  1. Thaw 10X HS Buffer I or/and 10X HS Buffer II, dNTP mix, primer solutions. It is important to mix the solutions completely before use to avoid localized concentrations of salts.

  2. Prepare a master mix according to Table 1. The master mix typically contains all the components needed for extension except the template DNA.

Table 1. Reaction components (master mix & template DNA)

Table 2. MgCl2 concentration in a 50 µL reaction

Looking for other alternatives?

General Description
Bullseye offers a product series specifically developed for the amplification of GC-rich DNA sequences. The Bullseye HS DNA Polymerase combined with GC Buffer I and GC Buffer II promote excellent amplification results with targets of varying degrees of GC content. Hot Start DNA Polymerase is a modified form of Bullseye Taq DNA Polymerase. A chemical moiety is attached to the enzyme at the active site, which renders the enzyme inactive at room temperature. Thus, during setup and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended. Once the reaction reaches optimal activating temperature, the chemical moiety is cleaved during a 10-15 minutes heat activation step, releasing the active Hot Start DNA Polymerase into the reaction. The GC Buffers in combination with Hot Start DNA Polymerase and the heat activation step result in a high success rate in amplification of DNA targets with high GC content.

Key Features

• Amplification of multiple DNA targets with high GC content
  
• High specificity, sensitivity and product yield
  
• Diminished formation of non-specific product
  
• Detection of low copy number targets

  Kit Components:
  HS DNA Polymerase in Storage Buffer
  5 U/ml Hot Start DNA Polymerase, 20 mM Tris-HCl pH 8.9, 100
  mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% TweenÒ 20,
  0.5% NP40, 50% glycerol.
  4x GC Buffer I
  Optimized buffer components, 6 mM MgCl
  4x GC Buffer II
  Optimized buffer components, 6 mM MgCl
  MgCl
  25 mM MgCl in PCR grade water