5-Layer Polystyrene Cell Culture Flask

ReadyStrain™ II Sterile Cell Straining Kits

Reporter gene based Luminescent assay systems from Perkin Elmer provide a homogeneous assay with 96, 384, and 1536 well formats, no DTT (no smell formulation), Ease of storage (2 to 8°C) and shipping (at RT). Different assay formats allow for varying levels of intensity and half-life depending on your application.

• Choose britelite plus:
• Half-life = 30 min
• Brightest signal = when looking for maximum sensitivity
• Shortest half-life = for continuous processing of samples

• Choose neolite:
• Half-life = 150 min
• High signal intensity, longer lasting signal = good balance
• For both continuous and batch processing of samples
• Best robustness (less dependent on mixing quality)
• Less sensitive to phenol red
• Can be stored 1 month at 22°C

• Choose steadylite plus:
  • Half-life = 240 min
  • Longest signal half-life = for batch processing of samples
  • Super-sensitivity not needed

Perkin Elmer's ATPlite simple and sensitive system is an excellent kit to determine cell number due to proliferation or toxicity. It effectively replaces the MTT assay and can be used instead of the 3H thymidine assays with additional analysis.

• Choose ATPlite (2 steps):
• Half-life> 4 hours
• Longest half - life = for batch processing of samples
• 2 step system with separate lysis and detection steps
• More suited for Academia Choose

• ATPlite 1step:
  • Measure within 30 min
  • Brightest signal (~3x the counts of ATPlite 2Steps) = when looking for maximum sensitivity
  • For continuous processing of samples
  • True 1 step system = More suited to HTS

 

	6016736	6016731	6016739	6016943	6016941	6016947	6016949
Assay Protocol	1-step	1-step	1-step	2-step	2-step	2-step	2-step
Automation Compatible	Yes	Yes	Yes	Yes	Yes	Yes	Yes
Detection Method	Luminescence	Luminescence	Luminescence	Luminescence	Luminescence	Luminescence	Luminescence
Experimental Type	In vitro	In vitro	In vitro	In vitro	In vitro	In vitro	In vitro
One Unit Contains	10 mL	100 mL	1000 mL	300 assays	1000 assays	5000 assays	10000 assays
Product Brand Name	ATPlite	ATPlite	ATPlite	ATPlite	ATPlite	ATPlite	ATPlite
Shipping Condition	Ambient	Ambient	Ambient	Ambient	Ambient	Ambient	Ambient
Unit Size	100 (96-well) / 400 (384-well)	1,000 (96-well) / 4,000 (384-well)	10,000 (96-well) / 40,000 (384-well)	300 (96-well) / 1200 (384-well)	1,000 assay points	5,000 (96-well) / 20,000 (384-well)	10,000 (96-well) / 40,000 (384-well)
Vol Capacity	10 mL	100 mL	1000 mL	N/A	N/A	N/A	N/A

Reporter gene based Luminescent assay systems from Perkin Elmer provide a homogeneous assay with 96, 384, and 1536 well formats, no DTT (no smell formulation), Ease of storage (2 to 8°C) and shipping (at RT). Different assay formats allow for varying levels of intensity and half-life depending on your application.

• Choose britelite plus:
• Half-life = 30 min
• Brightest signal = when looking for maximum sensitivity
• Shortest half-life = for continuous processing of samples

• Choose neolite:
• Half-life = 150 min
• High signal intensity, longer lasting signal = good balance
• For both continuous and batch processing of samples
• Best robustness (less dependent on mixing quality)
• Less sensitive to phenol red
• Can be stored 1 month at 22°C

• Choose steadylite plus:
  • Half-life = 240 min
  • Longest signal half-life = for batch processing of samples
  • Super-sensitivity not needed

Recombinant proteins from MIDSCI are expressed in a Pichia Pastoris (yeast) system which provides many advantages for research. Excellent bio-activity at an exceptional value. IBI recombinant proteins are lyophilized and are shipped at room temperature.

ADVANTAGES

• Extensive line of animal (including mouse & rat) and human proteins.
• All proteins are expressed in a Yeast system providing many advantages.
• 100% of the protein purchased is active and usable.
• No endotoxins produced in a yeast system.
• Yeast expression promotes protein folding making product more like its native host.
• Post-translational modifications promote greater bio-activity.
• Proteins are expressed and purified without HIS-TAGS or any other TAGS.
• Proteins are lyophilized and shipped without a carrier protein.
• Excellent quality and value when compared to other products expressed in E. coli.

APPLICATIONS

• Basic immunology (human)
• Veterinary Immunology
• Vaccine Research
• Cell culture proliferation
• Species focused research for human diseases.

Recombinant proteins from MIDSCI are expressed in a Pichia Pastoris (yeast) system which provides many advantages for research. Excellent bio-activity at an exceptional value. IBI recombinant proteins are lyophilized and are shipped at room temperature.

ADVANTAGES

• Extensive line of animal (including mouse & rat) and human proteins.
• All proteins are expressed in a Yeast system providing many advantages.
• 100% of the protein purchased is active and usable.
• No endotoxins produced in a yeast system.
• Yeast expression promotes protein folding making product more like its native host.
• Post-translational modifications promote greater bio-activity.
• Proteins are expressed and purified without HIS-TAGS or any other TAGS.
• Proteins are lyophilized and shipped without a carrier protein.
• Excellent quality and value when compared to other products expressed in E. coli.

APPLICATIONS

• Basic immunology (human)
• Veterinary Immunology
• Vaccine Research
• Cell culture proliferation
• Species focused research for human diseases.

Recombinant proteins from MIDSCI are expressed in a Pichia Pastoris (yeast) system which provides many advantages for research. Excellent bio-activity at an exceptional value. IBI recombinant proteins are lyophilized and are shipped at room temperature.

ADVANTAGES

• Extensive line of animal (including mouse & rat) and human proteins.
• All proteins are expressed in a Yeast system providing many advantages.
• 100% of the protein purchased is active and usable.
• No endotoxins produced in a yeast system.
• Yeast expression promotes protein folding making product more like its native host.
• Post-translational modifications promote greater bio-activity.
• Proteins are expressed and purified without HIS-TAGS or any other TAGS.
• Proteins are lyophilized and shipped without a carrier protein.
• Excellent quality and value when compared to other products expressed in E. coli.

APPLICATIONS

• Basic immunology (human)
• Veterinary Immunology
• Vaccine Research
• Cell culture proliferation
• Species focused research for human diseases.

NEST BioFactory™

• Temperature Range: Approx. -86° to +64°C
• Sterilized by E-Beam SAL=10-6
• Nonpyrogenic
• Double-layer sterile bag
• TC treated, good for cell attachment and growth
• Clear lot number for batch traceability
• Ultrasonic welding without extrinsic ingredients. Excellent welding line design can avoid producing particles during welding. And make the BioFactory™ stronger.
• Vent caps with 0.22um hydrophobic filters for gas exchange without contamination.
• Wide mouth is applicable to pour culture medium directly. Narrow mouth is applicable to operate with the aseptic pipeline.
• Growth kinetics are the same as cells grow in the cell culture flasks. Applicable to large-scale culture adherent cells.
• Large growing area. Just a single operate, you can cultivate a large number of cells and reduce contamination risk.
• Applicable to automatic machine. Standardize operations to reduce batch differences.

Nest BioFactory™ Systems are compact, multi-layer, single-use cell culture systems designed for easy scale-up cell culture applications, such as production of vaccines, monoclonal antibodies, or pharmaceuticals. Versatile, easy-to-use systems for small scale research or commercial production with reduced contamination risk. Narrow mouth caps available for tubing solutions.

BioFactory™ Layer choices

• 1 Layer
• 2 Layers
• 5 Layers
• 10 Layers
• 40 Layers

Material

USP Class VI grade polystyrene material

Harvest is the first product of its kind, providing all the necessary parts for filtration of cell suspensions and combining them into a single, preassembled, ready to use, sterile packet. The kits eliminate the contamination risks associated with opening multiple packages, and assembling separate pieces. The individual sterile packets mean researchers are no longer forced to expose entire bags of 50mL tubes and corresponding caps to perform one filtration. Instead they can open a single ReadyStrain packet directly under the hood.
  

• Kit Includes: strainer, 50 mL tube & cap
• Opens directly under the hood reducing risk of contamination
• Pre-assembled - ready to use
• Guaranteed sterile - gamma
• Packaged in easy-open "tear-strip" bags

NEST Cell Culture Plates

• Sterilized by E-beam
• Vacuum Plasma tissue culture treatment
• Clear lot number for batch traceability
• Peel-to-open, individually wrapped
• Markings of well coordinates available for 12, 24, 48 96 and 384-well plates
• Non-Pyrogenic, DNase/RNase Free
• The package box of Nest cell culture plates can be used as reservoirs

Material
High clarity, 100% virgin polystyrene