Research Essentials 2024 Promo!

For a limited time, buy 2 Erlenmeyer Flasks from this page and get one Free!

Free Flask must be of equal or lesser value; enter Item # plus “-VF” for free product. See flyer for details.
 

All promotions are valid while supplies last. Prices are subject to change without notice.

Valid 09/01/24 – 12/31/24. Use Promo Code #RE2024

Erlenmeyer Flasks

Erlenmeyer Shaker Flasks are made of polycarbonate, PETG or styrene/butadiene (Jumbo Flasks only). The polypropylene screw caps are vented with 0.2µm membranes to increase air transmission and enhance cell growth. The mouth size conforms with the international SBS standard, for quick, secure connection to bioreactor equipment.

Polycarbonate flasks and Jumbo Fernbach flasks have baffled (ribbed) bottoms to provide increased oxygenation and mixing efficiency even at lower shaking speeds. All flasks, regardless of size, are individually wrapped and gamma sterilized to SAL 10-6, and they are free of RNase, DNase, pyrogen, endotoxins and human DNA.

Polycarbonate Flasks are autoclavable at 121°C and 15PSI for 30 minutes.

• Vented screw caps
• Flat or baffled bottoms
• Gamma sterile (SAL 10-6)
• Pyrogen-free and RNase/DNase free
• Individually wrapped 

NEST Cell Culture Inserts

• Innovative hanging design facilitates pipetting.
• Supplied in multiple well plates: 6-well, 12-well, 24well.
• PC membrane: low adsorption rate, reducing the loss of small molecular proteins and other compounds
• Transparent PET inserts permit sufficient optical transparency for visualization of cell outlines by phase contrast microscopy.
• Transparent PET membrane is easy to observe.
• The PC membrane is not easy to be torn or curled, and it is easy to operate when taking out the chamber.
• Compatible with most solvents used for fixing and dyeing.

Attention

• Attachment of cells grown on a permeable support (membrane) is sensitive to the initial seeding density, so the initial use should be inoculated with different density guarantee optimal growth;
• During the cultivation process, the liquid should be taken through the gap between the upper layer and the hole. When adding liquid, be careful and slow to avoid the damage of the membrane;
• Incubation of the permeable support in the medium prior to culturing the cells can improve cell attachment and distribution.

Guidelines

• Pre-equilibration: After added culture solution to the pores of the multi-well plate and inserted the insert, please placed it in the incubator for at least one hour or overnight.
• Fix and stain cells directly in the nest, and use a scalpel to cut the membrane for long-term storage. Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.
• Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.
• Pre-equilibration: After added culture solution to the pores of the multi-well plate and inserted the insert, please placed it in the incubator for at least one hour or overnight.
• Check the volume of the culture medium regularly and add fresh medium as needed.
• The cell layer can be directly fixed and stained in the nest using basic cytological methods. Note Avoid using solvents that dissolve the PC film.
• Fix and stain cells directly in the nest, and use a scalpel to cut the membrane for long-term storage.
• Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.

Material

Multiple well plate or dish is made from PS.

Application

Permeable supports, also known as cell culture inserts, are an essential tool for the study of both anchorage-dependent and independent cell lines.

This product produces a cell culture environment that closely resembles an in vivo state. Allow polarized cells to carry out metabolic activities in a more natural manner because the cells feed both apically and basolaterally.

Application: co-culture, epithelial cell polarity, migration, invasion, transport and permeability studies.

NEST Transfer Cap for High Efficiency Erlenmeyer Flasks

Two-Way Liquid Transfer Cap
The two-way transfer cap is used along with a 2L, 3L or 5L shake flask to connect a liquid inlet tubing with the required device. The liquid transfer is achieved by connection of a peristaltic pump between the shake flask and the device. Upon completion of the transfer, the transfer cap can be replaced with a vent cap for culture.

Multifunctional Liquid Transfer Cap
Unlike the two-way liquid transfer cap, the multifunctional transfer cap can be directly placed in an incubator for culture after the liquid transfer is completed. It can reach a large air flux. The sampling part is composed of a sampling nozzle and a one-way valve, which can prevent the liquid from flowing backwards during the sampling process and ensure the aseptic sampling. The liquid inlet tubing is provided with a PTFE needle filter, which solves the issue of liquid remaining in the tubing during the feeding process.

Inverted Liquid Transfer Cap
The inverted transfer cap is used along with a 2L, 3L or 5L shake flask to connect a liquid inlet tubing with the required device. When liquid transfer is required, the liquid is transferred under gravity with the inverted shake flask.

Application

It is applicable to liquid transfer and culture during mass proliferation of bacteria and suspension cells.

Product Features

• Closed transfer reduces the risks of contamination during liquid transfer
• The cap is molded and connected in an integrated manner, reducing the risks of leakage and media residues
• A variety of tubing diameters are available and aseptic welding of liquid inlet tubing under normal conditions is supported
• High-quality materials and smooth inner wall of the tubing provide an excellent transfer performance
• Sterility level (SAL) is maintained at 10-6
• Endotoxin-free, and no ingredients of animal origin

10 Layer BioFactory™ Culture Chambers with Tube 

Reporter gene based Luminescent assay systems from Perkin Elmer provide a homogeneous assay with 96, 384, and 1536 well formats, no DTT (no smell formulation), Ease of storage (2 to 8°C) and shipping (at RT). Different assay formats allow for varying levels of intensity and half-life depending on your application.

• Choose britelite plus:
• Half-life = 30 min
• Brightest signal = when looking for maximum sensitivity
• Shortest half-life = for continuous processing of samples

• Choose neolite:
• Half-life = 150 min
• High signal intensity, longer lasting signal = good balance
• For both continuous and batch processing of samples
• Best robustness (less dependent on mixing quality)
• Less sensitive to phenol red
• Can be stored 1 month at 22°C

• Choose steadylite plus:
  • Half-life = 240 min
  • Longest signal half-life = for batch processing of samples
  • Super-sensitivity not needed

5-Layer Polystyrene Cell Culture Flask

ReadyStrain™ II Sterile Cell Straining Kits

Reporter gene based Luminescent assay systems from Perkin Elmer provide a homogeneous assay with 96, 384, and 1536 well formats, no DTT (no smell formulation), Ease of storage (2 to 8°C) and shipping (at RT). Different assay formats allow for varying levels of intensity and half-life depending on your application.

• Choose britelite plus:
• Half-life = 30 min
• Brightest signal = when looking for maximum sensitivity
• Shortest half-life = for continuous processing of samples

• Choose neolite:
• Half-life = 150 min
• High signal intensity, longer lasting signal = good balance
• For both continuous and batch processing of samples
• Best robustness (less dependent on mixing quality)
• Less sensitive to phenol red
• Can be stored 1 month at 22°C

• Choose steadylite plus:
  • Half-life = 240 min
  • Longest signal half-life = for batch processing of samples
  • Super-sensitivity not needed

Perkin Elmer's ATPlite simple and sensitive system is an excellent kit to determine cell number due to proliferation or toxicity. It effectively replaces the MTT assay and can be used instead of the 3H thymidine assays with additional analysis.

• Choose ATPlite (2 steps):
• Half-life> 4 hours
• Longest half - life = for batch processing of samples
• 2 step system with separate lysis and detection steps
• More suited for Academia Choose

• ATPlite 1step:
  • Measure within 30 min
  • Brightest signal (~3x the counts of ATPlite 2Steps) = when looking for maximum sensitivity
  • For continuous processing of samples
  • True 1 step system = More suited to HTS

 

	6016736	6016731	6016739	6016943	6016941	6016947	6016949
Assay Protocol	1-step	1-step	1-step	2-step	2-step	2-step	2-step
Automation Compatible	Yes	Yes	Yes	Yes	Yes	Yes	Yes
Detection Method	Luminescence	Luminescence	Luminescence	Luminescence	Luminescence	Luminescence	Luminescence
Experimental Type	In vitro	In vitro	In vitro	In vitro	In vitro	In vitro	In vitro
One Unit Contains	10 mL	100 mL	1000 mL	300 assays	1000 assays	5000 assays	10000 assays
Product Brand Name	ATPlite	ATPlite	ATPlite	ATPlite	ATPlite	ATPlite	ATPlite
Shipping Condition	Ambient	Ambient	Ambient	Ambient	Ambient	Ambient	Ambient
Unit Size	100 (96-well) / 400 (384-well)	1,000 (96-well) / 4,000 (384-well)	10,000 (96-well) / 40,000 (384-well)	300 (96-well) / 1200 (384-well)	1,000 assay points	5,000 (96-well) / 20,000 (384-well)	10,000 (96-well) / 40,000 (384-well)
Vol Capacity	10 mL	100 mL	1000 mL	N/A	N/A	N/A	N/A

Reporter gene based Luminescent assay systems from Perkin Elmer provide a homogeneous assay with 96, 384, and 1536 well formats, no DTT (no smell formulation), Ease of storage (2 to 8°C) and shipping (at RT). Different assay formats allow for varying levels of intensity and half-life depending on your application.

• Choose britelite plus:
• Half-life = 30 min
• Brightest signal = when looking for maximum sensitivity
• Shortest half-life = for continuous processing of samples

• Choose neolite:
• Half-life = 150 min
• High signal intensity, longer lasting signal = good balance
• For both continuous and batch processing of samples
• Best robustness (less dependent on mixing quality)
• Less sensitive to phenol red
• Can be stored 1 month at 22°C

• Choose steadylite plus:
  • Half-life = 240 min
  • Longest signal half-life = for batch processing of samples
  • Super-sensitivity not needed

Recombinant proteins from MIDSCI are expressed in a Pichia Pastoris (yeast) system which provides many advantages for research. Excellent bio-activity at an exceptional value. IBI recombinant proteins are lyophilized and are shipped at room temperature.

ADVANTAGES

• Extensive line of animal (including mouse & rat) and human proteins.
• All proteins are expressed in a Yeast system providing many advantages.
• 100% of the protein purchased is active and usable.
• No endotoxins produced in a yeast system.
• Yeast expression promotes protein folding making product more like its native host.
• Post-translational modifications promote greater bio-activity.
• Proteins are expressed and purified without HIS-TAGS or any other TAGS.
• Proteins are lyophilized and shipped without a carrier protein.
• Excellent quality and value when compared to other products expressed in E. coli.

APPLICATIONS

• Basic immunology (human)
• Veterinary Immunology
• Vaccine Research
• Cell culture proliferation
• Species focused research for human diseases.

Recombinant proteins from MIDSCI are expressed in a Pichia Pastoris (yeast) system which provides many advantages for research. Excellent bio-activity at an exceptional value. IBI recombinant proteins are lyophilized and are shipped at room temperature.

ADVANTAGES

• Extensive line of animal (including mouse & rat) and human proteins.
• All proteins are expressed in a Yeast system providing many advantages.
• 100% of the protein purchased is active and usable.
• No endotoxins produced in a yeast system.
• Yeast expression promotes protein folding making product more like its native host.
• Post-translational modifications promote greater bio-activity.
• Proteins are expressed and purified without HIS-TAGS or any other TAGS.
• Proteins are lyophilized and shipped without a carrier protein.
• Excellent quality and value when compared to other products expressed in E. coli.

APPLICATIONS

• Basic immunology (human)
• Veterinary Immunology
• Vaccine Research
• Cell culture proliferation
• Species focused research for human diseases.