PR1MA™ Tissue Culture Treated Roller Bottles

• Expanded surface roller bottle increases surface area for cell culture

• Large knurled cap for easy grip

• Vented (HRBV) or non-vented cap (HRBNV) for open or closed systems

• Manufactured from medical grade virgin polystyrene

• Tissue Culture Treated Roller Bottles have uniform hydrophilic surface ideal for consistent cell attachment

• Easy-to-read black printed graduations

• Packaged sterile in individual bags

• Vent caps (RBCAP) feature 0.22µm hydrophobic membrane

PR1MA™ Non-Treated Roller Bottles

• Available in two sizes: 1000 mL Vol with 490 cm² of surface area and 2000 mL Vol with 850 cm² of surface area, treated or non-treated versions

• Large, knurled cap for easy grip

• Vented or non-vented cap for open or closed systems

• Manufactured from medical grade virgin polystyrene

• Tissue Culture Treated Roller Bottles have uniform hydrophilic surface ideal for consistent cell attachment

• Easy-to-read black printed graduations

• Packaged sterile (gamma irradiated) in individual bags

Specifications 

Harvest Fetal Bovine Serum (FBS)

Fetal Bovine Serum is collected entirely from USDA-licensed abattoirs and processed within the United States.

• High-quality FBS at an affordable price
• Store at -20ºC 
• Low Endotoxin lots (< 1.0 EU / mL) may be available. 
  
• From USDA-licensed facilities inside and outside the U.S.
• Tested for Growth Promotion*
• Large lot sizes
• Virus Screened-Mycoplasma Tested
• Triple 0.1 uM Filtered
• Endotoxin <  2.0 EU / mL
• Low Hemoglobin lots (< 15 mg / dL) may be available.

Specifications

Proudly made in the USA! Click here for more made in America products!
  

Special Pricing on select filters for a limited time!

Item # RJP3222SH – 100/pk – Was $158.00 - Now $125

 

Offer valid until 12/31/25 or while supplies last. No substitutions.
Cannot be combined with other offers or discounts.
All terms and conditions apply.

 

See Water Systems flyer for more details.

RephiQuik 0.22 uM Syringe Filters

PTFE, PVDF, PES & Nylon Membranes – Non-Sterile.

RephiQuik 0.22 uM Non-sterile Syringe Filters are precision-engineered for fine filtration and sterilization of aqueous, organic, or protein-based solutions in laboratory workflows. Designed for high performance with low extractables, these filters feature four membrane options—PTFE, PVDF, PES, and Nylon—to meet the diverse needs of analytical and research applications.

Each filter is constructed with a durable polypropylene (PP) housing and includes a female luer-lock inlet with a male luer-slip outlet for secure, leak-free operation. The 0.22 uM pore size enables efficient removal of bacteria and fine particulates, ensuring clarity, sterility, and protection of downstream systems such as HPLC instruments and cell cultures.

The filters offer a large effective filtration area with minimal hold-up volume, ensuring maximum recovery of precious samples. The membrane type and pore size are clearly printed on each unit, and color-coded housings provide quick visual identification: blue (PTFE), yellow (PVDF), green (PES), and purple (Nylon).

Membrane Options & Applications

• PTFE (Polytetrafluoroethylene)
• Hydrophobic membrane for highly aggressive solvents, strong acids, and gases. Excellent for chemical compatibility in HPLC and air filtration.
• PVDF (Polyvinylidene fluoride)
• Hydrophilic, low protein binding. Ideal for filtration of protein solutions, biological samples, and molecular biology workflows.
• PES (Polyethersulfone)
• Hydrophilic with asymmetric structure for rapid filtration of aqueous and slightly organic solutions. Optimized for cell culture and sterilization of buffers.
• Nylon
• Hydrophilic, chemically resistant to a wide range of solvents including ACN, DMF, and DMSO. Suitable for aqueous and organic sample filtration. 

Key Features

• Pore Size: 0.22 uM – ideal for sterilizing filtration
• Diameters Available: 13 mm (for up to 10 mL samples) and 32 mm (for up to 100 mL samples)
• Maximum Operating Pressure: 10 bar (145 psi)
• Low hold-up volume with high flow rate
• Leak-proof design for consistent results
• Color-coded for easy membrane identification
• Clearly labeled with membrane type and pore size
• Non-sterile – ready for general lab use and pre-sterilization protocols

Specifications

Diameters	13 mm	32 mm
Effective Filtration Area	0.80 cm2	4.4 cm2
Length, Inlet to outlet	21 mm	26 mm
Housing material	Polypropylene (PP)	Polypropylene (PP)
Inlet Fittings	Female Luer-Lok	Female Luer-Lok
Outlet Fittings	Male Luer slip	Male Luer slip
Process Vol.	1 to 10 mL	10 to 100 mL
Max. Operating Pressure	10 bar (145 psi)	10 bar (145 psi)
Max. Operating Temp.	45ºC	45ºC

 

RephiQuik 0.45 uM Syringe Filters

PTFE, PVDF, PES & Nylon Membranes - Non-sterile.

RephiQuik 0.45 uM Non-sterile Syringe Filters are designed for fast, reliable filtration of aqueous, organic, or protein-based solutions in laboratory settings. Available with PTFE, PVDF, PES, and Nylon membranes, these syringe filters provide broad chemical compatibility, high flow rates, and low extractables—making them ideal for a wide range of filtration applications, including sample preparation, HPLC, and protein purification

Each filter features a high-quality polypropylene (PP) housing and a secure female luer-lock inlet with a male luer-slip outlet. The filters offer a large effective filtration area with minimal hold-up volume, ensuring maximum recovery of precious samples. The membrane type and pore size are clearly printed on each unit, and color-coded housings provide quick visual identification: blue (PTFE), yellow (PVDF), green (PES), and purple (Nylon).

With a consistent pore size of 0.45 uM, these filters support efficient removal of particles and microbes, enabling improved sample clarity and system protection.

Membrane Options & Applications

• PTFE (Polytetrafluoroethylene)
• Hydrophobic, ideal for aggressive organic solvents, acids, and gases. Excellent chemical resistance.
• PVDF (Polyvinylidene fluoride)
• Hydrophilic, low protein binding. Best for protein solutions, biological samples, and DNA/RNA filtration.
• PES (Polyethersulfone)
• Hydrophilic, asymmetric structure for fast flow rates. Recommended for aqueous and slightly organic samples.
• Nylon
• Hydrophilic with broad chemical compatibility. Suitable for both aqueous and organic solvents, including ACN, DMF, and DMSO.

Key Features

• Pore Size: 0.45 uM
• Diameters Available: 13 mm (up to 10 mL samples) and 32 mm (up to 100 mL samples)
• Maximum Operating Pressure: 10 bar (145 psi)
• Low hold-up volume with high throughput
• Leak-proof design to protect downstream analysis
• Color-coded housings for easy identification
• Clearly labeled with pore size and membrane type
• Non-sterile – designed for general laboratory use

Specifications

Diameters	13 mm	32 mm
Effective Filtration Area	0.80 cm2	4.4 cm2
Length, Inlet to outlet	21 mm	26 mm
Housing material	Polypropylene (PP)	Polypropylene (PP)
Inlet Fittings	Female Luer-Lok	Female Luer-Lok
Outlet Fittings	Male Luer slip	Male Luer slip
Process Vol.	1 to 10 mL	10 to 100 mL
Max. Operating Pressure	10 bar (145 psi)	10 bar (145 psi)
Max. Operating Temp.	45ºC	45ºC

 

Special Pricing on select filters for a limited time!

Item # RJF502210 – 10/pk - Was $136.63 - Now $100

 

Offer valid until 12/31/25 or while supplies last. No substitutions.
Cannot be combined with other offers or discounts.
All terms and conditions apply.

 

See Water Systems flyer for more details.

RephiQuik 50 mm PTFE Filter

Hydrophobic Vent Filter for Sterile Gas and Organic Solution Filtration.

The RephiQuik 50 mm PTFE Filter is a high-performance, ready-to-connect venting filter engineered with a hydrophobic PTFE membrane. Designed for use in lab and biopharma environments, this filter ensures safe and sterile air or gas flow, effectively preventing contamination.

Ideal for fermenter air sterilization and incubator gas filtration, the RephiQuik PTFE filter offers exceptional chemical resistance, making it perfectly suited for handling aggressive organic solutions. It can also be used between a pump and a receiving vessel to protect valves and pump components from damage caused by aqueous solutions.

Key Features

• Hydrophobic PTFE membrane supported by high-density polyethylene (HDPE)
• Durable polypropylene housing
• 50 mm diameter for optimized flow
• Excellent chemical compatibility
• Allows free passage of air and gases while blocking aqueous solutions and aerosols
• Equipped with ¼ In. single hose barb connections on both ends
• Autoclavable for repeated sterile use

Applications

• Sterile filtration of fermenter air
• Incubator gas filtration
• Filtration of aggressive organic solvents
• Pump and component protection in fluid systems 

Specifications
 

Diameters	50 mm
Housing material	Polypropylene (PP)
Filter Media material	PTFE
Inlet / Outlet Connections	1/4 In. Hose Barb
Pore Size	0.22 uM

NEST PETG Erlenmeyer Flasks

Glassware performance in a contamination-free, single-use shaker flask. Manufactured from medical-grade virgin PETG which reduces gas permeability.

Nest PETG Erlenmeyer Flasks are designed for mammalian suspension culture applications as well as bacterial & yeast culture.

Seal caps prevent contamination and evaporation. Ideal for long-term storage and transporting liquids. Vent caps with 0.22 um hydrophobic membrane allow gas exchange without contamination (an oversized vent area in the cap improves gas exchange). Ideal for cell culture, fermentation, air-sensitive reactions, and preventing bacterial growth.

• PETG
• Individually packaged in sterile bags
• Sterilized by E-Beam
• DNase/RNase free, no pyrogens   

NEST PC Conical Erlenmeyer Flasks

Glassware performance in a contamination-free, single-use shaker flask. Polycarbonate bottle meets the USP Class 6 Standard with high transparency, strong impact resistance and oxidation resistance, and can withstand temperatures up to 121ºC. There’s a scale made by injection molding on the flask body to facilitate volume observation.

Nest Polycarbonate Conical Erlenmeyer Flasks are designed for mammalian suspension culture applications as well as bacterial & yeast culture.

Seal caps prevent contamination and evaporation. Ideal for long-term storage and transporting liquids.

Vent caps with 0.22 um hydrophobic membrane allow gas exchange without contamination (an oversized vent area in the cap improves gas exchange). Ideal for cell culture, fermentation, air-sensitive reactions, and preventing bacterial growth.

• Polycarbonate
• DNase/RNase free, no pyrogens
• Sterilized by E-Beam
• Individually packaged in sterile bags   

NEST Cell Culture Inserts

• Innovative hanging design facilitates pipetting.
• Supplied in multiple well plates: 6-well, 12-well, 24well.
• PC membrane: low adsorption rate, reducing the loss of small molecular proteins and other compounds
• Transparent PET inserts permit sufficient optical transparency for visualization of cell outlines by phase contrast microscopy.
• Transparent PET membrane is easy to observe.
• The PC membrane is not easy to be torn or curled, and it is easy to operate when taking out the chamber.
• Compatible with most solvents used for fixing and dyeing.

Attention

• Attachment of cells grown on a permeable support (membrane) is sensitive to the initial seeding density, so the initial use should be inoculated with different density guarantee optimal growth;
• During the cultivation process, the liquid should be taken through the gap between the upper layer and the hole. When adding liquid, be careful and slow to avoid the damage of the membrane;
• Incubation of the permeable support in the medium prior to culturing the cells can improve cell attachment and distribution.

Guidelines

• Pre-equilibration: After added culture solution to the pores of the multi-well plate and inserted the insert, please placed it in the incubator for at least one hour or overnight.
• Fix and stain cells directly in the nest, and use a scalpel to cut the membrane for long-term storage. Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.
• Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.
• Pre-equilibration: After added culture solution to the pores of the multi-well plate and inserted the insert, please placed it in the incubator for at least one hour or overnight.
• Check the volume of the culture medium regularly and add fresh medium as needed.
• The cell layer can be directly fixed and stained in the nest using basic cytological methods. Note Avoid using solvents that dissolve the PC film.
• Fix and stain cells directly in the nest, and use a scalpel to cut the membrane for long-term storage.
• Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.

Material

Multiple well plate or dish is made from PS.

Application

Permeable supports, also known as cell culture inserts, are an essential tool for the study of both anchorage-dependent and independent cell lines.

This product produces a cell culture environment that closely resembles an in vivo state. Allow polarized cells to carry out metabolic activities in a more natural manner because the cells feed both apically and basolaterally.

Application: co-culture, epithelial cell polarity, migration, invasion, transport and permeability studies.

NEST Transfer Cap for High Efficiency Erlenmeyer Flasks

Two-Way Liquid Transfer Cap
The two-way transfer cap is used along with a 2L, 3L or 5L shake flask to connect a liquid inlet tubing with the required device. The liquid transfer is achieved by connection of a peristaltic pump between the shake flask and the device. Upon completion of the transfer, the transfer cap can be replaced with a vent cap for culture.

Multifunctional Liquid Transfer Cap
Unlike the two-way liquid transfer cap, the multifunctional transfer cap can be directly placed in an incubator for culture after the liquid transfer is completed. It can reach a large air flux. The sampling part is composed of a sampling nozzle and a one-way valve, which can prevent the liquid from flowing backwards during the sampling process and ensure the aseptic sampling. The liquid inlet tubing is provided with a PTFE needle filter, which solves the issue of liquid remaining in the tubing during the feeding process.

Inverted Liquid Transfer Cap
The inverted transfer cap is used along with a 2L, 3L or 5L shake flask to connect a liquid inlet tubing with the required device. When liquid transfer is required, the liquid is transferred under gravity with the inverted shake flask.

Application

It is applicable to liquid transfer and culture during mass proliferation of bacteria and suspension cells.

Product Features

• Closed transfer reduces the risks of contamination during liquid transfer
• The cap is molded and connected in an integrated manner, reducing the risks of leakage and media residues
• A variety of tubing diameters are available and aseptic welding of liquid inlet tubing under normal conditions is supported
• High-quality materials and smooth inner wall of the tubing provide an excellent transfer performance
• Sterility level (SAL) is maintained at 10-6
• Endotoxin-free, and no ingredients of animal origin

10 Layer BioFactory™ Culture Chambers with Tube 

Reporter gene based Luminescent assay systems from Perkin Elmer provide a homogeneous assay with 96, 384, and 1536 well formats, no DTT (no smell formulation), Ease of storage (2 to 8°C) and shipping (at RT). Different assay formats allow for varying levels of intensity and half-life depending on your application.

• Choose britelite plus:
• Half-life = 30 min
• Brightest signal = when looking for maximum sensitivity
• Shortest half-life = for continuous processing of samples

• Choose neolite:
• Half-life = 150 min
• High signal intensity, longer lasting signal = good balance
• For both continuous and batch processing of samples
• Best robustness (less dependent on mixing quality)
• Less sensitive to phenol red
• Can be stored 1 month at 22°C

• Choose steadylite plus:
  • Half-life = 240 min
  • Longest signal half-life = for batch processing of samples
  • Super-sensitivity not needed