PR1MA™ Plates-Permeable Cell Culture Inserts

• Permeable Cell Culture Inserts mimic in vivo conditions
• Inserts center in wells, preventing membrane from touching sides and wicking between insert and side wall
• Membrane remains suspended above well bottom, allowing co-culture: grow one cell line inside insert, and another below in the well
• Ideal for drug transfer assays, transport studies, migration assays, and cell-cell interaction studies
• Transfer cultures to fresh media by moving the insert to a new plate
• 0.4 µm pore size is ideal for co-culture and drug transport studies
• 3.0 µm and 8.0 µm pore size are ideal for angiogenesis and chemotaxis experiments
• For more help with membrane selection, please contact CELLTREAT Scientific Products
• Non-Treated Plates sold separately; carrier plate is Tissue Culture Treated which encourages cell adhesion

Specifications 

NEST Cell Culture Inserts

• Innovative hanging design facilitates pipetting.
• Supplied in multiple well plates: 6-well, 12-well, 24well.
• PC membrane: low adsorption rate, reducing the loss of small molecular proteins and other compounds
• Transparent PET inserts permit sufficient optical transparency for visualization of cell outlines by phase contrast microscopy.
• Transparent PET membrane is easy to observe.
• The PC membrane is not easy to be torn or curled, and it is easy to operate when taking out the chamber.
• Compatible with most solvents used for fixing and dyeing.

Attention

• Attachment of cells grown on a permeable support (membrane) is sensitive to the initial seeding density, so the initial use should be inoculated with different density guarantee optimal growth;
• During the cultivation process, the liquid should be taken through the gap between the upper layer and the hole. When adding liquid, be careful and slow to avoid the damage of the membrane;
• Incubation of the permeable support in the medium prior to culturing the cells can improve cell attachment and distribution.

Guidelines

• Pre-equilibration: After added culture solution to the pores of the multi-well plate and inserted the insert, please placed it in the incubator for at least one hour or overnight.
• Fix and stain cells directly in the nest, and use a scalpel to cut the membrane for long-term storage. Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.
• Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.
• Pre-equilibration: After added culture solution to the pores of the multi-well plate and inserted the insert, please placed it in the incubator for at least one hour or overnight.
• Check the volume of the culture medium regularly and add fresh medium as needed.
• The cell layer can be directly fixed and stained in the nest using basic cytological methods. Note Avoid using solvents that dissolve the PC film.
• Fix and stain cells directly in the nest, and use a scalpel to cut the membrane for long-term storage.
• Before using please add culture solution to the pores of the multi-well plate, then insert the insert and add the culture solution which included cells to the insert.

Material

Multiple well plate or dish is made from PS.

Application

Permeable supports, also known as cell culture inserts, are an essential tool for the study of both anchorage-dependent and independent cell lines.

This product produces a cell culture environment that closely resembles an in vivo state. Allow polarized cells to carry out metabolic activities in a more natural manner because the cells feed both apically and basolaterally.

Application: co-culture, epithelial cell polarity, migration, invasion, transport and permeability studies.