Harvest is the first product of its kind, providing all the necessary parts for filtration of cell suspensions and combining them into a single, preassembled, ready to use, sterile packet. The kits eliminate the contamination risks associated with opening multiple packages, and assembling separate pieces. The individual sterile packets mean researchers are no longer forced to expose entire bags of 50mL tubes and corresponding caps to perform one filtration. Instead they can open a single ReadyStrain packet directly under the hood.
  

• Kit Includes: strainer, 50 mL tube & cap
• Opens directly under the hood reducing risk of contamination
• Pre-assembled - ready to use
• Guaranteed sterile - gamma
• Packaged in easy-open "tear-strip" bags

For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

VitroINK® – Bioink Starter Kits
 
VitroINK® (3 mL) + Full Mixing Kit with Dispenser

VitroINK® Bioink Starter Kit contains both bioink and the complete mixing kit to prepare the cell bioink mixture for 3D bioprinting. The kit includes choice of bioink + the mixing kit:

• VitroINK 3D (3 mL) + VitroINK® Mixing Kit with Dispenser
• VitroINK RGD (3 mL) + VitroINK® Mixing Kit with Dispenser
• VitroINK COL (3 mL) + VitroINK® Mixing Kit with Dispenser
• VitroINK MMP (3 mL) + VitroINK® Mixing Kit with Dispenser
• VitroINK IKVAV (3 mL) + VitroINK® Mixing Kit with Dispenser
• VitroINK YIGSR (3 mL) + VitroINK® Mixing Kit with Dispenser

The VitroINK® Mixing Kit includes a dispenser, 1 mL syringe, 3 mL syringe, connectors and mixing head to get you started.

VitroINK® is a ready-to-use tunable bioink modified with functional ligands, and cell adhesive peptide, promoting cell attachment and cell-matrix interactions. This RGD modified bioink can enhance cell adhesion, proliferation, motility/migration, and differentiation in different applications.

VitroINK® Mixing Kit was designed to provide a robust mixing of bioink and cells. Cells can be prepared with VitroINK® for a 3:1 mixing ratio using the 3 mL syringe or a 10:1 mixing ratio using the 1 mL syringe. Put the VitroINK® and cell suspension in the mixer and gently press the mixture through the connector and mixing head to your bioprinter cartridge. Wait 10-20 minutes for the mixture to stabilize before printing. There is no UV,  no temperature/pH curing, or chemical cross-linking for the VitroINK® system. Simply adding cell culture medium to cover the printed structure can further stabilize and support cell growth. The bioprinted cells are ready for incubation.

Contents and Specifications

VitroINK®

• Xeno-free tunable modified bioink.
• Ready-to-use at room temperature
• No UV, temperature/pH curing, or chemical cross-linking required
• Neutral pH
• Transparent. Excellent visibility after printing and cell culture
• Pre-mix with cells using the included VitroINK® Mixing Kit
• Ships room temperature. Store at 2-8°C
• Size: 3 mL

VitroINK® Mixing Kit

• Complete mixing kit.  Includes:
• (1) 1 mL syringe
• (1) 3 mL syringe
• (1) connector and tubing
• (1) mixing head
• Sterile
• Use for 3:1 or 10:1 mixing ratio
• Size: 3 mL

10 Layer BioFactory™ Culture Chambers with Tube 

Reporter gene based Luminescent assay systems from Perkin Elmer provide a homogeneous assay with 96, 384, and 1536 well formats, no DTT (no smell formulation), Ease of storage (2 to 8°C) and shipping (at RT). Different assay formats allow for varying levels of intensity and half-life depending on your application.

• Choose britelite plus:
• Half-life = 30 min
• Brightest signal = when looking for maximum sensitivity
• Shortest half-life = for continuous processing of samples

• Choose neolite:
• Half-life = 150 min
• High signal intensity, longer lasting signal = good balance
• For both continuous and batch processing of samples
• Best robustness (less dependent on mixing quality)
• Less sensitive to phenol red
• Can be stored 1 month at 22°C

• Choose steadylite plus:
  • Half-life = 240 min
  • Longest signal half-life = for batch processing of samples
  • Super-sensitivity not needed

5-Layer Polystyrene Cell Culture Flask

Reporter gene based Luminescent assay systems from Perkin Elmer provide a homogeneous assay with 96, 384, and 1536 well formats, no DTT (no smell formulation), Ease of storage (2 to 8°C) and shipping (at RT). Different assay formats allow for varying levels of intensity and half-life depending on your application.

• Choose britelite plus:
• Half-life = 30 min
• Brightest signal = when looking for maximum sensitivity
• Shortest half-life = for continuous processing of samples

• Choose neolite:
• Half-life = 150 min
• High signal intensity, longer lasting signal = good balance
• For both continuous and batch processing of samples
• Best robustness (less dependent on mixing quality)
• Less sensitive to phenol red
• Can be stored 1 month at 22°C

• Choose steadylite plus:
  • Half-life = 240 min
  • Longest signal half-life = for batch processing of samples
  • Super-sensitivity not needed

Perkin Elmer's ATPlite simple and sensitive system is an excellent kit to determine cell number due to proliferation or toxicity. It effectively replaces the MTT assay and can be used instead of the 3H thymidine assays with additional analysis.

• Choose ATPlite (2 steps):
• Half-life> 4 hours
• Longest half - life = for batch processing of samples
• 2 step system with separate lysis and detection steps
• More suited for Academia Choose

• ATPlite 1step:
  • Measure within 30 min
  • Brightest signal (~3x the counts of ATPlite 2Steps) = when looking for maximum sensitivity
  • For continuous processing of samples
  • True 1 step system = More suited to HTS

 

	6016736	6016731	6016739	6016943	6016941	6016947	6016949
Assay Protocol	1-step	1-step	1-step	2-step	2-step	2-step	2-step
Automation Compatible	Yes	Yes	Yes	Yes	Yes	Yes	Yes
Detection Method	Luminescence	Luminescence	Luminescence	Luminescence	Luminescence	Luminescence	Luminescence
Experimental Type	In vitro	In vitro	In vitro	In vitro	In vitro	In vitro	In vitro
One Unit Contains	10 mL	100 mL	1000 mL	300 assays	1000 assays	5000 assays	10000 assays
Product Brand Name	ATPlite	ATPlite	ATPlite	ATPlite	ATPlite	ATPlite	ATPlite
Shipping Condition	Ambient	Ambient	Ambient	Ambient	Ambient	Ambient	Ambient
Unit Size	100 (96-well) / 400 (384-well)	1,000 (96-well) / 4,000 (384-well)	10,000 (96-well) / 40,000 (384-well)	300 (96-well) / 1200 (384-well)	1,000 assay points	5,000 (96-well) / 20,000 (384-well)	10,000 (96-well) / 40,000 (384-well)
Vol Capacity	10 mL	100 mL	1000 mL	N/A	N/A	N/A	N/A

Reporter gene based Luminescent assay systems from Perkin Elmer provide a homogeneous assay with 96, 384, and 1536 well formats, no DTT (no smell formulation), Ease of storage (2 to 8°C) and shipping (at RT). Different assay formats allow for varying levels of intensity and half-life depending on your application.

• Choose britelite plus:
• Half-life = 30 min
• Brightest signal = when looking for maximum sensitivity
• Shortest half-life = for continuous processing of samples

• Choose neolite:
• Half-life = 150 min
• High signal intensity, longer lasting signal = good balance
• For both continuous and batch processing of samples
• Best robustness (less dependent on mixing quality)
• Less sensitive to phenol red
• Can be stored 1 month at 22°C

• Choose steadylite plus:
  • Half-life = 240 min
  • Longest signal half-life = for batch processing of samples
  • Super-sensitivity not needed

Recombinant proteins from MIDSCI are expressed in a Pichia Pastoris (yeast) system which provides many advantages for research. Excellent bio-activity at an exceptional value. IBI recombinant proteins are lyophilized and are shipped at room temperature.

ADVANTAGES

• Extensive line of animal (including mouse & rat) and human proteins.
• All proteins are expressed in a Yeast system providing many advantages.
• 100% of the protein purchased is active and usable.
• No endotoxins produced in a yeast system.
• Yeast expression promotes protein folding making product more like its native host.
• Post-translational modifications promote greater bio-activity.
• Proteins are expressed and purified without HIS-TAGS or any other TAGS.
• Proteins are lyophilized and shipped without a carrier protein.
• Excellent quality and value when compared to other products expressed in E. coli.

APPLICATIONS

• Basic immunology (human)
• Veterinary Immunology
• Vaccine Research
• Cell culture proliferation
• Species focused research for human diseases.

Recombinant proteins from MIDSCI are expressed in a Pichia Pastoris (yeast) system which provides many advantages for research. Excellent bio-activity at an exceptional value. IBI recombinant proteins are lyophilized and are shipped at room temperature.

ADVANTAGES

• Extensive line of animal (including mouse & rat) and human proteins.
• All proteins are expressed in a Yeast system providing many advantages.
• 100% of the protein purchased is active and usable.
• No endotoxins produced in a yeast system.
• Yeast expression promotes protein folding making product more like its native host.
• Post-translational modifications promote greater bio-activity.
• Proteins are expressed and purified without HIS-TAGS or any other TAGS.
• Proteins are lyophilized and shipped without a carrier protein.
• Excellent quality and value when compared to other products expressed in E. coli.

APPLICATIONS

• Basic immunology (human)
• Veterinary Immunology
• Vaccine Research
• Cell culture proliferation
• Species focused research for human diseases.

Recombinant proteins from MIDSCI are expressed in a Pichia Pastoris (yeast) system which provides many advantages for research. Excellent bio-activity at an exceptional value. IBI recombinant proteins are lyophilized and are shipped at room temperature.

ADVANTAGES

• Extensive line of animal (including mouse & rat) and human proteins.
• All proteins are expressed in a Yeast system providing many advantages.
• 100% of the protein purchased is active and usable.
• No endotoxins produced in a yeast system.
• Yeast expression promotes protein folding making product more like its native host.
• Post-translational modifications promote greater bio-activity.
• Proteins are expressed and purified without HIS-TAGS or any other TAGS.
• Proteins are lyophilized and shipped without a carrier protein.
• Excellent quality and value when compared to other products expressed in E. coli.

APPLICATIONS

• Basic immunology (human)
• Veterinary Immunology
• Vaccine Research
• Cell culture proliferation
• Species focused research for human diseases.

NEST BioFactory™

• Temperature Range: Approx. -86° to +64°C
• Sterilized by E-Beam SAL=10-6
• Nonpyrogenic
• Double-layer sterile bag
• TC treated, good for cell attachment and growth
• Clear lot number for batch traceability
• Ultrasonic welding without extrinsic ingredients. Excellent welding line design can avoid producing particles during welding. And make the BioFactory™ stronger.
• Vent caps with 0.22um hydrophobic filters for gas exchange without contamination.
• Wide mouth is applicable to pour culture medium directly. Narrow mouth is applicable to operate with the aseptic pipeline.
• Growth kinetics are the same as cells grow in the cell culture flasks. Applicable to large-scale culture adherent cells.
• Large growing area. Just a single operate, you can cultivate a large number of cells and reduce contamination risk.
• Applicable to automatic machine. Standardize operations to reduce batch differences.

Nest BioFactory™ Systems are compact, multi-layer, single-use cell culture systems designed for easy scale-up cell culture applications, such as production of vaccines, monoclonal antibodies, or pharmaceuticals. Versatile, easy-to-use systems for small scale research or commercial production with reduced contamination risk. Narrow mouth caps available for tubing solutions.

BioFactory™ Layer choices

• 1 Layer
• 2 Layers
• 5 Layers
• 10 Layers
• 40 Layers

Material

USP Class VI grade polystyrene material