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MIDSCI has the TPP plastics, equipment, and supplies for all of your cell/tissue culture needs.
Looking to save the hassle of buying individual pieces of cell/tissue culture equipment and supplies? We can get you up and running with our tissue culture start-up bundle in no time at all.
If, instead, you’re simply looking for culture ware, then we’re able to provide you with premium TPP culture ware in no time flat; including flasks, tubes, dishes, and plates.
And if it’s time to get some new cell/tissue culture equipment, we offer high-quality, durable, and (in some cases) compact refrigerated incubators, low-profile rollers, rockers, ovens, and a lot more.
Recombinant proteins from MIDSCI are expressed in a Pichia Pastoris (yeast) system which provides many advantages for research. Excellent bio-activity at an exceptional value. IBI recombinant proteins are lyophilized and are shipped at room temperature.
ADVANTAGES
- Extensive line of animal (including mouse & rat) and human proteins.
- All proteins are expressed in a Yeast system providing many advantages.
- 100% of the protein purchased is active and usable.
- No endotoxins produced in a yeast system.
- Yeast expression promotes protein folding making product more like its native host.
- Post-translational modifications promote greater bio-activity.
- Proteins are expressed and purified without HIS-TAGS or any other TAGS.
- Proteins are lyophilized and shipped without a carrier protein.
- Excellent quality and value when compared to other products expressed in E. coli.
APPLICATIONS
- Basic immunology (human)
- Veterinary Immunology
- Vaccine Research
- Cell culture proliferation
- Species focused research for human diseases.
NEST BioFactory™
- Temperature Range: Approx. -86° to +64°C
- Sterilized by E-Beam SAL=10-6
- Nonpyrogenic
- Double-layer sterile bag
- TC treated, good for cell attachment and growth
- Clear lot number for batch traceability
- Ultrasonic welding without extrinsic ingredients. Excellent welding line design can avoid producing particles during welding. And make the BioFactory™ stronger.
- Vent caps with 0.22um hydrophobic filters for gas exchange without contamination.
- Wide mouth is applicable to pour culture medium directly. Narrow mouth is applicable to operate with the aseptic pipeline.
- Growth kinetics are the same as cells grow in the cell culture flasks. Applicable to large-scale culture adherent cells.
- Large growing area. Just a single operate, you can cultivate a large number of cells and reduce contamination risk.
- Applicable to automatic machine. Standardize operations to reduce batch differences.
Nest BioFactory™ Systems are compact, multi-layer, single-use cell culture
systems designed for easy scale-up cell culture applications, such as
production of vaccines, monoclonal antibodies, or pharmaceuticals.
Versatile, easy-to-use systems for small scale research or commercial
production with reduced contamination risk. Narrow mouth caps available for
tubing solutions.
BioFactory™ Layer choices
- 1 Layer
- 2 Layers
- 5 Layers
- 10 Layers
- 40 Layers
Material
USP Class VI grade polystyrene material
NEST Cell Culture Plates
- Sterilized by E-beam
- Vacuum Plasma tissue culture treatment
- Clear lot number for batch traceability
- Peel-to-open, individually wrapped
- Markings of well coordinates available for 12, 24, 48 96 and 384-well plates
- Non-Pyrogenic, DNase/RNase Free
- The package box of Nest cell culture plates can be used as reservoirs
Material
High clarity, 100% virgin polystyrene
3D Stackable Mini Roller
- Wide range of speed adjustments for a variety of applications
- Anti-corrosive and wear-resistant construction for use in humid and CO2 environments
- Easy to add or remove rollers to fit different-sized tubes or bottles
- A stackable mechanism allows two units to be stacked for space savings
- A remote control box makes it easy to operate the unit while it is in an incubator
Specifications:
| Model | MR-03UA |
| Number of Roller Bars | 11 pcs |
| Speed | 0.5~80 rpm |
| Speed Accuracy | ±1 rpm |
| Roller Dimensions (D×L) | Ø28 x 269 mm (Ø1.1 × 10.6 in.) |
| Swing Dimensions | 18 mm (0.71 in.) |
| Available Tubes or Bottles | Max. Ø120 mm (4.72 in.) bottles/tubes |
| Max. Load | 6.5kg (14.33 lbs) with the container, 3 × 1,500 mL bottles |
| Digital Display | Roller Speed Display |
| Timing Range (Control Box) | 0~999 min or continuous operation |
| Included Accessory Standard in Packaging | MRA02, MRA03, MRA05 |
| Ambient Temperature | 5 ~ 60 |
| Relative Humidity | ≤95% |
| Power Supply | AC110 / 60Hz |
| Max. Power | 10W |
| Dimensions (D×W×H) | 360 x 378 x 72 mm (14.17 x 14.88 x 2.83 in.) |
| Net Weight | 6.5kg (14.33 lbs) |
| Stacking | Optional |
| Protection Level | IP21 |
| Certifications | cCSAus, WEEE, RoHs, CE |
Small Erlenmeyer Flask
- Lightweight plastic material is easier and safer to handle than glass
- Designed for mammalian suspension culture applications as well as bacterial & yeast culture
- Baffled flasks enhance both vertical and circular culture movement to improve aeration
- Vent cap options are available to prevent the common mistake of unintentionally sealing the cap completely and preventing gas exchange
- Polycarbonate available with vent cap only
- Vent caps with 0.22μm hydrophobic membrane feature an oversized vent area for improved gas exchange without contamination
- All flasks include molded graduation lines
- Baffled flasks feature a molded 1/3 fill line to help ensure optimal working volumes
- Individually bagged for easy storage and handling
- Medical grade virgin Polycarbonate is autoclavable (temperature range of -80°C – 121°C)
- Medical grade virgin PETG reduces gas permeability (temperature range of -80°C – 60°C)
- Working volumes are recommendations only; optimize as required for individual protocols
- Designed for mammalian suspension culture applications as well as bacterial & yeast culture
- Vent with over-sized vent area feature 0.22 µm hydrophobic membrane which allows for improved gas exchange without contamination
- Avoid contamination with single-use, pre-sterilized flasks
- Lightweight material is easier to handle than glass
- Flasks include molded graduation lines
- Individually bagged for easy storage and handling
- Medical grade virgin Polycarbonate is autoclavable
Accuris SmartReader™ UV-Vis Microplate Reader
The Accuris SmartReader™ UV-Vis Microplate Reader (model
MR9610) has been designed with ease-of-use and fast results in mind. This
instrument brings extensive capabilities to the lab bench and is designed for
the busy lab with multiple users. The large touch screen command center is
extremely user-friendly and allows for fast set up of new protocols, as well as
modifying and running existing protocols.
- Intuitive, 10-inch color
touch screen control panel
- Accepts both 96 & 384-well
plates
- Monochromator-based
system
• No filters required
• Wavelength range from 200 to 1000 nm - Stand-alone design
• No PC or special software required
• USB port for flash drive data transfer
Monochromator technology … no filters needed
The monochromator-based optical system allows for precise
selection of wavelengths from 200nm to 1000nm, with no filters required. This
range makes the SmartReader UV-Vis™ useful not only for typical ELISA ,
Bradford Lowry, and turbidity assays but also for more advanced applications
such as quantitation of nucleic acids and proteins. The SmartReader™ UV-Vis can
be set for Endpoint, Kinetic and Spectral Scanning assays.
High Throughput Capability
The MR9610 is designed for efficient processing of multiple
samples and
accepts standard 96 well plates as well as 384 well plates for high density
screening. The plate layout interface allows for a quick and intuitive setup of
a plate well’s contents. Users can highlight and label multiple wells to save
time. The display shows a graphical representation of the plate. Users can
label each well as required for: “known standard”, “unknown sample”, “blank
well” , “QC Control well”, “positive control”, “negative control”, and “empty”.
Optimized results with plate shaking and incubation
Controllable plate shaking and incubation allow for optimization of assay results. The shaking speed can be controlled at different intensities with options for timed and pulsed shaking before a reading, or in between readings when performing a kinetic assay. The linear motion efficiently and uniformly mixes all samples in both 96 and 384 well plates. Temperature can be controlled from ambient +5C up to 45C, allowing for the processing of enzyme kinetics, and bacterial growth assays.”
SmartDrop™ Accessory Plate
Absorbance measurements of exceptionally small volumes
(<0.5ul) can be achieved using the SmartDrop™ Accessory Plate. The
SmartDrop™ plate accommodates up to 16 samples and is ideal for checking
nucleic acid concentrations and purity for Next Gen Sequencing, or protein
concentrations.
SmartReader™ UV-Vis Microplate Reader Specifications
|
Model |
Accuris SmartReader™ UV-Vis |
|
Plates Accepted |
96-well and
384-well flat bottom |
|
Display |
10-inch color
touch screen |
|
Controls |
Touch screen
and mouse |
|
Light Source |
Xenon Flash
Lamp |
|
Wavelength range |
200-1000nm |
|
Wavelength Precision |
± 2 nm |
|
Wavelength Accuracy |
± 2 nm |
|
Wavelength selection |
Monochromator |
|
Read-out range |
0 – 4.0 OD |
|
Detector |
Dual silicon photoelectric
detectors for measurement and reference |
|
Linearity @ 450nm |
R2 ≥
0.999, [0.0 – 3.0] |
|
Accuracy @ 450nm |
1.0% + 0.003
Abs (0 – 2.0] |
|
Precision@450nm |
CV < 0.5% or
SD<0.003 (Precision mode); CV < 1.0% (Fast mode) |
|
Stability@450nm |
< 0.005A ,
(0.0 – 2.0] |
|
Measurement speed |
96 well
plate: <6 seconds (fast mode); <25 seconds (precision mode) |
|
Plate shaking |
Linear; three
levels of shaking speed |
|
Incubator |
From ambient +
5°C to 45°C |
|
Temperature Uniformity |
± 0.5°C @ 37°C |
|
Storage |
16G memory,
more than 10,000 test records can be stored |
|
Connection Ports |
3 USB ports,
for PC, printer and USB-flash drive |
|
Power supply |
DC24V
6.5A |
|
Dimensions (WxDxH) |
300 x 500 x 260mm |
11.8 x 19.7 x 10.25 inches |
|
Weight |
15.5kg | 34 Lbs |
Ideal for rapid, accurate counts.
The all-in-one eCount combines a multifunction electronic counter with a Sharpie® pen for marking petri dishes to prevent missing or double-counting colonies. Light, ergonomic body is balanced for easy manipulation. Touch controls for multiple counts, sums, counting up or down, and more! Includes fine-point Sharpie® pen and weighted stand.
- Pressure-sensitive counting
- Average count facility
- Bright white energy saving LED lighting
- With BioCote antimicrobial protection
- Audible confirmation
- Choice of light or dark background
- Connectivity to printer or computer
Touch pressure with felt tip marker on petri dish registers cumulative count on the digital display with confirmation by audible tone (can be turned on or off). The pressure required to register a count can be adjusted to suit each user. Averaging facility calculates average count over multiple plates. Counting results as well as useful statistics including SD can be sent directly to the accessory printer or to a computer via a USB cable supplied.
Sub-stage illumination by low energy bright LED's allows glare-free optimum viewing. A switchable black background is provided to enhance viewing of translucent and difficult to see colonies. Supplied with a Wolffhuegel graticule, segmentation dish and dish centering adapters to facilitate use with 50mm to 90mm dishes. A choice of magnifiers and a printer are available as optional accessories.
Technical Specifications
| Lighting | White LED array |
| Digital display | 3 digit LED |
| Count | 0 to 999 |
| Dimensions (w x d x h), in. | 12 x 12 x 5.5" |
| Mass, lbs | 3.3 |
| Electrical supply | 120 to 230V, 50 / 60Hz, 70W |
Please check out alternative suspension cell culture dishes here.
Sterile and free from optical distortion, these dishes are ideal for phage typing, susceptibility testing, plate counts and probe assays. Each 13mm grid is marked numerically in one direction and alphabetically in the other. This dish has four venting ribs into the underside of the lid to prevent condensation build up.
Made of polystyrene.
Dimensions: 90 X 15 mm
Volume: 110 mL
Qty/sleeve: 10
Qty/case: 500
Aspire Aspirator
- Compact Design
- Built-in Vacuum Pump
- Adjustable Vacuum Pressure
- Vacuum Gauge
- Safe and Precise Aspiration
The Accuris ASPIRE Laboratory Aspirator incorporates a quiet, maintenance-free pump inside a sturdy, yet compact base. The vacuum is fully adjustable, with a pressure gauge that displays the vacuum level, allowing full control of aspirating speed. The ASPIRE is an ideal choice for a wide range of laboratory aspirating applications, including removal of media from tissue culture vessels or plates, and supernatants. An ergonomic, hand held aspirating device is included, and holds standard 200 to 300ul disposable pipette tips.
Comes complete with:
- Base with internal vacuum pump
- 2 Liter, Polycarbonate Collection Bottle (autoclavable and break resistant)
- Antibacterial / Hydrophobic Filter
- Silicone Tubing
- Handheld Vacuum Controller
- 2 Pipette cone adapters (for 200 ul pipette tips and 40mm stainless steel)
The collection bottle incorporates a reliable Overflow Protection Valve that closes off the tube to the vacuum in the event of a full bottle. In addition, the replaceable in-line filter protects the vacuum pump from contaminants and any moisture that comes through the bottle. Graduations on the collection bottle indicate the amount of fluid collected and when it's ready to be emptied. The Handheld Vacuum Pipette has an ergonomic design that's comfortable in the hand during prolonged use. Pressing the button applies the vacuum. The pipette accepts Pasteur pipettes, serological pipettes, and also comes with 2 accessory pipette cones. One cone is designed to accommodate 200/300ul pipette tips, the other has a thin, stainless steel capillary for fine control aspirating.
Specifications
| Max. Aspiration | 14 mL/sec |
| Max. Flow | 15 L/ min. |
| Vacuum Range | -30 to -750 mbar |
| Noise Level | 59.6 db |
| Vacuum Pump | oil free piston |
| Dimensions | 9.5 x 7.9 x 13.5 in. |
| Weight | 4.8 lbs |
| Warranty | 2 years |
| Item # | Description |
| V0020 | 115 V ASPIRE Aspirator |
| V0020-E | 230 V ASPIRE Aspirator |
| V0020-1SL | Extra Long Single Channel Stainless Steel Adapter |
| V1002-8 | Eight channel adapter for 200 µl pipette tips |
| V1002-S8 | Eight channel adapter, fine tip, stainless steel |
RephiQuik Syringe Filters
RephiQuik non-sterile syringe filters are a fast and efficient tool for analytical sample preparation.
As a result of high quality polypropylene (PP) housing and low extractable membranes, the filters deliver broad chemical compatibility and excellent performance consistency.
The non-sterile filters are available in 13 mm & 32 mm diameters and 5 types of membranes (PTFE, PVDF, PES, MCE and Nylon) for various application needs, such as HPLC/GC sample preparation or many other applications in pharmaceutical, environmental, biological, food, beverage and agricultural fields.
Please contact your trusted MIDSCI Lab Consultant for other available options on any discontinued items.
Argos Syringe Filters are ideal for filtering culture media and organic solvents in a wide range of applications. All items are tested for filter efficiency and housing integrity. Filter housing is manufactured and tested to withstand pressures of up to 75 PSI. Membrane materials include PES, PVDF, MCE, Nylon, and PTFE. Filters are individually wrapped and certified non-pyrogenic and RNase/DNase free.
Specifications:
- Available in 13 or 30 mm diameters
- Choice of .45 um or .22 um pore size
- Designed for low hold-up to minimize sample loss
- Lot numbers printed on sterile packs for easy traceability
- Packaged in convenient, peel-open package
- Certified non-pryogenic & gamma irradiated
(Please call for part numbers and pricing on filters w/ MCE, PTFE, or Nylon membranes)
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