ig 10B
Intact Genomics ig 10B chemically competent cells (E. coli) are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. This derivative of DH10B provides the highest efficiency in the industry.

• Most efficient chemically competent cells in the industry (up to 10X more efficient!)
• May easily be used in place of NEB 10-Beta, TOP10 from Invitrogen or other common brands
• Significantly more affordable

Specifications
Competent cell type:                Chemically competent
Derivative of:                            DH10B
Species:                                   E. coli
Format:                                    Tubes
Transformation efficiency:         â¥1.0 x 1010 cfu/µg pUC19 DNA
Blue/white screening:               Yes
Shipping condition:                   Dry ice

Quality Control
Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product. Each lot is repeatedly compared side-by-side with leading competitors to ensure our products outperform the competitor before product launching. 

Reagents Needed for One Reaction
ig 10B chemically competent cells:     50 µl
DNA (or pUC19 Control, 10 pg/µl):          1 µl
Recovery medium:                                  1 ml

Contents & Storage

1. ig 10B competent cells: -80 °C
2. pUC19 control DNA: -20 °C
3. Recovery medium: 4 °C

Genomic Features
ig 10B (DH10B derivative) chemically competent cells have the following features:

• 80lacZM15 marker provides α-complementation of the β-galactosidase gene with blue/white screening
• mcrA genotypic marker and the mcrBC, mrr deletion allows for cloning DNA that contains methylcytosine and methyladenine

Genotype
F â mcrA (mrr-hsdRMS-mcrBC) endA1 recA1 80dlacZM15 lacX74 araD139 (ara, leu)7697 galU galK rpsL (StrR) nupG -

General Guidelines
Follow these guidelines when using ig 10B chemically competent E. coli.

• Handle competent cells gently as they are highly sensitive to changes in temperature or mechanical lysis caused by pipetting.
• Thaw competent cells on ice, and transform cells immediately following thawing. After adding DNA, mix by tapping the tube gently. Do not mix cells by pipetting or vortexing.

Protocol

• Remove competent cells from the -80 °C freezer and thaw completely on wet ice (10-15 minutes).
• Aliquot 1-5 µl (1 pg-100 ng) of DNA to the chilled microcentrifuge tubes on ice.
• When the cells are thawed, add 50 µl of cells to each DNA tube on ice and mix gently by tapping 4-5 times. For the pUC19 control, add 1 µl of (10 pg/µl) DNA to a chilled microcentrifuge tube, prior to adding 50 µl of cells. Mix well by tapping.
• Incubate the cells with DNA on ice for 30 minutes.
• After 30 minute ice incubation, heat shock the cells at 42 °C for 45 seconds.
• Transfer the tubes to ice for 2 minutes.
• Add 950 µl of Recovery Medium or any other medium of choice to each tube.
• Incubate tubes at 37 °C for 1 hour at 210 rpm.
• Spread 50 µl to 200 µl from each transformation on Pre-warmed selection plates.
• Incubate the plates overnight at 37 °C.

VitroINK® Bioinks

Ready-to-use bioinks for 3D bioprinting

VitroINK® are ready-to-use, xeno-free (animal origin-free) bioink system either unmodified or modified with either RGD, Collagen-memetic, IKVAV, or YIGSR peptides or with Matrix Metalloproteinases (MMP) for biodegradable bioink.

VitroINK® is a family of the ready-to-use, xeno-free tunable bioink system that requires no UV,  no temperature/pH curing, or chemical cross-linking. The system is ready-to-use at room temperature, neutral in pH, transparent, and has excellent visibility after printing and cell culture. Due to the unique shear-thinning and rapid recovery mechanical property, VitroINK® can maintain the printed structure without UV or other special curing methods. Adding cell culture medium after printing can further stabilize the printed structure and support cell growth. Cells can be pre-mix with VitroINK® using our VitroINK® Mixing Kit for mixing ratios at 3:1 or 10:1. Different versions of VitroINK® may incorporate multiple biological functional ligands to promote cell attachment, cell-matrix interactions, cell proliferation, motility/migration and differentiation for many different applications.

Specifications

• Xeno-free tunable modified bioink.
• Ready-to-use at room temperature
• No UV, temperature/pH curing, or chemical cross-linking required
• Neutral pH
• Transparent. Excellent visibility after printing and cell culture
• Pre-mix with cells using the included VitroINK® Mixing Kit
• Ships room temperature. Store at 2-8°C
• Size: 3 mL

TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.   

TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.

To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.

For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

VitroINK® 10:1 Mixing Component Pack
 
Single-use components for 10:1 mixing ratio for VitroINK® Mixing Kit. DISPENSER NOT INCLUDED

The VitroINK® 10:1 Mixing Component Kit includes a 1 mL syringe, connector and mixing head to replace the one-time-use components in the full VitroINK® Mixing Kit – Complete Pack (Item # IMK00-1) for 10:1 VitroINK® and cells mixing.

This package is sterilized and disposable for one-time use.

Kit Contents and Specifications

• Disposable mixing kit components for VitroINK® kit:
• (1) 1 mL syringe
• (1) connector and tubing
• (1) mixing head
• Sterile
• Use for 10:1 mixing ratio
• Ships room temperature. Store at room temperature.

ReadyStrain™ II Sterile Cell Straining Kits

For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

VitroINK® 3:1 Mixing Component Pack

For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

VitroINK® Mixing Kit – Complete Pack
 
Mix VitroINK® bioink with cells at 3:1 and 10:1 ratios. Full mixing kit with Dispenser and single-use components.

Mixing cells with the bioink is a critical step for 3D bioprinting. Because of the differences in viscosity of the cell suspension and bioink, the traditional manual mixing methods create a lot of air bubbles and non-uniform mixture which leads to the unstable printed structure, difficulty for cell observation and affect cell viability.

The VitroINK® Mixing Kit was designed to provide a robust mixing of the bioink and cells. Cells can be prepared with VitroINK® for a 3:1 mixing ratio using the 3 mL syringe or a 10:1 mixing ratio using the 1 mL syringe. To prepare the bioprinter cartridge, the VitroINK® and cell suspension are placed in the mixer and is dispensed through the connector and mixing head. Wait 10-20 minutes for the mixture to stabilized and the cartridge is ready for printing. There is no UV, no temperature/pH curing, or chemical cross-linking for the VitroINK® system. Adding cell culture medium to cover the printed structure can further stabilize and support cell growth. The bioprinted cells are ready for incubation.

The syringe, connector and mixing head are disposable for one-time use. Order the sterilized replacement mixing kit components to ensure the robust results for every single mixing.

Kit Contents and Specifications

• Disposable mixing kit components for VitroINK bioink:
• (1) Dispenser
• (1) 1 mL syringe
• (1) 3 mL syringe
• (1) connector and tubing
• (1) mixing head
• Sterile
• Use for 3:1 or 10:1 mixing ratio
• Ships room temperature. Store at room temperature.

Harvest is the first product of its kind, providing all the necessary parts for filtration of cell suspensions and combining them into a single, preassembled, ready to use, sterile packet. The kits eliminate the contamination risks associated with opening multiple packages, and assembling separate pieces. The individual sterile packets mean researchers are no longer forced to expose entire bags of 50mL tubes and corresponding caps to perform one filtration. Instead they can open a single ReadyStrain packet directly under the hood.
  

• Kit Includes: strainer, 50 mL tube & cap
• Opens directly under the hood reducing risk of contamination
• Pre-assembled - ready to use
• Guaranteed sterile - gamma
• Packaged in easy-open "tear-strip" bags

For any singular Distributor customer order/sale price of over $5,000 there will be no return allowed. For any order of $0-$4,999 Distributor may cancel/return any or all Products with a 15% restocking fee within 30 days after delivery of products to the customer by Distributor. After 30 days from receipt of the product from the customer, there will be no return allowed.

VitroINK® – Bioink Starter Kits
 
VitroINK® (3 mL) + Full Mixing Kit with Dispenser

VitroINK® Bioink Starter Kit contains both bioink and the complete mixing kit to prepare the cell bioink mixture for 3D bioprinting. The kit includes choice of bioink + the mixing kit:

• VitroINK 3D (3 mL) + VitroINK® Mixing Kit with Dispenser
• VitroINK RGD (3 mL) + VitroINK® Mixing Kit with Dispenser
• VitroINK COL (3 mL) + VitroINK® Mixing Kit with Dispenser
• VitroINK MMP (3 mL) + VitroINK® Mixing Kit with Dispenser
• VitroINK IKVAV (3 mL) + VitroINK® Mixing Kit with Dispenser
• VitroINK YIGSR (3 mL) + VitroINK® Mixing Kit with Dispenser

The VitroINK® Mixing Kit includes a dispenser, 1 mL syringe, 3 mL syringe, connectors and mixing head to get you started.

VitroINK® is a ready-to-use tunable bioink modified with functional ligands, and cell adhesive peptide, promoting cell attachment and cell-matrix interactions. This RGD modified bioink can enhance cell adhesion, proliferation, motility/migration, and differentiation in different applications.

VitroINK® Mixing Kit was designed to provide a robust mixing of bioink and cells. Cells can be prepared with VitroINK® for a 3:1 mixing ratio using the 3 mL syringe or a 10:1 mixing ratio using the 1 mL syringe. Put the VitroINK® and cell suspension in the mixer and gently press the mixture through the connector and mixing head to your bioprinter cartridge. Wait 10-20 minutes for the mixture to stabilize before printing. There is no UV,  no temperature/pH curing, or chemical cross-linking for the VitroINK® system. Simply adding cell culture medium to cover the printed structure can further stabilize and support cell growth. The bioprinted cells are ready for incubation.

Contents and Specifications

VitroINK®

• Xeno-free tunable modified bioink.
• Ready-to-use at room temperature
• No UV, temperature/pH curing, or chemical cross-linking required
• Neutral pH
• Transparent. Excellent visibility after printing and cell culture
• Pre-mix with cells using the included VitroINK® Mixing Kit
• Ships room temperature. Store at 2-8°C
• Size: 3 mL

VitroINK® Mixing Kit

• Complete mixing kit.  Includes:
• (1) 1 mL syringe
• (1) 3 mL syringe
• (1) connector and tubing
• (1) mixing head
• Sterile
• Use for 3:1 or 10:1 mixing ratio
• Size: 3 mL

10 Layer BioFactory™ Culture Chambers with Tube 

Reporter gene based Luminescent assay systems from Perkin Elmer provide a homogeneous assay with 96, 384, and 1536 well formats, no DTT (no smell formulation), Ease of storage (2 to 8°C) and shipping (at RT). Different assay formats allow for varying levels of intensity and half-life depending on your application.

• Choose britelite plus:
• Half-life = 30 min
• Brightest signal = when looking for maximum sensitivity
• Shortest half-life = for continuous processing of samples

• Choose neolite:
• Half-life = 150 min
• High signal intensity, longer lasting signal = good balance
• For both continuous and batch processing of samples
• Best robustness (less dependent on mixing quality)
• Less sensitive to phenol red
• Can be stored 1 month at 22°C

• Choose steadylite plus:
  • Half-life = 240 min
  • Longest signal half-life = for batch processing of samples
  • Super-sensitivity not needed

5-Layer Polystyrene Cell Culture Flask

Reporter gene based Luminescent assay systems from Perkin Elmer provide a homogeneous assay with 96, 384, and 1536 well formats, no DTT (no smell formulation), Ease of storage (2 to 8°C) and shipping (at RT). Different assay formats allow for varying levels of intensity and half-life depending on your application.

• Choose britelite plus:
• Half-life = 30 min
• Brightest signal = when looking for maximum sensitivity
• Shortest half-life = for continuous processing of samples

• Choose neolite:
• Half-life = 150 min
• High signal intensity, longer lasting signal = good balance
• For both continuous and batch processing of samples
• Best robustness (less dependent on mixing quality)
• Less sensitive to phenol red
• Can be stored 1 month at 22°C

• Choose steadylite plus:
  • Half-life = 240 min
  • Longest signal half-life = for batch processing of samples
  • Super-sensitivity not needed