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PR1MA™ Signature Cell Strainers

Clean, reliable filtration with every use.

The PR1MA™ Cell Strainer system is a fast, efficient, and easy-to-use solution designed to streamline the process of cell isolation and filtration. Ideal for flow cytometry, this advanced straining system is perfect for detaching, isolating, and filtering cells from clusters and tissue samples.

Each cell strainer features a durable polypropylene frame supporting a nylon mesh, available in eight distinct, color-coded pore sizes ranging from 5 uM to
100 uM. Eight fine mesh options offer enhanced control for filtering cell aggregates and clumps, improving experimental precision.

The innovative frame design significantly improves filtration performance. By eliminating the “air-lock” effect, PR1MA™ Signature Cell Strainers promote faster flow-through and better aeration, reducing spillover and processing time.

Key Features

• Available in 8 color-coded pore sizes (5 uM to 100 uM) for precise cell sorting
• Fast flow-through thanks to advanced polypropylene frame design
• Includes universal adapter for tubes smaller than 50 mL
• Compatible with 6-well plates, flat-bottom plates, and culture dishes
• Interlocking and stackable design for improved lab workflow
• Built-in 1.5 mm feet for elevated straining into flat vessels
• Supplied sterile in individual blister packaging
• Certified RNase-, DNase-, pyrogen-, and cytotoxin-free

Specifications

Item #	Size	Color	Sterility	Description	Packaging
PR-CS5-1S	5 uM	Orange	Sterile	PP frame, nylon mesh	Indiv. Wrap blister pks, w/ 1 reducing adapter, 50/pk
PR-CS10-1S	10 uM	Teal	Sterile	PP frame, nylon mesh	Indiv. Wrap blister pks, w/ 1 reducing adapter, 50/pk
PR-CS20-1S	20 uM	Red	Sterile	PP frame, nylon mesh	Indiv. Wrap blister pks, w/ 1 reducing adapter, 50/pk
PR-CS30-1S	30 uM	Purple	Sterile	PP frame, nylon mesh	Indiv. Wrap blister pks, w/ 1 reducing adapter, 50/pk
PR-CS40-1S	40 uM	Blue	Sterile	PP frame, nylon mesh	Indiv. Wrap blister pks, w/ 1 reducing adapter, 50/pk
PR-CS50-1S	50 uM	Grey	Sterile	PP frame, nylon mesh	Indiv. Wrap blister pks, w/ 1 reducing adapter, 50/pk
PR-CS70-1S	70 uM	White	Sterile	PP frame, nylon mesh	Indiv. Wrap blister pks, w/ 1 reducing adapter, 50/pk
PR-CS100-1S	100 uM	Yellow	Sterile	PP frame, nylon mesh	Indiv. Wrap blister pks, w/ 1 reducing adapter, 50/pk
CELLADAPT	PR1MA™ Signature Reducing Adapter or 15 / 5 mL, 12 x 75 mm & 100 mm tubes, 25/pk

  

Chambered Cell Culture Slides

• Cells grow directly on the slide without any need for future transfer
• Ideal for growing and viewing cells under a microscope on a standard 25mm x 75mm slide
• Uniform hydrophylic surface is ideal for consistent cell attachment
• Slides are labeled with numeric markings
• Packing trays can be used as incubation racks in CO2 incubators
• Adhesive free

PR1MA™ Centrifuge Tube Filter Systems

• Eliminate a sample transfer step by filtering directly into a 50mL tube
• Polyethersulfone (PES) membrane for high flow and low binding
• One Centrifuge Tube Stand included with each case of 50mL Centrifuge Tube Filter Systems
• Centrifuge tube Stands are manufactured from polypropylene and are autoclavable, reusable and recyclable
• Centrifuge Tube Stand will hold one 50mL tube, three 15mL tubes and three microcentrifuge tubes (1.5mL)

Specifications
 

TPP Filtermax Filtration Units

The TPP Filtermax Filtration System is engineered for high-performance laboratory filtration with a premium 0.22 µm PES membrane. Its quadratic design offers a 20% larger filtration surface compared to circular membranes, improving throughput and saving valuable refrigerator space. The stable square bottom ensures secure handling during use, reducing the risk of tipping.

Each unit is designed for user comfort and flexibility. The tapered neck allows a firm grip, even with gloved hands, and the 45 mm neck thread ensures compatibility with standard bottles. Researchers can select a complete filter system, top only, or flask only to fit their workflow.

The Filtermax series is ideal for sterile filtration of media, buffers, and other laboratory liquids where speed, reliability, and sterility are essential.

Key Features

• Large square filter PES membrane (0.22 µm)
• High flow rate and low protein binding
• Square design for stability and optimized fridge storage
• Ergonomic tapered neck for slip-free grip
• 45 mm neck thread for bottle compatibility
• Flexible options: filter system, filter only, or flask only

Quality Assurance

• Gamma-sterilized, DNAse/RNAse-Free, and pyrogen-Free
• Individually wrapped in a peel-off plastic wrapper
• Screw cap & vacuum accessories included in each sterile package

Specifications
 

Contact us today to request a sample and experience the difference of TPP Filtermax in your lab.

TPP Clipmax Chamber Slide System

The TPP Clipmax Chamber Slide System (10 cm²) is a unique combination of a tissue culture flask and a removable microscope slide (25 x 75 mm), designed to simplify your cell culture and imaging workflows. Grow cells directly in the chamber, then remove the slide for easy fixation, staining, and imaging - without the need for transfer steps that increase contamination risk.

Clipmax is especially suited for cell culture, immunocytochemistry (ICC), immunofluorescence (IF), and cell analysis workflows, allowing you to work directly on the crystal-clear microscope slide for reliable, reproducible results.

Key Features

Optimal Optical Clarity

• Crystal-clear plastic microscope slide designed for cell imaging
• Suitable for staining, fixation, and especially for immunofluorescence measurements
• Solvent-resistant surface (acetone, ethanol, xylene)

Applications

• Cell culture chamber slide for imaging and analysis
• Immunocytochemistry (ICC) and immunofluorescence (IF) studies
• Staining and fixation directly on the microscope slide
• Ideal for researchers needing a media-saving, solvent-resistant, chambered slide system 

Streamline your imaging workflows with TPP Clipmax – culture, stain, and analyze cells
directly on one slide. Contact us today for a sample! 

 
PR1MA™ Cell Strainers

PR1MA™ cell strainers from MIDSCI™ can be used for many applications including isolating cells and removing clumps or debris. They are compatible with standard 50 mL centrifuge tubes. The exclusive Pestle design improves the manipulation of material in the strainer during operation.

• Polypropylene housing with nylon mesh filter eliminates the need to fabricate filters from gauze, paper, nylon mesh, or other materials
• The elongated lip on the housing extends beyond the edge of the centrifuge tube enabling aseptic handling with forceps
• Extended gripping tab on housing for easy handling
• Available in three porosities: 40, 70 & 100 µm
• Color-coded by mesh porosity for easy identification
• Packaged sterile (gamma irradiated) in individually wrapped peel-to-open paper/plastic wrapper or bulk packed in bags

Notice for U.S. Customers – RWD Consumables
Due to changes in U.S.–China tariffs, we will pause sales of these items after Tuesday, April 22, until further notice.
This is in anticipation of the expected De Minimis rule change on May 2.

For more information or alternative product recommendations, please contact us.

RWD Cell Separation Assembly Kit

Applications include immunology research, oncology research, neuroscience, stem cell research, and cell therapy.

RWD magnetic microbead cell separation products consist of Nano-Bead Cell Separation Platform and Nano-Beads (Column Based and Column free). Nano-sized magnetic beads do not need elution, and the separated cells can be directly used in downstream experiments such as flow cytometry, cell culture, single-cell sequencing, and more.

• High purity, high viability, and high recovery rate
• Obtained cells are compatible with downstream applications
• Mild, non-toxic, biodegradable

Kit Includes

• 1 box of LarSep Columns (25/pk)
• 1 LSC Separator (single channel)
• 1 Separation Stand

Work Flow

• Antibody incubation: Incubate the antibody with cell suspension at 4ºC for 10-15 mins.
• Microbead incubation: Incubate the magnetic microbeads with cell suspension at 4ºC for 10-15 mins.
• Magnetic separation: Put the separation column into the magnetic field and add the cell suspension into the column.
• Only cells labeled with magnetic beads will be captured in the column.
• Elution: Remove the column from the magnetic field and add a buffer to elute the positive cells.

Notice for U.S. Customers – RWD Consumables
Due to changes in U.S.–China tariffs, we will pause sales of these items after Tuesday, April 22, until further notice.
This is in anticipation of the expected De Minimis rule change on May 2.

For more information or alternative product recommendations, please contact us.

RWD Human Cell Separation Kits

CD3+ Cell Separation Kit - K1201-10

For positive selection or depletion of CD3+ T cells from peripheral blood, thymus, lymphoid, and tumor tissue.

CD4+ Cell Separation Kit - K1202-10

For positive selection or depletion of T helper cells (Th) from human peripheral blood, umbilical cord blood, lymph nodes, thymus, spleen, skin, and other samples.

CD8+ Cell Separation Kit - K1203-10

For positive selection or depletion of cytotoxic T cells (Tc) from human peripheral blood, umbilical cord blood, lymph nodes, thymus, spleen, skin, and other samples.

CD14+ Cell Separation Kit - K1204-10

For positive selection or depletion of monocytes from human peripheral blood, umbilical cord blood, spleen, lymph nodes, and other samples.

CD45+ Cell Separation Kit - K1205-10

For positive selection or depletion of white blood cells from human peripheral blood and lymphoid tissue samples.

CD19+ Cell Separation Kit - K1206-10

For positive selection or depletion of B cells from human peripheral blood, lymph nodes, bone marrow, and other single-cell suspensions.

Human NK Cell Isolation Kit - K1207-10

For negative selection or depletion of NK cells from single cell suspension of human peripheral blood. Non-NK cells, i.e., T cells, B cells, stem cells, dendritic cells, monocytes, granulocytes, and red blood cells are labeled by biotin antibodies coupled to magnetic beads. High purity NK cells are obtained by isolation of magnetic bead labeled cells.

*All kits are for research use only.

Specifications
 

Item #	Cell Type	Size	Capacity
K1201-10	T Cells	2 x 1 mL	For 1 x 10^9 Total Cells
K1202-10	T Helper Cells	2 x 1 mL
K1203-10	Cytotoxic T Cells	2 x 1 mL
K1204-10	Monocytes	2 x 1 mL
K1205-10	Leukocytes	2 x 1 mL
K1206-10	B Cells	2 x 1 mL
K1207-10	NK Cells	2 x 2 mL

 

Notice for U.S. Customers – RWD Consumables
Due to changes in U.S.–China tariffs, we will pause sales of these items after Tuesday, April 22, until further notice.
This is in anticipation of the expected De Minimis rule change on May 2.

For more information or alternative product recommendations, please contact us.

RWD High Activity Enzymatic Digestion Kits

Optimized enzymatic hydrolysis formulation for efficient and gentle digestion. Preserves the integrity of surface antigenic epitopes. Store in refrigerators at 4°C or -20°C.

• Targeted optimization of enzymatic hydrolysis formula can ensure high cell viability.
• Preserving cell surface epitopes leads to no effect on downstream experiments.

Notice for U.S. Customers – RWD Consumables
Due to changes in U.S.–China tariffs, we will pause sales of these items after Tuesday, April 22, until further notice.
This is in anticipation of the expected De Minimis rule change on May 2.

For more information or alternative product recommendations, please contact us.   

RWD Mouse and Microglia Cell Separation Kits

Mouse CD3+ Cell Separation Kit - K1301-10

For positive selection or depletion of mature T lymphocytes from mouse lymph nodes, thymus, tumors, and other samples.

Mouse CD4+ Cell Separation Kit - K1302-10

For positive selection or depletion of T helper cells (Th) from mouse lymph nodes, thymus, spleen, skin, and other samples.

Mouse CD8+ Cell Separation Kit - K1303-10

For positive selection or depletion of cytotoxic T cells (Tc) from mouse thymus, lymph nodes, spleen, and other samples.

Mouse CD45+ Cell Separation Kit - K1304-10

For positive selection or depletion of white blood cells from mouse lymphoid tissue or non-lymphoid tissue.

Mouse CD19+ Cell Separation Kit - K1305-10

For positive selection or depletion of B cells from mouse spleen and other samples.

CD11b+ (Microglia) cell separation Kit - K1306-10

For positive selection or depletion of microglia from human, adult mice, or rats brain tissue.

Specifications
 

Item #	Cell Type	Size	Capacity
K1301-10	T Lymphocytes	2 x 1 mL	For 1*10^9 Total Cells
K1302-10	T Helper Cells		For 1*10^9 Total Cells
K1303-10	Cytotoxic T Cells		For 1*10^9 Total Cells
K1304-10	Leukocytes		For 1*10^9 Total Cells
K1305-20	B Cells		For 2*10^9 Total Cells
K1306-10	Myeloid Cells		For 1*10^9 Total Cells

 

ig BL21 (DE3)
Intact Genomics chemically competent BL21(DE3) E. coli cells are suitable for transformation and routine protein expression.

Specifications
Competent cell type:                Chemically competent
Derivative of:                            BL21(DE3)
Species:                                   E. coli
Format:                                    Tubes
Transformation efficiency:         1.0 x 109 cfu/µg pUC19 DNA
Blue/white screening:               Yes
Shipping condition:                   Dry ice

Quality Control
Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product. Each lot is repeatedly compared side-by-side with leading competitors to ensure our products outperform the competitor before product launching. 

Reagents Needed for One Reaction
BL21(DE3) Chemically Competent Cells:             50 µl
DNA (or pUC19 Control, 10 pg/µl):                         1 µl
Recovery Medium:                                                 1 ml

Contents & Storage
BL21(DE3) Competent Cells:                -80 °C
pUC19 Control DNA:                            -20 °C
Recovery Medium:                                  4 °C

Genomic Features
BL21(DE3) chemically competent cells have the following features:

• Widely used host background
• T7 Expression Strain
• Deficient in both lon (1) and ompT proteases
• Resistant to phage T1 (fhuA2)
• B Strain

Genotype
F-ompT hsdS(rB- mB-) gal dcm (DE3)

General Guidelines
Follow these guidelines when using BL21(DE3) chemically competent E. coli.

• Handle competent cells gently as they are highly sensitive to changes in temperature or mechanical lysis caused by pipetting.
• Thaw competent cells on ice, and transform cells immediately following thawing. After adding DNA, mix by tapping the tube gently. Do not mix cells by pipetting or vortexing.

Transformation Protocol

• Remove competent cells from the -80 °C freezer and thaw completely on wet ice (10-15 minutes).
• Aliquot 1-5 µl (1 pg-100 ng) of DNA to the chilled microcentrifuge tubes on ice.
• When the cells are thawed, add 50 μl of cells to each DNA tube on ice and mix gently by tapping 4-5 times. For the pUC19 control, add 1 µl of (10 pg/µl)
• DNA to a chilled microcentrifuge tube, prior to adding 50 µl of cells. Mix well by tapping.
• Incubate the cells with DNA on ice for 30 minutes.
• After 30 minute ice incubation, heat shock the cells at 42 °C for 45 seconds.
• Transfer the tubes to ice for 2 minutes.
• Add 950 µl of Recovery Medium or any other medium of choice to each tube.
• Incubate tubes at 37 °C for 1 hour at 210 rpm.
• Spread 50 μl to 200 μl from each transformation on pre-warmed selection plates.
• Incubate the plates overnight at 37 °C. 

ig 5-Alpha
Intact Genomics 5-alpha chemically competent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. ig 5-alpha chemically competent cells are at least twice the transformation efficiency of the nearest competitor.

Number of Colonies per Transformation Test*

• Up to 2x higher efficiency than any competitor
• Exact same Genotype as NEB's 5-Alpha Cells

Specifications
Competent cell type:        Chemically competent
Derivative of:                    5-alpha
Species:                            E. coli
Format:                             Tubes
Transformation efficiency:      1 x 109 cfu/µg pUC19 DNA
Blue/white screening:           Yes
Shipping condition:               Dry ice

Quality Control
Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product. Each lot is repeatedly compared side-by-side with leading competitors to ensure our products outperform the competitor before product launching. 

Reagents Needed for One Reaction
ig 5-alpha chemically competent cells:  50 µl
DNA (or pUC19 Control, 10 pg/µl):           1 µl
Recovery medium:                                   1 ml

Contents & Storage
ig 5-alpha competent cells:   -80 °C
pUC19 control DNA:                 -20 °C
Recovery medium:                       4 °C

Genotype
80 (lacZ)M15 fhuA2 (argF-lacZ)U169 phoA glnV44 gyrA96 recA1 relA1 endA1 thi-1hsdR17

General Guidelines
Follow these guidelines when using ig 5-alpha chemically competent cells.

• Handle competent cells gently as they are highly sensitive to changes in temperature or mechanical lysis caused by pipetting.
• Thaw competent cells on ice, and transform cells immediately following thawing. After adding DNA, mix by tapping the tube gently. Do not mix cells by pipetting or vortexing.

Protocol

• Remove competent cells from the -80 °C freezer and thaw completely on wet ice (10-15 minutes).
• Aliquot 1-5 µl (1 pg-100 ng) of DNA to the chilled microcentrifuge tubes on ice.
• When the cells are thawed, add 50 μl of cells to each DNA tube on ice and mix gently by tapping 4-5 times. For the pUC19 control, add 1 µl of (10 pg/µl) DNA to a chilled microcentrifuge tube, prior to adding 50 µl of cells. Mix well by tapping. 4) Incubate the cells with DNA on ice for 30 minutes.
• After 30 minute ice incubation, heat shock the cells at 42 °C for 45 seconds.
• Transfer the tubes to ice for 2 minutes.
• Add 950 µl of Recovery Medium or any other medium of choice to each tube.
• Incubate tubes at 37 °C for 1 hour at 210 rpm.
• Spread 50 μl to 200 μl from each transformation on Pre-warmed selection plates.
• Incubate the plates overnight at 37 °C.