Resources

Lab Product Links & Literature

Higher DNA yield with Buccal Swabs

Improve DNA collection, storage and purity with Isohelix Buccal Swabs and kits

Improve DNA collection, storage and purity with Isohelix Buccal Swabs and kits

                Buccal swabs are used across a wide variety of fields.  They are used in research laboratories, forensic labs, genetic testing laboratories, veterinary genotyping and diagnostics laboratories, paternity testing laboratories, and clinical diagnostics, to name a few.  The type of buccal swab used can not only have an effect on the amount of DNA collected, but can also impact further stability and extraction in downstream applications.  Isohelix swabs have a novel matrix that ensures efficient collection and increases DNA yield and ease of extraction.  When paired with Isohelix kits, even higher yields and purity can be obtained.

Isohelix provides an integrated solution for DNA processing with products for sampling, transporting, stabilizing, and extraction while maintaining high yields and purity.  Isohelix swabs provide significant advantages over other commercially available swabs. (Figure below)

http://2.bp.blogspot.com/-K8bxAmESZV4/UvOzHoNzKZI/AAAAAAAAAVA/PF81tDr4Y_U/s1600/isohelix+blog+post+1.png

 

What sets Isohelix apart?

       High yielding swabs for buccal cell DNA collection

       Unique swab matrix greatly improves DNA yields

       Alternative to blood collection

       Suitable for human and veterinary use

There are various different swab types available to fit your needs.  All swabs are ethylene oxide treated and are tested for DNA contamination.  The SK-1 swabs come with a 5 mL tube. After collecting the shaft can be easily snapped above swab head.  The SK-1 swabs are best paired with Dri-capsules, which allow for storage of the DNA for at least 3.5 years at room temperature!

http://2.bp.blogspot.com/-SThPdnP7uhQ/UvOzWR5-maI/AAAAAAAAAVI/ACSl4nx6M60/s1600/sgc-50.jpg



The swabs can also be purchased with a 2 mL tube.  After collection, the lid on the 2 mL tube is securely closed.  If air venting is desired for drying of the swab in the field, the stopper can be opened while still having a closed cap.  The swab can then be removed by pulling on the shaft. 

http://4.bp.blogspot.com/-yHRwI7afpIM/UvOzsB42M5I/AAAAAAAAAVQ/PbGzG8w0rVs/s1600/isohelix+blog+post+3.png

 

If desired, swabs can also be purchased individually wrapped, two per pack, or three per pack. 

 

Need to effectively stabilize and/or isolate your DNA?

The DDK kit is a no column quick processing kit.  The DDK kit includes a stabilization reagent (stabilizes for up to 5 years) and isolation reagent that maintains both high yield and purity.  Up to 10 µg of DNA can be obtained from each swab with the DDK kit

The two cartridge based kits, XtraClean and Xtreme are important when extra high purity is desirable.  These kits are great when downstream analysis assays are arrays or quantitative PCR. 


Need your DNA Rapidly?

Try the BEK Buccalyse
DNA Release Kit.  Obtain a 2 to 4µg yield in minutes! 

http://4.bp.blogspot.com/-8-aznbiUGsk/UvOz8xPDIKI/AAAAAAAAAVY/BaHe-80IG-4/s1600/isohelix+blog+post+4.jpg

Isohelix swabs and kits  can increase not only your DNA yield, but stability, purity, and ease of extraction.  Contact MIDSCI today for more information.   


Contact Us