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TheWell Bioscience VitroGel® STEM

Item #: ASVITROSTEM 

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VitroGel® STEM
 

ready-to-use, xeno-free (animal origin-free) hydrogel system for hPSCs 3D static suspension culture and scale-up

  • Establish stem cell spheroids directly from liquid nitrogen (5 min protocol)
  • High-quality 3D stem cells for differentiation and organoid formation
  • Easy scale-up (from laboratory scale to industrial scale)
  • Efficient cell harvesting or subculturing

VitroGel® STEM is a xeno-free (animal origin-free) hydrogel system developed to improve the performance of three-dimensional (3D) static suspension cultures and scale-up of human pluripotent stem cells (hPSCs) to create a high-throughput system to model various tissue and disease states.

This hydrogel system is ready to use with an optimized formulation that fully supports the rapid expansion of high-quality 3D stem cell spheroids with pluripotent properties. hPSCs directly thawed from liquid nitrogen or passaged from 2D matrix coated culture vessels can be immediately mixed with the hydrogel solution for static suspension cultures. Moreover, the optimization protocol is ideal for time-sensitive experiments, as it does not require excessive medium exchanges, which can ultimately save on time and materials. This hydrogel system is compatible with most hPSC culture media and tissue culture vessels. Due to the unique static suspension culture procedure, the requirement for microcarriers for large-scale bioreactors is eliminated, making cell harvesting simple and effective. The 3D stem cell spheroids that are developed using this system can be used for further sub-cultures, patterned differentiations, organoid development, or re-establishing 2D culture morphologies.

“Just add cells” 5 min protocol. No matrix coating required.

VitroGel® STEM is ready-to-use. Just mix with your hPSCs. There is no laborious matrix coating required to maintain and expand your stem cells.

 

Specifications

Formulation

Xeno-free, functional hydrogel

Use

3D static suspension culture for hPSCs

Operation

Ready-to-use at room temperature

Biocompatibility

Biocompatible, safe for animal studies

Injection

Injectable hydrogel for in vivo studies and lab automation

Cell Harvesting

20 min cell recovery using VitroGel® Cell Recovery Solution

pH

Neutral

Storage

Store at 2-8°C. Ships at ambient temperature

Sizes

10 mL and 2 mL

Number of Uses

(10 mL) 90-180 mL suspension culture

(2 mL) 15-30 mL suspension culture



TheWell products are never to be shipped to residential addresses. This decision has been made to safeguard the improper use and application of these products, particularly in the case of injectables and cell therapy treatments. TheWell has the right to refuse any orders that deem unfit use of their products or violate TheWell’s Terms of Use.   

 

TheWell products are sold for Laboratory Research Use Only, Not For Diagnostic or Therapeutic use, and are not to be administered to humans.

To ensure a seamless shopping experience, please provide a non-residential shipping address during checkout where your order can be safely received and stored.

  

SKU Number Description Volume Price Quantity Add to Cart
VHM02S VitroGel STEM 2 mL
132.00
Each
VHM02 VitroGel STEM 10 mL
429.00
Each
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TheWell Bioscience VitroGel® STEM
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