IBI High-Speed Plasmid Mini Kits

Fast and Economical Plasmid Minipreps

• Ideal for purification from bacterial culture
• Up to 30ug binding capacity
• Expected yield: 20-30ug for high-copy plasmid / 3-10ug for low-copy plasmid
• Perfect for cleanup prior to sequencing and cloning

The IBI High-Speed Plasmid Mini Kits are designed for the rapid isolation of plasmid or cosmid DNA from 1-4ml of bacterial cultures. The modified alkaline lysis method and RNAse treatment are used to create cleared cell lysate with minimal genomic DNA and RNA contaminants. In the presence of chaotropic salt, the plasmid DNA in the lysate binds to the glass fiber matrix in the spin column. The purified plasmid DNA is eluted by a low salt elution buffer or water. This procedure does not require DNA phenol-extraction or alcohol precipitation.

Sample:	1-4ml bacterial culture
Format:	Spin columns
Operation:	Centrifuge/Vacuum Manifold
Binding Capacity:	up to 30µg
Expectant Yield:	20-30µg for high-copy plasmid 3-10µg for low-copy plasmid
Operational Time:	30 min.
Applications:	PCR, Fluorescent or Radioactive Sequencing, Restriction Digests, DNA Labeling, Ligations