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These optically clear polyester films are ideally suited for Real Time PCR (qPCR). They feature an inert, ultra-clear, and strong encapsulated silicone adhesive that provides extraordinary performance in real-time PCR applications.
Encapsulated silicone greatly reduces the risk of sample contamination due to exposure to adhesive. Once the film is pressed onto the plate, the raised portion of the well penetrates the encapsulation and a tight seal is formed on just that surface (not over the sample). This silicone seal adheres aggressively and diminishes the possibility of "Edge Effect".
The plasticized backing of this film is scored on both ends to allow for two handed (precise) placement on the plate. Both film and adhesive maintain incredible clarity and are the choice for highly demanding manual and automated processes.
Certified free of RNase, DNase, Pyrogen, and nucleic-acid contamination.
Length |
141.27 mm |
Length (w/ end tabs removed) |
117.5 mm |
Width |
77.8 mm |
Overall Thickness |
2.8 mil |
Adhesive Thickness |
2.0 mil |
Material |
Polyester |
Adhesive |
Encapsulated Silicone |
Max. Temperature |
120 °C |
Min. Temperature |
-40 °C |
Primary Usages |
Real Time PCR (qPCR), PCR |
TiterSeal™ Sealing Film
- Eliminates the “edge effect” which can occur with plate lids (e.g. greater evaporation in the outside plate wells)
- Provides a secure seal and is easy to remove
- Rigid polyester film features good clarity and easy handling
- Can be used as a temporary economical seal while awaiting further processing
- Features two end-tabs with perforations
- General laboratory use
- Short-term storage
- Incubation
- ELISA
- -40°C to +120°C
ThermalSeal® RTS™ Optically Clear, Pressure Activated, Non-tacky Silicone Adhesive
The silicone adhesive is non-tacky until pressure is applied to the sealing surface which then creates a secure bond around the wells and perimeter of the plate. Also ideal for dry samples (e.g. soil, seeds, insects, etc.) because the non-activated adhesive above the wells remains non-tacky. A roller or sealing paddle is highly recommended for use with this product to ensure consistent and adequate pressure is applied during the sealing process.
- Provides an extremely strong seal to minimize evaporation
- Certified DNase- & RNase- free
- Chemically resistant to DMSO, acetonitrile, and methanol
- Ideal for use with flat-top, raised-rim and non-skirted PCR plates
- High optical clarity and low auto-fluorescence
- Available in roll format for automation: ThermalSeal® RTS™ Roll-Seal
- A complimentary sealing paddle is included in each box
Applications
- qPCR
- Long-term storage
- Protein crystallography
- HPLC
- -80°C to +105°C
Helpful Tips
- Sufficient pressure must be applied to activate the silicone adhesive
- Multiple swipes of the sealing paddle in both directions is highly recommended
- A 10 minute or greater wait period before thermal cycling increases the film-to-plate bond
Click to shop new and improved eXTReme™ 2 Seal Extra Strong Sealing Film
eXTReme™ Seal Sealing Film with Extra Strong Adhesive Minimizing Evaporation Without Residue
eXTReme™ Seal features an extra-strong thick adhesive to minimize evaporation, yet removes easily without leaving residue on the plate.
Features chamfered corners to eliminate plate overhang for robotics applications and tight storage conditions.
- Certified DNase- & RNase- free
- Ideal for use with raised-rim and non-skirted PCR plates
- Available in a larger cut for flat-top semi-skirted and full-skirted PCR plates: eXTReme™ Seal, Large
Application
- PCR
- Robotics
- -20°C to +120°C
Helpful Tips
- Selection of the proper film size will minimize evaporation
- The film should not overhang or ride up the wall of a raised-rim plate
- More complete coverage of skirted flat-top plates will reduce evaporation in the outer wells
- End-tab removal before thermal cycling is highly recommended
SealPlate® Sealing Film - The Industry Standard for Sample Protection
SealPlate® minimizes evaporation, prevents well-to-well contamination and spillage. Eliminates the “edge effect” which can occur with plate lids (e.g. greater evaporation in the outside plate wells).
- Provides a secure seal and is easy to remove
- Rigid polyester film features good clarity and easy handling
- Can be used as a temporary economical seal while awaiting further processing
- Features two end-tabs with perforations
Applications
- General laboratory use
- Short-term storage
- Incubation
- ELISA
- -40°C to +120°C
SealPlate® RollMate™ Pre-scored Film in a Convenient, Easy to Use Roll Format
Pre-scored film on a roll is a more economical and efficient alternative to rapidly seal microplates or racked test tubes; faster than applying single sheeted film. Self-standing, non-slip supports hold the pre-scored film roll securely in place for quick and easy bench-top dispensement. The roll unwind tension can be easily adjusted to user preference. Continuous backing liner can be torn off or fed directly into a waste basket for disposal.
- Economical
- Quicker, more efficient sealing
- Provides a secure seal and is easy to remove
- Rigid polyester film features good clarity and easy handling
- Can be used as a temporary seal while awaiting further processing
- General laboratory use
- Short-term storage
- Incubation
- ELISA
- -40°C to +120°C
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