PR1MA™ High Fidelity Hot Start Master Mix

• Leaves an A-overhang for TA cloning
• Ideal for difficult, high GC content sequences
• 10x fidelity of native Taq
• Ideal for long PCR, up to 10kb targets

PR1MA™ High Fidelity Hot Start Mix is a hot start 2x formulation which provides excellent sensitivity in low-copy number assays with 10x higher fidelity than Taq polymerase. The 2x master mix contains proprietary enhancers, an antibody-mediated hot-start mechanism, and a proofreading component for trouble-free PCR reaction assembly and performance.

The pre-optimized Hot Start Master Mix is supplied in a single tube, reducing the number of pipetting steps while improving throughput and reproducibility. The highly efficient buffer formulation and hot-start blend provide the ideal conditions for high-performance PCR and inactivity at room temperature thereby eliminating non-specific amplification.

PR1MA High Fidelity Master Mix

• Leaves a blunt end
• Rapid extension: up to 1 kb per 15 seconds
• 100x fidelity of native Taq
• Ideal for shorter, less complex targets

A 2x formulation which provides extreme sensitivity in low copy number assays with 100x the fidelity of native Taq, PR1MA High Fidelity Master Mix is perfect for shorter, less complex targets. The 2x master-mix contains proprietary enhancers and a proof-reading component for trouble-free PCR reaction assembly and performance. High Fidelity Master Mix delivers a unique balance of PCR sensitivity, high fidelity, versatility, and tolerance to inhibitors.

The pre-optimized Master Mix is supplied in a single tube, reducing the number of pipetting steps while improving throughput and reproducibility. The highly efficient buffer formulation provides the ideal conditions for high-performance PCR.

PR1MA™ qMAX One-Step RT-qPCR Kits

• RNA to cDNA to qPCR in one tube
• High purity enzyme formulation for enhanced stability and performance
• PR1MA Hot-Start Taq allows for preparation at room-temperature
• Compatible with all qPCR instruments
• Available for green fluorescence or probe detection

Both One-Step Kits are compatible with standard and fast cycling protocols and provide increased sensitivity, speed and reproducibility for a broad range of samples and targets.  The polymerase mix is available with different levels of ROX reference dye for compatibility with all qPCR instruments.

Two versions of our One-Step qPCR Kits are available:

qMAX™ Green One-Step Kits incorporate our proprietary intercalating dye which exhibits higher fluorescent and lower PCR inhibition than other popular dyes such as SYBR.

qMAX™ Probe One-Step Kits are optimized for use with popular TaqMan, Scorpions, and molecular beacon probes.

*Please note these products ship on dry ice.  Appropriate shipping charges apply unless otherwise noted on a quote.

PR1MA™ Mammalian Genotyping Kit

• DNA extraction and amplification in 1 hour
• Proprietary lysis buffer optimized for ear punches, tail snips and other mammalian tissues
• Single tube - no organic solvents or clean up procedures
• Enhanced PR1MA Hot Start Polymerase included

Traditionally, mammalian genotyping protocols have involved Proteinase K digestion, neutralization, organic extraction and lots of hands on time to get PCR-ready DNA.

The PR1MA Mammalian Genotyping Kit is quick and easy to use - add sample to the proprietary lysis buffer and incubate for 5-10 minutes, then add the deactivation buffer and incubate for 10 minutes. The crude lysate can then be amplified using fast PCR PR1MA Hot Start Taq Master Mix with red loading dye (included). The kit contains everything needed - just add sample and primers.

 
 
PR1MA 1 Hour Mammalian Genotyping Kit 

 
 
 PR1MA Genotyping Hot Start Master Mix, 2X Concentration with Red Dye

• RNA to cDNA to qPCR, in one tube
• High purity enzyme formulation for enhanced stability and performance
• Accuris Hot-Start Taq allows for preparation at room-temperature
• Blue dye facilitates pipetting and visualization in plates
• Available for green fluorescence or probe detection
• Multiplex formulation available for multiple target amplification

• 302nm midrange UV wavelength (optimal for EtBr excitation)
• Dual intensity settings (50% or 100%)
• Hinged UV Safety cover
• Use with SmartDocTM enclosure (E5000-SD) for gel imaging with a smart phone
• Compatible DNA stains: EtBr, SmartGlow™ LD and PS stain, Gel Red, Gel Green, SYBR® Green, SYBR® safe, and Diamond™ Nucleic acid Dye
• 2-yr warranty

This new UV transilluminator features a space-saving design while having 16 x 19 cm UV viewing surface to accommodate large gels or multiple smaller gels. The illuminated surface is comprised of a special black glass which is easy to clean, durable and scratch resistant.

Furthermore, an array of high intensity UV bulbs coupled with an optimized filter provides even illumination across the viewing surface and allows detection of small sample quantities. The dual intensity switch can be used to lower the UV intensity to 50% to reduce DNA damage during long exposure times, or switch to high intensity (100%) for viewing weak fluorescent signals or for photo documentation.

The acrylic UV blocking cover allows safe viewing of samples and has two friction hinges on the front edge to allow angling of the cover for gel access. The cover can be positioned to 90° (straight up) to fit a gel imaging hood, such as the SmartDoc™ imaging enclosure (E5000-SD).

Accuris MyVolt™ Mini Power Supply

• Compact and simple
• Choose constant 100 V or 200 V output
• Two pairs of output terminals

The Accuris MyVolt Mini is a compact electrophoresis power supply ideal for basic, constant voltage applications. Users can select constant 100 V or 200 V output, and the current can reach a maximum of 200 mA.

Two sets of output jacks are available to connect up to 2 gel rigs and these small power supplies can securely be stacked for use with additional gel rigs.

Specifications

Accuris SmartReader™ UV-Vis Microplate Reader
 
The Accuris SmartReader™ UV-Vis Microplate Reader (model MR9610) has been designed with ease-of-use and fast results in mind. This instrument brings extensive capabilities to the lab bench and is designed for the busy lab with multiple users. The large touch screen command center is extremely user-friendly and allows for fast set up of new protocols, as well as modifying and running existing protocols.

• Intuitive, 10-inch color touch screen control panel
• Accepts both 96 & 384-well plates
• Monochromator-based system
  • No filters required
  • Wavelength range from 200 to 1000 nm
• Stand-alone design
  • No PC or special software required
  • USB port for flash drive data transfer

• Inert yellow dye helps reduce pipetting errors
• Room temperature stable for up to 30 days
• Compatible with fast cycling protocols
• Highly sensitive for low copy number templates
• Includes PR1MA Hot Start Taq Polymerase

Successful PCR requires careful control of many variables.  Pipetting errors, poor performing polymerases, dNTP concentrations, are just a few of the variables that can all contribute to reaction problems.  PR1MA has developed their new qMAX™ Gold to control these variables and help you achieve the best possible amplification performance. To reduce the chance of pipetting errors, qMAX™ Gold includes an inert yellow dye so small volumes are easy to visualize in PCR plates.  

qMAX™ Gold is a ready to use, 2X mastermix of PR1MA Hot Start Taq enzyme, dNTPs, a sensitive fluorescent intercalating dye, and optimized reaction buffer.  The Hot Start Taq allows reaction set up at room temperature while the temperature stable formulation guarantees optimal performance even if the mix is left at room temperature for extended periods.

Just add primers and DNA targets to the mix, and then proceed to amplification.  qMAX™ Gold is compatible with all real time thermal cyclers and exhibits high sensitivity with normal or fast 2-step cycling protocols.
 *Please note these products ship on dry ice.  Appropriate shipping charges apply unless otherwise noted on a quote.
 
 

Specifications