PR1MA™ SmartCheck DNA Ladders

• Ready-to-use formulation includes loading buffer and tracking dye
• 500 µL suitable for 100 lanes (5 µL per lane)
• Higher intensity reference bands
• Ultra pure production allows economical ambient shipping

Technical Details
 

Protocol: Briefly vortex the tube and use 5 µL per lane. Additional loading buffer is not required.

Concentration: 0.1 mg/mL

Tracking dye: bromophenol blue and xylene cyanol

Buffer formulation: 10mM Tris-HCl (pH 8.0), 5mM EDTA, 12.5% glycerol, 0.008% bromophenol blue, 0.008% xylene cyanol

Shipping and Storage: SmartCheck™ DNA Ladders are shipped at ambient temperature. On arrival, store at -20°C for optimum stability and long-term storage up to 15 months.

Production: SmartCheck™ ladders are produced from proprietary plasmids, digested to completion. A multistep chromatography method is used to ensure purity and DNA quality.

Quality Control: Each lot of SmartCheck™ DNA Ladders is tested with a spectrophotometer to confirm DNA concentration, as well as agarose electrophoresis to confirm band sharpness.

Item	Description	Volume
PR4005-100	PR1MA™ SmartCheck™ 50bp DNA Ladder	500 µL / 100 Lanes
PR4005-500	PR1MA™ SmartCheck™ 50bp DNA Ladder	5 x 500 µL / 500 Lanes
PR4010-100	PR1MA™ SmartCheck™ 100bp DNA Ladder	500 µL / 100 Lanes
PR4010-500	PR1MA™ SmartCheck™ 100bp DNA Ladder	5 x 500µL / 500 Lanes
PR4100-100	PR1MA™ SmartCheck™ 1kb DNA Ladder	500 µL / 100 Lanes
PR4100-500	PR1MA™ SmartCheck™ 1kb DNA Ladder	5 x 500 µL / 500 Lanes

  

PR1MA™ Mammalian Genotyping Kit

• DNA extraction and amplification in 1 hour
• Proprietary lysis buffer optimized for ear punches, tail snips and other mammalian tissues
• Single tube - no organic solvents or clean up procedures
• Enhanced PR1MA Hot Start Polymerase included

Traditionally, mammalian genotyping protocols have involved Proteinase K digestion, neutralization, organic extraction and lots of hands on time to get PCR-ready DNA.

The PR1MA Mammalian Genotyping Kit is quick and easy to use - add sample to the proprietary lysis buffer and incubate for 5-10 minutes, then add the deactivation buffer and incubate for 10 minutes. The crude lysate can then be amplified using fast PCR PR1MA Hot Start Taq Master Mix with red loading dye (included). The kit contains everything needed - just add sample and primers.

 
 
PR1MA 1 Hour Mammalian Genotyping Kit 

 
 
 PR1MA Genotyping Hot Start Master Mix, 2X Concentration with Red Dye

PR1MA PCR Tool
 
Tool for opening and/or closing PCR strips or single tubes.  Compatible with flat or domed caps.  Can also be used for sealing film.

• Dimensional uniformity for superior thermal transfer
• AVST-01 flat caps are optically clear and ideal for qPCR
• Certified DNAse/RNAse, nucleic acid, PCR-inhibitor, and pyrogen-FREE

Please give us a call for a sample!

Pryme Ergonomic PCR 8-Strip Tubes

• Tube Characteristics
  • 0.1mL, low profile
  • Easy open - easy close
• Extreme Uniformity (EU) Tube Design
  • Uniform wall thickness ensures consistent even heating
  • Thin wall-bottomed tube for rapid heat transfer
• Pairs with EU âSealing Ring Band❠designed caps
  • Caps are significantly easier to apply and remove -- Repetitive Strain Injury (RSI) Friendly.
  • Significant reduction in evaporation due to a wider sealing area.
• Certified DNA, DNase, RNA, RNase and pyrogen-free
Please give us a call for a sample.

Order #	Description
B79601	Pryme PCR Ergonomic 8-Strip Tubes, 0.1mL, Low Profile, Natural, 120/cs
B79609	Pryme PCR Ergonomic 8-Strip Tubes, 0.1mL, Low Profile, White, 120/cs
B57801	Pryme PCR Ergonomic 8-Strip, Optical Flat (V2) Caps, Natural, 120/cs
B79701	Pryme PCR Ergonomic 8-Strip, Optical Flat (V1) Caps, Natural, 120/cs
C79701	Pryme PCR Ergonomic 8-Strip, Semi-Domed (V1) Caps, Natural, 120/cs

Thinwalled 0.2ml flat-top tubes with integral cap are suitable for use in all thermal cyclers with a 0.2ml block format.

Pryme PCR Standard Individual Tubes 0.2 & 0.5 mL

Thinwall flattop or domed capped with attached tubes are available in 0.2 mL and 0.5 mL volumes.   0.2 mL tubes are suitable for use in all thermal cyclers with standard 0.2 mL block formats.  0.5 mL tubes are suitable for use in all thermal cyclers with standard 0.5 mL block formats.  Available in clear tubes, individual colors, and assorted colors.  The thin wall feature accelerates heat transfer, providing shorter cycle times.

PR1MA™ Protein Markers

PR1MA™ All Blue, Regular Range Protein Markers

PR1MA™ All Blue, Regular Range Protein Markers are blue protein standards with 10 or 11 pre-stained proteins covering a wide range of molecular weights from 10 to 180 kDa in Tris-Glycine buffer (9 to 170 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore, and two reference bands (at 25 kDa and 72 kDa, in the presence of Tris- Glycine buffer) are enhanced in intensity.

Loading Volume

• 3 μL or 5 μL per loading for clear visualization during electrophoresis on a 15-well or 10-well mini-gel
• 1.5~2.5 μL per well for general Western blotting transfer

The markers are supplied at a concentration of approximately 0.1~0.5 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C, 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol)

PR1MA™ Tricolor Protein Markers

PR1MA™ Tricolor Protein Markers are three-color protein standards with 10, 12, or 13 pre-stained proteins covering a wide range of molecular weights from 5 to 245 kDa in Tris-Glycine buffer (3.5 kDa to 235 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for two reference bands, one green and one red band (at 25 kDa and 75 kDa, respectively, in the presence of Tris-Glycine buffer).

Loading Volume

• 3 μL or 5 μL per loading for clear visualization during electrophoresis on a 15-well or 10-well mini-gel.
• 1.5~2.5 μL per well for general Western blotting transfer.

The markers are supplied at a concentration of approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).

 
Specifications

Item#	Color	Bands	Range
PR5010-BL	Blue	10	9-180 kDA
PR5011-BL	Blue	11	9-180 kDA
PR5010-TC	Tri-Color	10	9-180 kDA
PR5012-TC	Tri-Color	12	9-245 kDA
PR5013-TC	Tri-Color	13	9-245 kDA

 

• Inert yellow dye helps reduce pipetting errors
• Room temperature stable for up to 30 days
• Compatible with fast cycling protocols
• Highly sensitive for low copy number templates
• Includes PR1MA Hot Start Taq Polymerase

Successful PCR requires careful control of many variables.  Pipetting errors, poor performing polymerases, dNTP concentrations, are just a few of the variables that can all contribute to reaction problems.  PR1MA has developed their new qMAX™ Gold to control these variables and help you achieve the best possible amplification performance. To reduce the chance of pipetting errors, qMAX™ Gold includes an inert yellow dye so small volumes are easy to visualize in PCR plates.  

qMAX™ Gold is a ready to use, 2X mastermix of PR1MA Hot Start Taq enzyme, dNTPs, a sensitive fluorescent intercalating dye, and optimized reaction buffer.  The Hot Start Taq allows reaction set up at room temperature while the temperature stable formulation guarantees optimal performance even if the mix is left at room temperature for extended periods.

Just add primers and DNA targets to the mix, and then proceed to amplification.  qMAX™ Gold is compatible with all real time thermal cyclers and exhibits high sensitivity with normal or fast 2-step cycling protocols.
 *Please note these products ship on dry ice.  Appropriate shipping charges apply unless otherwise noted on a quote.