PR1MA™ Luria Broth 1 Liter Buffered Capsules [Miller's LB Broth]

Each capsules has 25 g.  The 500 g package has 20 capsules, the 1 KG is 40 capsules and the 5 KG is 200 capsules.
 

CAS Number: 91079-40-2; 8013-01-2; 7647-14-5; 77-86-1; 1185-53-1
Storage Temperature: Room Temperature
Solubility: Water
 

RPI Media is now available in convenient pre-measured and pre-mixed capsules. Pre-buffered media eliminates messy weighing and pH adjustments.

Simply dissolve 1 capsule per 1 Liter of distilled water. Water must be 37°C or warmer to completely dissolve gelatin capsule. Agitate. Sterilize by autoclaving for 15 minutes before use. Buffer is set for 7.2 at 20°C using a 10mM solution of Tris/Tris HCl. pH will vary depending on temperature.


For maintenance and propagation of E. Coli. High Salt Formula. Molecular Biology Grade.


Ingredients:

• Tryptone: 10 g/L
• Sodium Chloride: 10 g/L
• Yeast Extract: 5 g/L
• Tris/Tris HCl: 1.5 g/L

Research or further manufacturing use only, not for food or drug use.

• Appearance: Capsules (cream to pale beige free flowing powder)
• pH (2.5 % Solution @25 ^oC): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Fair to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

PR1MA™ Agar, High Gel Strength, Powder

CAS Number: 9002-18-0
Solubility: Water
Storage Temperature: Room Temperature

Purified high gel strength agar with excellent properties for tissue culture and microbiological work.


Molecular Biology Grade


Research or further manufacturing use only, not for food or drug use.

• Appearance: White to Cream Powder
• Ash: ≤ 4.5 %
• Gel Point (1.5% Solution): 33 - 38 °C
• Gel Strength (1.5% Gel): ≥ 800 g/cm²
• Loss on Drying: ≤ 12.0 %
• Melting Point (1.5 %): 88.0 - 96.0
• pH (1.5% solution): 6.0 - 8.0

PR1MA™ Terrific Broth, Modified, Granulated

CAS Number: 91079-40-2; 8013-01-2; 7778-77-0; 7758-11-4
Solubility: Water
Storage Temperature: Room Temperature

Enriched media for maintenance and propagation of E. Coli and plasmid growth. Produces higher cell densities resulting in greater yields of DNA or recombinant problem. Glycerol is used as a carbon source with this formulation instead of glucose as it will not ferment to acetic acid.


Ingredients:

• Casein Digest Peptone: 12g/L
• Yeast Extract: 24g/L
• Dipotassium phosphate: 9.5g/L
• Monopotassium phosphate: 2.2g/L

Research or further manufacturing use only, not for food or drug use.

• DCM Appearance: Homogeneous, free flowing beige to yellow-beige
• PPM Appearance: Clear, yellow to amber no precipitate (ppt)
• pH (4.76% solution at 25°C): 6.8 - 7.4
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Good to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

PR1MA™ Agar, Granulated, High Gel Strength

CAS Number: 9002-18-0
Solubility: Water with Heat
Storage Temperature: Room Temperature

Purified agar with excellent properties for tissue culture and microbiological work.

• To prepare media, add 15 grams to 1 liter of water.

Research or further manufacturing use only, not for food or drug use.

• Appearance: White Cream Color, Average Particle Size Between 1-2 mm
• Granulometry (ASTM) 60 Mesh Sieve Size: Report %
• Ash: ≤ 6.5 %
• E.Coli: Negative
• Gel Point (1.5% Gel): 33 - 38°C
• Gel Strength (1.5% Gel): 600 - 1100 g/cm²
• Melting Point: 88 - 96°C
• Moisture : ≤ 15 %
• Molds and Yeasts: < 100 cfu/g
• pH: 6.0 - 8.0
• Total Plate Count: < 1000 cfu/g
• Viscosity (1.5 Solution @ 60 ^oC): ≤ 30 cps
• Water Absorption: ≤ 75 cc

Kappa is now PR1MA™ - Same great product, new name
Looking for something else? Email tech@midsci.com. If we can get it, we will add it to the website.

 

PR1MA™ Agarose, Low Melt Temperature

CAS Number: 9012-36-6
Solubility: Water
Storage Temperature: Room Temperature

Ideal gelling properties for use in cloning assays or for recovery of heat labile samples following electrophoresis.


Gel Strength, 1%: >200 g/cm2
Gelling Temperature: 26-30°C
Melting Temperature: <65°C
Sulfate: <0.10%
EEO: <0.10
DNase and RNase: None Detected


Research or further manufacturing use only, not for food or drug use.
 

• Appearance: White to off white powder
• DNase/ RNase: Not Detected
• EEO: ≤ 0.10 -Mr
• Gelling Temperature: 26 - 30 ^oC
• Gel Strength : ≥ 200 g/cm²
• Melting Temperature: ≤ 65 ^oC
• Moisture Content: ≤ 10 %
• Sulfate Content: ≤ 0.10 %

PR1MA™ Terrific Broth, Modified, Powder

CAS Number: 91079-40-2; 8013-01-2; 7778-77-0; 7758-11-4
Solubility: Water
Storage Temperature: Room Temperature

Enriched media for maintenance and propagation of E. coli and plasmid growth. Produces higher cell densities resulting in greater yields of DNA or recombinant problem. Glycerol is used as a carbon source with this formulation instead of glucose as it will not ferment to acetic acid.


Ingredients:

• Casein Digest Peptone: 12 g/L
• Yeast Extract: 24 g/L
• Dipotassium phosphate: 9.4 g/L
• Monopotassium phosphate: 2.2 g/L

• DCM Appearance: Homogeneous Free Flowing, Beige to Yellow Beige
• PPM Appearance: Clear Yellow to Amber ,No PPT
• pH (4.76% solution at 25°C): 6.8 - 7.4
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Good to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

PR1MA™ DTT [DL-Dithiothreitol] (Cleland's Reagent)]

Common Name: [DL-Dithiothreitol] (Cleland's Reagent)]
CAS Number: 3483-12-3
Molecular Weight: 154.25
Chemical Formula: C₄H₁₀O₂S₂
Solubility: Water
Storage Temperature: 2-8°C

Protective reagent for maintaining -SH groups in reduced state. DTT permeates the cell membrane protecting protein sulfhydryls thereby restoring enzyme activity lost by oxidation of the groups.


Research or further manufacturing use only, not for food or drug use.

• Appearance: A white free flowing powder, hygroscopic
• Solubility: Freely soluble in methanol, methylene chloride
• Clarity in water: 5% (w/v) solution in water is clear and colorless
• Identification by TLC: Rf value of the sample is comparable with that of the standard
• Melting Range: 37.0 - 44.0 °C
• Related Substance (UV Spectroscopy- Content of Oxidised DTT):  0.4 %
• Assay (By Iodimetry): ≥ 98.0 %
• Country of Origin : Report

PR1MA™  MOPS [3-(N-morpholino)-propanesulfonic acid]

Common Name: 3-(N-Morpholino)propanesulfonic acid
CAS Number: 1132-61-2
Molecular Weight: 209.3
Chemical Formula: C₇H₁₅NO₄S
Solubility: Water
Storage Temperature: Room Temperature

Molecular Biology Grade

• Heavy Metals: <5 ppm
• pKa: (20°C) 7.20 ± 0.2
• pH Range: 6.5-7.9

• Appearance: White crystalline powder
• Absorbance @250nm, 1M solution in H₂O: ≤ 0.1
• Absorbance 260nm: ≤ 0.2 nm
• Absorbance 280nm: ≤ 0.2
• Assay (Titration): ≥ 99.0 %
• Endotoxin : ≤ 0.5 EU/mg
• Heavy Metals : ≤ 5 ppm
• Identification: Matches the reference
• Iron (Fe): ≤ 5 ppm
• Loss on Drying (105 ^oC): ≤ 1.0 %
• Melting Point : 276.0 - 281.0 ^oC
• pH: 2.5 - 5.0
• pKa @ 20°C: 7.0 - 7.4
• Residue on Ignition: ≤ 0.2 %
• Solubility: Clear, colorless solution

PR1MA™ Yeast Nitrogen Base without Amino Acids

Solubility: Water
Storage Temperature: Room Temperature

For classifying yeasts based on amino acid and carbohydrates requirements. Contains all essential vitamins and inorganic salts necessary for the cultivaiton of yeasts. Excludes histidine methionine, tryptophan and carbon source. Also contains <1 mg/L each of vitamins and trace inorganic salts.


Research or further manufacturing use only, not for food or drug use.

• DCM Appearance: Free flowing powder, Homogeneous, Off white to cream
• PPM Appearance: Pale to light yellow (10X Solution)
• pH (0.67% Solution @25 ^oC): 5.2 - 5.6
• Microorganism Koleckera Apiculata ATCC 9774 (With Additives): Good to Excellent
• Microorganism Saccharomyces Cerevisiae ATCC 9080 (With Additives): Good to Excellent
• Microorganism Saccharomyces Cerevisiae ATCC 9763 (With Additives): Good to Excellent
• Microorganisms Candida Albicans ATCC 10231 (With Additives): Good to Excellent

PR1MA™ LB Agar, Low Salt Formula, Granulated [Lennox L Agar]

Common Name: Lennox L Agar
CAS Number: 91079-40-2, 8013-01-2, 7647-14-5, 9002-18-0
Solubility: Water with Heat
Storage Temperature: Room Temperature

For propagation and maintenance of E. Coli. Dissolve 32 grams in 1 Liter distilled water and adjust the pH to 7.2. Sterilize by autoclaving for 15 minutes.


Ingredients:

• Casein Peptone: 10 g/L
• Sodium Chloride: 5 g/L
• Yeast Extract: 5 g/L
• Agar: 12 g/L

• DCM Appearance: Homogeneous Free Flowing ,Light Beige
• PPM Appearance: Clear, no to light ppt, light yellow
• pH (2.0% Solution @ 25 ^oC): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Fair to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent
• Sodium Chloride: 5 g/l
• Yeast Extract: 5 g/L
• Agar: 12 g/l
• Casein Peptone: 10 g/L

PR1MA™ LB Agar, Low Salt Formula, Powder [Lennox L Agar]

Common Name: Lennox L Agar
CAS Number: 91079-40-2, 8013-01-2, 7647-14-5, 9002-18-0
Solubility: Water with Heat
Storage Temperature: Room Temperature

Molecular Biology Grade. For propagation and maintenance of E. Coli. Low Salt Formula.


Dissolve 35 grams in 1 Liter distilled water. Adjust the pH to 7.2 Sterilize by autoclaving for 15 minutes


Ingredients:

• Casein Peptone: 10 g/L
• Sodium Chloride: 5 g/L
• Yeast Extract: 5 g/L
• Agar: 15 g/L

Research or further manufacturing use only, not for food or drug use.

• Appearance: Powder
• pH: 6.8 - 7.2
• Solubility: Clear Solution

PR1MA™ LB Broth Powder [Lennox L Broth]

Common Name: Lennox L Broth
Solubility: Water
Storage Temperature: Room Temperature

For propagation and maintenance of E. Coli. Low Salt Formula. Molecular Biology Grade.


Dissolve 20 grams in 1 liter distilled water. Then adjust pH to 7.2. Heat and frequently agitate to completely dissolve. Sterilize by autoclaving for 15 minutes.


Ingredients:

• Tryptone: 10 g/L
• Yeast Extract: 5 g/L
• Sodium Chloride: 5 g/L

Research or further manufacturing use only, not for food or drug use.

• DCM Appearance: Homogeneous free flowing, light beige
• PPM Appearance: Clear, No to light ppt, light yellow
• pH (2% water) @25°C: 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Fair to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent
• Sodium Chloride: 5 g/L
• Yeast Extract: 5 g/L
• Casein Peptone: 10 g/L

PR1MA™ LB Broth 20 Gram Buffered Capsules [Lennox L Broth]

Common Name: Lennox L Broth
Solubility: Water
Storage Temperature: Room Temperature

RPI media is now available in convenient premeasured and pre-mixed capsules. Prebuffered media eliminates pH adjustments, dust and smells from the lab. Low Salt Formula. Molecular Biology Grade.


For propagation and maintenance of E.Coli:

• Add 1 capsule per liter of water.
• Heat and stir until capsule is completely dissolved.
• Buffer is set for 7.2 at 20°C using a 10mM solution of TRIS/TRIS HCl
• Sterilize by autoclaving for 15 minutes.

Ingredients:

• Tryptone: 10 g/L
• Yeast Extract: 5 g/L
• Sodium Chloride: 5 g/L

Research or further manufacturing use only, not for food or drug use.

• Appearance: Capsules
• Appearance of Solution: Prepared medium is yellow to gold and clear to moderately hazy
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Fair to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

PR1MA™  Luria Broth, High Salt Formula, Powder [Miller's LB Broth]

Common Name: Miller's LB Broth
CAS Number: 91079-40-2, 8013-01-2, 7647-14-5
Solubility: Water
Storage Temperature: Room Temperature

For propagation and maintenance of E. Coli. High Salt Grade. Molecular Biology Grade.

• Dissolve 25 grams in 1 liter distilled water
• Adjust the pH to 7.2
• Sterilize by autoclaving for 15 minutes

• Tryptone: 10 g/L
• Yeast: 5 g/L
• Sodium Chloride: 10 g/L

• DCM Appearance: Homogeneous free flowing, light beige
• PPM Appearance: Clear,no to light ppt, light yellow
• pH (2.5% H₂O @25 °C): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Good to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• BSE/TSE Free: BSE/TSE Free
• Country of Origin : USA

PR1MA™ Luria Broth 1 Gram Buffered Capsules [Miller's LB Broth]

Common Name: Miller's LB Broth
CAS Number: 91079-40-2; 8013-01-2; 7647-14-5; 77-86-1/1185-53-1
Solubility: Water
Storage Temperature: Room Temperature

High salt formula. Molecular Biology Grade. For maintenance and propagation of E. Coli.


RPI Media is now available in convenient pre-measured and pre-mixed capsules. Prebuffered media eliminates messy weighing and pH adjustments.


Simply dissolve 25 capsules per 1 Liter of distilled water. Water must be 37°C or warmer to completely dissolve gelatin capsule. Agitate. Sterilize by autoclaving for 15 minutes before use. Buffer is set for 7.0 ± 0.2 at 25°C using a 10mM solution of Tris/Tris HCl. pH will vary depending on temperature.


Ingredients:

• Tryptone: 10 g/L
• Sodium Chloride: 10 g/L
• Yeast Extract: 5 g/L
• Tris/Tris HCl: 1.5 g/L

Research or further manufacturing use only, not for food or drug use.

• Appearance: Capsules
• pH (2.5% H₂O @25 °C): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Growth
• Microorganism (E. coli ATCC 33694): Growth
• Microorganism (E. coli ATCC 33849): Growth
• Microorganism (E. coli ATCC 39403): Growth
• Microorganism (E. coli ATCC 47014): Growth
• Microorganism (E. coli ATCC 53868): Growth

PR1MA™ Miller's LB Agar Capsules

PR1MA™ Tween 20

Common Name: Polyoxyethylenesorbitan Monolaurate
CAS Number: 9005-64-5
Solubility: Water
Storage Temperature: Room Temperature

Fatty acid composition:

• Lauric acid: Approximately 50%
• Balance: Myristic, Palmitic and Stearic acids.

Shipping Weight: 1.2 lbs
Shipping Dimensions: 2.00 x 6.00 x 2.00

Specifications

Appearance: Yellow to amber viscous liquid
1,4-Dioxane: ≤ 10 ppm
Acid Value: ≤ 2.20 mg KOH/g
Color, Gardner: ≤ 5.0
Hydroxyl Value: 96 - 108 mgKOH/g
Saponification Value: 40.0 - 50.0 mg KOH/g
Water: ≤ 3.00 %

Research or further manufacturing use only, not for food or drug use.

PR1MA™ Tris-Acetate