PR1MA™  Luria Agar Granulated [Miller's LB Agar]

Common Name: Miller's LB Agar
CAS Number: 91079-40-2; 8013-01-2; 7647-14-5; 9002-18-0
Solubility: Water with Heat
Storage Temperature: Room Temperature

For propagation and maintenance of E. Coli.


Dissolve 37 grams in 1 Liter distilled water and adjust the pH to 7.2. Sterilize by autoclaving for 15 minutes.


Ingredients:

• Tryptone: 10g/l
• Sodium Chloride: 10g/l
• Yeast Extract: 5g/l
• Agar: 12g/l

• DCM Appearance: Homogeneous Free Flowing,Light Beige
• PPM Appearance: Clear, none to light ppt, light yellow
• pH (2.5 % Solution @25 ^oC): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Fair to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

PR1MA™  Peptone, Soy

 
CAS Number: 91079-46-8
Solubility: Water
Storage Temperature: Room Temperature

From papaic hydrolysis of soybean meal. Low NaCl content.
 

Research or further manufacturing use only, not for food or drug use.
 

• Amino Nitrogen: ≥ 2.2 %
• Ash: ≤ 15 %
• Loss on Drying: ≤ 6.0 %
• pH (2% water) @25°C: 6.5 - 7.5
• Total Nitrogen: ≥ 7.0 %
• Coliform: Negative
• E.Coli: Negative
• Molds and Yeasts: ≤ 100 cfu/g
• Salmonella: Negative /25g
• Standard Plate Count : ≤ 5000 cfu/g
• Broth Formulations (Growth Supporting Properties): Satisfactory
• Peptone Agar (Growth Supporting Properties): Satisfactory
• Country of Origin of Raw Material : Report
• Raw Material Species: Plant
• Country of Origin of Enzymes : Report
• Enzyme Species: Porcine
• Country Of Manufacture: Report

Pall® Ampoule Media for Microbiological Analysis

• Sterile Ampoule Media Pre-Measured for Maximum Efficiency and Convenience
• Meets Membrane Filter (MF) Technique Standard Method requirements with 2 mL ampoules*
• Maximizes efficiency with premixed and presterilized media
• Choose between plastic or glass ampoules for your applications
• Simplify pouring of the media with wide-mouth glass ampoules
• All plastic ampoule media is economically packaged with 50 ampoules per box

PR1MA™ LB Broth Powder [Lennox L Broth]

Common Name: Lennox L Broth
Solubility: Water
Storage Temperature: Room Temperature

For propagation and maintenance of E. Coli. Low Salt Formula. Molecular Biology Grade.


Dissolve 20 grams in 1 liter distilled water. Then adjust pH to 7.2. Heat and frequently agitate to completely dissolve. Sterilize by autoclaving for 15 minutes.


Ingredients:

• Tryptone: 10 g/L
• Yeast Extract: 5 g/L
• Sodium Chloride: 5 g/L

Research or further manufacturing use only, not for food or drug use.

• DCM Appearance: Homogeneous free flowing, light beige
• PPM Appearance: Clear, No to light ppt, light yellow
• pH (2% water) @25°C: 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Fair to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent
• Sodium Chloride: 5 g/L
• Yeast Extract: 5 g/L
• Casein Peptone: 10 g/L

PR1MA™ Terrific Broth, Modified, Granulated

CAS Number: 91079-40-2; 8013-01-2; 7778-77-0; 7758-11-4
Solubility: Water
Storage Temperature: Room Temperature

Enriched media for maintenance and propagation of E. Coli and plasmid growth. Produces higher cell densities resulting in greater yields of DNA or recombinant problem. Glycerol is used as a carbon source with this formulation instead of glucose as it will not ferment to acetic acid.


Ingredients:

• Casein Digest Peptone: 12g/L
• Yeast Extract: 24g/L
• Dipotassium phosphate: 9.5g/L
• Monopotassium phosphate: 2.2g/L

Research or further manufacturing use only, not for food or drug use.

• DCM Appearance: Homogeneous, free flowing beige to yellow-beige
• PPM Appearance: Clear, yellow to amber no precipitate (ppt)
• pH (4.76% solution at 25°C): 6.8 - 7.4
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Good to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

PR1MA™ Terrific Broth Modified Capsules

CAS Number: 91079-40-2; 8013-01-2; 7778-77-0; 7758-11-4
Solubility: Water
Storage Temperature: Room Temperature

Terrific Broth formulation packaged in easy to use 24 gram capsules. Formulation from powder.


Add 1 capsule per 500ml of water. Heat and stir until capsules are completely dissolved. Add 2ml of glycerol to mixture. Sterilize by autoclaving for 15 minutes.


Ingredients:

• Casein Digest Peptone: 12 g/L
• Yeast Extract: 24 g/L
• Dipotassium Phosphate: 9.4 g/L
• Potassium Phosphate: 2.2 g/L

Research or further manufacturing use only, not for food or drug use.

• Appearance: Water soluble gelatin capsules containing cream to beige free flowing powder.
• Appearance of Solution: Prepared medium is light to medium amber and clear
• pH (4.76% solution at 25°C): 7.0 - 7.4
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Good to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent
• Yeast Extract: 24.0 g/L
• Casein Peptone: 12.0 g/L
• Potassium phosphate dibasic: 9.4 g/L
• Potassium phosphate monobasic: 2.2 g/L
• Direction (Preparation): Add 2 capsules to one liter of purified water until evenly dispersed. Add 4ml of Glycerol to the mixture. Boil for one minute with stirring to completely dissolve. Distribute and autoclave at 121°C for 15 minutes.

PR1MA™ LB Broth 20 Gram Buffered Capsules [Lennox L Broth]

Common Name: Lennox L Broth
Solubility: Water
Storage Temperature: Room Temperature

RPI media is now available in convenient premeasured and pre-mixed capsules. Prebuffered media eliminates pH adjustments, dust and smells from the lab. Low Salt Formula. Molecular Biology Grade.


For propagation and maintenance of E.Coli:

• Add 1 capsule per liter of water.
• Heat and stir until capsule is completely dissolved.
• Buffer is set for 7.2 at 20°C using a 10mM solution of TRIS/TRIS HCl
• Sterilize by autoclaving for 15 minutes.

Ingredients:

• Tryptone: 10 g/L
• Yeast Extract: 5 g/L
• Sodium Chloride: 5 g/L

Research or further manufacturing use only, not for food or drug use.

• Appearance: Capsules
• Appearance of Solution: Prepared medium is yellow to gold and clear to moderately hazy
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Fair to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

PR1MA™ Nematode Growth Medium

Common Name: Nematode Growth Medium

Solubility: Water with Heat

Storage Temperature: Room Temperature

A standard formulation for use in Nematode growth studies.

Contains:

Agar 17.5 g/l
Sodium Chloride 3.0 g/l
Peptone 2.5 g/l
Cholesterol 0.005 g/l
Shipping Dimensions: 3.50 x 3.75 x 3.50
Shipping Weight: 1.2 lbs
 

Research or further manufacturing use only, not for food or drug use.

Specifications

• Appearance: Light tan to pale yellow powder
• Solubility: Clear Solution
• pH (2.3% Solution @25 oC): Report
• Agar: 17.5 g/l
• Sodium Chloride: 3.0 g/l
• Peptone: 2.5 g/l
• Cholesterol: 0.005 g/l

PR1MA™ SOB Broth

Solubility: Water
Storage Temperature: Room Temperature

SOB Broth is recommended for use in cultivation of recombinant strains of E. Coli. It is a nutritionally rich growth medium for use in the preparation and transformation of competent cells.


Add 28 grams of Broth in 1 liter of distilled water. Heat gently to dissolve the medium completely. Dispense in tubes and sterilize by autoclaving for 15 minutes.


Ingredients:

• Casein Enzymic Hydrolysate: 20 g/L
• Magnesium Sulfate: 2.4 g/L
• Yeast Extract: 5 g/L
• Potassium Chloride: 0.186 g/L
• Sodium Chloride: 0.5 g/L
• Final pH(25°:C): 7.0+0.2

• DCM Appearance: Homogeneous Free Flowing,Light Beige
• PPM Appearance: Clear, no to light ppt, light yellow
• pH (2.8 % Solution @25 ^oC): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Good to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent

PR1MA™ Miller's LB Agar Capsules

Agar, Bacteriological Grade

CAS Number: 9002-18-0

Solubility: Water with Heat

Storage Temperature: Room Temperature

Mix 15 grams per liter in Molecular Biology grade water.

Shipping Weight: 1.4 lbs

Shipping Dimensions: 3.50 x 7.00 x 3.50

Research or further manufacturing use only, not for food or drug use.

Appearance: White to Cream Powder

Gel Strength (1.5% gel, 20°C): 600 - 1100 g / cm³

Loss on Drying: Report %

PR1MA™ LB Broth 1 Gram Buffered Capsules [Lennox L Broth]

Common Name: Lennox L Broth
Solubility: Water
Storage Temperature: Room Temperature

RPI media is now available in convenient premeasured and pre-mixed capsules. Prebuffered media eliminates pH adjustments, dust and smells from the lab. For propagation and maintenance of E.Coli. Low Salt Formula. Molecular Biology Grade.


Add 20 capsules per liter of water. Heat and stir until capsule is completely dissolved. Buffer is set for 7.2 at 20°C using a 10mM solution of TRIS/TRIS HCl. Sterilize by autoclaving for 15 minutes.


Ingredients:

• Tryptone: 10 g/L
• Yeast Extract: 5 g/L
• Sodium Chloride: 5 g/L

Research or further manufacturing use only, not for food or drug use.

• Appearance: Capsules
• Appearance of Solution: Prepared medium is yellow to gold and clear to moderately hazy
• pH (2.15% solution @25°C): 6.8 - 7.2
• Microorganism (E. coli ATCC 23724): Good to Excellent
• Microorganism (E. coli ATCC 33694): Good to Excellent
• Microorganism (E. coli ATCC 33849): Fair to Excellent
• Microorganism (E. coli ATCC 39403): Good to Excellent
• Microorganism (E. coli ATCC 47014): Good to Excellent
• Microorganism (E. coli ATCC 53868): Good to Excellent