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Accuris IsoPure™ 96 & IsoPure™ Mini | Automated Extraction Systems
IsoPure™ 96
- Operates as a stand-alone system, or connects to PC for programming
- 8-position carousel for deep well sample plates
- Intuitive programming and operation on a user-friendly touchscreen
- Reliable and automated processing of up to 96 samples
The IsoPure™ 96 from Accuris Instruments is a high-throughput automated extraction system that improves the efficiency and reproducibility of magnetic bead extractions. Capable of processing up to 96 samples at one time, the instrument is easy to set up and program. Mixing, magnetic bead transfer, washing, and elution steps are performed automatically, saving valuable hands-on time. The complete purification process can be finished in as little as 10 minutes (depending on the reagent kit and the complexity of the program.)
IsoPure™ Mini
- Compact design takes up only 8 x 10 inches of bench space
- Intuitive programming on a user-friendly Android tablet app
- Higher yields and reproducibility than manual extraction methods
- Fast and reliable sample processing
The IsoPure™ Mini from Accuris Instruments is an extremely compact, automated extraction system that improves the efficiency and reproducibility of magnetic bead extractions. Capable of processing up to 16 samples at one time, the instrument is easy to set up and program. Mixing, magnetic bead transfer, washing, and elution steps are performed automatically, saving valuable hands-on time. The complete purification process can be finished in as little as 10 minutes (depending on the reagent kit and the complexity of the program.)
Specifications
| AP1096 | AP1016 | |
| Capacity / Sample Volume | 96 Samples / 50 µL to 1000 µL | 16 Samples / 50 µL to 1000 µL |
| Magnetic Bead Recovery Rate | >95% | >95% |
| Lysis Temperature Range | RT to 120°C | RT to 120°C |
| Elution Temperature Range | RT to 120°C | RT to 120°C |
| Display | 7.0 inch color screen | 4.3 inch color screen |
| Programming Control | Touch screen/mouse, or PC connection | Touch screen/mouse, or PC connection |
| Program Memory | 100 programs | 100 programs |
| Communication Ports | USB, Ethernet | USB, Ethernet |
| Contamination Control | UV Sterilization | UV Sterilization |
| Electrical Input | Universal 120 to 240VAC | Universal 120 to 240VAC |
| Dimensions | 22 x 24.5 x 19.7 in 56 x 62 x 50 cm | 7.9 x 10.2 x 11.8 in 20 x 26 x 30 cm |
PR1MA™ β - Agarase
PR1MA™ β - Agarase has a high tolerance to inhibitors in electrophoretic buffers such as TBE and TAE, eliminating the need to perform a buffer exchange step before digestion. PR1MA™ β - Agarase has higher thermal stability than other commercially available β - agarases, with a broad range of activity between 42°C and 50°C. Higher temperature digestion reduces the chances of residual agarose gelling at the end of the reaction, resulting in higher recovery of DNA or RNA.
Benefits
- Complete digestion of agarose, with no agarose fragments, left after the digestion
- Obtain DNA or RNA faster - no buffer exchange needed, with direct digestion in TAE or TBE
- Concentration: 1000 u / mL
- Operating temperature: 42°C to 50°C
- Recommended temperature: 50°C
- Storage temperature: -20°C
Storage Buffer
- 50% glycerol
- 50 mM Tris-HCI
- 50 mM KCI
- 1 mM DTT
- 0.1 mM EDTA
- pH = 7.5
*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.
Proudly made in the USA! Click here for more made in America products!
PR1MA™ RNase Inhibitor
PR1MA™ RNase Inhibitor (RI) is a 50 kDa protein that specifically inhibits RNases A, B, and C by binding noncovalently in a 1:1 ratio at high affinity. It can be used in isothermal amplification and molecular diagnostic assays, cDNA synthesis, and other applications where RNA stability is important. It is ineffective against RNase1, T1, S1 Nuclease, or RNase H. It has no inhibition of polymerase activity when used with Taq DNA polymerase, AMV, M-MuLV, HIV reverse transcriptases, or phage RNA polymerases.
Storage Buffer
- 50% glycerol
- 10 mM Tris-HCl
- 50 mM KCl
- 8 mM DTT
- pH = 7.5
*To prevent the release of ribonuclease from RNase Inhibitor, temperatures greater than 50°C and high concentrations of denaturing reagents such as urea should be avoided.
*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.
Proudly made in the USA! Click here for more made in America products!
PR1MA™ MS2 Phage
PR1MA™ MS2 is an E. coli bacteriophage with a single-stranded RNA genome of 3569 nucleotides protected from nuclease degradation by a capsid of 180 coat protein monomers. This virus is a Biosafety Level 1 organism, not pathogenic to humans. These properties make MS2 phage useful as a process control in nucleic acid-based amplification techniques like RT-PCR, RT-LAMP, particularly those that involve viral RNA extraction.
- Biosafety level 1
- Lysis: 65°C for 20 minutes or by standard RNA extraction
- Storage temperature: 4°C
Buffer composition
- 10 mM Tris-HCl
- 0.1 mM EDTA
- pH = 8.0
*These products are intended for research use only, not for therapeutic or diagnostic purposes in humans or animals. The safety and efficacy of these products in diagnostic or other clinical uses has not been established.
Proudly made in the USA! Click here for more made in America products!
MagBio HighPrep™ Viral/Pathogen DNA/RNA Kit
Magnetic beads based kit for rapid isolation of viral, bacterial and fungal nucleic acids from whole blood, serum, plasma, saliva and other body fluids.
- RT-qPCR; RT-PCR, PCR
- One-Step RT-qPCR
- Virus detection, genotyping
- Viral load monitoring,
- OPTIMIZED FOR ISOLATION from FUNGAL, BACTERIAL and VIRAL samples.
- Rapid and reliable purification of nucleic acids
- Adaptable to various automated liquid handling workstations
- No toxic organic solvents
The HighPrep™ Viral/Pathogen DNA/RNA kit is designed for rapid and reliable isolation of viral, bacterial and fungal nucleic acids from whole blood, serum, plasma, saliva and other body fluids as well as nasopharyngeal swabs soaked in virus transport media or other buffers. This kit is highly efficient in vIral nucleic acid isolation and the extracted RNA (and DNA) is suitable for direct use in most downstream applications such as one-step RT-qPCR, RT-PCR, PCR, nucleic acid amplification, cloning, sequencing, and enzymatic reactions. The kit can be used in low throughput manual workflows and is also adaptable to majority of the liquid handling workstations in the market.
, Beads
The I-Blue MINI Plasmid Kit was designed for rapid isolation of plasmid DNA from 1-7 mL of cultured bacterial cells. I-Blue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. Typical yields are 20-35 µg for high-copy number plasmid or 3-10 µg for low-copy number plasmid from 4 mL of cultured bacterial cells. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 15 minutes. The purified plasmid DNA is ready for use in restriction enzyme digestion, ligation, PCR, and sequencing reactions.
Advantages
- Sample: 1-7 mL of cultured bacterial cells
- Yield: Up to 50 µg of pure plasmid DNA
- Format: Plasmid spin column
- Operation Time: Within 15 minutes
- Elution Volume: 30-100 µL
- Kit Storage: Dry at room temperature (15-25°C) for up to 1 year; PD1 and RNase A mixture should be stored at 2-8°C for up to 6 months
Quality Control
The quality of the I-Blue MINI Plasmid Kit is tested on a lot-to-lot basis by isolating plasmid DNA from a 4 mL overnight E. coli (DH5α) culture containing plasmid pBluescript (A600 > 2 U/mL). Following the purification process, a yield of more than 20 µg is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid DNA (1 µg) is used in EcoRI digestion, and analyzed by electrophoresis.
The I-Blue MINI Plasmid Kit was designed for rapid isolation of plasmid DNA from 1-7 mL of cultured bacterial cells. I-Blue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. Typical yields are 20-35 µg for high-copy number plasmid or 3-10 µg for low-copy number plasmid from 4 mL of cultured bacterial cells. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 15 minutes. The purified plasmid DNA is ready for use in restriction enzyme digestion, ligation, PCR, and sequencing reactions.
Advantages
- Sample: 1-7 mL of cultured bacterial cells
- Yield: Up to 50 µg of pure plasmid DNA
- Format: Plasmid spin column
- Operation Time: Within 15 minutes
- Elution Volume: 30-100 µL
- Kit Storage: Dry at room temperature (15-25°C) for up to 1 year; PD1 and RNase A mixture should be stored at 2-8°C for up to 6 months
Quality Control
The quality of the I-Blue MINI Plasmid Kit is tested on a lot-to-lot basis by isolating plasmid DNA from a 4 mL overnight E. coli (DH5α) culture containing plasmid pBluescript (A600 > 2 U/mL). Following the purification process, a yield of more than 20 µg is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid DNA (1 µg) is used in EcoRI digestion, and analyzed by electrophoresis.
PCR DNA Clean-up Kit
The 96-well PCR Clean-Up/DNA Extraction Kits provide a high-throughput, rapid and economical method to purify DNA fragments. Chaotropic salt is used to denature enzymes and in this condition, DNA fragments are bound by the glass fiber matrix in each well of the plate. Once the contaminants have been removed, the purified DNA is eluted by a low salt elution buffer or water. Salts, enzymes, and unincorporated nucleotides are effectively removed from reaction mixtures without toxic phenol extraction or alcohol precipitation. This entire protocol can be completed in 30-40 minutes, and the eluted DNA is ready to use in restricted digestion, ligation, PCR, and sequencing reactions.
PCR DNA Clean-up Kit
The 96-well PCR Clean-Up/DNA Extraction Kits provide a high-throughput, rapid and economical method to purify DNA fragments. Chaotropic salt is used to denature enzymes and in this condition, DNA fragments are bound by the glass fiber matrix in each well of the plate. Once the contaminants have been removed, the purified DNA is eluted by a low salt elution buffer or water. Salts, enzymes, and unincorporated nucleotides are effectively removed from reaction mixtures without toxic phenol extraction or alcohol precipitation. This entire protocol can be completed in 30-40 minutes, and the eluted DNA is ready to use in restricted digestion, ligation, PCR, and sequencing reactions.
MagBio HighPrep™ Insect DNA Kit
Magnetic beads based kit designed to extract high-quality genomic DNA from insects, and arthropods (from one leg or wing to the whole body).
- PCR, Real-time PCR
- Cloning, genotyping
- Sequencing
- Insecticide resistance studies
- Efficient isolation of PCR-quality DNA from insects, and arthropods
- No organic solvents
- Allows field epidemiologic research
- Magnetic beads based chemistry adaptable to automation
The HighPrep™ Insect DNA Kit is a high quality paramagnetic bead-based genomic DNA extraction kit used to isolate gDNA from a variety of insect-derived materials (wings, legs, whole body), and from different insect species, such as mosquitoes, flies, ants, and others. The HighPrep™ Insect DNA Kit is based on a fast and simple procedure which can either be performed manually or automated - up to 96 samples of tissues including legs and wings, can be processed in less than an hour.
, Beads
MagBio HighPrep™ RNA Elite Clean-up System
Magnetic beads based reagent for manual and automated clean-up or concentration of RNA (including miRNA, siRNA, aRNA) and single-stranded cDNA after enzymatic reactions or prior sequencing.
- cDNA synthesis
- RT-PCR
- Sequencing
- In vitro transcription
- RNA probe synthesis
- miRNA and siRNA preparation
- Rapid and reliable clean-up of ss cDNA and RNA, including miRNA, siRNA, and aRNA
- Efficient purification of both large and small RNAs, and cDNA
- Complete removal of salts, unincorporated primers, and nucleotides
- No centrifugation step, no filtration step
- Adaptable to your current liquid handling workstation
, Beads
MagBio cfKapture™ Kit
Magnetic bead-based DNA extraction kit designed to isolate circulating cell free DNA (cfDNA) from human plasma and serum.
- Liquid biopsy research
- NIPT genomic research
- Cancer genomic research
- Transplant monitoring
- Efficient Isolation of nucleic acids, with high input and low elution volumes
- No carrier RNA
- Low sample volume processing
- Efficient recovery of small sized fragmented DNA
- No organic extraction or ethanol precipitation
, Beads
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