PR1MA™ Autoradiography and Chemiluminescence Film for Western Blots

One autoradiography film for ALL your needs!

Many companies sell film solely on price without considering your needs. Some offer many different films making it difficult to decide what is best for your needs.

MIDSCI™ narrowed the Autoradiography Film field to PR1MA™ Blue Sensitive Film. It will work outstandingly well for almost 100% of all molecular labs' needs.
 

• PR1MA™ Autoradiography Film is double emulsion
• High signal sensitivity
• For autoradiography of ³²P, ³³P, ³⁵S, ¹²⁵I, and ¹⁴C
• Excellent lot-to-lot consistency
• Exceptional clarity with extremely low backgrounds
• Consistently superior results for exposure of blotting experiments and sequencing gels
• Publishable grade autoradiographs
• Optimized for chemiluminescence
• Film works with calcium tungstate or blue rare earth screens
• For use manually or with automatic processing
• 100 sheets per box
• Available in 3 sizes: 5 x 7 in., 8 x 10 in., and 14 x 17 in.

Specifications

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PR1MA™ Rapid Coomassie Stain

Proudly made in the USA! Click here for more made in America products!
 

Rapid, Commassie, Stain, PR1MA, MIDSCI, Laboratory Supplies, High-Quality, Research, Consumables, Reagents, Equipment, Customer Service, Quick Shipping, Satisfaction Guarantee, Personalized Solutions, Universities

PR1MA™ Protein Gel-Loading Dye for SDS-PAGE

Protein, Gel-Loading, Dye, SDS-PAGE,PR1MA, MIDSCI, Laboratory Supplies, High-Quality, Research, Consumables, Reagents, Equipment, Customer Service, Quick Shipping, Satisfaction Guarantee, Personalized Solutions, Universities

Gel Crusher™ Spin Column

• Creates uniform gel fragments with no chunks
• Fits in standard microtubes
• Increases DNA yield
• For use in molecular cloning, PCR, restriction digestions, sequencing, and labeling protocols
• Fits 2 mL receiver tubes 

MTC Bio’s Gel Crusher™ Spin Columns are designed to replace labor-intensive gel fragmentation steps (crush+soak method, etc.). Commonly used in molecular cloning, PCR, restriction digestions, sequencing, and labeling protocols, these spin columns can be used to fragment gel slices (typically cut with a gel extractor or razor blade) into smaller pieces.

These smaller uniform pieces will produce greater yields and can be easily pipetted using wide bore tips into an NaCl solution. This reduces the time to create a fine slurry for further elution, filtration, incubation, etc. Place the Gel Crusher™ column in the 2 mL microtube and centrifuge the column/tube assembly in a standard benchtop microcentrifuge for 5 minutes at maximum speed (generally approximately 15,000 x g).

The gel will be forced through the 600 uM holes into the 2 mL receiver tube. The crushed fragments will be small enough to be aspirated/dispensed through a pipette tip.

Reversible Racks

• Alphanumeric markings and are supplied in packs of 5.
• Choose 60 well (5 x 12) or 96 well (8 x 12) formats
• Reversible racks, accept 0.5 and 1.5 to 2.0 mL microtubes
• Alphanumeric marking on each well
• Made of polypropylene   

Azure ChemiWriter ECL Pen

Chemiluminescent pen for blot annotation

The Azure ChemiWriter ECL Western blot annotation pen is a chemiluminescent marker that enables you to write or draw on your transfer membranes, resulting in a chemiluminescent signal on your markings once substrate is added. Contains a reagent that reacts with HRP substrates to produce chemiluminescence and also a blue dye to allow you to see markings as you write on the Western blot.

Use for tracing visible protein ladders or making identifying marks on your Western blots. Pen has two tips on each end: one fine-tipped for thin markings and one thicker for depositing more of the reagent.

Quantity: 1 pen

Features

Enables you to write or draw on your transfer membranes. A chemiluminescent signal will be detectable from your markings once chemiluminescent substrate is added.

• Use for tracing visible protein ladders or making identifying marks on your blots. Pen has two tips on each end: one fine-tipped for thin markings and one thicker for depositing more of the reagent.
• There are two heads to the pen, a fine point tip and a thicker chisel point tip.
• We recommend using the fine point tip to annotate the blot and mark protein ladders for detection.
• The chisel point of the pen can be used to deposit an increased amount of reagent on the blot for more intense signal.
• For best results we recommend annotating your blot after transfer and before starting your Western blotting protocol.
• Once annotated, the blot may be stored dry or wet, refrigerated or at room temperature.
• Storage: pen should be stored tightly closed at room temperature. ChemiWriter ECL reagent and blue ink markings on the blot are stable for at least three months.

Azure Western Blot Quenching Sheets

Background quenching sheets for use with fluorescent or chemiluminescent samples

Use these quenching sheets under your blot when imaging fluorescent or chemiluminescent Western blots. Sheets reduce background autofluorescence or background signal from contamination in shared imagers, improving the quality of your image.

• For use with fluorescent or chemiluminescent samples.
• Size: 9 x 12.5 cm
• Quantity: 10 Sheets

Features

Use these quenching sheets under your blot when imaging fluorescent or chemiluminescent Western blots.

The sheets reduce background autofluorescence or background signal from contamination in shared imagers, improving the overall quality of your image.

Azure Nitrocellulose Membranes

Pre-cut Nitrocellulose membrane for Western blotting

Low-fluorescence Nitrocellulose membranes are available in both 0.45 μm and 0.22 μm pore sizes to accommodate larger and smaller proteins, respectively. We recommend using the .45 µm pore size for the most analytical blotting: protein, ssDNA, and RNA transfers.

• Low molecular weight proteins
• Typically used for chemiluminescence
• Not ideal for stripping
• Excellent binding properties

Quantity: 10 membranes each   
 
 
 

Azure PVDF Membranes

Pre-cut PVDF membranes for Western blotting applications

Ideal for chemiluminescent and colorimetric Western blotting. Pre-cut to save you time in the lab. These low-fluorescence PVDF membranes reduce background noise and nonspecific protein binding for improved sensitivity. Available in three different pre-cut sizes.

Quantity: 10 membranes per package

Features

Low-fluorescence PVDF membranes reduce background noise for improved sensitivity. PVDF (polyvinylidene difluoride) membranes are commonly used for immunoblotting techniques, like Western blots. During the blotting process, samples are transferred from the protein gel onto a PVDF membrane by electroblotting. From there they can be used for protein sequencing.

• High binding capacity is great for low-expressing proteins
• Typically used for fluorescence
• High protein retention for stripping and reprobing

Azure Blotting Paper for Western Blotting

Pre-cut blotting paper for Western blot transfer

Forget about measuring and cutting blotting paper to the size of your mini-gels. Use these pre-cut papers to assemble your transfer “sandwiches”. Pre-cut blotting papers save time and reduce waste for the easiest Western blotting workflow.

Qty: 60 sheets

Blotting papers are cut to completely cover the gels and membranes.

For 8x10cm gels: blotting papers available in 9x13cm

For 7x9cm gels: blotting papers available in 8x10cm

Features

Pre-cut blotting papers save time and reduce waste for the easiest Western blotting workflow. Forget about measuring and cutting blotting paper to the size of your mini-gels! Use these pre-cut papers to assemble your transfer “sandwiches”.

Blotting papers come pre-cut to completely cover the gels and membranes.   
 
 
 

Azure Flash Western Chemiluminescence Kit

Kit includes all reagents necessary for performing ten chemiluminescent Western blots using the Flash Western protocol

Each kit includes:

• 10x PVDF Membranes
• 100mL Chemi Blot Blocking Buffer
• 500μL Flash HRP Conjugate
• 250mL Flash Wash Buffer
• 100mL Radiance ECL Substrate

Features

Time savings using Azure Flash Western Chemiluminescent Kit. Flash Western graph includes use of Azure Transfer Buffer in light blue.

The Azure Flash Western Kit uses a proprietary set of reagents to achieve complete membrane blocking, rapid hybridization of antibodies, and enhancement of chemi signal in less than an hour of blotting time. The included transfer buffer cuts wet transfer time down to 15 to 30 minutes for a total assay time of two hours from gel to imaging.

• Fast protocol with few hands-on steps
• Antibody stabilization for enhanced chemi signal
• Low background and increased signal-to-noise ratios
• Increased detection sensitivity and linear dynamic range

Hoefer® Mighty Small Precast Gels

Hoefer® Mighty Small precast gels are high-performance mini polyacrylamide gels commonly used for protein separation. Utilizing the Bis-Tris buffer system, they effectively minimize protein modifications during electrophoresis, resulting in sharper and clearer protein bands with higher resolution.

• High loading capacity: 50 uL max. sample volume
• High resolution: Upgraded bis-Tris system for clearer, sharper separation bands and higher resolution
• Ready to use and easy to store: No solution preparation or gel casting is required, saving time. Store at 2 to 8ºC
• Good Repeatability: Fully automated gel casting process, minimal batch-to-batch variation, high stability, and good repeatability
• Highly effective: Max 40 min for electrophoresis
• Safe and non-toxic: No hazardous reagents need to be handled 

Compatible with Hoefer® Mighty Small II Vertical Electrophoresis Systems and the Hoefer® miniVE Vertical Protein Electrophoresis and Blotting Systems.  

Specifications
 

Item #	MSGT	MSGF
Plate Size	3.94 x 3.15 In. | 10 x 8 cm
Gel Size	3.35 x 2.76 In. | 8.5 x 7 cm, 1mm thickness
System/Buffer	Bis-Tris System, MOPS-SDS Buffer	Bis-Tris System, MOPS Buffer
Wells	12-well, 15-well
Storage	Store at 2 to 8°C. (35.6 to 46.4ºF) Do not freeze.
Concentration	Gradient Gels: 4 to 12%, 4 to 20% | Homogenous Gels: 10%, 12%
Loading Vol.	For 12 wells: max 50 uL (recommended: ≤ 25 uL) | For 15 wells: max 30 uL (recommended: ≤ 15 uL)

 

Glow-Writer Pen

Fine-Point Phosphorescent Marking Pen for Laboratory Autography

• Fine Point Nib- Provides fine writing and greater information density
• Specially Designed- Ideal glow intensity and afterglow duration helps prevent over-andunder exposure
• No Ink Clogs- Low ink viscosity and porous nib prevents ink from clogging