ILP Deep Well Round Well, U-Bottom Glass Coated Plates – 96

Glass coated, polypropylene microplates. The 200 nm thick coating of silicone dioxide creates a cradle of non-binding and inert glass to protect your sample.

• SiO2 coating helps to:
• Reduced binding
• Increase chemical resistance
• Protect the same from any potential compounds leached from the plastic.

Plate design

• Mid-height to maximize storage, utilizing 30-40% less vertical space, allowing for more storage in freezers, automated loaders, under the hood, and other locations where space is at a premium
• Automation friendly design – sturdy and warp resistant with ultra-flat surfaces and rigid construction
• Stacking lugs to help prevent toppling
• Raised rims protect from cross contamination, minimize sample loss during storage, and make for enhanced sealing with adhesive films, silicone mats, or heat sealing films
• Individually wrapped in medical grade Tyvek peel-packs
• Made from Virgin Polypropylene; no mold release agents or regrind
• RNase, DNase, DNA, and Pyrogen Free Certification

Specifications
 

Number of Wells	96
Number of Plugs	96
Plug Shape	Round – U
Well Shape Top	Round
Well Shape Bottom	Round – U
Material	USP Class VI Polypropylene
Adhesive Material	SiO2; 0.0079 Mil
Color	Natural
Alphanumeric	Molded in
Compliance	ANSI/SBS 6-2012
Surface Treatment	SiO2
Certified	DNase-/RNase-/Pyrogen-Free
Overall Height	1.30 In. | 33.00 mm
Well Depth	1.21 In. | 30.84 mm
Overall Length	5.03 In. | 127.78 mm
Adhesive Thickness	SiO2; 0.0079 Mil
Width	3.37 In. | 85.48 mm
Top Width	3.24 In. | 82.24 mm
Well Diameter Bottom	0.16 In. | 4.12 mm
Well Diameter Top	0.28 In. | 7.00 mm
Top Length	4.90 In. | 124.53 mm
Well Bottom To Deck	0.05 In. | 1.30 mm
Max Vol.	1.22 mL
Working Vol.	1.1 mL
Vol. With Mat Lid	1.0 mL
Plug Depth	1.21 In. | 30.84 mm
Plug Diameter	0.16 In. | 4.12 mm
Autoclavable	121ºC at 15 psi, 15 min. (249.8ºF)
Max RCF	4,000 RCF
Max Temp.	120ºC (248ºF)
Min Temp.	-80ºC (-112ºF)

 

ILP Deep Well Round Well, V-Bottom Glass Coated 33 mm Plates - 96
  

These round deep-well V-bottom glass-coated plates combine working volume with an automation-friendly design. Sturdy and warp-resistant design with ultra-flat surfaces and rigid construction make this plate ideal for high throughput screening, sample collection, compound storage, screening, and combinatorial chemistry applications. It is compatible with stackers, grippers, heater/cooler blocks, and other instruments that comply with the ANSI/SLAS 2004-1 (SBS) footprint.

Raised rims on each well help protect from cross-contamination, minimize sample loss during storage, and make for enhanced sealing with adhesive films, silicone mats, or heat-sealing films. Manufactured of inert, chemically resistant, and exceptionally clear virgin polypropylene. Made from medical grade virgin polypropylene (no mold release agents or regrind); certified RNase, DNase, DNA, and Pyrogen Free.

• Uniform wall thickness
• Easy well identification with molded-in alpha-numeric position markings
• Raised rims for easy and secure sealing with adhesive films, silicone mats, or heat-sealing films
• Raised rims help protect from cross contamination

Specifications
 

Number of Wells	96
Number of Plugs	96
Plug Shape	Conical -V
Well Shape Top	Round
Well Shape Bottom	Conical -V
Material	USP Class VI Polypropylene
Adhesive Material	SiO2; 0.0079 Mil
Color	Natural
Alphanumeric	Molded in
Compliance	ANSI/SBS 6-2012
Surface Treatment	SiO2
Certified	DNase-/RNase-/Pyrogen-Free
Overall Height	1.30 In. | 33.00 mm
Well Depth	1.21 In. | 30.84 mm
Overall Length	5.03 In. | 127.78 mm
Adhesive Thickness	SiO2; 0.0079 Mil
Width	3.37 In. | 85.48 mm
Top Width	3.24 In. | 82.24 mm
Well Diameter Bottom	0.16 In. | 4.12 mm
Well Diameter Top	0.28 In. | 7.00 mm
Top Length	4.90 In. | 124.53 mm
Well Bottom To Deck	0.05 In. | 1.30 mm
Max Vol.	1.1 mL
Working Vol.	1.0 mL
Vol. With Mat Lid	800 uL
Plug Depth	1.21 In. | 30.84 mm
Plug Diameter	0.16 In. | 4.12 mm
Autoclavable	121ºC at 15 psi, 15 min. (249.8ºF)
Max RCF	4,000 RCF
Max Temp.	120ºC (248ºF)
Min Temp.	-80ºC (-112ºF)

 

Film-Sealing Paddles

Hoefer® Mighty Small Transfer Tank

The TE22 Mighty Small Transfer Tank quickly and efficiently transfers proteins and nucleic acids from small gels to nylon, nitrocellulose or PVDF membranes. Maximum Gel Size 9 x 10 cm.

• Transfer as many as four small gels, up to 9 x 10 cm in less than an hour
• Uniform and strong electric field—supports efficient and even transfers
• Color-coded, easy to assemble and load cassettes—ensures proper orientation during transfer
• Superior tank design allows the cassettes to apply equal pressure across the stack - prevents gel distortion
• Built-in ceramic heat exchanger—provides excellent temperature control when used with an external cooling water bath
• Built-in alumina-covered cooling channel provides excellent temperature control with no more than a 5°C temperature increase during a typical run

Hoefer® Mighty Small Transfer Tank Includes

• Lower Chamber with Heat Exchanger, 1/ea
• Safety Lid with High Voltage Leads, 1/ea
• Electrode Panels, 2/pk
• Cassettes, 4/pk
• Foam Sponges, 6 mm thick, 4/pk
• Foam Sponges, 3 mm thick, 8/pk
• Cassette Hook, 1/ea
• Blotter Paper (9 x 10.5 cm), 25 sheets/pk

Hoefer® Mini Electrophoresis and Western Blot Package

Specifications
 

Capacity	Up to four 9 x 10 cm gels
Max. Power	100 V, 500 mA, 50 W
Max. Temp.	113ºF | 45°C
Indoor Use	39.2 to 104ºF | 4 to 40°C
Humidity	Up to 80%
Dims (D x W x H)	6.50 x 5.51 x 9.45 In. | 16.5 x 14 x 24 cm
Weight	4.441 lb | 2.00 kg

Hoefer® Standard Transfer Tank

The TE42 Standard Transfer Tank quickly and evenly transfers proteins and nucleic acids from polyacrylamide or agarose gels onto nylon, nitrocellulose, or PVDF membranes. The four slot design of the TE42 enables either cooled or non-cooled protein or nucleic acid transfers. The optional heat exchange module is easily incorporated into the TE42 enabling transfer of up to 2 gels simultaneously. When used without the optional heat exchange module, up to four gels can be transferred at one time.

• Uniform and strong electric field—supports efficient and even transfers
• Color-coded, easy to assemble cassettes—ensures proper orientation during transfer
• Superior tank design allows the cassettes to apply equal pressure across the stack - prevents gel distortion
• Optional TE47 heat exchanger—provides excellent buffer temperature control when used with an external cooling water bath
• Heat exchanger from an SE600 series electrophoresis unit can be substituted for the TE47 heat exchanger. 

Hoefer® Standard Transfer Tank Includes

• Lower Chamber, 1/ea
• Safety Lid with High Voltage Leads, 1/ea
• Electrode Panels, 2/pk
• Cassettes, 2/pk
• Foam Sponges, 6 mm thick, 2/pk
• Foam Sponges, 3 mm thick, 4/pk
• Cassette Hook, 1/ea
• Blotter Paper (14.5 x 21.5 cm), 25 sheets/pk

Specifications
 

Capacity	Up to four 15 x 21 cm gels (w/out cooling) Up to two 15 x 21 cm gels (w/ cooling)
Max. Power	100 V, 2 A, 200 W
Max. Temp.	113ºF | 45°C
Indoor Use	39.2 to 104ºF | 4 to 40°C
Humidity	Up to 80%
Dims (D x W x H)	12.01 x 3.15 x 5.12 In. | 30.5 x 8 x 13 cm

Hoefer® Standard Transfer Tank with Cooling Chamber

TE62 Standard Transfer Tank with Cooling Chamber quickly and evenly transfers proteins and nucleic acids from polyacrylamide or agarose gels onto nylon, nitrocellulose, or PVDF membranes while providing excellent temperature control.

The TE62 is designed to accept up to four cassettes simultaneously with cooling. Each cassette holds a 15 x 21 cm gel, or as many as four 7 x 10 cm mini gels.

• Uniform and strong electric field—supports efficient and even transfers
• Color-coded, easy to assemble cassettes - ensures proper orientation during transfer
• Superior tank design allows the cassettes to apply equal pressure across the stack - prevents gel distortion
• Built-in ceramic heat exchanger - provides excellent temperature control when used with an external cooling water bath  

Hoefer® Standard Transfer Tank with Cooling Chamber Includes

• Lower Chamber with Heat Exchanger, 1/ea
• Safety Lid with High Voltage Leads, 1/ea
• Electrode Panels, 2/pk
• Cassettes, 4/pk
• Foam Sponges, 6 mm thick, 4/pk
• Foam Sponges, 3 mm thick, 8/pk
• Cassette Hook, 1/ea
• Blotter Paper (14.5 x 21.5 cm), 25 sheets/pk 

Specifications
 

Capacity	Up to four 15 x 21 cm gels Up to sixteen 7 x 10 cm gels
Max. Power	100 V, 2 A, 200 W
Max. Temp.	113ºF | 45°C
Indoor Use	39.2 to 104ºF | 4 to 40°C
Humidity	Up to 80%
Dims (D x W x H)	12.60 x 11.02 x 6.50 In. | 32 x 28 x 16.5 cm

Gel Crusher™ Spin Column

• Creates uniform gel fragments with no chunks
• Fits in standard microtubes
• Increases DNA yield
• For use in molecular cloning, PCR, restriction digestions, sequencing, and labeling protocols
• Fits 2 mL receiver tubes 

MTC Bio’s Gel Crusher™ Spin Columns are designed to replace labor-intensive gel fragmentation steps (crush+soak method, etc.). Commonly used in molecular cloning, PCR, restriction digestions, sequencing, and labeling protocols, these spin columns can be used to fragment gel slices (typically cut with a gel extractor or razor blade) into smaller pieces.

These smaller uniform pieces will produce greater yields and can be easily pipetted using wide bore tips into an NaCl solution. This reduces the time to create a fine slurry for further elution, filtration, incubation, etc. Place the Gel Crusher™ column in the 2 mL microtube and centrifuge the column/tube assembly in a standard benchtop microcentrifuge for 5 minutes at maximum speed (generally approximately 15,000 x g).

The gel will be forced through the 600 uM holes into the 2 mL receiver tube. The crushed fragments will be small enough to be aspirated/dispensed through a pipette tip.

Reversible Racks

• Alphanumeric markings and are supplied in packs of 5.
• Choose 60 well (5 x 12) or 96 well (8 x 12) formats
• Reversible racks, accept 0.5 and 1.5 to 2.0 mL microtubes
• Alphanumeric marking on each well
• Made of polypropylene   

Azure HRP Stripping Buffer

Works by removing the primary and secondary antibodies from the membrane

Stripping buffer for stripping and re-probing chemiluminescent Western blots.

• Provided as a 1x ready-to-use solution
• Size: 500mL

Azure Aqua Transfer Cell

Perform a quick 1 hour transfer or transfer overnight at a lower voltage

Aqua Transfer Cell is used for transferring two mini gels to membranes for Western blotting experiments (wet transfer). The transfer cassettes and electrode core are colored for directionality which makes transferring easy and straightforward.

Includes:

• 2 transfer cassettes
• 6 sponges
• 2 Azure Aqua cooling units
• Tank and upper lid with power cables
• Transfer Electrode Cores

Features

The transfer cassettes and electrode core are colored for directionality, making transferring easy and straightforward.

Chemical reagent compatibility

Keep all components of the Azure Aqua Transfer Cell away from chloroform, arenes (such as methylbenzene benzene), and acetone. Any damage caused by these reagents is not covered by the warranty policy.

 
Cleaning Instructions

Before using, wash all components with a neutral detergent and rinse well with ultra pure water.

Wash the electrode assembly, transfer cassettes, and buffer tank with a neutral detergent and warm water. Be careful to avoid damaging the platinum electrodes. Use warm water to rinse the sponges, and then rinse all components with distilled or ultra-pure water.

Specifications
 

Azure Transfer Buffer

This proprietary transfer buffer provides improved detection of low-abundance and post-translationally modified proteins.

High-efficiency protein transfer and increased protein retention on the membrane add up to more sensitive Western blots.

• Provided as a 50x concentrated solution. Sufficient for 50-100 blots
• Size: 500mL

Features

• High performance—increased protein retention on the membrane and fast transfer times result in more sensitive detection of low-abundance and post-translationally modified proteins
• Efficient—enhanced transfer efficiency for proteins of all sizes
• Fast—transfer proteins in less than 20 minutes vs. several hours required with traditional transfer buffers
• Convenient—use your existing wet transfer electrophoresis apparatus

Azure ChemiWriter ECL Pen

Chemiluminescent pen for blot annotation

The Azure ChemiWriter ECL Western blot annotation pen is a chemiluminescent marker that enables you to write or draw on your transfer membranes, resulting in a chemiluminescent signal on your markings once substrate is added. Contains a reagent that reacts with HRP substrates to produce chemiluminescence and also a blue dye to allow you to see markings as you write on the Western blot.

Use for tracing visible protein ladders or making identifying marks on your Western blots. Pen has two tips on each end: one fine-tipped for thin markings and one thicker for depositing more of the reagent.

Quantity: 1 pen

Features

Enables you to write or draw on your transfer membranes. A chemiluminescent signal will be detectable from your markings once chemiluminescent substrate is added.

• Use for tracing visible protein ladders or making identifying marks on your blots. Pen has two tips on each end: one fine-tipped for thin markings and one thicker for depositing more of the reagent.
• There are two heads to the pen, a fine point tip and a thicker chisel point tip.
• We recommend using the fine point tip to annotate the blot and mark protein ladders for detection.
• The chisel point of the pen can be used to deposit an increased amount of reagent on the blot for more intense signal.
• For best results we recommend annotating your blot after transfer and before starting your Western blotting protocol.
• Once annotated, the blot may be stored dry or wet, refrigerated or at room temperature.
• Storage: pen should be stored tightly closed at room temperature. ChemiWriter ECL reagent and blue ink markings on the blot are stable for at least three months.