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ActionsResearch Essentials 2024 Promo!
For a limited time, buy 2 get 1 free OptiWell™ Deep Well Plates.
Must be the same items only. Enter Item # plus “-VF” for free product.
See flyer for details.
All promotions are valid while supplies last. Prices are subject to change without notice.
OptiWell™ Deep Well Plates - 384
Available in over 25 different types, The OptiWell™ line of Deep Well Plates is compatible with all popular automated research platforms and robotic equipment. These plates are useful for bacterial cultures, centrifugation (4000xg max RCF), and general sample storage. They can be used with silicone sealing mats and various types of sealing film. All plates are produced in a class 100,000 cleanroom facility and are ANSI / SLAS 1-2004 compliant. Although all OptiWell™ plates are autoclavable, several types are also available presterilized by gamma irradiation in accordance with ISO 13485. All plates are certified RNase/DNase free and non-pyrogenic.
- 24-Well, 48-well, 96-well, and 384-well configurations available
- For automated research platforms / robotic equipment
- Minimal binding/liquid retention
- Black printed alpha-numerics
- Virgin, medical-grade polypropylene
- Conical V-Bottom configurations
Azure HRP Stripping Buffer
Works by removing the primary and secondary antibodies from the membrane
Stripping buffer for stripping and re-probing chemiluminescent Western blots.
- Provided as a 1x ready-to-use solution
- Size: 500mL
Azure Aqua Transfer Cell
Perform a quick 1 hour transfer or transfer overnight at a lower voltage
Aqua Transfer Cell is used for transferring two mini gels to membranes for Western blotting experiments (wet transfer). The transfer cassettes and electrode core are colored for directionality which makes transferring easy and straightforward.
Includes:
- 2 transfer cassettes
- 6 sponges
- 2 Azure Aqua cooling units
- Tank and upper lid with power cables
- Transfer Electrode Cores
Features
The transfer cassettes and electrode core are colored for directionality, making transferring easy and straightforward.
Chemical reagent compatibility
Keep all components of the Azure Aqua Transfer Cell away from chloroform, arenes (such as methylbenzene benzene), and acetone. Any damage caused by these reagents is not covered by the warranty policy.
Cleaning Instructions
Before using, wash all components with a neutral detergent and rinse well with ultra pure water.
Wash the electrode assembly, transfer cassettes, and buffer tank with a neutral detergent and warm water. Be careful to avoid damaging the platinum electrodes. Use warm water to rinse the sponges, and then rinse all components with distilled or ultra-pure water.
Specifications
Parameters | Azure Aqua Transfer Cell Specs |
Number of cassettes | 2 |
Buffer requirement | 1100 mL |
Electrode distance | 4 cm |
Electrode assembly size ( L x W x H) | 17.5 cm x 13.5 cm x 19 cm |
Transfer time | 60 minutes |
Cooling | Azure Aqua cooling unit |
Blotting area | 7.5 cm x 10 cm |
Buffer volume (with cooling unit) | 650 mL |
Buffer volume (without cooling unit) | 850 mL |
Unit dimensions (W x L x H) | 13.5 cm x 17.5 cm x 19 cm |
Tank buffer volume | 1100 mL |
Maximum gel size | 7.5 cm x 10 cm |
Gel capacity | 2 precast or handcast gels |
Weight | 1.8 lbs |
Recommended power supply | Azure Aqua Power Supply 600V |
Azure Transfer Buffer
This proprietary transfer buffer provides improved detection of low-abundance and post-translationally modified proteins.
High-efficiency protein transfer and increased protein retention on the membrane add up to more sensitive Western blots.
- Provided as a 50x concentrated solution. Sufficient for 50-100 blots
- Size: 500mL
Features
- High performance—increased protein retention on the membrane and fast transfer times result in more sensitive detection of low-abundance and post-translationally modified proteins
- Efficient—enhanced transfer efficiency for proteins of all sizes
- Fast—transfer proteins in less than 20 minutes vs. several hours required with traditional transfer buffers
- Convenient—use your existing wet transfer electrophoresis apparatus
Azure ChemiWriter ECL Pen
Chemiluminescent pen for blot annotation
The Azure ChemiWriter ECL Western blot annotation pen is a chemiluminescent marker that enables you to write or draw on your transfer membranes, resulting in a chemiluminescent signal on your markings once substrate is added. Contains a reagent that reacts with HRP substrates to produce chemiluminescence and also a blue dye to allow you to see markings as you write on the Western blot.
Use for tracing visible protein ladders or making identifying marks on your Western blots. Pen has two tips on each end: one fine-tipped for thin markings and one thicker for depositing more of the reagent.
Quantity: 1 pen
Features
Enables you to write or draw on your transfer membranes. A chemiluminescent signal will be detectable from your markings once chemiluminescent substrate is added.
- Use for tracing visible protein ladders or making identifying marks on your blots. Pen has two tips on each end: one fine-tipped for thin markings and one thicker for depositing more of the reagent.
- There are two heads to the pen, a fine point tip and a thicker chisel point tip.
- We recommend using the fine point tip to annotate the blot and mark protein ladders for detection.
- The chisel point of the pen can be used to deposit an increased amount of reagent on the blot for more intense signal.
- For best results we recommend annotating your blot after transfer and before starting your Western blotting protocol.
- Once annotated, the blot may be stored dry or wet, refrigerated or at room temperature.
- Storage: pen should be stored tightly closed at room temperature. ChemiWriter ECL reagent and blue ink markings on the blot are stable for at least three months.
Azure Chemi Blot Blocking Buffer
This Chemi Blot Blocking Buffer was formulated to enhance signal and lower background by improving sensitivity. Enhances specific antibody-antigen interactions specifically for chemiluminescent Western blots.
- Provided as a 1x ready-to-use solution- no mixing or dilutions required.
- Size: 500mL
Features
Western blot membranes should be incubated in blocking buffer before antibody hybridization to prevent non-specific binding of antibodies. Blocking buffer can also be used for diluting primary and secondary antibodies during hybridization.
Azure Aqua Power Supply
Designed for powering electrophoresis and Western blotting transfer modules
This power supply can create and save protocols for all your gel and blotting experiments. Designed with you in mind, features a pause/resume function for flexibility.
Power supply includes output range of 5–600V, 1–1200mA, 1–500W, LCD readable voltage, current, power, and four power connections to run multiple experiments at once. Able to control constant power, current, or voltage.
Features
The Azure Aqua Power Supply is a universal power supply that is designed for powering electrophoresis and transfer modules. The power supply can create and save protocols for all your gel and blotting experiments and includes a pause/resume function for flexibility.
Azure Power Supply includes an output range of 5-600V, 1-1200mA, 1-500W, LCD readable voltage, current, power, and 4 connections for electrophoresis and transfer.
Specifications
Parameters | Azure Aqua Power Supply 600V Specs |
Output specifications | 600V, 1.2 A, 500W |
Output range (programmable) | 10-600V 10-1200mA1-500W |
Type of output | Constant voltage, current, and power |
Timer control | 1 min – 99 hr 59 min |
Pause/resume function | Yes |
Output terminals | 4 connections |
Unit dimensions (W x L x H) | 24 x 30.5 x 10 cm |
Weight | 5.6 lbs |
Stackable | Yes |
Regulatory | CE |
Safety features | Automatic monitoring of over-voltage, no load, sudden change of load, overload, and short circuit |
Azure Blot Washing Buffer
Wash buffers are an important part of the blotting process for removing unhybridized antibody and preventing background signal.
Blot washing buffer for use with Azure Imaging Systems, or any other Western blotting system.
- Provided as a 10x concentrated solution
- Size: 500mL
NOTE: While this Blot Washing Buffer is compatible with NIR Fluorescent Western blots, for the best results with NIR fluorescence, we recommend the Azure Fluorescent Blot Washing Buffer.
Azure Protein-free Blot Blocking Buffer
Protein-free blocking solution optimized for fluorescent Immunoblots and Western blots
Does not contain proteins. Azure Protein-Free Blot Blocking Buffer can reduce background in Western blots where the primary antibodies tend to cross-react with protein blockers like BSA, casein or milk protein. For low-quality primary antibodies that may require protein-based blocking agents, BSA or non-fat dry milk can be dissolved directly in the Azure Protein-Free Blot Blocking Buffer and then used to dilute the antibody.
Best used with assays where animal-based product usage is prohibited. Serves as an excellent blocking reagent for chemiluminescent Western blotting.
- Provided as a 5x solution
- Size: 200mL
Azure Western Blot Quenching Sheets
Background quenching sheets for use with fluorescent or chemiluminescent samples
Use these quenching sheets under your blot when imaging fluorescent or chemiluminescent Western blots. Sheets reduce background autofluorescence or background signal from contamination in shared imagers, improving the quality of your image.
- For use with fluorescent or chemiluminescent samples.
- Size: 9 x 12.5 cm
- Quantity: 10 Sheets
Features
Use these quenching sheets under your blot when imaging fluorescent or chemiluminescent Western blots.
The sheets reduce background autofluorescence or background signal from contamination in shared imagers, improving the overall quality of your image.
Azure Aqua Quad Mini-Cell
Vertical Gel Running System
Designed for running 1–4 precast or hand-cast gels (cassette size 10cm x 8cm) for electrophoresis. The locking side fasteners provide a tight seal that ensures rapid and easy electrophoresis. The unit comes equipped with 4 gel casting stands and casting frames, perfect for hand casting your own gels.
Simply align the spacer and short glass plates, seal between the hinges, and allow your gel to polymerize. Insert the polymerized gel into the electrophoresis module for an efficient gel-running experiment.
Includes:
- 4 gel casting stands and casting frames buffer dam
- 5 spacer plates (1mm)
- 10 short glass plates
- 5 15-well combs
- 5 10-well combs
- 5 sealing gaskets
- 5 gel shovels
- Tank and lid with power cables
- Primary and Secondary Electrode Cores
Features
- The Azure Aqua Quad Mini-Cell is designed for running 1–4 precast or hand-cast gels (cassette size 10cm x 8cm) for electrophoresis. The locking side fasteners provide a tight seal that ensures rapid and easy electrophoresis.
- The unit comes equipped with 4 gel casting stands and casting frames, perfect for hand casting your own gels: Simply align the spacer and short glass plates, seal between the hinges, and allow your gel to polymerize.
- Insert the polymerized gel into the electrophoresis module for an efficient gel-running experiment.
Specifications
Parameters | Azure Aqua Quad Mini-Cell Specs |
Unit dimensions (W x L x H) | 13.5 cm x 17.5 cm x 19 cm |
Weight | 2.2 lbs |
Recommended power supply | Azure Aqua Power Supply 600V |
Number of gels | 1-4 precast (most major brands) or handcast gels |
Tank buffer volume | 2 gels: 540 mL | 4 gels: 1100 mL |
Maximum gel size | 6.7 cm x 8.6 cm |
Typical run times | 30-45 minutes at 200V |
Gel cassette size (WxL) | 10 cm x 8 cm |
Azure Nitrocellulose Membranes
Pre-cut Nitrocellulose membrane for Western blotting
Low-fluorescence Nitrocellulose membranes are available in both 0.45 μm and 0.22 μm pore sizes to accommodate larger and smaller proteins, respectively. We recommend using the .45 µm pore size for the most analytical blotting: protein, ssDNA, and RNA transfers.
- Low molecular weight proteins
- Typically used for chemiluminescence
- Not ideal for stripping
- Excellent binding properties
Quantity: 10 membranes each