Azure Nitrocellulose Membranes

Pre-cut Nitrocellulose membrane for Western blotting

Low-fluorescence Nitrocellulose membranes are available in both 0.45 μm and 0.22 μm pore sizes to accommodate larger and smaller proteins, respectively. We recommend using the .45 µm pore size for the most analytical blotting: protein, ssDNA, and RNA transfers.

• Low molecular weight proteins
• Typically used for chemiluminescence
• Not ideal for stripping
• Excellent binding properties

Quantity: 10 membranes each   
 
 
 

Azure PVDF Membranes

Pre-cut PVDF membranes for Western blotting applications

Ideal for chemiluminescent and colorimetric Western blotting. Pre-cut to save you time in the lab. These low-fluorescence PVDF membranes reduce background noise and nonspecific protein binding for improved sensitivity. Available in three different pre-cut sizes.

Quantity: 10 membranes per package

Features

Low-fluorescence PVDF membranes reduce background noise for improved sensitivity. PVDF (polyvinylidene difluoride) membranes are commonly used for immunoblotting techniques, like Western blots. During the blotting process, samples are transferred from the protein gel onto a PVDF membrane by electroblotting. From there they can be used for protein sequencing.

• High binding capacity is great for low-expressing proteins
• Typically used for fluorescence
• High protein retention for stripping and reprobing

Azure Blotting Paper for Western Blotting

Pre-cut blotting paper for Western blot transfer

Forget about measuring and cutting blotting paper to the size of your mini-gels. Use these pre-cut papers to assemble your transfer “sandwiches”. Pre-cut blotting papers save time and reduce waste for the easiest Western blotting workflow.

Qty: 60 sheets

Blotting papers are cut to completely cover the gels and membranes.

For 8x10cm gels: blotting papers available in 9x13cm

For 7x9cm gels: blotting papers available in 8x10cm

Features

Pre-cut blotting papers save time and reduce waste for the easiest Western blotting workflow. Forget about measuring and cutting blotting paper to the size of your mini-gels! Use these pre-cut papers to assemble your transfer “sandwiches”.

Blotting papers come pre-cut to completely cover the gels and membranes.   
 
 
 

Hoefer® Semi-Dry Transfer Units

Hoefer® Semi-Dry Transfer Units are efficiently designed to use minimal buffer to transfer proteins from polyacrylamide gels onto membranes (either nitrocellulose or PVDF) in less than an hour. They support as many as two layers of gels being transferred simultaneously. The TE70XP has a 14 x 16 cm maximum transfer area, which can handle up to four mini gels using the stacked format. The TE77X and TE77XP have a 21 x 26 cm transfer area for a capacity of up to 12 mini gels at one time. 

• Included safety circuit breaker—limits voltage and current from the user's power supply, preventing electrical damage to the transfer unit
• Requires minimal current—does not generate the excessive heat that can dry out the transfer stack and halt transfer or damage transfer units 
• Durable iridium oxide and stainless steel electrodes—allow for contamination free, consistent transfer
• Vented electrodes—prevent buildup of bubbles which may impair transfer
• Minimal buffer requirements—reagent cost and preparation time are reduced
• The TE70XP and TE77XP Semi-Dry Transfer Units have an intelligent built-in power supply that prevents the stack from overheating by monitoring the transfer status 

All units include

• Molded Base with Platinum-Coated Titanium Anode and High Voltage Leads
• Hinged Lid with Stainless Steel Cathode  

TE77X includes

• Blotting Paper (21 x 26 cm), 25/pk
• Porous Cellophane (20 x 35.5 cm), 50/pk
• Mylar® Masks (23 x 27.5 cm), 2/pk
• Hinged Lid with Stainless Steel Cathode, 1/ea

TE70XP includes

• Blotting Paper (14 x 16 cm), 25/pk
• Porous Cellophane (20 x 35.5 cm), 50/pk
• Mylar® Masks (16.5 x 18.5 cm), 2/pk 

TE77XP includes

• Blotting Paper (21 x 26 cm), 25/pk
• Porous Cellophane (20 x 35.5 cm), 50/pk
• Mylar® Masks (23 x 27.5 cm), 2/pk

Specifications
 

Item #	TE77X	TE70XP	TE77XP
Transfer Area	Up to 21 x 26 cm Up to 8.27 x 10.24 In.	Up to 14 x 16 cm Up to 5.51 x 3.60 In.	Up to 21 x 26 cm Up to 8.27 x 10.24 In.
Max. Power	30 V, 500 mA, 15 W
Max. Temp.	113ºF | 45°C
Indoor Use	39.2 to 104ºF | 4 to 40°C
Humidity	Up to 80%
Dims (D x W x H)	3.54 x 14.96 x 18.11 In. | 9 x 38 x 46 cm
Weight	16.31 lb | 7.40 kg

Azure Flash Western Chemiluminescence Kit

Kit includes all reagents necessary for performing ten chemiluminescent Western blots using the Flash Western protocol

Each kit includes:

• 10x PVDF Membranes
• 100mL Chemi Blot Blocking Buffer
• 500μL Flash HRP Conjugate
• 250mL Flash Wash Buffer
• 100mL Radiance ECL Substrate

Features

Time savings using Azure Flash Western Chemiluminescent Kit. Flash Western graph includes use of Azure Transfer Buffer in light blue.

The Azure Flash Western Kit uses a proprietary set of reagents to achieve complete membrane blocking, rapid hybridization of antibodies, and enhancement of chemi signal in less than an hour of blotting time. The included transfer buffer cuts wet transfer time down to 15 to 30 minutes for a total assay time of two hours from gel to imaging.

• Fast protocol with few hands-on steps
• Antibody stabilization for enhanced chemi signal
• Low background and increased signal-to-noise ratios
• Increased detection sensitivity and linear dynamic range

Radiance ECL Chemiluminescent Substrate

Chemiluminescent HRP substrate for chemiluminescent Western blots

Radiance ECL is an HRP substrate with long-lasting signal and sensitivity down to low picograms. Use up to 10 times less antibody than other substrates used in life science.

Size: 500 mL

Features

• Performance — compare performance to SuperSignal West Pico and Amersham ECL
• Sensitivity — high sensitivity in low picograms
• Duration — Signal duration 6-8 hours
• Chemiluminescent Western Blotting
• The most common method for Western blot detection is chemiluminescence. Detection by chemiluminescence is relatively sensitive and works well for detecting individual or low abundance proteins.   

Radiance Plus Chemiluminescent Substrate

The most sensitive HRP substrate available for chemiluminescent Western blotting.

With attomole sensitivity and a long-lasting signal, Radiance Plus allows you to detect bands not visualized with other substrates. High signal-to-noise and a large dynamic range make it ideal for quantifying low-intensity bands.

Size: 150 mL

Features

• Low-abundance protein detection
• Compare performance to competitor products, like SuperSignal West Femto and Amersham ECL Select
• Sensitivity in low femtograms
• Signal duration 6-8 hours   

Radiance Q Chemiluminescent Substrate

Radiance Q chemiluminescent substrate is specially formulated for use with Azure Imaging Systems, or other CCD imagers. This novel horseradish peroxidase (HRP) substrate provides a strong, long-lasting signal, the broadest useful linear range, and high sensitivity for the most quantitative chemiluminescent Western assays.

Size: 150 mL

Features
 

Increase the sensitivity and quantitative accuracy of your chemiluminescent Western blots. Specially developed for CCD imaging, Radiance Q produces a strong, long-lasting signal with extremely low background, perfect for detecting low abundance proteins. Because the substrate is not depleted at high protein loads, Radiance provides the largest dynamic range of any chemiluminescent substrate, improving quantitative accuracy.

• Extended dynamic range for detecting low and high abundance proteins within the same blot
• Compare performance to SuperSignal West Dura and Amersham ECL Prime
• Sensitivity in mid femtograms
• Signal duration 10-24 hours   

PurePlus® Deep Well Plates

Labcon’s PurePlus® Deep Well Plates are rigorously tested to ensure they are free of RNase, DNase, ATP, and endotoxins. With our in-house microbiology lab, consistency is a top priority.

Available in sterile and non-sterile formats, these plates fit most popular instruments and can be sealed with sealing films or reusable silicone mats. Barcoding is available for both 1.2 mL and 2.2 mL plates by special order.

Ultra Clean & Ultra Clear 

PurePlus® plates feature molded-in orientation and permanent printed locating grids for ease of use. They are available in multiple formats: 96 well square hole with conical or flat bottom, 96 well round hole with round bottom, and 384 well square hole.

With volumes ranging from 0.2 mL to 2.5 mL, our plates are offered in non-sterile and validated medical-grade sterile versions, sterilized to an SAL of 10^-6 in compliance with ISO 11137.

The 1.2 mL and 2.2 mL volumes include three-sided alpha-numeric labeling grids, and their smooth interior surfaces ensure efficient pipetting. Designed for manual or robotic handling, these plates are SBS formatted for compatibility with automated systems.

Additional Features

Made from 100% virgin polypropylene, PurePlus® plates are autoclavable and freezable. Unlike "pre-sterile" products from other manufacturers, our sterile plates are fully validated for diagnostic and regulated procedures.

Specifications

For a limited time, enjoy discounted pricing on select Hoefer® Mighty Small Precast Gels!

(MSG010T, MSG012T, MSG412T, or MSG420T) 
  

Don't miss out - Stock up before this offer ends!
   

Promo valid from 6/10/25 to 6/30/25. Discount applies to the listed price.
Offer cannot be combined with other promotions, discounts, or institutional pricing. Not valid on prior purchases.
Special price applies only to the specified item; no substitutions.

Allterms and conditionsapply.

Hoefer® Mighty Small precast gels are high-performance mini polyacrylamide gels commonly used for protein separation. Utilizing the Bis-Tris buffer system, they effectively minimize protein modifications during electrophoresis, resulting in sharper and clearer protein bands with higher resolution.

• High loading capacity: 50 uL max. sample volume
• High resolution: Upgraded bis-Tris system for clearer, sharper separation bands and higher resolution
• Ready to use and easy to store: No solution preparation or gel casting is required, saving time. Store at 2 to 8ºC
• Good Repeatability: Fully automated gel casting process, minimal batch-to-batch variation, high stability, and good repeatability
• Highly effective: Max 40 min for electrophoresis
• Safe and non-toxic: No hazardous reagents need to be handled 

Compatible with Hoefer® Mighty Small II Vertical Electrophoresis Systems and the Hoefer® miniVE Vertical Protein Electrophoresis and Blotting Systems.  

Specifications
 

Item #	MSGT	MSGF
Plate Size	3.94 x 3.15 In. | 10 x 8 cm
Gel Size	3.35 x 2.76 In. | 8.5 x 7 cm, 1mm thickness
System/Buffer	Bis-Tris System, MOPS-SDS Buffer	Bis-Tris System, MOPS Buffer
Wells	12-well, 15-well
Storage	Store at 2 to 8°C. (35.6 to 46.4ºF) Do not freeze.
Concentration	Gradient Gels: 4 to 12%, 4 to 20% | Homogenous Gels: 10%, 12%
Loading Vol.	For 12 wells: max 50 uL (recommended: ≤ 25 uL) | For 15 wells: max 30 uL (recommended: ≤ 15 uL)

Multi-colored 96 Well Orienter

For easy identification of samples in microplates, vertically or horizontally oriented bright colors make it easy to distinguish between columns. Fits most standard flat and round bottom 96-well plates. Available in horizontal and vertical orientations.
 

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Glow-Writer Pen

Fine-Point Phosphorescent Marking Pen for Laboratory Autography

• Fine Point Nib- Provides fine writing and greater information density
• Specially Designed- Ideal glow intensity and afterglow duration helps prevent over-andunder exposure
• No Ink Clogs- Low ink viscosity and porous nib prevents ink from clogging