Radiance ECL Chemiluminescent Substrate

Chemiluminescent HRP substrate for chemiluminescent Western blots

Radiance ECL is an HRP substrate with long-lasting signal and sensitivity down to low picograms. Use up to 10 times less antibody than other substrates used in life science.

Size: 500 mL

Features

• Performance — compare performance to SuperSignal West Pico and Amersham ECL
• Sensitivity — high sensitivity in low picograms
• Duration — Signal duration 6-8 hours
• Chemiluminescent Western Blotting
• The most common method for Western blot detection is chemiluminescence. Detection by chemiluminescence is relatively sensitive and works well for detecting individual or low abundance proteins.   

Radiance Plus Chemiluminescent Substrate

The most sensitive HRP substrate available for chemiluminescent Western blotting.

With attomole sensitivity and a long-lasting signal, Radiance Plus allows you to detect bands not visualized with other substrates. High signal-to-noise and a large dynamic range make it ideal for quantifying low-intensity bands.

Size: 150 mL

Features

• Low-abundance protein detection
• Compare performance to competitor products, like SuperSignal West Femto and Amersham ECL Select
• Sensitivity in low femtograms
• Signal duration 6-8 hours   

Radiance Q Chemiluminescent Substrate

Radiance Q chemiluminescent substrate is specially formulated for use with Azure Imaging Systems, or other CCD imagers. This novel horseradish peroxidase (HRP) substrate provides a strong, long-lasting signal, the broadest useful linear range, and high sensitivity for the most quantitative chemiluminescent Western assays.

Size: 150 mL

Features

Increase the sensitivity and quantitative accuracy of your chemiluminescent Western blots. Specially developed for CCD imaging, Radiance Q produces a strong, long-lasting signal with extremely low background, perfect for detecting low abundance proteins. Because the substrate is not depleted at high protein loads, Radiance provides the largest dynamic range of any chemiluminescent substrate, improving quantitative accuracy.

• Extended dynamic range for detecting low and high abundance proteins within the same blot
• Compare performance to SuperSignal West Dura and Amersham ECL Prime
• Sensitivity in mid femtograms
• Signal duration 10-24 hours   

PurePlus® Deep Well Plates

Labcon’s PurePlus® Deep Well Plates are rigorously tested to ensure they are free of RNase, DNase, ATP, and endotoxins. With our in-house microbiology lab, consistency is a top priority.

Available in sterile and non-sterile formats, these plates fit most popular instruments and can be sealed with sealing films or reusable silicone mats. Barcoding is available for both 1.2mL and 2.2mL plates by special order.

Ultra Clean & Ultra Clear

PurePlus® plates feature molded-in orientation and permanent printed locating grids for ease of use. They are available in multiple formats: 96 well square hole with conical or flat bottom, 96 well round hole with round bottom, and 384 well square hole.

With volumes ranging from 0.2mL to 2.5mL, our plates are offered in non-sterile and validated medical-grade sterile versions, sterilized to an SAL of 10^-6 in compliance with ISO 11137.

The 1.2mL and 2.2mL volumes include three-sided alpha-numeric labeling grids, and their smooth interior surfaces ensure efficient pipetting. Designed for manual or robotic handling, these plates are SBS formatted for compatibility with automated systems.

Additional Features

Made from 100% virgin polypropylene, PurePlus® plates are autoclavable and freezable. Unlike "pre-sterile" products from other manufacturers, our sterile plates are fully validated for diagnostic and regulated procedures.

Aluminum Autoradiography Cassette 5 x 7

Classic Push-Button Cassettes

• Constructed From Lightweight Aluminum & Nylon
• Special Curved Door Gives Exceptional Screen-Film Contact

RunBlue gels are more rigid and up to 10 times stronger than conventional precast gels resulting in more robust gels that can be handled with confidence. No tearing - an 8% gel can be thrown into the air and caught without breaking!

• RunBlue gels are manufactured using a unique method of polymerisation that results in more uniform and more complete polymerisation of the Acrylamide when compared to conventional, chemical based methods. This results in gels that have superior batch to batch reproducibility and practically eliminates exposure to un-polymerised Acrylamide.
• RunBlue gels incorporate a stacking gel that has been dyed (red for SDS) for the simplest ever sample loading. In addition, there is no comb to remove as the top of the teeth are fixed in place. This not only prevents the teeth from being accidentally torn off during comb removal but that all of the wells are uniform, with the teeth remaining vertical. This prevents sample cross contamination / inability to load desired Vol. The teeth also protrude above the lower front plate of the gel cassette preventing sample flow over into adjacent wells.
• Runblue gels are available in two different cassette sizes providing compatibility with the most common gel tanks and apparatus.  10x10 cm cassettes are compatible with Invitrogen/Life Technologies equipment and any other equipment designed for this format, such as the Hoeffer and Owl tanks. Our 8x10 cm cassette is designed to fit the Biorad equipment (Mini Protean I, II and II, Tetra etc).
• The resolving gel on RunBlue gels extends to the very bottom of the cassette which not only provides improved resolution but also there is no protruding gel that requires trimming prior to blotting. There is also no additional tape / covers to remove - simply take the gel from the pack and use.
• RunBlue gels are supplied in boxes of 10 gels. They are available in a 12 well format, covering a range of concentrations and gradients.

Benefits of RunBlue Precast Gels

• 2 year shelf life - never throw away another gel
• Up to 10x stronger - no more torn, ruined gels
• Load up to 20µl - see everything in your sample
• Dyed stacking gel - makes gel loading child's play (SDS = Red)
• No comb to remove - no more broken or bent teeth
• Full length resolving gel - improved resolution and better blotting
• 10x10 cm and 8x10 cm cassette - total compatibility with most popular tanks including Invitrogen and Biorad equipment
• Fast delivery with immediate and expert technical support for total peace of mind
• Best value gels available - save money!

MAXI Flex Tube Kit

MAXI Flex Tubes combine two modes of action: electro-elution of nucleic acid molecules from polyacrylamide or agarose gels and dialysis or buffer exchange of protein molecules. Flex Tubes allow rapid, secure, simple loading and recovery, with high performance as the most convenient, user friendly, electro-elution and dialysis system on the market.

KIT CONTENTS

• Flex Tubes 2/10/30/50/ 100 pieces
• Supporting tray (for electro elution protocol) 1ea. (select kits)
• Floating rack (for dialysis protocol) 1ea. (select kits)
• Information and Protocol Manual 1ea.

SPECIFICATIONS

• Membrane cut-off: 3.5K(11bp), 6-8K(18-24bp), 12-14K(36-42bp), 25K(76bp) or 50K(152bp) MWCO
• Tube volume: 3ml
• Dialysis volume: 0.1-3ml
• Min. sample size for extraction: 20µg
• Max. gel slice: 2cm x 1cm
• Membrane ultra-clean, sulfur and heavy metal free. EDTA treated
• Flex Tube MWCO are in kilo Daltons (K) for proteins and corresponding base pairs (bp) for nucleic acids as indicated in the table below:

APPLICATIONS

• Dialysis, electro-elution or buffer exchange with volumes between 0.1-3ml
• Preparation of protein samples for MALDI-MS
• Sample concentration
• Large-scale protein dialysis, such as antibodies and recombinant protein purification
• Removal of contaminating micro-molecules
• Tissue culture extraction purification
• Removal of salts, surfactants, solvents, and detergents
• Complex formation studies (protein-protein, protein-DNA, and protein-RNA)
• pH and buffer adjustment of sample solutions, protein extraction or cell extraction
• High throughput dialysis
• Peptide dialysis, as small as 10 amino acids
• Virus-particles purification

MIDI Flex Tube Kit

MIDI Flex Tubes combine two modes of action: electro-elution of nucleic acid molecules from polyacrylamide or agarose gels and dialysis or buffer exchange of protein molecules. Flex Tubes allow rapid, secure, simple loading and recovery, with high performance as the most convenient, user friendly, electro-elution and dialysis system on the market.

KIT CONTENTS

• Flex Tubes 2/10/30/50/ 100 pieces
• Supporting tray (for electro elution protocol) 1ea. (select kits)
• Floating rack (for dialysis protocol) 1ea. (select kits)
• Information and Protocol Manual 1ea.

SPECIFICATIONS

• Membrane cut-off: 1K(3bp), 3.5K(11bp) or 6-8K(18-24bp) MWCO
• Tube volume: 800µl
• Dialysis volume: 50-800µl
• Min. sample size for extraction: 0.5µg
• Max. gel slice: 1cm x 0.5cm
• Membrane ultra-clean, sulfur and heavy metal free. EDTA treated
• Flex Tube MWCO are in kilo Daltons (K) for proteins and corresponding base pairs (bp) for nucleic acids as indicated in the table below:

APPLICATIONS

• Dialysis, electro-elution or buffer exchange with volumes between 50-800µl
• Preparation of protein samples for MALDI-MS
• Sample concentration
• Large-scale protein dialysis, such as antibodies and recombinant protein purification
• Removal of contaminating micro-molecules
• Tissue culture extraction purification
• Removal of salts, surfactants, solvents, and detergents
• Complex formation studies (protein-protein, protein-DNA, and protein-RNA)
• pH and buffer adjustment of sample solutions, protein extraction or cell extraction
• High throughput dialysis
• Peptide dialysis, as small as 10 amino acids
• Virus-particles purification.

MEGA Flex Tube Kit

MEGA Flex Tubes enable user friendly dialysis or buffer exchange of protein molecules with rapid, secure and simple loading along with high performance yield recovery.

KIT CONTENTS

• Flex Tubes 2/10/30/50/ 100 pieces
• Floating rack (for dialysis protocol) 1ea. (select kits)
• Information and Protocol Manual 1ea.

SPECIFICATIONS

• Membrane cut-off: 1K, 3.5K, 6-8K or 12-14K MWCO
• Tube volume: 10ml, 15ml or 20ml
• Dialysis volume: 3-20ml
• Membrane ultra-clean, sulfur and heavy metal free. EDTA treated
• Flex Tube MWCO are in kilo Daltons (K) for proteins

APPLICATIONS

• Dialysis or buffer exchange with volumes between 3-20ml
• Preparation of protein samples for MALDI-MS
• Sample concentration
• Large-scale protein dialysis, such as antibodies and recombinant protein purification
• Removal of contaminating micro-molecules
• Tissue culture extraction purification
• Removal of salts, surfactants, solvents, and detergents
• Complex formation studies (protein-protein, protein-DNA, and protein-RNA)
• pH and buffer adjustment of sample solutions, protein extraction or cell extraction
• High throughput dialysis
• Peptide dialysis, as small as 10 amino acids
• Virus-particles purification

MINI Flex Tube Kit

MINI Flex Tubes combine two modes of action: electro-elution of nucleic acid molecules from polyacrylamide or agarose gels and dialysis or buffer exchange of protein molecules. Flex Tubes allow rapid, secure, simple loading and recovery, with high performance as the most convenient, user friendly, electro-elution and dialysis system on the market.

KIT CONTENTS

• Flex Tubes 2/10/30/50/ 100 pieces
• Supporting tray (for electro elution protocol) 1ea. (select kits)
• Floating rack (for dialysis protocol) 1ea. (select kits)
• Information and Protocol Manual 1ea.

SPECIFICATIONS

• Membrane cut-off: 6-8K(18-24bp), 12-14K(36-42bp) or 25K(76bp) MWCO
• Tube volume: 250µl
• Dialysis volume: 10-250µl
• Min. sample size for extraction: 0.5µg
• Max. gel slice: 0.4cm x 1.1cm
• Membrane ultra-clean, sulfur and heavy metal free. EDTA treated
• Flex Tube MWCO are in kilo Daltons (K) for proteins and corresponding base pairs (bp) for nucleic acids as indicated in the table below:

APPLICATIONS

• Dialysis, electro-elution or buffer exchange with volumes between 10-250µl
• Preparation of protein samples for MALDI-MS
• Sample concentration
• Large-scale protein dialysis, such as antibodies and recombinant protein purification
• Removal of contaminating micro-molecules
• Tissue culture extraction purification
• Removal of salts, surfactants, solvents, and detergents
• Complex formation studies (protein-protein, protein-DNA, and protein-RNA)
• pH and buffer adjustment of sample solutions, protein extraction or cell extraction
• High throughput dialysis
• Peptide dialysis, as small as 10 amino acids
• Virus-particles purification

Enduro 15 x 10 cm Horizontal Gel Rig

• Molded, durable construction
• Rubber gates for easy, tapeless gel casting
• Adjustable height combs
• Loading guides aid in well visualization

Enduro Horizontal Gel Boxes have been designed for ease of use, safety and durability. A variety of sizes are available, from a mini gel system to a large maxi gel system that can accommodate as many as 450 samples in a single run. A wide range of accessories are available to customize your system, including multichannel compatible gel combs.

QUALITY CONSTRUCTION - The Enduro gel tanks are molded for leakproof performance and a long service life. The corrosion resistant platinum electrodes are located in cassettes to protect them during cleaning and provide for easy replacement. The lids fit on the gel boxes in only one direction. A drip ring on the lid recycles condensed buffer and prevents it from dripping directly onto the gel.

EASE OF USE - Casting gels for the Enduro system is very easy. Simply snap the rubber gates onto the ends if the tray, place the comb in position and pour the gel - no taping is necessary. Slots on the sides of the gel trays hold the combs in place. The two piece combs are designed so that the teeth can be raised or lowered to vary the depth of the well within the gel. The supplied combs are 1.5 mm thick. Combs of other thicknesses are available separately. After the gel has hardened, the combs and gates are removed and the gel and tray placed in the gel box. Loading strips may be placed on the bottom of the gel box to aid in well visualization. Gel trays are UV transparent.

DESIGNED FOR SAFETY - The power leads for the Enduro system are attached to the gel box lid. The connection with the electrodes is made only when the lid is properly in place. Removal of the lid removes power from the system. Side tabs and pressure pads make lid removal easy and prevent sloshing of buffer.

Specifications 

Enduro 10 x 10 cm Horizontal Gel Rig

The Enduro 96 is an ideal complement to the Labnet Enduro Horizontal Gel Box line. The 96-well format unit is compatible with 8-channel pipettes and matches up with the standard 96 well plate configuration. The average run time is 15-30 minutes. The gel is in a 10 X 12 cm format with options for either 1 or 2 marker lanes. The layout of wells is designed to allow a run length of 1.8 cm per sample providing ideal separation. For those who already own an Enduro E1010, the Enduro 96 comb sets are available and compatible with these existing units.
Features include:

• 96 well format
• MCP compatible
• For high throughput analysis of PCR products

Specifications
 

Gel Dimensions	10 x 12 cm
Unit dimensions	22 x 12.5 x 9 cm
Buffer Volume	300 mL
Max sample capacity	96 samples plus 1 or 2 markers
Combs/number of samples	8 + 1x marker; 8 x 2x marker
Thickness	1; 1.5 mm