Azure Protein-free Blot Blocking Buffer

Protein-free blocking solution optimized for fluorescent Immunoblots and Western blots

Does not contain proteins. Azure Protein-Free Blot Blocking Buffer can reduce background in Western blots where the primary antibodies tend to cross-react with protein blockers like BSA, casein or milk protein. For low-quality primary antibodies that may require protein-based blocking agents, BSA or non-fat dry milk can be dissolved directly in the Azure Protein-Free Blot Blocking Buffer and then used to dilute the antibody.

Best used with assays where animal-based product usage is prohibited. Serves as an excellent blocking reagent for chemiluminescent Western blotting.

• Provided as a 5x solution
• Size: 200mL

Azure Western Blot Quenching Sheets

Background quenching sheets for use with fluorescent or chemiluminescent samples

Use these quenching sheets under your blot when imaging fluorescent or chemiluminescent Western blots. Sheets reduce background autofluorescence or background signal from contamination in shared imagers, improving the quality of your image.

• For use with fluorescent or chemiluminescent samples.
• Size: 9 x 12.5 cm
• Quantity: 10 Sheets

Features

Use these quenching sheets under your blot when imaging fluorescent or chemiluminescent Western blots.

The sheets reduce background autofluorescence or background signal from contamination in shared imagers, improving the overall quality of your image.

Azure Aqua Quad Mini-Cell

Vertical Gel Running System

Designed for running 1–4 precast or hand-cast gels (cassette size 10cm x 8cm) for electrophoresis. The locking side fasteners provide a tight seal that ensures rapid and easy electrophoresis. The unit comes equipped with 4 gel casting stands and casting frames, perfect for hand casting your own gels.

Simply align the spacer and short glass plates, seal between the hinges, and allow your gel to polymerize. Insert the polymerized gel into the electrophoresis module for an efficient gel-running experiment.

Includes:

• 4 gel casting stands and casting frames buffer dam
• 5 spacer plates (1mm)
• 10 short glass plates
• 5 15-well combs
• 5 10-well combs
• 5 sealing gaskets
• 5 gel shovels
• Tank and lid with power cables
• Primary and Secondary Electrode Cores

Features

• The Azure Aqua Quad Mini-Cell is designed for running 1–4 precast or hand-cast gels (cassette size 10cm x 8cm) for electrophoresis. The locking side fasteners provide a tight seal that ensures rapid and easy electrophoresis.
• The unit comes equipped with 4 gel casting stands and casting frames, perfect for hand casting your own gels: Simply align the spacer and short glass plates, seal between the hinges, and allow your gel to polymerize.
• Insert the polymerized gel into the electrophoresis module for an efficient gel-running experiment.

Specifications
 

Parameters	Azure Aqua Quad Mini-Cell Specs
Unit dimensions (W x L x H)	13.5 cm x 17.5 cm x 19 cm
Weight	2.2 lbs
Recommended power supply	Azure Aqua Power Supply 600V
Number of gels	1-4 precast (most major brands) or handcast gels
Tank buffer volume	2 gels: 540 mL | 4 gels: 1100 mL
Maximum gel size	6.7 cm x 8.6 cm
Typical run times	30-45 minutes at 200V
Gel cassette size (WxL)	10 cm x 8 cm

 

Azure Nitrocellulose Membranes

Pre-cut Nitrocellulose membrane for Western blotting

Low-fluorescence Nitrocellulose membranes are available in both 0.45 μm and 0.22 μm pore sizes to accommodate larger and smaller proteins, respectively. We recommend using the .45 µm pore size for the most analytical blotting: protein, ssDNA, and RNA transfers.

• Low molecular weight proteins
• Typically used for chemiluminescence
• Not ideal for stripping
• Excellent binding properties

Quantity: 10 membranes each   
 
 
 

Azure PVDF Membranes

Pre-cut PVDF membranes for Western blotting applications

Ideal for chemiluminescent and colorimetric Western blotting. Pre-cut to save you time in the lab. These low-fluorescence PVDF membranes reduce background noise and nonspecific protein binding for improved sensitivity. Available in three different pre-cut sizes.

Quantity: 10 membranes per package

Features

Low-fluorescence PVDF membranes reduce background noise for improved sensitivity. PVDF (polyvinylidene difluoride) membranes are commonly used for immunoblotting techniques, like Western blots. During the blotting process, samples are transferred from the protein gel onto a PVDF membrane by electroblotting. From there they can be used for protein sequencing.

• High binding capacity is great for low-expressing proteins
• Typically used for fluorescence
• High protein retention for stripping and reprobing

Azure Blotting Paper for Western Blotting

Pre-cut blotting paper for Western blot transfer

Forget about measuring and cutting blotting paper to the size of your mini-gels. Use these pre-cut papers to assemble your transfer “sandwiches”. Pre-cut blotting papers save time and reduce waste for the easiest Western blotting workflow.

Qty: 60 sheets

Blotting papers are cut to completely cover the gels and membranes.

For 8x10cm gels: blotting papers available in 9x13cm

For 7x9cm gels: blotting papers available in 8x10cm

Features

Pre-cut blotting papers save time and reduce waste for the easiest Western blotting workflow. Forget about measuring and cutting blotting paper to the size of your mini-gels! Use these pre-cut papers to assemble your transfer “sandwiches”.

Blotting papers come pre-cut to completely cover the gels and membranes.   
 
 
 

Azure Flash Western Chemiluminescence Kit

Kit includes all reagents necessary for performing ten chemiluminescent Western blots using the Flash Western protocol

Each kit includes:

• 10x PVDF Membranes
• 100mL Chemi Blot Blocking Buffer
• 500μL Flash HRP Conjugate
• 250mL Flash Wash Buffer
• 100mL Radiance ECL Substrate

Features

Time savings using Azure Flash Western Chemiluminescent Kit. Flash Western graph includes use of Azure Transfer Buffer in light blue.

The Azure Flash Western Kit uses a proprietary set of reagents to achieve complete membrane blocking, rapid hybridization of antibodies, and enhancement of chemi signal in less than an hour of blotting time. The included transfer buffer cuts wet transfer time down to 15 to 30 minutes for a total assay time of two hours from gel to imaging.

• Fast protocol with few hands-on steps
• Antibody stabilization for enhanced chemi signal
• Low background and increased signal-to-noise ratios
• Increased detection sensitivity and linear dynamic range

Radiance ECL Chemiluminescent Substrate

Chemiluminescent HRP substrate for chemiluminescent Western blots

Radiance ECL is an HRP substrate with long-lasting signal and sensitivity down to low picograms. Use up to 10 times less antibody than other substrates used in life science.

Size: 500 mL

Features

• Performance — compare performance to SuperSignal West Pico and Amersham ECL
• Sensitivity — high sensitivity in low picograms
• Duration — Signal duration 6-8 hours
• Chemiluminescent Western Blotting
• The most common method for Western blot detection is chemiluminescence. Detection by chemiluminescence is relatively sensitive and works well for detecting individual or low abundance proteins.   

Radiance Plus Chemiluminescent Substrate

The most sensitive HRP substrate available for chemiluminescent Western blotting.

With attomole sensitivity and a long-lasting signal, Radiance Plus allows you to detect bands not visualized with other substrates. High signal-to-noise and a large dynamic range make it ideal for quantifying low-intensity bands.

Size: 150 mL

Features

• Low-abundance protein detection
• Compare performance to competitor products, like SuperSignal West Femto and Amersham ECL Select
• Sensitivity in low femtograms
• Signal duration 6-8 hours   

Radiance Q Chemiluminescent Substrate

Radiance Q chemiluminescent substrate is specially formulated for use with Azure Imaging Systems, or other CCD imagers. This novel horseradish peroxidase (HRP) substrate provides a strong, long-lasting signal, the broadest useful linear range, and high sensitivity for the most quantitative chemiluminescent Western assays.

Size: 150 mL

Features

Increase the sensitivity and quantitative accuracy of your chemiluminescent Western blots. Specially developed for CCD imaging, Radiance Q produces a strong, long-lasting signal with extremely low background, perfect for detecting low abundance proteins. Because the substrate is not depleted at high protein loads, Radiance provides the largest dynamic range of any chemiluminescent substrate, improving quantitative accuracy.

• Extended dynamic range for detecting low and high abundance proteins within the same blot
• Compare performance to SuperSignal West Dura and Amersham ECL Prime
• Sensitivity in mid femtograms
• Signal duration 10-24 hours   

LabNet LabValues Promotions 2024!

For a limited time enjoy discounted pricing on the ENDURO™ Power Supply Series (E0303 and E0203)

See flyer for more details. 

 

Promo is valid 07/1/24 – 12/31/24.

Not to be combined with any offers or discounts. No institutional pricing. All terms apply.   

ENDURO™ Power Supply 

• Two models (300V and 250V) available
• For DNA, RNA, or protein electrophoresis, and blotting applications
• Constant voltage or constant current with automatic crossover
• 250V model also offers a constant power operating mode
• Timed or continuous operation
• Short circuit and over current/ over voltage protection
• No load and load change detection system
• Programming includes option for automatic recovery after power failure
• 3 year warranty

The new Enduro Power Supplies meet a wide range of applications including DNA, RNA, and protein electrophoresis, blotting, and many others. Two models are available with voltage ranges to 300V and current ranges to 3000mA. Operating modes include constant voltage or constant current with automatic crossover. In addition, the 250V model offers a constant power operating mode.

All parameters, including voltage, current, power (250V model only), and time are easily set using the membrane keypad and displayed digitally on the back lit LCD. Compact in design with a small footprint, the Enduro Power Supplies are also stackable to save space. For easy viewing and setting of parameters on the bench or on a shelf, the front of the power supply can be raised with the integral folding feet.

Specifications

LabNet LabValues Promotions 2024!

 

For a limited time enjoy discounted pricing on select Labnet Enduro™ UV Transilluminators

 

See flyer for more details.

 

Promo is valid 07/1/24 – 12/31/24.

Not to be combined with any offers or discounts. No institutional pricing. All terms apply.

Enduro™ UV Transilluminators