• RNA to cDNA to qPCR, in one tube
• High purity enzyme formulation for enhanced stability and performance
• Accuris Hot-Start Taq allows for preparation at room-temperature
• Blue dye facilitates pipetting and visualization in plates
• Available for green fluorescence or probe detection
• Multiplex formulation available for multiple target amplification

Accuris E3200 UV Transilluminator

Accuris UV Transilluminators feature space-saving designs, to fit perfectly in any busy laboratory. Three models are available, with a range of intensity settings and viewing surface sizes. Choose your model depending on your application and the size or number of gels to analyze. 

The E3200 offers a large viewing surface of 21 x 26 cm to accommodate larger gels or multiple small gels. High powered, 14W UV bulbs provide higher sensitivity, so the smallest bands of stained nucleic acids can be visualized. The E3200 also features a control knob for continuous adjustment of the UV intensity.

All Accuris UV Transilluminators incorporate scratch-resistant, UV filter glass. Peak output emission of 302 nm is ideal for excitation of gels stained with Ethidium Bromide, SmartGlow, SYBR Green and other common gel stains.

Acrylic UV blocking covers allow safe viewing of samples, and friction hinges on the front edge allow positioning of the cover at an angle for gel access.      

Specifications
 

Item	E3200 UV Transilluminator
Light Source	4 x 14W UV Bulbs
Exterior Dimensions (W x D x H)	13.6 x 11.8 x 3.5 In.  | 34.5 x 30 x 9 cm
Electrical	24VDC (AC power adapter is included)
Output Wavelength	Peak at 302 nm
Viewing Surface	8.27 x 10.24 In. | 21 x 26 cm
Weight	5.15 lbs. | 2.3 kg.
Warranty	2 years

Accuris qMAX One-Step RT-qPCR kits

• RNA to cDNA to qPCR, in one tube
• High purity enzyme formulation for enhanced stability and performance
• Accuris Hot-Start Taq allows for preparation at room-temperature
• Blue dye facilitates pipetting and visualization in plates
• Available for green fluorescence or probe detection
• Multiplex formulation available for multiple target amplification
• Bulk pricing available for high throughput labs and kit manufacturers contact us for details

Accuris qMAX™ One Step Kits allow for highly sensitive real time RT-qPCR assays to be performed directly from RNA templates. Workflows are simplified with optimized formulations of ready-to-use 2X qPCR master mix and 20X reverse transcriptase.

Optimized buffer includes powerful RNase inhibitors, and an extremely thermostable MMLV-derived reverse transcriptase enables robust first strand cDNA synthesis. Accuris Hot Start Taq uses an antibody mediated hot start mechanism allowing for sample preparation at room temperature. Only after an initial incubation at 95C will the Taq become active, so non-specific amplification is greatly reduced. An inert blue dye is included in the Taq master mix to help simplify pipetting and reduce errors.

Three versions of our One Step qPCR kits are available:

qMAX Green One Step kits incorporate our proprietary intercalating dye which exhibits higher fluorescent and lower PCR inhibition than other popular dyes such as SYBR.

qMAX Probe One Step kits are optimized for use with popular TaqMan, Scorpions, and molecular beacon probes.

qMAX Probe One Step Multiplex kits are specifically developed and optimized for efficient probe-based detection of multiple targets in a single reaction well. The Multiplex formulation is comprised of a 20x reverse transcriptase and 2x PCR Mix preparation ideally suited for complex RNA samples including low-copy number viral RNA commonly used in the clinical and research laboratory. qMAX Probe One Step Multiplex kits have been designed to overcome the many challenges of multiplex RT-PCR, by addressing important factors such as the balance between magnesium chloride and deoxynucleotide concentrations, the relative Taq Polymerase and reverse transcriptase concentration, and the ionic conditions of the core reaction buffer.

All Accuris One-Step Kits are compatible with standard and fast cycling protocols and provide increased sensitivity, speed, and reproducibility for a broad range of samples and targets. The polymerase mix is available with different levels of ROX reference dye for compatibility with all qPCR instruments.

Accuris Fast Extraction PCR Kit

The Accuris Fast Extraction PCR Kit features an exclusive single-tube solution that extracts genomic DNA in just 8 minutes. This is swiftly followed by PCR amplification utilizing our High Fidelity Hot Start Red Dye Master Mix, designed for superior endpoint PCR performance. High Fidelity Hot Start Red Dye Master Mix is a 2X formula that incorporates a non-reactive red dye, enabling direct gel loading.

This kit offers versatility to suit different workflows: the PCR-prepared genomic DNA can be directly used in endpoint PCR or can be applied in real-time PCR using either SYBR Green or TaqMan Probe chemistries.

• Offers exceptional versatility and can be used to extract and amplify DNA from a wide range of sample types
• Compatible with animal tissue, plant, saliva, & bacterial samples
• Extraction & Amplification in less than 60 minutes
• Includes High Fidelity Hot Start Red Dye Master Mix to prevent the risk of nonspecific amplification & to visualize bands easily
• Process extracted sample via PCR with included mastermix
• Alternatively, amplify via qPCR (qPCR mastermix not included)

Each 100 reaction kit includes:

1 tube of High Fidelity Hot Start Red Dye Master Mix (1.25 mL)

1 tube of Fast Extraction Lysis Solution (1.25 mL)

Applications: Extraction & Amplification of Tissue, Plant, Saliva, & Bacteria   

Azure HRP Stripping Buffer

Works by removing the primary and secondary antibodies from the membrane

Stripping buffer for stripping and re-probing chemiluminescent Western blots.

• Provided as a 1x ready-to-use solution
• Size: 500mL

Azure Aqua Transfer Cell

Perform a quick 1 hour transfer or transfer overnight at a lower voltage

Aqua Transfer Cell is used for transferring two mini gels to membranes for Western blotting experiments (wet transfer). The transfer cassettes and electrode core are colored for directionality which makes transferring easy and straightforward.

Includes:

• 2 transfer cassettes
• 6 sponges
• 2 Azure Aqua cooling units
• Tank and upper lid with power cables
• Transfer Electrode Cores

Features

The transfer cassettes and electrode core are colored for directionality, making transferring easy and straightforward.

Chemical reagent compatibility

Keep all components of the Azure Aqua Transfer Cell away from chloroform, arenes (such as methylbenzene benzene), and acetone. Any damage caused by these reagents is not covered by the warranty policy.

 
Cleaning Instructions

Before using, wash all components with a neutral detergent and rinse well with ultra pure water.

Wash the electrode assembly, transfer cassettes, and buffer tank with a neutral detergent and warm water. Be careful to avoid damaging the platinum electrodes. Use warm water to rinse the sponges, and then rinse all components with distilled or ultra-pure water.

Specifications
 

Azure Transfer Buffer

This proprietary transfer buffer provides improved detection of low-abundance and post-translationally modified proteins.

High-efficiency protein transfer and increased protein retention on the membrane add up to more sensitive Western blots.

• Provided as a 50x concentrated solution. Sufficient for 50-100 blots
• Size: 500mL

Features

• High performance—increased protein retention on the membrane and fast transfer times result in more sensitive detection of low-abundance and post-translationally modified proteins
• Efficient—enhanced transfer efficiency for proteins of all sizes
• Fast—transfer proteins in less than 20 minutes vs. several hours required with traditional transfer buffers
• Convenient—use your existing wet transfer electrophoresis apparatus

Azure ChemiWriter ECL Pen

Chemiluminescent pen for blot annotation

The Azure ChemiWriter ECL Western blot annotation pen is a chemiluminescent marker that enables you to write or draw on your transfer membranes, resulting in a chemiluminescent signal on your markings once substrate is added. Contains a reagent that reacts with HRP substrates to produce chemiluminescence and also a blue dye to allow you to see markings as you write on the Western blot.

Use for tracing visible protein ladders or making identifying marks on your Western blots. Pen has two tips on each end: one fine-tipped for thin markings and one thicker for depositing more of the reagent.

Quantity: 1 pen

Features

Enables you to write or draw on your transfer membranes. A chemiluminescent signal will be detectable from your markings once chemiluminescent substrate is added.

• Use for tracing visible protein ladders or making identifying marks on your blots. Pen has two tips on each end: one fine-tipped for thin markings and one thicker for depositing more of the reagent.
• There are two heads to the pen, a fine point tip and a thicker chisel point tip.
• We recommend using the fine point tip to annotate the blot and mark protein ladders for detection.
• The chisel point of the pen can be used to deposit an increased amount of reagent on the blot for more intense signal.
• For best results we recommend annotating your blot after transfer and before starting your Western blotting protocol.
• Once annotated, the blot may be stored dry or wet, refrigerated or at room temperature.
• Storage: pen should be stored tightly closed at room temperature. ChemiWriter ECL reagent and blue ink markings on the blot are stable for at least three months.

Azure Chemi Blot Blocking Buffer

This Chemi Blot Blocking Buffer was formulated to enhance signal and lower background by improving sensitivity. Enhances specific antibody-antigen interactions specifically for chemiluminescent Western blots. 

• Provided as a 1x ready-to-use solution- no mixing or dilutions required.
• Size: 500mL

Features

Western blot membranes should be incubated in blocking buffer before antibody hybridization to prevent non-specific binding of antibodies. Blocking buffer can also be used for diluting primary and secondary antibodies during hybridization.

Azure Aqua Power Supply

Designed for powering electrophoresis and Western blotting transfer modules

This power supply can create and save protocols for all your gel and blotting experiments. Designed with you in mind, features a pause/resume function for flexibility.

Power supply includes output range of 5–600V, 1–1200mA, 1–500W, LCD readable voltage, current, power, and four power connections to run multiple experiments at once. Able to control constant power, current, or voltage.

Features

The Azure Aqua Power Supply is a universal power supply that is designed for powering electrophoresis and transfer modules. The power supply can create and save protocols for all your gel and blotting experiments and includes a pause/resume function for flexibility.

Azure Power Supply includes an output range of 5-600V, 1-1200mA, 1-500W, LCD readable voltage, current, power, and 4 connections for electrophoresis and transfer.

Specifications
 

Azure Blot Washing Buffer

Wash buffers are an important part of the blotting process for removing unhybridized antibody and preventing background signal.

Blot washing buffer for use with Azure Imaging Systems, or any other Western blotting system.

• Provided as a 10x concentrated solution
• Size: 500mL

NOTE: While this Blot Washing Buffer is compatible with NIR Fluorescent Western blots, for the best results with NIR fluorescence, we recommend the Azure Fluorescent Blot Washing Buffer.