PR1MA™ Hot Start Taq

• Exceptional sensitivity for low-copy PCR
• Ideal for multiplex PCR and amplification of GC-rich DNA
• Same enhanced features as PR1MA Taq Polymerase
• Buffer optimized for fast cycling and reproducibility 

Engineered for controlled polymerase activity, PR1MA™ Hot Start Taq is bound with a monoclonal antibody that blocks enzyme activity. This allows reactions to be set up at room temperature without the risk of non-specific amplification.

When samples are ready, the reaction mixture is heated to 95°C to denature the antibody and initiate the reaction. Similar to the standard PR1MA™ Taq, Hot Start Taq is provided with a 5X buffer, or in a ready-to-use 2X Master Mix. The Master Mix can be ordered with or without an incorporated red gel loading dye. The Red Dye Master Mix incorporates a red-loading dye that allows amplified samples to be loaded directly on an agarose gel.  The red color aids in visualization during pipetting, and higher density of the buffer ensures that the samples will drop into the gel wells.

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TC-96 Turbo™ 2D Gradient Thermal Cycler

• Exceptionally fast cycling – speeds up to 9°C/second
• 2D Gradient for simultaneous optimization of annealing and denaturation temperatures
• Large, folding 10” touch screen
• Program wizard for quick and easy protocol entry
• Optimize results with MIDSCI™ PCR reagents

The Benchmark™ TC-96 Turbo™ 2D Gradient Thermal Cycler is designed for fast cycling and quick protocol optimization. Exceptionally easy to program, the unit features tight temperature control and excellent block uniformity for consistent, reliable results.

Many thermal cyclers offer users the option to select a temperature gradient across the block to test a range of annealing temperatures simultaneously. The TC-96 Turbo™ offers a 2D gradient – across both the columns and rows – allowing for optimization of annealing and denaturation temperatures in the same run. The ability to optimize both steps provides for an increase in yield and specificity of complex or GC rich templates. Temperature ramp rates as fast as 9°C per second allow reactions to be performed quickly – up to 20% faster than other popular thermal cyclers. Temperature change is precisely controlled by a proprietary algorithm that simulates sample temperature and allows samples to arrive at the programmed temperature without any lag or overshoot.

All operating parameters are controlled and monitored from the large, full color, 10” touch screen. The screen can be angled toward the user for easy access and folded back against the instrument when not in use to save space. Entering and editing programs is intuitive with the program wizard. Programming features include adjustable ramp rates, temperature and time increments/decrements, password protected multi-user login and a memory for 30,000 programs.

TC-96 Turbo™ will accommodate 96 x 0.2 or 96 x 0.1 mL tubes as well as non-skirted 96 well plates. The height of the heated lid is auto-adjusting to prevent condensation for all of the accepted consumables. Temperature of the lid is adjustable.

Compatible with select MIDSCI™ PCR reagents, click here to see options.

*For research use only.

Specifications

 
MultiCycler™ Multi-block Thermal Cycler

Designed for laboratories wanting flexibility in their applications, Benchmark’s MultiCycler™ Thermal Cyclers offer multiple, independent cycling blocks in a single unit.

• Dual or triple block models are available, both offering extremely fast cycling profiles
• Large touchscreen interfaces
• Self-adjusting heated lids
• Gradient capabilities
• Easy-to-use program wizard.

All programming features are accessed from the large, 10” full-color touch screen. The screen can be angled towards the user when in use and then tucked away to save space. A program wizard guides users through the simple protocol setup process. Advanced programming features include gradient, adjustable ramp rates, and time increments and decrements. For multi-user labs, password protection prevents interference with an existing run. A USB provides for unlimited program storage and easy transfer of protocols between MultiCycler™ machines.

The auto-adjusting heated lid prevents condensation and evaporation of samples. Lid temperature can be programmed, and when the block temperature is below 35°C, the lid automatically shuts off. Peltier units allow for exceptionally fast heating and cooling of the blocks. Temperature change is precisely and accurately controlled within tight tolerances by a proprietary algorithm that simulates sample temperature, resulting in fast runs and reproducible results.
 
The wells are arranged in a 4×8 and 6×8 format, allowing for the use of PCR strip tubes in both models. Each block is independently controlled.

Specifications

myGel InstaView™ System | Complete System with Power Supply

• View DNA migration and separation as it happens
• Blue light illumination - won't damage DNA
• Capture high-resolution images without removing the gel from the tank

The myGel InstaView™ comes complete with everything needed to cast, run, and view mini electrophoresis gels, including an integrated power supply. One large (10.5 x 10 cm) or small (10.5 x 6 cm) gel is accommodated by the gel tank. Gels are easily poured using the supplied trays, combs, and leak-proof casting stand. The power supply connects directly to the gel tank – no more leads to contend with. The connection is guaranteed to be right, every time.

Output voltage can be adjusted to 35V, 50V, or 100V and run time can be set for up to 99 minutes. A magnetic interlock between the gel tank and the lid prevents current from running through the system when the lid is not properly in place.

Once the myGel InstaView™ System is set up, simply slide the mini LED transilluminator into place under the tank and at any time turn it on to view the run in real-time. An array of blue LEDs emits bright blue light at a wavelength of 465 nm for optimal excitation of common green fluorescent stains such as SmartGlow™, SYBYR™ Green, and Gel Green, without damaging DNA. An orange filter is incorporated into the lid for visualizing DNA bands without any additional equipment or special glasses. When it’s time to document results, place the included gel imaging enclosure over top of the myGel InstaView, and use the camera app on any smartphone to capture high-resolution images without removing the gel from the tank.

Images are easily shared via email or text message or printed.
 

Specifications

InstaView™ Gel Tank

For labs that prefer to use a separate power supply, the InstaView™ Gel Tank is the perfect choice for running, viewing, and documenting electrophoretic separations. The InstaView™ Gel Tank accommodates one large (10.5 x 10 cm) or one small (10.5 x 6 cm) gel tray and is supplied with trays, combs, and a leak-proof casting stand. The gel tank lid includes attached power leads which prevent the unit from operating without the lid properly in place.

Any standard power supply can be connected, including the Accuris myVolt™ Mini and myVolt Touch. The InstaView™ Gel Tank is shaped to allow the mini-LED transilluminator (included) to slide underneath. Blue light, at a wavelength of 465 nm, provides excitation of common green fluorescent stains such as SmartGlow™, SYBR™ Green, and GelGreen. An orange filter is incorporated into the gel tank lid for visualizing the DNA in the gel box without any additional equipment or glasses. The transilluminator can be turned on at any time during a run to track progress and automatically shuts off after 5 minutes.

After electrophoresis, simply place the included imaging enclosure over the InstaView™ Gel Tank to capture high-resolution images with a smartphone. Images are easily printed or shared via email or text message.

Specifications
 

Safety	Physical interlock between lid and tank
Buffer Volume	230 mL
Gel Capacity	1-10.5 x 10 cm or 1-10.5 x 6 cm gels, 0.5 cm thick
Transilluminator	Blue LED, 465 nm
WxDxH (w/enclosure)	7.5 x 8.25 x 5 in /19 x 21 x 14 cm
Weight	1.65lb/0.75kg
Electrical	115V, 60Hz or 230V, 50Hz
Warranty	2-year

  

System & Accessories
 

 
PR1MA™ High Fidelity Polymerase

• 50X higher fidelity than Taq DNA polymerase
• Works with crude DNA sample
• Ideal for cloning, mutagenesis and microarrays
• Produces blunt end products, to clone directly into blunt end vectors
• Optimized buffer system with unique PCR enhancers 

For applications requiring highly accurate amplification, choose PR1MA™ High Fidelity DNA polymerase. Modified for better solubility and higher activity across a broad range of ionic conditions, this polymerase will amplify a wide range of targets, including those that are GC or AT rich as well as crude samples.

A 3'-5' proofreading exonuclease activity and an error rate of 4.55×10-7 makes PR1MA™ High Fidelity DNA Polymerase the perfect partner for cloning applications. The supplied 5X buffer with dNTPs is optimized for compatibility with a variety of targets.

Specifications

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PR1MA™ High Fidelity Hot Start Master Mix

• Leaves an A-overhang for TA cloning
• Ideal for difficult, high GC content sequences
• 10x fidelity of native Taq
• Ideal for long PCR, up to 10kb targets 

PR1MA™ High Fidelity Hot Start Mix is a hot start 2x formulation which provides excellent sensitivity in low-copy number assays with 10x higher fidelity than Taq polymerase. The 2x master mix contains proprietary enhancers, an antibody-mediated hot-start mechanism, and a proofreading component for trouble-free PCR reaction assembly and performance.

The pre-optimized Hot Start Master Mix is supplied in a single tube, reducing the number of pipetting steps while improving throughput and reproducibility. The highly efficient buffer formulation and hot-start blend provide the ideal conditions for high-performance PCR and inactivity at room temperature thereby eliminating non-specific amplification.

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PR1MA™  High Fidelity Master Mix

• Leaves a blunt end
• Rapid extension: up to 1 kb per 15 seconds
• 100x fidelity of native Taq
• Ideal for shorter, less complex targets

A 2x formulation which provides extreme sensitivity in low copy number assays with 100x the fidelity of native Taq, PR1MA™ High Fidelity Master Mix is perfect for shorter, less complex targets. The 2x master-mix contains proprietary enhancers and a proof-reading component for trouble-free PCR reaction assembly and performance. High Fidelity Master Mix delivers a unique balance of PCR sensitivity, high fidelity, versatility, and tolerance to inhibitors.

The pre-optimized Master Mix is supplied in a single tube, reducing the number of pipetting steps while improving throughput and reproducibility. The highly efficient buffer formulation provides the ideal conditions for high-performance PCR.

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PR1MA™ qMAX One-Step RT-qPCR Kits

• RNA to cDNA to qPCR in one tube
• High purity enzyme formulation for enhanced stability and performance
• PR1MA™ Hot-Start Taq allows for preparation at room-temperature
• Compatible with all qPCR instruments
• Available for green fluorescence or probe detection

Both One-Step Kits are compatible with standard and fast cycling protocols and provide increased sensitivity, speed and reproducibility for a broad range of samples and targets.  The polymerase mix is available with different levels of ROX reference dye for compatibility with all qPCR instruments.

Two versions of our One-Step qPCR Kits are available:

PR1MA™ qMAX Green One-Step Kits incorporate our proprietary intercalating dye which exhibits higher fluorescent and lower PCR inhibition than other popular dyes such as SYBR.

PR1MA™ qMAX Probe One-Step Kits are optimized for use with popular TaqMan, Scorpions, and molecular beacon probes.

*Please note these products ship on dry ice.  Appropriate shipping charges apply unless otherwise noted on a quote.

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PR1MA™ SmartCheck DNA Ladders

• Ready-to-use formulation includes loading buffer and tracking dye
• 500 µL suitable for 100 lanes (5 µL per lane)
• Higher intensity reference bands
• Ultra pure production allows economical ambient shipping 

Technical Details
 

Protocol: Briefly vortex the tube and use 5 µL per lane. Additional loading buffer is not required.

Concentration: 0.1 mg / mL

Tracking dye: bromophenol blue and xylene cyanol

Buffer formulation: 10mM Tris-HCl (pH 8.0), 5 mM EDTA, 12.5% glycerol, 0.008% bromophenol blue, 0.008% xylene cyanol

Shipping and Storage: SmartCheck™ DNA Ladders are shipped at ambient temperature. On arrival, store at -20°C for optimum stability and long-term storage up to 15 months.

Production: SmartCheck™ ladders are produced from proprietary plasmids, digested to completion. A multistep chromatography method is used to ensure purity and DNA quality.

Quality Control: Each lot of SmartCheck™ DNA Ladders is tested with a spectrophotometer to confirm DNA concentration, as well as agarose electrophoresis to confirm band sharpness.

Specifications 

Item	Description	Volume
PR4005-100	PR1MA™ SmartCheck™ 50bp DNA Ladder	500 uL / 100 Lanes
PR4005-500	PR1MA™ SmartCheck™ 50bp DNA Ladder	5 x 500 uL / 500 Lanes
PR4010-100	PR1MA™ SmartCheck™ 100bp DNA Ladder	500 uL / 100 Lanes
PR4010-500	PR1MA™ SmartCheck™ 100bp DNA Ladder	5 x 500 uL / 500 Lanes
PR4100-100	PR1MA™ SmartCheck™ 1kb DNA Ladder	500 uL / 100 Lanes
PR4100-500	PR1MA™ SmartCheck™ 1kb DNA Ladder	5 x 500 uL / 500 Lanes

  

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PurePlus® PCR Plates

• PurePlus® plates are tested for RNase/DNase lot by lot to ensure pure product
• Our plates are lot certified free of Human DNA contamination
• Our plates are tested for ATP lot by lot to ensure pure consistent product
• Broad product range. Over 18 styles of plates fit most major thermocyclers
• These plates are autoclavable to 122° C for 15 minutes at 15PSI

Ultra Clear and Ultra Clean

PurePlus® sterile disposables, like all our other sterile products, receive valid sterilization processing compliant with ISO 11137. So unlike "pre-sterile" products from other suppliers you can use PurePlus® PCR disposables for diagnostic and regulated procedures.

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PR1MA™ Mammalian Genotyping Kit

• DNA extraction and amplification in 1 hour
• Proprietary lysis buffer optimized for ear punches, tail snips and other mammalian tissues
• Single tube - no organic solvents or clean up procedures
• Enhanced PR1MA™ Hot Start Polymerase included

Traditionally, mammalian genotyping protocols have involved Proteinase K digestion, neutralization, organic extraction and lots of hands on time to get PCR-ready DNA.

The PR1MA™ Mammalian Genotyping Kit is quick and easy to use - add sample to the proprietary lysis buffer and incubate for 5-10 minutes, then add the deactivation buffer and incubate for 10 minutes. The crude lysate can then be amplified using fast PCR PR1MA™ Hot Start Taq Master Mix with red loading dye (included). The kit contains everything needed - just add sample and primers. 
 

PR1MA™ 1 Hour Mammalian Genotyping Kit 

 PR1MA™ Genotyping Hot Start Master Mix, 2X Concentration with Red Dye

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IBI RT-PCR Certified and Nuclease-Free Water is ideal for all applications in a molecular biology lab including PCR, RT-PCR, restriction enzyme
assays, modifying enzyme assays, transfection, cloning, transformation, and all general molecular biology lab procedures.

IBI PCR Grade water is manufactured under stringent conditions. The purification process includes continuous deionization, reverse osmosis,
UV-treatment, 0.2µm filtration, followed by steam sterilization in an autoclave.