PR1MA™ High Fidelity Polymerase

• 50X higher fidelity than Taq DNA polymerase
• Works with crude DNA sample
• Ideal for cloning, mutagenesis and microarrays
• Produces blunt end products, to clone directly into blunt end vectors
• Optimized buffer system with unique PCR enhancers 

For applications requiring highly accurate amplification, choose PR1MA™ High Fidelity DNA polymerase. Modified for better solubility and higher activity across a broad range of ionic conditions, this polymerase will amplify a wide range of targets, including those that are GC or AT rich as well as crude samples.

A 3'-5' proofreading exonuclease activity and an error rate of 4.55×10-7 makes PR1MA™ High Fidelity DNA Polymerase the perfect partner for cloning applications. The supplied 5X buffer with dNTPs is optimized for compatibility with a variety of targets.

Specifications

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StripSpin 12 Mini Centrifuge has been discontinued. A replacement model (C2248) can be found here.

StripSpin 12 Mini Centrifuge

• Unique rotor for 12-strip PCR tubes
• Also can be used with 8-strip and individual PCR tubes
• Starts and Stops with opening/closing the lid
• Tiny benchtop footprint and affordability
• Place a StripSpin at the bench, thermal cycler & water bath for convenience.

Specifications:

 
PR1MA™ High Fidelity Hot Start Master Mix

• Leaves an A-overhang for TA cloning
• Ideal for difficult, high GC content sequences
• 10x fidelity of native Taq
• Ideal for long PCR, up to 10kb targets 

PR1MA™ High Fidelity Hot Start Mix is a hot start 2x formulation which provides excellent sensitivity in low-copy number assays with 10x higher fidelity than Taq polymerase. The 2x master mix contains proprietary enhancers, an antibody-mediated hot-start mechanism, and a proofreading component for trouble-free PCR reaction assembly and performance.

The pre-optimized Hot Start Master Mix is supplied in a single tube, reducing the number of pipetting steps while improving throughput and reproducibility. The highly efficient buffer formulation and hot-start blend provide the ideal conditions for high-performance PCR and inactivity at room temperature thereby eliminating non-specific amplification.

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PR1MA™  High Fidelity Master Mix

• Leaves a blunt end
• Rapid extension: up to 1 kb per 15 seconds
• 100x fidelity of native Taq
• Ideal for shorter, less complex targets

A 2x formulation which provides extreme sensitivity in low copy number assays with 100x the fidelity of native Taq, PR1MA™ High Fidelity Master Mix is perfect for shorter, less complex targets. The 2x master-mix contains proprietary enhancers and a proof-reading component for trouble-free PCR reaction assembly and performance. High Fidelity Master Mix delivers a unique balance of PCR sensitivity, high fidelity, versatility, and tolerance to inhibitors.

The pre-optimized Master Mix is supplied in a single tube, reducing the number of pipetting steps while improving throughput and reproducibility. The highly efficient buffer formulation provides the ideal conditions for high-performance PCR.

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PR1MA™ qMAX One-Step RT-qPCR Kits

• RNA to cDNA to qPCR in one tube
• High purity enzyme formulation for enhanced stability and performance
• PR1MA™ Hot-Start Taq allows for preparation at room-temperature
• Compatible with all qPCR instruments
• Available for green fluorescence or probe detection

Both One-Step Kits are compatible with standard and fast cycling protocols and provide increased sensitivity, speed and reproducibility for a broad range of samples and targets.  The polymerase mix is available with different levels of ROX reference dye for compatibility with all qPCR instruments.

Two versions of our One-Step qPCR Kits are available:

PR1MA™ qMAX Green One-Step Kits incorporate our proprietary intercalating dye which exhibits higher fluorescent and lower PCR inhibition than other popular dyes such as SYBR.

PR1MA™ qMAX Probe One-Step Kits are optimized for use with popular TaqMan, Scorpions, and molecular beacon probes.

*Please note these products ship on dry ice.  Appropriate shipping charges apply unless otherwise noted on a quote.

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PR1MA™ SmartCheck DNA Ladders

• Ready-to-use formulation includes loading buffer and tracking dye
• 500 µL suitable for 100 lanes (5 µL per lane)
• Higher intensity reference bands
• Ultra pure production allows economical ambient shipping 

Technical Details
 

Protocol: Briefly vortex the tube and use 5 µL per lane. Additional loading buffer is not required.

Concentration: 0.1 mg / mL

Tracking dye: bromophenol blue and xylene cyanol

Buffer formulation: 10mM Tris-HCl (pH 8.0), 5 mM EDTA, 12.5% glycerol, 0.008% bromophenol blue, 0.008% xylene cyanol

Shipping and Storage: SmartCheck™ DNA Ladders are shipped at ambient temperature. On arrival, store at -20°C for optimum stability and long-term storage up to 15 months.

Production: SmartCheck™ ladders are produced from proprietary plasmids, digested to completion. A multistep chromatography method is used to ensure purity and DNA quality.

Quality Control: Each lot of SmartCheck™ DNA Ladders is tested with a spectrophotometer to confirm DNA concentration, as well as agarose electrophoresis to confirm band sharpness.

Specifications 

Item	Description	Volume
PR4005-100	PR1MA™ SmartCheck™ 50bp DNA Ladder	500 uL / 100 Lanes
PR4005-500	PR1MA™ SmartCheck™ 50bp DNA Ladder	5 x 500 uL / 500 Lanes
PR4010-100	PR1MA™ SmartCheck™ 100bp DNA Ladder	500 uL / 100 Lanes
PR4010-500	PR1MA™ SmartCheck™ 100bp DNA Ladder	5 x 500 uL / 500 Lanes
PR4100-100	PR1MA™ SmartCheck™ 1kb DNA Ladder	500 uL / 100 Lanes
PR4100-500	PR1MA™ SmartCheck™ 1kb DNA Ladder	5 x 500 uL / 500 Lanes

  

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PR1MA™ Mammalian Genotyping Kit

• DNA extraction and amplification in 1 hour
• Proprietary lysis buffer optimized for ear punches, tail snips and other mammalian tissues
• Single tube - no organic solvents or clean up procedures
• Enhanced PR1MA™ Hot Start Polymerase included

Traditionally, mammalian genotyping protocols have involved Proteinase K digestion, neutralization, organic extraction and lots of hands on time to get PCR-ready DNA.

The PR1MA™ Mammalian Genotyping Kit is quick and easy to use - add sample to the proprietary lysis buffer and incubate for 5-10 minutes, then add the deactivation buffer and incubate for 10 minutes. The crude lysate can then be amplified using fast PCR PR1MA™ Hot Start Taq Master Mix with red loading dye (included). The kit contains everything needed - just add sample and primers. 
 

PR1MA™ 1 Hour Mammalian Genotyping Kit 

 PR1MA™ Genotyping Hot Start Master Mix, 2X Concentration with Red Dye

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IBI RT-PCR Certified and Nuclease-Free Water is ideal for all applications in a molecular biology lab including PCR, RT-PCR, restriction enzyme
assays, modifying enzyme assays, transfection, cloning, transformation, and all general molecular biology lab procedures.

IBI PCR Grade water is manufactured under stringent conditions. The purification process includes continuous deionization, reverse osmosis,
UV-treatment, 0.2µm filtration, followed by steam sterilization in an autoclave.

ILP Deep Well Round Well, U-Bottom 42 mm Plates - 96
  

This round deep-well U-bottom plate combines 1.2 mL working volume with automation friendly design. Sturdy and warp-resistant design with ultra-flat surfaces and rigid construction makes this plate is ideal for high throughput screening, sample collection, compound storage, and combinatorial chemistry applications. It is compatible with stackers, grippers, heater/cooler blocks, and other instruments that comply with the ANSI/SLAS 2004-1 (SBS) footprint.

Raised rims on each well help protect from cross-contamination, minimize sample loss during storage, and make for enhanced sealing with adhesive films, silicone mats, or heat sealing films. Manufactured of inert, chemically resistant, and exceptionally clear virgin polypropylene.
 
Made from medical grade virgin polypropylene (no mold release agents or regrind); certified RNase, DNase, DNA, and Pyrogen Free.

• Uniform wall thickness
• Easy well identification with molded in alpha-numeric position markings
• Raised rims for easy and secure sealing with adhesive films, silicone mats, or heat sealing films
• Raised rims help protect from cross contamination

Specifications
 

Number of Wells	96
Number of Plugs	96
Plug Shape	Round – U
Well Shape Top	Round
Well Shape Bottom	Round – U
Material	USP Class VI Polypropylene
Adhesive Material	None
Color	Natural
Alphanumeric	Molded in
Compliance	ANSI/SBS 6-2012
Surface Treatment	None
Certified	DNase-/RNase-/Pyrogen-Free
Overall Height	1.67 In. | 42.50 mm
Well Depth	1.54 In. | 39.05 mm
Overall Length	5.03 In. | 127.78 mm
Adhesive Thickness	None
Width	3.37 In. | 85.48 mm
Top Width	3.24 In. | 82.20 mm
Well Diameter Bottom	0.25 In. | 6.24 mm
Well Diameter Top	0.28 In. | 7.00 mm
Top Length	4.90 In. | 124.48 mm
Well Bottom To Deck	0.14 In. | 3.45 mm
Max Vol.	1.3 mL
Working Vol.	1.2 mL
Vol. With Mat Lid	1.1 mL
Plug Depth	1.54 In. | 39.05 mm
Plug Diameter	0.25 In. | 6.24 mm
Autoclavable	121ºC at 15 psi, 15 min. (249.8ºF)
Max RCF	4,000 RCF
Max Temp.	120ºC (248ºF)
Min Temp.	-80ºC (-112ºF)

ILP Deep Well Square Well, U-Bottom 44 mm Plates - 24

Our 10.0 mL and 15.0 mL 24-well plates are ideal for growing bacterial cultures and for sample purification with magnetic beads, utilizing manual and automated systems. The unique square top with round bottom design allows for optimum mixing and enhanced recovery of magnetic beads. Separated round well bottoms allow for the use of post or ring-style magnetic plates.

Automation-compliant footprint and rigid construction allow for use on automation platforms with most robotic grippers. Raised rims on each well make for easy and secure sealing with adhesive films, silicone mats, or heat-sealing films.

 
Made from Virgin Polypropylene (no mold release agents or regrind); certified RNase, DNase, DNA, and Pyrogen Free.

• Uniform wall thickness
• Easy well identification with molded-in alpha-numeric position markings
• Large sidewalls for easy labeling
• Square well design maximizes sample volume to well ratio while still complying with ANSI/SLAS 6-2012 I

Specifications
 

Number of Wells	24
Number of Plugs	24
Plug Shape	Round – U
Well Shape Top	Square
Well Shape Bottom	Round – U
Material	USP Class VI Polypropylene
Color	Natural
Alphanumeric	Molded in
Compliance	ANSI/SBS 6-2012
Surface Treatment	None
Certified	DNase-/RNase-/Pyrogen-Free
Overall Height	1.76 In. | 44.60 mm
Well Depth	1.61 In. | 41.00 mm
Overall Length	5.03 In. | 127.76 mm
Width	3.37 In. | 85.48 mm
Top Width	3.22 In. | 81.70 mm
Well/Plug Diameter Bottom	0.65 x 0.65 In. | 16.40 x 16.40 mm
Well/Plug Diameter Top	0.67 x 0.67 In. | 17.00 x 17.00 mm
Top Length	4.89 In. | 124.10 mm
Well Bottom To Deck	0.09 In. | 2.40 mm
Max Volume	10 or 15 mL
Working Volume	8.6 mL
Volume With Mat Lid	7.5 mL
Autoclavable	121ºC at 15 psi, 15 min. (249.8ºF)
Max RCF	4,000 RCF
Max Temp.	120ºC (248ºF)
Min Temp.	-80ºC (-112ºF)

 

ILP Deep Well Square Well, U-Bottom Plates - 96

These 96-deep well plates are ideal for separations of nucleic acids and clean-up procedures with magnetic beads. The unique square top with a round bottom design allows for maximum volume, optimum mixing, and enhanced separation/recovery of magnetic beads. Separated round well bottoms allow for the use of ring or post-style magnetic plates.

Raised rims on each well make for easy and secure sealing with adhesive films, silicone mats, or heat-sealing films. Manufactured from virgin polypropylene (no mold release agents or regrind) and certified to be free of RNase, DNase, and Pyrogen contamination.

• Uniform wall thickness
• Easy well identification with molded-in alpha-numeric position markings
• Raised rims for easy and secure sealing with adhesive films, silicone mats, or heat-sealing films
• Raised rims help protect from cross-contamination 

Specifications
 

Number of Wells	96
Number of Plugs	96
Plug Shape	Round – U
Well Shape Top	Square
Well Shape Bottom	Round – U
Material	USP Class VI Polypropylene
Adhesive Material	None
Color	Natural
Alphanumeric	Molded in
Compliance	ANSI/SBS 6-2012
Surface Treatment	None
Certified	DNase-/RNase-/Pyrogen-Free
Autoclavable	121ºC (249.8ºF) at 15 psi, 15 min.
Max RCF	4,000 RCF
Max Temp.	120ºC (248ºF)
Min Temp.	-80ºC (-112ºF)