Our rack is perfect for pre- or post-PCR® sampling applications. Sturdy polypropylene rack accommodates individual 0.2mL tubes or strips of 8 or 12 tubes. Tube wells are easily identified with imprinted numbers and letters. Features a removable hinged lid. Available in natural, blue, green, pink, yellow, or assorted colors (one of each). Packs of 5. PCR® is a registered trademark of Hoffman-LaRoche Inc.

PR1MA™ Mini Gel Electrophoresis System

• Complete system, includes gel box, power supply and casting sets
• Quick and easy gel casting, no tape, clamps or spacers required
• Compact design, footprint: 7 x 5.1 in.

The PR1MA™ Mini Gel electrophoresis system includes all of the equipment that you need to get up and running: gel tank, power supply, and two casting sets. Ideal for personal use, small laboratories, or the classroom, this gel box/power supply combination is compact and easy to use.

The mini gel box attaches directly to the power supply for a secure connection every time. Selectable parameters include 3 preset voltages – 35V, 50V or 100V – and a 99-minute timer. All are set using the membrane keypad.

The safety lid is vented to help dissipate heat generated during electrophoresis and features a viewing area above the gel. Current will only run through the system when the lid is properly in place on top of the gel box.

Trays and casting stands for both 10.5 x 6 cm and 5 x 6 cm gels are included with the PR1MA™ Mini system, as are double-sided combs. The smaller tooth combs are compatible with multi-channel pipettes. Casting is quick and easy – just place the tray in the casting stand and pour. The trays feature a dark contrast strip to help visualize wells while loading and lines that fluoresce under UV and blue light to track the progress of the run.

Specifications

• Convenient cDNA synthesis and PCR in a single tube from 1 pg total RNA
• Formulated for highly specific and sensitive RT-PCR from any RNA templates
• Incorporates thermostable reverse transcriptase and Hot-Start Taq for preparation at room temperature

The PR1MA™ One-Step RT-PCR Kit has been formulated for cDNA synthesis and subsequent PCR in a single tube for end-point analysis. This latest generation RT-PCR Kit consists of an MMLV-derived, thermostable Reverse Transcriptase (45°C to 55°C), an advanced RNase Inhibitor and PR1MA™ Hot Start Taq for ultra-sensitive one-step RT-PCR from as little as 1pg total RNA starting material.

The optimized buffer chemistry allows for efficient reverse transcription and PCR of problematic sequences with significant secondary structure (such as GC-rich targets). The PR1MA One-Step RT-PCR Kit is ideal for determining the presence or absence of RNA templates and quantifying expression through qualitative analysis of RNA transcription levels. The kit also efficiently synthesizes double-stranded cDNA for subsequent gene expression analysis.

PR1MA™ qMAX™ cDNA Synthesis Kits

PR1MA™ now offers two cDNA Synthesis Kits, to meet a range of requirements.
 
The original cDNA Synthesis Kit is a 2-tube format for easy reaction setup and is ideal for 4 pg to 0.5 µg of input RNA. One tube includes our exceptionally stable Reverse Transcriptase combined with a potent RNAse inhibitor, and the other tube contains a 5X reaction buffer with an optimal mixture of anchored oligo (dT) primers and random hexamers to produce a non-biased population of cDNA. 
 
The 1st Strand cDNA Synthesis Flex Kit includes four components: a high-capacity Reverse Transcriptase, an optimized 5X buffer, separate solutions of oligo (dT) primers, and random hexamer primers. This multi-component format is ideal for 10pg to 2.0µg of input RNA and allows for greater flexibility in assay design.

 
PR1MA™ qMax cDNA Synthesis Kits
  

Item	Description	Volume (20 µL)
PR2100-C-S	PR1MA™ qMax™ cDNA Synthesis Kit	10 Reactions (Sample)
PR2100-C-25	PR1MA™ qMax™ cDNA Synthesis Kit	25 Reactions
PR2100-C-100	PR1MA™ qMax™ cDNA Synthesis Kit	100 Reactions
PR2100-C-250	PR1MA™ qMax™ cDNA Synthesis Kit	250 Reactions

 

PR1MA™ qMax First Strand cDNA Synthesis Flex Kits  
 

Item	Description	Volume (20 µL)
PR2110-S	PR1MA™ qMax™ First Strand cDNA Synthesis Flex Kit	10 Reactions (Sample)
PR2110-50	PR1MA™ qMax™ First Strand cDNA Synthesis Flex Kit	50 Reactions
PR2110-100	PR1MA™ qMax™ First Strand cDNA Synthesis Flex Kit	100 Reactions
PR2110-200	PR1MA™ qMax™ First Strand cDNA Synthesis Flex Kit	200 Reactions

 Item PR2110-100 has been discontinued by the manufacturer;
however, we will be continuing to supply the other quantities.

In place of item PR2110-100, please order items PR2110-50 or PR2110-200

 

 
PR1MA™ dNTPs

• Supplied as a ready-to-use 40 mM mix or a set of 4 separate 100 mM solutions
• Free of impurities and inhibitors that reduce sensitivity and yield
• No nuclease, protease or nickase activity
• >99% pure, purified by HPLC
• 24-month shelf life

PR1MA™ dNTP's are purified by HPLC in a strict process that results in greater than 99% purity. The stringent purification process eliminates PCR inhibitors such as tetraphosphates and pyrophosphates that can interfere with the sensitivity of your PCR and reduce yields.
The 40 mM dNTP Mix is a single tube that contains premixed dNTPs at a concentration of 10 mM each. The 100 mM dNTP Set contains four individual tubes, one each of dATP, dCTP, dGTP and dTTP. The nucleotides are supplied in ultra-pure water as an ammonium salt. Both the set and mix are stable for 24 months when stored at -20ºC.

Extensive quality control testing is performed to ensure the dNTPs are free of nuclease, protease and nickase activity. Each lot is performance tested in standard PCR, long PCR and qPCR reactions to assess reproducibility and sensitivity.

PR1MA™ dNTP Mix

PR1MA™ dNTP Set  

Item	Description	Volume
PR3101-S-1	PR1MA™ 100 mM dNTP Set, dATP, dCTP, dGTP, dTTP	250 uL / 25 umol each
PR3101-S-4	PR1MA™ 100 mM dNTP Set, dATP, dCTP, dGTP, dTTP	1 mL / 100 umol each
PR3101-S-20	PR1MA™ 100 mM dNTP Set, dATP, dCTP, dGTP, dTTP	20 x 250 uL / 25 umol each

 

Mystaire® CleanPrep™ dead air boxes are circulation-free enclosures that establish an ideal work area to protect against contamination in sensitive PCR amplification reactions. CleanPrep™ workstations are constructed from thermally-fused polypropylene that offers exceptional chemical resistance, does not adsorb moisture and can be easily cleaned and decontaminated between amplifications using a variety of disinfectant solutions.Seamless polypropylene design for easy decontamination between amplifications.

FEATURES:

1. Clear polycarbonate access with overlap and safety position switch
2. UV shelf with integrated pipette holder - accommodates four pipettors
3. High intensity fluorescent lamps and shortwave UV light
4. Available in 36 and 48 inch widths with nominal 24 inch depth
5. Circulation-free enclosure for PCR applications

HEPA Filters, Pre-Filters, UV bulbs, additional ports and shelves also available.

 
PR1MA™ Hot Start Taq

• Exceptional sensitivity for low-copy PCR
• Ideal for multiplex PCR and amplification of GC-rich DNA
• Same enhanced features as PR1MA Taq Polymerase
• Buffer optimized for fast cycling and reproducibility

Engineered for controlled polymerase activity, PR1MA™ Hot Start Taq is bound with a monoclonal antibody that blocks enzyme activity. This allows reactions to be set up at room temperature without the risk of non-specific amplification.

When samples are ready, the reaction mixture is heated to 95°C to denature the antibody and initiate the reaction. Similar to the standard PR1MA Taq, Hot Start Taq is provided with a 5X buffer, or in a ready-to-use 2X Master Mix. The Master Mix can be ordered with or without an incorporated red gel loading dye.  The Red Dye Master Mix incorporates a red-loading dye that allows amplified samples to be loaded directly on an agarose gel.  The red color aids in visualization during pipetting, and higher density of the buffer ensures that the samples will drop into the gel wells.

 
MultiCycler™ Multi-block Thermal Cycler

Designed for laboratories wanting flexibility in their applications, Benchmark’s MultiCycler™ Thermal Cyclers offer multiple, independent cycling blocks in a single unit.

• Dual or triple block models are available, both offering extremely fast cycling profiles
• Large touchscreen interfaces
• Self-adjusting heated lids
• Gradient capabilities
• Easy-to-use program wizard.

All programming features are accessed from the large, 10” full-color touch screen. The screen can be angled towards the user when in use and then tucked away to save space. A program wizard guides users through the simple protocol setup process. Advanced programming features include gradient, adjustable ramp rates, and time increments and decrements. For multi-user labs, password protection prevents interference with an existing run. A USB provides for unlimited program storage and easy transfer of protocols between MultiCycler™ machines.

The auto-adjusting heated lid prevents condensation and evaporation of samples. Lid temperature can be programmed, and when the block temperature is below 35°C, the lid automatically shuts off. Peltier units allow for exceptionally fast heating and cooling of the blocks. Temperature change is precisely and accurately controlled within tight tolerances by a proprietary algorithm that simulates sample temperature, resulting in fast runs and reproducible results.
 
The wells are arranged in a 4×8 and 6×8 format, allowing for the use of PCR strip tubes in both models. Each block is independently controlled.

Specifications

myGel InstaView™ System | Complete System with Power Supply

• View DNA migration and separation as it happens
• Blue light illumination - won't damage DNA
• Capture high-resolution images without removing the gel from the tank

The myGel InstaView^™ comes complete with everything needed to cast, run, and view mini electrophoresis gels, including an integrated power supply. One large (10.5 x 10 cm) or small (10.5 x 6 cm) gel is accommodated by the gel tank. Gels are easily poured using the supplied trays, combs, and leak-proof casting stand. The power supply connects directly to the gel tank – no more leads to contend with. The connection is guaranteed to be right, every time.

Output voltage can be adjusted to 35V, 50V, or 100V and run time can be set for up to 99 minutes. A magnetic interlock between the gel tank and the lid prevents current from running through the system when the lid is not properly in place.

Once the myGel InstaView System is set up, simply slide the mini LED transilluminator into place under the tank and at any time turn it on to view the run in real-time. An array of blue LEDs emits bright blue light at a wavelength of 465 nm for optimal excitation of common green fluorescent stains such as SmartGlow^™, SYBYR^™ Green, and Gel Green, without damaging DNA. An orange filter is incorporated into the lid for visualizing DNA bands without any additional equipment or special glasses. When it’s time to document results, place the included gel imaging enclosure over top of the myGel InstaView, and use the camera app on any smartphone to capture high-resolution images without removing the gel from the tank.

Images are easily shared via email or text message or printed.
 

Specifications

InstaView Gel Tank

For labs that prefer to use a separate power supply, the InstaView Gel Tank is the perfect choice for running, viewing, and documenting electrophoretic separations. The InstaView Gel Tank accommodates one large (10.5 x 10 cm) or one small (10.5 x 6 cm) gel tray and is supplied with trays, combs, and a leak-proof casting stand. The gel tank lid includes attached power leads which prevent the unit from operating without the lid properly in place.

Any standard power supply can be connected, including the Accuris myVolt^™ Mini and myVolt Touch. The InstaView Gel Tank is shaped to allow the mini-LED transilluminator (included) to slide underneath. Blue light, at a wavelength of 465 nm, provides excitation of common green fluorescent stains such as SmartGlow^™, SYBR^™ Green, and GelGreen. An orange filter is incorporated into the gel tank lid for visualizing the DNA in the gel box without any additional equipment or glasses. The transilluminator can be turned on at any time during a run to track progress and automatically shuts off after 5 minutes.

After electrophoresis, simply place the included imaging enclosure over the InstaView Gel Tank to capture high-resolution images with a smartphone. Images are easily printed or shared via email or text message.

Specifications 

Safety	Physical interlock between lid and tank
Buffer Volume	230 mL
Gel Capacity	1-10.5 x 10 cm or 1-10.5 x 6 cm gels, 0.5 cm thick
Transilluminator	Blue LED, 465 nm
WxDxH (w/enclosure)	7.5 x 8.25 x 5 in /19 x 21 x 14 cm
Weight	1.65lb/0.75kg
Electrical	115V, 60Hz or 230V, 50Hz
Warranty	2-year

  

System & Accessories

 
PR1MA™ High Fidelity Polymerase

• 50X higher fidelity than Taq DNA polymerase
• Works with crude DNA sample
• Ideal for cloning, mutagenesis and microarrays
• Produces blunt end products, to clone directly into blunt end vectors
• Optimized buffer system with unique PCR enhancers

For applications requiring highly accurate amplification, choose PR1MA™ High Fidelity DNA polymerase. Modified for better solubility and higher activity across a broad range of ionic conditions, this polymerase will amplify a wide range of targets, including those that are GC or AT rich as well as crude samples.

A 3'-5' proofreading exonuclease activity and an error rate of 4.55×10-7 makes PR1MA™ High Fidelity DNA Polymerase the perfect partner for cloning applications. The supplied 5X buffer with dNTPs is optimized for compatibility with a variety of targets.

 

Specifications