Hoefer® Glass and Alumina Plates for Mini Vertical Systems

Small Glass and Alumina Plates for Mini Vertical Units.

Compatible with SE300 MiniVE, SE260, and the SE250 Series Vertical Electrophoresis Systems. (see notes below). 

This set includes precision-crafted glass and alumina plates for electrophoresis applications. Includes a variety of notched and rectangular plates in 10 x 8 cm and 10 x 10.5 cm sizes, ideal for customizing your vertical gel setup.

*Alumina plates are not recommended for use with the Hoefer® miniVE™ Vertical Protein Electrophoresis and Blotting System. 
 

Specifications
 

Item#	Description	Plate Size	Weight	Qty
SE202P-10	Rectangular Glass Plates	3.94 x 3.15 In. | 10 x 8 cm	0.66 lb | 0.3 kg	10/pk
SE202GN-5	Notched Glass Plates	3.94 x 3.15 In. | 10 x 8 cm	0.44 lb | 0.2 kg	5/pk
SE262P-5	Rectangular Glass Plates	3.94 x 4.13 In. | 10 x 10.5 cm	0.66 lb | 0.3 kg	5/pk
SE262GN-5	Notched Glass Plates	3.94 x 4.13 In. | 10 x 10.5 cm	0.66 lb | 0.3 kg	5/pk
SE202N	Notched Alumina Plate	3.94 x 3.15 In. | 10 x 8 cm	0.22 lb | 0.1 kg	1/ea
SE202N-10	Notched Alumina Plates	3.94 x 3.15 In. | 10 x 8 cm	0.44 lb | 0.2 kg	10/pk
SE262N-5	Notched Alumina Plates	3.94 x 4.13 In. | 10 x 10.5 cm	0.44 lb | 0.2 kg	5/pk

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Adjustable Well Plate Stand

Achieves accurate pipetting by allowing the flexibility to adjust the angle of the microplate (45° max. tilt). The stand takes up little benchtop space measuring only 6 x 4.5 x 2.5 In. (D x W x H).

Proudly made in the USA! Click here for more made in America products!
  

Hoefer® Spacers for Dual and Air Cooled Vertical Systems

Gel Spacers for SE400 and SE600 Series Vertical Electrophoresis Systems.

Engineered for consistent gel casting, these precision-machined spacers are made from high-strength, chemically resistant plastics. Available in three lengths (8, 16, and 24 cm) and multiple thicknesses (0.75 mm, 1.0 mm, 1.5 mm) to accommodate various gel formats.

All 1.0 mm spacers are white for quick identification during setup.

Specifications
 

Item #	Thickness	Length	Width
SE6419-2-75	0.030 In. | 0.75 mm	3.15 In. | 8 cm	0.79 In. | 2 cm
SE6419-2-10	0.039 In. | 1 mm	3.15 In. | 8 cm	0.79 In. | 2 cm
SE6419-2-15	0.059 In. | 1.5 mm	3.15 In. | 8 cm	0.79 In. | 2 cm
SE6119-2-75	0.030 In. | 0.75 mm	6.30 In. | 16 cm	0.79 In. | 2 cm
SE6119-2-10	0.039 In. | 1 mm	6.30 In. | 16 cm	0.79 In. | 2 cm
SE6119-2-15	0.059 In. | 1.5 mm	6.30 In. | 16 cm	0.79 In. | 2 cm
SE6619-2-75	0.030 In. | 0.75 mm	9.45 In. | 24 cm	0.79 In. | 2 cm
SE6619-2-10	0.039 In. | 1 mm	9.45 In. | 24 cm	0.79 In. | 2 cm
SE6619-2-15	0.059 In. | 1.5 mm	9.45 In. | 24 cm	0.79 In. | 2 cm
SE6118-2-10	0.039 In. | 1 mm	6.30 In. | 16 cm	0.39 In. | 1 cm
SE6118-2-15	0.059 In. | 1.5 mm	6.30 In. | 16 cm	0.39 In. | 1 cm

Hoefer® T-Spacers for Mini Vertical Systems

Compatible with SE300 MiniVE, SE260, and the SE250 Series Vertical Electrophoresis Systems.

Ensure accurate gel thickness and leak-free casting with precision-milled T-spacers. All spacers are precision-machined from durable plastics for high chemical resistance and optimum tensile strength. These spacers provide consistent alignment and long-term durability for mini vertical systems.

Available in multiple lengths (8 cm and 10.5 cm) and thicknesses (0.75 mm, 1.0 mm, and 1.5 mm) to suit your specific gel requirements.

Specifications
 

Item #	Thickness	Length	Width
SE2119T-2-75	0.030 In. | 0.75 mm	3.15 In. | 8 cm	0.39 In. | 1 cm
SE2119T-2-10	0.039 In. | 1 mm	3.15 In. | 8 cm	0.39 In. | 1 cm
SE2119T-2-15	0.059 In. | 1.5 mm	3.15 In. | 8 cm	0.39 In. | 1 cm
SE2619T-2-75	0.03 In. | 0.75 mm	4.13 In. | 10.5 cm	0.39 In. | 1 cm
SE2619T-2-10	0.039 In. | 1 mm	4.13 In. | 10.5 cm	0.39 In. | 1 cm
SE2619T-2-15	0.059 In. | 1.5 mm	4.13 In. | 10.5 cm	0.39 In. | 1 cm

Multi-colored 96 Well Orienter

For easy identification of samples in microplates, vertically or horizontally oriented bright colors make it easy to distinguish between columns. Fits most standard flat and round bottom 96-well plates. Available in horizontal and vertical orientations.
 

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This brand is being discontinued and will only be available while supplies last.

Need a great DNA Safe Stain at a great price?
Try PR1MA! Click here to order.

Bullseye DNA SafeStain,10,000x 

• Safest DNA stain by far
• Replaces EthBr in agarose gel electrophoresis
• Detection of DNA and RNA
• Excitation at 290 nm & 490 nm
• Emission at 530 nm

DNA SafeStain is a new and safe nucleic acid stain for visualization of double-stranded DNA, single-stranded DNA, and RNA in agarose gels. The dyes are developed to replace toxic ethidium bromide (EthBr, a potent mutagen), commonly used in gel electrophoresis for visualization of nucleic acids in agarose gels. DNA SafeStain is non-carcinogenic by the Ames-test. The results are negative in both the mouse marrow chromophilous erthrocyte micronucleus and mouse spermary spermatocyte chromosomal aberration tests.

DNA SafeStain emits green fluorescence when bound to DNA. It has two excitation wavelength peaks when bound to nucleic acid, at 290nm and 490nm and the emission can be detected at 530nm.

Storage:
Store under dark at 4°C or room temperature.

User Instruction: 
DNA SafeStain can be used in the exact same way as EtBr in agarose gel electrophoresis. For example, add 100µl to 1 liter of 1X running buffer (1xTAE or 1x TBE), and use this DNA SafeStain containing buffer to make the agarose gel and run the gel.

Special Notes: 
1. For best results, dilute the DNA SafeStain 1:10,000 in your gel running buffer and use this dye-containing running buffer to make your agarose gel and to run the gel.

2. As is the case with EtBr; add more DNA SafeStain, if brighter DNA bands are desired. To view the results, any conventional DNA gel viewing light box or gel documentation system should work well with the DNA SafeStain; although, using a LED light instead of a UV light source is preferred due to the harmful nature of UV light.

This Product is For Research Use Only

*For the proper disposal of this product, follow University or Company Guidelines.

 Bullseye Individual dNTP's

• Ready-to-use molecular grade dNTP solution for use in DNA polymerization, DNA labeling and sequencing processes
• High purity: >98% by HPLC
• Supplied in solution at pH 7.5
• dNTPs are stable at -20°C, avoid multiple freeze/thawing (For long-term usage, aliquoting is recommended)
• Functionally tested with thermostable polymerases

• Mix of dATP, dCTP, dGTP, dTTP
• Each nucleotide is at a concentration of 100 mM
• Ready-to-use molecular grade dNTP solution for use in DNA polymerization, DNA labelling and sequencing processes
• High purity: > 98% by HPLC
• Supplied in solution at pH 7.5
• dNTPs are stable at - 20°C, avoid multiple freeze/thawing (For long-term usage, aliquoting is recommended)
• Functionally tested with thermostable polymerases 

dNTP SET

(dATP, dCTP, dGTP, dTTP)

100 mM

This brand is being discontinued and will only be available while supplies last.
 

Need a great DNA Safe Stain at a great price?
Try PR1MA! Click here to order.
 

Bullseye DNA Safe Stain Plus

• Higher sensitivity
• Use in the same way as EtBr in agarose gel electrophoresis
• Detection of DNA and RNA
• Safest DNA stain by far
• Low cost
• 10,000X
• Excitation wavelengths at 290nm and 490nm

DNA SafeStain Plus is a highly sensitive green fluorescent DNA/RNA staining reagent for detecting nucleic acids in agarose and polyacrylamide gels. This unique stain gives high sensitivity for detection of double-stranded or single-stranded DNA and RNA. Gels can be post-stained or the stain can be added to gels during gel casting or to the gel running buffer. DNA SafeStain Plus has two excitation wavelength peaks at about 290nm and 490nm, and an emission wavelength at 530nm, making it compatible with a standard UV light box, a blue-light transilluminator, or a gel reader equipped with visible light excitation; such as, a 488 nm laser-based gel scanner.

DNA SafeStain Plus is in a 10,000X concentrated format that can be easily diluted 10,000 times for use in precast gel staining, or 5,000 times for use in post gel staining.

Bullseye RT 2X Master Mix

• Up to 9kb cDNA synthesis
• Ensures sample to sample consistency
• Large RNA sample volume capacity
• Ready to use

Size: 100 rxns

RT 2X Master Mix is a proprietary, ready-to-use master mix for first-strand cDNA synthesis in a 2X concentration. This optimized reaction mix contains ribonuclease inhibitor, dNTPs, and a balanced concentration for oligo(dT) and random primers. The ribonuclease inhibitor effectively protects RNA template from degradation. The oligo(dT) anneals selectively to the poly(A) tail of mRNAs and the random primers do not require the presence of poly(A) and they are utilized for the transcription of mRNA 5-end regions. The resultant cDNA can be directly used as template in different PCR experiments.

Kit Components

Storage

• Store at -20°C in a frost-free freezer.

Application

• cDNA synthesis
• Construction of cDNA libraries
• Generation of probes for hybridization 

Protocol

1. Thaw RNA templates and all reagents on ice. Mix each solution by vortexing.
2. Assemble the following components in a tube on ice, and mix well:

1. Heat the mixture at 65°C for 5 mins and incubate on ice for at least 1 min.
2. Collect all components by a brief centrifugation and add 1 µl of the EasyScript RTase to the tube.
3. Mix well and collect all the components by a brief centrifugation.
4. Incubate the tube at room temperature for 10 min for annealing.
5. Perform cDNA synthesis by incubating the tube for 50 min at 42°C.
6. Stop the reaction by heating it at 85°C for 5 min.
7. Chill on ice. The newly synthesized first-strand cDNA is ready for immediate downstream applications. 

Bullseye EasyScript cDNA Synthesis Kit

Application

• First strand cDNA synthesis for PCR
• Construction of cDNA libraries
• Generation of probes for hybridization

EasyScript cDNA Synthesis Kit is a complete system for the efficient synthesis of first strand cDNA from RNA templates. The recombinant RNasin Ribonuclease Inhibitor, supplied with the kit effectively protects RNA template from degradation.

The kit is also supplied with both oligo(dT) and random primers. The oligo(dT) anneals selectively on the poly(A) tail of mRNA. Random primers do not require the presence of poly(A). Therefore, they can be used for transcription of the 5'-end regions of mRNA. Gene-specific primers may also be used with the kit. The first strand of cDNA can be directly used as a template in PCR.

EasyScript Reverse Transcriptase (RTase) within the kit is a genetically modified form of Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV). Both the EasyScript and EasyScript Plus are RNase H deficient (negative). The enzyme is purified from bacteria host as a single holoenzyme of 71 kDa with the capacity of first-strand cDNA synthesis of template up to 9kb and can be used for routine cDNA synthesis.

Kit Components

Storage Buffer
 
50 mM Tris-HCl (pH 8.3), 100 mM NaCl, 0.1 mM EDTA, 5 mM DTT, 0.1% (v/v) Triton X-100, and 50% (v/v) glycerol.

Storage
 
Store at -20°C in a frost-free freezer. Multiple freezing and thawing of RNA should be avoided. Keep RNA on ice all the time. It is recommended that the first strand cDNA synthesis is carried out under conditions where RNase contamination has been eliminated.

General Protocol

RT-PCR reactions should be assembled in a RNA-free environment. The use of "clean", automatic pipettes designated for PCR and aerosol resistant barrier tips are recommended. 
1. Thaw template RNA and all reagents on ice. Mix each solution by vortexing, and centrifuge briefly to collect residual liquid from the sides of the tubes.
2. Prepare the following reaction mixture in a PCR tube on ice:

3. Incubate at 25°C for 10 minutes if random primer is used. Omit this step if Oligo(dT) primer or sequence specific primer are used.
4. Incubate the mixture at 42°C for 60 minutes.
5. Stop the reaction by heating at 85°C for 5 minutes.
6. Chill on ice. The newly synthesized first-strand cDNA now can be used directly for PCR amplification.

Notes
 
1. Isolation of poly(A)+ RNA from total RNA is not mandatory; however, doing so may improve the yield and purity of the final product.
2. RNA sample must be free of contaminating genomic DNA.
3. Unlike the oligo(dT) priming, which usually requires no optimization, the ratio of a random primer to RNA is critical in terms of the average length of cDNA synthesized in the reaction. Increasing the ratio of random primer/RNA will result in higher yield of shorter (~500 bp) cDNA, whereas decreasing this ratio will produce longer products.

4. The synthesized cDNA should be stored at -20°C.

Bullseye EasyScript™ Reverse Transcriptase

Application

• Synthesis cDNA froma single-stranded RNA or DNA primer extension
• Sequencing dsDNA
• cDNA library
• Template production for use in PCR
• 3'-end labeling of duplex DNA via end-filling reactions

EasyScript™ Reverse Transcriptase is a genetically modified form of Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV). Both the EasyScript™ and EasyScript™ Plus are RNase H deficient (negative). The enzyme is purified from bacteria h o s t as a single holoenzyme of 71 kDa with the capacity of first-strand cDNA synthesis of template up to 9kb and can be used for routine cDNA synthesis.

Kit Components

Storage Buffer
 
50 mM Tris-HCl (pH 8.3), 100 mM NaCl, 0.1 mM EDTA, 5 mM DTT, 0.1% (v/v) Triton X-100, and 50% (v/v) glycerol.

Storage
 
Store at -20°C in a frost-free freezer. Multiple freezing and thawing of RNA should be avoided. Keep RNA on ice all the time. It is recommended that the first strand cDNA synthesis is carried out under conditions where RNase contamination has been eliminated.

General Protocol

RT-PCR reactions should be assembled in a RNA-free environment. The use of "clean", automatic pipettes designated for PCR and aerosol resistant barrier tips are recommended.

1. Thaw template RNA and all reagents on ice. Mix each solution by vortexing, and centrifuge briefly to collect residual liquid from the sides of the tubes. 
2. Prepare the following reaction mixture in a PCR tube on ice:

3. Incubate at 25°C for 10 minutes if random primer is used. Omit this step if Oligo(dT) primer or sequence specific primer are used.
4. Incubate the mixture at 42°C for 60 minutes.
5. Stop the reaction by heating at 85°C for 5 minutes. 
6. Chill on ice. The newly synthesized first-strand cDNA now can be used directly for PCR amplification.

Notes:
1. Isolation of poly(A)+ RNA from total RNA is not mandatory; however, doing so may improve the yield and purity of the final product. 
2. RNA sample must be free of contaminating genomic DNA.
3. Unlike the oligo(dT) priming, which usually requires no optimization, the ratio of a randomprimer to RNA is critical in terms of the average length of cDNA synthesized in the reaction. Increasing the ratio of random primer/RNA will result in higher yield of shorter (~500 bp) cDNA, whereas decreasing this ratio will produce longer products.

4. The synthesized cDNA should be stored at -20°C.